LOCUS       DASJOX010000001        87904 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJOX010000001 DASJOX010000000
VERSION     DASJOX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.001"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..763
                     /locus_tag="U4A12_000001"
     CDS             2..763
                     /locus_tag="U4A12_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEN1772418.1"
                     /translation="MFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISEVTLDET
                     DDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDT
                     ILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEIL
                     KKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRH
                     SLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            830..1324
                     /locus_tag="U4A12_000002"
     CDS             830..1324
                     /locus_tag="U4A12_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1772419.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1380..2018
                     /locus_tag="U4A12_000003"
     CDS             1380..2018
                     /locus_tag="U4A12_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772420.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2090..2485
                     /locus_tag="U4A12_000004"
     CDS             2090..2485
                     /locus_tag="U4A12_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1772421.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2469..4922
                     /locus_tag="U4A12_000005"
     CDS             2469..4922
                     /locus_tag="U4A12_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1772422.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4919..6946
                     /locus_tag="U4A12_000006"
     CDS             4919..6946
                     /locus_tag="U4A12_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772423.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6943..7965
                     /locus_tag="U4A12_000007"
     CDS             6943..7965
                     /locus_tag="U4A12_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1772424.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7982..8911
                     /locus_tag="U4A12_000008"
     CDS             7982..8911
                     /locus_tag="U4A12_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1772425.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9156..9272
                     /locus_tag="U4A12_000009"
     CDS             9156..9272
                     /locus_tag="U4A12_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1772426.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9288..10091
                     /locus_tag="U4A12_000010"
     CDS             9288..10091
                     /locus_tag="U4A12_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1772427.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10326..10562
                     /locus_tag="U4A12_000011"
     CDS             10326..10562
                     /locus_tag="U4A12_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772428.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10569..12521
                     /locus_tag="U4A12_000012"
     CDS             10569..12521
                     /locus_tag="U4A12_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1772429.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12593..13090)
                     /gene="dfrG"
                     /locus_tag="U4A12_000013"
     CDS             complement(12593..13090)
                     /gene="dfrG"
                     /locus_tag="U4A12_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1772430.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            13452..14492
                     /locus_tag="U4A12_000014"
     CDS             13452..14492
                     /locus_tag="U4A12_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1772431.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            14590..14778
                     /locus_tag="U4A12_000015"
     CDS             14590..14778
                     /locus_tag="U4A12_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1772432.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(14836..15189)
                     /locus_tag="U4A12_000016"
     CDS             complement(14836..15189)
                     /locus_tag="U4A12_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772433.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15720..16142
                     /locus_tag="U4A12_000017"
     CDS             15720..16142
                     /locus_tag="U4A12_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1772434.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            16139..16369
                     /locus_tag="U4A12_000018"
     CDS             16139..16369
                     /locus_tag="U4A12_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772435.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            16867..17067
                     /locus_tag="U4A12_000019"
     CDS             16867..17067
                     /locus_tag="U4A12_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1772436.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            17094..18287
                     /locus_tag="U4A12_000020"
     CDS             17094..18287
                     /locus_tag="U4A12_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1772437.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(18355..19920)
                     /gene="guaA"
                     /locus_tag="U4A12_000021"
     CDS             complement(18355..19920)
                     /gene="guaA"
                     /locus_tag="U4A12_000021"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1772438.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(19979..20902)
                     /locus_tag="U4A12_000022"
     CDS             complement(19979..20902)
                     /locus_tag="U4A12_000022"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1772439.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            21098..21703
                     /locus_tag="U4A12_000023"
     CDS             21098..21703
                     /locus_tag="U4A12_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1772440.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            22011..23027
                     /locus_tag="U4A12_000024"
     CDS             22011..23027
                     /locus_tag="U4A12_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1772441.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            23168..24469
                     /locus_tag="U4A12_000025"
     CDS             23168..24469
                     /locus_tag="U4A12_000025"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1772442.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            24515..25177
                     /gene="deoC"
                     /locus_tag="U4A12_000026"
     CDS             24515..25177
                     /gene="deoC"
                     /locus_tag="U4A12_000026"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1772443.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            25198..25596
                     /locus_tag="U4A12_000027"
     CDS             25198..25596
                     /locus_tag="U4A12_000027"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1772444.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            25662..26744
                     /locus_tag="U4A12_000028"
     CDS             25662..26744
                     /locus_tag="U4A12_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1772445.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            26956..28518
                     /locus_tag="U4A12_000029"
     CDS             26956..28518
                     /locus_tag="U4A12_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772446.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            28511..29644
                     /locus_tag="U4A12_000030"
     CDS             28511..29644
                     /locus_tag="U4A12_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772447.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            29644..30600
                     /locus_tag="U4A12_000031"
     CDS             29644..30600
                     /locus_tag="U4A12_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772448.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            30699..31865
                     /gene="deoB"
                     /locus_tag="U4A12_000032"
     CDS             30699..31865
                     /gene="deoB"
                     /locus_tag="U4A12_000032"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1772449.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            31889..32707
                     /locus_tag="U4A12_000033"
     CDS             31889..32707
                     /locus_tag="U4A12_000033"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1772450.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            32724..33431
                     /gene="deoD"
                     /locus_tag="U4A12_000034"
     CDS             32724..33431
                     /gene="deoD"
                     /locus_tag="U4A12_000034"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1772451.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(33729..34415)
                     /gene="gpmA"
                     /locus_tag="U4A12_000035"
     CDS             complement(33729..34415)
                     /gene="gpmA"
                     /locus_tag="U4A12_000035"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1772452.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(34587..35168)
                     /locus_tag="U4A12_000036"
     CDS             complement(34587..35168)
                     /locus_tag="U4A12_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1772453.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(35301..35984)
                     /gene="rpiA"
                     /locus_tag="U4A12_000037"
     CDS             complement(35301..35984)
                     /gene="rpiA"
                     /locus_tag="U4A12_000037"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN1772454.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            36303..36716
                     /gene="rpsL"
                     /locus_tag="U4A12_000038"
     CDS             36303..36716
                     /gene="rpsL"
                     /locus_tag="U4A12_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN1772455.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            36800..37270
                     /gene="rpsG"
                     /locus_tag="U4A12_000039"
     CDS             36800..37270
                     /gene="rpsG"
                     /locus_tag="U4A12_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN1772456.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            37364..39451
                     /gene="fusA"
                     /locus_tag="U4A12_000040"
     CDS             37364..39451
                     /gene="fusA"
                     /locus_tag="U4A12_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1772457.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            39623..40810
                     /gene="tuf"
                     /locus_tag="U4A12_000041"
     CDS             39623..40810
                     /gene="tuf"
                     /locus_tag="U4A12_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1772458.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            41217..41525
                     /gene="rpsJ"
                     /locus_tag="U4A12_000042"
     CDS             41217..41525
                     /gene="rpsJ"
                     /locus_tag="U4A12_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN1772459.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            41556..42185
                     /gene="rplC"
                     /locus_tag="U4A12_000043"
     CDS             41556..42185
                     /gene="rplC"
                     /locus_tag="U4A12_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN1772460.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            42213..42836
                     /gene="rplD"
                     /locus_tag="U4A12_000044"
     CDS             42213..42836
                     /gene="rplD"
                     /locus_tag="U4A12_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN1772461.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            42836..43126
                     /gene="rplW"
                     /locus_tag="U4A12_000045"
     CDS             42836..43126
                     /gene="rplW"
                     /locus_tag="U4A12_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN1772462.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            43165..43998
                     /gene="rplB"
                     /locus_tag="U4A12_000046"
     CDS             43165..43998
                     /gene="rplB"
                     /locus_tag="U4A12_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN1772463.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            44041..44319
                     /gene="rpsS"
                     /locus_tag="U4A12_000047"
     CDS             44041..44319
                     /gene="rpsS"
                     /locus_tag="U4A12_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN1772464.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            44342..44689
                     /gene="rplV"
                     /locus_tag="U4A12_000048"
     CDS             44342..44689
                     /gene="rplV"
                     /locus_tag="U4A12_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN1772465.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            44703..45359
                     /gene="rpsC"
                     /locus_tag="U4A12_000049"
     CDS             44703..45359
                     /gene="rpsC"
                     /locus_tag="U4A12_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN1772466.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            45362..45796
                     /gene="rplP"
                     /locus_tag="U4A12_000050"
     CDS             45362..45796
                     /gene="rplP"
                     /locus_tag="U4A12_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN1772467.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            45786..45974
                     /gene="rpmC"
                     /locus_tag="U4A12_000051"
     CDS             45786..45974
                     /gene="rpmC"
                     /locus_tag="U4A12_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN1772468.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            45999..46265
                     /gene="rpsQ"
                     /locus_tag="U4A12_000052"
     CDS             45999..46265
                     /gene="rpsQ"
                     /locus_tag="U4A12_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN1772469.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            46323..46691
                     /gene="rplN"
                     /locus_tag="U4A12_000053"
     CDS             46323..46691
                     /gene="rplN"
                     /locus_tag="U4A12_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN1772470.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            46728..47036
                     /gene="rplX"
                     /locus_tag="U4A12_000054"
     CDS             46728..47036
                     /gene="rplX"
                     /locus_tag="U4A12_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN1772471.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            47064..47603
                     /gene="rplE"
                     /locus_tag="U4A12_000055"
     CDS             47064..47603
                     /gene="rplE"
                     /locus_tag="U4A12_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN1772472.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            47622..47807
                     /locus_tag="U4A12_000056"
     CDS             47622..47807
                     /locus_tag="U4A12_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN1772473.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            47843..48241
                     /gene="rpsH"
                     /locus_tag="U4A12_000057"
     CDS             47843..48241
                     /gene="rpsH"
                     /locus_tag="U4A12_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN1772474.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            48273..48809
                     /gene="rplF"
                     /locus_tag="U4A12_000058"
     CDS             48273..48809
                     /gene="rplF"
                     /locus_tag="U4A12_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN1772475.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            48970..49326
                     /gene="rplR"
                     /locus_tag="U4A12_000059"
     CDS             48970..49326
                     /gene="rplR"
                     /locus_tag="U4A12_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN1772476.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            49347..49847
                     /gene="rpsE"
                     /locus_tag="U4A12_000060"
     CDS             49347..49847
                     /gene="rpsE"
                     /locus_tag="U4A12_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN1772477.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            49862..50041
                     /gene="rpmD"
                     /locus_tag="U4A12_000061"
     CDS             49862..50041
                     /gene="rpmD"
                     /locus_tag="U4A12_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN1772478.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            50085..50525
                     /gene="rplO"
                     /locus_tag="U4A12_000062"
     CDS             50085..50525
                     /gene="rplO"
                     /locus_tag="U4A12_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN1772479.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            50525..51820
                     /gene="secY"
                     /locus_tag="U4A12_000063"
     CDS             50525..51820
                     /gene="secY"
                     /locus_tag="U4A12_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN1772480.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            51882..52529
                     /locus_tag="U4A12_000064"
     CDS             51882..52529
                     /locus_tag="U4A12_000064"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN1772481.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            52720..52938
                     /gene="infA"
                     /locus_tag="U4A12_000065"
     CDS             52720..52938
                     /gene="infA"
                     /locus_tag="U4A12_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN1772482.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            52971..53087
                     /gene="rpmJ"
                     /locus_tag="U4A12_000066"
     CDS             52971..53087
                     /gene="rpmJ"
                     /locus_tag="U4A12_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN1772483.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            53105..53470
                     /gene="rpsM"
                     /locus_tag="U4A12_000067"
     CDS             53105..53470
                     /gene="rpsM"
                     /locus_tag="U4A12_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN1772484.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            53498..53887
                     /gene="rpsK"
                     /locus_tag="U4A12_000068"
     CDS             53498..53887
                     /gene="rpsK"
                     /locus_tag="U4A12_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN1772485.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            53967..54905
                     /locus_tag="U4A12_000069"
     CDS             53967..54905
                     /locus_tag="U4A12_000069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN1772486.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            54933..55313
                     /gene="rplQ"
                     /locus_tag="U4A12_000070"
     CDS             54933..55313
                     /gene="rplQ"
                     /locus_tag="U4A12_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN1772487.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            55585..56424
                     /locus_tag="U4A12_000071"
     CDS             55585..56424
                     /locus_tag="U4A12_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1772488.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            56400..57269
                     /locus_tag="U4A12_000072"
     CDS             56400..57269
                     /locus_tag="U4A12_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1772489.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            57259..58056
                     /locus_tag="U4A12_000073"
     CDS             57259..58056
                     /locus_tag="U4A12_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1772490.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            58259..59005
                     /gene="truA"
                     /locus_tag="U4A12_000074"
     CDS             58259..59005
                     /gene="truA"
                     /locus_tag="U4A12_000074"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1772491.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(59066..59272)
                     /locus_tag="U4A12_000075"
     CDS             complement(59066..59272)
                     /locus_tag="U4A12_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772492.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(59338..62007)
                     /locus_tag="U4A12_000076"
     CDS             complement(59338..62007)
                     /locus_tag="U4A12_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN1772493.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(62245..62433)
                     /locus_tag="U4A12_000077"
     CDS             complement(62245..62433)
                     /locus_tag="U4A12_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN1772494.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            62703..63065
                     /locus_tag="U4A12_000078"
     CDS             62703..63065
                     /locus_tag="U4A12_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1772495.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            63117..64799
                     /locus_tag="U4A12_000079"
     CDS             63117..64799
                     /locus_tag="U4A12_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN1772496.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            64916..66952
                     /gene="recG"
                     /locus_tag="U4A12_000080"
     CDS             64916..66952
                     /gene="recG"
                     /locus_tag="U4A12_000080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN1772497.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            67001..68002
                     /gene="plsX"
                     /locus_tag="U4A12_000081"
     CDS             67001..68002
                     /gene="plsX"
                     /locus_tag="U4A12_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN1772498.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            68124..68366
                     /gene="acpP"
                     /locus_tag="U4A12_000082"
     CDS             68124..68366
                     /gene="acpP"
                     /locus_tag="U4A12_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1772499.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            68634..69638
                     /locus_tag="U4A12_000083"
     CDS             68634..69638
                     /locus_tag="U4A12_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772500.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            69639..70583
                     /locus_tag="U4A12_000084"
     CDS             69639..70583
                     /locus_tag="U4A12_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772501.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            70583..71545
                     /locus_tag="U4A12_000085"
     CDS             70583..71545
                     /locus_tag="U4A12_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772502.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            71572..72486
                     /locus_tag="U4A12_000086"
     CDS             71572..72486
                     /locus_tag="U4A12_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772503.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            72512..74293
                     /locus_tag="U4A12_000087"
     CDS             72512..74293
                     /locus_tag="U4A12_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1772504.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            74641..75327
                     /locus_tag="U4A12_000088"
     CDS             74641..75327
                     /locus_tag="U4A12_000088"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN1772505.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            75347..78928
                     /gene="smc"
                     /locus_tag="U4A12_000089"
     CDS             75347..78928
                     /gene="smc"
                     /locus_tag="U4A12_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN1772506.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            78937..79746
                     /locus_tag="U4A12_000090"
     CDS             78937..79746
                     /locus_tag="U4A12_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1772507.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            79757..80755
                     /gene="ftsY"
                     /locus_tag="U4A12_000091"
     CDS             79757..80755
                     /gene="ftsY"
                     /locus_tag="U4A12_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN1772508.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            80942..83209
                     /gene="gshAB"
                     /locus_tag="U4A12_000092"
     CDS             80942..83209
                     /gene="gshAB"
                     /locus_tag="U4A12_000092"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN1772509.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(83298..83750)
                     /locus_tag="U4A12_000093"
     CDS             complement(83298..83750)
                     /locus_tag="U4A12_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN1772510.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNESVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            83900..84850
                     /locus_tag="U4A12_000094"
     CDS             83900..84850
                     /locus_tag="U4A12_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1772511.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            84843..85802
                     /locus_tag="U4A12_000095"
     CDS             84843..85802
                     /locus_tag="U4A12_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1772512.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            85799..86554
                     /locus_tag="U4A12_000096"
     CDS             85799..86554
                     /locus_tag="U4A12_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1772513.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            86577..87530
                     /locus_tag="U4A12_000097"
     CDS             86577..87530
                     /locus_tag="U4A12_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1772514.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        28909 a        15670 c        18608 g        24717 t
ORIGIN      
        1 tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc
       61 ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac
      121 tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct
      181 ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa
      241 agatttgatt acccagacag caagctccat atatgataaa caaggtgata atgtattagt
      301 ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa
      361 aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga
      421 atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact
      481 aaaaaaatat gaaattacag ttcctagaga cgaaaatcta tctaaattat atagtaggat
      541 acaacaatta attgaaaaag aaattcaacc tactgaactt gctgatatag tgaagacaac
      601 gataaggtca tcaaatggaa tgattagctc tcttaatgaa gcaagaaata ggcattcact
      661 tgcacaccct aatacaaata ttattggaaa gagagaagca aaactaataa taggtatttc
      721 aagtacagtt acagattata ttagcggata ccttgataag tagcgtcatt aactgattgc
      781 tacttttttt atgactattt ttataaaaag gatgtgataa gaattggaaa tgaaagtcta
      841 tgtggcaaat ttaggacggt ataatgaagg tgaattagtc ggagcatggt ttactccacc
      901 aattgatgag gaagaaatgg cagaaagaat cggtttaaat gaggactacg aagaatatgc
      961 aatacatgat tttgagcttc cttttgatgt ggacgaatac acgccgatta gtgaaatcaa
     1021 ccgtctatgt gaagccattc aagaaatcga aggtacacct atttataatg aactcaaaga
     1081 aattcaaggc atgtggttta gtagcttgga agaattactg gaaaacaaag aggatattca
     1141 ttgttatagt gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg
     1201 acagcttggt gaagtccctt ccaatttaca aaattatatt gactaccaat ctttgggacg
     1261 tgatatggaa atagagggta attacctagt tacctctcat ggggtatttg aatattgtca
     1321 gtaagtttaa caatccgtgt tataattaaa ttaataaata gcacggagag gtataaatta
     1381 tggtaaatgt atttgaaaat cttaaactat taaaactaga catgaaagat aaaggctggg
     1441 tgatagattc cttttacttt cgttacaaac agcaaaatta tattgtactt gttaagctat
     1501 ttgaaaaaga ggaaaaagtt ccagaatatg ctctgttaaa actagaattt ttaaaggaaa
     1561 acgatttttc agatatgcta gctgtctatg ctaattcggt aaaattatat acagacacga
     1621 aaacaataag agaatatttt ggtatcgaat acagtagtaa cttgggagat gttttatttc
     1681 aatttagcca aactcttgct cgatttattc cgacagaagt tagtgagaag aaaaatgaag
     1741 accaaaaaga agccatgtgt ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa
     1801 aatattgttt ttcagttaga agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa
     1861 gtccttttaa tgacaataaa acgagattgt atagacccag cttatatgaa cgattagggt
     1921 cagataccaa tttaagtttc agatattcaa tgaatccaga tgatgaagaa actgatgaag
     1981 gtattattgc aaaatggaca aaaaataaaa ttgaatagtt aaagcattga aagagcaatc
     2041 acctattttg attgttcttt ttctattttt acggaaagga cgtgaagaca tgaaaaaaat
     2101 aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcgatta atgatttaca
     2161 actccctttc ccaattacct ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt
     2221 catactcttt ggcagtttac caccactttc tctgattgac ggagcactac tcaaatactt
     2281 agggattccc attgttctca ctgggttcat gtgccaaaag acctttgacg gaaagaaacc
     2341 ctatggattt ctaaagtcta tcattaccta tgctctacgc cctaaagtaa cctacgctgg
     2401 caaacaaata cgattaaaaa aagaaacaat gaatgaatcg attacgattg taaggagtga
     2461 acaactagat ggcatatccg attaaatata tggaagataa tctcgtattt aatcatgatg
     2521 gcgaagtatt cgcttattat gaattaattc cctataatta cagtttttta agttccgatg
     2581 aaaaaattca agttcatgat aatttctgtc aattgattgc ccaaaatcgt gatggcaaga
     2641 ttcatgctct gcaactagca accgaatcaa gtattcgagc cacacaggaa caatcgaaaa
     2701 aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa cagaccgaag
     2761 cgttaatttc tatgattggt gacaatcaag tagactatcg cttttttatt ggttttaaac
     2821 tctacctcaa tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat
     2881 ttgcggaatt ctttcatggt gtgaatcaca aattaatggg cgattttgtt tctatgccta
     2941 tggcggaaat cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa ttatccagac
     3001 gctttcagat tcgtaaattg gagaaagatg attttggtta tattattgaa catctttacg
     3061 ggcaaacagg tgtccctttt gatgactacg actaccattt accgaaacga tacgaagata
     3121 aaaaagtgtt ggtaaagaaa tacgatattc tcaaacccac tcgctgttta atcgaagaaa
     3181 atcaacgcta tttacggatt gaacaggaag ataccactac ttatgtggcg tattttacaa
     3241 ttaatagtat tgttggtgaa ttggatttcc ctaatagtga aattttttat taccagcaac
     3301 agcagtttac tttccccgtt gataccagta tgaatgttga aattgtcgcc aataaaaaag
     3361 ccttatctac cgttcgcaat aaaaagaaag aactgaaaga tttagatagc cacgcatggg
     3421 aaagcgacaa cgaaaccagt actaatgtgg tggacgcttt agaatctgtc gatgaattag
     3481 aaagtaattt agaccaaacc aaagaatcta tgtacaaact aagctatgtg gtacgggtat
     3541 cagcaaagaa tttggacgag ctgaaacaac gttgtgatga agtaaaagat ttttatgatg
     3601 atctgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat aatgaattta
     3661 ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg gattttttgg
     3721 ctggtcttgg tttcggtgct acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt
     3781 acaatctcga tacaggcaga aatatttatc tccaacctag ccttgcaagt caaggaatta
     3841 aaggctctgt aacaaatgca ttagcgtccg cttttattgg ttcattaggt ggcggaaaat
     3901 cattctctaa caatatgctg gtctattatt cagtactctt tggcggacag gcaaccattg
     3961 ttgacccaaa agctgaacgt ggtgaatgga aagaaacctt acctgatatt gcccatgaaa
     4021 tcaatattgt taatctcacg agtgagaacg acaataaagg gttacttgac ccttatgtga
     4081 ttttaaaact cccgaaagat tcagaaagcc ttgccattga tattctcacg ttcttaacag
     4141 gtatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa
     4201 ctcaaagcaa acaacgaggg ttgttattgg tgattgatga attgcgaaaa gaagatacgc
     4261 ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat tttgcccatc
     4321 tgttattcag tgatggcagt gtcacccaat ccattagctt ggaaaaacaa ctcaatatta
     4381 ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa tacactacca
     4441 tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta gactttatcc
     4501 actctgaccg ttccattttt aaaattgttg accttgatga ggcttggagt tttttacaag
     4561 tggcacaagg aaaagctcta tctatgcgat tggttcgagc aggacgtgcc atgaatgcag
     4621 gggtctattt cgttactcaa aacgcggacg atttactaga tgaaaagatg aagaataata
     4681 ttggcttgaa atttgccttt cgttccactg acctagtgga aatcaaaaag accttagaat
     4741 tttttggtgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg
     4801 gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt cacccgattt
     4861 ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag gtggaagaat
     4921 gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt gtgtgggcgt
     4981 tattttactt ttattaggca cgctgggcaa tgcagtacaa gcgacaggct tagttgatga
     5041 aaccattgat acaagcaatg aatactccaa gtatggtttg aatcaatatc agcttgatta
     5101 ttatgtggac aatagctggg gctggcttcc atggaattgg agtgatggca taggacaatc
     5161 ggtgatgtat ggattgtatg ccattactaa ttttatttgg acaatttctc tttatctatc
     5221 gaacgccaca ggttatttgg tgaaagaagc ctatgcctta gatttcatta gtcaaacatc
     5281 ccaagcaatt ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg gcttttccag
     5341 ttcagggttt tatgtcggtt tcctccttct ttttattctc attgttggaa tctatgttgc
     5401 ctatacagga ttggttaagc gagaaacgac gaaagtgata cgagctgttt taaactttgt
     5461 cgtggtgttt attctatcgg cttcctttat tgcgtatgca cccgattata ttgcgaaaat
     5521 caatgatttt tccaaagatg tcagtaccgc cagtctgaat attgggacaa aaattgttct
     5581 tcctaacacg gatagtcaag ggaaagacag tgtggacatg atacgtgaca gcttattctc
     5641 tgttcaagta aagcaaccat ggctattgct tcaatatggc tccactgatg tagaagcgtt
     5701 aggggaagaa cgtgtggaaa gtttattgtc taccagtcca gatacaaaca atggtgaaga
     5761 tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt taaccctacc
     5821 gaagacgatt tccagattag gaacagtctt ctttttgttt gtattcaata ttggtatctc
     5881 gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat ttattatttt
     5941 cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg atggcatggg
     6001 caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta ttaccttgat
     6061 tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa gttctccctt
     6121 tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta aactgggcga
     6181 tttaatgagc ctattcactt tacaaagcaa cgactctcaa aacatgggca gacaagtgtt
     6241 tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa
     6301 gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca
     6361 aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc
     6421 actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc
     6481 ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcaa aacaacagat
     6541 acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca
     6601 taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca aacaacagcg
     6661 tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg agctggataa
     6721 agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata accagcagca
     6781 tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa
     6841 tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc aattcaatca
     6901 gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct aaaaataaca
     6961 gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct agtcattttc
     7021 tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctattcc acaaggcggt
     7081 gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata tggtaaggaa
     7141 tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga atcgggcggt
     7201 acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa cagtttgagt
     7261 accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac cagtgcagaa
     7321 caacaaggag tggatataga ttctgttatc caatcttata attatggcgg tggcttttta
     7381 aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa
     7441 gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt
     7501 ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc
     7561 gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg
     7621 aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt
     7681 agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa
     7741 gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc
     7801 ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag
     7861 ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat
     7921 tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca
     7981 aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc
     8041 gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt
     8101 tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat
     8161 tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca
     8221 agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga
     8281 agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt
     8341 aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt
     8401 gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt
     8461 tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat
     8521 agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa
     8581 atcggactac caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga
     8641 cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga
     8701 attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga
     8761 ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt
     8821 gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga
     8881 aaaacaagat aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt
     8941 tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg
     9001 tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa
     9061 ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta
     9121 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata
     9181 tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtgataaa agtatttatc
     9241 actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta
     9301 atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat
     9361 ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata
     9421 atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt
     9481 gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag
     9541 tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg
     9601 tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct
     9661 tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta
     9721 aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt
     9781 gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc
     9841 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
     9901 gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata
     9961 tagggattga taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag
    10021 gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc
    10081 ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac ctaaatgagt
    10141 atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa
    10201 tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa ataattattg
    10261 taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt
    10321 ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat tgggaaatat
    10381 cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt actgaaagcg
    10441 aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt gaatggattg
    10501 ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat agagtgggtt
    10561 gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt gtagatgtta
    10621 aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat ttattaactc
    10681 caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct tttaaatgga
    10741 acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt tgtaattttg
    10801 ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat aaaatgactg
    10861 gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct attggcagag
    10921 gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt gaagaaatta
    10981 aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa tttcataaag
    11041 aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg
    11101 ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag ttcactaatg
    11161 ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc attcttccaa
    11221 tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat attttaggaa
    11281 ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt ttggagtttt
    11341 tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg tggcttaata
    11401 cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat
    11461 atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct gaggtcttat
    11521 caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg gatgaattaa
    11581 atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt aaaaatgata
    11641 ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa aataggaatg
    11701 cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat ggtttaaatg
    11761 ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg gttaatgccg
    11821 acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt tatagagtat
    11881 tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt aacaagacga
    11941 aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg ctcgattggg
    12001 gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat tatctaactg
    12061 aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga gaaagatatc
    12121 gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa ggattttatt
    12181 cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca aagatgctat
    12241 taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt tatatatcat
    12301 ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt tacgctaact
    12361 tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac aacttttgtc
    12421 gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta gggattacag
    12481 ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag cttggagtaa
    12541 tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc cgttaactca
    12601 ataagttttt tctttcatat acatgaaaat agtagttata cggattttta tcattcttta
    12661 tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga aaaaaagtat
    12721 ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa gggaaaaaca
    12781 aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta cataactcaa
    12841 aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc ttatctctcg
    12901 tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg ttcttcctac
    12961 ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc ttgggaatcc
    13021 tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca gcaatcaaag
    13081 aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc ttatttctat
    13141 caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt tctataagtt
    13201 atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt caattgcatt
    13261 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
    13321 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
    13381 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
    13441 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
    13501 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
    13561 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
    13621 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
    13681 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
    13741 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
    13801 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
    13861 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
    13921 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
    13981 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
    14041 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
    14101 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
    14161 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
    14221 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
    14281 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
    14341 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
    14401 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
    14461 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
    14521 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
    14581 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
    14641 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
    14701 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
    14761 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
    14821 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
    14881 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
    14941 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
    15001 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
    15061 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
    15121 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
    15181 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
    15241 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
    15301 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
    15361 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
    15421 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
    15481 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
    15541 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
    15601 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
    15661 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
    15721 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
    15781 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
    15841 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
    15901 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
    15961 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
    16021 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
    16081 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
    16141 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
    16201 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
    16261 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
    16321 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
    16381 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
    16441 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
    16501 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
    16561 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
    16621 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
    16681 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
    16741 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta ttattataaa
    16801 actatttgat ataattatag gtgaatattg gagttttcct agcctataat tgggaaagga
    16861 gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg aacttctttt
    16921 tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca tatctactat
    16981 caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct aggaattcgg
    17041 ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa actatggtta
    17101 aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat ctaggttatg
    17161 attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa aaagaagcaa
    17221 gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag aataaggata
    17281 aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag acacaggtaa
    17341 agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct tattttgata
    17401 aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta aaattatcaa
    17461 agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt gctatggatc
    17521 gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc aatgtgaaaa
    17581 agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta atctctctaa
    17641 tttacaaaga agattactat caacactttt tatttatctc tctgtggttt ttatttatga
    17701 ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat tttgactcag
    17761 gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat agatttgttg
    17821 aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca ttaacttatt
    17881 tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta atgagctata
    17941 acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat gctaagctgg
    18001 ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc ttgttaatca
    18061 gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat attgagacga
    18121 ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct aacaaattaa
    18181 atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca aagaatcaat
    18241 ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa
    18301 agataagaag agccagaaac ccttggtata agaggatttc tagctctcct acaactattc
    18361 ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc
    18421 tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa
    18481 gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt
    18541 acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca
    18601 gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg
    18661 tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc
    18721 agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg
    18781 gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg
    18841 cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat
    18901 aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt
    18961 tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc
    19021 tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga ctttgatgat
    19081 gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag
    19141 cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac
    19201 cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac
    19261 ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc
    19321 acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg
    19381 tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat
    19441 cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc
    19501 accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc
    19561 gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc
    19621 gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa
    19681 attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc
    19741 agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct
    19801 caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg
    19861 actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa
    19921 atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct
    19981 aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat
    20041 cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc
    20101 atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat
    20161 aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt
    20221 tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt
    20281 agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag
    20341 gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag
    20401 gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca
    20461 tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat
    20521 ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag
    20581 acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga
    20641 tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga
    20701 acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat
    20761 ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt
    20821 gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt
    20881 agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt
    20941 aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc
    21001 tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata
    21061 gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga
    21121 acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt
    21181 tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt
    21241 cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg
    21301 atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat
    21361 ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat
    21421 cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc
    21481 agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac
    21541 aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca
    21601 aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac
    21661 aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg
    21721 gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt
    21781 tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag
    21841 tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc
    21901 ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt
    21961 gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga
    22021 ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga
    22081 ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg
    22141 aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca
    22201 tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag
    22261 agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag
    22321 aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg
    22381 ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc
    22441 caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg
    22501 aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg
    22561 gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc
    22621 atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga
    22681 ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta
    22741 ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca
    22801 aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca
    22861 tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac
    22921 gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa
    22981 caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat
    23041 tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt
    23101 tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga
    23161 tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa
    23221 agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt
    23281 cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt
    23341 aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa
    23401 ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct
    23461 agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg
    23521 tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga
    23581 atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc
    23641 accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc
    23701 gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt
    23761 ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc
    23821 aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga
    23881 tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga
    23941 aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca
    24001 aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga
    24061 agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg
    24121 agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct
    24181 tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc
    24241 aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt
    24301 gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta
    24361 tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg
    24421 tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc
    24481 ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca
    24541 tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa
    24601 aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca
    24661 attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac
    24721 acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga
    24781 tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat
    24841 cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc
    24901 gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga
    24961 ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt
    25021 gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga
    25081 agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt
    25141 agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg
    25201 gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt
    25261 ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa
    25321 ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt
    25381 ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc
    25441 cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct
    25501 gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca
    25561 ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac
    25621 tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat
    25681 ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg
    25741 gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca
    25801 cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa
    25861 aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt
    25921 caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga
    25981 tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag
    26041 gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa
    26101 ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa
    26161 caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg
    26221 gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga
    26281 atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct caaggtatgt
    26341 atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac ggtgtattcc
    26401 aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc
    26461 gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac ttcacattga
    26521 catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa tcagcggacg
    26581 gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt gttggtctaa
    26641 cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca
    26701 tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc
    26761 ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg ttcatttatc
    26821 caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg tttgtttcaa
    26881 aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata gaaatcgtta
    26941 ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa tattaccaaa
    27001 gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc aggagaaatt
    27061 catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt gctttccgga
    27121 ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa gatcacaagt
    27181 ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat gcttgttgat
    27241 gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt
    27301 gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg attgtcagtc
    27361 aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt agaaatttta
    27421 aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc tgtattgacc
    27481 cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg
    27541 atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg ctgtacagtt
    27601 atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc acaacaatta
    27661 gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct
    27721 caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc
    27781 gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat
    27841 ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc agaaagcgga
    27901 catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat cactgaacat
    27961 ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga tatgacatta
    28021 tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa cggcatgctg
    28081 aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga tgttcgaaca
    28141 acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa agcaattatc
    28201 gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac tcgtggactg
    28261 gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga caaatacaaa
    28321 gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga tcgtattgct
    28381 gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa
    28441 ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt agaaaagggg
    28501 gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc ctgtactttc
    28561 agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt ttcagcccat
    28621 ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga taggtgaaac
    28681 gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag ctaactcagc
    28741 tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg tcagtgtctg
    28801 ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat gcgtgatctt
    28861 aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag cattttttgg
    28921 aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg taagcacatt
    28981 tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt ccaatatgat
    29041 cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg gctctcgcgt
    29101 aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc tgatgaagaa
    29161 aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt ctgagtatgc
    29221 aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg ctttggcagg
    29281 gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc aaacgacttc
    29341 gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat cagcaatcgg
    29401 tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg gcatgcagac
    29461 aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta tcttctttgt
    29521 cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga
    29581 agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact
    29641 ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt ttattcaaca
    29701 ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat cgtaaacgta
    29761 gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa cttgacgatg
    29821 agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt
    29881 atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga ccatattatc
    29941 agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat caaagcatgg
    30001 tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc tttcccagga
    30061 ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt acctgcttat
    30121 tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa attcggcttg
    30181 cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat caatgtatat
    30241 gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg cggtgctgtt
    30301 tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca agggttcatc
    30361 tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg tgcctcattg
    30421 ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt tatttcttcg
    30481 attccgccag tattgctgca aattgctcca tatttgctga caatcattgt gctggttgtt
    30541 ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa atcaaaataa
    30601 tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt
    30661 ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc gtacatctga
    30721 tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc ggtgatgttg
    30781 gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca catttagagc
    30841 agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag
    30901 gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca ggccactggg
    30961 aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg
    31021 atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac aaaccttata
    31081 gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc gatttaattg
    31141 tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc attcctttag
    31201 aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat ccgtatatga
    31261 ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca
    31321 atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct ttgaaagaag
    31381 caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga caaggaatca
    31441 ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg aaagttatga
    31501 aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca ttatacggtc
    31561 atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta cgaattccag
    31621 aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat cacggaaacg
    31681 atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg gtttacagta
    31741 aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt tcagcaacga
    31801 ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg aaattattaa
    31861 aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt tacaagaaac
    31921 gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga ttctaggttc
    31981 aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt atgatgagat
    32041 cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt atgggtctct
    32101 ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag gtcattccat
    32161 gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt cagtcattgt
    32221 gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga tgctgattac
    32281 cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg
    32341 ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc
    32401 agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct attctggacc
    32461 aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg atgcagtagg
    32521 gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag tgttaggaat
    32581 ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg aagaagttgt
    32641 agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa cattgacatt
    32701 attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg
    32761 ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg gagacttttt
    32821 tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc ggcaaatata
    32881 aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc accatctatg
    32941 cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt acatgcggtg
    33001 cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca gcgaccagtt
    33061 cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt gatttcaatc
    33121 tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat gtaggaaatg
    33181 ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa ttagctgaac
    33241 ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc gcgaaatacc
    33301 atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt gaagaaacaa
    33361 cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg gaaacagcga
    33421 tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga acatccaagc
    33481 aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg taagcttttg
    33541 aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt
    33601 tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgaga agttggcagg
    33661 agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc ccttttttat
    33721 agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa ttcataaaca
    33781 agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat accttcgata
    33841 tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt tttgttgtct
    33901 agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa tgtcactttc
    33961 aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg gtcatttgct
    34021 gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga acgacgccag
    34081 atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa accttgtaat
    34141 ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca aagttgatct
    34201 gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga agtgtaagca
    34261 acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt
    34321 ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc attccattca
    34381 ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt taagataggt
    34441 ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag agatttccat
    34501 cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa acgaacagtg
    34561 cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc tttttcaggt
    34621 aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga aataaattgg
    34681 atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt tgcatcgatt
    34741 tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac taatgtacgt
    34801 tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa atagattttt
    34861 cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc gcgatctttg
    34921 aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg aagttggcaa
    34981 atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg aacagcagta
    35041 gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc aaagtcagat
    35101 aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata
    35161 attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca gtaaaaatgt
    35221 agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct ttttttattg
    35281 gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc aacgcctttt
    35341 tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc tactacccca
    35401 acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc tagatgcaga
    35461 tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc tgtacgcaag
    35521 acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc ataagggatc
    35581 acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga atcaacgatc
    35641 caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc agcgccgccg
    35701 cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt caaatcgact
    35761 acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg ttcttttgtc
    35821 gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc
    35881 tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat cccgtccttt
    35941 acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc gtccaccttt
    36001 ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt aaaaagatac
    36061 cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt
    36121 ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg
    36181 ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat tttttatcac
    36241 aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg aggaaacaag
    36301 gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta
    36361 attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact aacgtaaact
    36421 cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa aaaccgaact
    36481 cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt actgcttata
    36541 tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc ggtggtcgtg
    36601 taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat actgctggtg
    36661 ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa gcttaatttt
    36721 aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat gaacaatatt
    36781 tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc
    36841 agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg
    36901 aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca aagaatctac
    36961 aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga
    37021 agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag ttcgccctga
    37081 acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca
    37141 cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca caggtgcttc
    37201 tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta
    37261 tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag atagtgtcct
    37321 tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca gagaattttc
    37381 tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta aaacgactgc
    37441 tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa ctcatgaagg
    37501 agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga tcacttctgc
    37561 tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac caggacacgt
    37621 agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg taactgttct
    37681 tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag caacaactta
    37741 tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg acttcttata
    37801 ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc aattaccaat
    37861 tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg ctgaaatgta
    37921 tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat accgcgaatt
    37981 agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg aagaacttac
    38041 tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag gtatccgccg
    38101 cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta agaacaaagg
    38161 ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag atatcccagc
    38221 tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag acgacagcgc
    38281 tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc gtttaacttt
    38341 cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg caacaaaagg
    38401 caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt cagaaatctc
    38461 tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa ctactgggga
    38521 cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc cagaaccagt
    38581 tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt
    38641 acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga
    38701 aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc gtatgcgtcg
    38761 tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg aaactttccg
    38821 tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg
    38881 tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc
    38941 gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg gtttgaaaga
    39001 tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag ctaaacttta
    39061 cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat
    39121 ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa tcatggctgt
    39181 agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg
    39241 tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc gtgcgatggt
    39301 tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc aaggtcgtgg
    39361 tacatttaca atgacatttg accactacga agatgtacca aaatcagtac aagaagaaat
    39421 cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt cctgcaaaga
    39481 gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta tcgatgatat
    39541 gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa aatatattgt
    39601 atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta aaccacacgt
    39661 taacattggt actatcggac acgttgacca tggtaaaact acattaacag ctgcaatcac
    39721 aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa tcgatggtgc
    39781 tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt atgaaacaga
    39841 tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta aaaacatgat
    39901 cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg acggcccaat
    39961 gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt
    40021 attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag ttgaaatgga
    40081 agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg tagttgctgg
    40141 atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat
    40201 ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac cattcatgat
    40261 gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag gtcgtgttga
    40321 acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc
    40381 aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg
    40441 agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt
    40501 tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag tatacgtgtt
    40561 gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc cacaattcta
    40621 cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg aaatggttat
    40681 gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta tcgaaaacgg
    40741 tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg ttacagaaat
    40801 caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg taatcgctcg
    40861 gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga tcaggaaaaa
    40921 agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt ttctaaatga
    40981 aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga gtttatatat
    41041 attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc agaatgattt
    41101 cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga acgagcgtga
    41161 cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg
    41221 caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag
    41281 cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt ccaattccat
    41341 taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac aaagattcac
    41401 gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac ccaacaccaa
    41461 aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc gaaatcaaat
    41521 tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc ttagggaaaa
    41581 aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta actgttgtag
    41641 aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc tacgaagcta
    41701 ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg
    41761 ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat gttgagctag
    41821 gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga gacattattg
    41881 atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa
    41941 gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca atgggtccag
    42001 ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg
    42061 taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca
    42121 aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct gtgaaagcta
    42181 aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta ttcaaacaag
    42241 atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt gaacctaatg
    42301 aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg
    42361 cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa
    42421 caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt ggcgtagtct
    42481 ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt cgtttagcaa
    42541 tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc gaaggcttga
    42601 acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta tctatcgact
    42661 ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca gcacgtaact
    42721 taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt gtatcaaata
    42781 caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt gcataatgaa
    42841 cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg ccatggatga
    42901 caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa aacaagcggt
    42961 agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt
    43021 caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga tcgttacatt
    43081 gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac taatagataa
    43141 ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac ctcaaatggt
    43201 cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc tgaaaaatca
    43261 ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat cactgtacgt
    43321 catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg taacaaagat
    43381 aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc taacatcgcg
    43441 ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg actagaagtg
    43501 ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc tcttccatta
    43561 gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg taaaggtgga
    43621 caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg caaatacgta
    43681 ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg tgctacaatc
    43741 ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg acgctctcgt
    43801 tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga tcacccacac
    43861 ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa
    43921 ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact tattgttcgt
    43981 cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag gaggttcacc
    44041 atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa aaaagttgaa
    44101 gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt
    44161 ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca tgtaccagtt
    44221 tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac aagaacttat
    44281 cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag gataaatcca
    44341 aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca cttcacctcg
    44401 aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg caatttcaat
    44461 cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga tgtcagcagt
    44521 tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt
    44581 tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc
    44641 aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag gagggataat
    44701 cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc gactgggatg
    44761 ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta agaatccgta
    44821 aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa
    44881 caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt ggtaaaggcg
    44941 gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa agaatccata
    45001 tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt gaaggaatcg
    45061 cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc atccaacgct
    45121 caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag
    45181 atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact ttacgtgcgg
    45241 acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga gttaaagtgt
    45301 ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga gggaaataat
    45361 catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga
    45421 agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg ttgattcaca
    45481 ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg
    45541 tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca aagcaattgg
    45601 ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg
    45661 taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag cgttacgtct
    45721 agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga
    45781 atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt gatcaagaaa
    45841 aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt caattagaaa
    45901 acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg
    45961 aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga agaaatcaac
    46021 gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt accgttgctg
    46081 tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct aagaaataca
    46141 aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt atggaaactc
    46201 gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa gcagttatta
    46261 tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg aggatacaca
    46321 acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt
    46381 tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt gatgtgatcg
    46441 ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag
    46501 ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat attaaatttg
    46561 atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca cgtatctttg
    46621 ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag
    46681 aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg tttgttaaaa
    46741 aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc gttgtactag
    46801 cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc
    46861 aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg ccaattcatg
    46921 tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc tacaaagaag
    46981 tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat aaataaaagt
    47041 ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa
    47101 ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct aaagttgaaa
    47161 aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac ttagataaag
    47221 cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa gctaaaaaat
    47281 caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg
    47341 gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca cgtgtacgtg
    47401 acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact ttaggtatta
    47461 aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta cgtggtatgg
    47521 acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg ttgacacaat
    47581 taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa aaatcaatga
    47641 ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt
    47701 gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac
    47761 ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc
    47821 ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt ttctaactcg
    47881 tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat caaaaattaa
    47941 acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga
    48001 agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt
    48061 tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca aagctgacga
    48121 agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt
    48181 cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt acgtatggta
    48241 atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg
    48301 ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt aaaggaccaa
    48361 aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag
    48421 taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga acaactcgtg
    48481 cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa
    48541 tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac gttggttact
    48601 cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca gctaacacac
    48661 aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca aacattcgtg
    48721 gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc gaatatgtac
    48781 gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag aagccagcag
    48841 caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc
    48901 agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa aaataaagag
    48961 gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag acatcgtcgt
    49021 gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt tcgttctaac
    49081 aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc aagtgcctct
    49141 accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa
    49201 ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga ccgtggtgga
    49261 tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa
    49321 ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat ttggaattag
    49381 aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac
    49441 gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt ggtacaggta
    49501 aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag aaaaacttag
    49561 ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct tttagtggtg
    49621 gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt ggaccagttc
    49681 gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta ggctcaaata
    49741 caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag
    49801 aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga ggacaacaat
    49861 aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg
    49921 cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta agccttctaa
    49981 tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg aagaaattta
    50041 atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa cttcatgaat
    50101 taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg
    50161 gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg
    50221 tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca aaacgaggat
    50281 tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg aaccgctttg
    50341 aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa
    50401 aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact gtgaaagcag
    50461 caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca atcgaggtga
    50521 tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat cgaaaatcct
    50581 atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg taccaggcgt
    50641 aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt tgaacttggt
    50701 aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac catacattac
    50761 tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc
    50821 gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc tgacattagt
    50881 attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg ctcaattagg
    50941 tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc taacagccgg
    51001 tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg gaaatggcgt
    51061 atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca gagaaattta
    51121 tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta tttttgtggc
    51181 cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc aacaagctga
    51241 acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa gcagctatct
    51301 gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt cgtttattgc
    51361 tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa actggtacaa
    51421 agtgatgatg gagatcttta actataatac agcacctggt gctacgattt atacgattct
    51481 gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga aattagcgga
    51541 aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg ggactgaaga
    51601 gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc ttggtttagt
    51661 ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa tcggattagg
    51721 cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac agttagaagg
    51781 cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc
    51841 cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc attttaatgg
    51901 ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct tatggtattc
    51961 cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca gctcttggcc
    52021 tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta acaaatggaa
    52081 tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta gatggctttc
    52141 cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg aataaaaaaa
    52201 ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt ctagccggcc
    52261 gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct acaaaagttg
    52321 aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat gataaacctg
    52381 agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt
    52441 ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat gctgtatttg
    52501 cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact tgcattatgc
    52561 agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac ttctagagaa
    52621 tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc gccattttat
    52681 cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga agatatgatt
    52741 gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa
    52801 aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta catccgtatc
    52861 ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg tggccgtatt
    52921 acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc atgaaagtaa
    52981 gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta
    53041 tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg gaggtgctat
    53101 aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag tagtttcact
    53161 tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg atgctggtgt
    53221 atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta tccgtgcaga
    53281 agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg
    53341 tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg
    53401 acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta tcgctggcaa
    53461 gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg
    53521 taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt ctacattcaa
    53581 taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt catcagctgg
    53641 ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga
    53701 aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa cagttaaagg
    53761 acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt tagaagtgac
    53821 tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa aacgccgtcg
    53881 tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac aagacacttt
    53941 tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa
    54001 ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga gggtatggga
    54061 ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt gctgccatca
    54121 ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt gttagagaag
    54181 acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat ggccaagaag
    54241 aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc gatattatcg
    54301 ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc tctgaaggag
    54361 ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa
    54421 ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac acacctgttc
    54481 gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat tttgataaat
    54541 tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg
    54601 cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg
    54661 ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg
    54721 aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga atcaatactg
    54781 tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac ttaggacgta
    54841 aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta cgtaaagaag
    54901 attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta ggacgcacat
    54961 ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc aacgaacgta
    55021 ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt
    55081 taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag
    55141 ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta caaaaactgt
    55201 tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc attttgaaga
    55261 cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca
    55321 ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga agatattttt
    55381 ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa gcactcatca
    55441 taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga aggcataaaa
    55501 aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg ttagtaggaa
    55561 ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa gataaattat
    55621 aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac aattgataaa
    55681 ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact tgccaaaacg
    55741 atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca aatattggat
    55801 gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa tccagacaat
    55861 cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa tcaagggatt
    55921 cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag aatggctgag
    55981 tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc
    56041 ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag tatgctagat
    56101 ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga aagtcaattg
    56161 accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg gatactagtg
    56221 atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc tgctggtcct
    56281 gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag cgcactgaaa
    56341 gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt ggattggtta
    56401 tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca tttgaacaaa
    56461 gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca ttagtagggc
    56521 ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt aagccaacaa
    56581 gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat aagaatttaa
    56641 agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag ctattcgaag
    56701 aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag
    56761 cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc tatttggaac
    56821 gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg
    56881 ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat ccccagggca
    56941 gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc actattgtat
    57001 tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat gtgttggaaa
    57061 agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt gaatggctaa
    57121 aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta atggctaaag
    57181 ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct atcgtccatt
    57241 atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat gcctggagat
    57301 tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta ttttatcgga
    57361 atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt cacattactt
    57421 tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg cccattgatc
    57481 tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg agaaacttat
    57541 tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct ctttattttc
    57601 tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac accgcctttg
    57661 gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg gttcccagtc
    57721 cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt gatggacgaa
    57781 acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga aggaaaccta
    57841 gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag cagcttcaac
    57901 cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga
    57961 acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat tgcttttgta
    58021 ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc gctatctatt
    58081 cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa gaaatattag
    58141 ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa
    58201 aattttatag aaaaatcatt attccagata accttttagg aacaaaggag agaaaaacat
    58261 ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat tccaaaaaca
    58321 gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg
    58381 aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg cgatcgggca
    58441 agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga
    58501 tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag aagacttcca
    58561 cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag ggaaaccacg
    58621 cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt tggaaaaaat
    58681 tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt gtgcatcagg
    58741 aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg taaatgaaac
    58801 aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga tgatacggat
    58861 cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata gtatcccaga
    58921 tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg aaggacttta
    58981 tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct tcaagaaata
    59041 agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt attttcgaag
    59101 aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt aactctgagt
    59161 cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg acttgtggac
    59221 gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc aaaaaacgat
    59281 ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat cgtttcttta
    59341 ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg gatcaatcgt
    59401 gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca atgcaataca
    59461 taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga aagtgacagt
    59521 atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac
    59581 tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg tcaccaacat
    59641 ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt ccatttcgac
    59701 acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta gaccaacgac
    59761 tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa atgtcactgt
    59821 tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga tttgcgtttt
    59881 tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg caaaattggt
    59941 tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc aagtgagctc
    60001 ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt
    60061 cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt catttagacc
    60121 agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg gctgctcaac
    60181 atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat tctgatacag
    60241 tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg tgctttcgtt
    60301 tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat tattgttata
    60361 acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt aagaggaaga
    60421 attacgattt cttatagaag aaaacaaact gataccactg taaccatagt tgatcgaatc
    60481 gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat ctttttttgt
    60541 ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc gggaagcata
    60601 attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg cttcagcaga
    60661 catgatcaat aataacaata tactcagatg attgaatgaa agttttttat gctgataaaa
    60721 tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg aagtcatcgt
    60781 cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga gagcagctac
    60841 gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt cccatccttt
    60901 tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata aaaacatatt
    60961 cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac ttgcgataag
    61021 tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc gactggcaaa
    61081 ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa tcgatccgta
    61141 ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat aagaagtcgc
    61201 ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa gatccagcac
    61261 tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga taccataagg
    61321 aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa aatataagaa
    61381 agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga gtaaataact
    61441 gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca aaatcactaa
    61501 tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag tgataaatga
    61561 gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct tgaacgtacg
    61621 tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca gaacatacag
    61681 tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag
    61741 atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg tataaaaaat
    61801 actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa actgtgaaaa
    61861 ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac ttagataaac
    61921 aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc cgttttctct
    61981 aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta tgaagttgta
    62041 ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa
    62101 taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa
    62161 gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat
    62221 ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg
    62281 agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt
    62341 aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt
    62401 cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg
    62461 gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca
    62521 agtacatttt catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt
    62581 atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg
    62641 cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat
    62701 tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg
    62761 caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa
    62821 atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag
    62881 ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc agctatggta
    62941 cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat
    63001 tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag
    63061 actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga
    63121 gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc
    63181 tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata
    63241 cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct
    63301 cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg
    63361 gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg
    63421 ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt
    63481 ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga
    63541 aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg
    63601 taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag
    63661 tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg
    63721 cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg
    63781 atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc
    63841 attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta
    63901 cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg
    63961 tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga
    64021 attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc
    64081 atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac
    64141 gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa
    64201 gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca
    64261 ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga
    64321 acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc
    64381 cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt
    64441 tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca
    64501 ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg
    64561 gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta
    64621 cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag
    64681 gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg
    64741 aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat
    64801 ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga
    64861 tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg
    64921 ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct
    64981 tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga
    65041 agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg
    65101 aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt
    65161 tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa
    65221 agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa
    65281 agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata
    65341 tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca
    65401 agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact
    65461 gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca
    65521 tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg
    65581 attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa
    65641 attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac
    65701 gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga
    65761 tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg
    65821 tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt
    65881 gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc
    65941 aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg
    66001 cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga
    66061 tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc
    66121 agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg
    66181 tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg
    66241 ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag
    66301 aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa
    66361 aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg
    66421 cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga
    66481 caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa
    66541 cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact
    66601 tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa
    66661 aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact
    66721 aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg
    66781 tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag
    66841 agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc
    66901 tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt
    66961 gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc
    67021 aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga
    67081 ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac
    67141 aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc
    67201 agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa
    67261 aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg
    67321 gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc
    67381 agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa
    67441 caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg
    67501 aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag
    67561 tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg
    67621 aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt
    67681 tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt
    67741 aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga
    67801 cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt
    67861 tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta
    67921 tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca
    67981 atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa
    68041 aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg
    68101 tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa
    68161 ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag
    68221 atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat
    68281 tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag
    68341 attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc
    68401 atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttctgat
    68461 aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat ataattatta
    68521 ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat tctgtgtaat
    68581 gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt cgattgtccg
    68641 ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa gatgactatt
    68701 acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta gcaatcgttg
    68761 gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta cttgatccta
    68821 ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc gacctgaacg
    68881 aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa gatccgttgt
    68941 ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt tcctatcaca
    69001 caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa caagtaggga
    69061 ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc ggtatgagac
    69121 aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac
    69181 cgacgacagc cctagatgta accatccaag cacagattct tgatttactg aaagaccttc
    69241 aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc gtagcagaaa
    69301 tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag
    69361 aactgtttga tcatcctaag catccttata cacgctcttt actgaactct ataccacaag
    69421 aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca
    69481 tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca agcgatcatg
    69541 aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc tcctgctata
    69601 agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta atcacaatca
    69661 aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta acggatcatg
    69721 tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat ggtttagtgg
    69781 gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa
    69841 cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa
    69901 tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt ttgaatccta
    69961 aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc
    70021 aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg gaagatgcgc
    70081 tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga gtagcccgtg
    70141 ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct ctggatctat
    70201 ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac ggcctcagct
    70261 atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat atcgcaatta
    70321 tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat gacccgcgac
    70381 atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa
    70441 gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa aaagaatact
    70501 atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac aaagtggcgt
    70561 tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt tattgatgat
    70621 cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga tgccggggga
    70681 tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac agatgcgaca
    70741 agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg gaaatgcttt
    70801 gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact tgatcggcga
    70861 acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt atttgattgc
    70921 tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata agattgtcgt
    70981 aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga tcatgctatt
    71041 tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac taggtttgca
    71101 aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac catcaattac
    71161 ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag taatcgattc
    71221 gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga ataaagtatt
    71281 cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat
    71341 cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc cgggagtcgg
    71401 aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat ccttagtatt
    71461 gattttagga atcgcgacgc tagtggggac attactttca gatatcatca tgagtatcgt
    71521 tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc gatgagtgaa
    71581 aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat gattgctcga
    71641 gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt tttattatta
    71701 gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt tagtatcttc
    71761 gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat tctgggagca
    71821 gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa ctctgttacg
    71881 atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg gatcatctct
    71941 ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt tatcatgatt
    72001 ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata caatgtctgg
    72061 tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt attccgaagc
    72121 aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat gggaaccaat
    72181 gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat cattactaat
    72241 ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt cttaggtttc
    72301 ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag taacggagaa
    72361 gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt agttttgatg
    72421 ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg ccaacggtta
    72481 ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg gatttgcagc
    72541 gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata cctctggaaa
    72601 caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg caacaaaaaa
    72661 taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt
    72721 taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt ttatggctcc
    72781 ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg acggagctgc
    72841 aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa
    72901 atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg aaatcattgg
    72961 gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg taggtatgga
    73021 agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag acgacaaaac
    73081 agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg gtggtatctg
    73141 gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata tggaatcaag
    73201 cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta atatcgttgc
    73261 aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc ctaaattgaa
    73321 aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga actctaaatt
    73381 atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag aaattgctgg
    73441 ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca agttaggtaa
    73501 atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg ctgataaatc
    73561 attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac gtttctatgc
    73621 aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat accatgatga
    73681 tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag atgatgctgg
    73741 ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata aactaacaat
    73801 caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt actatatcca
    73861 acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga tcgaattcaa
    73921 ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc aagctgcttg
    73981 gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg cgttcaacta
    74041 cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt caagcaaagc
    74101 atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg catttgaaga
    74161 agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa
    74221 agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg ttacagcaga
    74281 ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt cggttttttg
    74341 atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat gacttctcca
    74401 ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca aaaatatgga
    74461 aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt tcacaacttc
    74521 tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca tttcaaattt
    74581 gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa
    74641 atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt tcacgatcta
    74701 aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg caatctgcaa
    74761 ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact gatggtttct
    74821 cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa aacacgagct
    74881 gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat
    74941 gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc attgctttgc
    75001 gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga cacgacccat
    75061 gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc acatgagatg
    75121 gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc aatcgaatac
    75181 cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga agtttatgta
    75241 gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga acaagctgcg
    75301 gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt atttgaaacg
    75361 aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt ttgaaaatag
    75421 cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag aggctatccg
    75481 ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat
    75541 ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag tgattttaga
    75601 caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc gccgttatcg
    75661 acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa aagatatcca
    75721 agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa
    75781 agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg aagaagcagc
    75841 tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga
    75901 agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt taactccttt
    75961 ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac tgactcaaac
    76021 cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca
    76081 agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga tccaagaaca
    76141 agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa
    76201 aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag gacaaaaaga
    76261 tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc aaacttcatt
    76321 agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct tgatgaaagc
    76381 agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa aaacacagca
    76441 agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa gagatcaata
    76501 tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca
    76561 atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg aagtagaagc
    76621 atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg cgcagctaaa
    76681 atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt
    76741 ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc aagtccagca
    76801 gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg gtttttacca
    76861 aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg gagcagtggc
    76921 tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt taggtggggc
    76981 agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca cgtatttgaa
    77041 acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac caagacaatt
    77101 accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga ttgccagtga
    77161 acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag gaacgatcct
    77221 tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg
    77281 agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag gtggagcgaa
    77341 taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac agttgactag
    77401 tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc aggagctgca
    77461 acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag gcgaacaatt
    77521 acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg aacttgaacg
    77581 ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc agtttatcga
    77641 agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg aatcgcaacg
    77701 acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag
    77761 aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg tcttgaaaga
    77821 gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag cagaggcaac
    77881 cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact tttctgatca
    77941 tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc agcgagaaac
    78001 attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa aggagatcga
    78061 taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca
    78121 atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc atttgaccta
    78181 tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc aagaaacaac
    78241 cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg aaaaactggg
    78301 accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac atacattctt
    78361 agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga
    78421 gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc gtcaagaatt
    78481 caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac tgacggaacc
    78541 aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca aaaaactgca
    78601 aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt tgctcttttc
    78661 cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag cagcgttaga
    78721 tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg acacacaatt
    78781 tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt acggagtgac
    78841 gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata tcaatgaaga
    78901 ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt agcaatcgat
    78961 ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa acaagcatta
    79021 gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc cttagctgcg
    79081 atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag catcactttc
    79141 aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa agtgatggaa
    79201 gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc actagatgtt
    79261 ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc cttgtacaat
    79321 gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac agatgaattt
    79381 gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa aatcaaagaa
    79441 ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat gcttttggaa
    79501 tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa agatcttggt
    79561 ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc gatgatccaa
    79621 tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga agcagcagat
    79681 ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa atttgtatta
    79741 gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta ctggcgaaga
    79801 gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa
    79861 aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg atgaagactt
    79921 ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa cagcgatacg
    79981 aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt
    80041 ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt
    80101 aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg gtgttaatgg
    80161 tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa
    80221 agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt tagttgtctg
    80281 gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc cagcagctgt
    80341 cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac tagttgatac
    80401 tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt
    80461 aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag atgcaaccac
    80521 tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg tgaccggtct
    80581 tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta tccgtaatga
    80641 attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact tggagccgtt
    80701 tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag aataacccat
    80761 gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa
    80821 catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt ctcaaacaat
    80881 ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg gaggtcactt
    80941 aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta tcgaccaagc
    81001 tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac
    81061 cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa cagattttag
    81121 cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat tatttcagta
    81181 tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga tgatctggcc
    81241 attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg caaaattaaa
    81301 aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga agcgaaaaca
    81361 gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa gaacattgtt
    81421 cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt atttgaaaac
    81481 agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg cttcaccaat
    81541 gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc gcagtattcg
    81601 caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa
    81661 acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg aggagaaaga
    81721 attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag caacaacagg
    81781 tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat tgggtatcaa
    81841 tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga cagaagaaaa
    81901 agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag tagcactaga
    81961 acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg aaggaatgaa
    82021 gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg aagcattgac
    82081 tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag cacagctatc
    82141 gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa gccatgaacg
    82201 tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat ttatgttcga
    82261 tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat ttttgcgtct
    82321 gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta aggacagcta
    82381 tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg
    82441 gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa aagcttatcc
    82501 gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg ggttaggtat
    82561 cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag ccattgcttt
    82621 tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat atcgtttctt
    82681 tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg ttattggtga
    82741 cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt tgcgtggaac
    82801 gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat tgatgttgaa
    82861 agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt atctaaggga
    82921 gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat tttctgaagc
    82981 ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta gtggtatcga
    83041 tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat atgggatcat
    83101 cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag gaaaaggcag
    83161 aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa agaaaaagct
    83221 tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat
    83281 ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt ttttggtttt
    83341 tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc aatcacagat
    83401 tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt tgcagtgacg
    83461 taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat atattggttt
    83521 tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg ttttttgagt
    83581 tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt catactaaga
    83641 tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt cactaaaggt
    83701 gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat aaaaaatacc
    83761 ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata cctaaaacat
    83821 atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa aatcattctc
    83881 aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag ttattttaag
    83941 tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc tccagttaga
    84001 tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt gtttgatcct
    84061 tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc aaaataaatt
    84121 tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt tagtggctat
    84181 gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt gctttgcttt
    84241 tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa atcaattgat
    84301 gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta ctttttttgc
    84361 ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt ttgcgactgt
    84421 gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct tggtttggct
    84481 atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa atctgggaat
    84541 agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta gtgcagtcat
    84601 gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc ttgtctctag
    84661 aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag gtagtttgtt
    84721 cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa taccagtcag
    84781 tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa ggggacgacg
    84841 atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt ttatttgtta
    84901 caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt gcattgagga
    84961 gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc acaatcagct
    85021 ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt gagtcgttat
    85081 atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg cttcagcaga
    85141 caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt ctattgttat
    85201 ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg tcagggatga
    85261 ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg gatccgaatg
    85321 aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag agtcagtatc
    85381 ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga tatcgacatg
    85441 tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata gacacagatc
    85501 gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca acggctcttg
    85561 tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt gtcttttcta
    85621 cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg tttctgacag
    85681 gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt gtcgtcgttg
    85741 aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag agtatcctat
    85801 gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta aagatgtaca
    85861 gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg ctggaaagtc
    85921 aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa tctatttaga
    85981 ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat cgatcctacg
    86041 acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt
    86101 tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg aatcactgga
    86161 acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag gcggtcaact
    86221 ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt tactggatga
    86281 acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt taaaaaattt
    86341 agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact ttgctgcaag
    86401 ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag gaacgaccca
    86461 agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac ggatttgtga
    86521 gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga gaagagatga
    86581 aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca tgtggaaata
    86641 gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa actacattga
    86701 cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg
    86761 tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat aaggtagttg
    86821 gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta gaatcagcag
    86881 gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat ctaatcatca
    86941 tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg accattttcc
    87001 tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact gctttagggg
    87061 agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct agtgcaatcg
    87121 atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca cttgtgaatg
    87181 aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat gatttatttg
    87241 gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt gtttcttatg
    87301 aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca aaagctattg
    87361 gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa acgaatgctg
    87421 gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc gggggcggtt
    87481 tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag cataaataac
    87541 agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta aatagcaaga
    87601 gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg attttacgga
    87661 tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta catcatacgg
    87721 gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca gcatcaagat
    87781 cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact taaatttaga
    87841 tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc ttctcggcaa
    87901 taag
//
LOCUS       DASJOX010000002        68250 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJOX010000002 DASJOX010000000
VERSION     DASJOX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.002"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..1168
                     /locus_tag="U4A12_000098"
     CDS             104..1168
                     /locus_tag="U4A12_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1772515.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1414..1839
                     /locus_tag="U4A12_000099"
     CDS             1414..1839
                     /locus_tag="U4A12_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1772516.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1832..2281
                     /gene="nusB"
                     /locus_tag="U4A12_000100"
     CDS             1832..2281
                     /gene="nusB"
                     /locus_tag="U4A12_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN1772517.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2378..3232
                     /locus_tag="U4A12_000101"
     CDS             2378..3232
                     /locus_tag="U4A12_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN1772518.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3233..4570
                     /locus_tag="U4A12_000102"
     CDS             3233..4570
                     /locus_tag="U4A12_000102"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN1772519.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4575..4799
                     /locus_tag="U4A12_000103"
     CDS             4575..4799
                     /locus_tag="U4A12_000103"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN1772520.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4801..5691
                     /locus_tag="U4A12_000104"
     CDS             4801..5691
                     /locus_tag="U4A12_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1772521.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5700..6518
                     /locus_tag="U4A12_000105"
     CDS             5700..6518
                     /locus_tag="U4A12_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN1772522.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6625..7074
                     /locus_tag="U4A12_000106"
     CDS             6625..7074
                     /locus_tag="U4A12_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1772523.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7092..8774
                     /gene="recN"
                     /locus_tag="U4A12_000107"
     CDS             7092..8774
                     /gene="recN"
                     /locus_tag="U4A12_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN1772524.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8829..8945)
                     /locus_tag="U4A12_000108"
     CDS             complement(8829..8945)
                     /locus_tag="U4A12_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN1772525.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9018..9974)
                     /locus_tag="U4A12_000109"
     CDS             complement(9018..9974)
                     /locus_tag="U4A12_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1772526.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10145..10513
                     /locus_tag="U4A12_000110"
     CDS             10145..10513
                     /locus_tag="U4A12_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN1772527.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10683..11114
                     /gene="mraZ"
                     /locus_tag="U4A12_000111"
     CDS             10683..11114
                     /gene="mraZ"
                     /locus_tag="U4A12_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN1772528.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11135..12094
                     /gene="rsmH"
                     /locus_tag="U4A12_000112"
     CDS             11135..12094
                     /gene="rsmH"
                     /locus_tag="U4A12_000112"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN1772529.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12099..12500
                     /gene="ftsL"
                     /locus_tag="U4A12_000113"
     CDS             12099..12500
                     /gene="ftsL"
                     /locus_tag="U4A12_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN1772530.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12501..14693
                     /locus_tag="U4A12_000114"
     CDS             12501..14693
                     /locus_tag="U4A12_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1772531.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14719..15681
                     /locus_tag="U4A12_000115"
     CDS             14719..15681
                     /locus_tag="U4A12_000115"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN1772532.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15695..17056
                     /locus_tag="U4A12_000116"
     CDS             15695..17056
                     /locus_tag="U4A12_000116"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN1772533.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17082..18170
                     /gene="murG"
                     /locus_tag="U4A12_000117"
     CDS             17082..18170
                     /gene="murG"
                     /locus_tag="U4A12_000117"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN1772534.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18185..19405
                     /locus_tag="U4A12_000118"
     CDS             18185..19405
                     /locus_tag="U4A12_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN1772535.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19561..20886
                     /gene="ftsA"
                     /locus_tag="U4A12_000119"
     CDS             19561..20886
                     /gene="ftsA"
                     /locus_tag="U4A12_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN1772536.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20909..22150
                     /gene="ftsZ"
                     /locus_tag="U4A12_000120"
     CDS             20909..22150
                     /gene="ftsZ"
                     /locus_tag="U4A12_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN1772537.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22167..22844
                     /locus_tag="U4A12_000121"
     CDS             22167..22844
                     /locus_tag="U4A12_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN1772538.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22858..23457
                     /locus_tag="U4A12_000122"
     CDS             22858..23457
                     /locus_tag="U4A12_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN1772539.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23472..23759
                     /locus_tag="U4A12_000123"
     CDS             23472..23759
                     /locus_tag="U4A12_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN1772540.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23808..24590
                     /locus_tag="U4A12_000124"
     CDS             23808..24590
                     /locus_tag="U4A12_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN1772541.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24611..25318
                     /locus_tag="U4A12_000125"
     CDS             24611..25318
                     /locus_tag="U4A12_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN1772542.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25580..28378
                     /gene="ileS"
                     /locus_tag="U4A12_000126"
     CDS             25580..28378
                     /gene="ileS"
                     /locus_tag="U4A12_000126"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN1772543.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28427..29953)
                     /locus_tag="U4A12_000127"
     CDS             complement(28427..29953)
                     /locus_tag="U4A12_000127"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN1772544.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29968..30615)
                     /locus_tag="U4A12_000128"
     CDS             complement(29968..30615)
                     /locus_tag="U4A12_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN1772545.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30799..31128)
                     /locus_tag="U4A12_000129"
     CDS             complement(30799..31128)
                     /locus_tag="U4A12_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN1772546.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31305..32033
                     /gene="sfsA"
                     /locus_tag="U4A12_000130"
     CDS             31305..32033
                     /gene="sfsA"
                     /locus_tag="U4A12_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN1772547.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32049..33062
                     /locus_tag="U4A12_000131"
     CDS             32049..33062
                     /locus_tag="U4A12_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1772548.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33062..34339
                     /locus_tag="U4A12_000132"
     CDS             33062..34339
                     /locus_tag="U4A12_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1772549.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34402..37104
                     /locus_tag="U4A12_000133"
     CDS             34402..37104
                     /locus_tag="U4A12_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1772550.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37256..37573
                     /locus_tag="U4A12_000134"
     CDS             37256..37573
                     /locus_tag="U4A12_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1772551.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37603..37923
                     /locus_tag="U4A12_000135"
     CDS             37603..37923
                     /locus_tag="U4A12_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1772552.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38031..39491
                     /locus_tag="U4A12_000136"
     CDS             38031..39491
                     /locus_tag="U4A12_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1772553.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39559..39780
                     /locus_tag="U4A12_000137"
     CDS             39559..39780
                     /locus_tag="U4A12_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772554.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39811..39993
                     /locus_tag="U4A12_000138"
     CDS             39811..39993
                     /locus_tag="U4A12_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN1772555.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39993..40406
                     /locus_tag="U4A12_000139"
     CDS             39993..40406
                     /locus_tag="U4A12_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN1772556.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40529..41710
                     /locus_tag="U4A12_000140"
     CDS             40529..41710
                     /locus_tag="U4A12_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1772557.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41781..41945)
                     /locus_tag="U4A12_000141"
     CDS             complement(41781..41945)
                     /locus_tag="U4A12_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772558.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42099..42172
                     /locus_tag="U4A12_000142"
     tRNA            42099..42172
                     /locus_tag="U4A12_000142"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42133..42135,aa:Arg,seq:cct)
     gene            complement(42209..42409)
                     /locus_tag="U4A12_000143"
     CDS             complement(42209..42409)
                     /locus_tag="U4A12_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1772559.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(42714..43946)
                     /locus_tag="U4A12_000144"
     CDS             complement(42714..43946)
                     /locus_tag="U4A12_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN1772560.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            44200..44769
                     /locus_tag="U4A12_000145"
     CDS             44200..44769
                     /locus_tag="U4A12_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1772561.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(44947..45387)
                     /locus_tag="U4A12_000146"
     CDS             complement(44947..45387)
                     /locus_tag="U4A12_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN1772562.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            45545..46309
                     /gene="map"
                     /locus_tag="U4A12_000147"
     CDS             45545..46309
                     /gene="map"
                     /locus_tag="U4A12_000147"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN1772563.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            46341..47264
                     /locus_tag="U4A12_000148"
     CDS             46341..47264
                     /locus_tag="U4A12_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN1772564.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            47340..48479
                     /locus_tag="U4A12_000149"
     CDS             47340..48479
                     /locus_tag="U4A12_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN1772565.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            48472..49272
                     /locus_tag="U4A12_000150"
     CDS             48472..49272
                     /locus_tag="U4A12_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1772566.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            49272..50099
                     /locus_tag="U4A12_000151"
     CDS             49272..50099
                     /locus_tag="U4A12_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1772567.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            50077..50811
                     /locus_tag="U4A12_000152"
     CDS             50077..50811
                     /locus_tag="U4A12_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1772568.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            50911..51777
                     /gene="rfbA"
                     /locus_tag="U4A12_000153"
     CDS             50911..51777
                     /gene="rfbA"
                     /locus_tag="U4A12_000153"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN1772569.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            51791..52363
                     /gene="rfbC"
                     /locus_tag="U4A12_000154"
     CDS             51791..52363
                     /gene="rfbC"
                     /locus_tag="U4A12_000154"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN1772570.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            52385..53413
                     /gene="rfbB"
                     /locus_tag="U4A12_000155"
     CDS             52385..53413
                     /gene="rfbB"
                     /locus_tag="U4A12_000155"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN1772571.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            53511..54362
                     /gene="rfbD"
                     /locus_tag="U4A12_000156"
     CDS             53511..54362
                     /gene="rfbD"
                     /locus_tag="U4A12_000156"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN1772572.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            54397..56430
                     /locus_tag="U4A12_000157"
     CDS             54397..56430
                     /locus_tag="U4A12_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772573.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            56474..57754
                     /locus_tag="U4A12_000158"
     CDS             56474..57754
                     /locus_tag="U4A12_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN1772574.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            57964..58770
                     /locus_tag="U4A12_000159"
     CDS             57964..58770
                     /locus_tag="U4A12_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772575.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            58782..60002
                     /locus_tag="U4A12_000160"
     CDS             58782..60002
                     /locus_tag="U4A12_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772576.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            59992..61578
                     /locus_tag="U4A12_000161"
     CDS             59992..61578
                     /locus_tag="U4A12_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1772577.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            61617..63755
                     /locus_tag="U4A12_000162"
     CDS             61617..63755
                     /locus_tag="U4A12_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1772578.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(64123..65106)
                     /locus_tag="U4A12_000163"
     CDS             complement(64123..65106)
                     /locus_tag="U4A12_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1772579.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            65484..66875
                     /locus_tag="U4A12_000164"
     CDS             65484..66875
                     /locus_tag="U4A12_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1772580.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            66891..67931
                     /locus_tag="U4A12_000165"
     CDS             66891..67931
                     /locus_tag="U4A12_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1772581.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            67950..>68250
                     /locus_tag="U4A12_000166"
     CDS             67950..>68250
                     /locus_tag="U4A12_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1772582.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINL"
BASE COUNT        22382 a        11561 c        14392 g        19915 t
ORIGIN      
        1 taaaataaaa agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt
       61 gctataatgg aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga
      121 aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta
      181 caatcttcgt tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga
      241 aaaagcattt tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg
      301 gtttgaaatt attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa
      361 agaagaactg cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt
      421 tttagaagaa atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag
      481 agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat
      541 ggcatatgat cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa
      601 tcaactagac ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt
      661 tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca
      721 aggtgacttg atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac
      781 acgtacattc gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt
      841 agatgctcaa ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga
      901 tgcagttgct agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac
      961 aggacatggg atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa
     1021 gcaatttgta ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg
     1081 tggagtacgt attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg
     1141 ttcaccaaaa gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc
     1201 agtgttcaca gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact
     1261 tttaagctgt aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct
     1321 gatcggcggc agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt
     1381 atctcgtgct taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta
     1441 aacgcaaacc aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc
     1501 gggattgcag cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat
     1561 gtcactgaat tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa
     1621 gatggcttga aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta
     1681 gcaatggaaa tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta
     1741 tctgaagtca atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc
     1801 gaagaacttt ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat
     1861 tcgggaaatg gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga
     1921 agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc
     1981 agagtttgtt cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga
     2041 attggataaa gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa
     2101 aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc
     2161 agcggctgtt gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc
     2221 acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta
     2281 agtcctagct tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg
     2341 tagaatgaaa acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa
     2401 agaattagct gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa
     2461 tggtatccat cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta
     2521 tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta
     2581 tcctgagacg atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc
     2641 tagttttcat ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat
     2701 cctgttagct atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag
     2761 actccttgcg ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt
     2821 tgaagcttat tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat
     2881 cgtcggtaaa ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca
     2941 ttctaaaacc gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat
     3001 aggacgtgga cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt
     3061 agggatgaat cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga
     3121 gcctattgca ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat
     3181 gctgatgtat caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca
     3241 agaatattta actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc
     3301 ttatttagaa cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc
     3361 ccatcaatat ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg
     3421 agcattccaa aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg
     3481 tatttcatta tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga
     3541 tggaataggt gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg
     3601 cctttttcaa gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac
     3661 cagtccaagc ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat
     3721 tgtccaattg gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt
     3781 caggaatatc cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg
     3841 gggtggttcg attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta
     3901 tcaagctaat acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga
     3961 tttagtagca gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt
     4021 tttgtcagaa gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt
     4081 acgccagatc cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg
     4141 gcagcctgaa agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt
     4201 atatcaagct acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca
     4261 ccaattggaa caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta
     4321 tctagaaaaa cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt
     4381 tgctcaagct gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta
     4441 cagctatacg acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga
     4501 tttgctgacg atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa
     4561 aggagaataa aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa
     4621 ttgtcatgca gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac
     4681 gaggaatgga attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga
     4741 cgaaaatgat gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag
     4801 atgagattaa ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct
     4861 tttttagatg agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa
     4921 gcaggcggga aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag
     4981 cctattgatg ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc
     5041 ttgatccacg atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc
     5101 aatcataaag tattcggcga agcattagct atattggccg gagatggttt gcttacagga
     5161 gcattccagc taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag
     5221 caattagctg tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag
     5281 ggcgaatcga aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg
     5341 catttgattc gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc
     5401 ttattgctac ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat
     5461 ctactcgatg tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt
     5521 atggaaaaaa atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag
     5581 attgagctgt tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt
     5641 gatggcgggc tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa
     5701 tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac
     5761 aagccaaacg ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata
     5821 aaccgggaga aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt
     5881 atgtttctcg agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg
     5941 aaggaaaaac attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc
     6001 aaaatggcgc aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa
     6061 taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg
     6121 aagactttac cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa
     6181 aactgatgct gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa
     6241 tcaaacccca attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc
     6301 ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg
     6361 atgtgctgga attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg
     6421 ctcacttgag gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag
     6481 tggtttcaaa tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt
     6541 gagaagtatg cggattcttc tttattatac caaggaaata agctatcatt gattaagaag
     6601 aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga
     6661 ttgttgagtg agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa
     6721 ggaatatctg ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag
     6781 gtaccggctt cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta
     6841 aatactaaat tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa
     6901 tttgttattt tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa
     6961 cgttacaaga aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca
     7021 cgaactgaag accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag
     7081 gagcgaatgt gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc
     7141 ggctttcttt tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca
     7201 ttattatcga tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc
     7261 aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct
     7321 ctgaactgat ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg
     7381 atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta
     7441 atctgagaag aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac
     7501 tgctccagcc tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac
     7561 agaagaaaaa gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac
     7621 gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc
     7681 aagaagaaat agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa
     7741 gagaaaaact tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac
     7801 tgacgaataa tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag
     7861 ggatcgccca tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact
     7921 atttgctgca agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg
     7981 agagtcgttt agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat
     8041 acggcgaatc cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt
     8101 cttctgattt ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc
     8161 ttctacttca gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat
     8221 tggaaaaatc cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg
     8281 tacgtttctt aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat
     8341 tttacattac gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg
     8401 gcgaactgtc tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga
     8461 caagtattat ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg
     8521 cagataaaat ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc
     8581 aagttgctgc agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta
     8641 cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc
     8701 tggctggaag tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg
     8761 caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata
     8821 ttttattttt acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc
     8881 agcattgtcc aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca
     8941 cgcattgttt tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt
     9001 tccacaagag ggctttttta taaaaacttc ccttttttta aataataggt agttacgata
     9061 caaataaata atgttaatag aactagccat ataaaggcat catccctttc agcaataggt
     9121 aacttaatgt tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt
     9181 aaagaagtta aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc
     9241 tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt
     9301 tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt
     9361 tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg
     9421 cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg
     9481 gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg
     9541 acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag
     9601 gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc
     9661 tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga
     9721 cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga
     9781 ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac
     9841 ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa
     9901 taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta
     9961 aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa
    10021 ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa
    10081 tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac
    10141 gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt
    10201 gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc
    10261 ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg
    10321 gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc
    10381 tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg
    10441 gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact
    10501 ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc
    10561 ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg
    10621 tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt
    10681 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
    10741 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
    10801 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
    10861 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
    10921 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
    10981 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
    11041 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
    11101 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
    11161 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
    11221 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
    11281 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
    11341 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
    11401 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
    11461 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
    11521 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
    11581 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
    11641 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
    11701 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
    11761 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
    11821 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
    11881 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
    11941 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
    12001 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
    12061 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
    12121 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
    12181 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
    12241 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
    12301 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
    12361 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
    12421 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
    12481 atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
    12541 aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
    12601 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
    12661 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
    12721 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
    12781 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
    12841 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
    12901 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
    12961 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
    13021 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
    13081 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
    13141 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
    13201 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
    13261 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
    13321 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
    13381 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
    13441 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
    13501 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
    13561 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
    13621 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
    13681 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
    13741 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
    13801 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
    13861 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
    13921 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
    13981 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
    14041 tcgaaagcga aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat
    14101 cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata
    14161 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
    14221 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
    14281 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
    14341 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
    14401 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
    14461 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
    14521 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
    14581 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
    14641 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
    14701 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
    14761 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
    14821 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
    14881 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
    14941 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
    15001 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
    15061 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
    15121 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
    15181 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
    15241 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
    15301 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
    15361 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
    15421 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
    15481 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
    15541 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
    15601 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
    15661 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
    15721 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
    15781 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
    15841 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
    15901 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
    15961 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
    16021 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
    16081 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
    16141 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
    16201 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
    16261 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
    16321 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
    16381 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
    16441 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
    16501 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
    16561 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
    16621 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
    16681 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
    16741 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
    16801 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
    16861 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
    16921 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
    16981 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
    17041 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
    17101 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
    17161 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
    17221 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
    17281 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
    17341 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
    17401 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
    17461 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
    17521 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
    17581 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
    17641 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
    17701 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
    17761 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
    17821 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
    17881 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
    17941 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
    18001 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
    18061 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
    18121 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
    18181 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
    18241 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
    18301 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
    18361 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
    18421 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
    18481 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
    18541 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
    18601 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
    18661 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
    18721 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
    18781 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
    18841 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
    18901 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
    18961 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
    19021 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
    19081 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
    19141 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
    19201 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
    19261 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
    19321 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
    19381 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
    19441 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
    19501 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
    19561 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
    19621 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
    19681 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
    19741 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
    19801 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
    19861 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
    19921 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
    19981 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
    20041 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
    20101 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
    20161 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
    20221 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
    20281 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
    20341 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
    20401 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
    20461 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
    20521 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
    20581 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    20641 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    20701 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    20761 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    20821 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    20881 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    20941 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    21001 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    21061 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    21121 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    21181 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    21241 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    21301 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    21361 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    21421 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    21481 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    21541 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    21601 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    21661 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    21721 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    21781 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    21841 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    21901 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    21961 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    22021 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    22081 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    22141 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    22201 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    22261 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    22321 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    22381 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    22441 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    22501 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    22561 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    22621 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    22681 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    22741 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    22801 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    22861 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    22921 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    22981 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    23041 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    23101 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    23161 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    23221 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    23281 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    23341 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    23401 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    23461 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    23521 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    23581 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    23641 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    23701 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    23761 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    23821 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg
    23881 aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct
    23941 atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat
    24001 atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg
    24061 aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc
    24121 atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata
    24181 tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg
    24241 ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag
    24301 aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac
    24361 tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg
    24421 aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac
    24481 tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga
    24541 agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg
    24601 tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat
    24661 gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga
    24721 ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact
    24781 tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac
    24841 ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca
    24901 aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga
    24961 tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc
    25021 gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat
    25081 gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt
    25141 ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga
    25201 caatgaaaat gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc
    25261 atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa
    25321 cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac
    25381 tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc
    25441 ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt
    25501 ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa
    25561 taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt
    25621 ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa
    25681 tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga
    25741 tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa
    25801 agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg
    25861 ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga
    25921 aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa
    25981 gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac
    26041 attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg
    26101 ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc
    26161 ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt
    26221 caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc
    26281 atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt
    26341 ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga
    26401 aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat
    26461 gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac
    26521 tttagacgct ggtactggat tagtccatac tgctccagga catggggaag atgactatat
    26581 cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac
    26641 agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga
    26701 tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca
    26761 cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat
    26821 cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg
    26881 gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca
    26941 gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac
    27001 accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt
    27061 tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg
    27121 cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga
    27181 agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc
    27241 agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt
    27301 agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat
    27361 gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga
    27421 tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga
    27481 tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt
    27541 agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc
    27601 tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg
    27661 gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga
    27721 tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga
    27781 ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt
    27841 attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga
    27901 ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt
    27961 gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga
    28021 agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa
    28081 acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt
    28141 ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt
    28201 atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg
    28261 ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc
    28321 aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa
    28381 gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt
    28441 ttccacgttc acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa
    28501 gtagttcgaa agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt
    28561 gtgtttggtc ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt
    28621 cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag
    28681 cttctgggct attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg
    28741 tttcaaatcc ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct
    28801 gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag
    28861 atgttttgaa tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt
    28921 cagtcaaacg tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga
    28981 tagtgaattt gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt
    29041 cgcgatagcc cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga
    29101 agttttctaa cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt
    29161 ctgtacggat ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa
    29221 cctgtaaaat atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc
    29281 cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat
    29341 tgttccattg agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca
    29401 tttcttttcc taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga
    29461 tttgctggtt caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca
    29521 aacgattgaa gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc
    29581 caaaaaattg aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc
    29641 catctaatcg gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat
    29701 gggattgata gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg
    29761 cacggattgt ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg
    29821 ttccgattac agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg
    29881 ggtacaattt acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt
    29941 tttcattaga catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc
    30001 cttaaagctg acggggattt tctttctatc attttggata gtgatcggtc cttcataagc
    30061 tgcgatgtca acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc
    30121 caacaattct ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga
    30181 ctccaatgtt tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc
    30241 atgaggacaa tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga
    30301 agtcacatgt tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg
    30361 ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag
    30421 tccgcttgca gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac
    30481 aagtttagaa atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg
    30541 tttattgttg actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc
    30601 tcgatttggt gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta
    30661 acaaaaaaaa cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt
    30721 ttacaacaaa aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat
    30781 atatacattg ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc
    30841 gtcataaaga gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac
    30901 gactaaacga tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa
    30961 aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca
    31021 tgtaccttga tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta
    31081 aataaaactt gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac
    31141 caaatcactc ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt
    31201 gtaccgaata aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct
    31261 acaaattatg ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag
    31321 tggaaatagc tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag
    31381 acggagaaat aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac
    31441 caggcgctga agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc
    31501 tgattgctgt aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc
    31561 tggcaattga tggtatttta gacggcacga tccttttacc aggattaact ggtccattta
    31621 cggtatacaa gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg
    31681 tggtcggaca aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg
    31741 gtgcttttcc tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg
    31801 ccgcacaaga tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc
    31861 tggcaacgat ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc
    31921 aaagcggagt tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca
    31981 agcaggccat tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag
    32041 gtaaaataat gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc
    32101 aagcagcttt aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa
    32161 aagcacagga atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga
    32221 cttttttcaa tattactcat atggacacca tttatattgc ttctccgaat agtcttcact
    32281 ttagtcaagc taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt
    32341 tttcgacgcc aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt
    32401 tttttgaagc agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac
    32461 cattaaaaga cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg
    32521 accaagtatt agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat
    32581 tgatggattt aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag
    32641 aagtccatta tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct
    32701 tacgttatga gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac
    32761 cgtcagagat ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac
    32821 aagcacaatt tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag
    32881 aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc
    32941 cagcttgggg attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga
    33001 tttatgattt aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt
    33061 aatgaacgaa tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg
    33121 gtttatctca ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag
    33181 tatagtagcg gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact
    33241 actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact
    33301 agctattcaa gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca
    33361 atctttggtg ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga
    33421 aatcttgatt ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc
    33481 acatcaagtg aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag
    33541 ccaaatcatt gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc
    33601 agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac
    33661 aattgacgtg acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt
    33721 accgatacga aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg
    33781 tctgattttc tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag
    33841 aggtgtaccg gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct
    33901 agaacaattc aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg
    33961 tttagatatt acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc
    34021 ttatttgcat cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact
    34081 cgtacaagat aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact
    34141 tgaagagatt tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc
    34201 agctcctgca gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga
    34261 atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa
    34321 aggagcaagg cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt
    34381 ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca
    34441 gaaaaccaac aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc
    34501 aatgcaagcc gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc
    34561 cgaccagtcg tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac
    34621 tcttctacgc aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc
    34681 atacagcagg caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt
    34741 ggagaaactg gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat
    34801 tcagagatgc tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa
    34861 aatccgcaac tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca
    34921 gctcaagata gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag
    34981 atccaccgtt taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt
    35041 taccgtccgc ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg
    35101 acgactgaat cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc
    35161 accttgccga atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt
    35221 atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt
    35281 gccttcatgc tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta
    35341 gtctgtgcaa aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga
    35401 aaagaagaac tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa
    35461 cgtctcgacc gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct
    35521 gatgttgtct ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa
    35581 aaagtcactt ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca
    35641 aaagatgtgg atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc
    35701 aaagatcttt tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa
    35761 aaacggttag accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa
    35821 agtacattgg atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta
    35881 cggaaaaatt ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa
    35941 gaagaacaag ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc
    36001 aatctaggag aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac
    36061 ggcagacaga ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta
    36121 gggaaagctt ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta
    36181 caatatgtca aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg
    36241 ggatcattaa acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct
    36301 atcacgatga caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga
    36361 agcttagaag atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag
    36421 ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt
    36481 actggtgaag gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat
    36541 aaggtagaac tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat
    36601 gccttattgg aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa
    36661 aatatcccat ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag
    36721 cgtatagctg atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta
    36781 agcgatgtct tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc
    36841 cagaccgatt tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta
    36901 gccttcatgt tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct
    36961 attttctcaa aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta
    37021 gaacgaaaat atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt
    37081 cttgaaaata aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg
    37141 tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta
    37201 ttggcatgct tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc
    37261 aaaaaaaaca atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa
    37321 aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc
    37381 agatgcagat aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt
    37441 acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt
    37501 catcaatatg caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa
    37561 tctaatgaaa taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat
    37621 ctagaagcca tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa
    37681 gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca
    37741 gaagaatcac ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa
    37801 ggaaatcata tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct
    37861 attgcgttta ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa
    37921 taaatacaac ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg
    37981 ttataaggag aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac
    38041 tatcaagttg gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac
    38101 atttagttgc tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag
    38161 tcgctgtaat gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg
    38221 ctgacagttt agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca
    38281 atggttttgt ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg
    38341 ggtataatct tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag
    38401 ctaccttgat tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg
    38461 ttccttcaaa tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg
    38521 gcacactgtc agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt
    38581 attttacagt tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg
    38641 ccaacattac gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag
    38701 ccattttacc agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc
    38761 gtgtagctga tgggcaactg atcattgatt tggtgcaaaa atatattgca gagccattcc
    38821 taggattatc tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct
    38881 ggttctttgg tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac
    38941 aagcacaact aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac
    39001 cagccgtgtt agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg
    39061 atgcttttgc atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga
    39121 ttttctctaa acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct
    39181 tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta
    39241 tcccattctt gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg
    39301 gcctagtcgc tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat
    39361 tcttagcaac aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga
    39421 ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg
    39481 aacaacaata agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat
    39541 tttcgaggag gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa
    39601 gaattgttag aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga
    39661 atcaagctgt tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag
    39721 gaactaatca aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag
    39781 aaaatggttt aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt
    39841 attggttctt tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat
    39901 agtatggtca caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa
    39961 aaaagagaag aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc
    40021 cagcatcgtt tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga
    40081 ccggcaaatc agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc
    40141 aaaataaccg ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc
    40201 gtacatcgac aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt
    40261 cgacctgtga ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga
    40321 atgatatgct tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc
    40381 tagatatgat cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa
    40441 aatgaagtaa gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac
    40501 tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc
    40561 ttcgattgag gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga
    40621 aaggcgagaa cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga
    40681 ggaggatcgg ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca
    40741 agagtaccta atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg
    40801 cgggatcaat cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt
    40861 atcttatctg gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt
    40921 tgataaaatc agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg
    40981 aaaaataaag cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca
    41041 gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt
    41101 caagcaaaaa ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg
    41161 atatatcgtt tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga
    41221 tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac
    41281 atttgaatat tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt
    41341 tgcttctgta atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt
    41401 ggaaaattat ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca
    41461 ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct
    41521 ctcaaaagta gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca
    41581 gctattatta gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac
    41641 aaaagaatat gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga
    41701 ctatttttga taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat
    41761 ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg
    41821 ttttcttttg ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt
    41881 tcccgatttt ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca
    41941 ttcattggaa aggacctccc tacattattt cattctacct ctagtttatc atctgttaag
    42001 gactagtgga aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa
    42061 gtaaaaaaca aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata
    42121 gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtacaaata
    42181 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
    42241 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
    42301 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
    42361 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
    42421 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt
    42481 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
    42541 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
    42601 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
    42661 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
    42721 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
    42781 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
    42841 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
    42901 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
    42961 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
    43021 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
    43081 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
    43141 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
    43201 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
    43261 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
    43321 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
    43381 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
    43441 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
    43501 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
    43561 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
    43621 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
    43681 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
    43741 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
    43801 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
    43861 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
    43921 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
    43981 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
    44041 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
    44101 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
    44161 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
    44221 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
    44281 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
    44341 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
    44401 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
    44461 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
    44521 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
    44581 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
    44641 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
    44701 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
    44761 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
    44821 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
    44881 gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca
    44941 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
    45001 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
    45061 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
    45121 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
    45181 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
    45241 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
    45301 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
    45361 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
    45421 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
    45481 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
    45541 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
    45601 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
    45661 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
    45721 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
    45781 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
    45841 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
    45901 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
    45961 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
    46021 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
    46081 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
    46141 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
    46201 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
    46261 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
    46321 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
    46381 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
    46441 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
    46501 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
    46561 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
    46621 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
    46681 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
    46741 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
    46801 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
    46861 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
    46921 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
    46981 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
    47041 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
    47101 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
    47161 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
    47221 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
    47281 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
    47341 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
    47401 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
    47461 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
    47521 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
    47581 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
    47641 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
    47701 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
    47761 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
    47821 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
    47881 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
    47941 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
    48001 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
    48061 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
    48121 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
    48181 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
    48241 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
    48301 cgatcgtatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
    48361 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
    48421 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
    48481 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
    48541 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
    48601 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
    48661 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
    48721 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
    48781 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
    48841 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
    48901 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
    48961 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
    49021 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
    49081 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
    49141 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
    49201 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
    49261 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
    49321 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
    49381 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
    49441 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
    49501 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
    49561 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
    49621 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
    49681 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
    49741 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
    49801 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
    49861 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
    49921 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
    49981 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
    50041 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
    50101 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
    50161 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
    50221 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
    50281 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
    50341 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
    50401 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
    50461 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
    50521 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
    50581 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
    50641 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
    50701 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
    50761 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
    50821 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
    50881 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
    50941 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
    51001 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
    51061 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
    51121 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
    51181 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
    51241 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
    51301 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
    51361 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
    51421 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
    51481 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
    51541 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
    51601 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
    51661 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
    51721 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
    51781 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
    51841 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
    51901 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    51961 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    52021 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    52081 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    52141 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    52201 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    52261 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    52321 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    52381 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    52441 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    52501 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    52561 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    52621 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    52681 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    52741 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    52801 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    52861 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    52921 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    52981 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    53041 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    53101 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    53161 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
    53221 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
    53281 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    53341 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    53401 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
    53461 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
    53521 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
    53581 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
    53641 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
    53701 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
    53761 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
    53821 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    53881 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    53941 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    54001 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    54061 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    54121 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
    54181 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    54241 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
    54301 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    54361 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
    54421 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
    54481 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
    54541 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
    54601 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
    54661 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
    54721 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
    54781 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
    54841 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
    54901 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
    54961 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct
    55021 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
    55081 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
    55141 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
    55201 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
    55261 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
    55321 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
    55381 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
    55441 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
    55501 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
    55561 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
    55621 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
    55681 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
    55741 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
    55801 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
    55861 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
    55921 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
    55981 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
    56041 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
    56101 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
    56161 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
    56221 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
    56281 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
    56341 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
    56401 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
    56461 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
    56521 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
    56581 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
    56641 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
    56701 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
    56761 tggagactta ctgtttcctt tggttatttt attttgttta gtaattaaat ggatcgagtg
    56821 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
    56881 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
    56941 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
    57001 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
    57061 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
    57121 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
    57181 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
    57241 ggctgtctct gaaaaagtca atttgtttac cgatcgagaa gatatttggc ataaattcta
    57301 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    57361 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    57421 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
    57481 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    57541 gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta tttatatcct
    57601 gttggctatg gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat
    57661 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
    57721 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
    57781 gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga
    57841 aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt
    57901 accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca
    57961 caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg
    58021 ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta
    58081 tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga
    58141 ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg
    58201 attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat
    58261 acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta
    58321 tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat
    58381 ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct
    58441 ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg
    58501 caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct
    58561 aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt
    58621 caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact
    58681 ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga
    58741 ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta
    58801 gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg
    58861 aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga
    58921 atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat
    58981 ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg
    59041 aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct
    59101 atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa
    59161 tggtcgatga tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa
    59221 cttattcaag cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg
    59281 atattttgat tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta
    59341 tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata
    59401 tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg
    59461 aatatggcga tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag
    59521 ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg
    59581 acgtgacgaa agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag
    59641 gagaaaaact atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg
    59701 ttttaggaat tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac
    59761 tagatggtat caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa
    59821 agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa
    59881 cggttccttt ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg
    59941 gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat
    60001 gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa
    60061 ccaggaagta aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc
    60121 ggagcaaaac aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc
    60181 agcgaatttg ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat
    60241 tatgctggac agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat
    60301 cctggaaaaa cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt
    60361 acattcccta acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg
    60421 tgggcttctt atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc
    60481 caaaaagttt ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct
    60541 gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat
    60601 ttcaaaatgc gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca
    60661 gtagcacaaa gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta
    60721 acccagcaaa cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg
    60781 gaaaatgaga cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc
    60841 gcgcaacaac ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt
    60901 atcgattcga atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa
    60961 ttaccagagt tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat
    61021 cgctttatcg gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg
    61081 gctgaagtca caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat
    61141 cttcttgagg aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc
    61201 acattgttgg aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa
    61261 acttcgaaaa aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat
    61321 gcagaaacaa aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc
    61381 ttgtcctata agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga
    61441 gaagaagtca atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga
    61501 atcctttgcg agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca
    61561 tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc
    61621 aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt
    61681 tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag
    61741 aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat
    61801 ataatcttga tgttaagacc caaagcggtt tcaaggtacg tttatcaggg aaaatgagag
    61861 gaaaagccat cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa
    61921 acggtaaata cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac
    61981 tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca
    62041 aattggaatt gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac
    62101 cagatttgaa agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca
    62161 tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac
    62221 tggatcaaac gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag
    62281 ctatccgaaa gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc
    62341 gaaaagaaaa tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag
    62401 aatttatagc actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg
    62461 caaaagtgct aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg
    62521 atgcagatgg caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga
    62581 tgggaaataa ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg
    62641 gcggtttccg taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag
    62701 aacaaatccc tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga
    62761 tcccaggatc aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga
    62821 aagcattgac agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa
    62881 tttctggatt ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca
    62941 ttccaacgaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa
    63001 cgtcttattc aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc
    63061 aagaattatt tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg
    63121 acattccatt caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat
    63181 actttttatt cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg
    63241 tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg
    63301 atacaacgca gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga
    63361 ataactatga tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact
    63421 tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt
    63481 ttgatgaaac attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac
    63541 tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa
    63601 gaggttatga agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc
    63661 aagataaatg gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag
    63721 acggaatcgg cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg
    63781 tgattcgctc gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc
    63841 gactcaaaaa aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa
    63901 attcacgaaa attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct
    63961 tctgctccca ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc
    64021 ccacgctctt attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg
    64081 aaccctagct gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg
    64141 tttaaggtat tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt
    64201 gatggagata accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat
    64261 tgctgaatat atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt
    64321 ttgtaagtct tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg
    64381 tacgatttgt tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag
    64441 atcacttgcc gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa
    64501 ccagacattg gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc
    64561 agcataataa ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa
    64621 atcaacagtt gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac
    64681 agtttgatag ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt
    64741 ttttgtccaa tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg
    64801 tgctttcatt gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt
    64861 cactttatac tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg
    64921 ctctaaagca atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca
    64981 agtatcgtct tttttataac cattccattc atctgtataa acttctaccc catccttata
    65041 tgcgacaatt tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt
    65101 attcataaga tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca
    65161 attttttcga ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag
    65221 atggattctc tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata
    65281 cttcgacttc tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt
    65341 tagagtcctt gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca
    65401 ttacaatttg cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt
    65461 attttgtgat taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga
    65521 tattcattat attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac
    65581 tgaagtttcg tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta
    65641 ttatttcagt gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc
    65701 gaatgatcag tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga
    65761 tcatggtagt gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt
    65821 catttgttct cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt
    65881 tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag
    65941 caatccaaaa ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg
    66001 ataactatta tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt
    66061 ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa
    66121 aattgttttt gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt
    66181 tcgaagatga aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat
    66241 tcaaacgttt gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta
    66301 tgttattcac agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag
    66361 tgcggatcac aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca
    66421 cagccgaagc aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg
    66481 taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga
    66541 aaggcgatat gtcgatcgta ggaccacgtc ctgaacgttc tttcttcgtc gatcagttca
    66601 atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg
    66661 cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt
    66721 atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc
    66781 tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag
    66841 aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc
    66901 cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg
    66961 atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa
    67021 cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga
    67081 ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag
    67141 gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt
    67201 atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc
    67261 gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact
    67321 atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt
    67381 atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta
    67441 aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc
    67501 aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca
    67561 ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata
    67621 ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt
    67681 cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag
    67741 caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt
    67801 cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg
    67861 ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc
    67921 gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa
    67981 tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa
    68041 actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag
    68101 agattgaaag attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta
    68161 aatatttaat agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc
    68221 aggtttattt tggcggggga attaatttat
//
LOCUS       DASJOX010000003        61965 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJOX010000003 DASJOX010000000
VERSION     DASJOX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.003"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..359
                     /locus_tag="U4A12_000167"
     CDS             39..359
                     /locus_tag="U4A12_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN1772583.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(471..1340)
                     /locus_tag="U4A12_000168"
     CDS             complement(471..1340)
                     /locus_tag="U4A12_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1772584.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(1436..2739)
                     /locus_tag="U4A12_000169"
                     /pseudo
     CDS             complement(1436..2739)
                     /locus_tag="U4A12_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2736..3245)
                     /locus_tag="U4A12_000170"
     CDS             complement(2736..3245)
                     /locus_tag="U4A12_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772585.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3420..5555)
                     /locus_tag="U4A12_000171"
     CDS             complement(3420..5555)
                     /locus_tag="U4A12_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1772586.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5638..7293)
                     /locus_tag="U4A12_000172"
     CDS             complement(5638..7293)
                     /locus_tag="U4A12_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1772587.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(7295..7609)
                     /locus_tag="U4A12_000173"
     CDS             complement(7295..7609)
                     /locus_tag="U4A12_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1772588.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7779..8039
                     /locus_tag="U4A12_000174"
     CDS             7779..8039
                     /locus_tag="U4A12_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN1772589.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8053..8367
                     /locus_tag="U4A12_000175"
     CDS             8053..8367
                     /locus_tag="U4A12_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN1772590.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(9097..9651)
                     /locus_tag="U4A12_000176"
     CDS             complement(9097..9651)
                     /locus_tag="U4A12_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN1772591.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9777..11705)
                     /locus_tag="U4A12_000177"
     CDS             complement(9777..11705)
                     /locus_tag="U4A12_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1772592.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(12011..12727)
                     /locus_tag="U4A12_000178"
     CDS             complement(12011..12727)
                     /locus_tag="U4A12_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1772593.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12728..14551)
                     /gene="glpO"
                     /locus_tag="U4A12_000179"
     CDS             complement(12728..14551)
                     /gene="glpO"
                     /locus_tag="U4A12_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN1772594.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14565..16061)
                     /gene="glpK"
                     /locus_tag="U4A12_000180"
     CDS             complement(14565..16061)
                     /gene="glpK"
                     /locus_tag="U4A12_000180"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN1772595.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(16197..17657)
                     /locus_tag="U4A12_000181"
     CDS             complement(16197..17657)
                     /locus_tag="U4A12_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772596.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17647..18942)
                     /locus_tag="U4A12_000182"
     CDS             complement(17647..18942)
                     /locus_tag="U4A12_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1772597.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(19274..19951)
                     /locus_tag="U4A12_000183"
     CDS             complement(19274..19951)
                     /locus_tag="U4A12_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN1772598.1"
                     /translation="MKVLIVLTNVEKYEKIELPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(19981..21072)
                     /locus_tag="U4A12_000184"
     CDS             complement(19981..21072)
                     /locus_tag="U4A12_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN1772599.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(21173..21607)
                     /locus_tag="U4A12_000185"
     CDS             complement(21173..21607)
                     /locus_tag="U4A12_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1772600.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(22191..23648)
                     /gene="ascB"
                     /locus_tag="U4A12_000186"
     CDS             complement(22191..23648)
                     /gene="ascB"
                     /locus_tag="U4A12_000186"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1772601.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23645..25540)
                     /locus_tag="U4A12_000187"
     CDS             complement(23645..25540)
                     /locus_tag="U4A12_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1772602.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25598..27373)
                     /locus_tag="U4A12_000188"
     CDS             complement(25598..27373)
                     /locus_tag="U4A12_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1772603.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27423..28919)
                     /locus_tag="U4A12_000189"
     CDS             complement(27423..28919)
                     /locus_tag="U4A12_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1772604.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(29113..30735)
                     /locus_tag="U4A12_000190"
     CDS             complement(29113..30735)
                     /locus_tag="U4A12_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1772605.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(31184..32497)
                     /locus_tag="U4A12_000191"
     CDS             complement(31184..32497)
                     /locus_tag="U4A12_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1772606.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(32742..32966)
                     /locus_tag="U4A12_000192"
     CDS             complement(32742..32966)
                     /locus_tag="U4A12_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772607.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32986..33408)
                     /locus_tag="U4A12_000193"
     CDS             complement(32986..33408)
                     /locus_tag="U4A12_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1772608.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(33552..34262)
                     /locus_tag="U4A12_000194"
     CDS             complement(33552..34262)
                     /locus_tag="U4A12_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772609.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(34280..34777)
                     /locus_tag="U4A12_000195"
     CDS             complement(34280..34777)
                     /locus_tag="U4A12_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1772610.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(34814..35266)
                     /locus_tag="U4A12_000196"
     CDS             complement(34814..35266)
                     /locus_tag="U4A12_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1772611.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(35401..36006)
                     /locus_tag="U4A12_000197"
     CDS             complement(35401..36006)
                     /locus_tag="U4A12_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1772612.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(36456..37076)
                     /locus_tag="U4A12_000198"
     CDS             complement(36456..37076)
                     /locus_tag="U4A12_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1772613.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(37078..37947)
                     /locus_tag="U4A12_000199"
     CDS             complement(37078..37947)
                     /locus_tag="U4A12_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN1772614.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(37970..38377)
                     /locus_tag="U4A12_000200"
     CDS             complement(37970..38377)
                     /locus_tag="U4A12_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1772615.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(38396..39685)
                     /locus_tag="U4A12_000201"
     CDS             complement(38396..39685)
                     /locus_tag="U4A12_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1772616.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(39712..40014)
                     /locus_tag="U4A12_000202"
     CDS             complement(39712..40014)
                     /locus_tag="U4A12_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1772617.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(40039..42132)
                     /locus_tag="U4A12_000203"
     CDS             complement(40039..42132)
                     /locus_tag="U4A12_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1772618.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(42621..43385)
                     /locus_tag="U4A12_000204"
     CDS             complement(42621..43385)
                     /locus_tag="U4A12_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN1772619.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(43496..44365)
                     /gene="mdcB"
                     /locus_tag="U4A12_000205"
     CDS             complement(43496..44365)
                     /gene="mdcB"
                     /locus_tag="U4A12_000205"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN1772620.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(44390..45466)
                     /locus_tag="U4A12_000206"
     CDS             complement(44390..45466)
                     /locus_tag="U4A12_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN1772621.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(45490..46083)
                     /locus_tag="U4A12_000207"
     CDS             complement(45490..46083)
                     /locus_tag="U4A12_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN1772622.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(46087..47763)
                     /gene="mdcD"
                     /locus_tag="U4A12_000208"
     CDS             complement(46087..47763)
                     /gene="mdcD"
                     /locus_tag="U4A12_000208"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN1772623.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(47753..48052)
                     /locus_tag="U4A12_000209"
     CDS             complement(47753..48052)
                     /locus_tag="U4A12_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN1772624.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(48154..49800)
                     /gene="mdcA"
                     /locus_tag="U4A12_000210"
     CDS             complement(48154..49800)
                     /gene="mdcA"
                     /locus_tag="U4A12_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN1772625.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(49807..50718)
                     /locus_tag="U4A12_000211"
     CDS             complement(49807..50718)
                     /locus_tag="U4A12_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1772626.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            50838..51695
                     /locus_tag="U4A12_000212"
     CDS             50838..51695
                     /locus_tag="U4A12_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1772627.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(51882..52244)
                     /locus_tag="U4A12_000213"
     CDS             complement(51882..52244)
                     /locus_tag="U4A12_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN1772628.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(52433..52615)
                     /locus_tag="U4A12_000214"
     CDS             complement(52433..52615)
                     /locus_tag="U4A12_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772629.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            52590..54029
                     /locus_tag="U4A12_000215"
     CDS             52590..54029
                     /locus_tag="U4A12_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772630.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(54036..56588)
                     /locus_tag="U4A12_000216"
     CDS             complement(54036..56588)
                     /locus_tag="U4A12_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN1772631.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(56702..58033)
                     /locus_tag="U4A12_000217"
     CDS             complement(56702..58033)
                     /locus_tag="U4A12_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1772632.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(57999..59477)
                     /locus_tag="U4A12_000218"
     CDS             complement(57999..59477)
                     /locus_tag="U4A12_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1772633.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(59782..61617)
                     /gene="tdc"
                     /locus_tag="U4A12_000219"
     CDS             complement(59782..61617)
                     /gene="tdc"
                     /locus_tag="U4A12_000219"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1772634.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        17785 a        13208 c        10477 g        20495 t
ORIGIN      
        1 agtttgttat actttaatta aaagagagga gtgatcgaat gattacaaca acaacgaaca
       61 gtgtagaggg aaaagccgta gtagcttata aagggatcgt ttttggtgag gtcattactg
      121 ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa
      181 aaagctatga aaacgaacta atgggtgcaa gagaagaagc acttgccgaa atggccgaac
      241 gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa aatggactat gaagtattag
      301 gcagcgacaa tggcatgctg atggtcacat gtagtggaac agctgtctcc acgagataag
      361 taggggacat ctgttttaat aactaaagag agatgggaca tgtagtggca ctcaagaatt
      421 agatttttca gtctaacttc gaggtactct accttcccat cctctctttt ttatttcttt
      481 ttgcggatga ttccgtaagc tgctgcgact aacacacccg cccctgctaa ggaataactg
      541 acagaagctg ttttttcatt tgtttttggc aaagtctgac ttgttgattt ggatggctct
      601 gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa
      661 tgcaaaatat cttttattcg ttggaaacta gctgctaagt cagctttttt tgcttcaaaa
      721 tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg
      781 atatccgctt gagtttgact tagctttcca gaagctgttt gagcaacctg atttaattgc
      841 tgattgaatt cttttgcttg ttggatcttc ccgtcaagat ctgtttggac agcttccgct
      901 tttgctttcg cttcggcaag ctttttctca agttgtgcac ggtcatttcc cactttattt
      961 ttaagatcag ctaaatcttg atcgattgct gcttggtctt ctttcaattt gttttttgca
     1021 gcttctaatg cttcttcgag ttcttttctt ttattttcca agtcttgcaa atgatcgatt
     1081 tggtcagaag aggtagaact atttattgat gtatcttctg aagatgaaat cgtttctcct
     1141 gaattatctg tcggtacatt ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca
     1201 gaagagctta tagttgttga aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct
     1261 gtctgtccca ataagaaaat actggaaaca gcaattggcc agattaacgg cttcattctt
     1321 tttttcatca cttttcccat ccttactact ttttaactaa tttcttatta taagcaaaaa
     1381 tgtttggaag aaccataaac acaacaggct gtttgtctaa aaaacggtgc atcttttatt
     1441 tttttgtacg ttccgggctt ttccatgtcc cgcttccata acctaaaatc gttttgactg
     1501 ctggaatgaa agtcgtcacg atcgttatcg cgttcactat ccagaacaaa agcatatata
     1561 gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc atctacaatc agcgaagcta
     1621 gcaactggaa aaatcctgcg accatttcaa agcagatgaa cacaaaactg aatgtaaaag
     1681 taatataaat ctgttcgtag tttccttgat atacataatg cccgattaat ccagcaaaaa
     1741 gaactacgga aatacaaaag aatattgacc agacaatact caatgtttga tcaatgaaga
     1801 tgatcgttct tccaatatgt ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc
     1861 agacttctgt tccacctttt gcccaacgtt ttctttgtcg atatagcatc ttcaacgtca
     1921 caggtacttc catgaaaaag ataatctcag gagcaaaaac agataaccag tcatttaact
     1981 gatgatccca agcaatactg atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat
     2041 cgatcaaggc ttcttttcga tacatcgtat ttgctccact gtacgcataa agaccaccga
     2101 aaacagctga ctgtgttcgt ttaatgatgc ccacgatact tgagaattca accgtttgag
     2161 attttgcaat caatttggta cgattttgga catccatatt tgcagtgacc gcagcaatat
     2221 gtcttgcacc agggcgaatg aaataattca catacctaat cagtgcatct ggctccggta
     2281 ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa
     2341 acgcgtgggc ttttccttta tttttttcga tccgaaccac acgaagatta gcgtattttt
     2401 tcatcagtct agctaatatt tctggtgttt ggtccgttga accatcgtct gtcactaata
     2461 cctcatagtt gtgataattc aatttcgtca tcaaatactc gattgtatct tcgatcacaa
     2521 tttcttcatt atgggctggc accatgatcg tgatgaacgg ttcgacactc aagggaacat
     2581 ctacccattc tttttgctta tgtttgtata aaaacacata acaccagaca ccaataaacc
     2641 atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa tacataatag acaaacaagt
     2701 atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac cccattttct
     2761 ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac agaataaaaa
     2821 cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc ttcaagtaat
     2881 tcttgtcttt ttttcaattt ttcatcttca tattcaaaag atttatttac ttttttgtat
     2941 agatttcgat tgttccacct agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc
     3001 aatacaaaaa agccgattaa gaaatacagc attttctttt ctgataaaaa agcgtacaag
     3061 gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa agacccatat aaaaggaaga
     3121 acaacgatca tccatcctaa aagagccact aacgtttgcc aaatctttaa ccagtaatta
     3181 ccttttgaaa aatacttatc tttcaccaaa attgatgcaa cttcttctct tgttactttt
     3241 ttcatatcct cacctcacgt ttttttgtta cgacgtagga gtttaccata catttcattt
     3301 cttttcacat aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca tttctcatca
     3361 gcaaatgaaa aatattttgt tctttttatc agttgttctt tttatttttt atctcttaat
     3421 tatgttgctc tttccaatac gacaatgctt ttcattcctc tagatatcgc cgtgtacatt
     3481 tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg gatataagat tacatgatca
     3541 aattccagtc cttttgctag actcaaagga atgatttggc agttttctaa tccatatgaa
     3601 ttttttttaa tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt gataactgcc
     3661 gctttttctc cttttagaga aaccaaaatc tcagctagta ttttcaacaa ttcttcagag
     3721 gatcgaaaag agcggtattc gggttctttt ccttgcggtc ggatcgatac gatgtcgatc
     3781 ttctcttgat ccacagcata ggttttgaaa agctctgtga ttgctccgct agaacggtaa
     3841 ctatttctaa gatcataccg atggatcggc agatggtagt cgtcgaaaca acgcataata
     3901 tcagcaaaag tagtggaaga attaaagatt gcctgattct cgtcacctac taaagtaaag
     3961 tgagtttttg gaaataattc gatcaataga cctaattgtg ccagggtata atcttgtact
     4021 tcatcaatca atagatagcg aaatgctggt acatttacct tttcaacaag taaatgacga
     4081 atagctaata taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag
     4141 ccatatcgtt tttcataaat tctttcaaac aactgatgct catccaccca tgccatttct
     4201 tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa gtttccttgc ataagctaca
     4261 atactttgtt ccgaatcatc agaaatcaat ttaccgaata attccaactg cctatcttct
     4321 gtcaacgaga gtacctgatt ctgcacagca ggactttttg cttgtttcaa aagatgattc
     4381 ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga
     4441 gcaggaactt tctcatatat tttttcgatc gtttttttag aaaggactgt ttttccattt
     4501 ttagtaagtc cacggataaa attcggatga tctaaaaaca aaggatctga ttgttttaga
     4561 ttatcgataa actttttgct acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg
     4621 atacgtttga aataagcctc ttcaccttct atttcctctg ctgaaagctg tgaaaccagc
     4681 tgtatgatgg ttagattcag tggatttctt tcacctaatg atggtaggac attcgaaata
     4741 tagtcgataa accgatgatt aggtgataga atcagcaagt catcggatgt catcgtatct
     4801 cgatataaat agagcagata cgcaatacgc tgcatgatag cagaggtttt accacttcct
     4861 gctacgccat ttaccaagat attttgactt ttcacatccc gaatgattgt gttttgttcc
     4921 gattggatcg tagcagtgat agccttcatt tcattggtct catcttggcc taagacttct
     4981 aataatacgt catcttgaat agctacagac gtatcaaaaa acttgaccaa acgatctctt
     5041 tccaaaagaa attgtcgtct ttctttaata accgtatcga ttttattttg acgaaccgta
     5101 taagaagatg gaccaatcat gttattgtaa aaaagttcgg caattggtga acgccaatca
     5161 taaacaagat tttcatctgc atcatctgtg aaatttgctg taccgatata aaaggattct
     5221 tttggctcat tatccagaaa gtctacaacg acttttccaa agtagggact tttcaataaa
     5281 cgttcgatct ttgctagctg attttctgcg tttttctgtt tgatattcat ctgatcgatt
     5341 tctctatttt tcatttcaat catcgaaaaa gtatccaggt tatctaagta actgtcaaaa
     5401 ttcaatcgaa gctcgccagc catttctttc aacgactggt ttccttctat ttttgccgta
     5461 tccatcgatt cccgcagttc gtttttttta tcaattagct tttgatacac gttcttcaga
     5521 tatgcttctt ctaactgttt ttgtgtgttg accatacaca ttctcctctt tttttaaagt
     5581 ggtggaaccg attagtttac ctcttttaag agtcgaatgc aagcttttta acgagtatca
     5641 tttgttagga tatatcagtt cttcaggtcg gacagtttga tataaagaaa atgcaccatc
     5701 aaggtttttt acgtggtagc cagactgctt caagattctt tccgctagat agctacgtaa
     5761 tccgctatga cagctgacga tatattcttg ttggctgtct aattcattta atcggtcccg
     5821 aagttcattc aaagggatat gtttggcttc cttgaatttg cccttttcca attcttctgc
     5881 tgttcgcaca tctaataaaa tttttccttt tgctagttct gtcggcaatt catgccattg
     5941 tacggtttca ctaagccctt ctgcaatgtt cattgctgca tatcctagca tgtttactgg
     6001 atctttggct gagccaaatg gcggtgcata cgtcaattct aattccggta aatcgaagat
     6061 cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc cgtttatcca caccttttgc
     6121 cccaacacct tgtgcaccat aaatctcgcc agtctctgga tggaagatca acttcagtaa
     6181 aatatctgtt gcttctggat aatagcttgc atgatctttt cctgatacat gaatgacttc
     6241 aaaagacagt tcgttttctt tggctgttct ttcacttaaa ccggtggatg cggcagtcat
     6301 tccaaacgtt cgtataattg ctgttccaat accgccttga tttcttcgag aaattccgga
     6361 aatcgtgtcc gcaacttgtc taccttgacg attggctggt gaagcaagag aaataagtgc
     6421 atcttttcca gtgatttgct gttttacaac gatagcatcc cccaccgcat aaatatcagg
     6481 aatattcgtt tgataacgtt cgtctactag gatacccccg cgtatgccta attcgattcc
     6541 agcggtcact gctagctggt tatcaggacg aacaccaatg gataaaataa ctaaatcagc
     6601 agggatggta cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac
     6661 agcatgagaa gtaatcactt ctactccttg atgagacaat tctttttcta taaaagctgc
     6721 catttcttca tccagtgttg gaagaatatg tggtgcttgt tcgatcacca tgacttctaa
     6781 acctcttctt tttaggtttt ctgccatttc caatccgatg aatcctgcac caacgatcac
     6841 ggcacgcttt ggctgttttt ctaatgcatg catcacttta tcgatatctg gtacatttct
     6901 aatagaaaaa acattgtcgg cttctgttat ccctggaatc gatggaacaa ctggttttgc
     6961 accaggagat aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct catgtacgac
     7021 ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca tgatttggac gcacatctaa
     7081 acaaaaacga gcggataatg attctggcgt ttggacaaga agattttccc gttcactgat
     7141 ttctcccgaa agataataag gcaagccgca atttgcaaaa gaaacatacg gtcctttttc
     7201 aaaaacaacg atttctgcat cttccattaa tcggcgaagc cgtgtagcag cagacattcc
     7261 acctgctaca cctccgacaa taacgatttt catcttattt ccctcctact gttcgatcaa
     7321 accattgatt catgccacca cgtacattca ctacatcata accagatttc tttaattctt
     7381 tagctgcttg tttactcctc atccctgatt gacaaatcac atagacggtc ttttctttgt
     7441 ctccatcata tctgtttata ctttgtaatg gaacattttt agctcctttt atatgcccgc
     7501 cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa agcttttcct
     7561 ttaattctgg gactgttatc gattttattg attgaaatag caaattcatt ttctgcacct
     7621 catttacatt tttataaata cctcatgggg tatattaaac aggagcttta ttaatatgtc
     7681 aaccagaatg actcttcatt tttttcacta ctttttatat ttgcataaaa taccgataag
     7741 gggtattatc taattataaa aatagaggag aatgtgttat ggagattgat aataaaaata
     7801 tcctgaaccg tttgaaacgt acagagggac aaattcgtgg tatccaaaaa atgatcgaag
     7861 acgaaaaaga atgtatggat gtcatcactc aattaagcgc tgttcgttct agtattgatc
     7921 gcgtaatggg catgatcgtt gcagataatc tgaaaaattg ctttgagaaa cctgatagta
     7981 atccagaaga acaagcggct aaactagaac aagcgatcaa gatgattatt aaaaagtaaa
     8041 ggaatgattt caatgaaagt cgttttccat attgatgaat tagaaaaatg gtcagaaaca
     8101 gggaaaaacg taaaaaacct gataaaagct tctccagaaa cgacgattgt cgtatctgta
     8161 aatggcatcg cgattaccgg ttatctagac tctgccaatg cagaattctt agatttgcaa
     8221 ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta atcatatttc agaaagttct
     8281 ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg atttagttga actgcaatcc
     8341 caaggttatg cttatatcaa gccttagtca gtagaggttg cgacagaagt gatcagcttc
     8401 aagaaatttc ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg
     8461 tgaaaacgct gtcctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
     8521 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
     8581 gttgtgaaat cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca
     8641 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag
     8701 agattgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggatttc
     8761 tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg
     8821 aagaggttgt aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct
     8881 gctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac
     8941 caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact
     9001 tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga gtctatttat
     9061 caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg
     9121 gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat
     9181 gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata
     9241 gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga
     9301 tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa
     9361 cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt
     9421 atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat
     9481 gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa
     9541 tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa
     9601 aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc
     9661 ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat
     9721 ttcactgttt ttatttttct ttacagccac tgatattatg agctaatttg agataatcac
     9781 ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt
     9841 actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa
     9901 tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc
     9961 agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta
    10021 tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac aaatatccga
    10081 tcaccttttg caaagagctg gcgttctttt ttattctcct tcctatttgt aactgctgtc
    10141 ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct
    10201 tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct
    10261 cctgtttctc caacccgcaa ctgatattta ggacgaagtg cttcccgatc gaaagccata
    10321 gcagccggca caaaatcttc atgatctccg gcgaatttct tgatttctcc ataatgggtc
    10381 gtggcaatga tcaatgcccc ttgctcatac atagattcca tgattgcgat cgctagtgca
    10441 gccccttcat ttggctctgt tccactgcca atctcatcta aaagtaccaa cgtattcctt
    10501 tttatctttt tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt actcaacgca
    10561 ttttctaaat tttggtggtc accgacatcg acgaaaatct catcaaagac agcaagctct
    10621 gtcccttctt ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa taaaccgact
    10681 gtcttcaaca cgactgtttt gcctccggca tttgctccgg tgataatcaa gcctcgatag
    10741 tctttaccga tttcaacatc caatggtacc gcatgctcag gtaaaaatgg atgtctgcct
    10801 tgcttgataa tgatatgttc cgatttattg attctaggtg tgcttccatt gatttctcgg
    10861 ctaaattttg cccgagcaaa gatgatatct aacaccgtga tcgtttcgat caataaatcg
    10921 atcacttctt cgttttctgc gatagcacca gtcaatgccg ccaggacttg atattcttca
    10981 ctgatttctt cagcttttaa caactgatag tgttcattca atttagaaat cacagttggt
    11041 tcaataaaaa ccgtcgttct tttatttgat tcatcgataa tcgttccatc tattttattt
    11101 ttatagctgg ctttgatagg aatcgtatag tattcgcctt tttgaacaat cattgcttct
    11161 tggatcattt ctttgttttt cggatgacgc aagaatttca gtaattttga ctggatttct
    11221 ttttcgatta gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg
    11281 actttattat tttttatttt ctggtcaatc aactcttcta catttatcaa atcaggaaga
    11341 tgtttgctat aagcaaacaa caagggtgtc tggtattgat tcttatcaaa aaactttgtg
    11401 atcatccgac tgcttctaag aaaatccgca tattcgatca aatcggttgg ctgcagaatc
    11461 aacccttttt tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca
    11521 tgctgactgc tttctaagat caagcgtgct tctcttgttt cttcttgacg atccaagaca
    11581 gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata gtttccaatt
    11641 gctcttgctt ggatttcctt tttgatccga tcaaattgta attgactaat catttcgttg
    11701 tccatattct ctccttttct tgaagagata cctgtcccaa aataaataag ggcctagaga
    11761 taaataaaag gatagaacac agcttaaaaa agtcatgtcc tatccttatt agaatacaaa
    11821 gatattgttt tcctgacagg taccgtttca taagcacacc aaataaactt tgtttattgg
    11881 ctattcgact tactgattag ataccagcta cactcacaaa acatacccca ttcttctagg
    11941 tcttatttat tttccactta actatagcaa gacacatttt ggttgtcaaa ctttagtatg
    12001 ttttatagat ttacaccatt aatgaataga cacctaccgc gataattgct ccgatcattg
    12061 gacctgcgat tggtacccat gaatatgccc aatctgaagt tcctttatta gctattggca
    12121 ataccgcatg tgctaatcga ggaccaaagt cccgagcagg gttgattgca tatcctgttg
    12181 gccctcctaa tgaaagaccg atggctaaga tcaatccacc tacagcgaat acgttcgttc
    12241 caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag aactaataca aatgtaccaa
    12301 ttaattctgt gatcacattt gctggataat tccgtatagc aggacctgtt gcaaacgttc
    12361 ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg caaataagaa agccaaacaa
    12421 gaatggcccc tgcaaaagct cctaaaactt gcgcaatgat gaaaggaacg acaagattcc
    12481 aactaattgc ccctgtcatc gccatagcta gagagactgc aggattcaaa tgagctgggc
    12541 ccatgaatcc agaaatatag acagccattg ttacagcgag tccccaacca aatgcgatga
    12601 caatccagcc agaagcctgt gctttacttt tgtttaaatt cacagccgca cacacaccat
    12661 ctccaagcaa aactaggatc agtgtcccaa taaactctcc catgatttga gtcatatcac
    12721 tgttcattta tttctccccc tttagttccc gcagatctga ttctgcaatc aatgcttcta
    12781 aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca gcaattgcat
    12841 cgatcacagg ttgcttgatc tcatcaagct gatctcgttc aaacaacaga tgatttgtac
    12901 gacgaatgag ataatcgcct ggggccaaaa ccatttcttc ttctaaacca taacgtaacc
    12961 gagctgactc agcgagactc aaaccaggat aaggtgccat ttcttttgct aattcaaaga
    13021 tacgtcgggc attagtcccg taaaaatcag cgatataagt agcatcttct tcctctaaac
    13081 cggctgctac tccttctttt gctaattctg tcactgtttc ttctaattta gttggatcga
    13141 agtttccacc tgaaatctga tattttttag agtcgatttc tttcgtctct attccgtatt
    13201 cttcttttaa taattggcgt atcaatcgca aagctccttc tgccatctta cggtaatcgg
    13261 tgattttccc accagataaa gtcagcaaac catctggttc tctttctaaa gaactacctc
    13321 tggaaatcgt agaaggtgct ttttcatcac ggctgttttc caaatggttc aacacgtctt
    13381 ctacttcagc acgagaaact ttgttttcct tatattcact tactgtatcg acaactttat
    13441 tgaagctctt gtctgaaatc gatccattat ctccaccatt gtaatcagaa ccagaattac
    13501 caatcaacag tggacgaagt cctgcccagc ttgcttcgat atcattcaat gtgatatttg
    13561 cttctggata gcgatgattg atcacatcca atagataatc tacatcttct tgtgtgactt
    13621 tcggatccgt aaaatctcct tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt
    13681 ctcttggaat agcaaaaacc atccgcccat cttgctttcc tgtatcgaag tatgtgggtt
    13741 gcggtacagg cagttttttc gcatctacaa ctaaatggat ccctttagtt ggacgcattt
    13801 taggagagac tggacgcgta aaattcaagt ttcgtacttt atctacccaa ggaccactcg
    13861 tattgatcac taattttgct ttgatttcga tcacttcatc tgtcagcagg tcacgagctt
    13921 tgacgccaac gatttgatcg ccatcatata aaaagccaac cgctttcatt ttacttacta
    13981 gataagcccc atcttcggca gcctttttga tattatcaat cactaaacgg gcatcattgt
    14041 tgcggaaatc tagatagaca cccgcgcctt ttagcccttc ttttttcaaa aacggttctc
    14101 tttccaatac ttcttctggt gtcagcgtat aattctcata tttggttcct gtcacatttg
    14161 ccagtttgtc ataaaggtcc attgctactt tgaccgagaa catattgaag gttgttgctc
    14221 cttcgccctc atagattggc aaaagcattg gatctggttt tgggatgtgt ggggcaatac
    14281 tttgtacgac tgcacgttca ccaactgtgt cagctactac ttccacatca aatgtcttca
    14341 gataacgaat accgccatga acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat
    14401 cctgcatttc gatcaatcct gttttgattc ctgatgctgc tgactggact gccacaccgg
    14461 caccagtgat tccgccaccg atgatcaaca gatccaattc ttcttgctgc attttttgaa
    14521 tgctatcttg tcttgtcttg tttgaaaaca tatggatttc ctccttattc aaatgcttgg
    14581 gtagctttta ctgctttttt ccaaccgttg taaagttttt ctcggcgctc ttcctccatt
    14641 tgaacttcaa atagcttgcc agcttcataa aattcgcgga tctcgtctgt atctttccaa
    14701 taaccgacag ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc caagttttct
    14761 gctcgttgga tcggtacatt caagatatct gtttggaatt gcatcagata ttcattgttt
    14821 gctgcaccgc catctacttt caatacagga atcttgattc cagtgtcatc ttgcatcgta
    14881 tcgatgatat ctctcacttg ataagcaatc gactgtaaag tagctttaat gatatcttct
    14941 tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc ccaataagga
    15001 gcacctagcc caacgaatgc aggaacaaca taaacctcat cttcgcttgt agagcgtttc
    15061 gctgcgtctt ctgaatcgct agctttttgc aacatttgaa gtccatctct cagccattga
    15121 atcgaagaac cagccacaaa aatacttcct tctaaagcat agtaaacctt tccatttatg
    15181 ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt gcggtttttc accggtattc
    15241 atgacaataa atgaacctgt accataagta ttcttgacca tgcctggttc gaatgccatt
    15301 tgtccgaata atgcggcttg ctggtctcct gccatccctg aaattggtgc ttcgcttcca
    15361 taaaaatgat aaccttgcgt ataaccataa acttctgagt tagaagtgac ttttggtaac
    15421 atcactcgag gaatatttag taaatctaaa atctcctgat cccaatctag ctgatggata
    15481 ttgaacaaca tcgtccgact ggcattcgag taatcagtaa catgtgcttg tccatcggtc
    15541 agtttccata ctaaccaaga atcgatcgtt ccaaacatca attcgccttt ttcagctcgt
    15601 tcttgcgcgc cttctacatg atcaagaatc cagcgtatct tcgttgctga gaaataggca
    15661 tcgatcacta accctgtttt ttcgtggaaa aattcttgat agccttcatt ttttaagtta
    15721 tctgcaattc cagctgactg tctggattgc caaacaactg catgatagat tggacgtcct
    15781 gtatcttttt cccatattac tgttgtttca cgctggttcg ttatgccaat cccagcaatc
    15841 tgatttggtt tgatccctga ttcgatgaac gctccggcaa taactgactg tacagaattc
    15901 cagatttcat ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg cgtgaactct
    15961 ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg tgagctagtt
    16021 gtaccttggt caatcgccat gatatacgtt gattctgtca ttgtgattcc ctccattata
    16081 tgcaaacgct ttctttaact actcttatca tactactgtt ttaaaaaaag aaactgtcaa
    16141 aattttattc tttttttctt tttttgacag ttttttatta tgaagctatt ttttaatcaa
    16201 ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc gtaaggtgac
    16261 gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc
    16321 aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat tcccctcagt
    16381 tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc taaatcgatt
    16441 ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt ggcatactgg
    16501 atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg ctgcaccgct
    16561 gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt
    16621 tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa ccattcattg
    16681 ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa acgacgagga
    16741 cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt tggtaacttt
    16801 ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga aaaagaaaca
    16861 aaaaagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt tcgactaaga
    16921 tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata gagtaatttc
    16981 ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt cgtaatcccg
    17041 agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc
    17101 aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg tcgctgatct
    17161 tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc ttctccgtat
    17221 aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa ttctttgatc
    17281 tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac gatggatact
    17341 tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg caataaaaac
    17401 aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg atactcattt
    17461 cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc ttccaaatat
    17521 tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat agaggctgtt
    17581 cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt tctttctaat
    17641 aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat ctggcacacg
    17701 gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc atccttcttc
    17761 tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt ggagctgtgc
    17821 tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc tttgatttat
    17881 tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa aaatgctctc
    17941 ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc cgactggacg
    18001 ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt
    18061 acggacagcg ttatttacta gagaaacata cattttttca tttgttggac gaaaagttac
    18121 ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt agtcatctac
    18181 ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg
    18241 agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg tctctacaat
    18301 agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg aaagcccttg
    18361 atttttcatt tcctttacta acggttctgt catttcagga tctagataac gaaaaagaat
    18421 tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc cttcagctat
    18481 ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca tgatcttttg
    18541 ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg attctgcaga
    18601 aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa aatccaattt
    18661 ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct tttctgtgta
    18721 aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa gttgtttttc
    18781 actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc ctaacttcga
    18841 ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat aaatagccgc
    18901 atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc ctctttctta
    18961 taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac tcgctttcaa
    19021 ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt gttctaggtc
    19081 tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga agagatgttt
    19141 caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag
    19201 gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt tagaatagac
    19261 acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat tgatgagcag
    19321 attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct gaaaaagctg
    19381 ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag aatggtactt
    19441 cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact gtacgtccag
    19501 ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa
    19561 cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca agatctattg
    19621 cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct gcataatcat
    19681 ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat
    19741 cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt tctaaaggta
    19801 catatccgcc tttaggactg acaaaatctg catcgatccc attttttact aatacatcat
    19861 aaaaatgagt cagttcactt aaccacaaac ctgtaggaag ctcgatcttt tcgtactttt
    19921 ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat aaaaattgcg
    19981 ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa gctctttata
    20041 tgcttcttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata atttctctgc
    20101 ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat atttcaatac
    20161 ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga tctttccttg
    20221 aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag aacgagctat
    20281 tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac gtaatcgttc
    20341 aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt ctggtgagaa
    20401 acgataccct acgatgaatg gttttttagc atattgttca accgctgaga aaaccgttgt
    20461 taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt gatcctctcg
    20521 tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc catgcaactc
    20581 gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg cttggattgt
    20641 ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac gttttgctgc
    20701 aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg tcattcgtcc
    20761 accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt tcgctagctc
    20821 acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc agccttttcc
    20881 gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag aacgttgttt
    20941 ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat
    21001 aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc
    21061 aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg agaatcatct
    21121 atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa ttttattttt
    21181 tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt gcttcatttc
    21241 ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact tccttagcaa
    21301 attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta actgttttga
    21361 cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg gcggaataga
    21421 gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc aataaaataa
    21481 aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat ttattcaaca
    21541 tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt tcaattggct
    21601 tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat tttttgtcaa
    21661 aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag tagccatctt
    21721 caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa tcagaggttg
    21781 tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc tttccacatt
    21841 tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt atatcagagg
    21901 ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    21961 attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt ttattgagag
    22021 gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    22081 tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact gtttattcgt
    22141 aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta ttataggatt
    22201 tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt taaccgtttc
    22261 tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg ttttgacatt
    22321 tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc aaataaatct
    22381 actccatctt tgatagcttc cttcatctgc tcaatatgct gtttcaaata gtcaatgcgg
    22441 taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag tccgttttca
    22501 gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat tctcaaaccc
    22561 ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt aattcctcca
    22621 gttattaagt ttccttggac cccttcccaa ttttctggat gagcactgac tgcactagac
    22681 atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct
    22741 acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa
    22801 tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt
    22861 tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg
    22921 acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac
    22981 tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact
    23041 cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat
    23101 tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata
    23161 agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg
    23221 gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa
    23281 aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat
    23341 gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta
    23401 aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc
    23461 tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta
    23521 ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc
    23581 catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat
    23641 tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac
    23701 tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat
    23761 atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt
    23821 tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac
    23881 cgatatggat cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg
    23941 gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga
    24001 tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat
    24061 caattatttc acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga
    24121 tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat
    24181 aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc
    24241 ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc
    24301 cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag
    24361 gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt tttactttct
    24421 tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca
    24481 tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta
    24541 caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac
    24601 cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg
    24661 cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa
    24721 tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa
    24781 tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta taccttgcta
    24841 gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg
    24901 taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt
    24961 ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat
    25021 tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga
    25081 gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg
    25141 cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag
    25201 atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat
    25261 ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt
    25321 gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg
    25381 cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg
    25441 ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc
    25501 ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttataa
    25561 tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg
    25621 cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct
    25681 tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt
    25741 ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta
    25801 ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt
    25861 gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg
    25921 tcagcttttc acgcaaaatc tgataaatct cccaagattc aaactcatct agaacactag
    25981 gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat
    26041 catattccgt tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt
    26101 ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa
    26161 ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg
    26221 caagatacaa aacaaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg
    26281 ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa
    26341 aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta
    26401 gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg
    26461 tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca
    26521 aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat
    26581 agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat
    26641 acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag
    26701 aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta
    26761 atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc
    26821 ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt
    26881 cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc
    26941 gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga
    27001 aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa
    27061 tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga
    27121 tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact
    27181 ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt
    27241 tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc
    27301 ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct
    27361 ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa
    27421 aatcatttga agaaagatct atattcaagg tttttaccat tagactggat aatttgttta
    27481 taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta
    27541 tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg
    27601 attggtcccc accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt
    27661 tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca
    27721 tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg
    27781 tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt
    27841 tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg
    27901 cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct
    27961 gccgtgttag cttgcaagca ctgttcgtct ccatctaaca ttactggtgc acagtcttct
    28021 tccttccatg tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa
    28081 aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat
    28141 gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt
    28201 ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa
    28261 atcacttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc
    28321 aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa
    28381 tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg
    28441 attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa
    28501 tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca
    28561 taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc
    28621 catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct
    28681 tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa
    28741 gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc
    28801 ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat
    28861 tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata
    28921 actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca
    28981 agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt
    29041 aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc
    29101 taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt
    29161 gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg
    29221 aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc
    29281 atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc
    29341 ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac
    29401 agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg
    29461 aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt
    29521 cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc
    29581 agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc
    29641 atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt
    29701 catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt
    29761 tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc
    29821 ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa
    29881 gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg
    29941 gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt
    30001 tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat
    30061 ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt
    30121 gccaatcata tcgatcacgt ccatacgaaa gcccccaata cctttatcga tccagaaatt
    30181 catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg
    30241 cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc
    30301 actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata
    30361 atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc
    30421 agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc
    30481 aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact
    30541 gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc
    30601 aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc
    30661 atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca
    30721 ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat
    30781 ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta
    30841 tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag
    30901 gattgattta tgcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc
    30961 agctgtccct tggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttcgtttt
    31021 tttctctaat actcaggcca gaacactgat ttcacaacct ctttataaac ggtgttcact
    31081 cagctgtccc tcggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt
    31141 ttttctctaa tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata
    31201 tccacatgaa tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc
    31261 ctcttttagc tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg
    31321 aaatactata agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta
    31381 agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg
    31441 ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca
    31501 agaagaaaat ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag
    31561 ctgatgattc tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag
    31621 ctgttgaatc agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac
    31681 ctgcttggtt tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga
    31741 atgtcagcac tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa
    31801 ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca
    31861 aacctgtgac cagacctaaa atcgttgtag ctgctatccc gatgaagatg ccacctggta
    31921 cacgccgaac aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac
    31981 caacgttgag tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt
    32041 cgctcagacc aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat
    32101 ctgctgggat cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa
    32161 tcaaagaagc aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac
    32221 aaacagaata agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac
    32281 gggctaatac ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca
    32341 ctgctccttc atccattcca gaagcaccta atacagttgg atttacaaat aagatataag
    32401 ccattgagat aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt
    32461 tttctatttc gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac
    32521 attataaata aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat
    32581 actaaaacaa aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca
    32641 tctgattaaa tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc
    32701 tttattgtgt taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt
    32761 ttaccatttc gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta
    32821 tttagtccgc caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct
    32881 gatttgagat tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa
    32941 ccttctaatc ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc
    33001 agcttttcct ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc
    33061 attgttatct gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc
    33121 cgtaaaaaat actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa
    33181 tacttttggg aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa
    33241 ttcttctggc aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg
    33301 gcaaatcatc tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg
    33361 acccttcttt ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa
    33421 gcgcaaacac cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg
    33481 ctttcctcca caaacttttt ttacaaaaaa aaaaaacggc aatgaatgcc gctcttctta
    33541 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    33601 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    33661 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    33721 agcattgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    33781 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    33841 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    33901 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    33961 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    34021 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    34081 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    34141 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    34201 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    34261 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    34321 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    34381 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    34441 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    34501 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    34561 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    34621 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    34681 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    34741 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    34801 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    34861 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    34921 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    34981 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    35041 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    35101 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    35161 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    35221 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    35281 attttgtttg taagactagt ttacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    35341 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    35401 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    35461 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    35521 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    35581 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    35641 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    35701 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    35761 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    35821 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    35881 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    35941 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    36001 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    36061 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    36121 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    36181 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    36241 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    36301 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    36361 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    36421 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    36481 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    36541 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    36601 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    36661 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttga
    36721 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    36781 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    36841 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    36901 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    36961 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    37021 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    37081 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    37141 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    37201 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    37261 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    37321 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    37381 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    37441 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    37501 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    37561 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    37621 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    37681 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    37741 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    37801 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    37861 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    37921 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    37981 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    38041 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    38101 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    38161 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    38221 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    38281 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    38341 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    38401 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    38461 catcggttgc tgtagcattg atgaatccca tcgaattaaa gatcgttact agcaaagcgg
    38521 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    38581 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    38641 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    38701 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    38761 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    38821 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    38881 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    38941 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    39001 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    39061 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    39121 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    39181 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    39241 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    39301 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    39361 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    39421 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    39481 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    39541 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    39601 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    39661 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
    39721 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    39781 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    39841 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    39901 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    39961 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
    40021 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    40081 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    40141 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
    40201 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    40261 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    40321 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    40381 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    40441 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    40501 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    40561 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    40621 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    40681 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    40741 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    40801 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    40861 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    40921 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    40981 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    41041 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    41101 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    41161 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    41221 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    41281 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    41341 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    41401 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    41461 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    41521 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
    41581 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    41641 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    41701 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    41761 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    41821 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    41881 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    41941 caacaaaaaa actttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    42001 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
    42061 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    42121 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    42181 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    42241 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    42301 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    42361 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
    42421 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
    42481 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    42541 ctatctctgg cacactgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    42601 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
    42661 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    42721 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    42781 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    42841 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    42901 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    42961 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
    43021 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
    43081 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
    43141 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
    43201 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
    43261 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    43321 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    43381 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
    43441 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    43501 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
    43561 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    43621 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    43681 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    43741 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    43801 ctagctcctt agctgcttgg attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    43861 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    43921 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    43981 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    44041 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    44101 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
    44161 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
    44221 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
    44281 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
    44341 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
    44401 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
    44461 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
    44521 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
    44581 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
    44641 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
    44701 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
    44761 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
    44821 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
    44881 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
    44941 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
    45001 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
    45061 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
    45121 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
    45181 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
    45241 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
    45301 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
    45361 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    45421 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    45481 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    45541 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    45601 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    45661 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    45721 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    45781 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    45841 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    45901 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    45961 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    46021 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    46081 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    46141 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    46201 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    46261 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    46321 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    46381 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    46441 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    46501 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    46561 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    46621 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    46681 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    46741 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    46801 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    46861 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    46921 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    46981 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    47041 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    47101 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    47161 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    47221 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    47281 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    47341 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    47401 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    47461 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    47521 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    47581 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    47641 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    47701 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    47761 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    47821 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    47881 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    47941 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    48001 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    48061 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    48121 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    48181 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    48241 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    48301 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    48361 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    48421 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    48481 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    48541 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    48601 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    48661 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    48721 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    48781 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    48841 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    48901 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    48961 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    49021 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    49081 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    49141 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    49201 agggatatct acacgtggta aagaatcgac aacttcgttg acttgaacga tcacgatacc
    49261 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    49321 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    49381 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    49441 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    49501 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    49561 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    49621 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    49681 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    49741 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    49801 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    49861 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    49921 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    49981 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    50041 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    50101 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    50161 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    50221 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    50281 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    50341 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    50401 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    50461 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    50521 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    50581 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    50641 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    50701 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    50761 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    50821 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    50881 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    50941 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    51001 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    51061 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    51121 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    51181 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    51241 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    51301 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    51361 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    51421 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    51481 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    51541 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    51601 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    51661 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    51721 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    51781 cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    51841 aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt tgatttaaaa
    51901 cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag atcaatagag
    51961 atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt
    52021 ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg gtcacttgca
    52081 tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt tttgcttttt
    52141 tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg agacaaattg
    52201 tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt ccatctcttt
    52261 cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa tacaagtagt
    52321 tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt caatccgcct
    52381 gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc acctattttt
    52441 tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca gtaaatgctc
    52501 cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt attttctccg
    52561 tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa atcattaccg
    52621 aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat acaaagataa
    52681 ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt tctgaccttc
    52741 aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa gcaggaatca
    52801 gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt ttcaaacaat
    52861 cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct gtccctgatt
    52921 ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact aaaatcaatc
    52981 agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct tgtattcttg
    53041 gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc actcataaca
    53101 actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt ttaagaaacc
    53161 tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt tggttcttta
    53221 tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat accaacagca
    53281 cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa ggtatttatc
    53341 ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg aattacaaca
    53401 ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta gaggtcctcg
    53461 aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa aaaacatctc
    53521 tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata ttgattgaag
    53581 cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat gcacacttgt
    53641 atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc aatgaaccaa
    53701 attactttct ccaactcttg tctctactga tccaacaaac gattttttct atcaaaccga
    53761 atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg ttcttgcagc
    53821 aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa ctaaccgatc
    53881 tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct ctagattctc
    53941 tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc aatcgtttga
    54001 ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc tctagctgtt
    54061 ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac aactaagaat
    54121 agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact tactgtaccg
    54181 atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc aaggatacct
    54241 aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg cttggacaac
    54301 tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag aagaatggtt
    54361 tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac catgatcagt
    54421 agggagacat agatgatccg aaacaaatga taaccagata ggattccttg ctttggatct
    54481 cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc ataagataaa
    54541 gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg tagcgggcga
    54601 ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg caagaagaaa
    54661 ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc ttttgcaatc
    54721 gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct
    54781 ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc atttacgccg
    54841 tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat tcgcagttta
    54901 tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat caactcttca
    54961 tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt taagccaatt
    55021 tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt cactgagatc
    55081 cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg atcgatcaat
    55141 ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc ctgtttcgaa
    55201 tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa ttctgcagct
    55261 gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa taatacttct
    55321 ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt tgccatatat
    55381 ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag attggctgtt
    55441 tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc tgttttctgt
    55501 tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca ggcttcttct
    55561 tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt cttatctgaa
    55621 cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat gatcgccttt
    55681 tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc tggcaatcct
    55741 tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaaggatatg ccaatccata
    55801 ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat gccttgaaaa
    55861 atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg ttcgaccgat
    55921 tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg
    55981 actgcagaac cagattgtac aagcgttcct gaaaaagcca tatttttctg gtcgccgatc
    56041 gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc tcccgtcaaa
    56101 atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc tggaacgaca
    56161 tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg gattttttct
    56221 tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaat
    56281 gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat cactacgaat
    56341 aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga ggcaatgatc
    56401 aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg ttttgtctgc
    56461 tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg ttcttcttgc
    56521 gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac
    56581 cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt
    56641 caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa tgtatctggg
    56701 cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga
    56761 taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg
    56821 aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt
    56881 ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg
    56941 ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac
    57001 aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    57061 tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga tataaaatcc
    57121 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    57181 tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact aaaggacttt
    57241 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    57301 agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc ttttgcggat
    57361 cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg aataaaacca
    57421 taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg atctgtccaa
    57481 aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat cctataccca
    57541 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    57601 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    57661 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    57721 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    57781 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    57841 ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    57901 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    57961 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    58021 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    58081 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    58141 gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaat
    58201 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    58261 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    58321 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    58381 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    58441 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    58501 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    58561 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    58621 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    58681 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    58741 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggattttcc
    58801 aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    58861 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    58921 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    58981 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    59041 atatcaaatt tcaaattcaa aagagtgatg atccaataaa cgactaaaat gacaactaat
    59101 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagcg
    59161 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    59221 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    59281 ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    59341 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    59401 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    59461 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    59521 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    59581 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    59641 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    59701 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    59761 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    59821 ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    59881 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    59941 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    60001 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    60061 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    60121 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    60181 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    60241 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    60301 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    60361 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    60421 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    60481 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    60541 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    60601 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    60661 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    60721 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    60781 tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    60841 ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat ctaacgcctt
    60901 catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt ggatattttt
    60961 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    61021 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    61081 tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac gggcgtacca
    61141 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    61201 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    61261 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    61321 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    61381 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    61441 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    61501 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    61561 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    61621 tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat ctgtaacacc
    61681 tcttcactat agcgccaaaa atgagaaatt tttttttata acatcaaatc cacgtagatt
    61741 tttacaaaca aaaataaata aagtcggatt tttctgataa ttcaacgcaa aaaagcgaac
    61801 aatttttcaa taaatcagtc ttctaaaata gataacctaa aaaaagcgaa caaacaaata
    61861 aaatgactgt ccttcattca ctaaacatat ttttattttt ttatattgtt aaaaattcac
    61921 aatttatttt ttaatagaat tatctgtttt ccaaatttta taaca
//
LOCUS       DASJOX010000004        60192 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJOX010000004 DASJOX010000000
VERSION     DASJOX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.004"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="U4A12_000220"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="U4A12_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN1772635.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="U4A12_000221"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="U4A12_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN1772636.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="U4A12_000222"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="U4A12_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN1772637.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="U4A12_000223"
     CDS             1642..2274
                     /locus_tag="U4A12_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1772638.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="U4A12_000224"
     CDS             complement(2276..3181)
                     /locus_tag="U4A12_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1772639.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="U4A12_000225"
     CDS             complement(3266..3925)
                     /locus_tag="U4A12_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1772640.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="U4A12_000226"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="U4A12_000226"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN1772641.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="U4A12_000227"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="U4A12_000227"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN1772642.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="U4A12_000228"
     CDS             6168..7253
                     /locus_tag="U4A12_000228"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN1772643.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="U4A12_000229"
     CDS             7250..8314
                     /locus_tag="U4A12_000229"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN1772644.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="U4A12_000230"
     CDS             complement(8563..9366)
                     /locus_tag="U4A12_000230"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN1772645.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="U4A12_000231"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="U4A12_000231"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN1772646.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="U4A12_000232"
     CDS             complement(10244..11251)
                     /locus_tag="U4A12_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1772647.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="U4A12_000233"
     CDS             complement(11258..11902)
                     /locus_tag="U4A12_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1772648.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="U4A12_000234"
     CDS             complement(12002..12760)
                     /locus_tag="U4A12_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN1772649.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="U4A12_000235"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="U4A12_000235"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN1772650.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="U4A12_000236"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="U4A12_000236"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN1772651.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="U4A12_000237"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="U4A12_000237"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN1772652.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="U4A12_000238"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="U4A12_000238"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN1772653.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="U4A12_000239"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="U4A12_000239"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN1772654.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="U4A12_000240"
     CDS             18158..19027
                     /locus_tag="U4A12_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN1772655.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="U4A12_000241"
     CDS             complement(19153..20052)
                     /locus_tag="U4A12_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772656.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="U4A12_000242"
     CDS             complement(20217..21041)
                     /locus_tag="U4A12_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1772657.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="U4A12_000243"
     CDS             complement(21052..22347)
                     /locus_tag="U4A12_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN1772658.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="U4A12_000244"
     CDS             complement(22363..22842)
                     /locus_tag="U4A12_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN1772659.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="U4A12_000245"
     CDS             complement(22848..23876)
                     /locus_tag="U4A12_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN1772660.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="U4A12_000246"
     CDS             complement(23893..24999)
                     /locus_tag="U4A12_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1772661.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="U4A12_000247"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="U4A12_000247"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN1772662.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="U4A12_000248"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="U4A12_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN1772663.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="U4A12_000249"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="U4A12_000249"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN1772664.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="U4A12_000250"
     CDS             29970..30668
                     /locus_tag="U4A12_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1772665.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="U4A12_000251"
     CDS             complement(30836..33475)
                     /locus_tag="U4A12_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1772666.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="U4A12_000252"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="U4A12_000252"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN1772667.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="U4A12_000253"
     CDS             35318..36493
                     /locus_tag="U4A12_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1772668.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="U4A12_000254"
     CDS             36497..37138
                     /locus_tag="U4A12_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772669.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="U4A12_000255"
     CDS             37135..38052
                     /locus_tag="U4A12_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1772670.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="U4A12_000256"
     CDS             38049..38720
                     /locus_tag="U4A12_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772671.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="U4A12_000257"
     CDS             complement(39261..39749)
                     /locus_tag="U4A12_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1772672.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="U4A12_000258"
     CDS             complement(39950..40216)
                     /locus_tag="U4A12_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1772673.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="U4A12_000259"
     CDS             complement(40216..40479)
                     /locus_tag="U4A12_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1772674.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="U4A12_000260"
     CDS             40955..41140
                     /locus_tag="U4A12_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1772675.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="U4A12_000261"
     CDS             complement(41282..43105)
                     /locus_tag="U4A12_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN1772676.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="U4A12_000262"
     CDS             complement(43703..46408)
                     /locus_tag="U4A12_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN1772677.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="U4A12_000263"
     CDS             complement(46714..47661)
                     /locus_tag="U4A12_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN1772678.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="U4A12_000264"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="U4A12_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1772679.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="U4A12_000265"
     CDS             complement(48296..48658)
                     /locus_tag="U4A12_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1772680.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="U4A12_000266"
     CDS             complement(48669..49790)
                     /locus_tag="U4A12_000266"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN1772681.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="U4A12_000267"
     CDS             complement(49806..50156)
                     /locus_tag="U4A12_000267"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN1772682.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="U4A12_000268"
     CDS             complement(50286..51797)
                     /locus_tag="U4A12_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1772683.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="U4A12_000269"
     CDS             complement(52119..53471)
                     /locus_tag="U4A12_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN1772684.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="U4A12_000270"
     CDS             complement(53624..54700)
                     /locus_tag="U4A12_000270"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN1772685.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="U4A12_000271"
     CDS             54896..56641
                     /locus_tag="U4A12_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN1772686.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="U4A12_000272"
     CDS             complement(56921..57358)
                     /locus_tag="U4A12_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN1772687.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="U4A12_000273"
     CDS             complement(57342..58715)
                     /locus_tag="U4A12_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772688.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="U4A12_000274"
     CDS             complement(59037..59525)
                     /locus_tag="U4A12_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1772689.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="U4A12_000275"
     CDS             complement(59547..60149)
                     /locus_tag="U4A12_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1772690.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DASJOX010000005        56016 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJOX010000005 DASJOX010000000
VERSION     DASJOX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.005"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(137..1528)
                     /locus_tag="U4A12_000276"
     CDS             complement(137..1528)
                     /locus_tag="U4A12_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN1772691.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="U4A12_000277"
     CDS             complement(1741..2127)
                     /locus_tag="U4A12_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN1772692.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="U4A12_000278"
     CDS             complement(2183..2806)
                     /locus_tag="U4A12_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN1772693.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="U4A12_000279"
     CDS             complement(2870..3538)
                     /locus_tag="U4A12_000279"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1772694.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="U4A12_000280"
     CDS             complement(3550..3990)
                     /locus_tag="U4A12_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772695.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4750)
                     /locus_tag="U4A12_000281"
     CDS             complement(4049..4750)
                     /locus_tag="U4A12_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772696.1"
                     /translation="MKFYSKHQTFFSKYAILKDTLIERSTFLYVQLQENKTLLHFSPE
                     FHLEGLTLGEIYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMID
                     TNDGKIHLTAAELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLK
                     KYPGLLHTSIQFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFT
                     RNRNVLHQVAMEKAN"
     gene            complement(4858..5256)
                     /locus_tag="U4A12_000282"
     CDS             complement(4858..5256)
                     /locus_tag="U4A12_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN1772697.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5253..6026)
                     /locus_tag="U4A12_000283"
     CDS             complement(5253..6026)
                     /locus_tag="U4A12_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1772698.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6023..6706)
                     /locus_tag="U4A12_000284"
     CDS             complement(6023..6706)
                     /locus_tag="U4A12_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1772699.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6873..7760)
                     /locus_tag="U4A12_000285"
     CDS             complement(6873..7760)
                     /locus_tag="U4A12_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1772700.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7757..8626)
                     /locus_tag="U4A12_000286"
     CDS             complement(7757..8626)
                     /locus_tag="U4A12_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1772701.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8763..10049)
                     /locus_tag="U4A12_000287"
     CDS             complement(8763..10049)
                     /locus_tag="U4A12_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1772702.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10322..10900
                     /locus_tag="U4A12_000288"
     CDS             10322..10900
                     /locus_tag="U4A12_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1772703.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10900..11829
                     /locus_tag="U4A12_000289"
     CDS             10900..11829
                     /locus_tag="U4A12_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1772704.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11826..13562
                     /locus_tag="U4A12_000290"
     CDS             11826..13562
                     /locus_tag="U4A12_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1772705.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13625..14461)
                     /gene="pheA"
                     /locus_tag="U4A12_000291"
     CDS             complement(13625..14461)
                     /gene="pheA"
                     /locus_tag="U4A12_000291"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN1772706.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14458..14967)
                     /locus_tag="U4A12_000292"
     CDS             complement(14458..14967)
                     /locus_tag="U4A12_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN1772707.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(14960..16255)
                     /gene="aroA"
                     /locus_tag="U4A12_000293"
     CDS             complement(14960..16255)
                     /gene="aroA"
                     /locus_tag="U4A12_000293"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1772708.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16268..17359)
                     /locus_tag="U4A12_000294"
     CDS             complement(16268..17359)
                     /locus_tag="U4A12_000294"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN1772709.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17376..18542)
                     /gene="aroC"
                     /locus_tag="U4A12_000295"
     CDS             complement(17376..18542)
                     /gene="aroC"
                     /locus_tag="U4A12_000295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN1772710.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18544..19608)
                     /gene="aroB"
                     /locus_tag="U4A12_000296"
     CDS             complement(18544..19608)
                     /gene="aroB"
                     /locus_tag="U4A12_000296"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN1772711.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19641..20663)
                     /gene="aroF"
                     /locus_tag="U4A12_000297"
     CDS             complement(19641..20663)
                     /gene="aroF"
                     /locus_tag="U4A12_000297"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN1772712.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21322..22296)
                     /locus_tag="U4A12_000298"
     CDS             complement(21322..22296)
                     /locus_tag="U4A12_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN1772713.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22277..24160)
                     /locus_tag="U4A12_000299"
     CDS             complement(22277..24160)
                     /locus_tag="U4A12_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN1772714.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24178..26403)
                     /locus_tag="U4A12_000300"
     CDS             complement(24178..26403)
                     /locus_tag="U4A12_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1772715.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26518..27552
                     /locus_tag="U4A12_000301"
     CDS             26518..27552
                     /locus_tag="U4A12_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1772716.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27619..28632)
                     /locus_tag="U4A12_000302"
     CDS             complement(27619..28632)
                     /locus_tag="U4A12_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1772717.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28710..28898)
                     /locus_tag="U4A12_000303"
     CDS             complement(28710..28898)
                     /locus_tag="U4A12_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN1772718.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29322..29450)
                     /locus_tag="U4A12_000304"
     CDS             complement(29322..29450)
                     /locus_tag="U4A12_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1772719.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29453..30826)
                     /gene="rlmD"
                     /locus_tag="U4A12_000305"
     CDS             complement(29453..30826)
                     /gene="rlmD"
                     /locus_tag="U4A12_000305"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1772720.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(30937..31992)
                     /locus_tag="U4A12_000306"
     CDS             complement(30937..31992)
                     /locus_tag="U4A12_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1772721.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32002..33432)
                     /gene="gatB"
                     /locus_tag="U4A12_000307"
     CDS             complement(32002..33432)
                     /gene="gatB"
                     /locus_tag="U4A12_000307"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN1772722.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33429..34901)
                     /gene="gatA"
                     /locus_tag="U4A12_000308"
     CDS             complement(33429..34901)
                     /gene="gatA"
                     /locus_tag="U4A12_000308"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN1772723.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34901..35206)
                     /gene="gatC"
                     /locus_tag="U4A12_000309"
     CDS             complement(34901..35206)
                     /gene="gatC"
                     /locus_tag="U4A12_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN1772724.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35312..37348)
                     /gene="ligA"
                     /locus_tag="U4A12_000310"
     CDS             complement(35312..37348)
                     /gene="ligA"
                     /locus_tag="U4A12_000310"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN1772725.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37365..39599)
                     /gene="pcrA"
                     /locus_tag="U4A12_000311"
     CDS             complement(37365..39599)
                     /gene="pcrA"
                     /locus_tag="U4A12_000311"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN1772726.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39754..41313
                     /locus_tag="U4A12_000312"
     CDS             39754..41313
                     /locus_tag="U4A12_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN1772727.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41424..43355)
                     /locus_tag="U4A12_000313"
     CDS             complement(41424..43355)
                     /locus_tag="U4A12_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1772728.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43373..44287)
                     /gene="pfkB"
                     /locus_tag="U4A12_000314"
     CDS             complement(43373..44287)
                     /gene="pfkB"
                     /locus_tag="U4A12_000314"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1772729.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44284..45036)
                     /locus_tag="U4A12_000315"
     CDS             complement(44284..45036)
                     /locus_tag="U4A12_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1772730.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45195..45404)
                     /locus_tag="U4A12_000316"
     CDS             complement(45195..45404)
                     /locus_tag="U4A12_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772731.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45415..46743)
                     /locus_tag="U4A12_000317"
     CDS             complement(45415..46743)
                     /locus_tag="U4A12_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772732.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            46879..47034
                     /locus_tag="U4A12_000318"
     CDS             46879..47034
                     /locus_tag="U4A12_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772733.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(47108..47695)
                     /locus_tag="U4A12_000319"
     CDS             complement(47108..47695)
                     /locus_tag="U4A12_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN1772734.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47778..49028)
                     /gene="clpX"
                     /locus_tag="U4A12_000320"
     CDS             complement(47778..49028)
                     /gene="clpX"
                     /locus_tag="U4A12_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN1772735.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49275..50564)
                     /locus_tag="U4A12_000321"
     CDS             complement(49275..50564)
                     /locus_tag="U4A12_000321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN1772736.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50718..51650)
                     /locus_tag="U4A12_000322"
     CDS             complement(50718..51650)
                     /locus_tag="U4A12_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772737.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51807..52466)
                     /locus_tag="U4A12_000323"
     CDS             complement(51807..52466)
                     /locus_tag="U4A12_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1772738.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52469..53494)
                     /gene="sppA"
                     /locus_tag="U4A12_000324"
     CDS             complement(52469..53494)
                     /gene="sppA"
                     /locus_tag="U4A12_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1772739.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53507..53797)
                     /locus_tag="U4A12_000325"
     CDS             complement(53507..53797)
                     /locus_tag="U4A12_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772740.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54206..56011
                     /gene="glmS"
                     /locus_tag="U4A12_000326"
     CDS             54206..56011
                     /gene="glmS"
                     /locus_tag="U4A12_000326"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN1772741.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT        15643 a        12068 c         9961 g        18344 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaatagcat attttgaaaa gaaagtctga tgcttagagt
     4741 agaatttcat aaaaatttac tatttcataa gtattcacga gaaaaatgca aacaaaaaag
     4801 cggaaataac gctcatccgc cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta
     4861 tgattcagac ttttctctat cgttattttc atctaaaagt tcttgcattc cgataatttc
     4921 tcgatattga tgaatcttag tgataccaat gactttgtct acatcaatac taaaacaaat
     4981 accattacct ggaatatcga tgttgatgcc ttgaccaatc gcatccatta ctttattcgt
     5041 caaactatcg ggtactaatg tcaacacaat atctttttct ggcgaaatgt ccaaacctaa
     5101 aaatttccct gaatcatgta caccagaacc tcttccatgt aagatcgttc cgcctgctgc
     5161 acctgcttct tttgaaaagt cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac
     5221 aatcatgtga agattcaatg ctacaagatc tttcatttgc tttcccccaa tttttgcaat
     5281 ctttcttttt cgattctgtc tttttcatct tttcgtttat aaatatatcc tagtactaaa
     5341 gtggataata ttggtgtcaa tgcaatcatt gcaaccacac caaacccgtc aatgatcgga
     5401 tctcttcctt caatttgtgc agcgattgcc acgctcattg ataaaataaa tgtcgaacac
     5461 atcgggcccg ttgctactcc accgttatca aaagccattg ctaaaaagat cttgtccact
     5521 tttcgtccta aaataaatgc tagcccgtat cctgggatca agaaataata aagagagaat
     5581 ccgacgagca aacgccacat agacaatcct actgctgcgc cgatcccaat tgaaattacc
     5641 atcagcatga ttttcgatcg gattctcccc tcgctcaatt cttcaacctg tttgatcatt
     5701 acatgaatcg ccggctcagc aaaccctact aagaacccca ttaaaaagcc aaggggaacc
     5761 agtatataat tgtaaggcaa tcccgcaata gcactgccta aatgctgtcc taccggtaca
     5821 taagccatat tgactccgtg caggaaaagg atcaacccaa tagtcgttat caagaaacct
     5881 ttcataatgt caaagaaacg ctttttgcgc atacgaaaac taatgatatt cattaaaaca
     5941 aataggatta cgataggtgc cactgccaaa atgacttcca tggtaacgat ttcgattcca
     6001 tctatcactt gctgccagaa actcattgga agatcacccc caaaatcatc acggcaagaa
     6061 tcggccctaa cgaagcaata ccgaccattc caaaactgtc attttcattt ttcttttgtt
     6121 tgatcatact agtcatccct ccggcaaggg ccaagataaa tggaacagtt actggaccag
     6181 tagtcactcc ccccgaatca aaagcaatcg gcataaattc ggaactggtg aaaaatgagg
     6241 cgatcaaaac gcctatgtag cctatagcca tcaattgata ataggatagc ttgaatacga
     6301 cccgaagcag cgcaaaggct agaaatactc cagtgccgac gctgacgatc ccaatcagca
     6361 acactctgcc aatctctccg tcagatatat catatacttg ttgtcccaaa acttgcacgg
     6421 aaggttcagc aatcgtgatc acgatcccga tagcaaatcc caaactgacc aaaacggtca
     6481 aacttttctt tttgatcata tatttcccta ctagatctcc gacttccatc atagaataat
     6541 ctgctccaaa caaaaagagc gtcattccga tcatcatgat tgccgcacca accagaaatg
     6601 aagcgaggtc ttctccttct aacggtgcaa aaacaaacgt taaaatgaca atcaggacag
     6661 tcatcggcaa tatagcagca atcacttctt taaaattttt tagcatgtat cctcctcctt
     6721 tactctttaa gtttacctta aaatgaaaga gttttcgagc acaaacttaa aacgttatca
     6781 tttaattttt ctcaacataa aataacacac gtcccaaaag tatcttttta cttggaaaaa
     6841 tgactttgaa ggaacgcgct tattgtgttt ttttatttta cagagccgat cattccttga
     6901 acgaattgct tttgcaaaac aaaaaatacg atggcagttg gtaaagtagc gatgactaat
     6961 ccagccatga tcattccata gtctggtgta taggaagcac ccatagcaga taaaacaagc
     7021 ggaaccgtcc gtttatcagg agattgcaaa gctactaagg gccataaata attgttccag
     7081 ctactcatga atgtgatgat tgcagccgcg gcataggtat ttttagccgt cggcatatac
     7141 actctaaaaa aaatacttag ttcttttaga ccgtctaatc gagcggcttc tactagatct
     7201 tttggaaaag atttaacatt ttgtcgaaag aagaagatca aaaaagcagt gctaacagat
     7261 ggcaggatga caaccaagaa actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac
     7321 attcgatata aagggatcat caatgccgca aaaggaacca tcatcgatag cagtagaata
     7381 ttgaaaatta ggtcttttcg tttactttga taaatctcaa atccatatcc tgccattgac
     7441 gaaatcagca atgcaaacac agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa
     7501 cgagtaaaat tcagctcatt ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca
     7561 atctttattt ttcccgatgt gatatccacg ggtgtattcg tcattcccaa aaccatccag
     7621 acaaaaggga aaatggaaat aaatgatagg atgcttaatg ctgtatactt taatactgac
     7681 ccaattgtcg aacgaagttc taatgcttgt tttttgacgt tggggcgttc gattgtttct
     7741 ttttctacca tatttttcat cgactttctc ctcctacttt cgtttggatt atcgacaata
     7801 tcacaataaa ggaaacaata acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga
     7861 atttataact caaattatag atgtattgag atagggtggt cgttgcattt gctggtcccc
     7921 cgccagtaat attttgtact tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg
     7981 aagtaaacaa aatgattggt tttaaatttg ggatcgtcac tttgataaac tgctgccatt
     8041 tagaagcacc gtcaatagat gctgcttcat aaatctccga atcaatattt tgcatgcctg
     8101 aaaggaaaaa aatcatattg tatccagtcc aacgccaagt gatcgaaaga atgatcagaa
     8161 ctctcgccca aattggatga gtcaaccagc caatcggtgt gtcaatcata ccaatgccca
     8221 acaacaagtt gtttacaagg ccagattgag aaaatagact tttcatgatc agagaataac
     8281 tgaccatcga agtgatacat ggaagaaaga tagcggttcg aaacaaccct ttatatttca
     8341 actttttatc atttaaaagg ttggaaataa aaagtgctag aaacaacatg atcggtactt
     8401 gtacaagtaa atacaggaat gtattaaaaa aagctttttt aaaagttgca tctgtcaaca
     8461 tccgctgata attacctaac ccgttaaatg tcatattgac agggggacca ctttgaaaag
     8521 acgtgatcaa agccaaaacc atcggaataa aaaccatgat tgatatcaaa atgactggca
     8581 agattaaaaa agcatgcccg tttttgaata aatctttctg cttcattctt ttccctccaa
     8641 ttctatcatt gattatctac atgaaaagac aagaaagaca atatctgtaa ggcagacgtg
     8701 atcttttttg tcttttcgtt tgtctgatga aaaagcgaag aaattcactt ttttaccacc
     8761 gatcaattag ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc
     8821 tcttctccat tgatgattcg atgaagagct tcagctacga ctgcttcaat tgcataggtc
     8881 tcatgtccgt aattcacttc tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc
     8941 ttttgaccac tgtaaaattc agaagcttct tgataagctt cttgttcatt cgcgctaagc
     9001 atcgtagata ctagtccgat ttctttcgca agagtgccca tcagttcttc attcgaagca
     9061 aacgttgctt ctaggaaatc tttcgctgaa tcttctccag ctactccttt gatcacatac
     9121 cagccagcgc cgccaaggtt agaagcttgc gctttttgca gattttctgg aagtgcaggt
     9181 attggtgcaa tcttccattt tccagattgg ttttctgctc cttggatcgt tgaagaatac
     9241 caagcgccag ttgggctaga agctactgct cctgattgta tcgcagttac tccagcattc
     9301 cagtcagagg tttgttcgac tagatcttct ttcaaaagag tggcaaacaa ttcaagacca
     9361 tactttaaag atgcattgtc ttggatcgtg actgtctccc catcttcaga tgtataccat
     9421 tctcctgcct gttgcataat catccttact cgaccaaggt cagaaggatt gacttcagcg
     9481 attttctttc ccgtcttttc tttgacatct cgcgctactt gaatatactc gtcccacttc
     9541 agattctcca tatcctcttc tgtataacct gcttctgcca tcagatccgt acgatagaag
     9601 tttgctgtta cgcccgaatc gaaaggaaca ccgtatattt tatctcctac tttgtttacc
     9661 gcaaatttat acggtgcaaa atgatcttcc tttacaatag cagacaaatt agcgaatgca
     9721 tctggataag aagtcaaata gccttgtatc ctataatctt caatcaagac gatattgggc
     9781 aatccttctg tattcccgct cgctaaagcg gtgttcagtt tctgaacgat atcatcctga
     9841 gacatcgtaa caacttctat ctctgtttct tcgttctcat aaactttttt tgcttcattc
     9901 actgctttga tattgaatgt ctcatcccac gcccagacag tgactttttc ttctttgtct
     9961 gctgtactac tagcacttcc gcatcctgtt aagaaacctg atgctgccgc taaacaaacg
    10021 actccagcaa ttaactttct gatgttcatt tttatccgct ccttttccat ccattagttg
    10081 tttacgtttg ctacattcat cataccaaaa tttgaaaacg gtttctttat tattcagata
    10141 cttcaaaaat ttctcctaaa ataataaaaa aatacaaatc aactatgaca aaaggaataa
    10201 tcttagcatc tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa aatatccttg
    10261 cattttctcc tagaaataag tatttttaaa ttacaagact attcttaagg cgggtaaaaa
    10321 aatgccaata cgcgtaattc ttatcgatga tgaacagacg attttagatg gaatgaaaca
    10381 gttgatcaat tggtctgagc acgggtttca attaataggg actttttcca gtccgatgaa
    10441 agcgcttgat ttttctttgg tacatgcaat cgatatcgtg gtcacagatg taatgatgcc
    10501 tgaattaaat ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat
    10561 ccttatcctc tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt
    10621 cgctgattat cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc
    10681 cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca
    10741 gatctgcgag aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct
    10801 tctttttgaa cacaccttgt ttttctgtta tataccgaaa aacggccatt cagttcaaaa
    10861 caaggctcta aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt
    10921 actatttatc ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc
    10981 aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt
    11041 tttgttgttt ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga
    11101 aattgcacca aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa
    11161 cttcttccgt tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt
    11221 cataaagact acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa
    11281 cagcctattg atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc
    11341 aacgctttgg ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt
    11401 ccttctttat tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca
    11461 cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact
    11521 ttgatctacg agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc
    11581 gaaaaatatc atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc
    11641 aacttttcaa aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa
    11701 acacggctta ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc
    11761 tccagagttt ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt
    11821 gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt
    11881 tcatcggtct ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg
    11941 atgcctatat ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg
    12001 aatttgaaag tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag
    12061 ctgtcaaaag ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa
    12121 gaacaatcca aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg
    12181 tattgatctt tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat
    12241 ctgccgattc tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata
    12301 cacaaatgct ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct
    12361 tctttgtcaa aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga
    12421 tctcatccaa gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga
    12481 tctccttcat gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga
    12541 gccgatcaat gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc
    12601 agttgacccg tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc
    12661 attctttatc taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac
    12721 ttgcttttgc tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc
    12781 gcttgattga ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac
    12841 aaggaagcca tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc
    12901 agcatcatta tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa
    12961 ctatgcaagc acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt
    13021 tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac
    13081 taagaagtac gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg
    13141 tacaaagtta catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa
    13201 tgattcctga tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg
    13261 aaaacgccta tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca
    13321 aaacaaaaaa tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg
    13381 aaaataccaa acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc
    13441 ggatacgcct aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag
    13501 gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat
    13561 aaagaatagc caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg
    13621 actttcatct acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt
    13681 ctcctccgag catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca
    13741 acaacaaatc gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa
    13801 tcttacttaa gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg
    13861 gccgattagc aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg
    13921 tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat
    13981 tctcagctaa gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg
    14041 cgtcttcttt cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta
    14101 ctaaaggaac attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg
    14161 gatgtgaaag gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag
    14221 gaagaacgat ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt
    14281 gatgaacagc tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc
    14341 gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa
    14401 atagctgaca agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca
    14461 tatgcttttg atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt
    14521 gttgattcga tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt
    14581 tatttcttct cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat
    14641 acgatcaact agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa
    14701 aaaacgccga ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc
    14761 atttgacagc tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata
    14821 gtgaaaatat tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac
    14881 atactctttt tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat
    14941 gaaaccaatc agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat
    15001 cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc
    15061 catcagctat caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg
    15121 acacttttgc gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt
    15181 ttatctttgc tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt
    15241 ttacttttaa ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta
    15301 aagctagaat gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt
    15361 tcttcaaatg actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg
    15421 tctgggtaat cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat
    15481 tgaccccgac attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa
    15541 agaaagcagc tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg
    15601 gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca
    15661 tttcttccgt atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt
    15721 gtaatgccgc aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc
    15781 ggataggtga taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt
    15841 ctccttgcaa gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt
    15901 tagacaacga agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca
    15961 tcaataaacg tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa
    16021 atgagtggct accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac
    16081 ctaattgttg gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta
    16141 tagtcgtcgt tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat
    16201 ctgctggtat cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc
    16261 cagcctccta tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt
    16321 gtaacaaatc ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg
    16381 tctcttggat cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt
    16441 ctgcaataac gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg
    16501 cgccttgctt atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa
    16561 agatttccga ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc
    16621 aatctgccaa tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga
    16681 caggatctga ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg
    16741 ctcgcggata ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat
    16801 gtggaaaatg acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat
    16861 attttgcttt ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt
    16921 ccaaaggtgt aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac
    16981 ctgatttatg agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag
    17041 cggatgtcac gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca
    17101 agttgattct ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata
    17161 agataacgtc tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct
    17221 gctgttctac tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt
    17281 ctggattggt ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga
    17341 taaaaacatg ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg
    17401 tactccttca ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc
    17461 tctgtcgcta ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct
    17521 gtactatccg aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt
    17581 tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg
    17641 ccattggtca ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt
    17701 tcttcttccc ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat
    17761 ccgaccccaa attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg
    17821 gcatctagct tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg
    17881 agcacttcta caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct
    17941 tttattcttt gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct
    18001 ttgatttctt gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc
    18061 acgtgtcctg ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt
    18121 gctacacgca tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga
    18181 tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc
    18241 cgcagtgatt ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc
    18301 caatctttat tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg
    18361 ccagacgtga tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca
    18421 gcttgtctgc gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt
    18481 agtccttcaa taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc
    18541 atcttacagt cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc
    18601 ttgcccaatc ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg
    18661 ggttatcgcg tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa
    18721 tttttgaagt atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc
    18781 tgcatgagaa gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc
    18841 atatcctgct gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg
    18901 atttccttgg tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt
    18961 aatgattgga acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt
    19021 ccaaagcgtt gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa
    19081 acgacgtacc ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct
    19141 aaatgttccg acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact
    19201 agaatctact tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa
    19261 cgtcgaggcc acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc
    19321 atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa
    19381 agatttactg gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct
    19441 caattggaac atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc
    19501 tactttttga ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt
    19561 ttcaatatga atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc
    19621 tttcttttaa aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg
    19681 atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct
    19741 gaccaagcat ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc
    19801 acacccgcac gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca
    19861 acaatcacag ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta
    19921 ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg
    19981 tcttcgactg caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca
    20041 cgtttcaatc caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc
    20101 atattacgtg cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct
    20161 tcatccatca cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat
    20221 ttcaatcctt cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca
    20281 ccacctcgca gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg
    20341 tctaatcctt cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt
    20401 actccgtcta catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat
    20461 gtgtttaaaa tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt
    20521 aaacttcgcg tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag
    20581 actttgaatc cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct
    20641 tcttttttca taattactat cattgttttt cccgctttct tttgtttact tatctatctt
    20701 ggctgatttt ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt
    20761 cctgttgttc tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa
    20821 agatgtccct gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc
    20881 tgcaaaaata taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt
    20941 aaaaatcttt agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat
    21001 acaaacctat ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt
    21061 cataaaaaat cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg
    21121 actgtcaatc atttttatga aacttttttt aattttctga tagttaactt tgtttttcag
    21181 aaaaataaca attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt
    21241 tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt
    21301 caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct
    21361 gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct
    21421 tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt
    21481 tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg
    21541 ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt
    21601 tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta
    21661 tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc
    21721 cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat
    21781 gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt
    21841 gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac
    21901 tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata
    21961 ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat
    22021 ttctttgact tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc
    22081 gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag
    22141 ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata
    22201 atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc
    22261 cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg
    22321 cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg
    22381 gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca
    22441 cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat
    22501 caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt
    22561 cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg
    22621 gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat
    22681 tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc
    22741 gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt
    22801 tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta
    22861 ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg
    22921 attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag
    22981 tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag
    23041 ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat
    23101 gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga
    23161 tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt
    23221 ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat
    23281 tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga
    23341 tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct
    23401 caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga
    23461 tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc
    23521 caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct
    23581 ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga
    23641 acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta
    23701 caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg
    23761 tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct
    23821 cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat
    23881 tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat
    23941 caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg
    24001 gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta
    24061 cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa
    24121 aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca
    24181 taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag
    24241 tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag
    24301 tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag
    24361 tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc
    24421 aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg
    24481 aatcgtttga taggatataa tttggtcttt tacttgcttt ttttcttcag gagacagctt
    24541 tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa
    24601 cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga
    24661 aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt
    24721 atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc
    24781 accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc
    24841 ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat
    24901 cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt
    24961 atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc
    25021 cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc
    25081 aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc
    25141 aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt
    25201 gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca
    25261 gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt
    25321 gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg
    25381 ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg
    25441 actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga
    25501 ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa
    25561 ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag
    25621 tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt
    25681 catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga
    25741 ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa
    25801 tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc
    25861 tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt
    25921 atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg
    25981 tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc
    26041 tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat
    26101 gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt
    26161 atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct
    26221 tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc
    26281 caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa
    26341 gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc
    26401 catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt cctgtatact
    26461 gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg
    26521 gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg
    26581 ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca
    26641 gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg
    26701 attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat
    26761 ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat
    26821 aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt
    26881 gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc
    26941 gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg
    27001 atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc
    27061 gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag
    27121 aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat
    27181 catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat
    27241 gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat
    27301 cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat
    27361 aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa
    27421 tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa
    27481 ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca
    27541 aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa
    27601 cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca
    27661 attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt
    27721 atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc
    27781 ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc
    27841 cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt
    27901 tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga
    27961 atgctgctgt gctgttagct tcaaatcaac aggtccccaa taagaaatgg ttcctttcag
    28021 actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg
    28081 tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg
    28141 ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt
    28201 cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat tcatcgcaac
    28261 cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc
    28321 tccgccgacg aatcctccac catgtaccca aaatagaaca ggaacaggcc ccttgctgtt
    28381 ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt
    28441 ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt
    28501 atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag
    28561 aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat
    28621 tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg
    28681 ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat
    28741 gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt
    28801 gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct
    28861 tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc
    28921 aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc
    28981 acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt
    29041 ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac
    29101 atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca
    29161 gttaatcaaa ttgaaataac gaaaaatctg agccagaatg aggttcattc tgacccagat
    29221 ttgattttgt acaacccatc agtccttatt gacaaagagc cactaaaaag actaagaaac
    29281 aataacaaaa gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta
    29341 gggccttttc tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct
    29401 ccaccttgtt tctttttgat caattctttc attttttctt tttggtccat ttttattctc
    29461 ctttaacgaa tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct
    29521 gaactttctt cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg
    29581 ccggattaca tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa
    29641 aatgatgatc taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt
    29701 tttcttgcca ttttttcata actttttctg cttttccgac ttcataatga acattttcga
    29761 ttttattatt taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc
    29821 catacacctt atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag
    29881 cgtcgatcac aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat
    29941 actctgcttg ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc
    30001 ctaatagctg atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa
    30061 tcacatttgt tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt
    30121 catgaatagc ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa
    30181 cgaccatcat ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt
    30241 catttttttc ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt
    30301 tgattgcttg atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat
    30361 ggctattctt tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct
    30421 gtgctttatt acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt
    30481 ctggcatctt agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct
    30541 gttgttcata cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt
    30601 ctacaggctg acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact
    30661 tattgcctgc ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa
    30721 ataatggata accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt
    30781 cgactgtata tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta
    30841 agcctatttt tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc
    30901 ggcactgacc ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc
    30961 gtctgctatt tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac
    31021 gaattctttg ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg
    31081 aatctgatcg gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg
    31141 tgcttccccg ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc
    31201 tgtttcaacg aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg
    31261 accgccattc agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac
    31321 aatgattaaa gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc
    31381 tccaacagag ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc
    31441 atcatacttc aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc
    31501 aagataagca agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc
    31561 tgtcaaatag ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc
    31621 catttttacg gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg
    31681 aatttttaat gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg
    31741 acggtgttca agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc
    31801 tgccactact agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc
    31861 tggttctggt gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag
    31921 gatatccgcc aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg
    31981 tgcttttttc attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac
    32041 gacaccagga tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac
    32101 cgcacgatct tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc
    32161 attaatgatt ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac
    32221 tacttcttgg gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt
    32281 tgagctgatc gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa
    32341 ttttgtctca gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa
    32401 ccagttagat gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga
    32461 catttctttt gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat
    32521 atagcgttcg cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca
    32581 ttcgtcatcg atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga
    32641 cccttctttg acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc
    32701 ttgttggatt tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa
    32761 accttttttc acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc
    32821 ttcttgtcca taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat
    32881 tttcacatcg ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta
    32941 tgcttcttct ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg
    33001 tcggttcaaa tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc
    33061 ttctaaatgg acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat
    33121 ccatccgtca tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc
    33181 tgggtaaaag taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc
    33241 caaagctgct ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg
    33301 ataaccccag tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc
    33361 tggagagaaa atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt
    33421 ttcaaaattc attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt
    33481 gcttgttcaa acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg
    33541 atctgcaatc caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt
    33601 ccagccaagt tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta
    33661 atgttctcac cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa
    33721 tctgcaaaga ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg
    33781 aagtaagcgt cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt
    33841 acttcttcac cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca
    33901 ttttcagagc gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct
    33961 gatgaggcaa tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct
    34021 tccacaacag cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg
    34081 ccaggggcca caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg
    34141 tcgcctgtca gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt
    34201 tcgtcataac cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt
    34261 ggtccgattt ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca
    34321 taagtaggtt tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca
    34381 gtatcactac ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca
    34441 gaagagccac caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa
    34501 gtttctgtac ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata
    34561 tctgcttgat agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg
    34621 atcttagatg cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt
    34681 ggaataccag caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt
    34741 tccaatgctt tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg
    34801 attcgttcaa atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata
    34861 tgatgcagtt cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat
    34921 tatcgatgat tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata
    34981 attcatcgcg gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa
    35041 ccacattcga cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat
    35101 cgatgatttt acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt
    35161 ttgctaattt ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt
    35221 ctaaacgagt taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt
    35281 ttattgaaat ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca
    35341 atgcatctgc catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca
    35401 gcttgctgcc agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag
    35461 tcactttccc gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta
    35521 gtttaccagt aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg
    35581 catttcgaag ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt
    35641 catggacttc ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg
    35701 tatcaagcgc aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat
    35761 gttctgctaa aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca
    35821 accgctcgac cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt
    35881 tttcttttat tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa
    35941 catctgctac tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga
    36001 tattcatcgc atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt
    36061 taggattgat gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa
    36121 ctggacaatg tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga
    36181 cttgcgaaac ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc
    36241 ggatatcctt caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc
    36301 ctgctactcg gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg
    36361 tccattcgat gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg
    36421 cccatctcgg tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt
    36481 tgatgacgat tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa
    36541 tgtatgacca cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc
    36601 caattttttc tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat
    36661 cagctactgt gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact
    36721 gacgcaaaga accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt
    36781 ctctgtcttg gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt
    36841 cgatgttcat tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag
    36901 tgatgttttc tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt
    36961 cataacgcaa agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac
    37021 gtcccactgc tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt
    37081 cgttcaaact gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc
    37141 cgcctactcg ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg
    37201 ctactaattc tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta
    37261 cgtagtattc gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag
    37321 cttcgtcaat cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt
    37381 ggcgcaaagg cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct
    37441 agatccttcg acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg
    37501 actttatctc caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca
    37561 accgctgtct ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt
    37621 ttaggcgtag gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa
    37681 cgagaaggtc ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa
    37741 tacaacgctt cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt
    37801 tcgctttctt ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg
    37861 aatacaactg gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa
    37921 gcatcttctt ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt
    37981 agtttttctt ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat
    38041 tcttgtgtga ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta
    38101 ttttggattt ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct
    38161 gtcactgtca agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct
    38221 aattgctgtc ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc
    38281 agtggccatt catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg
    38341 cctcgtttgg gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc
    38401 acattcaaat acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca
    38461 accattttat aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg
    38521 ttcgtccggt acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct
    38581 tgcatccggt ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg
    38641 atcttgtttc cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt
    38701 tcaatgactt ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc
    38761 aaaattactg cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca
    38821 ccgcgccagc cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta
    38881 cctgccaata aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca
    38941 tgaatataat ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat
    39001 cgaatcgtat tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt
    39061 tggtaagcag cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca
    39121 aaatcctgag gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga
    39181 ggatcatatt ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc
    39241 tgttcagaag aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt
    39301 cttaaaatac gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat
    39361 gcgagaatcg cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc
    39421 gctaggatgt tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc
    39481 agcactctcg tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga
    39541 agaacagctt ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt
    39601 aacaacatcc tttcttttat caatcttgtt aattatagca ttttcttggc tttcattaaa
    39661 ctagctagtt caaaatcatt tgtttctttt tatggatttt tccttgtcaa ttcgttgatt
    39721 tcgcgctaaa ttaaaagaaa gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa
    39781 ttagataaga ctcagatata ttttattcta aaaggaatat ttgttggact gctgacagga
    39841 atcgtcgtta gcctgtttcg attatctatc gaaaagctaa gcgatttagt tcgaagtatc
    39901 tatgaaatga gtcgggaaca gccgatttac ttaattggcc tagctatttg ctgcatcctt
    39961 gcagcatttt tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt
    40021 ccacaagtag agggacagct tcgcggggaa ttatcaatga actggttctc tgttttatgg
    40081 aaaaagttca ttggcggtgt actttcagtt ggtgcgggcc tttttctagg tagagaagga
    40141 ccttctatac agcttggcgc atccattgga caaggagctg gacaattgtt tcggagtcca
    40201 tcttcagaag aaaagatttt aatttctagc ggtgcaagta gcggacttgc agcagcattc
    40261 aatgctccta tcgcaggatt actctttgtt ttagaagaaa tccatcactc cttttctcca
    40321 cttgtttggc tgacatcatt tgcttcagca atcactgcta acttcgtctc tctttacttt
    40381 ttcggcttga gacctgtttt atatctaggt gaccttcctt ctttgccgtt gaaatattat
    40441 ggttccttag tgatcttagg agtacttcta ggcatcttag gttttattta tcagaatgtc
    40501 ttgctggctt tgccaaaatt ttacaaccga ttgcctttac cagcttattt tcacgggttc
    40561 gttcctttct tgctgatttt gccaattggt tatttatggc ctagtactct aggcggaggc
    40621 aatcaaatcg ttttagcttt tggacatgct tccttgccaa tcattatgat tatcggtttg
    40681 tttgttcttc gattcgtttt ttccatgatt tcttacggtg ggggtcttcc aggggggatt
    40741 ttccttccga tattgacatt gggggcttta atcggtagct tttatggaaa cattatggtt
    40801 gatttcggga tggatccaat ctacatcaag gattttgtcg ttattgccat ggctggttat
    40861 ttcacagcga ttggaaaagc accgctgaca gcaattattt tagtgacaga aatggttggc
    40921 tctcttaatc atctgatgcc tttaggattg gctgctcttg ttgcttatat cgtcaatgac
    40981 cttctcggcg gtaatccaat ttacgaatca ttattggaac gtctacttgg aaatactcag
    41041 accgcctcac taaaaggtgc aaggacaact tttgattttc cagtaacggc agagagttct
    41101 ttagacggat taatggtacg agacttcaat tggcctaaaa atatgctgct aatctctata
    41161 cgcagaggaa atcaggaaat cttgactcac ggtgatacga tcatgaatgt aggagatatt
    41221 ttggttatct tgactgatga agcaaacttg ccaagaatca agcaggaaat cgaggctaaa
    41281 tcttctatca caaatttgaa gcaatcattt taacgaacat ggaaaaacta ttttacaaca
    41341 actattcatc tacactactt gattctatat gattctataa aaaaagaaga ggctgggaaa
    41401 acccagcctc ttctttttta tttctatgct tctgctactg caggagcttt tctcaataaa
    41461 ccataaatca caccagatac gactgctccg atagcaataa acaccaaata catgaccggg
    41521 tgactcagta agaatacaac gaaaatcccg ccgtgaggag ccagtaattt gattccgaaa
    41581 gctccgacta atccgccagt taaagcagaa ccaataataa aacttggaat ggcacgaatc
    41641 gggtcagctg ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc catcacgatg
    41701 ttcgtcaaac cagcatcttg atctgttttg ctgaatttat tcttaaacaa acgtgtagca
    41761 acgaaaattg ccaatggagg taccattcct ccagccataa cagaagccat cacgacactt
    41821 ccgccagttg caacagtcgt agctaaagtt gctgtaccaa acacataagc ggctttgttg
    41881 attggaccgc ctaaatctgc cgccatcatt ccagctaata atgcaccaag taaagcagca
    41941 ttcgaaccac tcatacttgt caagaaaccg ttcaatgcat cattgattgc tttcataggg
    42001 acattgacca gcaacatcaa gaaaccagta atcagtaagc caaacactgg ataaaagagg
    42061 atcgttttaa ttccatccaa tgattttggt aagttcttga ataatttacg taaaaatacg
    42121 atgacataac cagctaagaa tccaccaatc agtgcaccta agaatcctgc accgcctgta
    42181 ttagctaatg caccagcagc aaatccaacg atcaaaccag ggcggtcacc gatacttgaa
    42241 gcaataaatc ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc gatttggttg
    42301 aaccagctag cagcttgatt gtaattccct agatttgata attgatcatg tggcacgcca
    42361 ataaattggt cgatcatgaa agaaagcgca atagcgattc ctccaccaat aacgaaaggt
    42421 aacatgtgtg atacgccgtt catcaaatct ttgtaaattc tttgtccgat agtaccatcc
    42481 gcatttcctt cttctgaact gctttctttt ccatcaccat ggaatactgg tgcagtacca
    42541 ctaagggcta gattgatcaa ctcttctggt ttacggatac catcactgac aggacgattg
    42601 atcagttctt ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc aacgattaca
    42661 ccttctgcac gggcgatgtc ttcagcagtc aatcggtttt tgattccttc tgaaccgttc
    42721 gtttcgactt tgatctccac acccatttct ttcgcttttt tcttcaaagc atcttctgcc
    42781 atgtaagtgt gagcgatccc tgttggacaa gctgtaacgg caacaacgaa ttttctttct
    42841 ggatgagcag aagcttctgc agcttgtgtt tcttgttctg ctttttcttc tgcttctttt
    42901 tgctcttcag ctgtttggaa caagtcttgg acttcttctg gtgtatttgc ttcttttaat
    42961 tttcctacga aatctgcatc aatcagcaat cgagatagtg ctgctaatgc ttgcaaatgt
    43021 gtgtcgtttg cgccttctgg agctgcgatc atgaaaaaca gataagttgg ctgtccgtct
    43081 aaggcttcat aatctactcc tttttggctt tttgcgaata aaactgttgc ttctttaact
    43141 gcactgtttt tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga agtttgtgct
    43201 tcgcgggcta gaattccttc tttatatgtt tcgatatcgg aaatccggcc ggcatcaaac
    43261 attttttgga ccatttcatc gattgcgcct tttttatcag tcgcttgcaa atccatgatc
    43321 atcgcttctt tgaccattaa atccttgata ttcatgatta tcctccttga aactaatcaa
    43381 tttttgtgat ttcaacttct ggcagcaact catcaatcaa ttcgcgagct gccaagtcat
    43441 cagaaaaagc tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg attggatctt
    43501 tcgtacggct gtaagcccca ataaagccag caatcattga atcccctgca cctactgaat
    43561 ttttcaatgg gcgaactaat acattcgttt tataagttcc ttgttccgtg aagagcaaag
    43621 caccgtctcc agccattgag atcagcacat tctgtgcccc ttcagctagt aattttttcc
    43681 catacggaat gatttcttct acagattgga aggttgtgtt gtaaagctca gctaattcgt
    43741 gattgtttgg tttgatcaac aacggtttct taacaagtgc attttttagg tcatcacctg
    43801 ttgtatcaat cacgaaatct gcaccttttt ctcgaatgat atcaatcagt tcttgataaa
    43861 atccttttcg caggctagct ggcgcgcttc ctgacaatac gacgatatct ccttgtccta
    43921 ctgttcctaa tgcttgcttc aactcatcga tttcttcatc tgaaatagat gggcctaagc
    43981 cattgatctc agtttcagtg tctgatttta gtttgatatt gattcttgta tcttctttta
    44041 cggttgtgaa caaagactgg atttgttctt tttcaagcca cttcgaaata aattcgccag
    44101 tgaaccctcc taaaaaccct agtgctgtag attgagaatc gatccgtttc aaaatacggg
    44161 aaacattgat cccttttcct ccaggtaact tgaagtcttc cttcattcgg ttcaaatcgc
    44221 ctagttttaa cccatctacg cgtacgatat aatcaattga tggattaagt gttactgtgt
    44281 aaatcattgt attgcctcct taattgttgt cttttcacgg aattcattaa cgaattctaa
    44341 tggacaacgc tcggttatga tcgtggctgc ttccaaaggt gcaacagagg tgaaggacaa
    44401 ctggttgaat ttggtatgat caatcaatac aaatgtctct tcggaatttt tcatggctaa
    44461 acgtttaaca gctgcctctt ctggatccgg agtcgtgaat ccttgttcaa gatgggcacc
    44521 gttcattccc ataaatgctt tattaaaatg aaaatttttt agttgttcta tcgcattaga
    44581 tcctaaaaca gcattagttg ccagctttat cgttccacct aaaataatgg tagcaagctc
    44641 caaatcaact aaatttgccg catgttgaac ggaatttgtc actactgtga tctgttttcc
    44701 ttgcagaaaa ggaatcattt ctaacgtagt agagccagcg tctaaataaa tgacatcttc
    44761 gtcattaatg gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa cgtttttgag
    44821 tgatttttca ctcatattct gctcaaaatt caataattgc tctttcttag ctccgccatg
    44881 aactctttct agaaacccac tattctccag ctcttgtaaa tcacgtctta tcgttgattc
    44941 agaagcattc aaaaggttta caagatcttg tgatttcacg attttctgct gttcaagcaa
    45001 gtctaggatt ttttgtctgc gttcctctgt aagcattttc acgatcacct caatacaaat
    45061 aatagcatat tttttttcta ctagcaatat aaatcagtca aaaacgttca taaaaattat
    45121 aaatcagtca aaaaaaaata aaaaaccagt cagaaagact ggttttttat ttttttgtct
    45181 atcatcaagc cgttttatcg ttttttattt tcgcttcttg tttttttcat ataaacagaa
    45241 gcaggttttc tttccttatg aagaagataa taataaagtc ccatcccgcc aaacatgacc
    45301 aaaaaaccga tgactgatcg aataggaaaa ttaggaacaa aaaagagaca gacggataat
    45361 aatgatgccg gaaccattat tcgataatag agttgtttat ccattttctt cacctcatat
    45421 tttttgtaaa gtaatcaatt gttctaattc tcggtaatct aaaaattgca cacgcagatg
    45481 ctgctcacct ttcttgacat cgaattggat acttcgccgc tttttcttta ttcgtttgaa
    45541 ggtaacatca gagagtctat gtttgatttc ctttaacggt aattctgaca gggattctga
    45601 aggaacatag attcttaagg ataaaagata ctcttgatat ttttcatctg ttacctctac
    45661 ttctagatat tccctaatat aataacaata agcttcgatt ccattatcca aagcaacagt
    45721 cggatcgata actttatagc acatcatgcg tgatcgattc tcgtcaaccc aaggaaaata
    45781 cgcctgagca tttagtgttc cgcgaacacg cctttcttta taatctcctt gttggatatc
    45841 ataacttttt gttacaaaaa atgaagcatt ctttttagga acaggttgca tttgtgcatt
    45901 ctgatactgg acagattgga tcgtatggtc agtgtaaatg tattcaacga atggcggttt
    45961 caataaaccg acaccattgt cccaagcaag atgatcaaac ccaagctggt ccctaaaata
    46021 ggattgaaac atttgattgt ttggcttttg ccagttaaag gggtctcgat aaagctggat
    46081 acatatgact tcgttttcta attccttcac tttttctact tgttggatgt cgagaaaaat
    46141 cggtttaggg atgatttgat gataatagcg actataatac atttcccaac acgaagtgaa
    46201 acataaacct tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga ctactacttc
    46261 cccgttttgt tgtttcattt taggtaaatg ttcgatttca tcctttttct caaacaatag
    46321 ccgttgagag atctgttttg tattgtgata gataaactcg ctataagctc gcatataggc
    46381 gaacacccca tgagcaatca tttttttctg aatgaagtct cgaatcaaaa attgcttttc
    46441 tttgaaaaca gagatagaca atacattttt tatcaataat tgattatctt ccaagcgaaa
    46501 actacattcg ttctcgtcat ttcctacaac taaccaactt tcgtctctta ccgatgctag
    46561 cattattttt tcttgaagct ttcgaagatc atacggatcc gcttgttgtg tgaaagagcg
    46621 gataaatgtt ggctggatca acagacgtgt caaaatagcc caatcgctta tttcctgttt
    46681 tttttcttct tcatttaagt ctgtgagctg gatagtcatc aataatttat cttcgccaat
    46741 cattttctca cctctttcaa atctttttat aatttaatta taaccaatga taaagaaaaa
    46801 ctaaaacttt atgtcttaga aaccaatttg gacgattaca tgatagacta agtaaaaaga
    46861 aacaaagagg tgtttacaat ggggttgaaa tttgaacgta cagataattt agataaactt
    46921 ttcgaatcat ttgctgtcga tccggaaaaa acagttgcac caaaagaaga gatcgatcga
    46981 tttctaaaac cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa gtaagttaag
    47041 ctcaaaaaaa gacttaccag ccaaactttt atgaaagagt ttgactggta agtctttttt
    47101 attcttttta tgattcaatt gcttgttcga ttgctgccca cgcttcttct tttccctgtt
    47161 ttgtaacgga agaaaagaga ataaacgtgt cattgcgatc aaaatccaac gcttttttga
    47221 tttcgctctc atgcttgttc catttaccac gagggatctt atctgcctta gtggcaacaa
    47281 caattacagg gatatcataa tacttcaaaa attgatacat ctggatatct tcttgagaag
    47341 gcttatgacg caaatctact aaagaaacga ccgcacgtag ctggtctcta gtcgttaaat
    47401 atgtctcgat catctttccc cattttgctc gctcagtttt ggatactttt gcatacccat
    47461 atcccggaac atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc aatgtctggg
    47521 tctttcctgg cttaccggaa gtacgggcta aattctttcg atcaatcaat gtgttgataa
    47581 aagaagattt ccctacattt gatcgtcctg ccagagctat ttctggcaat tgtgtatctg
    47641 gatattgttt tggtgccact gcacttatga taatttcagc ttgatggact ttcatggtct
    47701 ctctccttaa caaggtgatg aataaagcaa gcggctgtcg caaaagccaa aatgactttt
    47761 acggcagcct cttttcatca acccgctttt ttctctgaat ctttatagat tactgttggc
    47821 tcaccagaca attcagctgc ttcttttgtg ataatcactt tttcgatgtt ttcatttgaa
    47881 gggatatcaa acatcacatc catcatgatt tcttcaatga tggaacgaag tcctcgagca
    47941 ccggtattgc gttcgattgc ttttttcgca atggctctca acgcttcagg ttcgaattca
    48001 agttcggtat tatctagaga aagaagtttt tgatattgtt tgactagcgc atttttcggc
    48061 tcagtcaaga tccgtaccag atcatctgtt gtcaatttct ctaaagctgc cataacaggt
    48121 aatcgtccga taaactcagg gatcaatccg aattttagta aatcttcagg aatgatatgc
    48181 tgcatgacac tttcgtcttc agaaagtttt tggttttgtg taccaaatcc aatcgtcttt
    48241 tcgcccatac gattcttcac aatcgtttcg atgccatcaa atgcaccacc tacgatgaac
    48301 aatatgttcg tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg tccgccctga
    48361 ggtggcacac ttgcaacagt tccttctaga attttcagca aggcttgttg gacaccttcg
    48421 ccggaaacgt cacgagtaat agatacattt tcgcttttac gcgcaatttt atcgatctca
    48481 tcaatataga tgatcccttt ttctgcaagt tccacattgt aatcagcaga ttgaagcaat
    48541 ttaagcaaaa tgttttctac atcttccccc acataaccag cttctgtcaa acttgtcgca
    48601 tctgcgattg caaaaggtac attcaatgtt tttgctaatg tttgtgctaa aaatgtttta
    48661 ccagaccctg taggtccgat caagcagata ttgctttttt gcagttcaac atcatctgtg
    48721 ttttcttgtg cttgctgatt gacacgttta tagtgattgt atacggcaac agctaacgag
    48781 cgttttgcgc gatcttgtcc aatcacgtag ttattaaata cttcaagaat ttctagtggt
    48841 ttagggactt cagtgaattc gcgtacagct tcttcataaa attcttcatc aatgatttct
    48901 ttacataggt cgatacattc attacagatg tagacccctg gaccagctac gatctttttc
    48961 acttcttctt gggttttccc gcaaaaagaa cagcgtacgg tctcattgct acttggattg
    49021 tcatacatgg tcttcacccc ttaacttgca aaaagtatgc aagaaactat caaagacttt
    49081 acagtctttt actatcataa catagtttag taaaaagaaa aagtgtttaa caaaggatca
    49141 actgacctat acctttgcat tttttactct taacaaaaaa taaggaactg tgacaaaatc
    49201 gtgtcacagt cccttatttc gaataaacaa ttttctagaa gtagcttctt cagaaatgat
    49261 ttattacttg atttctattc tttcgcttct gtcgctgtac ctgtgatcaa ttcgattgct
    49321 tttttcattg tcacatcatg ttccagcata tcttctgtca acacgcgttt gatttgttcg
    49381 attggcatgt tgtatgtttc tgataattct tggatttctg cattgatttc ttcttctgtt
    49441 gcttgaagat tttcagcagc tgcaattgct tcgatcacaa gatttgttct tgtgcgcatt
    49501 tctgcttcac cttcgaattg tttatgcaag tcttcttctg tagaaccagt taattgatag
    49561 tacatttcag gagagatacc ttgacgttgc atgttgttca agaattcgtc cattgaacga
    49621 tgtacttcat catgaaccat cacgtgtggc aattcaacga tttctgcgtt ttcaacagcc
    49681 atacggatcg ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt ttctactaat
    49741 tctttgcgat atttttcttt caattcgtct aatgttgata cttcgtcatc aacatctttt
    49801 gcgaattcgt catctaattc aggcacttct tttgctttta cttcatggat tgtcactttg
    49861 aagactgctt cttttccagc aagatcttct gcttgataat cttctgggaa agttacattt
    49921 acatctagac tttcacctga tttatgacca actaattgat cttcaaaacc tggaatgaat
    49981 gagcctgaac ctaattcaag tgagtaattt tcgccttttc cgccatcaaa cgcttcgcca
    50041 tctacaaagc cttcgaagtc gattactact gtatcgccat tttcagctgc agcatcttct
    50101 ttgataacta attcagcttg tgcttcttgt tcacgtttga tacgtgcatc cacgtcagca
    50161 tctgttactt cacggtcttg tttttcaaca actagatctt tatattgtcc taattttact
    50221 tctggtttca ctgttacttc agcagtgatt acccagtctt gacctttttc catgctttca
    50281 acatcgattt ttggttgaga aacaggatcg atgccagctt ctttgacagc agcttcataa
    50341 gcttctggta atacagcatt caatgcatct tcatataaag cttcttcacc gtacatacgg
    50401 ttgaatactt gacgagaaac tttcccttta cggaatccag gtacgttcaa gctttttttc
    50461 actttattaa acgcagttgt taatcctttt tggatttcgt cttgcgcaat cgtaaatttt
    50521 aaaacaccat cattcgtgcc ttttttttca aatgttgcag tcatttaatt ccctccgagt
    50581 ttagaaattc attttataca tcaaaaacgt ttcatgcata cctttaaata gtacactaac
    50641 cctttcaaat tgtaaacata ttgaatggat agaatccttg tttttcataa catttttatc
    50701 tttttcactg ataaaatcta atggaagaag atcatttgct gcaaaatttt ttctcttttt
    50761 tttaaaaaat cttgtttttc atcttctgta ccttcataca tcgtttttcc tgtacacgcc
    50821 tctacagtca gttgaaacca gcgcttcggg tcttcgatgt atgtatcata gacaggataa
    50881 acaattgcag attgcaacgt gaattcttct tttaacacgg aactaagcag cgcatcttca
    50941 ttcccgtaac gatctgaaaa tacacataat tgctggtaac ttgcctgctg ctgcggcttt
    51001 ggcagatctt ttggtatgac tttgactaac ttttcttcaa tcgtcaaata gaaaacagga
    51061 tggcattcgt tcaattgcct aagtgtctcc agtaattttg cccttgctaa aatatggacc
    51121 tctggcgata caataagttt actcgctgtt tggataaact catgataagg aagctgttcg
    51181 atctcatcaa tagacgcaag ctgacgtgca ggttccaacg aaggaagttc ctccgattta
    51241 ttgtggagtg ccttgatctt ctgcctctcc atttgtccgg ataattcttc tgcaaattgg
    51301 attttttcaa tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc tatcaatttt
    51361 ctagcatata aaaaatcttg tgtactgatt aacaagtcaa aataaaattc agcaaactcc
    51421 tcattttgaa gatactgttt ttcatgcaag agtgcttgct tcaatgcttc ttttttctcg
    51481 tccaactcgt aaagacaaaa aacaagcagg ctattagcct caaagctttc tttcaactcg
    51541 taagctcgtt gaaataattc ttttgcctgc tgatattgct ggtttgctag tgcttgttgt
    51601 gccactcgca ttaaattctc atatttttct ggaaaatcaa tcggcttcac gcagtgtcct
    51661 cctcttgctc atattcagct catgttcaat agtagcaaaa caaaaaaagc atgacaatta
    51721 aaaataactg aagcaccctc taaaagctag attttagatc tgacttttag aaggtgcttt
    51781 caactgttac tgctttttat tgctcatcag ctgttgactt cctggttgaa cgctttgatc
    51841 agattgatcg taacaacact tgtattagtg aaaaaatcgc ccagatgttg ctttttctga
    51901 ttaaagactg ctggaagata ccctaacatc agcaaaggaa attttagaat gaaccggcaa
    51961 gcaccttctc taactaaaac ggttgaccag ctaagttctt cttcatcaaa gctgatgact
    52021 cgaataccaa agatcatttt cccaatcgtt tggccatgat tgaatttcgt caataaaata
    52081 aaatacgcta gataaataag taaggctgtg aatccgtaga gactcagata agaatctgaa
    52141 aaattaagcc aacctagatt tttggcaata ccaagtgtta aacgcgtaat agctccgata
    52201 cacaaaagat cgaccaagaa ggcaaacatt cggataaaaa aacctgcata gaaatactta
    52261 ggaaaatcat ttgcttttgg cggttcattt cttgtgtccg tatacttttt ccatttacgt
    52321 tgtttctttt tgatttcttc gggcgtcaaa ggctctcttt caaaagagct cagtaccttt
    52381 tgggaaaagt tggagtcagg tcctacagca ggctgctgtt gcttatcttt ttcttgttcc
    52441 ttattttcat ttgaaagctc tgacatgctt actcacctcc gtaataatac attgcttttg
    52501 gtgattccgc tgtccctaga ttctcaagaa cggataagat ctgactagtc tcagaaggct
    52561 tcaatccctg gaattctgct agtttacttc ctaaccatgt actagcaaac ccagatggcg
    52621 tggaagaata ttctactagt tccgcatctt caagctgttg ttcctttcgt atagctttta
    52681 aagcatcttc aggaaatccc aattcatcga ctaaacctac ttcttttgct tggacaccat
    52741 cataaatcct tccatcagca atttttttga cttcttcttc agatttattc cgtccctcgc
    52801 tgacgattgc tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc accgcttctt
    52861 cttcttttgt ctctggtcgc gttcctgaac ccatatcttt caacgctccg cttttaactg
    52921 ttgtatcttc cacacccagt ttttctagaa gtcctgaata attcaatcct gacatgatca
    52981 caccaattga gccggtcact gtttcttctg ttgcaaagat tttatctgct tgagcagaaa
    53041 tatagtagcc accgctagct gccatgtttt tcatgctgac atacattggg atatcatgtt
    53101 ctttacgaat cgtatccaat ttcttagcga tttccgcact ttcgtagaca ccgccaccag
    53161 gagaatttac ttctaaaaag atacctttga ctgttggatc ttcttcaatc gccttcagtt
    53221 gttccataaa atttttatga ttgtaacctt cagaagcaaa taaacctgaa gaccctcccg
    53281 cagcaatcgt tccatctact gataatttta cgattttttg cgtactgtct ccctcttcca
    53341 acacatttgg agctagttct tcatccccat ataaccaact gttcaatccc tcaatttgat
    53401 cgttttcctg aggcttagtc aaactagctg aaactaacga aaccacaaat aatcctgcag
    53461 caatcagaac tgctgcccaa cgtcttttat tcattctttc tcctttctaa tacaagtttt
    53521 ttatgacgat cacagtttct gactgctcag aagtgtcgaa ccgctctgct ttttcttccc
    53581 gcgcataccc ggaaaacgac tcttttgtta attcatcttc taccgtaaac tcacgagctt
    53641 gagcaacttc caaggcatca ggtgcgattt cttggatgct atcccagcca gtatctaaaa
    53701 atattttcgt atcatctgat acaccagctg atgcttcgaa tttggacaat tgttttttta
    53761 acattcccag tgtcatgatc tcaatttttg aatccatttt tactcctcct ctatccgatt
    53821 actttctatt gtaccgaact cctgaaaaag atgatagata aaaacccatt attttttgac
    53881 ataaaaagga catttgacaa agaatttgat atataccaat ttatttttta ttcttttttt
    53941 gttgacaaga acattcgcaa aaggttatga ttttaaatgt ttccagataa aaaacaaaaa
    54001 ggaggatagt tatacctttt aggatagcgc caggtctgtg taaacagatg acgaggagag
    54061 ggtttatcga aagattcggc ggatggccct agggactgca ctctacagaa aatccacaaa
    54121 aaatgtctgc aaagataaaa caaaaggatt ttcctgcagc tggaagcatt catacgaatt
    54181 cattatagaa agtaggatgt tttttatgtg cggaattgtt ggaatgattg gattaaaaaa
    54241 tgtaacacca ggattgattg acggtctaga aaaattagaa taccgtggtt atgattcagc
    54301 tggtatcttt gtctcagatg gcacaactga ttatctggtc aaagcacaag gcagaattca
    54361 aaatttgaaa aacaaaataa atattgatac tacaggaaat ataggtattg gtcatacacg
    54421 atgggcaact cacgggcaac cttctgaaga aaacgcccat ccacatactt cccaaagtgg
    54481 acgcttcgtg ctcgttcaca acggcgtaat cgaaaacttt gaagaactta aaattgctta
    54541 tttagcaaat gaccacttta ttggtgagac cgatactgag attatcgctc atctaattga
    54601 aactttcgcg aaggatacta caacgcaaga ggcgttttta aaagctttgc gggtcatcaa
    54661 aggttcttac gctttcgctt tgattgatcg gacagcacct gatgttattt acgtcgctaa
    54721 aaataaaagt ccactattaa tcggactcgg cgatggattc aatgtgattg ccagcgatgc
    54781 aatggctatg cttgctcata cgaaggaatt cgttgagatt gaagatgaag agatggttac
    54841 cgtcacatca gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc
    54901 attcgaagca caagtagatg cttcagatat cgagaaaggt acctatcctt tttatatgct
    54961 gaaagaaatc gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga
    55021 agacaatcgt gtctctcttg atcaaaagct ggtcgataca ttgtctgata gcgatcgaat
    55081 ctatatcgtt gcttgcggaa ctagttacca tgcggggtta gcaggtaaac agactttgga
    55141 aaaattgaca cagattcctg ttgaggttca tttagccagt gaatttggct ataacacacc
    55201 tcttctttca aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa cagcagacag
    55261 ccgccaagtg ctggtaaaaa tcaaccgtct cggctaccct tcacttacga ttacaaatgt
    55321 tgctggttct actctttcgc gggaagcatc ctttacactg ctattgcatg ctggacccga
    55381 aatcgcagta gcatcaacga aagcttatac tgcacaaata gctgtactaa ctttattagc
    55441 caaagctatc ggtgataaaa aagaatttga aacttctctt gtattcgata tcactcatga
    55501 attgagttta gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt atttagaaga
    55561 gttggcagaa gtatatctca gcgatacaag aaatgccttt tacattggac gtggtgctga
    55621 ctacaacgtt tctcttgaag ctgctttgaa attgaaagaa atcagttata tccaggcaga
    55681 aggttttgcc gctggcgaat tgaaacatgg aacgattgct ttgatcgaag aaggaacacc
    55741 agtgatcgga atcatcagtg aagaagtgac tggtgcgcat actcgaggaa acctgaaaga
    55801 agtcgaaagc cgtggcgcta aaacactagt tattgtctca gagggattag aaaaggaaag
    55861 tgaccagctg gtgctaccaa ctgtccaccc ttatctaact actctaacca cagtcgtacc
    55921 gactcaatta cttgcatatt acgcaacgct attacgggga ttcgacgttg acaaaccacg
    55981 taatttagcg aaatcagtga cagtagaata ataaaa
//
LOCUS       DASJOX010000006        55677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJOX010000006 DASJOX010000000
VERSION     DASJOX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.006"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            249..569
                     /locus_tag="U4A12_000327"
     CDS             249..569
                     /locus_tag="U4A12_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN1772742.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            723..1628
                     /locus_tag="U4A12_000328"
     CDS             723..1628
                     /locus_tag="U4A12_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1772743.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1625..2398
                     /locus_tag="U4A12_000329"
     CDS             1625..2398
                     /locus_tag="U4A12_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1772744.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2579..4003)
                     /locus_tag="U4A12_000330"
     CDS             complement(2579..4003)
                     /locus_tag="U4A12_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1772745.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4214..4660
                     /gene="nrdI"
                     /locus_tag="U4A12_000331"
     CDS             4214..4660
                     /gene="nrdI"
                     /locus_tag="U4A12_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1772746.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4725..6707)
                     /locus_tag="U4A12_000332"
     CDS             complement(4725..6707)
                     /locus_tag="U4A12_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1772747.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6721..7617)
                     /gene="murQ"
                     /locus_tag="U4A12_000333"
     CDS             complement(6721..7617)
                     /gene="murQ"
                     /locus_tag="U4A12_000333"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1772748.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7620..8681)
                     /locus_tag="U4A12_000334"
     CDS             complement(7620..8681)
                     /locus_tag="U4A12_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1772749.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8892..9458)
                     /locus_tag="U4A12_000335"
     CDS             complement(8892..9458)
                     /locus_tag="U4A12_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1772750.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9486..10988)
                     /gene="eat(A)"
                     /locus_tag="U4A12_000336"
     CDS             complement(9486..10988)
                     /gene="eat(A)"
                     /locus_tag="U4A12_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1772751.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11246..12367)
                     /locus_tag="U4A12_000337"
     CDS             complement(11246..12367)
                     /locus_tag="U4A12_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1772752.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12493..13434
                     /locus_tag="U4A12_000338"
     CDS             12493..13434
                     /locus_tag="U4A12_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1772753.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13584..14480)
                     /locus_tag="U4A12_000339"
     CDS             complement(13584..14480)
                     /locus_tag="U4A12_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1772754.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14749..16089
                     /locus_tag="U4A12_000340"
     CDS             14749..16089
                     /locus_tag="U4A12_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1772755.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16307..16750
                     /locus_tag="U4A12_000341"
     CDS             16307..16750
                     /locus_tag="U4A12_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1772756.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17254..18672)
                     /gene="nhaC"
                     /locus_tag="U4A12_000342"
     CDS             complement(17254..18672)
                     /gene="nhaC"
                     /locus_tag="U4A12_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1772757.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19075..20298)
                     /locus_tag="U4A12_000343"
     CDS             complement(19075..20298)
                     /locus_tag="U4A12_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN1772758.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20329..21168)
                     /locus_tag="U4A12_000344"
     CDS             complement(20329..21168)
                     /locus_tag="U4A12_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1772759.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21185..21865)
                     /locus_tag="U4A12_000345"
     CDS             complement(21185..21865)
                     /locus_tag="U4A12_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772760.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21867..22940)
                     /locus_tag="U4A12_000346"
     CDS             complement(21867..22940)
                     /locus_tag="U4A12_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1772761.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(22996..23234)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23277..23510)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23587..24258)
                     /locus_tag="U4A12_000347"
     CDS             complement(23587..24258)
                     /locus_tag="U4A12_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1772762.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24345..27023)
                     /gene="mgtA"
                     /locus_tag="U4A12_000348"
     CDS             complement(24345..27023)
                     /gene="mgtA"
                     /locus_tag="U4A12_000348"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN1772763.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27682..28119
                     /gene="ndk"
                     /locus_tag="U4A12_000349"
     CDS             27682..28119
                     /gene="ndk"
                     /locus_tag="U4A12_000349"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN1772764.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28141..29007)
                     /locus_tag="U4A12_000350"
     CDS             complement(28141..29007)
                     /locus_tag="U4A12_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1772765.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29167..31260)
                     /locus_tag="U4A12_000351"
     CDS             complement(29167..31260)
                     /locus_tag="U4A12_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1772766.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32009..32632)
                     /locus_tag="U4A12_000352"
     CDS             complement(32009..32632)
                     /locus_tag="U4A12_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772767.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32791..34188)
                     /locus_tag="U4A12_000353"
     CDS             complement(32791..34188)
                     /locus_tag="U4A12_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1772768.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34220..35191)
                     /locus_tag="U4A12_000354"
     CDS             complement(34220..35191)
                     /locus_tag="U4A12_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1772769.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35565..35990
                     /locus_tag="U4A12_000355"
     CDS             35565..35990
                     /locus_tag="U4A12_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772770.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36478..37179)
                     /locus_tag="U4A12_000356"
     CDS             complement(36478..37179)
                     /locus_tag="U4A12_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1772771.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37195..37635)
                     /locus_tag="U4A12_000357"
     CDS             complement(37195..37635)
                     /locus_tag="U4A12_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1772772.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37635..38210)
                     /locus_tag="U4A12_000358"
     CDS             complement(37635..38210)
                     /locus_tag="U4A12_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN1772773.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39058..39537)
                     /locus_tag="U4A12_000359"
                     /pseudo
     CDS             complement(39058..39537)
                     /locus_tag="U4A12_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39696..40397)
                     /locus_tag="U4A12_000360"
     CDS             complement(39696..40397)
                     /locus_tag="U4A12_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1772774.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40505..41914)
                     /locus_tag="U4A12_000361"
     CDS             complement(40505..41914)
                     /locus_tag="U4A12_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN1772775.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41962..43827)
                     /locus_tag="U4A12_000362"
     CDS             complement(41962..43827)
                     /locus_tag="U4A12_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1772776.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43948..44799)
                     /locus_tag="U4A12_000363"
     CDS             complement(43948..44799)
                     /locus_tag="U4A12_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1772777.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45109..45822)
                     /locus_tag="U4A12_000364"
     CDS             complement(45109..45822)
                     /locus_tag="U4A12_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1772778.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45890..46216)
                     /locus_tag="U4A12_000365"
     CDS             complement(45890..46216)
                     /locus_tag="U4A12_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1772779.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46339..46566)
                     /locus_tag="U4A12_000366"
     CDS             complement(46339..46566)
                     /locus_tag="U4A12_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1772780.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46888..48141)
                     /locus_tag="U4A12_000367"
     CDS             complement(46888..48141)
                     /locus_tag="U4A12_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1772781.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48202..48942
                     /locus_tag="U4A12_000368"
     CDS             48202..48942
                     /locus_tag="U4A12_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1772782.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49028..49786)
                     /locus_tag="U4A12_000369"
     CDS             complement(49028..49786)
                     /locus_tag="U4A12_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1772783.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49783..50811)
                     /locus_tag="U4A12_000370"
                     /pseudo
     CDS             complement(49783..50811)
                     /locus_tag="U4A12_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50831..53998)
                     /locus_tag="U4A12_000371"
     CDS             complement(50831..53998)
                     /locus_tag="U4A12_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772784.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54222..55106)
                     /locus_tag="U4A12_000372"
     CDS             complement(54222..55106)
                     /locus_tag="U4A12_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1772785.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55201..55638)
                     /locus_tag="U4A12_000373"
     CDS             complement(55201..55638)
                     /locus_tag="U4A12_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772786.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        15989 a        11731 c         9593 g        18364 t
ORIGIN      
        1 aacaacttct ttctatgaaa ttgcaaagca ttcatcttct tttccgctaa cgatatatcc
       61 gtcagtgctt ggtacacttt actaaaaagt atgagattgc cgatatctgg ataggtgtgc
      121 tatactacag aaaaagatat acaatctgcc accgggtagg ataaagagga tgatcattcc
      181 attaggtctt tgaagggatg attttcctct ttttgtgtat ttttttatag ttaaggagga
      241 atgtaagtat ggcatggatg gaattaatca tagcaggaat tttggaagta ttctggtcaa
      301 cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac acagtgattg
      361 gcatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgccg atgagtctct
      421 cttatcctat ctggacaggg attggcgcag ttggttcggt cataattggt gtggtcttgt
      481 ttcatgataa gctgaatctt tccacttggt tctttgtggg gatgctactc atcagtatca
      541 ttggcatcaa gattactagc ggccactaga cacttggaat aagtactgat cagaaaaaat
      601 aagccttttt atttaatatt caattttttt atgaaaaaat aagaaataag tccaatgacc
      661 tagcgacttc tccactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat
      721 ttttgaaaag tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca
      781 gcgatgtcag ttttagctta tcccctggca aaatcgttgg attagtaggt cctaatggcg
      841 ctggaaaaac aacgatcatg aaagctattt taggactgat ccattactca gatggaacaa
      901 tctgtattgg tgaaaatgag atttctccct ctagtcataa agggttggaa caagtaggag
      961 cattgatcga atacccgggt atctatccat tcttaacagg ctatgatcat ctgaagttat
     1021 tctctgaaac caacgatgta tctgctatcg acactatcgt gaatcagctc aagatggaaa
     1081 agtacattca caaaaaagcg aaatcctact cgttgggaat gaaacaaaaa ttgggaattg
     1141 ctcttgcctt actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg
     1201 atccacaagc aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct
     1261 ctttcttgat ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc
     1321 tgatcaatca aggaaagatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc
     1381 aagatttcat cgttatctct acaacaaatg atctagctgc tcgaacgatc ttgacagaag
     1441 ctggatatac tttggaagat cctgataaaa tcaagctttt aaaagaaaac gaagaattat
     1501 taggaaccgt gatccagcta ttgtctgcac aaaatatcca tgtgacagac gtccagcatc
     1561 tgacgaatga tttggaatct tctctattgg aaattctgga ttcagtaaag ggggaagaag
     1621 aaaaatgaca ttgcttgtaa aacaagaatt atttaaattg atcaagaaaa agtcaactgc
     1681 tgttttatct gttctattag ttgttttgct cataggaaca gcacttctag ccaaaaaata
     1741 cacgacgatc attgatccag ttgaaatgac tgctcagctt tttagtgcca catcttggat
     1801 tgtctttatc atgattgcag ctgcaagcac gatcatttcg atggaagcac aatatggtac
     1861 attgaaaaat ctcctttatc gaaagtattc tcgaggagaa atattagtaa gcaaatggat
     1921 cacattagtt atttattctg tttatttata ccttttagct attattgtca ctgtgttgat
     1981 gaaactgatt cttttccctt cgatttcctt cacggagaag gtttctactg gtcaaacact
     2041 gatccaaagc ttgttcactt atactttggg tagttatatc ggtttatggc tgattttaag
     2101 tttagtgttg atgattgctt gctttatcaa tagttccggc gcatccatct cagcaggaat
     2161 cgtcttttat tttgcttcgt caatcatatc tggtatcctt attgcactca tccaaaaatg
     2221 ggagtggatc aaatggaatc cgatcagtat gttgaatctg caaaaccaaa ttggaaacga
     2281 agagatcatg aagcaattga ctcatctttc tactaatcaa atgctattcg gtaatttagc
     2341 ttacatcgtt ttgttcttag cattaggata tctcgtattt aagcgaaaaa atgtatgatg
     2401 actcatcaaa aaagaggctg tgaaagagac gttcagcctt attgccgatt ggctgtctct
     2461 taaacaccgt ttattctgaa aaaggagctg tgacaacatt tgtgtcacag ctcctttcct
     2521 agtatgtttt tgaataaacg acaggctcac aacctctctt tatacttctt ttatacttct
     2581 atacgtgaag catcccagtg gcaaataaaa ccattgcaat cactccgacc atcgtcacac
     2641 ctataagtaa aatcttatct aagtggatat gttgtacagg ctcgttttgc tccttacgtg
     2701 ctttgaggta aaaataaatg cctggcaaga aaagaacaag aacaagcatt acttcattaa
     2761 aggctacgac tagcatagcc catacttgaa aggcacttgc taaaagccct actaaaaatt
     2821 ggacccattc tttccgctga taagaaagtt tcatttggta caatcctaag aaaagccacg
     2881 tgaagaaaat agctgctgta ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata
     2941 ataatgaaag taaaaagaca ttcgtacata acgtcgtcaa tacgatagca taagttggtg
     3001 ccccattttt gttgagtttt ccccatgctt taggcaaaag tccgtcttca gccatgtttc
     3061 ccattgtttc aatcggtaag atcgtccatg agagccaaac accaactaaa gaaatgatca
     3121 aaccgccgtt gatcacgact gctccccatt taccaaccat catttcaaac acatagccca
     3181 tagcaggctg cgccttgatc gtttgcagct gattcatcgt cattgtccca tatggaagca
     3241 tggaaaccaa gacgtaaata ataatcaagc cgaatatccc tagaatcgtt gctttcccaa
     3301 catctgattg tttttttgct cgtgaaccta cgacagaggc tccttcaata ccaacaaata
     3361 cccaaagcat caccatgatg caactcaata cttgactgga aaacgggatc ggctgtccat
     3421 tcgcattcaa tataatgtcg ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc
     3481 tgatagctac tacaataaat aaaaataatg gcactaattt acagaatgtg acgatagcat
     3541 tgacgaaact agcattttcg actccccgat tcactagcca tgcatatccc cataaaaaga
     3601 ctgtactcat cacgatcgat gccagatttt gcccaccttc aaatactggg aagaaatatc
     3661 ctaatgcgct catcaacaat gttgcaaaag ctacgttgcc taaccagcat gtgagccagt
     3721 aaccccaacc gctgataaaa ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga
     3781 tcccgccttt taaatctgga cgttttttat tgatattatt gatcgacaag gagagcatca
     3841 acattgctgt tcctacgagt acccaactga aaatagctgg gccgccagcc gatccggaga
     3901 tgtccgtcat aataccaaat acgccacctc caatcgctga accaattact aaggcagcaa
     3961 gtggaattaa accaattcct tttttctgtt gctctgtatc catgtaagca tcctccaaaa
     4021 taaaaccgct tttatcaaaa agtataacaa acctttttta tttttactta attaataaaa
     4081 actttaatag aaatataatt ctaccggctt ttttctcatt atacaagcaa agcgcctagt
     4141 ccctttccaa attacaccac caaaaaactt aaaaaagatg ctatactaaa aggaaagaat
     4201 tggaggatga tccatgaaca ttttatatat atcgatttca ggaaatacgc gtgcctttgc
     4261 caaacatcta gcagaatatg ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt
     4321 tactttaaaa gaaatccatg aaaatagtga ttttacgaaa gaaacagaac cattttttac
     4381 tttcgttcct acttatttag acggcggaaa tggtttagat aacggtgata cagaaatctt
     4441 gaccgaaacg atgcgtgaat atctggaaca tgaagacaat cataaacttt gtcttggtgt
     4501 agtcggtagc ggaaataaaa acttcaataa tcagtattgt ctaacagcca aacaatatgc
     4561 acaaacattt ggctttcctt tcttggcaga ttatgaatta cggggaacac catctgatgt
     4621 gacacgaatt tatcagattt tagcagaaaa caataaataa taaaaaagaa gaagctgaaa
     4681 aatagatttc agtttcttct ttcgtttcta ttgtttctat catattaagc tttgacatcc
     4741 atcacaggtg cttttgcttt tgctttacct gttttttcca agacaaagtt ttttacacga
     4801 tccatattgg ttaccaagac gatcatggat gtttctttgc ctgctgcttt gatcgcttct
     4861 aagtcagctt ctgctacaag cgtatcaggt gtcacttgct gcccttctgt tactttcaca
     4921 tcaaatggcg taccgcctaa gtcaactgtg ttgattccca tatgaagcaa gatttctaaa
     4981 ccatttggcg ttgtgatccc aatagcgtgt ttcgttggga agacagagct gactacccca
     5041 gtaactggag aatagatatg tccgttttct ggttctacaa agtagccttc acccatcatc
     5101 ttttgtgcaa aaactggatc acttgcttta tctaaagaaa caactgttcc gtcagcaact
     5161 gatgagagga tagtcacact ttcagctgaa gtttgaggta ctactttttc aggcacttca
     5221 tttactgtgg ctgtttcagt cgctaattct tctttttcta aacggtcttt aggaataccg
     5281 aagaagaatg tagcaacgaa gccaccagca taagctgcca ataatccaag gatgtagcct
     5341 aaccatttac cgtcagagat caatgggatc aaagcggctc ctgaaggtcc aatagcgata
     5401 gccccgacat ttccgatcat tccgatcact gccccgccga tacctccacc gatacaagca
     5461 gtaataaatg ggcgtcctag aggtaatgtt actccgtaaa tcaatggttc gccgattcct
     5521 aggatcccga ctggtaaagc tcctttgatc atttctgtta attctttatc ttttttgcag
     5581 cgaatccata atgccaatgc tgcacccact tgtcctgcac cagccatcgc aagaattgga
     5641 agcaatagcg tacgaccagt ttcatcaatc atttgcacat ggattggagt caaaatttga
     5701 tgcagtccga acataaccat cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct
     5761 ccaccaacgc caagtaccca gttgatccca ccgaccatgc catcagaaat gaaacctgct
     5821 aaaggcatga tgaagaaaat ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta
     5881 acgatgatat ctaccgcatc aggtactact ttatgcagtt ttttctcaac gattgacaat
     5941 agccaaacag cgaagatgac acctaggatc ccaccttgtc cagctgcaag agcttcacca
     6001 gtaaataagt ttttcagtgg tgcttccgga ttcatccctg tcagcatcac aacggccccg
     6061 ataactccac caagagttgg tgttgcacca aaaacttgtg cagcattgat acctgtatag
     6121 ataactaggt agctaaacat cccgtttttc aagatgttca tcacatcgat atattgttgc
     6181 catgttgctg catcaagtgt tccggctgtg actaagtttg ctaaaactgc ggcaatccct
     6241 gccacgatcc cagtaccgac aaatgcagga atcattggca caaagatatt agaaatgtct
     6301 tttaataccg atttgatttt agatggtttt tgtttcgatt tttgtgccgc tttcatttct
     6361 tgtgcccgtt cgtttacacg gtcttttcca gatgctgatg aatttgctgt agctgtactt
     6421 acagaagaaa gtttttctcc taatttcacg cctgccaaat caaccatttg ctgtgctact
     6481 ttgttcactt tcccaggtcc gatgatgact tgaagctgtt catcttctac tacaccaagc
     6541 actcctggaa tcgcttttaa ttcagagaca tttacaagat catcattgat gatcttcata
     6601 cgcacacgag tcatgcagtg gacaatgctt tctacattgc ccatgccgcc tgccccttta
     6661 taaatttgtt ttgccagttc ttccaaagtc agttcgtttt ttgccatggt cttttccccc
     6721 tcataaggtc ccttttacaa atccgtttgc ttcagctaat tttgcttgag cttcctcttt
     6781 atcactattt gtcaaaatca tgacgatagc cagcttaaca ttttgatctg cttcttcaaa
     6841 gtacttttca gctgtttcgt aatcagcttc tgtcgcttgc atgatgattc gttttgagcg
     6901 ttcgattagt ttttcatttg ttggtttgac atcgaccatc aagttattga atactttacc
     6961 tataccaatc atactgatag tagaaatcat attcaagata agtttttgtg ctgttcctga
     7021 cttcaatcga gtagagcctg tcaaaaattc tggacctgca tcgacttcga ttgggaaatc
     7081 cgcgtgttta ctgatttcgg cttgtttatt gcaagaaatc gttcctgtga cagcaccgat
     7141 cgattttgcg taatctaatc caccgatcac atatggtgta cgaccgctag ctgcgatacc
     7201 aataactata tcattttttg tcaaattcag cttgattaga tcttcttgcc ctagctcata
     7261 agaatcttcg gccccttcga cagctacagt cattgcttct tctccaccag caattagacc
     7321 tatgaccatt tccggttcta caccgaatgt cggtacgcat tcagcagcat ctaacacacc
     7381 tagtcttcca cttgttccgg ctcccatata aatcagacgt ccaccttttt taaaggactc
     7441 aatcgtgtta gcaatcactg gttcgatact tgtcagttct ttttcaaccg cagcggccac
     7501 tttctgatct tctttgttca ttagttgaac ggcttgagaa atactcatct catctaatcc
     7561 aaaagtttct tggtttctgc gttccgttgt taggttctct aaattgattt tgctcatttt
     7621 tatcttcctt cttttcttat aaattgatag ttcataccag ctttgatttg gttgatcaat
     7681 ggtagctctt ctttgataac tttagctact acattcactt tttcatccgc tggcaaatca
     7741 tatttggtta attggatctc acccatatag cgcaaatact tttcgttatc taaagtaata
     7801 gaccctttca tacgcgtagc tgtatttctt gctgggatag gcggaatctc tcgaaaacgt
     7861 gcatctacgc ttcgtatgac atctcttgca tcgtcttgcc gattcgtatg cttccctaat
     7921 atatattcgt aaaattcttc atctacaggt tccgtatgga gcaagactgt ctgttctttt
     7981 tggaaagaag caaattgctc ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca
     8041 atatccgtgt tacttgctaa tagatctaga gcgcctgcaa gaggatggat ccctcgatgt
     8101 tcttccaaag ttggcaatcc ttgatatagt gggcctctca actctgtatc tcctggtaca
     8161 aaaccttgga caaggagacc ttgaattttg agccattgat tttttttctg ataccattct
     8221 ttgtccagcc ctgtttcggg acgtggataa taattatgcc aagcttcaat ctgtgagaaa
     8281 tcagcttctg cttctttcaa ttcatcgata tctttaggcg tgatcgtgct agcattcaaa
     8341 ctgattttca attgatgtga ccagtcggca atctgctgat tggaaatatg ataatccatt
     8401 cttaaacctg ttacaccaat tgctttcaaa ggcgttagat cttctaatga aaaacccgct
     8461 cgttccaatg cctctcctga aatatcgacc atcaattcta gtttattctt tttcgcttcg
     8521 cttcctagtt cgatcaatcg cttttgatat gccgaaacat catcttctgg tatatgcatg
     8581 gaagtgaaaa taccgacaaa gccattggat gccatttggc ggatatactg tttcttctct
     8641 tctgacaaag aatcgttcat aaatattgaa aagccaaaca tattatccct ctttttctgt
     8701 atccgtcttc atttctatga ctagagtata tatctaaaga aacaaaattt caatactttt
     8761 attaaaataa ttaaatttca tttctaaagg ttgagtttat attatcaaaa gaattgtcat
     8821 aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat ttaaataaca
     8881 caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa tgtaaagaac
     8941 ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc attatggcaa
     9001 actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg aacgatccgt
     9061 gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc taaacgtgct
     9121 ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc catttctacc
     9181 aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg tgctgtatct
     9241 cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa atctactacc
     9301 ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac atctgcccgt
     9361 ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac ataaagcatg
     9421 tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc ttgagattac
     9481 aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag gaaggtttga
     9541 tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc taattgttct
     9601 tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc agctggctgt
     9661 gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg ctcgatcttg
     9721 ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa tgcttggtag
     9781 tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc ataattttgg
     9841 cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac cttgccgttg
     9901 aatgccccca gaagataatg gataatggat gactttcctg caccgttagg acctgaaatc
     9961 gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag attttcatac
    10021 cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg gctattcatc
    10081 gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct gatctgggtc
    10141 tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc agcacccact
    10201 gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt ctttgtttta
    10261 tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt tagcctactg
    10321 acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag ttttttctgt
    10381 tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc gattgctaaa
    10441 acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat gaagccttgt
    10501 tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa atgattcgtt
    10561 ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag taggactttt
    10621 gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg atctgtttgc
    10681 atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc atttaacacg
    10741 taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa ttcttgctga
    10801 tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa agttgtcttt
    10861 cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt tagatttgct
    10921 tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt tatttcgatt
    10981 ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag
    11041 aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc aagccaatcc
    11101 cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc agctttcatc
    11161 aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg aaattacgat
    11221 agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg
    11281 tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct tcttctaaac
    11341 ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt ccttctagct
    11401 cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt gcaagcactg
    11461 ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct tctcctgtaa
    11521 tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg actaaatcga
    11581 aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg cctgctgctc
    11641 cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc tgagcaatct
    11701 ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt tgtcttccaa
    11761 attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca gcgataatca
    11821 attttacttt agaaacaaga acgtttcttc gatcaaaatg aagtttttta gtagataata
    11881 aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt aatcctagac
    11941 caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact tcttgaagca
    12001 gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata gtttgatccg
    12061 ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt
    12121 ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag agatccatcc
    12181 catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca gtattttctc
    12241 cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag ccatttagaa
    12301 ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa
    12361 tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat ttttttcaaa
    12421 caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca ttgaataaag
    12481 gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag ccgaatcgga
    12541 ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt
    12601 gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa
    12661 ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga cttgatcgta
    12721 gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc cattattact
    12781 gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt actggaaaaa
    12841 tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg tctagctggt
    12901 cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt ggaaccagaa
    12961 ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt aaatccgatc
    13021 tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat
    13081 ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag
    13141 agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga aggaatcata
    13201 ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga caacatcggg
    13261 ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac
    13321 tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac accatattgt
    13381 gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct
    13441 ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc aataaaagat
    13501 caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt ttcagtacat
    13561 aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc atattcgtat
    13621 tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc tgcatcataa
    13681 aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc cggtaattca
    13741 tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg ataatgagca
    13801 atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa
    13861 gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt
    13921 ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg tggaagaatg
    13981 aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc tgctcgttct
    14041 tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa
    14101 ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt tgcttccaaa
    14161 aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc
    14221 tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt ggtaaatggg
    14281 cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat cagctgataa
    14341 tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc ttttccggac
    14401 caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt tgcaactagc
    14461 agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt tcctatgata
    14521 gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga aaatttaaca
    14581 tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct aaaagttttc
    14641 tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat attgctattt
    14701 attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa
    14761 aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc
    14821 ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg
    14881 attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc
    14941 gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt
    15001 gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc
    15061 gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg
    15121 gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta
    15181 ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat cttactgatc
    15241 atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt
    15301 cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct
    15361 ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa
    15421 gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt
    15481 tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca
    15541 ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg
    15601 gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat
    15661 gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta
    15721 gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt
    15781 tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt
    15841 tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg
    15901 gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta
    15961 ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc
    16021 ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg
    16081 aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg agctattgaa
    16141 gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga
    16201 cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct
    16261 catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg
    16321 gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa
    16381 tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat
    16441 gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa
    16501 gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt
    16561 acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc
    16621 tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc
    16681 ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga
    16741 aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag ttatcagctt
    16801 caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg aaaaagctgc
    16861 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt
    16921 aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtaaaatcag
    16981 cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat tttgccattt
    17041 tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt tgtgaaaaag
    17101 tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat ttttgtataa
    17161 atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa agggattgtg
    17221 acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg ctgtcttgct
    17281 tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga aggcactgac
    17341 catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg
    17401 aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta agtagttgat
    17461 tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc ctgctttttc
    17521 ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt caccaataaa
    17581 aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat
    17641 ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca acaatccacc
    17701 taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga ttcctccgcg
    17761 gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg attcgatgac
    17821 cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc ctactgctac
    17881 agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac acagtaacat
    17941 taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta gaacatcagt
    18001 cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa
    18061 aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga tgtgtttgaa
    18121 caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg gagacatctt
    18181 atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat ttaagcctag
    18241 tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc
    18301 tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc actggacact
    18361 gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta tccatacagc
    18421 aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt tgatgccatt
    18481 gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat acaacatgac
    18541 cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga caacgccaat
    18601 cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac ttggtgttcc
    18661 tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga
    18721 tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat aaaaagacct
    18781 gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt
    18841 tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg
    18901 atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa
    18961 gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt
    19021 tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt tttactagaa
    19081 agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc
    19141 taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat
    19201 cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc
    19261 ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac
    19321 atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg
    19381 aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac
    19441 atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc
    19501 acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc
    19561 acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc
    19621 tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc
    19681 attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc
    19741 atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt
    19801 tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc
    19861 ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc
    19921 atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa
    19981 tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc
    20041 gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa
    20101 ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt
    20161 aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc
    20221 tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt
    20281 tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc
    20341 gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt
    20401 gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt
    20461 tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat
    20521 ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat
    20581 gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt
    20641 ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt
    20701 gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg
    20761 gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat
    20821 tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt
    20881 gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa
    20941 agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt
    21001 tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt
    21061 tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca
    21121 aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc
    21181 actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta
    21241 agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc
    21301 tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc
    21361 cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca
    21421 aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca
    21481 ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc
    21541 caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag
    21601 tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca
    21661 gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta
    21721 ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca
    21781 tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca
    21841 aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta
    21901 tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat
    21961 gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt
    22021 tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac
    22081 agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat
    22141 ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca
    22201 agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc
    22261 tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag
    22321 atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga
    22381 ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt
    22441 gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt
    22501 tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca
    22561 agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt
    22621 aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag
    22681 atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc
    22741 gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact
    22801 aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc
    22861 gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa
    22921 acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa
    22981 tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt
    23041 gttcacacac aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg
    23101 tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt
    23161 ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat
    23221 ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa
    23281 aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc
    23341 tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat
    23401 cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca
    23461 gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg
    23521 ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attatttttt
    23581 ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa gatcttttcc
    23641 ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga caaaactgga
    23701 atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga
    23761 atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag ctgtcagata
    23821 aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg tgtccaagta
    23881 atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc
    23941 aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg catatggttt
    24001 taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct tcgctcggac
    24061 ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac tttgtttcca
    24121 gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa attgctgaaa
    24181 tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac catctaaatc
    24241 aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca tagtttagca
    24301 cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa gagttcttgg
    24361 aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc gatgactgta
    24421 gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc agcaccaaaa
    24481 cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac aaacatggca
    24541 aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg gagcactaag
    24601 gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat gaaaatcact
    24661 cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca aataacaaaa
    24721 tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg
    24781 aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc tttatccatc
    24841 cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa caaaagttgt
    24901 aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga aaacatattg
    24961 ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa agtttctcgt
    25021 ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca
    25081 gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc cactttcatt
    25141 gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt tttacgtaaa
    25201 gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc atggtcttca
    25261 gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt aaccaattca
    25321 ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt gtccccagtc
    25381 agaactttta cagctacacc atgttctttc agagctttca acgcttgttt cgttgtttct
    25441 tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc atctttgact
    25501 gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac tcgtaagcca
    25561 tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt tgttaatggt
    25621 gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc aatcgcgcct
    25681 tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat acgacgacgt
    25741 tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt atctaaagtt
    25801 tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag
    25861 ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga
    25921 tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca taacacatcg
    25981 attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt tttggccatc
    26041 acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggtgttaga
    26101 ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt tgtaagccca
    26161 ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag catggatgtt
    26221 ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt ttctgctaca
    26281 tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact tccactgatg
    26341 acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg atcgtcttca
    26401 catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc ttgggaaatg
    26461 aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc tgctgaaagc
    26521 cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt tccattacgt
    26581 tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg attggaacgt
    26641 acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat aatggctaca
    26701 ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa tgaaataatt
    26761 cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg
    26821 aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct tgaacgttcg
    26881 atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa ttcttgtttc
    26941 gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc catctcaatc
    27001 tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc tattcatcgg
    27061 atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc tggcctttcc
    27121 tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta
    27181 attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt agaccacctc
    27241 ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc caaatactca
    27301 gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt tgagttttga
    27361 cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa gccttatctc
    27421 gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg
    27481 tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata acaactaaaa
    27541 aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata cacacgttat
    27601 attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata atagaaatat
    27661 agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc agacggcgtg
    27721 agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact ggccattgct
    27781 gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca gcacttgact
    27841 gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt cgtttatctt
    27901 gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc aacaaaagct
    27961 gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac tgaaaacatc
    28021 attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca
    28081 ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta aactgctggt
    28141 ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc gttgattctt
    28201 tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc
    28261 taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat agatcactgt
    28321 acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac gtgccttttt
    28381 caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa agagatactg
    28441 ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca aataattgtt
    28501 ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata gttcaaattg
    28561 accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa
    28621 ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca aaaccaaata
    28681 atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt tataaggatc
    28741 aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact ttattggttc
    28801 atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc gctctaaaaa
    28861 tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc cgactagaga
    28921 agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt tgactcgaag
    28981 tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa atttttccac
    29041 attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt
    29101 ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga aggttggttt
    29161 gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg gatataagtt
    29221 ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt
    29281 ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg gatcaatcgt
    29341 ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag
    29401 acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga ttcctgctgc
    29461 tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta
    29521 tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct gagaagataa
    29581 taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt
    29641 cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag tataaccagg
    29701 catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc
    29761 tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga tcccaaaatg
    29821 atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa
    29881 aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat ctactgtgct
    29941 attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg
    30001 gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc
    30061 agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt cggattcatc
    30121 aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat gatctctatt
    30181 ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct tttgtccaag
    30241 gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata atgagccttg
    30301 aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct ttccctgtcc
    30361 tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa tactatttaa
    30421 aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct ggaaactttc
    30481 taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg gatttggctc
    30541 tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc
    30601 gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg catcgattcc
    30661 tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc
    30721 ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc
    30781 catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag
    30841 tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct tcatcaaaga
    30901 aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag atttgactgt
    30961 aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa
    31021 aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat acaaagaaag
    31081 gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt actggatcgt
    31141 accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa
    31201 gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac
    31261 gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc
    31321 ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc cgtccacttt
    31381 tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata aaagtcttgt
    31441 ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt acaaaaaaag
    31501 aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca gctttttcac
    31561 aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca caatatttat
    31621 aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa acggcaggct
    31681 caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat tatgtcagga
    31741 cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    31801 ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg gctcagagtt
    31861 aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgacct tcggtattag
    31921 ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa tactcaggtc
    31981 aaaacgctga tttcacaacc tctgtttttc attgtccata ggattgatat tgtccttgtg
    32041 atgactcatt cgtaataatt gaggcttgat tttcttcact tcccgttatc gtttgtccac
    32101 tgccagaatc tgaaggactt gttcctgtcg actcgttttt ttgagaagag acagttgttt
    32161 tcttgacttg ttcatttgtc tttttccatt ccgctaaacg atcggcgacc acttttttgt
    32221 ctactttttc agaataagcg gtgatcactt ttccattttt caaacgggct actttgtcgc
    32281 catcatgagt caaaagcaaa acggtcaaat gcttgtcttt aaattgatat tgtctgagag
    32341 aaatcgcact aagtacgtca tcgatattaa taaattgatt ttcttctttt ccttttggca
    32401 aataaatgtc caattcgcct ttttcgtaat tggcagttat tttatactcc gcccaccaat
    32461 aagttgagaa ttcttttttg aaattatcta ctgattcttt gcttgctacg attttttctg
    32521 tttctttctg agcaagatct gctgtcattt ttcctgaaaa tgctgcggat aaaataagca
    32581 gtaagaaaaa accgaataac caaaagagaa gaaacggtct ttttttcctc atgctttcct
    32641 ccactcctat ttaattttga ctattctatt atatacaaaa aatgaaatag aaagacaaaa
    32701 aagttgaaaa agatcgtaaa taattgaact tctttttcac aaaatggtaa gaatggagaa
    32761 tttcacgcat ttgctctcaa ctattctttc ttaagcctca aatccgtttt tatctgatac
    32821 gtctttgaac caatgacccg attttttgat tgttcttttt ccatcttgtg ctaagtctac
    32881 tgaaataaag ccatatcgat tcttatatgc attggtccac gaccagttat ccatacatgt
    32941 ccacatatga tagcctaaac aattggcacc ttcttgaatg gcttgatgga catatttcag
    33001 atgagattgg acaaattcga tccgataatc atcgtggatc atgccatctt ctttgataaa
    33061 tcgtgcttcg ttttctacac ccataccgtt ttcagaaata aagcagtcga tatttccata
    33121 atgatctcgt acattgatca aaatatcata gattccttgt tcatagatct cccatccgcg
    33181 ataaggattc attttacgac caggcatttc ataattgtcg aaataatcct caggcattgg
    33241 accgttttta gtatcgattg gtgtttcttt tgcttttacc cggcgtggct ggtaataatt
    33301 gatacctaaa agatctactg tattttcttt gataaccgtc aaatcctctg catggatcac
    33361 aggcatgaga tcaagttctt ttactatctc aatcaattca cttggaaact cgccttttac
    33421 agctggatca aggaatgaac gattgaagaa cgcatctgct atttgcgctg ctttaacatc
    33481 ttcagggttt gattcgtctc ttggatagct tggtgtcaga ttcaagatga taccaataac
    33541 accaccttgc tccatttcat ggtaagattt gatggctaac gctgaagcca acgcttcatg
    33601 atagccaact tggatcgctt ttttcatatc tgactcttct ggataatgcc atcgatataa
    33661 ataacctgct tcgactggaa cgattggttc attatgagta aaccattttt ttactttcga
    33721 accaaacaat tcaaaacaac ttttggcata tttttcatag gcttcaaccg tttctcggtt
    33781 caaccatccg cctttttctt gcagatacat cggcatatcg aaatgataca gattcacaaa
    33841 gggttcgata ccgttttcaa tcaactcatc aaaataacga tgatagaaac gaacggcttc
    33901 ttcgttgatt gttccagatt catcaggtat caaacggctc cattggatag atgttcgaaa
    33961 tgaattatgc ccagtctgtt tcatcaactg tacatcctct tgatacttcg tataaacttg
    34021 cgaggttttt tcaggtccta cttgatcaaa gaacttttgc ggttcttttt tgtaccaata
    34081 atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt ccttctgttt gtgggccact
    34141 tgctgcagaa ccccaccaaa aatttgctgg aaattgatat tttttcatca aagcacctct
    34201 ttttcttttt tagttcgttt tagttgattc catgtatgat attttccatc acaatcactt
    34261 tttcattttc ttcctcttct tttttgattc gttttaacag ctgttctgct gctcgttccc
    34321 cgattttatc tgttggtggc gtgatcgtga ttttcggaag gatactcaga tcgtttttat
    34381 ctaaataatc atagtgactg aacgcaatat ccttcccgat tctgacacct tcttgattga
    34441 aacattcgat ggcaccaatc gacatattgt aattgctgac aaaaatagca gttgcccctt
    34501 tttctaataa tgcttttgcc ccgtaatagc cgctttttct agaatagtct cctgtaaaga
    34561 cctctaattg gatcgatgga tcattttgga ccgcttcttt ggctcctgct aaacgttctc
    34621 ttgctacgta gtctgtttga gtggcagcaa taaatccgat ttttttatgt ccttgttcac
    34681 catgatgagc gatcactttt gctacacttt ctcgatcatt cactaatatt gtatcaatac
    34741 ctgctggtcc ttctggacaa ttcaatgcga taactggaat actcattttt gtgagcattt
    34801 caaatccaga ccactcttct tcggataaga agacaatcag cccatcgatc acatgcttca
    34861 tcaggtattc aattttttgt tcaatttttt ctggctgatc ggcaaacccg cttaataata
    34921 agctataccc attttcttca gctactcttt cgatccctga gacaacttcc gacccaaaac
    34981 ggctggaaat cccgtttatc aaaagtccaa tagagaagct tcgattattc ttcaaccctt
    35041 tagcaatgat gttttcttta tagtccaacg ctgaaatcgc ttctgctatt tttttcatgt
    35101 tcttttcctt cagttgctga ccattcaagt aacgggaaac tgttcccact gaaacaccag
    35161 ccatcttagc cacatcacgg atcgatgcca tacattctca ctcctgccag ttacatttga
    35221 tttctctttc ttgttgttcg attatgatga ccgcattctt tgcatagtgt tgcttgtcat
    35281 caatatccat ataactaaag tcttcagtag agcaacttcc tctcactatt aattatactt
    35341 aaacaagtat ttgaaccggt tcatttttaa tatagcattt ttttaaaaga ttgcaacact
    35401 tttttaaaac gctttctgaa agtggcggat tttttgcctt tttaaaattt tattctaaaa
    35461 tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc atccgtttga agttttacat
    35521 tccaatggta taatttcttt atcctatcaa atgggggaag tattttgaag aaattaatcg
    35581 tactatttat tttgtctgcg ggacttttga cattgactgg ctgtgatttg tttggcggct
    35641 ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa aaagcagata
    35701 ccaccaacag tgtatatacg ctaaaagtac gtaaagatat ggatttatcc gcgggacagt
    35761 tgacatttac agataaaaaa atggaatgga cacgcacgta tcagacacaa ggaactgatt
    35821 cttctgaatc atctgaagaa aaaacagtgc tgacagatat caagatcact accaaagatg
    35881 acgaatatat tatttcagga aaagaaaata aaaaagaaac aacagtcata tttacaaaaa
    35941 tcggtaatta ccgtttgaag gatgaggatg gcaacatcta cagtctatag tgccgtctgt
    36001 tggatcttct tttgtttctc cccttctttt ctgctatagt aaagaagcaa gatgattgta
    36061 aaggagacga aatggatgat cgatagtcct gattattgta catgtaataa caacaaaaat
    36121 atccatacag aatttaatga ccaaggaagc ttgagcatat gcgacaactg tcacaaacta
    36181 gtcaaagatt catttaaaac atttgaatct tatcctgttc aaaattatgt aaaaagtaga
    36241 agaagataaa caaatccaag caaaactcac tatggctgat ttttatgaac ttcaaactca
    36301 gcttcttcct ttgatcaagc ttcatcagta aaacaaacag tggatcattt cagtttacaa
    36361 atcaaagagt aaagatttat ttatttcaaa tatttgaata taaaataacc aaacgataga
    36421 aaggcttatt ctacatcacg tagtaatttt tctttcccgt cgtttggttt tttattttta
    36481 gcctaattct tgaccttttg catgctggtt atccgtaaaa tattttccag caggctttcc
    36541 ttccaattca gctaattttt cttggatctg acggatcacc ttttttgcat cttctggatt
    36601 ttctacaaaa tcgaatttat ccccatccac ttgtatttta ggacatacat caaaatcttc
    36661 ataccattga ttatagcggg tgttcaacgt ttcataatag gtatataacg tctcatcgct
    36721 ttctagctgt tcatagtcac gcccacgttt tttgatacgt tctagcatcg tgtccaaaga
    36781 aactttgata tggaccatca aatcaggccg tttttttgga gcaacatcat ctaactcgtt
    36841 tagcatcgta tctaataagc tatcgtactg ctgtacttcg atatccgtta cccgacctaa
    36901 atcagcgttt agatggaaca acaggctatc ttcatagatg gatcggtcta gcacattgtc
    36961 atcgttcact aaagcatttt tcattgctaa aaatcgcttg ttcaaaaaga agatttgcaa
    37021 taaaaaagcg tactgctcag gatttgaata aaagagcggc aatacttcgt tatcttcgac
    37081 attttcataa aatggtttag aatcaatctc ttttgcaagc atatccgtca aactgctctt
    37141 acctgcacca atcgttcctg aaatcaaaat cactgacata caacccttcc tcttctataa
    37201 gtactcgcct acgtaagttt caacaggcaa tgtttcaaaa tcatattcac gtacagcttg
    37261 gtctgatttt aaatacgtat ggagactttg tgtaatcatc aagttcgtag gtaccgtctc
    37321 ttcttgtaca atgatcatcg gttttttcaa catagttgcc acacctaatt cgtaagccgt
    37381 tcctggatcg attgtttgat gctcaaagtc aaggatagca acaacaaatt ctgcatttgt
    37441 cagttcttcc atatcttttt cgtatacttc ctgcgcccat ccagcgctga atagttcata
    37501 attgctttct tggtgatgac gtggagagta gaaacttgtg actgttttgt tttcctccaa
    37561 cgctttttcg atacgactta ctcgatcgat ttgctcctct gaaaaaaatg gtcctgctaa
    37621 atatatttga ctcattattt tgcctccttc aataataact gatttttaat agatactgct
    37681 cgtaattcat tgaccagttc caatggattg ttggaaaaga ttacttgatc caaccatttt
    37741 ttatccataa aatcctcttt tgcaaaagtt tctaataatt gttgcaagcc gctgtaataa
    37801 ccgccaatat tcaatattcc gattggcttg tcgggcgtca gaccgattgc tatccaagaa
    37861 agaagttgtg agaattcctc caatgtacct agacctccag ggaaaacgag gacagcatct
    37921 cccttttcaa ataataatac ctttctttca tcgatcgttt ctgtaggaat aaatgtagta
    37981 acatcttttc caggcaattc agtttcaaaa agcccattcg gatagatacc tgtgactttt
    38041 ccgttatttt ccagtactgt tttactaatc actcccataa tcccggagta tgaaccaccg
    38101 taaaccagtt cgatttcttc ttgtgccatg tattttccta acgtttgcgc aataaatttg
    38161 taactttctt tattaccaaa acgggaaccg caaaaaacag taagtttcat aaaaaaacac
    38221 tccctaactc aaaagtctat cttcattcta cacgtgtttt tttaaaataa aagactagat
    38281 tttgtagttt tttagtggta tataggatag acgccactat atatagtatt tagaaaaaaa
    38341 tcatttttca gccattttat agataaaaca aaaataaata acgtgttttc atcgatatga
    38401 aatgacttac ttatagaaga tactgggatt tcgtcctata tgccaataac gtgtattatt
    38461 ttaaacaaca aatacttgtt tcttttatta acgaaaaaat cattttgttc tcgtgttatg
    38521 tttcactgat aaatgatgtt tctctaaata aaaaagccga cgaactaaac gatcaaaatt
    38581 tgatcatttt tgttcatcag cttctcctaa aaatcttttt gttaacgata aatagctgga
    38641 tttgtaaaaa tatcacttga atcgtcactg ctggaagaaa attcagtgat aaccgcacct
    38701 gttgcgcctg cctggaacta gtgtttgata ttgggctcga acgtgtactg atctctagca
    38761 tgaagaataa tttctttttt tactgtataa taactttctt tcctttttgg aatagtacag
    38821 gaaagttttt ttgtttcctt tccttcgaca taaggtatac cagacccttt cgacaacgaa
    38881 aggttctcat ttttccttca tcgtcatttc aaggagggtg ccgatgctct ggacagtttc
    38941 gatcgctcct aaaccaaaat ctgtatattc gattctttct aactctgatt ctttcatacc
    39001 attcctgtct caaggtacat ctattgtact tttttcttgt aatccattct gcctctccta
    39061 actaattgtc aaatcatccc aagatatgtc ttcttcctct ttttcttctt ggatgatttc
    39121 tgcattcgga tctactggtt ttttcaataa taaaatggtt attcctgtga caaaagaacc
    39181 agctaacaat gcaccaatga atgcccatgg tctggtcatc gtagcgactg caaacattcc
    39241 cgatgccgga atacggctat ctgcgcccca aaacatgctt aatccgccac ctactgtccc
    39301 accgactccc gttgctatga cattgcgtgc caagtcgttc aatacgatag ggaaacatcc
    39361 ctctgtgatc tgacagatcc ccataggaaa agcggtcttc aatgtatcga tttcttgacg
    39421 attgagtata tttttacgaa tgattttccc taagaaataa gcgatcgtca agccgaatgg
    39481 ggttaccatc gaagctaaaa tcaatacagt gattggttca tagattcctt ctgaaaatcg
    39541 tgccacaccc ccttatccag aataaatggt aatccagaaa cagccgtgta tctgcggtaa
    39601 taagcctaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
    39661 atgcagggca gctttttcac aacctctttt tttatttatg tttcataatc aatatggtaa
    39721 ggatgcagct ccgtatggta gaccgcctcc gaatattcaa tcatttcccc aatttgcgta
    39781 gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat tccttgttct
    39841 tcttctgtta aaaatactgc cgagaaatca tcagaaaatg agtcaatagt atatcctttt
    39901 ttcgtcagca aacgatacaa agattctttg ccaaattctt ctctgctgaa ttgcatcatt
    39961 tcttgtggaa gataatgaac aaaataaata taaggctgat tgttcaaata atataagcgc
    40021 ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag gatcttcctt ctttatatgg
    40081 accaattgca aatccagcgt ttctttccgg acttcatatc gggagttctc caagatttgc
    40141 gtaaagcttg ccgctttgga taatcgattg taaggacgat tgcttagaac cgtcgtccca
    40201 cgaccacttt tctttgcgac atattcatct gatgccagca gttcgattgc ctttcggaca
    40261 gtgattttgc tgacttgaaa cagttcttct aattcacttt ctgtcggcaa catcgaaccg
    40321 actggatatt ttcctgagaa aatatcttct ttaattttat ctgcgacttc caagtatagt
    40381 acacgtgtcc ttttcataac gtcacccttt gctttactat tactaaaaga atagcacata
    40441 aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg ttctttttca ttcagctgct
    40501 ttaattacaa gtttacgcca tttgaggcaa ttacttcttt ataccaatca aaagattttt
    40561 tctttgatcg attcagtgtc ccatttcctt catcgtctag atccacatag ataaagccgt
    40621 aacgtttact catttctcct gttgatgcac tgacaagatc gatacatccc caaggcgtat
    40681 agcccatcaa atctacaccg tctgctactg cattagccat cgcttcgata tgacgacgta
    40741 aataatcgat ccgataatca tcaataatcg tgccatcctc ttccatatga tcaatggctc
    40801 caagaccgtt ttcaacaatg aataatggtt tctggtaacg attgtataat tcattcaatg
    40861 cgattctcag cccttctgga tcgatctgcc agccccattc acttgcctca agaaacggat
    40921 tttttactcc tcccagcaag ttcccttctg aagttgctgc ttgtggatca gtctcattga
    40981 ttgccgtaga catataataa ctaaatccga tgtagtccac tggatattct tttagcaact
    41041 ggaagtcttt ttccgtttgc tctaattttt ccggatcgac tccatatttt ttatgggtac
    41101 gcgctgtgta tgcagggtat tctccacgta cttggacatc cgtgcagaaa aaattgaatt
    41161 cttggttttg gatcattgtt gctgcctgat tgactggatt ggcatcgatg ctgtaagttg
    41221 ttgcatagat aatcatgcag ccgatttgga tatccgaacg caattcgtgg ccgattttta
    41281 cggctttgct acttgcaacg aattgattat gccatgcttg gaaaatattt tggtactctc
    41341 ctgctccatt gctttttact aatccttgac ttaaagctgg aaagtgaaag gccgaattga
    41401 tttcattgaa agtcatccaa tatttgactt tactgctgta tctctctaat actgtctgag
    41461 caaatcgttc atagaagtcg atcagttttc gatttttcca gccaccgtat tcttttgcca
    41521 aatgaagagg catttcgtaa tgagaaatcg tgatcacagg ttcaatatca tattttaagc
    41581 actcatcaat cagctgatca taaaattcta atcccgcttc gttaggagta ccttcgtctc
    41641 catttggaaa aattcgtgtc caggcaatcg aaaaacgata acatttgaat cccatttccg
    41701 caaacaaggc aagatcttct ttgaaacgat cgtaatgatc gattcctcga tgattcgggt
    41761 aaatatattt ttcttgatca atcgtccagt caaattcttc actgccgata atcgcaaagc
    41821 gctgcttacc tccaggcata gcatctgcaa cggatagtcc ttttccgtct cgatcataag
    41881 ccccttctaa ttgatttggc agcagttgct ccaccccata aaaatttttc tgaaaaacgt
    41941 gattgcatcc tgatttcctc cttattttac aattgccaga acatcttcgt ttgcaataat
    42001 ctctttttgg tctaattcaa gtatatcggc aaatgcatca gtattcgtaa tgacaactgg
    42061 agtgattacg ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa tcaataaatc
    42121 tcctgctttg acgagatccc cttgtttgac taagatctca aagccattcc catttaattc
    42181 tactgtatcc atcccgatat gaatcaaaat ctccgctccg tcagtcgttg tgatccctac
    42241 agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa ctagttctcc
    42301 tacttctgga tcaatagcga taccttttcc catcacgcca gaagaaaaaa cttggtctgg
    42361 tacttcactc aatggcagta ctttacccgt taaaggactt tttaaggtat gccgtgctga
    42421 agtaatcggt tttcctgcat tcgcatgttt gttttctaat tgattgtctt gtgtttgttc
    42481 gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc ctgtagctat
    42541 tactgctact gtcacatttg actctacacc atccaccgta ttaatgaaag tcggaatcgt
    42601 caataagctg accaaactgc tggagaaagc ttttactccg gaaaatccga taatagctcc
    42661 accaatacct ccagaaatac aagcggcaat gaatggtttc ttcaaaggta atgtcacacc
    42721 atacacagtt ggttcagtga ttccaaataa cgaagtcact gtagaagaaa tccctaatgc
    42781 tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa gcagccccac cttgtgccaa
    42841 gatggctgga agaagcattg gcatcaatac atcaaaccca tattgttcga tattcaaaaa
    42901 catgatcggt acgaatcccc aatgcatgcc gaacatcacg aatacttgcc ataaacttcc
    42961 catgattaga cctgctacga ttggactaaa tccataaatc gagttgaaga gaccgcctaa
    43021 agcattaccg gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg gaaccatcac
    43081 tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca ctttttttac
    43141 gaatggctct aatttacttt gaacccatac ggctagaatg attggtatga cagaagaagt
    43201 ataaccagat ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt ctcccgcacc
    43261 agcaactgct gtgatagctg gatacacaag tgccatagca attgctactg ctaaaaactg
    43321 gttggctttg aactttttag ctgctgtaaa agcaagcatg acaggtaaga aggtaaagac
    43381 accatctgct gcagcaaaca atatttgata agtaccgcta tcagctgtca gccagcccat
    43441 caccgttgcc aatgataaga atcctttcaa taccccggcg gctgccatcg cccctagaaa
    43501 aggtgtaaat atactagaga taatatcgat cagacgattc aaaatatttt tggactcttc
    43561 ttttccatcc tccgaaactg caagaccttc ctcatccacg atcgattgat aaacatctgc
    43621 tacattactg ccaatcacta cttgatactg accgccactt tgtacgactt ggatcacgtc
    43681 cgggtcttct tttaactttt gcgtatctgc taaagattca tcttttaatc tgaatcgcaa
    43741 ccgcgtagca caatgtacta aacttttgac attcttttgt cctcctaccg cttcccatac
    43801 acgacgacca actgcttgat tatccattgt tttttccttc tttctttttt atttaaaaaa
    43861 taaaaaccca gattgaaaaa aacagtcaca aacattcgtg aacctatttt ttcaatccag
    43921 gttttgcctg ccatacagta acaatcctta tgattggatg ctttttttct cacttactac
    43981 tctttgaata tgaatcgtta aataaatctg ctctgcgatc cccatttcct gtttttgcgt
    44041 ttgtcgaaga tactcattga tccgttcgac acaatgaaac gcttttggat actttccttg
    44101 aactaatgcg tagagaaatt catcttgctc gtcagatcct tcccctttga gataacgctg
    44161 ggcaaaaaac tgcagatgag taatgatacg ctgataattc aaagattctt catcgaacac
    44221 gatgccgaag aatttactga tgatactcag tatatctctt accattgtgg tagcggtcat
    44281 cgtcacttgc atgtcttcgt ttccttgttg gctattcaca aaatgaaagg caataaaacc
    44341 ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa gagcctcttt
    44401 cgctgcttga tattctttcg gataaaattt ttttgtttca aataataatg gattaggcag
    44461 ttggatattt tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca atgtcagata
    44521 aatattaggc gaaaattcaa cttttagttt atcttcagcc agttcgacta cttgtttgac
    44581 ccattcgatc tcctctgccg gaatatcagc catcagctgc tggaattggt ttttagcaaa
    44641 agaatctctt aaaacaaatt ctttttggac cgcttctggc gaaatctcgt ccccaatctt
    44701 tttttgaaaa gctagtcctt ttcccataca aacgacttct tccccgaatc catttttcga
    44761 aatgacgaca ttgttattca aaattttatc aattttcatt ttgcttcgcc ctctcaagaa
    44821 aaataaaaaa acctagaatt cgcctactgt ttcttagctg aattctggtt ttgcctgccg
    44881 tacagtaaca atccatttct tattcaaaat catcatacca tcgaattttt atttatgcaa
    44941 gccttttcat ataaaaaata aaaaaactgg aacacaagtc tatctagaaa acaaccaaat
    45001 cagagatgca cctctttgcg tttctctttt tttcagcaaa ttggtttttc cttatcgatt
    45061 catatccttt ggctgttttt aatctgtaac gacttatgtc ccagtatcct aaaatgtttt
    45121 tgtctctaga ttgaaagcag atctcatatc atctgtcgca tcttttatcg atgtaacact
    45181 ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc atgtcgccac aagtaattag
    45241 tgtgacaatt tcttttccct cgacttcatt cagtaattgg gtatctgtag gagcaacttt
    45301 ttctttaaaa aatgttttgt atgtatacac attttctaaa tctgtcaaat agatcttggc
    45361 acccatctct aaattttcta gcggtgtaaa cagcaattca gggttcgttg cacgatggct
    45421 tgctaaggca taatttcctt ttcccatttc ttgatctgga gataatgttc ctgctccgta
    45481 aagtagtgac gtattatcca agcctttgaa aaccggtaaa ttgattgaaa ctgaagggac
    45541 tgcgatacta gcaatcactg gtagatctgt tcctctcatt tgcgacctca tgaccccttc
    45601 tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca tctttatcgt tattcttttt
    45661 cagatcttct tttgttacat tcgctatagc atatttatct cctgtctctc tgacaaaata
    45721 atccttgatc tgttcattga aaatcaaagc tagtcctact aataacaaga ggaacaagag
    45781 gatattgact agccatcttt ttgcttttcc tttttttttc attttctctc cctccagact
    45841 cattttactg tttttctttt gctctttcca acagccgaat gacacatttc tacaactctg
    45901 tgtatttttt tcgtacacct gcgctttttt ctacaggata tttcaaatta tttttgatca
    45961 atttttcttc aatgatctca tctagatcca ggtgaaggtt gtctgccatc atgtatgaat
    46021 agatcaatac atctgccagt tcttctttga ttcgttctaa ttgtgtttct tttacttctt
    46081 ccggcgtttt ccattgaaaa agttctaaca attcagatgc ttccaaagaa atagagatag
    46141 ccaaatcttt ttcgttatga aattgtcgcc aattacgttc atctctaaac tggttgactt
    46201 tttccatact attcatttgc ttgccttctt ccaatcactt cttacttgat taatcatacc
    46261 atttttttac ataaaaaata gatgatgctt ttaaaaaaac tcctgttttc ttttatttaa
    46321 aataaatgtc atagaaaatc aataatttag gtagccacca tgtgtatgca gtagcgtgtc
    46381 ttcttccctt gaggcttttt gtttttcgat acgttcttct tcccaaacag acaacgctac
    46441 tagaatctct cttaatgatt ttccttcttc ggtgagcgag tactctactt taggcgggac
    46501 ttggttatag gcttttcgat ggatactttc atcttgttct aactctctta actgctgggt
    46561 cagcattttt tttgtgatcg tagggatttg cttgcttctc cacgcctaaa ataagcgata
    46621 ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca ttttgacgat cgtgggttcc
    46681 gcttttcact tcttatctac ttagtatcat gaagtatact ttcgtactaa tactaagtag
    46741 cggaagtgtc aactactttg tttaactaca tagcaaaaga aacagaaggg tgacaacggt
    46801 catcacgctt ctgtttcttt tgaatatgct gtgttgttgt ctctatctcc gtgtagactt
    46861 tcctcaaact tatcctttga cagtaagtca gtaggctgat ttgctctttc tagatgccag
    46921 atacatttct acaaaaaaat agtgattctg ttttgaaaat tcgattttta gttctgcatt
    46981 ttcgtaaaca tccaacattt ttttcgcact gtatagacct aacccgccat tttttttaga
    47041 ggtttttcct cttttgacca tctccgatag cgtcaaattc gttggatttg ccttgttcgc
    47101 tatactaaaa cgtgatccct ctttttcttc cagcagattg atcgtaatcg gactctgatc
    47161 ggctacacga tgatcgaatg cgttagaaat aaaaatagat aaacagcgaa taaaaacaac
    47221 cagatcaatc gggattgttt tcacttcgac aggaataact acttggatcg ggattttctc
    47281 cgattctgct ttttctagaa attttaaaaa gacacccctg acagctgggg atacaatatt
    47341 tgttagctgt tgtatttgcg gtttttcaaa aatagctgca gaatgatctt ctaatatttc
    47401 taaaaattcc aacgtttctt ttttattttc ctgaagagta atttttaatg aaaacagtag
    47461 acttttataa tcatgtttga attcctctag atgatggtaa tattcttcat ttatttgata
    47521 cagttgaagc gattcctgat gttgtctttc ttttacttgg aaactgatca acataatgga
    47581 aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga gatagaagca gataattaga
    47641 tgagcggaag tgaacacttc ccaataccaa gtaaatcacg ccccagctta ataaaataca
    47701 aatacctcca aaatagtgtt cagttggata agtaaaaagc aagttgagaa atctcgtctg
    47761 tttgtctata ctttttacta atagatagaa aataaatgcg ataagtagtg caaaaaaact
    47821 tgctgttcca agatgtctct ttcctaaata atccatcaga tcaaaaacca gaaaaaagct
    47881 gagtccagtg atagcaaaaa aaagattact gatcaaacag ctgataaaca ggtttttcgt
    47941 agtaaaataa aaaagaaaca tcaataataa gccaactagg taaggaaagc tcactttagg
    48001 gataggaagt aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt tttttactag
    48061 cagttgtttc gaaaagaata aatagatacc taaatctaaa atcaaagtaa aggtcaaata
    48121 aagtgtcact gttgcgacca tcttattcct ccattgtttt gtttcaaaat gtacacatat
    48181 ttttttaata ggagcctgca tatgaaaaag tattttatca tagaagataa ccagtttcac
    48241 agcgaatgga tcatcagaga attggtcaat catcatatcg agaatatcgt gcagctcact
    48301 tctatctctc aaatcaatca gttgatccag ttgggcacta ttgataatca cgatgttttc
    48361 tttatcgata tcgacttgaa gcaatcactc acaggtattg atgtcgccga acaaatacgt
    48421 aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt atacgaattt tgcagtagag
    48481 atcatcaatc ggaaaatcct tccgattgga tatatcagaa aagacaggaa tttatctgaa
    48541 caaattcgtc aagtgctaga acagatcaat tggaaagaac agcagatcgt aaatgtcgat
    48601 aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga aattttatat
    48661 attgaaagta tcaaaggttt gaaaaaccaa gttatggtca aaacaactac agaagataaa
    48721 ttagtgaata cttctttaaa acaattgaag acagagttag aaaagtatgc ttacttttta
    48781 cttttaaaat cttactgtat caacttatcg caattgcaga aaattgattc tgctcattat
    48841 gtgttagaat ttttcaacgg tgactcctta ctcgttggtc gaaagatctt tgaaaagaca
    48901 aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt gatgaataag agctgagacg
    48961 taagcatagc agatttcagg gagtgggcct tgaatcggct ccactccctg aaatcgttga
    49021 tatgatttta cgcttcctct tctgtaggag catctgttaa cgaccatgtg aatgttgaaa
    49081 cgtagttgtc tacagcataa ttggatacag gaacaaataa ttccactgat tgtctccctt
    49141 gttccacatt ctctcctaat gaataaatcc atgtgccaat cccttcattt gctcctgcag
    49201 ttactagatc tactgcctga tctgctggga tttcgattcc ttcagcgcca gttccagaaa
    49261 cggtagatgg cattccactg ctattagttg cgctatttcg aatcacggcg tttttgaatt
    49321 tcacctttga accagttagt ttattcgtat cagatgttgt accaataaat tctgttcgtt
    49381 tgactgataa tttccaaccg gcataatttc cgcttttatc cgttacttgt acgaaattct
    49441 gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg taatgcagca tatactgttt
    49501 ttgctgtacc taacttgatt gttccaaaat caagatctgg agcttggtca atcgtcagta
    49561 atccggatgt tccctgattt ggttcgtcag gtgtaaaagg agtatcagga tcatttggat
    49621 caacaggagg aactggaaca acgtcttcag gatttgcagc atctggtttg gtcacagaaa
    49681 tcgttccttc tgtagtggct gagctcttat tctcagcaaa aacactttgt gtacataata
    49741 atagacttcc agctagaacg gtacttaatg caattgtttt tttcatttct ttttccttct
    49801 ttcttttaaa ataaataatc cagcaaataa gccaataaga atgactccaa caagtatcag
    49861 ccatcgccag tcaaatgttt ctggctgatt ttttaaatca atatcctttt cattcaattg
    49921 gtccgctgca tcatcagtta tttcaaaatc ggtatggaga ttccagcgtt tttctttcca
    49981 agcacccttc acaaccgctt gataatgtcc cggagctaat ttttttcctt ctaatgggac
    50041 aagtaaatca aaatttgtat tgggtgctac atctattttt tcttttttta ttgaaaggta
    50101 aggctctttt gtttcttttt tgtagatttc aaccgatgta gtcaagccga acatataaga
    50161 agcatttgga ttttgaagat tcgccttaat cacatttctt ccatcgcttt gatccgcata
    50221 agcttttttt aatttcaaat ctggggaaga ctctttttcg ctatttcaaa tcaagattgc
    50281 tttcacttga gaaaactgat tgacgacacc aactgtactt ttgttagaaa cgttctcttt
    50341 tttttcttga aacgtgatcc ctcctgcgac cacaccagcc agctcttttt ctggcgcttt
    50401 cagtgaaagt gtctcagtga ccgcactttg aggaggaaca gttatttctc ccactgtttt
    50461 tgctaagttt ttcatatcat aacttaatgt ttcatcaaat ttactttttg attctccata
    50521 ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc aatgtctcaa ctgttactga
    50581 tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt gttttcccag gctccatcct
    50641 tacatttata tatgttttgt tcttatcaat ctgattagcc ggtaattcaa aatctaccgc
    50701 aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg atgaatccaa ggagaaggat
    50761 cataagtaaa gagcagttgt ttcgatgact tttattccat attcgattca tcaatcatcc
    50821 tccttgatct ttattctgtc ggcgcatctg ctaatgtcca tgttatctct gctgagtaat
    50881 taccagctat ctgattttct ttggttaaga caagtttaaa cgcccgaccg ttatttaaat
    50941 attctgaata atctgcttgc acattcgttg aattttgtcc tctgacaatt tccagttctt
    51001 cttcattcac taattgatac tcctctcctt tcttgaaata taggacatcc tgcatagaaa
    51061 atcctgcatc atttgtgaaa ggctgagata attttatcgt taagatccac ggatttttcg
    51121 tttctcgctg atcataaatc cataattttt cctgtatatc catttggcca aatgcttctt
    51181 gtgtcaaacc agttgctttg atctgaccaa aatcgagttt ggaaggggca agaaatgaga
    51241 gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg cggggcatca tgataaacaa
    51301 ctggcgtatc acttggattg tgattcccaa tttcatcgtt ggtacttggc ttgttctgca
    51361 cacccttagc agtagcttct cctgcttggt cgtttaatac aatttctaat acatctcctg
    51421 caaaaagttg attttctggg atagaaagaa caaattcccc tgtatcatca atagttgttt
    51481 ctgacaaaac agcttgattt tttttcaatg aaaccaaggc tcctgcttca cctgtacctg
    51541 tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa attagcaggt tcaggaggaa
    51601 tcacatcgac tgtcgttttg gcagagtcca gttctgtcac ttcttggcca cctcttactt
    51661 ttttagcagc aacaacttgc acttgatcct ccgtttttaa tctctcatca ttgggtactt
    51721 gaacttcgaa caagccgcct tgttcgcctt ctccccatgg gttatatcct gccccttcat
    51781 tttcatctgt ttgtccttgc gtgagagcag taatttctac agatgatttc gtcgtatctg
    51841 cgtaagtaat acgtaaagtc acttctactt cgcctgtcca agcatctctt ggctttgcct
    51901 cgacaccttc gggaacaacg acatgcccat agattttttt atctgcattc gttggcactc
    51961 ttaaggattc aatcatcggg agctgattat tggcactgat tcgattataa tttttcattc
    52021 cggcacctgt ctgagcaaat tcatttgtta aacctgcact agggcttgat acgaaattgc
    52081 ctaagtcggt cccattgatc gataagtctg tcatatcaga aaaataatcc gggtcttttt
    52141 caaagttatc gaatgttgcg cctgtttttc gccataaagc gatttctgaa tgggcgatct
    52201 ttaaagaaga atctccattt ccttggaata cccagccgcc tatgccattg gtctcgcttc
    52261 caatccgttt gttttggaaa tcaaaatagg taggagattc taagtccaca ttcaacacat
    52321 ccccactgat gatgccggtc ttatcttccc ctgttttccc gaccatgact aaataagttt
    52381 tttcacctgc cgtaaaattt aaatcaatat ctccagttgt atctacagtt gctccggaat
    52441 ctgcgcgtat atcgatattt gatttttctc cagtgactgt aaaatcagct gaacctggac
    52501 tttctagaaa accagaagcg tcataaaatt ggatcgcttg atttccttga ttgagtctgt
    52561 tcacgtaaga tttgccatca cctgtatttc ttaccctcaa tgagcctcca ttggttactg
    52621 tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga acgcccactc tgcttatcga
    52681 tgtcgataac tgatccggct ccttctacga tcaaagaaag gccttttcct acaaaacgca
    52741 tcccattgtt cccgggttga tcgtcatctc cagccatttt gaccgagatt tgtccaccac
    52801 ttgatacatg gatttgacta gaatctcctt gcaagcgaaa tccgcccgta gttgctgtat
    52861 tgatggtgat tttcccacca ttcgatacag tgactttcga tcctgaacct ctcatttgga
    52921 tgattccgcg attgttgtca ttggtaatat tgatctttgt atttaccgaa aattcgcttc
    52981 ccgaaccact gacttcaaac atgacattac caggtagcgt atcgactgcc tctccttcat
    53041 cgctaacgcc atctgcccga tcataatcaa gagagacctt tgaaccgctt aagatatgga
    53101 ttcctggcgc tgtttttcca ctaccagtct catctcctga atctagaaaa ccgtagaatt
    53161 tccctagata cggtccataa agttgactac cattgatcac attcaattga tagcttctac
    53221 aaaaataggc tttacccgga gcggcattca gtccttcctt cactggttgg acatccaaca
    53281 caccggaaac accatctaat gtgacagttg cacgaggagc gtaaatcagt cctaatttgg
    53341 acccatcata caaatcaagt gtcccggtaa attttatttc tcccgtaaaa tgatatctgc
    53401 ttccccaaaa atcggtcata taagcaatga taatggcatt tccatcgata tttggtgtca
    53461 atccacctgg atgtgcagct gttcctttaa aattctggaa ctcgatcgac tgaggtatct
    53521 cattgatata aatatggtgt gtgccgaaat tgtgagcgaa tccaccaaaa tcaataacga
    53581 tatctcgttt gatctctaat tccctgtcta aagtcaaagg ctgaatcagt cggatcttag
    53641 ttactgattc gtctgcgaga agctgcgcca gttcttctgt tgtggcagct tcgctgtacc
    53701 catccgctga aacagctctt gccagtgccg cttcactgct atcagacgtt tgttcagcta
    53761 cttctgaggt ctcacttgtc ttattttctt cttgcctttt attagtagaa gcctcacttt
    53821 gagattctgt agtttcagaa gtaatcgatt cttctgaaaa agtagtcttg tcaaaacttt
    53881 gattctctga ttgcgttgat tcgtttgttt tctctacatc ggcaaaaacg gatgtcacca
    53941 tcaagagagg gaagacaaac aaaatcgacg caatcagctt ttttacacat tgattcattc
    54001 tccaccatcc tctctgacgt tttgacaatt ttattttatc gttttaatct atttccaatt
    54061 gtactttcat atttaaaata tgtattttta tggaaaaatt tttctattag gtgtaaattt
    54121 cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc acagtccctt atcccgaaca
    54181 agcggtgttc tcccgccaac tcctcggtca caacctccat tttaacaata aacctgtaaa
    54241 tgtgagggca atactttgat tgtcacaggt aatttatccc cttcatctcc atcaacattc
    54301 gtcgtcaatt cttcctcatc agctaatgac acaactaatt gctcacaagt aaggtaggct
    54361 agatttttcg tagattcatc tactcctttt aataggtctg gcaatgtttt aagcgtatcg
    54421 atcaaagaag aatcttttaa ataaaccaaa tggagcttac catcatccac ttttgcattt
    54481 ggaagtaatg tttcaaaacc gccgactgag ttagttagtc ctatcagcaa ggtactgctc
    54541 tcaatttctt ccttctttcc atcaacttct acgtgaaacg agtaggattg tgtggaagct
    54601 aactgtttga ttcctgacat gaaataagct agttttccaa atttcgtctt cttctccgca
    54661 tctacatcat tgattgattc agggatagag ccaattgcga cgacattcat aaaatagtcg
    54721 tcatttattt ttccaatatc caatggcttg actgattcaa tcgaaaaatg attgatcgct
    54781 tcctcaggtt ctagcggtat ccctaatgct cgagccaagt cattcaccgt acccaatggg
    54841 aaaaatccga aatttggccg atgttcttgt tcagcgattc cactgattcc ttcgttgact
    54901 gttccatctc cgcccatgac aaacacacta tgataccctt ccgtagccgc ttcgcgagta
    54961 aagttttttg catcccctgc tttttttgta tgcaagacga ccacttcatc aaacaccgat
    55021 tccaattttg ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc tgaactgggg
    55081 tttaccacta ataaaacttt tttcatcact ttctttccct tctgattgat ttctttaata
    55141 taacgatagc ataaagaaaa aaattaggct gtgatgaagt actgttcatg aaacattcat
    55201 ttattttttg acaagataaa acatccatac tatgaaactg aggaaaatta aaacaatgat
    55261 ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa tcttaggtgg
    55321 cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact ttttgttatc
    55381 agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt gatgtactgt
    55441 taatatgtca cggccttgaa taataaatgc gacaatcccc gctataatta gtgtgcctaa
    55501 caccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg tacttcctgc
    55561 ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa ataaaagaaa
    55621 tttgcaaaac cactccattt atctacttct acgtgcattt tttgatattg ttttctt
//
LOCUS       DASJOX010000007        51301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJOX010000007 DASJOX010000000
VERSION     DASJOX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.007"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1029)
                     /locus_tag="U4A12_000374"
     CDS             complement(<1..1029)
                     /locus_tag="U4A12_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1772787.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKIN"
     gene            complement(1306..3108)
                     /locus_tag="U4A12_000375"
     CDS             complement(1306..3108)
                     /locus_tag="U4A12_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772788.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3122..3874)
                     /locus_tag="U4A12_000376"
     CDS             complement(3122..3874)
                     /locus_tag="U4A12_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772789.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3883..4245)
                     /locus_tag="U4A12_000377"
     CDS             complement(3883..4245)
                     /locus_tag="U4A12_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1772790.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4421..5293)
                     /locus_tag="U4A12_000378"
     CDS             complement(4421..5293)
                     /locus_tag="U4A12_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1772791.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5319..5831)
                     /locus_tag="U4A12_000379"
     CDS             complement(5319..5831)
                     /locus_tag="U4A12_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN1772792.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(5969..6760)
                     /locus_tag="U4A12_000380"
     CDS             complement(5969..6760)
                     /locus_tag="U4A12_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN1772793.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6815..7423)
                     /locus_tag="U4A12_000381"
     CDS             complement(6815..7423)
                     /locus_tag="U4A12_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN1772794.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7416..7733)
                     /locus_tag="U4A12_000382"
     CDS             complement(7416..7733)
                     /locus_tag="U4A12_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1772795.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7889..8617)
                     /locus_tag="U4A12_000383"
     CDS             complement(7889..8617)
                     /locus_tag="U4A12_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN1772796.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8776..9657
                     /locus_tag="U4A12_000384"
     CDS             8776..9657
                     /locus_tag="U4A12_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1772797.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9894..10937)
                     /locus_tag="U4A12_000385"
     CDS             complement(9894..10937)
                     /locus_tag="U4A12_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1772798.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10940..11608)
                     /locus_tag="U4A12_000386"
     CDS             complement(10940..11608)
                     /locus_tag="U4A12_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1772799.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11750..14086
                     /locus_tag="U4A12_000387"
     CDS             11750..14086
                     /locus_tag="U4A12_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1772800.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14201..14512)
                     /locus_tag="U4A12_000388"
     CDS             complement(14201..14512)
                     /locus_tag="U4A12_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772801.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14693..16168
                     /locus_tag="U4A12_000389"
     CDS             14693..16168
                     /locus_tag="U4A12_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN1772802.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16215..16799
                     /locus_tag="U4A12_000390"
     CDS             16215..16799
                     /locus_tag="U4A12_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN1772803.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            16801..17220
                     /locus_tag="U4A12_000391"
     CDS             16801..17220
                     /locus_tag="U4A12_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN1772804.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(17501..17785)
                     /locus_tag="U4A12_000392"
     CDS             complement(17501..17785)
                     /locus_tag="U4A12_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN1772805.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18137..18847)
                     /locus_tag="U4A12_000393"
     CDS             complement(18137..18847)
                     /locus_tag="U4A12_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN1772806.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18844..20316)
                     /locus_tag="U4A12_000394"
     CDS             complement(18844..20316)
                     /locus_tag="U4A12_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1772807.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20454..20927
                     /locus_tag="U4A12_000395"
     CDS             20454..20927
                     /locus_tag="U4A12_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1772808.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21080..21631)
                     /locus_tag="U4A12_000396"
     CDS             complement(21080..21631)
                     /locus_tag="U4A12_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1772809.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21736..22266
                     /locus_tag="U4A12_000397"
     CDS             21736..22266
                     /locus_tag="U4A12_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN1772810.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22749..23192)
                     /locus_tag="U4A12_000398"
     CDS             complement(22749..23192)
                     /locus_tag="U4A12_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1772811.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23208..24368)
                     /locus_tag="U4A12_000399"
     CDS             complement(23208..24368)
                     /locus_tag="U4A12_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1772812.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24875..26575
                     /locus_tag="U4A12_000400"
     CDS             24875..26575
                     /locus_tag="U4A12_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772813.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26677..27510)
                     /locus_tag="U4A12_000401"
     CDS             complement(26677..27510)
                     /locus_tag="U4A12_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN1772814.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27814..29979)
                     /locus_tag="U4A12_000402"
     CDS             complement(27814..29979)
                     /locus_tag="U4A12_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1772815.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30239..32533
                     /locus_tag="U4A12_000403"
     CDS             30239..32533
                     /locus_tag="U4A12_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1772816.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32526..33191
                     /gene="pgmB"
                     /locus_tag="U4A12_000404"
     CDS             32526..33191
                     /gene="pgmB"
                     /locus_tag="U4A12_000404"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1772817.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            33184..34191
                     /locus_tag="U4A12_000405"
     CDS             33184..34191
                     /locus_tag="U4A12_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1772818.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34213..35232
                     /locus_tag="U4A12_000406"
     CDS             34213..35232
                     /locus_tag="U4A12_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1772819.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35513..36880)
                     /gene="nhaC"
                     /locus_tag="U4A12_000407"
     CDS             complement(35513..36880)
                     /gene="nhaC"
                     /locus_tag="U4A12_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1772820.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(36990..38411)
                     /locus_tag="U4A12_000408"
     CDS             complement(36990..38411)
                     /locus_tag="U4A12_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1772821.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38654..40531)
                     /gene="tdc"
                     /locus_tag="U4A12_000409"
     CDS             complement(38654..40531)
                     /gene="tdc"
                     /locus_tag="U4A12_000409"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1772822.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40822..42078)
                     /locus_tag="U4A12_000410"
     CDS             complement(40822..42078)
                     /locus_tag="U4A12_000410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN1772823.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42190..42395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42658..43959
                     /locus_tag="U4A12_000411"
     CDS             42658..43959
                     /locus_tag="U4A12_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1772824.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44028..44627)
                     /locus_tag="U4A12_000412"
     CDS             complement(44028..44627)
                     /locus_tag="U4A12_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1772825.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44881..45762
                     /locus_tag="U4A12_000413"
     CDS             44881..45762
                     /locus_tag="U4A12_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772826.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45775..47385
                     /locus_tag="U4A12_000414"
     CDS             45775..47385
                     /locus_tag="U4A12_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN1772827.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47462..48121)
                     /locus_tag="U4A12_000415"
     CDS             complement(47462..48121)
                     /locus_tag="U4A12_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1772828.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48404..49069)
                     /locus_tag="U4A12_000416"
     CDS             complement(48404..49069)
                     /locus_tag="U4A12_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN1772829.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49216..49821)
                     /gene="dhaL"
                     /locus_tag="U4A12_000417"
     CDS             complement(49216..49821)
                     /gene="dhaL"
                     /locus_tag="U4A12_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN1772830.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49849..50859)
                     /gene="dhaK"
                     /locus_tag="U4A12_000418"
     CDS             complement(49849..50859)
                     /gene="dhaK"
                     /locus_tag="U4A12_000418"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN1772831.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(50834..51280)
                     /gene="dhaM"
                     /locus_tag="U4A12_000419"
     CDS             complement(50834..51280)
                     /gene="dhaM"
                     /locus_tag="U4A12_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN1772832.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT        15350 a        10619 c         9146 g        16186 t
ORIGIN      
        1 atttattttg tattccgtta caaactcgcg tacttggtta ggttccagag tgacatctcc
       61 aaaactttca agtcgctccg ctcctggcag ttcttgagtc tcaaatgtga taccgccatg
      121 atttactaat ttattcccgc gcatttccgg tccatcttct ccaaaatttg ctgtaaaaat
      181 cacgattcct gaagcatctg tttttacttg gatcgatact ttttcatctg ggcttgtcaa
      241 ttccgcagca tttagtaaac cagattgttt tttcaacaaa aatggatgat cgatgccgcc
      301 cactatcatt gattggcgaa aatcactggc aaagacctca cctaattttt taggacgttg
      361 gaagtcaaat gctgtgccac ttactgaaac tacttctcct gtaggcgttg tatctttatt
      421 gagcgtagca aataatcggc tattcaacca aagtatatgt tcgtcgattg actgtgctgg
      481 atcaccggtt aaattgaaat agacatgatt tgtaggattg aatactgttt tgcggtcact
      541 gatcccttgg accgtcactc gccatgtatc atcgttcgtc agcgtatacc gtacttctac
      601 atctaaagtt ccaggaaatc cgtttcccat atctgggcta gtctgataga aaatgaccga
      661 tgcttcgttt tcaccattga tgactgtata atgccactgc ttggcttcaa agctaggtgc
      721 accaccatgt aaacaatgac cagtgtccgg gtctgttttt acttcgaacg tttcatcatc
      781 taatttgaat gtgccattcg taatccgtcc agccatacgt ccgattgaag caccgatata
      841 agggtctttt tctagatatt cttttgcttc atcaaaacct aagatcaact ctcgtttttc
      901 attatcgatt attgtttgaa aatgaacaac ccgtgcccca agatcactga cagacaaaga
      961 tgtcccattt tgattcgtga tcgtaatcaa gtgatagttt tcgccaaaat tttgttcatt
     1021 gatttccatg ttccttgcac cctcttttct ttcatttgca ctgattccag tatacatgga
     1081 aaccgttttc ttaacagctt attccgtgag taacgagaaa aaatttacta tattctaata
     1141 aaagaccgtg aaaaaaaggt tgtgacaaaa atattgtcac aacctctttt tccgaacaaa
     1201 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgaggagc
     1261 tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca atctcttttt
     1321 caatacgatt ccagtatata accatgtcgt cagtacatag tagatgaaat acaatacgaa
     1381 gaagatcgta aacggaaccc aaatatcttt gtatggattt tgcatgaata gaccaaacat
     1441 ttgtaatccg aataagacat ggactacacc taagattcct ggtgctaaga ataacacgcc
     1501 tacttctcta cggatcgatt gtttaagcaa cccgcgtcgt gttcctatct tttcaagcat
     1561 ttcataacga gcaatgtcac tcttgcttcc tgacaagatc ttgaacatca aacagctagc
     1621 aagcatggtc aagaaagcta aacctaggaa gaatcccata aattcaaaac cagagaatag
     1681 accattatac acttcataca tttcatattt ctggttgctg tactctactt tttgcaaaga
     1741 tggattgttc attttatttt gagcaacaag tgtttcgatt ggtttcaggt ttgcttggaa
     1801 gtcttttacc tgaactagct gtaaagtgga ttgtggcaag ttcagttgat caaattctga
     1861 ttgactgact accttgattt ctttttcttg ctgttccggc aattcatagc ttcgaagttc
     1921 ctcttgtaca gtcagttcat tcattttttt agcaggaact ttttctttcg tgatcttcgg
     1981 cggtgttcca tcttcatgtt ttatcatgat caatggttga gccgcaaatt cacttgcatt
     2041 atagtaaatc gttcccgcat cttctttttg agtataagaa gcagaaagtg ttggtgaaag
     2101 ttctttgatt ttttgctgat cttctgcccg aggattattc atcactagat catatccaga
     2161 attgttttct gtcaagtctg caatttcatt tttaaaacct aatcctaccg tcaaagcgcc
     2221 cagtgccaag gcaaacagca tagcgaccat tgagagcatt tgtgtatatt cacgaatacg
     2281 aaagtttaac tgtgacaatg tgaaattgtt caattttttc aatgaaatct gttctgattt
     2341 ctttagtaat gataaaaaga aaatgatcac agaatggaaa atgaaataag ttcctaatat
     2401 gatcgtaact aaggctaccc ctataccaaa gacttgatag gtccccacgt gtttcatcat
     2461 atagtaaccg cagcctagaa aagcaattcc cagtactact tcaaggaaaa acaagaattt
     2521 atgctgcttg attcttgttg gtgtctgatc cgcacgaatc aatgctaaaa tcggtttttt
     2581 gacaatcgaa aaagcattga caacagcagc taataaaaac aaaacagcaa aaaacaaaac
     2641 agtgatcaac aacccgtgaa tactaaaagg tgtaaaatgc gaaatattga gatctaactg
     2701 atccactaat aatcggtgga cgatacttgt caatcctaca ccgacagctg aacccactaa
     2761 agtagctgcg atccctacta agaatgtttc agaaaaaatc atctgtgcga tttttctgcc
     2821 ttttgctccc agcatcataa acattgcata gtctttttgc cgcatcgtca tcaaaaagga
     2881 atttgcatat aaaatataaa cgaatgtgat gatccctagt aaaacagaac cgaatcggaa
     2941 aatgataact gtagccgcaa cgatcgtatt acttttcaaa aaagcttcgt tgcttgccat
     3001 tgattcaaac atgtaaaaaa tagcagatgc cataactaat cctgagaata acaccaagta
     3061 gtctcttaaa cggctcttga tcccagtaag tgataatttc caaagcatag tcaatctccc
     3121 cctactgttc caagtttcca agaatagata gtatttctcg atagaaggaa tcacggcttt
     3181 gatctccacg tttcacttct tgatggatac cgccatcctt gatgaataaa atacgttggc
     3241 aataacttgc agaaaacgga tcatgcgtca caagaagaat cgatacatga tcttttgtat
     3301 tcaattcgtc catcgtatct aaaagatccc gggcactttt ggaatccagc gcacctgtag
     3361 gctcatcccc taatagaatc gttggttgtg tgatcaatgc acgtgcggcg gcgacccgtt
     3421 gtttttgtcc accggaaatc tcagaaggat agctttccaa aatatgagag atggataaac
     3481 gttctgcaat tttttggaca cgttgcttga tttcttttgg tctgacgcct tgtaaagaaa
     3541 gaggaacagc gatattttct tgtgctgtca agctttctaa taaattgaaa tcttggaaaa
     3601 taaagccaat ctcattcgca cgaaaatctg ctagttgatt tccttttaat gtggtcacat
     3661 ctttttgatt gattcggata tgcccatctg ttggtttatc taatgtggat aaaatattca
     3721 aaagtgtcga cttaccagaa ccagaagcgc ccatgatccc aataaattcg cctttttcta
     3781 catcgaagct gatgccgctt aatgcttgcg tctttttctc attgtttttt ccgtatactt
     3841 ttgttacatt ctgtacttcg actattttgt tcatttcttt ccctaccctt taattagttt
     3901 ttccagtgct gttttcggca cgtctttttt ttcgacttct tcatacgaag tgacaccttt
     3961 atttttgttc caggtgattt ttaaatatgc tcctttacga agcggtcgag tcaattgtcc
     4021 cttgaagtcc agttcttttt ccttaccctc tttcgtatag ccaggcagga tatatctata
     4081 gttgactgtt tcttgtccag agtctgattt tgaaactgtt tttgtaccat ctgtcgtaat
     4141 ttgtacatag tattcttctc cacccataac tacgtaatct gcgattttca cccctgcaag
     4201 taccacaacg atgaaaacaa gtagaccgat cagaagtttt ttcacttttt ccccttctct
     4261 cttactttat atactgatta tagaaaaaag cgaacaattc tgccttcgaa tcaactatca
     4321 ttcttctttc aattttgtca ttttctttaa aacaactaaa aaaagagttc aagacaataa
     4381 tccgtcctaa actccttctt cctatcttac aaaaagtatg tcacttacgg tctttcatgt
     4441 cgcctgtatt gataacaggt tgcgttcctt tgtctgtaat gattgagaca agtaaattat
     4501 tgatcaattg gactttccga tcatcggtga aattgatttc ttgtccatct tcgatttgct
     4561 ctaaggccat ttgagtgatt gtgacagctc cttcaacaat ggtttgtcga gcagacaaaa
     4621 tggcttttgc ttgttgacgt tggagcatgg cactagcgat ttctgttgca taggctaaat
     4681 ggttcaagcg tgtttcgatt acttcgacac ctgctactgc taaacgctct tgcagctctt
     4741 tttgcaactc gtcagacact gccgtcgtgt ttcctcgaag cgtcaaatca ttctcattga
     4801 atgtatcata cgggtattgg ctagcgatat gccgaatggc tgtctcactt tggatttcta
     4861 caaaatcttg ataatacgct acatcgaata atgcctttgc tgtatcaacg actttaaaga
     4921 ctactactgc tgagatttcg atggggtttc catccaaatc attgactttt aaaacagaac
     4981 tattgaaatt ccgaactttc aacgaaacag tcattttttg tgccaaaggt atcgtaagga
     5041 aaaagccatt ttctcgaatc gtacctaaat attgtccaaa aaacaaaatt acttttgctt
     5101 gattaggact gactacagtt gccgaactta acaacaataa cgtgatcaac caaagaaaaa
     5161 tacttaaaaa aagtgcccaa actttcgctt gccacatacc taaatagaag aaaaacaatc
     5221 caactaaagc taatacgacc aatccgacaa tccctgcata accattgata taaaatgctc
     5281 ttttttctct catgctactt cctgcgctcc tttatccttc aataaatatc taatgttact
     5341 tttttaattt tttttcgaat acgcgccatt acaccttcta gttctaactc tttgtcttca
     5401 gtgatgtacc aaccccttgc ctgtgtccct ccggctgcct gcggtaaata atccaatttt
     5461 gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta ccaagacctc tagatccatc
     5521 cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga taaatcgttc cttcttccca
     5581 tcatacttca atattcctgt atcgataatc ggtaaagaat aataatccag ctctttcttc
     5641 accgtcaata aatgtttgaa aacttcatgg tacacttgct ctaattggcg accttctgaa
     5701 ttgttcgggt aattaaccat cactttttct ttgtctggat catagaaggt ttggcttagt
     5761 tgagtgatat aagtatgtaa tctttccatt tctgttttta acttttctct ttgaccttct
     5821 tctaccttca tatactcttt caccctgttt tctttttaga tattttcttt cttattttac
     5881 tatagttatg tgcgttaagc gatgaaaaac agcaaagatt ggaaggatct ttgctgtttt
     5941 ttcttaaaat atccagttga gtacaggatc aatctgtttc attgtcctct tgatccgtcg
     6001 taatattgat cgatgtgtct ttagcagtga tctttagctt tttctgtcct tgttctaaca
     6061 aagtatttgt tattggctgg tagtcttttt caatataaga ttcaccagac gtttggatag
     6121 tatagccact gttttccatc tcatgcaaag ataaattgcc accgtttagg atgacttttc
     6181 cttctttgct gatcaaactg tcttctagat ggaagttgct gttttcacct gtgacgatca
     6241 agttatccac attggcctca tctaatcgaa gtgagcctaa ttcatctttc gcagttattg
     6301 ttcctcctgt aaattcattg aacgataccc agccatcatg tgtcttagta ttcacttttt
     6361 tgattgatac ttcgttgata tcaattgcac tattattccc ttcgattgtc aattcagata
     6421 attcatgatc tgcagggaca acaatagtga tttgtgcact tgtgtcttct aaactaatga
     6481 ttgcacctgt tgattttttg actgcattca cagtcaacac gccatttttc tcctttattt
     6541 ctggcttttc tgtcttatca taagtcgttt ttatctcaaa atccaatccg cgtatgaccg
     6601 aaactgtttg attttctgca tggatgacga ttttgtttgc atccttgatt ttttttgtct
     6661 cagtaaccat atcgatcaac cgaggaccat tatcccaagt cagactcttt tgagcgccta
     6721 atgtataagt tgcacctgct aggaggccac cagtaagcat cagaccaata ccggtaatca
     6781 aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac gcccgctgaa tgcagatctc
     6841 tttttgtaat aaaacgtcgt cccatccatt taacaaagtt catcatgaca taaaaaagcc
     6901 cttttgttcc tttcagcaac agtggcgaaa taaatactgc gcttcctgtg gcgaacagac
     6961 cgacaccgat aaagaataat ccactcagaa tcgattgtcc aaacacactg attcctgaga
     7021 tgatcatgaa taggccaaca cctaagatac tgatacataa aacatagata ctaaaaatga
     7081 gcgctagtaa agcaaccaat agtcccccaa taaccaatat aaaccccagt gctagtggaa
     7141 tgaggacagg agaagcaaat aaaccgataa tgattaacca aatcaggcga atttgtcttt
     7201 ttggattttt cactaactcg ttttctgctt gttcttcttc tgaaaagtaa ctgattttta
     7261 attttaaggc aaactgcttt ggtgtaccat attcatcaat gatctgatct aatgtttttc
     7321 cagaatctaa aaaatattcc tcataatact ggatcaaatc aaaacgatcg tcttcaggaa
     7381 tttcttgaag atgatccaca acttcattca aatattcatt catttgtgct ctcctccaat
     7441 aaccggtcta aagaatctcg aaaaacaatc cattcatcaa taatttcttc taaatgacga
     7501 cttcccattg gtgtcaacga gtaatatcga cggtttctgc cttcaaaagg ttcatcatag
     7561 gtctcaagat atccttcttt tttcaatctt ctcaaaaccg gatatagtgt agattcactg
     7621 acagagagac tttcttgtac gatttttgtt aaggcatacc catacatatc ttccttgctt
     7681 aatgctgcta gcacagtacc atcaagaaga acagttggaa tctggatgtg cattacgtca
     7741 ccttctattc tcaagtgtaa tattttattt caatctatat tatacaacat ataatatagt
     7801 ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa gatttgatcc tttttagtca
     7861 gcaaaaaatg gcttctcttt gatttttatt ataacagtgg cataatccaa tttgtccaca
     7921 tccagctcag tggcatgata acgatcattg caggtatcat gtcagctact gggaacattt
     7981 tcacgttgat cattctaaaa ccggtagcga ccatcaagaa tccgccgcaa gctttaaaat
     8041 ctaagatcat gtctggtgta gtcaatggat aaataaattt tgccaataag aacaaaatat
     8101 aaaaaataat gaattgggga attgctatca cagaaacgac ataccctaaa ttacacgcaa
     8161 aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat caaaatcgta ctgtctcctg
     8221 tcatcccaga atctaaactt ccatagatcc ccgtaccact tgcacaaaat agaacaatga
     8281 ccgtcactaa tgtgctgata aattcatcgt gggtcatacc aagcttttca ctagggaaga
     8341 ttttagaaat aggtacttgc atcagcgcag cccccttatt tatcccatta cctaaatgaa
     8401 caatcagacc aatcgctgtt cctatcacta aagcaaaaat aacagccggc atatttttca
     8461 tcggcgcaat ggaatagatt cccatcccca tcgaacacac accaaaaacc atcgtcagct
     8521 gtgctttaaa atgttcactt aatttatttc ctactaatcc gccaattagt ccgccaagca
     8581 aaacggaacc tacattgatc aaaactccag ttggcatcaa aaaactcctc tctttttcta
     8641 cgaagcgaaa aaaatcagaa aaatttctgt aattattgct ttcgctgcaa gaaagattgt
     8701 aacagacaga gaagattaca ccaattcatt ttttgtgtta aagtattctt tattggaata
     8761 agtaggagga atatgatgga tatccataaa ttcgaagttt ttcttgattt agccgaaaca
     8821 atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc aaggaaatat ttctaaacag
     8881 attctttcgt tagaaacaga attagacgtc aaattattcg aacgcgccca tagaaaaatc
     8941 gaattgaccg aagctggaag cttattgctc ccatatgtaa aaaatgtcgt agaacaatac
     9001 catgctatgc aagaagtgct tgaaacctat acgaaagaca acaatctttc attaaatatc
     9061 ttaacgatac cgacaatgat caattataaa ggtttttcca aaatcaccga gtttttgaaa
     9121 cgccatcctg aatttacagt ccagttgaaa gaagtggaaa gtgtcgaatt aacttacaat
     9181 gacgccaatg aacatagtga cacgatccac tttgctagaa atttccaggc cccaccaagt
     9241 tcgattgagt ggttgcctac tgaaacagac gattttgtag cagttctacc taaaaatcat
     9301 cctttagcta gtaaaaagaa attggatcta agcgaactga aaaaggagaa ctttttgctc
     9361 cttggcccca ctacgaactt gtatcaacct gtactagatc tgtgtcgcga agctggattt
     9421 gaaccaaaaa tctcttataa aggagcaaga atcgacttga tcatcaatat gattgcaaat
     9481 gacatgggga ttgctattgt tatggaaaaa acagtcaaga atctattaaa agaaaatact
     9541 gtgattctcc ctatttctcc aacaacagag agttatcttg catttactcg taaagtcggt
     9601 gagcattctt tggcttcaga tacattttgg gagtatttaa atcaagaaga aaactaagcc
     9661 ctacattttc tgtccatcta tccacccaaa aaaagagagc gacgcatgac tttcgctctc
     9721 tttcttgcat catgtccttg tttcaatgac acctctaaca tttctcgtag ttcaataaga
     9781 aagcagcgat ttttatgcca aaagaaccgt aagaacattt ccctaatcac aaaaaaacga
     9841 ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt tttattcttt ttcctattgt
     9901 ttacttaacc gtatcattgc tgtcgttcct actccttcga tactgctata atcaatacgt
     9961 ccatcatgca gttctagaat ttttttaacc agtgaaagtc ctagcccatt cccagaaatt
    10021 tgacgacttg tatctccttg ataaaatttt tcaaacattc gatctctggt ctcttcattc
    10081 attcctacac ctgaatcggt cacttccagt ccaatctctg tggctgtctc aaatagtttg
    10141 atatgaatct gtccattgat ttgattgtac tttatggcgt tatccatgat attcagccac
    10201 acttggtaca aaaactcttc attgcctgtg taatttactg cagcaagttc gatatccaat
    10261 tctagctgtt ctttctccca tttaggctgc aaaaaaacga ttacttctct gatttgttca
    10321 tctaatcgat actctttctt ttccagttgg atacgctggt tttccagttt gttcaatttt
    10381 aagatgttct ctgttaattg tgaaagctgc gtgatacttt cgataatccg atataaaaat
    10441 atttggcgtt cttcgtcaga gagatttggg gcttgtaaca attgaacata tccttggatt
    10501 gttgctagtg gtgttttgaa ttcatgggat acactggaca caaagtcatt cctcagtgtc
    10561 tcgatactat tgagttcttg gaccataacg ttgaaatctt tatacagttg ctgtacttct
    10621 gctactttct gctgttcatc cattcgaatc gaaaaatctc ccgttgctac cagactcata
    10681 tttgtcccta gcgcactgat tggatgcagg atacgccgcc caacaaaaat cgatattcct
    10741 gtacccacga ccatactgaa ccccgcaaag acgaataacg ggtataacgt tggttttcca
    10801 gccgattcag caggcgagat agaatgtcga gacaataaga aaataaaacc gagaaaacac
    10861 agcagtgtca ctaagatcga aatgaagaca atactagcaa aatagatcca taattgtgaa
    10921 aatagctttt ttttcatctt taaataaccg ccttatagcc tagtccccgt actgtgatga
    10981 tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg tttgatatgt acatcgatcg
    11041 ttcgttcttc tgtattcgta tctaatcccc aaatatcatc catcaactgt tggcgagtga
    11101 agatcttgtt gggataagac agtaatttat acagaagcag aaattctttt tgaggcagaa
    11161 tcaacgaatg ctgatcttgt aacactgtat aagcatctag ctctaatttt gtttgtccga
    11221 ttatcaagct actgctgtaa ttgatctttg aacgtcgaag caatgcttcc acacgtagga
    11281 tcatctcatt gacatcgatg ggttttgtca tataatcatc tgctcctaat ttgaacccgc
    11341 gtttcttatc ctcaaaatcg gcctttgccg taatcattag aacaggtgtg tcaaatctgg
    11401 tgctacgtaa tgagtctagc aagtcatagc catccatttt cggcatcatg atatctgtaa
    11461 tgattaattc tatacgtgtc gcttctagtt ttttcagcgc atcctctcca ttcaatgcgg
    11521 tgatgactga aaagcccgct cgttccaaca cagaacggta aagcaactgt aattgcggat
    11581 catcttctac tactaaaata cgattcattg atctccctct gttttttgaa aaatttctag
    11641 attttttctt gtagttcatc ttcagttaac atttatttcg tatactacga tcattgttct
    11701 gatatactta ttagtatacc gaatatttca atacgggaga agggtgatta tgcttcaatt
    11761 aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc accaaagcat tggatggtgt
    11821 gtctgtcgct ttcagacaaa aagaattcgt agcgatttta ggtcctagtg gttctggtaa
    11881 aacgactatg ctgaatgtga ttggtggact agataattat gattccggtg atatggtaat
    11941 caacggaaag tctactaaag actttaagga cagtgactgg gatgcctacc gaaacaactc
    12001 tatcggtttc atttttcaaa gttacaactt gattggtcat ctaggcatta ttgaaaacgt
    12061 tgaacttggg atgacattaa gtggtgtttc taaagacgaa aaacgaaaac gggcagaaga
    12121 tgcattgcac cgagtcggct tgacagatca tatgcataaa aagccaaatc agttatctgg
    12181 tggacaaatg cagcgagtgg ccatcgctcg agcactagcc aatgacccag atatcctttt
    12241 gtgcgatgaa ccaaccggag cgttagatac agaaacaagt atccagatca tggaattgat
    12301 ccaagagtta tcgaaagaaa aattagtgat catggtcaca cataatcctg aactggccca
    12361 tcaatacgca gatcggatca ttgaattttc tgatggtaag atcttaacag attcccatcc
    12421 tcacatcgaa cgaccaaaag atgatcaatt taacttacgc agaacaaaga tgagtttttg
    12481 gacagcgtta aagctttcct tcaacaatat ccgaacaaaa aaaggacgta ctttcttgac
    12541 ctcctttgct tccagtattg ggatcatcgg aatcgcgatc gttctgtccc tttcttctgg
    12601 attccaaaaa caaatcgata acacgcaagc tgaaacgatg gcgaaatttc cgattactat
    12661 ttctaaggta acgactaacc agactcgtga cgatgccgga ttgggtgcaa gcaaagcgga
    12721 ttatccagac agcaaaacga ttactgcaaa agtcagcgaa gaagataaag cacagcacac
    12781 gaataagatt gatcaaacat acgtcgatta cgtaacagac atcgatccta acttaagtaa
    12841 taatatcgga tttacccgta ctacaggaat caatctttta cgtgatgtaa atggcaaagt
    12901 acagcctgtc agcttctcga atcaaaatcc tgatacggaa tctctttctt tatctagtac
    12961 gatgtcagca atgacaggtg tgggagtgtc aagtttccct acacagcttg acactagcaa
    13021 agaaaacttt ttaaaagaca attattcttt acttgccgga agttatccag cctcagcaac
    13081 agatgtcgtg ctgatcgtag acggcaataa taatactaat atcaacgcat tgaagaatct
    13141 tggatttgat gtgaaagaag acgaaaaact tgattttgat gatatcgttg gtacaacatt
    13201 caaattagta aacaacaata cttactatac aaaacttcca actgggaatt ttatccctaa
    13261 tacagattat gatgcgatgt atcagaatgc ttctgatgaa ttgaagatct cggggatcct
    13321 acgcgtgaaa tcttcttcta caatgaatct cttatctcca ggaatcgctt atagcgatca
    13381 attaacgact caaatcgtca acgaaaacaa agaatcagaa atcgtcaaag ctcaaagaga
    13441 cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa aatgccaaac aaacattgct
    13501 tagttattta ggtggagaca gcttgccttc aagtatcatg atttatccga acaattttga
    13561 agacaaagaa aagatcttgg attatctaga tgattacaat aaaggcaaat cagatgaaga
    13621 taaaatcatt tatactgatt tagctggaac gatgactgaa ctgactggcg gattgatgga
    13681 tgctatcact tatgtcctta ttgcttttgc agggatctct ttagtaacaa gtatgatcat
    13741 gatcagtatc attacttata catctgtgat tgaacggaca aaagaaatcg gtgtactaaa
    13801 agctttaggt gcacgtaaaa aagacatcac tcgcgtattt gacgcagaaa cgtgtatttt
    13861 aggtatttct tctgggatat taggtgtgtt tattgcatgg ctagctacgt tccctatcaa
    13921 tagcatctta tacaacatga cggatctaaa aaatgttgct caactaaatc ctgtccatgc
    13981 gattattcta gtgatcgtct ctactatatt gacgatgctt ggcggtcatc ttcctgcacg
    14041 aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca gaataaggtt gtgacaacca
    14101 catttagatt tgagtcataa gagcgctgaa atagtagaaa acagcaagaa acgaggaagt
    14161 aatatgttcc ttgtttcgcg ctgttttttt taattatttt ttataattct ggtatacttt
    14221 tctttccttt gtaccacaac tcttctggcg tatacctgtc ttttccattt atgaatactg
    14281 ttccatctgc ttcaatcgtt acttcagtcc caatccacat attacgagaa agtgcgagaa
    14341 gattttttct ttttcctctt cctttttttc caaattgcaa ggcatcggtt atctcttctg
    14401 tccaatgaga tttcacgtat tttacttctc cgttttcaaa tgtaactttc aaaatattcg
    14461 ctatattttc cacttttact gctttaggaa atgactcctt catttttttc actttccttc
    14521 ctccaacact aagtatgcgc tttcttttag tgataagtat atcatagttt attctattat
    14581 caaaaaaacg catccattcc attgtagaat actttaccga acaagttgaa ttgttttcct
    14641 ctttttcacc ttttactata gataggtaaa gacaactaag gaggaatctc tcatgacaga
    14701 tcaaccgtat gatttaagaa aagtcatcga agaactaaaa acacttccag gtcaatatca
    14761 cgaaacagat attgaaatcg atcctgaagc agaattatct ggtgtttacc gttatatcgg
    14821 tgctggcggt acagtaaaac gtccgactca agaaggacca gctatgactt tcaacaatat
    14881 caaaggtttc cccggcacac gtgtgaacat cggaacaatg gctagtcgta aacgtgtagg
    14941 tgaaattttg caccatgatt atcgaacact tggtcgtttc ttgaaagacg ctgtagaaaa
    15001 tccaatcaaa cctgtgaaag tagacaaagc agacgctcca gcacaagaag tcgttcatct
    15061 agctaccgat gcagacttcg atattcgcaa cattttgcct gcacctacaa atactcctga
    15121 agatgctggt ccatacatca caatgggtgt agtcttagga tcagatccag aaaaaacaat
    15181 gagcgatgta acgatccatc gtatggttct agaagataaa gatacgatcg gtatgtacat
    15241 catgcctggc ggacgccaca ttggtcactt ccaaaaacaa ttcgaagcct tgaataaacc
    15301 aatgcctatc actatcaata ttggtttaga tccagcaatt gccattggca cgactttcga
    15361 accacctacc actccacttg gctacaatga actatggtgt gcaggagctt tgcgtaacga
    15421 acctattcaa atcgttgatg gtatagcagt cgatgaagtg ggtattgctc gtgctgaatt
    15481 catcattgaa gcagaaatca tgccaaatga aacaatgcaa gaagatatca acacgaactc
    15541 aggaaaagca atgccagaat ttcctggcta tactggggat gctaatccag ctgtacaagt
    15601 agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca atcatgcaaa caacgattgg
    15661 tcccagtgaa gaacacgtat ctatggccgg tattcctaca gaagcaagta ttcttgcctt
    15721 ggtcgataaa gctattccag gaaaagtagt aaatgtctac aatcctcctg caggtggtgg
    15781 taaattgatg accatcatgc aaatccgtaa agaaaacgaa gcggatgaag gtattcagcg
    15841 tcaagcagct attcttgctt tatcagcctt caaagaattg aagacagtca ttttagtcga
    15901 tgaagatgtt gatattttcg acatgaacga tgtagtttgg actttcaaca ctcgtttcca
    15961 aggagataaa gatatcatcg tgatcccagg aatgaggaac catccattgg acccatcaga
    16021 attaccagaa tataatcctg gctctattcg tttccgtggg atgagtgcaa aaactatctt
    16081 tgacggtaca gttcctttcg atatgaaaga acattttgta cgtgcaaaat tccaagaagt
    16141 agaagattgg gaaaaatatt tgaaataatg aatgatctgc aactgttttg acatcataaa
    16201 aaggagtgcg gaaaatggaa caattggcac aacggaagaa aaaaatcgtg attggtgtaa
    16261 gtggcgcttc aggcaccatt tatgccatca acctagtgaa gaaactaaaa gaatatccga
    16321 tgatagaaac acacggaatc atcagtccat gggcaaaaca gaacttaaag ctggaatccg
    16381 acttatcact aactgaattc gaaagcctgt ttgattacca ttattcaaac aaagatttag
    16441 gtgctgctgt cgccagcgga tcatttttga ccgatggaat gatcatcgta ccggcaagta
    16501 tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa cctgattgca cgtgctgctg
    16561 atgtaacaat gaaggagcaa cgaaagttag tcattgttcc tcgtgaaacg ccattaaatg
    16621 ccattcactt ggaaaacttg actaaattag cacgactagg tgtgcaaatc ataccaccaa
    16681 ttcctgcttt ttataatcag cctcaaacga tccaagatct tgttaatcac catacaatga
    16741 aacttttgga tcaatttgga attgtaaatg agcattcaaa acgttggcag ggtgattaat
    16801 atggaaaaag aaacgatagt tgaagtaacc aaactaggtt tcacgatcca agctgctgtt
    16861 aaagtaatcg gtaaagactt attgatcact ttaacaggcg gagatacacc acatatcgga
    16921 acagtcacta cattttctcg tgatacagag atgcaaactg tacgtttccc tagccacgat
    16981 ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag ctgtgatcat caaaactgcc
    17041 gttccgggaa gttgtacgat cacttccggt atccatgttg accatatctc caatgcacaa
    17101 atcaacgcct cttttccgat gtcagaagag ttaggttaca agattttaga ttggttggaa
    17161 aaaatagatt tccatatcaa agaaccagtt tattataaag atggtgaaaa aacattatga
    17221 aagaagaggt tgtgaaatca gcgttatgac ctgagtatga gagaaaaaaa tggaaaaata
    17281 gcttctcata ttttgccgtt tttgagctaa taccgaggga cagctgattg aacgccgttt
    17341 atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta agaaaaattt tgtaaacatg
    17401 ggtttccaca tttttgcata aatttgactt actaccgaga aactggcttt tgaacaccgt
    17461 ttatcaagag gttgtgacac gtcttttgtc acaacctctt ttatttttgc ttcactgtaa
    17521 ctgctacaat atcaccgatt tcttttccga tgtttttacg aatatctttt cgtagtccaa
    17581 ggatgtagca gactgaacca tctggatttt tcacgcccat attcacgata ctgccttcat
    17641 acggcatatt gtcaaaagtt gcttggactt taactcttcc agcaccgaat tcttttctta
    17701 tatcatacgg aaaaataata tatgccccgc ccttgtcgac ttcggaagca tagatttttg
    17761 attcaaaaga ataaatttcc ctcattttat ttctcctatt ttttacatct agtatagcat
    17821 agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca gttccttttt tcgaataaac
    17881 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcaacaaaca tgagaagctg
    17941 ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac ctcttcctta
    18001 aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa aatatggagt
    18061 gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca caacctccta
    18121 catggtgttc aaaatattat tcgccttttg cttctttcac taattttgcg aatgcttgtt
    18181 gttggaattc agcagctaag gctttcgtac gttgttcttg ctctgctttg ctcatttttg
    18241 gattttcagt cattgctttt accataccgt cttgaacata agcttttgct tcactttcca
    18301 tcgtactttg ctgtttcttc actaattcag ctaacttggc tgcttggtcc gtacttaatt
    18361 ctacccatgt ctctgggatt ttgaatgtat tcactcggct gtcatattcg tgattgttat
    18421 cagcaatatt gaaccagaat ttatacatgt cgtttttgta ttcccaataa gtagttttcg
    18481 tcactttttc agcatcgccg tctgtctttt ctactttatt tgtttcgtta tccgtaccag
    18541 ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag atacactttt tcttttccat
    18601 tccctaaagc tttatacaaa agcatatctg ctccttggga atcagcactg gaaacaattt
    18661 tttctgtttt tgtttccgtt actttttcca ttccaaagtg attggacaaa ttagcgacga
    18721 tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa agataaaaag gtttgccatg
    18781 tttttttagc gaacacgttc gttaaagcaa aagcaagcaa gctgacaatc aaaactccaa
    18841 taatcatgca ttatcccccg ctttcattag aacaggttct tttttcttca ataaaaaggc
    18901 aataataaag ccaagaatcc caaagatgat cgctacgata aacgcggcat ggtaacctga
    18961 cagtgtggca ttgattgcct gatctttata agctaatggc gtattatcca atacagattt
    19021 gcttgggata tttgcttttg tcacattcgt caacacactg atcaaaatag ctgtaccaat
    19081 cgagcttgcg atttgacgga atgtattatt taccgctgtc ccatgactga tcaagttatt
    19141 tggcaatgca ttcataccag aagtcgttac cggcatcatg accatggaga tcccaaacat
    19201 acggatcgca taaaggatca cgatatataa aactggagtc gtttttgtca agaaagcaaa
    19261 tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg gctaaacgtt tcgcgccgta
    19321 tttatcgaaa atcgcacccg tcacaggcat catagccccc atgatcaatg cacccggcaa
    19381 caacatcaag ccagaatgga atgctgattc tccacgaata ttttgaatat acaatggtac
    19441 gaccatttct gcccctacca tcgccatatt tgttacacca gctaaaacag ctgcaatcgt
    19501 gaatgtagat gatttgaata cacgaagttc caagaatgga tgttccaaat gtaattgacg
    19561 ccatgagaac aaggcgatga aaatcacacc aacaactaag aaaccaatta ctttcaagct
    19621 tccccagccg tcatctccta cactagaaaa tccgtaaagt aaactaccga atccaatcgt
    19681 tgataaaata gcggataaaa tatcaatgct aggatttgac aatggaataa cacttttcat
    19741 taggaagaat gctaaaacca agacaagaac aacgattggc agtaccatac caaacaagtc
    19801 acgccaatgg tagttatcaa tgatataacc agataatgta gggcctaaag caggagcaag
    19861 tccaataacg atccctgaca tccccattgc tgatcctctt ttttctggcg ggaaaataga
    19921 tagcatgata ttttgcaata atggcatcga tactcccacg ccgcaagctt ggatcaagcg
    19981 accgattagt aatgtactaa aattaggtgc tgtaaagcaa gtaatcgttc cgatcaaaaa
    20041 gatcgtcatg gcagaaagat ataaatttct tgaactgaat ttattgatca gccatgctgt
    20101 tattgggatc atgatcccgt tgactagcaa gaaaccagtc gttaaccatt gcacatcaga
    20161 cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct gtcgtcaata aggtctgatt
    20221 caaaacggta caaaatgtac caattaataa gacaacaact aaaagtgtcc gattatatgg
    20281 ttttccgtaa atatctacgg gttggttgtt actcatattt ctccttcttt ctgaattttt
    20341 tctgaaacta ctagaatact ttagtcctaa attcgctatt tgtcaaccta cttgacacat
    20401 aagttaatac ggatataatt aagggaagca agaggacaaa aggagtgaca aaaatggttg
    20461 gggcttccct taaacgtttg ttggaaaatg atcaattggt tgttggcatc agtgaattaa
    20521 gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg ggaacaaaag ggattcattc
    20581 gttcgatcga agctgatgcg aattgtccta gaaaatatcg tttacctaca gtcataaaag
    20641 tagaactgat caaaaaatat ttagatgacg gatttacttt gtcaaaagct gcagaaaagg
    20701 cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt tttttcaaaa gcaatcaaag
    20761 atatcgaatt gatcgacgaa cgctatacga ttttacaaat cggtaacttc aaagaagaga
    20821 atgctaagat gtacatcatt catgacaacg agacagacga attaacttac aagctccttc
    20881 ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga actctaaacg ccactaatct
    20941 cctaagtacc aagcttttta ataagtagat gctacataaa tacttgaaaa aaggcctgtt
    21001 tgaacataag aatatccgaa cggtgatctg cgtttctttc atatagaaag gaattctcca
    21061 tcactgttcg gatatttttt tatttttttc ggttactttc ttctaataca tctgttagcc
    21121 aatttgcata ttctgtttcc cgattgatcg catgcgctaa aatcaggtag tgtccgtaat
    21181 tttcatgttt ttctttttca gtaggaaaga tcgttttttt tcgctctaat agatgaagtc
    21241 gtttttcttc atgaaggtca cgctgctgct gaataatttc tactaatgcc ggatcattca
    21301 cgtctttgac gaaatacaat ttcagaacaa attcgtctcg attaaccggt agttcattgg
    21361 ttggtgtatg aatccactct gccaattgtt ccttcccttc ttctgttaat tcataaactt
    21421 tcttttccaa tttttcacct gtgatctcta cttgatgctg aatgattcct tgctcttcca
    21481 tttttgcaag ttcaggataa atctgactat gttttgcttg ccaaaattct cctatttcat
    21541 ttttgaacga ttgattcaat tcatatcctg tcatcttctt cttacttaat aaccctaaca
    21601 aaatatatcg aagtgtattt tttcttgcca tcagttttcc ctccttgaca aatctttaaa
    21661 tccattctat cataaatgag taatcgaaaa catgtagttt attacatgtt ttaatttata
    21721 aggagatgct taaacatgaa agaatttaaa gaattggaag attttattgg aactcacttt
    21781 atctatacgt atgacaatgg ttgggaatat gaatggtatg ccaaaaatga ccatactgtt
    21841 gattaccgta tccatggcgg aatggtcaaa ggccgttggg taaaagacca agaagctgat
    21901 atcgttaaat tgacggacgg cgtattcaaa atcacttgga cagagccaac agggacagac
    21961 gtggcattgg actttatgcc aaatgaaaag aaacttcacg gaatgatctt tttccctaaa
    22021 tgggttgaag aacatccaga aatcactgtt actttccaaa acgaacatat cgcagaaatg
    22081 gaagcagctc gtgaaaaata tgaaacttat ccaaaattac ttgttcctga atttgctacg
    22141 atcacttata tgggtgatgc aggtatagac aatgaagatg ttattagcga agaaccatac
    22201 gatggaatgc cagatgatat ccgtagcgga aaatacttcg atgaaaatta taaacgagta
    22261 aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct gacctgagta ttagagaaag
    22321 aaacggaaaa atagttcctc atgttttgcc gttttttgag ctaataccga gggtcagctg
    22381 attgaacacc gtttatcaag aggttgtgaa atcagcgtta tgctttgagt aataagaacg
    22441 ctagttagaa aatagctttt catattttct aggctagtgg gcttattacc gagaagcagc
    22501 cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt tttgacctga gtattagaga
    22561 aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    22621 ctgattgaac accgtttatc aaagattgta acaactgcat attcacacag ttaaattagt
    22681 gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc tgggagcatt atttcgttat
    22741 tctgttcatc aaaaatcagc gttttgattc catttcatca caagttcctc ctcatttact
    22801 gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg aaagatagaa atgaatagct
    22861 gattgatttt tttgatagac tgtcaaagtc agtgttttat tggtttcttt tatttttttc
    22921 attagttgcg taccgattct ttgttgccgg tattcccttt tcacaaagat tcctgcgata
    22981 aactgatcaa tcaccccagc aaaaccaata atttcttgat ctatttctgc taagtaaatc
    23041 actgcatctg gtaaagcttt cctaacagat tctacttgat tctcccaata agttgatgga
    23101 atgaattcat gagaatccaa gttagcatgc agccaaatcg atagaacaga ttccagttct
    23161 ttggcagaca attttgttgt tgtgcggatc attttttctc tccttactta ttcattgatt
    23221 ttaaaataaa aaccattttt ttgaagctct tttacaagtt ttcgcccctc aaaaaagtat
    23281 atcttctctt gttttacttt tctaatacca aaagaaacag caactttagg atcatcagct
    23341 gaaacgattc gataagttcc accaggcaat tgatccagct tgactttccc atgatggtcg
    23401 ctttgtactg aaaacggcac accatttcta gtaagtgttt ttttcttatg tttcaaaatg
    23461 aatgaactac catttaccgg tttccctgac gaatcactaa taataaatga aaaatcaagc
    23521 acttttttac ttaatctata ctgatagatt agtcgtccga taaacactaa cagtatgact
    23581 acagctatta tcgttcctgc aagaataaac cattgccgca tggttctttc ctgatttcct
    23641 tttccttctt ctttttttac cgattcgtta taaggaatcc gatgtcctgt cactagcaaa
    23701 cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg taactaagtc ttctccttct
    23761 gaaatcgtta gaacagacgt gtcatctggc agtaccactt gtatattttt aacttgataa
    23821 gccaatttct cccccaaaac agtcaaaata aacgtgtctc cgttttctaa acgatctaat
    23881 tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag agatcactga atgcgtcgaa
    23941 tttccaccaa gcgggaaaga ggttccttgc aagacgcctg ctccattttc aaggatccta
    24001 ccattgagcc gatcataaag tgaaatattt aattggatct taggtatatt gatacttccg
    24061 atcagccatt cctctaattg gctggatgtt gtactttcat ttctgttctt ttcattgaaa
    24121 ggatcagtac caggattgaa tccttggtca gttaatcgtt gttttttctc ctccattttc
    24181 tttcgctgca cttcaatttc agctgcggtc cgtttttgta cttgttcgat acgtttttga
    24241 tcaataaaac tatttaaaga atccacataa aatggatata aagcgatcag taaccctacg
    24301 acgatacata ttaataaaaa caaacgaaac aaaagtcgac gagactgttt ttgcttactc
    24361 tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt tcttttatta tatgggttca
    24421 ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa agctgtcctg catcaagtaa
    24481 taagtacagc ccaaaaaaat agctactcat attttttgtt gggttgggct tattaccgca
    24541 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aaactgcgtt atgctttgag
    24601 taataagagc gctagtcaga aaacagcttc tcatattttc taggctagtg agcttattac
    24661 cgagaagcag ctgattgaac accgtttacc gagaggtcat gaaaaagcta ctttgtttcg
    24721 agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa caaaacttat gtcgcatcct
    24781 ctctccttag aactttcact tatgtttttc ttgtaaaatt cataaatatt ttcatgaatt
    24841 ttctaatttc ttgcctacct ctcgcatagg atatatgatg aatttatcta aagtttgcaa
    24901 aggagttcga caattgaaaa aatatggccc atttttagca atttttttca ttgctttggc
    24961 atttctcatg cctcatttat tcacacataa actaattttg ggtgctgatg ccccattcca
    25021 ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat ggggatttcc attattttcc
    25081 ttccctttat ggattccagc aaagtggcag gatcgtgaat gccgtttacg gcccattttt
    25141 cgcttattta caaggattga tcgtgttgct tgcaggtagc tggtttcatt atcagattct
    25201 ttcaaatttc attctttatc ttatttcagg attttctatg ctgattttac tgagatatgt
    25261 caatgttcga aattgggtaa gtgtaccagt agccgtttta ttcatgagta cgtattctat
    25321 tcaatactgg tctatcaacc aaggattcac tagctggggg acttgttttt atcctttatg
    25381 tatgatccct attgctgatt tcgtccttca taaaaagttc cctgttatca aagtcgctgt
    25441 ctcagtggga cttatgacac agatccattt actgtcttcg atcatgttag ctttgatgta
    25501 tatccctttt tatctttatt acttttttaa ccagactgaa aaaggaaaag cactgcttca
    25561 actgattaag tcggtcttgc tttattttgt tctgacagca aatatttggg caggattagc
    25621 tattgtttac agtgggaata agattcttcc tccctttgtt aacagagaaa tgagtcaaaa
    25681 ggcgatagat ctggatggca gttattggct ccattaccct aaggcctttc ctatattgat
    25741 attatcaggt tttttgcttt ttattttctt tcaacaaaag aacaccttat tccaaaaaat
    25801 cgtgttgtac acaagtcttg ttttcttttt tctttctact agcttgattc catggacgac
    25861 attgatcgaa aaaaatgtgc cttttattag tttgatccag tttccatttc gatttttcgt
    25921 gccatttacg ctattgcttc tactttacct tgccctttca ctgaaccagt ggtctgaaca
    25981 aaagtggttc cgtcttttaa gtatatttgg cattcttatt ttttcagcac agacgatcca
    26041 gaacttatac cagcatctgg aaaagtggga aaatgaaaca tttgtcagtc gacatactta
    26101 tctattcgat acgccggaag aagcaaggaa aacattcttc agcaaagata tgtctaccag
    26161 cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca atctataagg agacaaaaga
    26221 caataaatac gaccggtata acgaagaaat actagaaaac gaaacaactt ttataaaaac
    26281 acatccaggc ggtcttttga caatcaagtg gaaaaacact ggggacaaaa ctgttcatat
    26341 ccctgttatc gtctacgatc ggactgtgct acagcaaaat ggaaaaacat tgactgatta
    26401 tgaagtaaca gatatcggga ccccaatcgt gaagcagaaa aaaggaatca acgagctcac
    26461 gcttcattat caaacgccaa tctatttcta tttcattctt tctcttactt tgattggctg
    26521 gtttaccttg ctatgtttat ttatttatca tagatacaag cttttacgtg cttaggcaaa
    26581 agggataaat acatccgatc aaaaaactct ccaaacggta taatcgtttg gagagtttta
    26641 ttttaatcag tttttttatg ttatatgtta tattttttat tcgttcacat ctaattcgat
    26701 ttccaccccg tagtgatcac tgatgattgg actatcgatc ccatcaaaaa tccgttgata
    26761 ttttttaacc tgcattcctt cagggacgaa aataaaatct atacgcaatt tttctgtatt
    26821 ttgttcccaa ccatctattt tcttttccac agtagctgct tttccttttt cattcgcgac
    26881 aacatacgaa tcttgaagaa gaaggcaact ttctcttaca gtataataac cttgagagtc
    26941 tactggatta ttgaaatcac ctaggaaaaa aagtggtacg cgagtttcca aaaagtagtt
    27001 ttccaagttt ttccactcaa aataaaagcc acttttttta tctgtccacc aagaaaagtg
    27061 gcagctcgag acaaaaatgt cctgatcatc tatctttgtt ttcccaatca agatcttgcg
    27121 tgtctgataa tcttctttct tttgagattg ggagacggta aggacctgac tttccaatgt
    27181 ttttttcgta agcagtgcat ttccttcttc atatttatca taaccgatgt ggctcatttc
    27241 ccaactccaa aaatactctt ttccgtgttc ctttaaaagc tgaactaaac ggtaggcaaa
    27301 attatcttta cggataggta cttgattctt tacctgacag aacttcatta gcttcgcagg
    27361 ttctacttct tcagattcca gtaattggtt gatttcttgt aaagcaatca cttcataatc
    27421 ttctaacagg atcttatctg ctaattcttg caattttttc tctggttctg gctcaagcca
    27481 gctatgcgtg ttgagtgtca tgaatttcat tggttttctc cttgtcttca tttcgaaaca
    27541 aaagggttgt gacaaaattt ttgtcacaac ctctccagca aatagtgttc tcctgctaac
    27601 tcctcagtaa taagctcaat ggaccaggag ctattttttg tttggctgtt tttatcactt
    27661 ggtgcagggc agctttttca cgacctctcc actaaacagt attcaaactg ctgcttctcg
    27721 gtaataagcc caaccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatga
    27781 cttgatgcag gacagctttt tcacaacctc tccttacatt ttaatggagc cgatcacttt
    27841 accagctgtc actgtaccta attgcttgat cactacttct tctactttat cgtttgtaaa
    27901 tacgatcatg atcgttggat ctttttcttc gttttggatc acatcaagat ccatctcagc
    27961 taatttcgtt cctgttccta cttcttggcc ttctttgaca gcaatctcaa aagctggctg
    28021 attcatttgg actgtatcga ttcccatatg gatcaaaact tcgataccat ctttcgtttc
    28081 cagcccaatt gcatgtttgc tagggaaaat acttgtcact tttccagcga tcggcgcaac
    28141 aacttctctt gatgttggaa taacagcaaa gccgtcaccc atcatttttt gtgagaatac
    28201 cggatctttt acttcagata aaggaatcac ttctccagaa gctgcggaaa ctagttcttg
    28261 ttctatacct aagtattgct tactacctgc ttgtccattt cctgtagaaa ccatttctgg
    28321 ctctggaatc tctgctcctg attgcagcag atcttcgata tctgatttca agacatctgc
    28381 ttttggtcca taaactgctt ggactccatt gtctttgacg atcaatccca tcgcccctgc
    28441 tcgtttccag gcttcttcag tccctacttt ttctcgatca tttacactta cgcgtaaccg
    28501 agtcatacaa gcatcgacat ctttgatatt ttcttttcct cccagcaaat gaatgatctg
    28561 gaccacttgc tgatctgctg ttcctgttcc gctgttagat acaacggttt cttcactgtc
    28621 attgtcgtaa tttccgttac gtcccggtgt agcaaagttg aattttttga tcaagaaatt
    28681 tgctaagaaa tacgtaacaa caccaaaagc gatcacacac aacacgaagt tgataagatc
    28741 tccacctaga cccgctttga tagctaacgg tgtccgagtc agcaattcga tattcccaaa
    28801 tgaatggacc cgcaatggca agatatctgc catcgcaaat gcagcccctt ggatcactga
    28861 ataaatgaca tataatggca cagcagcaaa cataaacatg aactccaatg gttctgttac
    28921 acctgttaga aagacagcca acgctgctga aaaatacatt gatttatatc ttgctttctt
    28981 atcaggatcg acattacgat acatcgcaaa agccattccc atcaagatac ctgaagaacc
    29041 aatcatttgt cctactttga aacgagccgg cgtccagtta tgcaacacaa actcatattg
    29101 cgacatatcc cctgcacctt taagattcac taaatcagtg gcccaagcca accataacgg
    29161 atcttgccca aatacttgtg tccccgcctg tgcgcctgat aaaatctcat acgatccgcc
    29221 aagctgtgta tagttgatag gaatcgtcag catatgatgc aatccaaatg gcaatagtaa
    29281 acgttctaat gttccaaata aaaacggtgc tagaatcggt gcgctttctt gtgattctgc
    29341 aatccataaa ccaaaattat tgattccagc ctgtacgttt ggccacacca cagcaagaat
    29401 caatgccacg attgttgacc ataagataac aacaaacgga acaaagcgct ttccattgaa
    29461 aaatgacaaa gcatccggca attttctata attatagtac ttattataag ccattgcccc
    29521 gacaaatcca gcgatgatcc ctacgaatac tcccatattc aaagctggtg cttccaatac
    29581 acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac agagtgtggg taaatgcatc
    29641 ttcattcgca agcatgtcac ttgtgacacc aaagatagcc ccagtgatcc gattgattaa
    29701 aataaatgag atcccagctg caaatgctcc gcctgctctt tcttttgccc aacttcctcc
    29761 tattgctaaa gcaaataaaa tatgaagatt accaataatt gcccaaccaa tattttcaat
    29821 tactccgccc gttgtgacaa gcggcgtcca atcaggattg atcaagggaa ttgatttccc
    29881 tatactgatc atcaacccag ctgccggcat aactgctacc acaaccatta aagcctttcc
    29941 aaatttttgc caaaactcaa aactaaatat ctttttcatt ccgtttagct cctttacgca
    30001 atcggtttcg ttaaacgtat tatagcgtgc gttttctttt ttgtaaatgc tttatttttt
    30061 atttttctgt ctataccatt ctataaaacc tataatacgt gtgttccctt ctataatttt
    30121 ctcctaaaaa tttatttttt taagcttttt tctatgaaat attttctttt gtctattgac
    30181 ttgtgcaatc ggttgcgttt attatgaacg tataaaacag ctcattagga ggagaaaaat
    30241 gaaacagatc aaacgactat ttgctattga tccatggaaa atcagcacga cacaattaga
    30301 taaaaaaaat cttcgactgc aagaatcttt aacaagtatt ggaaacggtt atatggggat
    30361 gcgcgggaac tttgaagaaa cttactcagg ggaccaccat caaggtactt atttggcggg
    30421 tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa aatggttatc cagaatactt
    30481 tggaaaagtg atcaacgcct tgaactttat cgcgatagat atttcgatca acgatcagct
    30541 gatcgacttg gcttctttgg aaccagaaga cttttactgg gaattagata tgaaaaatgg
    30601 tgtattatct cgtacattca caatcactac ttctactaat aaagtacgca tttccttcga
    30661 acgttttctg agcattgtca aaaaagaatc agcttatatc tatttaacta tagaaatgct
    30721 tgaaggtagc ggaacagtca aactcgtatc aaaattggat ggaaatgttc aaaacgaaga
    30781 cagcaactat gatgaacatt tttggaatga gattgatcgt ggaactatag aagaaatcgg
    30841 ctatttgacg actaaaacta ttccaaacaa ctttgggatt gaacaattta ctgttactgc
    30901 agcaatgcgc catttcgtta atggaaaatc agtcatccct gactatcacg aagatacttt
    30961 agcattacat ggttgcttgt ctgcagaatt atctcaaggt gagacatggt cagtggaaaa
    31021 agaaattatc gttcttacta gtagagatat tccagaagcg cagcaagcag aaaaagctgt
    31081 ctcagaatta acgaaccaga caaacaatta tatccaagca aaagaagagc aaacagcggc
    31141 ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa ggagacgaag aagcacaaca
    31201 aggtattcga ttcaatttgt tccaactatt ctctacttac tacggcgaag atgaacgatt
    31261 gaatatcggc cccaaaggat tcacaggaga aaaatacggc ggagccactt actgggatac
    31321 agaagcgtat gctgtccctc tttatcttgc tttagccaaa cctgaagtaa ctaagaatct
    31381 gctgaaatat cggcataatc aactgccgca agcaatccac aacgctcagc aacaaggatt
    31441 aaaaggcgcg ctttatccta tggtcacctt cactggtgtc gaatgccata acgagtggga
    31501 aatcacattc gaggagattc accgaaatgg cgcgattgcc tatgcaatct acaattatac
    31561 gaattacacc ggcgatacgg aatatttgaa aaatgaagga ctggaagttt tagcagaaat
    31621 cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga gcaaataaat acatgatcca
    31681 cggtgtgacc ggcccaaacg aatatgagaa caatatcaat aacaattggt atacaaatac
    31741 gatcgcagct tgggtcttgc gttacacgcg agaaaattat ttaacttatc aaaaagaagc
    31801 tgctatcact atcagcgaag aagaactagc caaatggaaa gatatcgcag agaacatgta
    31861 cttcccagtt gacgaagaat taggaatctt tgtccagcat gacacattct tagataaaga
    31921 tctcatgcct gtatccgata ttcccacaag cgatcttcct ctgaatcagc attggtcttg
    31981 ggacaaaatc cttagatctt gcttcatcaa gcaggcagat gtactacagg ggatttattt
    32041 cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac tttgatttct atgaaccaat
    32101 gactgtccat gagtcttccc tttcgccaag tatccattcg attttagcag cagaattagg
    32161 aatggaaagc aaagctgtag aaatgtacca gcgaacagct cgcttggatt tggataatta
    32221 caataacgac acagaagacg gcttgcacat cacttccatg actggtagct ggttgtcgat
    32281 tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag ttgagattcg caccatttct
    32341 gccaaaaact tggaaagctt attctttcca tatcaattat cgcggacgtt tattgttcat
    32401 ctcagtcaca gatgaacaag ttactttcga gttattagaa ggttttcctc tagaaattta
    32461 cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa ataacgctaa gaaaggagga
    32521 agcaaatgtt taaaggggta ttgtttgatt tagacggtgt cattaccgat actgctgaat
    32581 tccattatcg tgcttggcga aagcttggtc aggaaatcgg cattacgatc gaccacacat
    32641 tcaacgaaca gctaaaaggc gtcagccgag aagactcact ctctttgctt ttagcttatg
    32701 gcggaaaaga acactctttt tcaaaggaag aatttgcaga attagcgaaa agaaaaaacg
    32761 attattattt agagatgatc cagacgatcg aaccaaaaga tgtcttcccc ggggtcgtgc
    32821 ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc tcttgcttct gcaagtaaaa
    32881 acgggccatt cctacttgaa aaaatgggtc ttacacctta ctttgatgct attgccgacc
    32941 cggcagaagc aacaaacgga aaacctgcgc cagatatctt cctccttgca gcaaaagctg
    33001 tcggactaaa agcagaagaa tgtatcggga tcgaagacgc gcaagccggt atccaagcca
    33061 tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc tgaagacctt ggagaggaga
    33121 tcccgcttgt ccctgatacg acttttctta cgatcgatta cttgaaagaa aagtgtcatg
    33181 accatggata atccagagat ctccgtcgta ccagttccag agactgaaat aaagaaaatc
    33241 acattgaaga acgactactt agaagtcgtt cttctcaatt acggtgcccg cctgcaccaa
    33301 atcttcgcac cagacaaaga aggaaaatca gaaaatgttt tattgtctta cgatcgtttc
    33361 gaagatgttc taacagacaa aagctttttt ggagcaactg tcggacctgt agccggaagg
    33421 atccgggatg cttcttgggg tgagcataga ttggagaaaa actgcggatc acaccatatc
    33481 cacggtggca cgaaaggctg gtctttccaa ttttgggaag tagaagtttt caaaactcca
    33541 tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt ctacctatcc aggaccaatc
    33601 actgctacga tcacttaccg tttaacaaaa aacgaattag agatgacaac ctctgctagt
    33661 agccttgttg aaaccatctg caatccgacc aaccatgcct acttcaacct ttcaggaaat
    33721 ggaacaagag acatctatga acatcagtta acggtctttt tagatggtgt gcttgaatta
    33781 gatcaagaaa aactgcctac tggaaattgg acaaaaaaag aaaaactgcc aatcgacttt
    33841 agaaaatccc caacactgca agagatcctt gcatgttatc cagatggact agatgatgta
    33901 tttcttttgc atcatcctag gctttccaaa acttccttac aattattcga gaagcattct
    33961 ggtcgccaaa tgactatagc aacttctaat aaaagtatgg tattattttc tacgactgga
    34021 ttcgaagctg atttctctgt caatggaaaa cagatgcatt caaactacgg attagcaatc
    34081 gaaccgcaag aaatccctga tatcgttcat ttccccaaat ttggatcgat caacctccat
    34141 cctggtcaag aacggatcag tcagacgatt tatcggttct ctgcacaata acaagagaaa
    34201 ggtgttttta tcatggctat taccgtaaaa gatgtggcga aaaaagccgg tgttgccacc
    34261 tcgaccgtct ctcgtgtcat caacgatcat cctagtattt ccgagacaac aaagaagaaa
    34321 gtccgaaaaa ttatggatga actaggttat gttcctaaca tgactgcccg caatctagga
    34381 aaacgcattt ccagtgccat cggcgtaatc ttaccccctc ttgattcaaa agagcggatc
    34441 ggtaacccat tttatcttga gatcatggaa gcaatcaacg aagaagcgcg gaattatgat
    34501 atgactacag caatcgctac tgctaaaaat tttgaattgc tgttagagaa cgtccagcgt
    34561 atgcatttac aaaaacaagt agatggtttt attctagtgt attcagatag tcaagatcct
    34621 gtcattgatt atttatacca aaaccacatc cctttcactt tgatcggaca gccttatcat
    34681 catgagaacg aaatcgttta tgtagataat gataaccagc ttctaggtaa acaagcaaca
    34741 gaatttttga tccagaatgg acataagaat atcctgtttg tgacaaacac gactcatgaa
    34801 agtttgtatt tcgaacgata tttcggttat caaaaagcaa tgatgatggc tggtcttcct
    34861 gcgttatcca gtgtagtact agagcatccg gaggattatc tgtcctttga gaaaacgttg
    34921 aaagaaaacg aagccacagc attggtggtc attgacgata tcttcgccct tcgcacgatg
    34981 cagttggcgc aaatgtacgg ctatgtcatt ccagagacac tctccgtcat tagcttcaac
    35041 aactcgatct tctcaacatt aacacaccct taccttacaa gtatcgatat cgatatctcc
    35101 gaattaggac gtgtcgctat gcaaaagctg cgcgaattga tccatgaaga attgagcagt
    35161 ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag agacagtcgt tcctttagaa
    35221 aacccatact aaatagctga ctcactaaat acgctgacca tttatcctat ggtcagcttt
    35281 ttatgtaaaa gaagatacta cttctaacac attgtttatc tgtaaaaaag agcgtgggac
    35341 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    35401 aactccttcg gaaataagct gaaattcaca aaaatttaaa aagcaatttt cttgagtttc
    35461 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttccttc cttcaatttt
    35521 ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt aaagacaggt gcaagaatcg
    35581 agaaaaagac gaatggcaag taatgcaacg cgctaacttt catcgttccc ataatgaatg
    35641 tgccgctgac tccccacggt acgagagaat tgacggtcgc acctgaatcc gctagtgttc
    35701 gtgtcaaata ggtttttgat aagccaacgt catcaaaaga acgcttgaat gtttctcctg
    35761 gaagaatgat ggacagatac tgttctccaa cgatcagatt gattccgata cagctcaagg
    35821 cagtaaaccc gactaaacga gaaggtcggt ccatcttttc ttttaattct tgaacgatgg
    35881 tatcaacgat cagatatttg atcagtaacc cacctaatgc caatgcaagt aaaatcaaac
    35941 tagcagaacc taacatactc atgatcccgc ctcgagaaag cagttcatct agctttggat
    36001 caggtgtatt tgcgacataa ccattcatca agatgtctgc tgcttttccg accgtcaaac
    36061 ttttctcatg gataaaagaa agaacgagag cagctgtcga tcccgctagt aatgtaggga
    36121 tagccggaat ttttttgatt gcccccccaa ataaaataac tagtggaatc aatgtccaag
    36181 gagagatcca aaagctcgct ttcatgtttt ggatcatttc attgatacta gctagatcgg
    36241 ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa cgaaaggacg aatgctggga
    36301 aatccgtata acgcatacca gcgatatgtt cgaatagatt aactcctcca atacccgctg
    36361 ccaagttggt cgtatctgat aaaggcgagc agttattccc taaaaaggca ccggaaacga
    36421 ctgcgcctgt agtcattgca tcattgaatc caagtaaatg accgatcccc aaaaaagcaa
    36481 ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt cactccgaca attgcacaaa
    36541 caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag tttaaatcca taaaccataa
    36601 tcgtagggat ggttccagat aaaatccatg aagcaactaa gacaccaatc aaaagaaaaa
    36661 tcaggattgg aatgatccct ggagtgattc ctttgatgat cccgtcatgg atctcatccc
    36721 atgtgaactt tttaaatctg ccataaaaca acaaacacat gaaagctgca aggatcggga
    36781 tatgtggcga gagctggaat ccgatgatca gtactcctag gagtgccaac aacacgatca
    36841 atacagcgat actttctttt aaagaaatat ttttattcat cgtcgatctc ctatttcaaa
    36901 tgaaaaggcc ctgccatgaa aaaactattc acttggcagg acctcctact aaatcagcga
    36961 caaaacgtcg gctgaaagac ttattttttt tatttttctt ctaaactttt ttctacctca
    37021 atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa catgaccact
    37081 gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt agcaaatacg
    37141 acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc gtttccgttt
    37201 ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt aagcaatgaa
    37261 acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt aagtgtcaag
    37321 aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga aataacgatt
    37381 gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg gaatccgaat
    37441 tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg tccagtaacc
    37501 caagctgata attgtaaaag cacaccaata aataccatga agctgaagat atttccgatc
    37561 acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat atttgctaat
    37621 tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac attcaataaa
    37681 actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc gtctttcaaa
    37741 cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat accagctaag
    37801 tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga aaagtcacct
    37861 agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa aacgaacatg
    37921 atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac ttttgccata
    37981 tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca acctaaaacg
    38041 aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa
    38101 gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca tgcaacgaca
    38161 aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc ttcttgagga
    38221 agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa caaaactgca
    38281 aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat actccgaacc
    38341 gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt acttacttca
    38401 gatttactca ccgttgacac tctccttgat tttttggata aaataacggt gtctttgaag
    38461 tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt tgagtctatt
    38521 gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc ttaggtctcc
    38581 ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct gttttctaac
    38641 atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag attttttcca
    38701 atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt tcttctttgt
    38761 tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca cggttccatt
    38821 cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca tagtctggga
    38881 tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct ttcacgtatg
    38941 aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca tcgttgcctt
    39001 tttctttgaa tacatagtct accatattga aatctggata agttaatgga tgaacttcga
    39061 tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac
    39121 cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt aagacatggt
    39181 gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag atataagccc
    39241 caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag taagagataa
    39301 cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga acatagccca
    39361 ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat gcactgtgta
    39421 cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa tgaacatctt
    39481 ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc
    39541 cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct aatacgcgac
    39601 gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat cctacaacac
    39661 cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt ttttcaagtt
    39721 cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga
    39781 taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga accaaccatt
    39841 ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt tcgtcgattt
    39901 tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc attaattccc
    39961 aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc ggcaatgatt
    40021 tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca gcaacgatgt
    40081 ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact tcttcttcca
    40141 tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat agcatagcaa
    40201 aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc cagtaacgtc
    40261 cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct gatagaacat
    40321 cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct ggcgtaataa
    40381 taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc accaattcat
    40441 tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat agggcattta
    40501 ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa aatatttaaa
    40561 ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt ttggcctgat
    40621 ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta
    40681 aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc
    40741 agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc
    40801 aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc
    40861 ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa
    40921 atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg
    40981 acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct
    41041 tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc
    41101 tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc
    41161 actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg
    41221 ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa
    41281 tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa
    41341 tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt
    41401 tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctactt ctggtccttc
    41461 taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat
    41521 ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcagaatttg
    41581 gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc
    41641 taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat
    41701 ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt
    41761 tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac
    41821 acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat
    41881 atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc
    41941 gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc
    42001 aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa
    42061 ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct
    42121 gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg
    42181 gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca
    42241 acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat
    42301 gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc
    42361 tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga
    42421 gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct
    42481 tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact
    42541 ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc
    42601 ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg
    42661 gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt
    42721 ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa
    42781 aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca
    42841 aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt
    42901 aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa
    42961 ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg
    43021 gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact
    43081 gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat
    43141 ttaaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta
    43201 cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg
    43261 gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc
    43321 tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc
    43381 tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca
    43441 ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa
    43501 acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg
    43561 agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta
    43621 aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt
    43681 ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa
    43741 aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca
    43801 ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc
    43861 attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc
    43921 catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca
    43981 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    44041 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    44101 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    44161 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    44221 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    44281 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    44341 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    44401 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    44461 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    44521 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    44581 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    44641 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
    44701 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
    44761 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
    44821 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
    44881 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
    44941 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
    45001 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
    45061 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
    45121 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
    45181 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
    45241 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
    45301 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
    45361 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
    45421 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
    45481 gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac tcgatgaatt gcgtcatttg
    45541 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
    45601 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
    45661 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
    45721 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
    45781 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
    45841 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
    45901 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
    45961 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
    46021 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
    46081 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
    46141 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
    46201 cttttagcaa tatcgatcag ctgtacgatg atttttttcc aagcagattc gatcactgca
    46261 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
    46321 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
    46381 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
    46441 atgtggaatc ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt
    46501 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
    46561 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
    46621 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
    46681 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
    46741 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
    46801 gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca
    46861 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
    46921 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
    46981 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
    47041 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
    47101 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatgggt
    47161 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
    47221 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
    47281 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
    47341 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
    47401 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
    47461 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
    47521 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
    47581 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
    47641 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
    47701 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
    47761 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
    47821 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
    47881 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
    47941 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
    48001 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
    48061 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
    48121 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
    48181 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
    48241 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
    48301 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
    48361 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
    48421 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
    48481 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
    48541 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
    48601 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
    48661 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
    48721 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
    48781 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
    48841 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
    48901 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
    48961 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
    49021 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
    49081 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
    49141 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
    49201 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
    49261 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
    49321 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
    49381 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
    49441 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
    49501 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
    49561 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
    49621 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
    49681 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
    49741 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
    49801 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
    49861 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
    49921 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
    49981 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
    50041 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
    50101 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
    50161 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
    50221 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
    50281 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
    50341 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
    50401 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
    50461 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
    50521 attcttaacg atcagcaaaa ctccttttcc actatccgct tccttaatag ccgtctgaat
    50581 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
    50641 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
    50701 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
    50761 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
    50821 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
    50881 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
    50941 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
    51001 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
    51061 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
    51121 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
    51181 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
    51241 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
    51301 t
//
LOCUS       DASJOX010000008        48639 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJOX010000008 DASJOX010000000
VERSION     DASJOX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.008"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..812)
                     /locus_tag="U4A12_000420"
     CDS             complement(<1..812)
                     /locus_tag="U4A12_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1772833.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFR"
     gene            complement(785..1465)
                     /locus_tag="U4A12_000421"
     CDS             complement(785..1465)
                     /locus_tag="U4A12_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1772834.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1488..2426)
                     /locus_tag="U4A12_000422"
     CDS             complement(1488..2426)
                     /locus_tag="U4A12_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN1772835.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2671..3534)
                     /locus_tag="U4A12_000423"
     CDS             complement(2671..3534)
                     /locus_tag="U4A12_000423"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN1772836.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3676..6249)
                     /gene="mprF"
                     /locus_tag="U4A12_000424"
     CDS             complement(3676..6249)
                     /gene="mprF"
                     /locus_tag="U4A12_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1772837.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6390..7196
                     /gene="proC"
                     /locus_tag="U4A12_000425"
     CDS             6390..7196
                     /gene="proC"
                     /locus_tag="U4A12_000425"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN1772838.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7275..8831)
                     /gene="rny"
                     /locus_tag="U4A12_000426"
     CDS             complement(7275..8831)
                     /gene="rny"
                     /locus_tag="U4A12_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN1772839.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9242..10291)
                     /gene="recA"
                     /locus_tag="U4A12_000427"
     CDS             complement(9242..10291)
                     /gene="recA"
                     /locus_tag="U4A12_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN1772840.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10400..11641)
                     /locus_tag="U4A12_000428"
     CDS             complement(10400..11641)
                     /locus_tag="U4A12_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN1772841.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11881..12459)
                     /gene="pgsA"
                     /locus_tag="U4A12_000429"
     CDS             complement(11881..12459)
                     /gene="pgsA"
                     /locus_tag="U4A12_000429"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN1772842.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12668..13591)
                     /locus_tag="U4A12_000430"
     CDS             complement(12668..13591)
                     /locus_tag="U4A12_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772843.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14330..15616)
                     /locus_tag="U4A12_000431"
     CDS             complement(14330..15616)
                     /locus_tag="U4A12_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1772844.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15609..16877)
                     /locus_tag="U4A12_000432"
     CDS             complement(15609..16877)
                     /locus_tag="U4A12_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1772845.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17129..17590
                     /gene="mscL"
                     /locus_tag="U4A12_000433"
     CDS             17129..17590
                     /gene="mscL"
                     /locus_tag="U4A12_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN1772846.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17650..18096)
                     /locus_tag="U4A12_000434"
     CDS             complement(17650..18096)
                     /locus_tag="U4A12_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1772847.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18189..18434)
                     /locus_tag="U4A12_000435"
     CDS             complement(18189..18434)
                     /locus_tag="U4A12_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN1772848.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18549..19127)
                     /locus_tag="U4A12_000436"
     CDS             complement(18549..19127)
                     /locus_tag="U4A12_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1772849.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19204..19734)
                     /locus_tag="U4A12_000437"
     CDS             complement(19204..19734)
                     /locus_tag="U4A12_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN1772850.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19738..20598)
                     /gene="rlmB"
                     /locus_tag="U4A12_000438"
     CDS             complement(19738..20598)
                     /gene="rlmB"
                     /locus_tag="U4A12_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN1772851.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20591..20986)
                     /locus_tag="U4A12_000439"
     CDS             complement(20591..20986)
                     /locus_tag="U4A12_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN1772852.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20983..22395)
                     /locus_tag="U4A12_000440"
     CDS             complement(20983..22395)
                     /locus_tag="U4A12_000440"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN1772853.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22392..22928)
                     /gene="cysE"
                     /locus_tag="U4A12_000441"
     CDS             complement(22392..22928)
                     /gene="cysE"
                     /locus_tag="U4A12_000441"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN1772854.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23014..23253)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23250..24710)
                     /locus_tag="U4A12_000442"
     CDS             complement(23250..24710)
                     /locus_tag="U4A12_000442"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN1772855.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24880..26037)
                     /locus_tag="U4A12_000443"
     CDS             complement(24880..26037)
                     /locus_tag="U4A12_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN1772856.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26071..27441)
                     /gene="radA"
                     /locus_tag="U4A12_000444"
     CDS             complement(26071..27441)
                     /gene="radA"
                     /locus_tag="U4A12_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN1772857.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27504..28031)
                     /locus_tag="U4A12_000445"
     CDS             complement(27504..28031)
                     /locus_tag="U4A12_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN1772858.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28261..29067
                     /gene="proB"
                     /locus_tag="U4A12_000446"
     CDS             28261..29067
                     /gene="proB"
                     /locus_tag="U4A12_000446"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN1772859.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29064..30311
                     /locus_tag="U4A12_000447"
     CDS             29064..30311
                     /locus_tag="U4A12_000447"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN1772860.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30387..30731
                     /locus_tag="U4A12_000448"
     CDS             30387..30731
                     /locus_tag="U4A12_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772861.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30848..31174)
                     /locus_tag="U4A12_000449"
     CDS             complement(30848..31174)
                     /locus_tag="U4A12_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772862.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31789..32991)
                     /locus_tag="U4A12_000450"
     CDS             complement(31789..32991)
                     /locus_tag="U4A12_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1772863.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33108..33623
                     /locus_tag="U4A12_000451"
     CDS             33108..33623
                     /locus_tag="U4A12_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1772864.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33822..34268)
                     /locus_tag="U4A12_000452"
     CDS             complement(33822..34268)
                     /locus_tag="U4A12_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1772865.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34315..35793)
                     /gene="msr(C)"
                     /locus_tag="U4A12_000453"
     CDS             complement(34315..35793)
                     /gene="msr(C)"
                     /locus_tag="U4A12_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1772866.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36147..36776)
                     /locus_tag="U4A12_000454"
     CDS             complement(36147..36776)
                     /locus_tag="U4A12_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1772867.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36760..37224)
                     /locus_tag="U4A12_000455"
     CDS             complement(36760..37224)
                     /locus_tag="U4A12_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1772868.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37711..38979)
                     /locus_tag="U4A12_000456"
     CDS             complement(37711..38979)
                     /locus_tag="U4A12_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1772869.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39210..39947)
                     /locus_tag="U4A12_000457"
     CDS             complement(39210..39947)
                     /locus_tag="U4A12_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1772870.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40014..40583)
                     /locus_tag="U4A12_000458"
     CDS             complement(40014..40583)
                     /locus_tag="U4A12_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN1772871.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40711..41583
                     /locus_tag="U4A12_000459"
     CDS             40711..41583
                     /locus_tag="U4A12_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1772872.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41925..42797
                     /locus_tag="U4A12_000460"
     CDS             41925..42797
                     /locus_tag="U4A12_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1772873.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42794..44752
                     /locus_tag="U4A12_000461"
     CDS             42794..44752
                     /locus_tag="U4A12_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1772874.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45084..45935)
                     /locus_tag="U4A12_000462"
     CDS             complement(45084..45935)
                     /locus_tag="U4A12_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1772875.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46058..46525
                     /locus_tag="U4A12_000463"
     CDS             46058..46525
                     /locus_tag="U4A12_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN1772876.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46788..47270)
                     /locus_tag="U4A12_000464"
     CDS             complement(46788..47270)
                     /locus_tag="U4A12_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1772877.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47370..48233)
                     /locus_tag="U4A12_000465"
     CDS             complement(47370..48233)
                     /locus_tag="U4A12_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1772878.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13898 a        10422 c         8687 g        15632 t
ORIGIN      
        1 cttctgaagg caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag
       61 actaatgtga ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac
      121 agcatcccta gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct
      181 aatcgcatac cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca
      241 atcgggatca tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa
      301 tgaacgggta gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc
      361 cgtttgaata aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact
      421 tgctcccatg taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa
      481 ttccctccgc tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg
      541 gcaatggaga tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct
      601 gctaatacaa cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac
      661 gctacccctg ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc
      721 actaaaaaca cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct
      781 cttctcatga attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga
      841 tctgtctgtc cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt
      901 tcccatggac atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat
      961 ccatacctgt tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg
     1021 ctaaactgat tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat
     1081 cccacatccc tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac
     1141 gcttgaacca gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag
     1201 gaaaacctgc attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc
     1261 cttcttgatt tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg
     1321 atgctttcac caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac
     1381 ccgcatctac atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca
     1441 aatcagatat ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt
     1501 tcttttaaag ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt
     1561 tgttcattag tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc
     1621 gacaatgtct cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg
     1681 tttttcttca caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt
     1741 gaaacgcccg aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca
     1801 gcatgctgag tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga
     1861 tccagtgctt caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta
     1921 tctggatatt tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc
     1981 ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct
     2041 tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc
     2101 aaggtctgtt tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa
     2161 ctattataca cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg
     2221 aaatcatgcg gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg
     2281 atctcttttg taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt
     2341 ttgtctgctt ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc
     2401 gtagctgtca aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt
     2461 tacgatttat taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca
     2521 tcaatttctg atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc
     2581 tcatatctag ccatttcact acaataaaaa actgaagaat accattttag gattcctcag
     2641 tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc
     2701 ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa
     2761 ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat
     2821 acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt
     2881 tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg
     2941 gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac
     3001 actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat
     3061 ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt
     3121 accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg
     3181 gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac
     3241 tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag
     3301 caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt
     3361 ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac
     3421 gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata atacatccag
     3481 tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa
     3541 agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta
     3601 gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac
     3661 aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc
     3721 tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt
     3781 cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc
     3841 agactggaag gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg
     3901 aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc
     3961 cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt cagctaattc
     4021 ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg
     4081 ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga
     4141 taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag
     4201 ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca
     4261 agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga
     4321 caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc
     4381 caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc
     4441 taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc
     4501 aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg
     4561 atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct
     4621 tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact
     4681 gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga
     4741 gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg
     4801 atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac
     4861 gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa
     4921 ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca agacatatac
     4981 caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa
     5041 agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag
     5101 taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa
     5161 taatacttga aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat
     5221 catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa
     5281 cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa
     5341 tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac
     5401 tactgctaca tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa
     5461 agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat
     5521 aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac
     5581 cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg
     5641 ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc
     5701 taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat
     5761 aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag
     5821 tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact
     5881 agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt
     5941 tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa
     6001 gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag
     6061 aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca
     6121 actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa
     6181 aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt
     6241 atttttcaca ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct
     6301 tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa
     6361 gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata
     6421 tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt
     6481 tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg
     6541 catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac
     6601 caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca
     6661 tttctattgc ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg
     6721 cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga
     6781 tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag
     6841 caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg
     6901 gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga
     6961 atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc
     7021 aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg
     7081 gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca
     7141 tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat
     7201 agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc
     7261 ttttgtattt tttattattt agcataatca gttgcacgag tttcccgaat aacgattact
     7321 ttgatgtgtc caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa
     7381 cgaacagcgt ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca
     7441 gcttgaactg cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt
     7501 cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag
     7561 agcgcatctg ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga
     7621 tgagaagcga tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca
     7681 ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc
     7741 aacccggcac gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct
     7801 aattttgcaa cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc
     7861 agacgtccga tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct
     7921 gcctcttccc cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt
     7981 tcttcgatac gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt
     8041 gcgatttctc ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg
     8101 atgattaaat cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca
     8161 atgatacgac ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa
     8221 acttgatctg ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct
     8281 gcttcttctt tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc
     8341 tcttcttcag tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt
     8401 ttttctagtt ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca
     8461 attaattgct gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt
     8521 tctaacgagt catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga
     8581 gattttaatt ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga
     8641 ttttcttcct tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca
     8701 ctatcgatga ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac
     8761 tttgcgacca taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg
     8821 ttgaatacca taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt
     8881 ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac
     8941 atgcacaact ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa
     9001 cggctttcta cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata
     9061 ggtaaacctt taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat
     9121 ttttatttta tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa
     9181 aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt
     9241 tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt
     9301 cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat
     9361 gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag
     9421 aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc
     9481 cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg
     9541 ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt
     9601 tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc
     9661 ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga
     9721 tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag
     9781 acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc
     9841 gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta
     9901 aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat
     9961 cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga
    10021 aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt
    10081 tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac
    10141 ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt
    10201 tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag
    10261 catctaaggc tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc
    10321 agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc
    10381 gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt
    10441 gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa
    10501 ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg
    10561 tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg
    10621 cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct gctaatagct
    10681 ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg
    10741 gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca
    10801 aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg
    10861 ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt
    10921 cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc
    10981 gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa
    11041 tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt
    11101 tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca
    11161 caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg
    11221 tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga
    11281 taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg
    11341 ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag
    11401 ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt
    11461 cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa
    11521 ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat
    11581 tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca
    11641 ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga
    11701 gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct
    11761 cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga
    11821 aattatccct tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac
    11881 ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat
    11941 agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa
    12001 tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt
    12061 tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt
    12121 gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc
    12181 aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt
    12241 gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat
    12301 actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc
    12361 tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat
    12421 acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc
    12481 tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt
    12541 attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac
    12601 acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt
    12661 tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt
    12721 cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac
    12781 caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg
    12841 ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac catacacgat
    12901 tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg
    12961 cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg
    13021 ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct
    13081 cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac
    13141 gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata
    13201 aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt
    13261 ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc
    13321 ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt
    13381 gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga
    13441 ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt
    13501 ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat
    13561 cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt
    13621 gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg
    13681 ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt
    13741 gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa
    13801 tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta
    13861 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc
    13921 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt
    13981 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt
    14041 gttccagaag tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt
    14101 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac
    14161 ttcgtttgat tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc
    14221 tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct
    14281 actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc
    14341 tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt
    14401 ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata
    14461 aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc
    14521 caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga
    14581 atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc
    14641 tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga
    14701 taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga
    14761 acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt
    14821 ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt
    14881 gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga
    14941 aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac
    15001 caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt
    15061 cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt
    15121 gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat
    15181 acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt
    15241 aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag
    15301 aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc
    15361 caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg
    15421 caattccatc tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga
    15481 tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca
    15541 aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat
    15601 attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt
    15661 tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct
    15721 tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct
    15781 tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt
    15841 tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt
    15901 acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta
    15961 tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt
    16021 ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt
    16081 tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca
    16141 cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag atctggataa
    16201 actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca
    16261 acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac
    16321 gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt
    16381 tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta
    16441 tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac
    16501 tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc
    16561 gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg
    16621 tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc
    16681 tctgcaagtt gcgcacttaa ctttgtctga tccgaataat tcaaactatt tgtttccaac
    16741 atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg
    16801 ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct
    16861 gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt
    16921 taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa
    16981 ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa
    17041 aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa
    17101 agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg
    17161 tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt
    17221 aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac
    17281 aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt
    17341 cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt
    17401 cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca
    17461 agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct
    17521 tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac
    17581 ttctaaataa taaagaatct aaaaaaaaag aaagccgatt ttcagtgtcg gctttctttt
    17641 tttagattct taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc
    17701 caaatacaca tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc
    17761 ttgtgcgccg atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc
    17821 ttgaacaatc tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc
    17881 aaaatggata ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt
    17941 atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca tcaacttctt gataaggaca
    18001 ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt
    18061 tgacaattct tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt
    18121 attcttagca taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg
    18181 tttgcttttt aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct
    18241 gaatagctgt aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat
    18301 actgaaggat aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc
    18361 tgagcaacca acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct
    18421 aaagttgttg gcatacagtt cacctctact atcaactata ccacatattt ttaaaatttt
    18481 caagtttacc ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt
    18541 tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc
    18601 ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa
    18661 tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg
    18721 caattttcga ttgcttcttt taataaaaga tgctgaattg ctaaaggttc ttgcaatgtg
    18781 atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct
    18841 ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt
    18901 ataacgcttt tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat
    18961 gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa
    19021 taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat
    19081 gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc
    19141 cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat
    19201 atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc
    19261 ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc
    19321 tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc
    19381 tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt
    19441 caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc
    19501 tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc ccggaactag
    19561 ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg
    19621 atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc
    19681 cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta
    19741 ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt
    19801 taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa
    19861 tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt
    19921 ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt
    19981 gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc
    20041 cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat
    20101 cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc
    20161 ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat
    20221 cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac
    20281 gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg
    20341 ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa
    20401 gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa
    20461 attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt
    20521 tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt
    20581 tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt
    20641 ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg
    20701 cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc
    20761 ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa
    20821 tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg
    20881 gttttgtttc tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc
    20941 caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca
    21001 tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat
    21061 ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg
    21121 ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca
    21181 ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag
    21241 ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa
    21301 atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc
    21361 cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt
    21421 attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat
    21481 cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt
    21541 gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc
    21601 gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc
    21661 ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg
    21721 gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc
    21781 gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca
    21841 taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc
    21901 tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg
    21961 ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg
    22021 aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggttc
    22081 gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg
    22141 tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt
    22201 cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc
    22261 aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata
    22321 cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt
    22381 gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct
    22441 ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg
    22501 attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg
    22561 gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt
    22621 acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc
    22681 attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca
    22741 gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat
    22801 aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc
    22861 tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac
    22921 caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa
    22981 agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca
    23041 cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta
    23101 ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga
    23161 ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc
    23221 agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat
    23281 gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct
    23341 gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga
    23401 acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt
    23461 gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct
    23521 tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca
    23581 tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca
    23641 tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat
    23701 gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg
    23761 aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt
    23821 tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt
    23881 gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca
    23941 cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt
    24001 ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg
    24061 tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa
    24121 ttataagttg gcataccatc gcgtttttgg atgacaaagt cgccaccgat attatcggat
    24181 tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca
    24241 cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc
    24301 aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt
    24361 tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc
    24421 tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct
    24481 ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg
    24541 cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag
    24601 tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata
    24661 tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc
    24721 ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata
    24781 tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca
    24841 actggttgaa tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt
    24901 tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat
    24961 catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat
    25021 gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg
    25081 ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac
    25141 tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga
    25201 gactttattc aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt
    25261 gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga
    25321 tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg
    25381 tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat
    25441 catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat
    25501 cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag
    25561 ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac
    25621 tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat
    25681 catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt
    25741 cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata
    25801 agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt
    25861 ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt
    25921 attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga
    25981 aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt
    26041 ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc
    26101 caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt
    26161 tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat
    26221 atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg
    26281 ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc
    26341 tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag
    26401 caacccagct cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc
    26461 tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac
    26521 caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag
    26581 gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat
    26641 ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg
    26701 aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat
    26761 tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc
    26821 atttgtttta gcggttttga gtagttctgc agtggtttct cttacttggc tgacacttcc
    26881 tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc
    26941 aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata
    27001 aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc
    27061 ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa
    27121 cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac
    27181 cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt
    27241 tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg
    27301 agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca
    27361 atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg
    27421 tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat
    27481 caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga
    27541 gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc
    27601 tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg
    27661 ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac
    27721 actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct
    27781 cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct
    27841 gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc
    27901 cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt
    27961 gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct
    28021 ctttttctca ccggttttcc tccctaaata aactactcct attctaacat atccgaggct
    28081 gtaaaagact ttctttacaa attcttcaag aaattctgct atcttaaagt aaaccattaa
    28141 ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta catgacttta
    28201 ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg atatttgatt gaggtgtcac
    28261 atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac aagtacattg
    28321 atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt tgtcctttct
    28381 gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt aggcgttggt
    28441 atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca agctgcggcg
    28501 gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt atataatcaa
    28561 cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag tcgtaaaaat
    28621 gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt gaacgaaaac
    28681 gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa tgatcagctg
    28741 tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc tgatatcgac
    28801 gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg ctatatcgat
    28861 aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt tggaacaggc
    28921 ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag cgcaatgatt
    28981 ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa agaagtaggt
    29041 actttattta aggaggactt gaaatgactg acttgatgca attaggacaa caggcaaaag
    29101 atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta cttgttcaaa
    29161 tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat aaagacttag
    29221 aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg
    29281 agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac gatcctattg
    29341 gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa aagcgcgtgc
    29401 cactaggcgt gatcggcatc atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta
    29461 gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa gcattcttct
    29521 cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc gaatctcctt
    29581 atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg atgcgattga
    29641 cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa cgagtcaaag
    29701 aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta tatatcgata
    29761 aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat
    29821 ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct tttttcttgc
    29881 cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt
    29941 tagaatatct agagacggct gctttagcag aagacacaga ttgggatact gaatatctag
    30001 attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca catatcaatc
    30061 ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct agtcaacgtt
    30121 tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt tttacagacg
    30181 gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg catgcccgtg
    30241 gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc gatggacaaa
    30301 tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg
    30361 ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt tttatttacc
    30421 ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt ttctttcaat
    30481 tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg
    30541 ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta tgcttattag
    30601 cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt acgctactga
    30661 ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac ttgaatcgga
    30721 ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg caaaaaactg
    30781 ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc atataaacgg
    30841 cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt
    30901 caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc atcgtttttt
    30961 ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga tacataccag
    31021 ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt ggcaattctt
    31081 tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg
    31141 tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt ctttttctta
    31201 aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag aaaaaaaggg
    31261 aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc ttctgccaac
    31321 tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg aaattgttcc
    31381 ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt tcaatcagct
    31441 gaccctcggt attagctcaa aaaacggtaa aatatgagaa gctatttttc cgttttgttt
    31501 ctctaatact taggataaaa cgctgatttt acaacctctg ttaaacggtg ttcaatcagc
    31561 tgtccttcgg tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt
    31621 ctctaatact caggacaaaa cgctgatttt acaacctcct gataaacggt gttcaaaagc
    31681 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    31741 tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc
    31801 atttgtagca aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc
    31861 acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa
    31921 tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg
    31981 ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa
    32041 aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa
    32101 taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa
    32161 cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc
    32221 aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc
    32281 aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt
    32341 atgattgctt aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt
    32401 ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa
    32461 aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat
    32521 tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat
    32581 actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc
    32641 acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac
    32701 gatgataaac aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc
    32761 tgtaaatagc atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc
    32821 agctggcgta gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac
    32881 tcccataaaa gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt
    32941 ttcttcattt gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc
    33001 tctattagca tcatacatga tttattttac atgtcaatat gcacatgtaa aacgtggaaa
    33061 ctttactgtg atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag
    33121 cctatatatt attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc
    33181 tgatcacaat tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag
    33241 accagcaggg ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc
    33301 acttgactga tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc
    33361 ctacgcaaaa agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa
    33421 cagctactgg actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc
    33481 aaaaaaaatt gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga
    33541 tccagcgcag aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac
    33601 aaatgcagca gttttttgag taagggataa acagaacctt tctagaaata tctattgaag
    33661 agcaaattat cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac
    33721 aggccaaaac ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac
    33781 aaaaagcatg ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt
    33841 gcttttagca cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca
    33901 atcacattca ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt
    33961 tccatgatca caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa
    34021 atagttaaga tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt
    34081 ttcgccagca caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact
    34141 agaccaatca acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc
    34201 atccccaaga ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt
    34261 gtaggcaatc tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa
    34321 atttctcgta agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt
    34381 acgctcgaca aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat
    34441 tagcttctct aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag
    34501 caacacatta cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc
    34561 acttaatgta cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa
    34621 actacggaca agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc
    34681 ttcagatgtc atttgataac ccttttgctg atatacttga aagcttactt tcggagagag
    34741 aaggattcct tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt
    34801 ttcaccgaca atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa
    34861 tgttctccca cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag
    34921 ttcaagagat tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc
    34981 tcctattttt tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac
    35041 actgtctttt tgtttagagg aagacaaccg atccagtctg atttgttgct ttttcttttt
    35101 actggacact ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa
    35161 tcgttttttc tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc
    35221 cttttgagtg agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac
    35281 ttcccagatt ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag
    35341 cgtaccataa taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg
    35401 ggttgttggc tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc
    35461 gatctttcgt tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg
    35521 gaaatgactg gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg
    35581 caggtagttg aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc
    35641 tattagtttg agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc
    35701 ttgctgatag acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg
    35761 tagatttgtt atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa
    35821 acaacaatta tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac
    35881 atcttttcct actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc
    35941 acctattcta ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata
    36001 catgtctttt gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc
    36061 agtcattttt ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata
    36121 gctactgaat agtaactgaa aaccttttat tcgaacatag tgatcttttc tggtgctaaa
    36181 cgaacagact cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca
    36241 taacggtctt tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt
    36301 ggatttgttt catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta
    36361 gaatcgactt tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg
    36421 atcgcagcta attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg
    36481 ttgtagattt cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca
    36541 gaagaagtat cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct
    36601 tcctcgcttt cagcgaccac aaagcgccat ggtttcatat tgacagaaga aggtgcagtc
    36661 gtcgcttctt tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat
    36721 acgcgaacgg atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt
    36781 attaacctct ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag
    36841 tgttagaaat tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc
    36901 tttatggtcc atttcacaat gcttgatttt cttctgggct ttttctgtta gcgctacata
    36961 cacttcccgg ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac
    37021 cttcagatga cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg
    37081 tagcaatgga ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc
    37141 gatattttct tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag
    37201 gtgactaatc tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat
    37261 tgacttgtca ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa
    37321 aaaacgctct caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa
    37381 atgataaaga aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga
    37441 tagctcttct gcagtagata attttacacc gccaaaaatt tccgaaatcg ccttttgcaa
    37501 gaatcccgtc gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg
    37561 acaagatgtt gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta
    37621 ataagttcaa ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt
    37681 gatgcagggc agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata
    37741 tctccagccc atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt
    37801 accagttttt tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa
    37861 acttagacct tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat
    37921 tgtgtaatga tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt
    37981 tggtgcttgc caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc
    38041 tccggcattc aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg
    38101 agaagttgaa gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt
    38161 caatgtagaa ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc
    38221 tttgtaagag ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa
    38281 agaagtcgtc ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt
    38341 tctaaaagcg gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt
    38401 aacagattgg ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc
    38461 caaagtactg ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata
    38521 taagtcattg gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg
    38581 ttgagaatta gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga
    38641 caaatctgct tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc
    38701 ttctgcattc tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg
    38761 ttctgttgtt tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga
    38821 ctgcaaagta ctttctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt
    38881 tgcgacagtt tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc
    38941 taaaagaata acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat
    39001 gaaacttttc ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt
    39061 aaataacatt tgtttctttt taataaattt gctttttctg cataaaaaga cgtaaatgaa
    39121 gaatattctt ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt
    39181 aattctctct ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc
    39241 atgttgctga cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata
    39301 tcttcagatg ttaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg
    39361 tcgctgatgg tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg
    39421 atgttatttt gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt
    39481 gttccgcaat aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa
    39541 atgatatgcc cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac
    39601 acacccatga tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat
    39661 ggagcggttg gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc
    39721 atcgctaaat cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata
    39781 tatagctcag cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca
    39841 gctataacta gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg
    39901 cttgaagcgc ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc
    39961 tgttttgaaa aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata
    40021 atccgttcga caggtttgac gattttggca gggattcctg caacgatggt attgtctggg
    40081 acatctttcg taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct
    40141 ggcagaatag ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact
    40201 gacaagcctc ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga
    40261 ggaccaatca atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga
    40321 cgattgataa agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc
    40381 agcagaacag tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg
    40441 atatctgctg gttcatggta cccagtattc agcgctgcta aaggctcttc gttttccgct
    40501 tggatctgat ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt
    40561 tgtttttcga tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg
    40621 gcttatgatg tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg
    40681 gtatgctttt ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta
    40741 gcagtagcta gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct
    40801 actctctcaa aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcga
    40861 ggctcccgag agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa
    40921 atcttgtcgt tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga
    40981 gaaatctaca ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat
    41041 tcgttgatca aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat
    41101 atcatagaca aactggatag cggattactt gattttggac tcgtaatcga accgacaaac
    41161 aaacaaaaat accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat
    41221 cggaatcatc ctttagcaaa gcaaacagtc atccatccgc aggaactgaa aactgttcct
    41281 ttgatcattt cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc
    41341 atagatcagt tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc
    41401 gataacggga cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa
    41461 aaagtattca tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa
    41521 gaccagcctc tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt
    41581 taagtagagt ttttttcctt cgctttcagc tttttaacaa cgaataagtt aagcaaaaga
    41641 tccaatgatt aaataacata agtttcttaa tttgttcctt ccttttattt ctgataattc
    41701 cagtaaatta ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt
    41761 gattacgtta acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct
    41821 gaggaacatg gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact
    41881 ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga
    41941 tctttaatca aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa
    42001 aagggattgg atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga
    42061 tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg
    42121 aacgcttttt atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa
    42181 agttgaatga acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca
    42241 ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg
    42301 atgaattcag tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat
    42361 atacttatga cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga
    42421 acaataacca tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg
    42481 aatcagaatt ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg
    42541 tgaatcattt acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca
    42601 cggaaatcgt agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa
    42661 tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact
    42721 tggccatcca tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg
    42781 aggaggaaaa aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt
    42841 tgcggtctta cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag
    42901 taacgacatt gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc
    42961 tttgctgttt gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc
    43021 cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc
    43081 tactttattg aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt
    43141 atttattggt attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc
    43201 aaaattaccg atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc
    43261 ctttgtcatg ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc
    43321 tttagtttct tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg
    43381 cttcttcaac cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg
    43441 gtttgacgta gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg
    43501 cgtaacagga atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg
    43561 agcatatgct atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat
    43621 gttagcagca gcttttgctt cattctttac tggtgtaaca gaaccacttg aattctcatt
    43681 tatgttcgtt gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc
    43741 agtgagtgca ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta
    43801 cttcctatct ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt
    43861 agtaacagca gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat
    43921 gactcctggc cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga
    43981 caataaatat gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt
    44041 aacttcgatc gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat
    44101 cgatcaaaat aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa
    44161 tgcgcaagtc atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca
    44221 tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc
    44281 cggtggaacg atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac
    44341 tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc
    44401 agaaaatgga gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa
    44461 acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa
    44521 cactgttgac ttaaaaggcg aaccattcac tttatatgta aaagaaggtc aaaaagtcgc
    44581 aagaggtcaa ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac
    44641 ggatatggtc gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc
    44701 acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt
    44761 cttccccaga cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc
    44821 tcctttttcc gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagccaa
    44881 acgtaacata tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc
    44941 ttttttacaa cctctgataa acggtgttca aaaaccagtt tatcggtatg aagtcaaatt
    45001 tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tcatactcaa aactaatcgg
    45061 ctttttcaca acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca
    45121 agagaagtta atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga
    45181 tcctttttaa tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg
    45241 gcttctttta gattttcgac aaacgctgca tcacttgctt caataatttc taacttttta
    45301 cctgtcgcac gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat
    45361 tctaagattt ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca
    45421 cgtttcaatg cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt
    45481 aaagcctgcc caatgatagg taattcattc tctacatacc aattattccg taaaaatgta
    45541 tgggaaatac ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaataca
    45601 ctgatcgagt gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct
    45661 tgtgctgcct tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca
    45721 aaaagcaatc gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca
    45781 ccaatacgta tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact
    45841 agtacataga tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca
    45901 aaacctccag ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat
    45961 ttgttaagat ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa
    46021 gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag
    46081 tgatgcaatc catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag
    46141 tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca
    46201 gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa
    46261 aagtccagat cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct
    46321 tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc
    46381 tttagaagaa agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac
    46441 acttgatagt ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac
    46501 aggcctcgta gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa
    46561 tcgtctacat aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc
    46621 tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta
    46681 gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc
    46741 ctctctaatg ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg
    46801 actaccggta tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca
    46861 gcatttggac ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc
    46921 gtattcgtca aatcatttct taattcttca ggcgattctg ctaaagcgat tacaacaaga
    46981 tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg gaacatcctg actcgtttga
    47041 acaccgacat agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga
    47101 ttttcagcag cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat
    47161 gcttgtttct ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca
    47221 atacccgtca ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc
    47281 ctttctatta tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc
    47341 ttttttctag ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc
    47401 tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg
    47461 actaactcat aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt
    47521 aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca
    47581 agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc
    47641 aaactgccct tttcttccaa gcgggctcca tcaaatccat aagaaacgtt gattacttga
    47701 tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc
    47761 gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca
    47821 cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg
    47881 acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct gctgatttct
    47941 gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat
    48001 ccatatctaa acgccgtttc tgtcactctt gatccatcta ggagatcgcg attagccaaa
    48061 gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg
    48121 cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct
    48181 tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc
    48241 ttcctccttg cttgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag
    48301 gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc
    48361 gtttcgtaac ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa
    48421 ttttggggaa gacacattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt
    48481 taatccgctt actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc
    48541 tgcttggatt tttctttgcg atacatcata cctcttttat caattggaac aattatttat
    48601 ttctaacaaa atcacttcat ttttgtcaga taaataaaa
//
LOCUS       DASJOX010000009        47219 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJOX010000009 DASJOX010000000
VERSION     DASJOX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.009"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(194..415)
                     /locus_tag="U4A12_000466"
     CDS             complement(194..415)
                     /locus_tag="U4A12_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772879.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(577..649)
                     /locus_tag="U4A12_000467"
     tRNA            complement(577..649)
                     /locus_tag="U4A12_000467"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(614..616),aa:Lys,seq:ctt)
     gene            725..955
                     /locus_tag="U4A12_000468"
     CDS             725..955
                     /locus_tag="U4A12_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772880.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            999..2168
                     /locus_tag="U4A12_000469"
     CDS             999..2168
                     /locus_tag="U4A12_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1772881.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            2742..3311
                     /locus_tag="U4A12_000470"
     CDS             2742..3311
                     /locus_tag="U4A12_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN1772882.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3298..4158
                     /locus_tag="U4A12_000471"
     CDS             3298..4158
                     /locus_tag="U4A12_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1772883.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4348..5052
                     /locus_tag="U4A12_000472"
     CDS             4348..5052
                     /locus_tag="U4A12_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1772884.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5057..6892
                     /gene="walK"
                     /locus_tag="U4A12_000473"
     CDS             5057..6892
                     /gene="walK"
                     /locus_tag="U4A12_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN1772885.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6889..8205
                     /locus_tag="U4A12_000474"
     CDS             6889..8205
                     /locus_tag="U4A12_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772886.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8206..9075
                     /locus_tag="U4A12_000475"
     CDS             8206..9075
                     /locus_tag="U4A12_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN1772887.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9130..9939
                     /locus_tag="U4A12_000476"
     CDS             9130..9939
                     /locus_tag="U4A12_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1772888.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10042..10671)
                     /locus_tag="U4A12_000477"
     CDS             complement(10042..10671)
                     /locus_tag="U4A12_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN1772889.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10841..12133
                     /locus_tag="U4A12_000478"
     CDS             10841..12133
                     /locus_tag="U4A12_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1772890.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12209..12871)
                     /locus_tag="U4A12_000479"
     CDS             complement(12209..12871)
                     /locus_tag="U4A12_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1772891.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13096..13380
                     /gene="groES"
                     /locus_tag="U4A12_000480"
     CDS             13096..13380
                     /gene="groES"
                     /locus_tag="U4A12_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN1772892.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13431..15056
                     /gene="groL"
                     /locus_tag="U4A12_000481"
     CDS             13431..15056
                     /gene="groL"
                     /locus_tag="U4A12_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN1772893.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(15145..16293)
                     /locus_tag="U4A12_000482"
     CDS             complement(15145..16293)
                     /locus_tag="U4A12_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1772894.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIES
                     VIADNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(16395..16979)
                     /locus_tag="U4A12_000483"
     CDS             complement(16395..16979)
                     /locus_tag="U4A12_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN1772895.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(17106..17537)
                     /locus_tag="U4A12_000484"
     CDS             complement(17106..17537)
                     /locus_tag="U4A12_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1772896.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(17555..17875)
                     /locus_tag="U4A12_000485"
     CDS             complement(17555..17875)
                     /locus_tag="U4A12_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1772897.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            18169..18309
                     /locus_tag="U4A12_000486"
     CDS             18169..18309
                     /locus_tag="U4A12_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772898.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            18607..18864
                     /locus_tag="U4A12_000487"
     CDS             18607..18864
                     /locus_tag="U4A12_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEN1772899.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(18835..19143)
                     /locus_tag="U4A12_000488"
     CDS             complement(18835..19143)
                     /locus_tag="U4A12_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772900.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            19221..19967
                     /locus_tag="U4A12_000489"
     CDS             19221..19967
                     /locus_tag="U4A12_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1772901.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            19982..20185
                     /locus_tag="U4A12_000490"
     CDS             19982..20185
                     /locus_tag="U4A12_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1772902.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            20290..20589
                     /locus_tag="U4A12_000491"
     CDS             20290..20589
                     /locus_tag="U4A12_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772903.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            20589..20759
                     /locus_tag="U4A12_000492"
     CDS             20589..20759
                     /locus_tag="U4A12_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772904.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(20818..21297)
                     /locus_tag="U4A12_000493"
     CDS             complement(20818..21297)
                     /locus_tag="U4A12_000493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772905.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            21342..22049
                     /locus_tag="U4A12_000494"
     CDS             21342..22049
                     /locus_tag="U4A12_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEN1772906.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            22228..22569
                     /locus_tag="U4A12_000495"
     CDS             22228..22569
                     /locus_tag="U4A12_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772907.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            22562..23308
                     /locus_tag="U4A12_000496"
     CDS             22562..23308
                     /locus_tag="U4A12_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1772908.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            23314..24000
                     /locus_tag="U4A12_000497"
     CDS             23314..24000
                     /locus_tag="U4A12_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772909.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            24007..24840
                     /locus_tag="U4A12_000498"
     CDS             24007..24840
                     /locus_tag="U4A12_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN1772910.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            24857..25708
                     /locus_tag="U4A12_000499"
     CDS             24857..25708
                     /locus_tag="U4A12_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1772911.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            25705..26064
                     /locus_tag="U4A12_000500"
     CDS             25705..26064
                     /locus_tag="U4A12_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN1772912.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            26079..26240
                     /locus_tag="U4A12_000501"
     CDS             26079..26240
                     /locus_tag="U4A12_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1772913.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            26237..26542
                     /locus_tag="U4A12_000502"
     CDS             26237..26542
                     /locus_tag="U4A12_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772914.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            26542..26859
                     /locus_tag="U4A12_000503"
     CDS             26542..26859
                     /locus_tag="U4A12_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN1772915.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            26856..27242
                     /locus_tag="U4A12_000504"
     CDS             26856..27242
                     /locus_tag="U4A12_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772916.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            27239..27574
                     /locus_tag="U4A12_000505"
     CDS             27239..27574
                     /locus_tag="U4A12_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772917.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            27720..28289
                     /locus_tag="U4A12_000506"
     CDS             27720..28289
                     /locus_tag="U4A12_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1772918.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            28286..28459
                     /locus_tag="U4A12_000507"
     CDS             28286..28459
                     /locus_tag="U4A12_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772919.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            28456..28761
                     /locus_tag="U4A12_000508"
     CDS             28456..28761
                     /locus_tag="U4A12_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772920.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            28785..28982
                     /locus_tag="U4A12_000509"
     CDS             28785..28982
                     /locus_tag="U4A12_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN1772921.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            28979..29185
                     /locus_tag="U4A12_000510"
     CDS             28979..29185
                     /locus_tag="U4A12_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772922.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            29185..29481
                     /locus_tag="U4A12_000511"
     CDS             29185..29481
                     /locus_tag="U4A12_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772923.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            29557..29970
                     /locus_tag="U4A12_000512"
     CDS             29557..29970
                     /locus_tag="U4A12_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1772924.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            30654..30821
                     /locus_tag="U4A12_000513"
     CDS             30654..30821
                     /locus_tag="U4A12_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772925.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            30847..31191
                     /locus_tag="U4A12_000514"
     CDS             30847..31191
                     /locus_tag="U4A12_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1772926.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            31196..31477
                     /locus_tag="U4A12_000515"
     CDS             31196..31477
                     /locus_tag="U4A12_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772927.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            31580..31894
                     /locus_tag="U4A12_000516"
     CDS             31580..31894
                     /locus_tag="U4A12_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN1772928.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            31872..33566
                     /locus_tag="U4A12_000517"
     CDS             31872..33566
                     /locus_tag="U4A12_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1772929.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            33586..34764
                     /locus_tag="U4A12_000518"
     CDS             33586..34764
                     /locus_tag="U4A12_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1772930.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            34727..35413
                     /locus_tag="U4A12_000519"
     CDS             34727..35413
                     /locus_tag="U4A12_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1772931.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            35413..36573
                     /locus_tag="U4A12_000520"
     CDS             35413..36573
                     /locus_tag="U4A12_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1772932.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            36583..37458
                     /locus_tag="U4A12_000521"
     CDS             36583..37458
                     /locus_tag="U4A12_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772933.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            37455..37766
                     /locus_tag="U4A12_000522"
     CDS             37455..37766
                     /locus_tag="U4A12_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772934.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            37756..38109
                     /locus_tag="U4A12_000523"
     CDS             37756..38109
                     /locus_tag="U4A12_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1772935.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            38099..38500
                     /locus_tag="U4A12_000524"
     CDS             38099..38500
                     /locus_tag="U4A12_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772936.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            38493..38897
                     /locus_tag="U4A12_000525"
     CDS             38493..38897
                     /locus_tag="U4A12_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772937.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            38909..39517
                     /locus_tag="U4A12_000526"
     CDS             38909..39517
                     /locus_tag="U4A12_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1772938.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            39537..39899
                     /locus_tag="U4A12_000527"
     CDS             39537..39899
                     /locus_tag="U4A12_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772939.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            39902..40084
                     /locus_tag="U4A12_000528"
     CDS             39902..40084
                     /locus_tag="U4A12_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772940.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            40101..43520
                     /locus_tag="U4A12_000529"
     CDS             40101..43520
                     /locus_tag="U4A12_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1772941.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            43571..44308
                     /locus_tag="U4A12_000530"
     CDS             43571..44308
                     /locus_tag="U4A12_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1772942.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            44318..46609
                     /locus_tag="U4A12_000531"
     CDS             44318..46609
                     /locus_tag="U4A12_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1772943.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            46633..>47219
                     /locus_tag="U4A12_000532"
     CDS             46633..>47219
                     /locus_tag="U4A12_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772944.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT        16530 a         7370 c         9790 g        13529 t
ORIGIN      
        1 tcaaggagca tattttagtt gcgccatgat ttcttactgt aaagaaggag cgataaacat
       61 tctaaaataa tttttatagt caataaaaag cgatgatttg aagttgggat aatgatactt
      121 ccgtaaggct cgccgtaaac ttggagagag gtgaaattcc tatgtgcttt tgacaaaagc
      181 agctgattaa gaattatttc catatgttga tttcaacatt ttttctatta tttttaagac
      241 caaattcagt gaggcaaata gcatcatgta aatttagttt ttctattgaa cgttctccac
      301 tttttagttt ttttatagta ctcaaattta atcctgtatg tttagagatc tcatttgcag
      361 tatggttttg taaagtttct cggattattg ttatattagc aatttcatta cgcataatta
      421 tgttcctttc ttatcaaaaa tatcaatgac tacagtgggc ttttagtgaa aaaaaggggt
      481 actacagtct cttttgaaaa caaaaaaata cagtatatac gttttctaaa tgctaagcac
      541 caacgtttac actgtattta agggtttggc acccaatggg ctgtgagggg ctcgaacccg
      601 cgacccgatg attaagagtc atctgctcta ccaactgagc taacagccca aattaatact
      661 ttgttattct aacacctaaa ataaaaaagt gcaatactta ttatcagaat tgagagtgaa
      721 ttttatggat tttcaagatt atgaaaaaat atttgaaagt tatccatcga tattgaaaat
      781 tactgattta actaaaatgt taaatattgg aaaaaataaa gcttataatt tgctgaaaga
      841 tagatcaata gaaaatataa aaattggagc aaggtacaaa atacctaaaa aagctgtcat
      901 tgagtatata ttgaaaatac aagatgaagg taaaaatcat gctataataa aatagtcaaa
      961 atttttgccg atatttcagt ttaagaggag agattgaatt gaaattaaga gcaaacataa
     1021 ttgaaaaaag aggaacatta caagtagttg tatcttataa agactctaac ggaaaaatta
     1081 gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa taaaaaacga gcagaaaaaa
     1141 ttagagatga atttttacta gaattggaaa gtcaattagc tatggaatgt gaatgtgcta
     1201 atccaaattt attgttttat gactatttgc ttaattattt ggaagtatca aaaaaacaag
     1261 tagcatttaa cacatatgtt gggtataagc attacgtcca taatcgaatt tacaagtttt
     1321 tttatcctaa aaaaattaaa ctaaataggt taaagcctta tcatttacaa gagttttatc
     1381 aatatatgtt aaatgataac tgtacagcaa atacggttat tcattatcac gcatttatac
     1441 gcaaagctct tcaagaggct gttattacag aattgataag tacaaatgta gcagataaag
     1501 taagaaagcc aaaaaagaaa caatttgttt ctcaagttta taatcaacaa gagatcttga
     1561 aattacttga cattttgtca gaagaaaaac tctatttggt agttctttta acagcatttt
     1621 atggattgcg aagaagtgaa gttttaggat taaaatggtc tgctattgat tttttaaaca
     1681 aaaaaatcat gataaaccat gtaatcatag aaaatccaga gaatatgtca cagttaataa
     1741 agaaagatca aacaaagaac tcttccagct atcgtacatt acctttggtt gaaacgattg
     1801 aacgagcatt agtaaatcaa gctaaatggc aacaagataa tcgtattctt ttaggagatg
     1861 attatatttt agaagataat gagtacgtat ttactatgga agatggacgg ttgatgaagc
     1921 ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa aaatggattg aaaaaaattc
     1981 gctttcatga cttgcgtcat tctaatgctt caataatgtt agatagtggc cagaatatga
     2041 aatccattca agaatggctg ggacatgcat cgtattcgac aactgctaat ttatacacgc
     2101 atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca tttgggttta
     2161 aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt tagaattttt
     2221 ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat
     2281 ttataaagct ttataaaact tttttcacaa atagagcatt tggttaagag tcagctgctc
     2341 taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac taagaatttt
     2401 aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta taggaaatca
     2461 ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac aaagttcttg
     2521 caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg tctccccggc
     2581 gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt ttttgttttc
     2641 atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt ttatcataag
     2701 tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc
     2761 aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg gaagaactcc
     2821 ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat ctttcacctg
     2881 tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat tacgttaaac
     2941 taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta gcatttttac
     3001 ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa gggatattgt
     3061 tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat gaacaagaag
     3121 cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa aaattcgacc
     3181 aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt gcaatgaaac
     3241 ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag aaaacaaatg
     3301 aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgttccaga agaattgata
     3361 aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca ggtatataca
     3421 gataaaattg acattacccc tgaagagatc tatcaacgtt tgccagagga aatccctggt
     3481 acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt
     3541 tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata taatgtggtt
     3601 cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac gaaaaatatt
     3661 ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa
     3721 tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat
     3781 gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc ttctattgta
     3841 ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat ttattacaca
     3901 gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa
     3961 aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc gaaagaagaa
     4021 gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca aatctacttt
     4081 ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg tgtcggtgta
     4141 caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca gtgttttcct
     4201 tttttattga agttagaaca aaaaataata aagtattttg cactttcatt ttaattttct
     4261 cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg agaaggtatg
     4321 acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga tgatgagaag
     4381 ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgacgt ttatactgca
     4441 tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt gattttactt
     4501 gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg caaaacttat
     4561 gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt cctaggctta
     4621 gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt agttgcacgt
     4681 gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga ggcagcacct
     4741 gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt gacgaaacga
     4801 ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt ggcgaagcac
     4861 cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta tgattatttt
     4921 ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat
     4981 ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt gcgtaaccca
     5041 gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca attttaagat
     5101 cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg cctactttat
     5161 acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc aaacggtgga
     5221 atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag atgatgaaga
     5281 ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata tcatcgaaat
     5341 acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc
     5401 tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac gttatgttgc
     5461 tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg
     5521 cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat attcggaaat
     5581 caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta tttcaatgat
     5641 cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga gagaacaagc
     5701 cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac aagatgaact
     5761 tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag ctcaagatac
     5821 aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag acggcgtcat
     5881 tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt ccttgctaga
     5941 tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata tcgaagaaga
     6001 atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc gttcaaccag
     6061 tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc
     6121 aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag aaaaaacaga
     6181 acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac cgttgacaag
     6241 tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg aagtagcacc
     6301 aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga tcaatgacct
     6361 cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat atgtcaattt
     6421 caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa
     6481 aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag tagatacaga
     6541 taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt caccagatgg
     6601 gggaacgatt acttgtcgct tattagaaac gcataataac gtgattttaa gtatcaccga
     6661 tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt
     6721 agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta tttctagaga
     6781 agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa aagggtcgac
     6841 tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat gagaatctct
     6901 gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata cttctcttat
     6961 gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac aaacactcaa
     7021 atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct tcatgttaca
     7081 tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa
     7141 ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg agacaaatca
     7201 cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa tgcgcctttt
     7261 ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa cttggcaaaa
     7321 gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa aaaaatccga
     7381 tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa ttggacagat
     7441 atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac agaaccatcg
     7501 acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaagaagta tagttatatc
     7561 ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc agatagcgtg
     7621 aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac aatgtctatc
     7681 cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa agaaaatgct
     7741 agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt agggaacctc
     7801 agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga aggcttcccg
     7861 gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga aacgaatggt
     7921 tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat accttcagac
     7981 gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc taagggagca
     8041 gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg
     8101 aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa ctggtattca
     8161 gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga tttcaaaaaa
     8221 atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt cagcagttat
     8281 catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga atcaatccaa
     8341 caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga gaacaagcaa
     8401 ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa ttatcgcaag
     8461 gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa
     8521 tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat tagtagttcc
     8581 ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta tgtgtacacg
     8641 aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca agagtatgaa
     8701 ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa aaacgataat
     8761 gattatagca tcacgaaata catgcagacg catttatctg atatcgagca gctgcgcgaa
     8821 aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa taacaaaata
     8881 ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct gaaagtccgg
     8941 gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga taaagtggtc
     9001 caaattgaga gcgtcaacgc tttcagcaat acgatcgtga caaacaatac tgtaccaaaa
     9061 gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt
     9121 aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc aagtggaagt
     9181 tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt
     9241 agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga ccaattagat
     9301 ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt cttggctcgt
     9361 aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga accactgatt
     9421 ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag aacattcggc
     9481 gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca attttatcgt
     9541 ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg cagtgattat
     9601 atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca tgatcttgag
     9661 atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg agatagagga
     9721 catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa
     9781 cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc tcatttaaca
     9841 atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga aatctatgat
     9901 acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc aataaaaata
     9961 taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt atttttttag
    10021 catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct tttcatgttt
    10081 ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc
    10141 gctagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg tacgtttgcc
    10201 atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt tagattgttt
    10261 caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg catttttagg
    10321 tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag aggcaccccc
    10381 aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg acaaagaagt
    10441 ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa catcaaaaga
    10501 tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa catctgatgg
    10561 caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca atgcttcgtc
    10621 taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca taaaaaacct
    10681 ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt
    10741 tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag ttaaattaag
    10801 aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt ggttagatcg
    10861 tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg ctttgcgtaa
    10921 tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct tatttgctca
    10981 ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta tgtattttac
    11041 acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg ccattgcata
    11101 cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag ccttgtctgc
    11161 ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga caggtgctga
    11221 agggatacta ggagcagtta caactgggct catcatcgga tggggctatg cacgtttagt
    11281 aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt ggcagtcttt
    11341 tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt cagcggtttt
    11401 tcaacatttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc agcttcccat
    11461 tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg ttccgttttt
    11521 atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca
    11581 agcgaattca ttagcaaatg cagaaatctt agcctcagga caagagttga cggtggcaaa
    11641 tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta cgggtgctgg
    11701 attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga aatacagaga
    11761 attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa ttattttttc
    11821 cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac cggtcgtttc
    11881 aggattgatt acctattccg ctttatattt tggattcgtt cctttattta cagcagtcca
    11941 agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag tgcctgcagc
    12001 gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat ttttgaaaaa
    12061 aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag cagcagaaaa
    12121 gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa tcaccaatct
    12181 aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca gctaattgaa
    12241 caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag atccgaccag
    12301 ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc agatgaccat
    12361 cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa aaatttgaat
    12421 atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg atgggtccag
    12481 caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg atattttgtg
    12541 tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca gcgatgcttt
    12601 gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt ggagcagaag
    12661 ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa aaaatagcta
    12721 ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca gacgtagatt
    12781 ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca tagcaaaaaa
    12841 tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg gataacaagc
    12901 atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat aagcagctgt
    12961 cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag aatgagtatt
    13021 attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa aaaagagtta
    13081 tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa
    13141 gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa
    13201 actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga aaaagttcct
    13261 gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa
    13321 tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa
    13381 atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc atggcaaaag
    13441 aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat aaattagcag
    13501 atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa aaatcttacg
    13561 gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc
    13621 attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca aatgatattg
    13681 caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa
    13741 aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa
    13801 aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa gaagccattg
    13861 cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt gctgatgcaa
    13921 tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag
    13981 aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa tatatggtaa
    14041 cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc acagacaaaa
    14101 aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa caatcacgtc
    14161 cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt gtattgaaca
    14221 aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc gatcgtcgta
    14281 aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact gacgatctag
    14341 gtttagaatt gaaagatgca acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag
    14401 ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg
    14461 tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc gaaaaattac
    14521 aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt gcagctactg
    14581 aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct acacgtgccg
    14641 cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt atcagcaaag
    14701 tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt gttcgtgctt
    14761 tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca gtgattgttg
    14821 ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga gaatgggtaa
    14881 acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg
    14941 ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat aaaccagaac
    15001 cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg atgtaggaat
    15061 taaaagagaa cccttatatt acagggttct cttttctttt tttgttttat tttaagtaaa
    15121 aggggcaaaa aaggggcaga aatatcaaag cctatttagt ttatcaatta cttgttgttt
    15181 tgcttttttg gttacgtgat tgtaaataga cagagttgta tttgcatcag aatgtcctac
    15241 acgctccata atggctttaa gaggtacgcc taattcagat aatagcgaaa catggctatg
    15301 tctaaatata tgcgatgata gatttttttc taattccaac tcttcttcta ctttatggag
    15361 tacagcgtta aatgagtgca gcgtaagcgg agtgccattt gtagatataa atatatattg
    15421 atcgggattg gtgggtctac ctgcaagaat attgtcagct atcacacttt caatcaattc
    15481 ttttgcacga ttggataatt gtacttcacg ttgtgagtaa gtattttttg gagttgtttt
    15541 tatggcgtta tccattttta cagatgtgta atctaaggtc ccattaatgg aaattttacc
    15601 ctctccatag tccttcattt gcaaagccag caattcccca tatctcaaac cagtcaaata
    15661 tagaaattca gctattatgc cgtgtagttt tctgcgagga ttggaatata actgtttcag
    15721 tatttgatca atttcttctt tctccagata ttttttatcc atagaaagct ttcttttttc
    15781 ttcttccact tttttaggat ggattttaac tgctagagca gggtttcttt gaatgtattt
    15841 tctatcaatt gcatagttta gcataacgga tagagttgtt tttgtttgct ttgtgtagtt
    15901 cagtgagaga tcaccaaaag tgtacatatc ttcaatcatc ttattaatga gtgtctcatc
    15961 aatgtttcta acgattgtat catcgcttat gtgcttagaa acatgtttca tcatcattgg
    16021 aaccttcaaa tagctagtac gtttaacatg ctgcttataa tattcatacc attctttata
    16081 cagctcacca aaagtgatat ctgatttatt gtaattttcg agtgcttctt taattttttt
    16141 atctagaatt ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa gtgttgtaga
    16201 tttttttcgt gttttttctg tataaggatc tacatatctt tcaataaact tgaattggcc
    16261 atctttggtt tgttcaaccc acacttttaa catctcctat catttgctat aataggcata
    16321 acaaatagac ctatataggt ttgttttcta aaagcacgct cttactctgg acggtggggc
    16381 gtgtttttta tttattatac agttgcttta aaaatagctg ctttttcaaa tgatactaaa
    16441 ggagagaagt ggatttctat ttctcccttt tggtttaacg tgaaatgagc agttacatcc
    16501 atttgttttc ctggagcaac agatcccatt gtattttcgt tggcatatgt ctctgatttt
    16561 ttgtcatctg gtccatatac ttccacatct gtacctacag ggatatctga atcgccatca
    16621 ttttttacgg tataagtaat ttttactact tgtgcaggtt ggttttcttc aaattgattt
    16681 ctttcatcag ttagttctac actatttagt gtatattcag catcaccaac ttttacagtg
    16741 tcaccaatct tatagaaggt atcgcttttt tcttctttag aagatgaagc agaggttgaa
    16801 gatttagtta ctttttcgcc accattatca cttgctttat tactatctga tccaccattt
    16861 aaagcagaac caataattat aattaaaatt actgctaata cccaaaacca tacttttttg
    16921 taaaaaggtt tactaacttt atacattttt ccgtcttgac ccataacttt ttttgccatt
    16981 taaatattcc tcacttcttg ttataatata tttgcgatct cagaaatgag gtatgagtcc
    17041 gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga
    17101 tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca
    17161 gcataacaag tgtatctgag cttattctga atagaataat attccataaa tttttcgagg
    17221 ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata
    17281 gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa
    17341 actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct
    17401 tctgcagttt cgccatatag attttcattc aaaaaaatga ttctaggctt cggataatag
    17461 aagccaggct ctttcatttc catgtaaact actttcaaat tgtattcgct caacatttct
    17521 ttcaatttca aatacataca acccatcact ccaactattc attttcctct aaagctttag
    17581 cgattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa
    17641 cagtatcgtt ttctgataaa tccacatgtt taggggtttc tttttcttcg cgccccagaa
    17701 gatagtctac agagacatcg aaatagtcag caatttcttg caaacgttct gtagaggggt
    17761 ttgagttctt aagtctatat aagacgtttt taccatagcc taggtcttct tcaactttat
    17821 ttaatgattt tccctgtttt ttcgctaact cttttattct ctcaaacgtt atcatatcaa
    17881 tattcctttc gttcaagaaa aaatatttaa ctttatttgt taaaattcgt tgactatttt
    17941 aacaaaaggt gttaatatca ttcttgtaaa caagtttaac aactaaaaag acaacaaaaa
    18001 acactattga ttaataaatg ccaaccgcca agaaagcttt taaaatcaat gtttatatgt
    18061 cttatttaac tatgttctga ttttaacact gtatgttaat ttagtcaacg cagtttttta
    18121 aaaagttgtt aaatttgttt acgaatataa aagaaaggag agaagaatat ggaaaaaaca
    18181 gtctcggcaa aaatcgaaga tttgaaatta gacatactga aacaagcaaa agtggcgatg
    18241 gaacacgcgg tagataaaga agactctgcc atggttgcag ccatagcaga gattttagct
    18301 cacgtttagt catcgttttt acggtcttct ggaagctgac catagataac agcataatga
    18361 atatctaaat aagtttgaac aatttctttt ggcgaaattg attcgccttt agtagatact
    18421 tgtgattcgt gatttagcca tgcaacaact aaatccgcag cgatttcaaa cggcgttaca
    18481 ctttgtgtca gttgccataa agaatttcac aatatgtatg gtcgtggtaa taataccaaa
    18541 gagcaatttg aggaatttat ggaaattaaa aaatacaaaa aatcatttat taaaggaggc
    18601 taaacaatga acaaggaaat gagccaaaca atcaaggtac aaaaaatgat agatgatcta
    18661 acacatggaa ttgatagcca agccgataaa atcatcaagg aattgcaagg acaaaaagtt
    18721 aaggatgcaa aaatgcttct caagactatc aactttgaaa tgaatccaac aaaaagaaag
    18781 ctcgccgatg tgttggaaga aaagttggct tccgctatca acgagcaaga attactattt
    18841 gaaaccgata cttttaacag ctgatgtttt atagatgaca gtttcatccg aatctatatc
    18901 atcgccgaga gaaaagaatg ttgcttttga caaaaagccg ataagaccta caagcggtct
    18961 tgatgttgca agagtagata ccccaatatc accttgcaag gtattcacgt aaatttgaga
    19021 agaatagaac attccatctt tttcttcgat atttgacgca tcatcgtatc caacgaccaa
    19081 agtatcggca aatattttga ttttttctcc atcaagcata gtaattacaa attctggttt
    19141 cataaatttt cacctcctta acaattattt cagcctgtca cactgataag gaaattatac
    19201 caaagaaagg aatgagaaat atgaatacac cacaaatttt taatttcgag caaaacgaag
    19261 ttcgaactat tttagtaaat gatgaaccat attttgtagg aaaagatgta gcagacgttc
    19321 taggatattc gaatccgcaa aaagctattc gagatcatgt ggacttggag gataagacgc
    19381 agaacgattc gttcaccgtc aatggaacag cagttgtttt gattaacgag tcaggccttt
    19441 acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacga
    19501 gtgaagtcct tccaacaatt agaaaaacag gtagctattc aaacgtacct caaagttttg
    19561 cacaagcatt gcgtttagca gcggatttag aagaaaagaa ccaattactc gaacaacaaa
    19621 ttgccgagta cgaaccaaag attagctact tagatacgat tctttcatcg acagatacgg
    19681 tagctacttc tcaaattgca gctgattacg gaatgtcggc aattgctcta aacaaattgc
    19741 taaacgagtt aggtgttcag cataaagtta gcggacaatg gatactttac cgaaaacata
    19801 tgaaccaagg atacacaaaa tcgcacacaa gtgagatacc gaaagccgat ggcggcacta
    19861 aagttgtaat gaataccaaa tggacacaga aagggcgagt gtttatttac aacttattaa
    19921 tcgcagaggg ctattaccct caaatggatt tagaggaaat tggttagaaa ggagttttag
    19981 tatgactgac attgcagaaa tcactcaacg agatagagaa aaaatcaaag aatatgtcga
    20041 aagttcaaag ttcttaactt acaccatgct tgctgaaaga tttggaatta gcaaaagcta
    20101 cttatcttta attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc
    20161 gattatcact atgtacgaat tgtagaggga ggtaaagtcg ttggaagata aaatcattct
    20221 tacaaaatca gagcttcaag aaatgttaaa catcgagtat ggaacgataa tttttaggag
    20281 ggataataag tgaaaaaacc aacactttca gagctgatag aagctgctga gaaggcagta
    20341 aaaccagacg actggtaccg acaaagttta atcttggaga agttccacgg catgtcaaaa
    20401 actactttag ttgaatactg caaggaaatg gaaacaattc ctgaatttgc agaaggaata
    20461 cttcgtccag gtcactcaac cacatttatt cattaccata cttttatttg gtttttaaag
    20521 tggaaagacg caaacaaata tcgtgtaaaa agtttatcgc cttcagatgt tttgaaggag
    20581 gcaagttaat gggcaaattc aacagagcac tagtattcag cgcaccgcta atcatctacg
    20641 ctttaggcct ctggggaagc agacaagcgt tgataggtac gattgtgtac atggtttgga
    20701 tttttatagg gctggatgaa gctgagtaca aaacaaaaaa gccagtcggg agggactgac
    20761 taaaaaagtc taatgtctat aagaaaggtt tgacaataaa tgaaagaatt atttttttta
    20821 ataactttca tcattttttc tatcagtgtt ttctacatta acctttgcgt tgctagattt
    20881 gctttgaaaa gttttaggaa cagcaaacga aaatagagct ataaaagata cgaatgtagt
    20941 agctacgtta aattgctctt tagaaaacgt caatgtcatt ggaatgataa ttgatatcga
    21001 tgagaaaatc tttgcgataa taactatcca tttttcaatt ataaacattt ttaaattaag
    21061 caccattaga caaaatgtag ttcctatata gaggataaat gaagatagaa ataaacatat
    21121 tagtccagtt ttaaaaaaag agaagtaatc tatgtgggca aattctgtta atatgctttt
    21181 ttgatttaac acaatcaaac tactgttgga caaaaatgac agtaaggaaa gagttgctcc
    21241 gattactaaa taaaaaatag ttgttaacaa tattatatta tcaatttttc tgctcatgtt
    21301 ttcaccccac cagaattata tcaaaaagga gagaagaaat aatgcaagaa ttagtaattt
    21361 tgaaaaataa agaagctgtg actacgagct tacaagttgc agaaagcttc gataaaaagc
    21421 acagacatgt tttgacggct attgatgaac taaaagaggg ggttgccgaa aattgggcag
    21481 acctattttg ggaagatact tatgttcatc ctcaaaataa acaatcatac cgaattattt
    21541 atatgaatag agacggcttc tctctattag caatgggctt cactggtaag aaagctttaa
    21601 gttttaaact tcaatatatt gaggccttca acaagatgga aaaagaatta aaagaccaat
    21661 tgccatcaat tccagcaact aaacgagaaa tggcattact tgctctagct gcaaacgaag
    21721 aaacgaatga aagagtagac gctatcgagt ctgatttaaa cgatttgaag aacaatcaac
    21781 ttctagctga accagattac cgaacaattt ctaatatggt tcgcaacaaa attagagtta
    21841 tttgtaatca gcagcattta aatagcaaag caaaagcaga attgtttaaa gacttgaatg
    21901 gcggaattaa gcgaatcact ggagcagtag ctagaaatcg tatcaaggca aaacagttcg
    21961 atgatgttat cgattttatt aacaattgga tgccttctac agcgacgatg acaattatta
    22021 agcaaatgga gttgttagag gatgaataga gctgaagcgc taaaaatagg cgtaataatt
    22081 gctaatcgct ggtggagaca caataaacca aacatcctaa gccaacaaca tattgataag
    22141 caaaaagcat ggcaacaaat aaaaaagtga ctcagccgac caaagcaatg agtcacaaag
    22201 aaaatacatc taaggagatg ttaccacatg aaaaaagaac tttccactct agatcaatat
    22261 ttgactgatc ctagttgggg caaatcgaat atcaaggaaa caagcaatcg aaaaatcaga
    22321 cgtaatcttt tgacggatga agaactagca tgtgatcaag acgatttggg aaattttgtg
    22381 agtatttggg atcatgtcta tcttatccat ttatcgagga agtccaaaaa aactgaatat
    22441 atctatgtca tcgaagatgg cttgattgat gcgctagaag agtatgacag agataacttg
    22501 attgatatct cttattacgg atcaggtaag aaatacattg ctgaaatgga ggcagaattt
    22561 gatgagtgaa aaggaacaac ctttgaaaaa tagaagtgat aatacgcttt ttaatacctt
    22621 atacaaaata aatgtgaaag atgttactga aaaacgaaat aacctcactt atctttcgtg
    22681 ggcatgggct tgggcagaag ttagcaaagt gtgtgaggca gtagattatg aaatctatca
    22741 tgatccagaa acatatctgc cgtatgtctt tgataagaaa acaggctata tggtttttac
    22801 cagtatcaca gtcaacggag taaagcgtga catgtggtta ccagtcatgg atggtgcaaa
    22861 caaggcaatg aaagatgagc catataccta cgaagtcaat gattatcagt ggaataacga
    22921 aacgaagaaa aaagagattg ttggaaaaat cgaaaagcga gttgaagcag caactatgtt
    22981 tgatatcaac aaaacgatca tgcgctgtct tgtaaaaaat ctagcaatgt ttgggctagg
    23041 gctatatata tttgctggcg aagatatgcc agaagatgtc tcgatgcttg aaccagctac
    23101 tcaaagaagc aaaaagctat tcttggatgc tttacaactg gttgctaaca agtacgaaaa
    23161 atcaattgat gaagcaattg ttgcattgac tgatgcggct tctataaccg ctgatgacag
    23221 taaatggacc aagagagact tgggcattct aaaacgaggc gttaactgga ttgaagatca
    23281 gtacagagaa gaaacaaaag agaagtgata tgagtgttta aaccattgat cgattcatat
    23341 tcagcggttc tgaaaaagtt caaaggaaaa gacataggtg caacgatcaa tgaagaagtg
    23401 aacattgatc gactaaagac gatgtatgac ggatatgatg gcgatcggat tattgaagtt
    23461 cgatttattg atccacgtcg atttactgtg cagcaacgaa acttcatcta tgcgctgata
    23521 ggcgatattt tcatcgatac aggcatgcca acggacttct ggaaggaatt cttttacttc
    23581 cgttttgaag gcgtcacagg gcgcaaaata agcctcaaag acgaatcgaa tacaactgtg
    23641 agtgatgcta atgtcttagc aaatatcatc ttagatttca tctttgaaca tcatattcct
    23701 ttcaaagaag gctatgagat tttaccagcg aatcaagaat attacttcta caaatgcatc
    23761 acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca ctttgacaaa
    23821 gcgctaggaa gacgaaagcg caaagaagtt gatcattcag agtacacatt tgcagcactc
    23881 tgcaggattc atcacacgga gaaacacaat ataggtgtga tcaatttcaa aaataaatat
    23941 caaatcaaag ggatcaagtt aaaccaggaa acaattaaga aacttagaat aggagggtaa
    24001 aaaatattgt ctgacaacaa acgctactac tatttaaaac taaaagagaa ttttttcgat
    24061 agtgacgaga tggttctctt agaaagtatg ccagatggct atatttattc taatattctt
    24121 ctcaaacttt atttaagaag tctaaaacac gaaggtaagt tgatgtttaa tgacaggatt
    24181 ccatttaact ctacaatgct cgcaactatt acaagacatt ctgtaggagt cgtagaaaaa
    24241 gcggtacaaa ttttccgtga tttgcagctt attgacgtat tagataacgg agcaatttat
    24301 atgtctgata tacaaagttt cattggaaaa tcctcaactg aagcagatag aaaaagagaa
    24361 tacagaaaga aaatagaaga ggcaaaacgg aatttaataa ctggaggaca agtgtcggac
    24421 aaatgtccag acaaaactac accagagtta gagatagaga tagagaaaga tatagatata
    24481 gataaagaag aaaagaaagg taagtattct gacgaacact tacgccttgc taaaaaattg
    24541 caaagtaatt taactgaaga ttttccaaaa gaaatgaaca aagtagatat cgaaaaatgg
    24601 gcagacacaa tcaggttgat ggaagaaaga gataaagcgt ctatagaagc gattgagtat
    24661 gtgatcaatt ggctacctac aaatgaattt tggtttggaa atattagaag tgctaagaaa
    24721 ttgagagaaa aatttgagaa gctcaaattc gaaatcaaag cagacaagaa taatcataaa
    24781 aagcaaagtc aaaaactaca gtacagcaat cctagtgaat atgacgactt gccaatttaa
    24841 aaaggagatg catcacatgg aaagcctagc aaatgctatg gagaaactaa taagaagagt
    24901 attagtgcaa agcgggaaat gtccagaatg tagcgaacct ttgtatagtt ggcgagctaa
    24961 aaataaggat ggttcagaac gttgtaaacc aacatgcatg agttgtggtt ataaagcgtt
    25021 acgtgtgaaa gaggatatac agaccgaacg gatatataac gacagcttaa aagcacgagc
    25081 gttgagtttt tttcaaaatg gttcggtatt aacagataaa actttgttta aatgcaaaat
    25141 ggagaattat cacgtagtgg accaagaaac gaaaattgct ttagaaagag ctaaaagctt
    25201 tgtaaatgat gtcttactga accatcctgc acatttcatt ctatcaggga aatcaggaag
    25261 cggaaaaagc cacttgtcaa tggcgacagc ttgggaaata cttgagcgct caaattatga
    25321 caagaaaata ctttttataa gctatcaaga gttattagag caaataaagt tttcttataa
    25381 caatgctgaa ctgagaaaag aaattgaagg atcgcttata gccgatatta aaacaactga
    25441 tttggtggtt tttgacgata ttggagctga attaggtagt ggggtatcaa atagtaggca
    25501 gtttacaaac aacacgttaa acacgctctt agaagcaaga cagaacaagg caacgatcat
    25561 cacaacaaac ttatctggtc ctgaactaag agaagcctac ggtgaaagaa ttgtttctag
    25621 gatatttaag aattcagaag gttatgcgct gaaattccag caaacagcag acaagcgcat
    25681 aaaaccagtg aaaggtagta tcgcatgaat aaataccgta ataaaaaaac tgttcatcga
    25741 ggtatcaagt ttgattctat cgcagaagca gagtattacg atctagcctt gtggcaagct
    25801 gaagcgaatg gctggaaagt aaaacttcag gaaagatttg agctgatgcc gaaatttgaa
    25861 ctagacggaa agaagtatcg caagatcgag tatattcccg acttcacatt ttataaaaac
    25921 ggcaaacttg tcaaagtcgt agatgttaaa ggaatgcaga caaaagactt taagatcaag
    25981 gcaaagctgt tttgtcatca atatcaagtg ccgttgatat tagctaaaaa atatcggaat
    26041 acgttcaagg aagagcgttt ttaaagaggt ggtctatcat gacaacagaa gaagtgattc
    26101 aaatgcgaat tcgaagcatt caacgtgaga ttgacgatct ggaacgaaca aaggcagtga
    26161 tggtcaatga aacggcgaga aaggcaatcg atttgcacat agagaactta agaagggaaa
    26221 ttcgtagatt ggaggaatga gcgtggataa gaaagcagca atgaaacgaa tcatcgaact
    26281 gacacattct gagaattggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg
    26341 taaatcaatg tggactgaaa aaaccagacg gagaatgccg agaaaaattg caatctggca
    26401 cggtgaccga attctagtaa caggtaccgc tgaacagtta tctgaaatta ctggtctgag
    26461 caaaaacatc atctgggata gagcgaagaa tatggatatt gattctaaag gaagacagtt
    26521 taggtatgtg gaggagaaat aatgaatctc attacacaat acagtgatat catcctcaag
    26581 aaaatcatga tgaagattca gaaagacaaa aaatcaaaag aacgagcgga attagttaag
    26641 ttggaaatgg ctgaaacagg ggcaggagtg cgaagtagca ggcattggaa ggcagcagca
    26701 aacattgaat tttattacaa cgaaattcaa aaagggttcg atcaaatgcg tgagctggat
    26761 cggcaaacaa attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaaatat
    26821 aaagagatat tagacgaata catggagggc agcaaatgat accgagattt cgatcgtggt
    26881 acacaccatt taaaggtaaa acaattggac aagaaatgaa atatgggcaa gcaggaaggt
    26941 tgatcactca tgctgaaatg gctccagata aatatgtgct tatgcaatcc acaggactga
    27001 aagataagaa tggcgtagaa atatttgaag gggatgtagt tctggttaat gtaagtaatg
    27061 gttttgatca tttagttaat gaaaaaacgg ttgtacaaga atccgagttt cattccggat
    27121 tagtttgtaa atcattaatt agtggaatgg aatatagaat attcagccac caaaagatgg
    27181 gatacgaata tgaagtcacc ggaaatatac acgagaatag cgagttattg gaggtagagt
    27241 gattatctta gctactgact acatcgattc tttaaaagat gaaaatggga atgtcccagc
    27301 attgctaaat tgtggaatta aaagtaagaa tagaatcatt ttgttacgga tatcggctga
    27361 tttagcaaaa tatctttatc tgagcaagac agcagtatat tacactgcgg ctacacgtta
    27421 tcagtacaag ggaaaaacaa tcagtcagga ttattatgat cgtttaatca gtcctgacat
    27481 acatggaaaa tcaaaatcag cgatcaagtt atttggcgca atagagatat atgccgatga
    27541 ttttccaaat ttatggttga aggaggaaca gtaatgaaag actggttaga agaagcaagc
    27601 gccttagttg gctgtttcat aaactgtgcg tttatatctg taattattgc actggtggga
    27661 tttgtatcat tgaaagtgat tatattgctt tggcagttaa ttttttagga ggaacagcga
    27721 tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agtgatagga aatgatgcac
    27781 tagctgcttg ctataacgag agtataaatg caggtattac gttaatgaaa aaaatagacg
    27841 aaccgaagaa gctagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc
    27901 cttttacgaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt
    27961 atttctgtga ttggtttgta cgagatggca tagtgacgca agaacaagga gaagaattac
    28021 tcgcttgggc aacgagacaa tcatatgaaa cactattgag cttgtacaac ggctacgagg
    28081 tcgagaaaga gccgttgtac agtgtaataa ttgctggcga gtaccttgtc aaggagatca
    28141 gttgcagtaa tgaagttaaa ctagtatcaa cagcttacct gccacaatac cttgcacacc
    28201 actaccaact gaccgaaaaa cagatcaaag caattgatga aagatactgg ccatttgctg
    28261 tgccagtgga agaggtggcg gaaggatgag tagacagata atgctccata taccagacgg
    28321 aacaaaggca atgacgatta ccattgttgc tgcacaaaaa gacggtgggt tggcgttgaa
    28381 aacaaaaagc attgatacca aacagatttt agaaggcaga gatgtcgaga tagagatcga
    28441 tgaggaggaa gcggaatgaa tgttcaagtt tatttaaaaa gagatttggc caggtttcct
    28501 cgaagctggg atacaaattc ccacccatcc gaggcagaag aagagggcta tctaatgtct
    28561 gctaacatga tagaaattac aagagatgat gcggatgagt ttgataaaaa aagatcatgg
    28621 tactcggttc cgaatccaat gtatacggct gtaatagaag attatcatat atccgacaga
    28681 tttattctga ttgatctaga aaggcctaag aaacgaatta aacagtatag acgttgtgga
    28741 ttttcaacaa agaaatggtg ataacaaaac agaggtgaag aaaaatgaac aacagacacc
    28801 gcagagtagc aaaactaaga aatcaagaac tgaatgtagc gaaagtaaag ttcgaacggg
    28861 agtatggtgt ttctgcagaa gaaacatata aagtggcaag tcagtgtgtt gctgatgcaa
    28921 gtgatgctat tcgtaagttt ggaatttcga tactagatat taaatgggag gatacagaat
    28981 gaagatttat gtagtaaagt ttggaaatca attttacaga agcgatgaac gttctatagg
    29041 ggctaacaca ttatccattg tagacatact ccaaagcgca agatggtttg ataatctcga
    29101 agaagctaac caagtttcac gacgacttgg tggattaacg caagtatatg aactggtcac
    29161 tgtcgatcat gagggggtag agtaatgaaa ctaaaagacg gattttacgc tagtagtcat
    29221 ggtatcggcg gtttgatgct agatatgccg acaaagaacc ctaaaacacg tgagaaacca
    29281 aaattcaaag tcggtgacat ggttcgctgt gaagcagaag gatttatcta tccatttcgt
    29341 ggatatgtag aaaaggtatt gtcgaactca gcaatcattc gtattgaaaa cacgatggaa
    29401 tgtgacaagt ggttagcgaa aagcaaagag aatttagctg tagcgagatt agttgacatg
    29461 gaagtaatcg agagcaaata aaaaagccgg atcgctccga ctaacataat aaaacagaca
    29521 agtttattat atcacataaa ggagcggttt gacttgatgc aattgttacg agaggtagat
    29581 ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga actttcggcg tttggagcgg
    29641 atggcaggtc gctctttgat agatattaag tctccaatta ttacggatat gccgaaggcg
    29701 ccgaagcacg gcaataaggc agaggacgcg atcattcaga tgatggatat agaagcagag
    29761 agagatgcga ttttagcggc tttgatggct cttagtctga ttagccgtca gatactctac
    29821 tacagctttt gtgtgccaga tagcttctca aactacagaa ttagccgtga agtgggttat
    29881 tcagaaagaa gtatacaacg gatgaagtcg gaagctctaa tagagtttgc agaagcatat
    29941 aaacacggaa gaataattgc ttataaataa tttggcggtt ttttggcgga atgatggcgg
    30001 tttttagcta tttaccagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    30061 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    30121 agcaacggaa atattaagaa taaggatgtg aatttcaact ccttctaaat tgttcttatt
    30181 atctatcatc cgttgctgtc tattgttttt ttaattattc acacgataac taaaggtgag
    30241 tgaagagaaa tgattccatt aataatttca atttttgcgc tctgtcttaa tgtctatatg
    30301 attggattta aaaatgggca aaataaaaaa tagtagcagc caagaataat tttatagtgt
    30361 cactgtggcg gaaagggtag acgcttaaaa ataaggtcaa tacgtcgagg gatagcctta
    30421 acgttttatg atttgaccat gcaatgttcg attcattgcc agtgactttg gcaaccgagg
    30481 gttggaaatg ggcgctcaaa gtacacgagc aaggcgaggt cgatagtaat cgatggaatc
    30541 ggtgtaggtt gcttgataga gctataactg catctcgttt atgaggtgta gtttttacag
    30601 attagatcac tctttgagtg gtctttttat tttacacaaa ggaggtaaca agaatgtata
    30661 gaccacaata cttagaacag aagtatgaag taatcactgt gcaaaacggt aacggtgaga
    30721 tagtacgaaa gtatagaaga ccaataaaga gcgatacata caaacgaaag gaaagcaatg
    30781 aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg cgaaaggaga
    30841 cggaatatga ccgaggaatt ctatagatgg ctattacagt tgataagaga agatcgttta
    30901 gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat gaaacgagat
    30961 cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga aaacgttcat
    31021 catataaagg aagtcaagga tagacctgac ttagctttag atttagataa tcttatttgt
    31081 ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt agataaacaa
    31141 gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta agtgcatgat
    31201 catcaatgat aatggcagag agtatgatac agaaaagatt gaagagtatt catcttatac
    31261 tcagggatta attaaacgtt tgatatacgt tcgctatgta gatattaggg atctgttatc
    31321 agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga ataaagataa
    31381 taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta attattacat
    31441 tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa taaatcgcaa
    31501 attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt tcgaacttta
    31561 tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa
    31621 attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg agccattctt
    31681 agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg aagaagatat
    31741 atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag cgttgcttgt
    31801 ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc ctgaattggt
    31861 aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta
    31921 tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat tatctagaag
    31981 aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag gattcaatca
    32041 atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt ctacaatgtt
    32101 ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga tatctttcat
    32161 taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc tttctactaa
    32221 ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat gaaagccaag
    32281 caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat ttagataaaa
    32341 aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc aaacttcgtt
    32401 ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg
    32461 aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt ggtggtaaaa
    32521 ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg
    32581 atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt gacaaggatg
    32641 gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat aatgaagttg
    32701 atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca aatttaaaac
    32761 ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga cttacgttca
    32821 tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct tcatatgatg
    32881 atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag
    32941 tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa tacgataaag
    33001 atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta caaaacatca
    33061 aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt catggaaaaa
    33121 atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa tattatatta
    33181 aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta gaagaagcag
    33241 gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta aaagatctgg
    33301 ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt atgcgttggt
    33361 attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat gaaaaaatag
    33421 aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg aattttttag
    33481 atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa aatacaggaa
    33541 ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg gatttttcaa
    33601 aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata cttttgtaaa
    33661 ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga tttgattgca
    33721 aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa aaagaatgat
    33781 ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga atttagaaaa
    33841 aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc gtctaataat
    33901 tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt gaaagatgat
    33961 gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct agaaagtgat
    34021 gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc gtattatcaa
    34081 gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa cgctcgtaga
    34141 tacgtactga aagggaattt attccgaccg caagacaata caacacaaga tcaaatcaat
    34201 aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg tgcggtattt
    34261 caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag caattcaaga
    34321 gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt tcacgttccg
    34381 aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc ttttttaatg
    34441 ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag tctctatgaa
    34501 gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat caagattact
    34561 agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt atttacaatc
    34621 aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc agatgaaata
    34681 tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa
    34741 aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc agggatttat
    34801 tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata ttttggatag
    34861 tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag acgtatttca
    34921 agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg ttgtgattaa
    34981 cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta taaaaatcta
    35041 caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga
    35101 cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg cttcatataa
    35161 aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag aatcgtttat
    35221 ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg cagaaaatag
    35281 ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg acgttaaaga
    35341 aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat ttggacaagg
    35401 agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag cgaccaattg
    35461 atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt aagcttatct
    35521 aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga tcaattagta
    35581 ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta taacgaagta
    35641 gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt atttgaacgt
    35701 gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt tgttaacaca
    35761 actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt
    35821 tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat gaagcagttc
    35881 aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc agtatggtta
    35941 gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga acaagcaatt
    36001 gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag caaacaaact
    36061 agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc tgtaactatg
    36121 ggccgtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga tccgatttat
    36181 cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta ctggtctaaa
    36241 gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt attgccagta
    36301 ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat tggggtagcc
    36361 tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga tgaataccat
    36421 tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca acctaaacgt
    36481 aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac aactacaaaa
    36541 ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta tattctttgt
    36601 cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa tctgaagaaa
    36661 ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat tcctattttt
    36721 tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga cgacaaagag
    36781 tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga tcattcgcgt
    36841 gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag aattaatttg
    36901 cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag ttatctaagt
    36961 cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat ggcaaacatt
    37021 gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg cgcacagtgg
    37081 gttattaacc gtcctaaagc gaattattgg aaaaaggttt atctggattc taatcggcta
    37141 tatcgctacc ttagagggca aaaaacaaat atacaaattt ggacagccga gatgtgggca
    37201 cggctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga attagacttt
    37261 tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca caatgctgga
    37321 gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc tacgcctttt
    37381 gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt taaagcaatt
    37441 aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata gctgtgaagg
    37501 ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg atagctaaaa
    37561 atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct gaaagcttag
    37621 aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta gatgagtttg
    37681 aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta caagaatata
    37741 ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa cgatggttgg
    37801 ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat tggtgtagaa
    37861 gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag tgatataaca
    37921 gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt tcatccaatc
    37981 gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt
    38041 atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt gggtgaatat
    38101 ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg ctatcaaaac
    38161 ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg aggatgagct
    38221 acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga tcactgagcc
    38281 aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata gagaagattt
    38341 aacaggagac tcactagata ttatttcatt gattcaaaat cgttcgattc gttttcagag
    38401 aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc aattggtatt
    38461 tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg gagctaaaaa
    38521 taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac
    38581 tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg attacagaaa
    38641 aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa aatagtcgtc
    38701 cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga aaatttgagt
    38761 atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg gacgaattta
    38821 tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt cgtcaattcg
    38881 ataaattaaa taaatagggg gaacaacaat ggctaaaaca aaaactgtag taacaacgtt
    38941 cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa atgcaatcgc
    39001 aacgaattgt aatggacaat tagatggcga aacagaaatg caaacggtgg ttaaaaaatg
    39061 tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa caattactgc
    39121 acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg gacgtattaa
    39181 accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga
    39241 gatcgtggat gacttcgaag aaaatagaaa gttagttggt atgttagcat gcacttcgaa
    39301 tacaggatta acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga
    39361 aacaaaagtt atgcaagatg aatttggtaa attttatcat gaagcaattg ttgcagaact
    39421 tgaagaagac ttaacagatc aatggatgac gaatctatct gctgatgtga ttaaaaaaac
    39481 ttcagttgtg acaactacgg ccactcaatc acagtaaaaa aaacggaggt agcgaaatga
    39541 acgaagatta ctcaaaaatt gaactaaacg atggaacaat tttgaattta gaacctaaac
    39601 tgaatatcaa gaaattattg atgatcaata gagattttaa cacagacgag tttgcaaaaa
    39661 tgactgtggg aaaaggatcc atggatattt ctgttattca aggtgcaaag gctgtgtata
    39721 ttgcttaccg ccaagcgaac atgactgatt atatttcatt cgatgaattt atcgataaat
    39781 gggattttga tatggctact gccagctata tttatcaatt gatgatgttc aaacaagcac
    39841 gcgatgctta tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt caaaagtaaa
    39901 aatgccaaag ctcttagttg aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt
    39961 ttctatgcct tcagatttgg ttttaagcga tatctgttta gatgacattt tgcaaatggc
    40021 ttacaacaag agtgcttatg aaggatggaa aaactatgca ataaaccaat ctcagaaaaa
    40081 ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga agcagaagta actttcatag
    40141 ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta actaaaaata
    40201 gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa tcagacaagt
    40261 taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagccaa aaagttgaag
    40321 taactagcca acgtttagcc aatgccaaaa aatattatgg agaaaattcc accgaagttc
    40381 agaaacttga gagagaactg attaaccaac aaacagcgca acaacgtttg tcaaacgaaa
    40441 ttgataaaac gagtaataca ctagctcaag caaaaggcga aatacagacg tacgagtcta
    40501 caatgcaaca gttggatagt gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat
    40561 ccgaatacaa aaaatggcaa gtaactgctg gtcaatcagc ttctgaatcc gagaaattag
    40621 cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc agagaaaacg atagatatcc
    40681 tgagacgaca gttagaagct acacagtctg agtttggcgc tacatccaca gaagcaatgc
    40741 agatggaggc gaagcttaat gatgctgaac gtgaatttga agagttagga caagctgcta
    40801 aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg aataatttaa
    40861 tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta gggaaacaag
    40921 cagtggactc tgctaacagt gtaggtagtt cccagagtaa aatacaagct aattttggtt
    40981 tgactaaaca agaggctgaa gaattaacga atgtagccag agacatttat tataaaggtt
    41041 ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt aatttaggcg
    41101 atttaaataa tcaagattta caaaatatta cggaacaagc tatggtccta gaaaacacca
    41161 tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc aacttcggat
    41221 tgagcgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt ttagataaaa
    41281 cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa atgggatatt
    41341 cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct tataaccttg
    41401 ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt cgatttgagc
    41461 aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg aaaaatggtg
    41521 gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat atggatggtc
    41581 aatacgacca attaaataaa gcttctacaa tttggtctgc acttggcgaa gataacgcga
    41641 tgaaagttgt ccaatctttg actgatgtta accatacatt tgatgatgtt agtggatctg
    41701 cacaaaaaat gaatgaagat tctactactc cgttacaaga attaaacgga aaaatagctg
    41761 aattaaagga ttcattagct cctataggca acacaatcat agatgcactc gaaccagtaa
    41821 ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca gtacaggatt
    41881 atgccgtagc gattggcgga ttgactgctg catttacttt attaatgcca ataatagttg
    41941 gcttcatggc tctaggtggt cctactacat taataatagg agcagttatt actgctattg
    42001 ctggagttat agcaattata aaaaactggg gcgcaattac tgactggttt aagggaatat
    42061 ggagtaaatt cactgattgg ttgggtggta cttgggaaag cataaaagaa ggtgcctcat
    42121 cagtttggga tggagtcaaa gaaacctggt ctggatttgt agattgggtt caagaaattt
    42181 ggcaaggagt ttctgattgg tttggagagt tatggagcgg attagttgaa ggagcttcca
    42241 acatctggca aggagtccaa gagacttggc aagcattcgt tgattgggtt tcaaatattt
    42301 ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga attgttcaaa
    42361 taccatggac attaataacg tcattgattc aagctggtat taatattatc gtgggtatct
    42421 ttgatgtagc tggacagtta ttaggcgcag cttggcaagc tgtttggaca cctatttctg
    42481 atttccttaa aaatacttgg gatactatga cacaatggat aagcatcgct tggaatggaa
    42541 ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat ggtatatgga
    42601 catctattag tactacggct tcaaatattt ggaactcaat tagtgcaaca gcttctagta
    42661 tttggaatag tatcaagaat acaatcacta gcttggtaca agcagctgct acagtaattc
    42721 aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca atcagctcta
    42781 cagcatcaaa tatctggagt agcgtgacta gtagtataag caatgctata aacgcagcta
    42841 aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gatcagcgga atttggaacg
    42901 gtatcaaaaa cactgctttg aatctttgga atggaattac aagcactatt agctctaaag
    42961 taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt attgtttccg
    43021 acatattcaa taatgttaaa agcacgatag ctaatatatg ggaaggtatc aaaaagactg
    43081 ttagcgctcc gattgattgg attagagata aaatcagtag tgtctttgat aatttgaata
    43141 tttctatacc acatattccg ttaccacatt ttaaattgag cggagaattc aatccattga
    43201 aggggaaaat cccaacattg ggtgttgatt ggtatgcgaa aggtagtgtg tttaattctc
    43261 caaatattat cggtgtaggt gaatcaggac ctgaagcagt tttacctttg aaaagatctg
    43321 tgctgcaaga aattggtgat cgtatcttga gtagcacctc agtttcatct aggacacaag
    43381 cggttcaacc tgtgaacaat tacgaattca atttcacaat tgatggtaac gcagatgaag
    43441 tcactatgaa gcaaacaact caacaaatca ttgatagcat tacaaaagtt caaaatgata
    43501 atgcttcggc atggcgttaa acaggagagt atttctcctg tttttttagt attaaaaagg
    43561 atgtgaaaaa atgactgatt gtatacattc tataatcgat ggatttcctg attatttgca
    43621 taaattggct ttagctgaaa gaccaaccat accttctcca aaaagacaga gagttgaaac
    43681 ttctgtttta ggaaggttag gtggcttagt gcaagattac tcgtttgaag acatgtcgtt
    43741 tacattgcac tataactatt tagaggatgt ggaagaccat caagcgttca agcaatcgtt
    43801 ttatatcatg cgtcattggt taaactatgc aaagaaatta gaattctctg acgatcccaa
    43861 cgtctattac gttatccaga ctatcgatat tggggatgca gaaaacgata ttgttgaatg
    43921 gggagagttc gatgtaaata ttactgcgaa accattcgca agagttcaag aagatgtacc
    43981 tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag aagaaagttt
    44041 tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa atgattatgt
    44101 gtttagtttc gaaggcttag tagcaggaac tgatgtagtc attgatagtg atttgatgct
    44161 ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa tgaagaccat
    44221 gcaatatccg acattgcaag tggatatcaa tcattttaat tgcactggtt tgagcaaaat
    44281 acaaatttat cgtaacgggt taaggtaggt gaaatagatg atcgataatt taataactat
    44341 ttacgataaa aatgacgcga ataatttagc tgaacattta tatgatacgc aaggtttagg
    44401 cgctttgtca gactggttaa cagctactgt tagcaataaa ctaaacggag ccgagatatt
    44461 tcagggtact tatccaataa gcggaactaa tgcagatttg attgtagaag gacgtattat
    44521 tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt atgcaaaaac
    44581 ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg atataagaaa
    44641 atcggtgttg aataaatatg acagcggaac agaaaaaatc actgctactc aggcatggca
    44701 aaacgcaaaa gctttagcga aaccagctat cccttcgcag ttttctttct cgtcattagt
    44761 agatacgctt gctaatgtga agatagaaaa agcgaatttt ttagaattct ttggtggaaa
    44821 agagggatct attctagatc gatttcatgg ggaatttctt aaagataata acacattaca
    44881 tcatgaaaaa aggctaggta cggatcataa aatcaaagcg atttatacta aaaatttaac
    44941 tggtcttgac ttagagatag atgctcaaag cgttttagtt ggagtttatc cattcattag
    45001 cagttcttca gaaggagaag acgagatcac tctaccagaa gaagtcattt tcacggatta
    45061 cgtggatgat tatcctgctg gatatgtttc ttttgttgat tttaaagaca aagcgactga
    45121 tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa
    45181 accacaagtg agtggttcga ttgaattagt accattgagg catcaaagag gctatgaaaa
    45241 atttgttgat ctagaaaaag tttcgatggg tgacggagta gatgtgtatc atccacagtt
    45301 aaaagtgaat atgtcagcga gaattgtgga atatacgttt aatgttctaa ccaattcata
    45361 cgataaatta gttgtaggaa acgtcaaaac aaacttctta gaaaacacag agaataatgt
    45421 aagcaatttg attaatgatg ccattgatca attgaaaaat ggtggcgaaa tcagtgattt
    45481 aatcaatgat attgtagatc atcaaactga tatgattact ggtcaaaatg gtggttatgt
    45541 tttattagat cctaaagaag cgcctagtcg tattttgatt atggacacac cagataagaa
    45601 taccgcaagg aatgttttac aaatcaacaa tgctggtatt ggtttttcta aaactggcat
    45661 taatggaaca tatgaaaccg catggacatt agatggcgga ttcaatgcct cgtttattac
    45721 ggctggtgaa atagtaggaa ttactattag aggtactaca ttaattagtg atggcactga
    45781 ttatagaaca agtattgcta atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga
    45841 tattatggag ctagaagcac gtgattatgt aagtgctgat gccggtattg tatcatacac
    45901 catgaaaact ggtggtggtt tcatgattag aaatccacag ggtaacttgg tttttagtac
    45961 gtgggataat ggtaataaca gaccgtttct atcttttggt gcgcccaatt tcaggtatag
    46021 caatgctagt tatgtaactt ctggcgacgg tagttcttta agcattaatg gtagtgcggg
    46081 taactcatgg gaatttaagg tagctggtag gactatgaaa tttactagtg atggtatgct
    46141 aacgttgcca ggttgttttt ttggttcatg ggaagatggg aaacttgcta ggtttgaaca
    46201 atcaacggta caagtatata aagattttac tgttagaggt actaaaaact caaccgtacc
    46261 aacagaacat tatggacaac gactattaaa cgcttatgaa actccagaat attatttcgc
    46321 tgattatggg gaagccgtta caggtgatga tggtaaagtt cgtgttgata ttgaccccat
    46381 gtttgctgag acagtaaatc taagtcgata tatgacacat gtgacaccta cagaactagt
    46441 tttgtgtgct gttactcatg aagatattga ccatttcatc attgaaacta gtaagccaaa
    46501 cgtattagtt agatggaatt tagtggcaca ccgtctaggg tatgaagata ttagattaaa
    46561 agaggataca gcatatgata gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa
    46621 ggaggtatat aaatggctag cagtttatat aatttggctc tagatttcag caaagaatta
    46681 aactacacca aagctattat ggctcgtcaa ggtgataaag ggattacagt gactgttaaa
    46741 ccatttttaa atggcttgca gatggatacg agtggcggaa catttacttt aaaaggaaca
    46801 acgccatcta accgttacgt agatagtgtg gcaactagtg taactagtga agaagtcacg
    46861 ttttctcttg atggcacatt tatgagtgaa gcaggatatt ataaacactg ctacgtagaa
    46921 tatagaaaag acaatcaaat tttaacaacg caagatatca tttttttctc actaggagtg
    46981 tctgacattt cgcaaggcca agccgatgaa tacgtttcgc aattggaaga gttgattcaa
    47041 aagtataacg aaacttttga tgcttttatg gctgaaatcg aaggtagagt ggacagctta
    47101 aataaacaga ttactgattt aactggtcaa gctaaaacac tacaagacaa gttagacgct
    47161 ctgaaagaag aaatttctaa attaggtaac ttgcaggtga tgtacagtaa cagcatcga
//
LOCUS       DASJOX010000010        44934 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJOX010000010 DASJOX010000000
VERSION     DASJOX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.010"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..1189)
                     /locus_tag="U4A12_000533"
     CDS             complement(38..1189)
                     /locus_tag="U4A12_000533"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1772945.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="U4A12_000534"
     CDS             complement(1202..1639)
                     /locus_tag="U4A12_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1772946.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="U4A12_000535"
     CDS             complement(1654..3420)
                     /locus_tag="U4A12_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN1772947.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="U4A12_000536"
     CDS             3684..4361
                     /locus_tag="U4A12_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772948.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="U4A12_000537"
     CDS             4364..5491
                     /locus_tag="U4A12_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1772949.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="U4A12_000538"
     CDS             complement(5691..6131)
                     /locus_tag="U4A12_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1772950.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="U4A12_000539"
     CDS             complement(6142..8175)
                     /locus_tag="U4A12_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1772951.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="U4A12_000540"
     CDS             complement(8177..9880)
                     /locus_tag="U4A12_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1772952.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U4A12_000541"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U4A12_000541"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN1772953.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="U4A12_000542"
     CDS             complement(10963..11988)
                     /locus_tag="U4A12_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1772954.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="U4A12_000543"
     CDS             complement(12197..13066)
                     /locus_tag="U4A12_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN1772955.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="U4A12_000544"
     CDS             complement(13525..15417)
                     /locus_tag="U4A12_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772956.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="U4A12_000545"
     CDS             complement(15414..17141)
                     /locus_tag="U4A12_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1772957.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="U4A12_000546"
     CDS             complement(17157..17807)
                     /locus_tag="U4A12_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1772958.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="U4A12_000547"
     CDS             18011..18307
                     /locus_tag="U4A12_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1772959.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="U4A12_000548"
     CDS             18479..19099
                     /locus_tag="U4A12_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN1772960.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="U4A12_000549"
     CDS             complement(19191..20357)
                     /locus_tag="U4A12_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1772961.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="U4A12_000550"
     CDS             complement(20369..20722)
                     /locus_tag="U4A12_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1772962.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="U4A12_000551"
     CDS             20850..21872
                     /locus_tag="U4A12_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN1772963.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="U4A12_000552"
     CDS             21891..22052
                     /locus_tag="U4A12_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772964.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="U4A12_000553"
     CDS             complement(22311..22745)
                     /locus_tag="U4A12_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772965.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="U4A12_000554"
     CDS             complement(22855..24714)
                     /locus_tag="U4A12_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1772966.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="U4A12_000555"
     CDS             complement(24832..25992)
                     /locus_tag="U4A12_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN1772967.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U4A12_000556"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U4A12_000556"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN1772968.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="U4A12_000557"
     CDS             complement(27739..28437)
                     /locus_tag="U4A12_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1772969.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="U4A12_000558"
     CDS             complement(28394..30055)
                     /locus_tag="U4A12_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN1772970.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="U4A12_000559"
     CDS             30273..31673
                     /locus_tag="U4A12_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN1772971.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="U4A12_000560"
     CDS             complement(31925..33004)
                     /locus_tag="U4A12_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1772972.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="U4A12_000561"
     CDS             complement(33090..34049)
                     /locus_tag="U4A12_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1772973.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="U4A12_000562"
     CDS             complement(34061..35572)
                     /locus_tag="U4A12_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1772974.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="U4A12_000563"
     CDS             complement(35594..36157)
                     /locus_tag="U4A12_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1772975.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="U4A12_000564"
     CDS             complement(36163..37026)
                     /locus_tag="U4A12_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1772976.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="U4A12_000565"
     CDS             complement(37040..37936)
                     /locus_tag="U4A12_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1772977.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="U4A12_000566"
     CDS             complement(38111..39400)
                     /locus_tag="U4A12_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1772978.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="U4A12_000567"
     CDS             complement(39372..40889)
                     /locus_tag="U4A12_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1772979.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="U4A12_000568"
     CDS             41087..41977
                     /locus_tag="U4A12_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN1772980.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U4A12_000569"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U4A12_000569"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN1772981.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="U4A12_000570"
     CDS             complement(42886..44358)
                     /locus_tag="U4A12_000570"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN1772982.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14775 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttg
//
LOCUS       DASJOX010000011        44881 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJOX010000011 DASJOX010000000
VERSION     DASJOX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.011"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..465
                     /locus_tag="U4A12_000571"
     CDS             73..465
                     /locus_tag="U4A12_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1772983.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            493..1833
                     /gene="glnA"
                     /locus_tag="U4A12_000572"
     CDS             493..1833
                     /gene="glnA"
                     /locus_tag="U4A12_000572"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN1772984.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3026..4636
                     /locus_tag="U4A12_000573"
     CDS             3026..4636
                     /locus_tag="U4A12_000573"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN1772985.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            4928..5797
                     /locus_tag="U4A12_000574"
     CDS             4928..5797
                     /locus_tag="U4A12_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN1772986.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5962..7233
                     /locus_tag="U4A12_000575"
     CDS             5962..7233
                     /locus_tag="U4A12_000575"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1772987.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7230..8525
                     /gene="rho"
                     /locus_tag="U4A12_000576"
     CDS             7230..8525
                     /gene="rho"
                     /locus_tag="U4A12_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN1772988.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8624..8887
                     /locus_tag="U4A12_000577"
     CDS             8624..8887
                     /locus_tag="U4A12_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN1772989.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9332..9781
                     /locus_tag="U4A12_000578"
     CDS             9332..9781
                     /locus_tag="U4A12_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1772990.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9838..11256
                     /locus_tag="U4A12_000579"
     CDS             9838..11256
                     /locus_tag="U4A12_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1772991.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11256..12116
                     /locus_tag="U4A12_000580"
     CDS             11256..12116
                     /locus_tag="U4A12_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN1772992.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12290..13063
                     /locus_tag="U4A12_000581"
     CDS             12290..13063
                     /locus_tag="U4A12_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1772993.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13210..13575
                     /locus_tag="U4A12_000582"
     CDS             13210..13575
                     /locus_tag="U4A12_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN1772994.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13723..14931)
                     /locus_tag="U4A12_000583"
     CDS             complement(13723..14931)
                     /locus_tag="U4A12_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1772995.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14950..15228)
                     /locus_tag="U4A12_000584"
     CDS             complement(14950..15228)
                     /locus_tag="U4A12_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1772996.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15382..16077
                     /locus_tag="U4A12_000585"
     CDS             15382..16077
                     /locus_tag="U4A12_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1772997.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16519..17316)
                     /locus_tag="U4A12_000586"
     CDS             complement(16519..17316)
                     /locus_tag="U4A12_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN1772998.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17313..18497)
                     /locus_tag="U4A12_000587"
     CDS             complement(17313..18497)
                     /locus_tag="U4A12_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1772999.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18522..19769)
                     /locus_tag="U4A12_000588"
     CDS             complement(18522..19769)
                     /locus_tag="U4A12_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1773000.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            19984..21729
                     /gene="dnaX"
                     /locus_tag="U4A12_000589"
     CDS             19984..21729
                     /gene="dnaX"
                     /locus_tag="U4A12_000589"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN1773001.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21749..22063
                     /locus_tag="U4A12_000590"
     CDS             21749..22063
                     /locus_tag="U4A12_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN1773002.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22088..22684
                     /gene="recR"
                     /locus_tag="U4A12_000591"
     CDS             22088..22684
                     /gene="recR"
                     /locus_tag="U4A12_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN1773003.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22748..23392
                     /locus_tag="U4A12_000592"
     CDS             22748..23392
                     /locus_tag="U4A12_000592"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN1773004.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23407..23736
                     /locus_tag="U4A12_000593"
     CDS             23407..23736
                     /locus_tag="U4A12_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN1773005.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23750..24688
                     /gene="holB"
                     /locus_tag="U4A12_000594"
     CDS             23750..24688
                     /gene="holB"
                     /locus_tag="U4A12_000594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN1773006.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24724..25548
                     /locus_tag="U4A12_000595"
     CDS             24724..25548
                     /locus_tag="U4A12_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN1773007.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25541..25888
                     /locus_tag="U4A12_000596"
     CDS             25541..25888
                     /locus_tag="U4A12_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN1773008.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25957..26829
                     /gene="rsmI"
                     /locus_tag="U4A12_000597"
     CDS             25957..26829
                     /gene="rsmI"
                     /locus_tag="U4A12_000597"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN1773009.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26938..28059
                     /gene="dinB"
                     /locus_tag="U4A12_000598"
     CDS             26938..28059
                     /gene="dinB"
                     /locus_tag="U4A12_000598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN1773010.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28113..28715)
                     /gene="nrdG"
                     /locus_tag="U4A12_000599"
     CDS             complement(28113..28715)
                     /gene="nrdG"
                     /locus_tag="U4A12_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN1773011.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29030..31219)
                     /gene="nrdD"
                     /locus_tag="U4A12_000600"
     CDS             complement(29030..31219)
                     /gene="nrdD"
                     /locus_tag="U4A12_000600"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN1773012.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31518..32312
                     /locus_tag="U4A12_000601"
     CDS             31518..32312
                     /locus_tag="U4A12_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773013.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32302..34344
                     /locus_tag="U4A12_000602"
     CDS             32302..34344
                     /locus_tag="U4A12_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773014.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34625..34774
                     /locus_tag="U4A12_000603"
     CDS             34625..34774
                     /locus_tag="U4A12_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN1773015.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34785..36299
                     /gene="dltA"
                     /locus_tag="U4A12_000604"
     CDS             34785..36299
                     /gene="dltA"
                     /locus_tag="U4A12_000604"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN1773016.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36296..37501
                     /gene="dltB"
                     /locus_tag="U4A12_000605"
     CDS             36296..37501
                     /gene="dltB"
                     /locus_tag="U4A12_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN1773017.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37546..37779
                     /gene="dltC"
                     /locus_tag="U4A12_000606"
     CDS             37546..37779
                     /gene="dltC"
                     /locus_tag="U4A12_000606"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN1773018.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37782..39050
                     /gene="dltD"
                     /locus_tag="U4A12_000607"
     CDS             37782..39050
                     /gene="dltD"
                     /locus_tag="U4A12_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1773019.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39191..40273
                     /locus_tag="U4A12_000608"
     CDS             39191..40273
                     /locus_tag="U4A12_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN1773020.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40273..41076
                     /locus_tag="U4A12_000609"
     CDS             40273..41076
                     /locus_tag="U4A12_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1773021.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41146..41886
                     /locus_tag="U4A12_000610"
     CDS             41146..41886
                     /locus_tag="U4A12_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1773022.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41897..42901
                     /locus_tag="U4A12_000611"
     CDS             41897..42901
                     /locus_tag="U4A12_000611"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN1773023.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVGKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(42946..44790)
                     /locus_tag="U4A12_000612"
     CDS             complement(42946..44790)
                     /locus_tag="U4A12_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773024.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14427 a         7828 c         9344 g        13282 t
ORIGIN      
        1 acatatgcag ttgacaatat tttttttaat tggtacactt tagccaactt ataaaacgaa
       61 aggggacgtc tgatgggaga aaaggaattg cgccgatcta tgtccgtatt tccaattggt
      121 acagttatga agctgactga tttgtctgct cggcaaattc gctattatga agaacaagat
      181 ttagttcatc cggaacgaag cgaaggaaac cgacgtatgt attcgctgaa tgatattgat
      241 atgttgcttg agattaaaga ctatctttca gagggattga atatggctgg gatcaaacgt
      301 gtatacgaaa tggcacaggc gaaaaaacaa gaagcaaaaa acaagaagcc gttgacagat
      361 catgacgttc gacaaatttt tcgtgatgag attttggcgc aaggtgggtt aagcaaaaca
      421 ggtcattatc agtcgcaagg attgcaaaga caattctttg actgaataaa ttaaaaaagg
      481 aagagggata ccatggtaaa gaaacagata acaggtgaag aaatcaaacg aatcattgag
      541 gaagaaaacg tacgattttt acgattgatg ttcacagaca tcttaggtac gatcaaaaat
      601 gtagaagttc cagtgagcca gatcgacaaa gtattagaaa ataaaatgat gtttgacggt
      661 tcttcgattg aaggatttgt tcgaattgaa gaaagcgata tgtatttgta tccagatctc
      721 tccacttgga tgatcttccc gtgggaaagt gctcatggaa aagttgctcg tttaatttgt
      781 gatatctata atccggatgg gacaccattt gccggcgatc ctcgcgggaa tttgaaacga
      841 gcattgaaag atatgcgcga ccttggtttt acctctttca atttaggacc tgaaccagaa
      901 ttcttcttat ttaaattaga cgaagacggt gggatcacaa cgaccttgaa tgataaaggt
      961 ggttattttg attttgcacc aacagattta ggtgaaaatt gccgccgtga catcgtgttg
     1021 gaattagaaa gtctaggctt tgaagtggaa gcttcacatc atgaagtagc gccaggacag
     1081 catgaaattg actttaaata tgcagatgtg gtagatgctt gtgataatat ccaaaccttc
     1141 aagctcgttg tcaaaacgat tgcacgcaag catggacttc acgcgacttt catgccgaaa
     1201 ccgttatttg ggatcaatgg ttctggtatg cattgcaata tgtctttgtt caatgatgaa
     1261 ggaaatgttt tctatgataa agacggtgaa ttggaactaa gtgaaacagc ttatcatttc
     1321 ttaggtggtt tgctgaaaca tgctcgtgcc tacacagccg tttgcaatcc aacagtcaat
     1381 tcttataaac gtctcgttcc aggttatgaa gcgcctgttt atgtcgcatg gagtggccgt
     1441 aatcgttcgc cattagtacg tgtaccagaa tctcgcggtt tatccacacg tttggaattg
     1501 cggtcagttg atccatctgc aaacccatac ttggcaatgg cagtattgtt gcaagcagga
     1561 ctagacggta tccgtaatca gatcacaccg ccagcagctg ttgaccgtaa catttatgta
     1621 atggatgagg aagaaagaga agctgcacat atccaggact taccttcaac tatccataat
     1681 gctatcaaag aattgcgtaa ggataatgta atgattgacg cattaggaca acatatcttc
     1741 tcaaattttg ttgaagcaaa acgtctcgaa tgggcggcat tcagacaaac tgtttccgag
     1801 tgggaaagag aacaatatct agaactttat taaaacagga ataccgtgaa ttagaggctg
     1861 tgaaatcagc gttttgacct gagtattaga gaaactaacg gaaaaatagt tcctcatatt
     1921 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaggt
     1981 tgtgaaaaag ctgccctgca acaagtaata agaatagcca agcaaaaaca gcttttcttg
     2041 tttttgaatt ggctgaactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
     2101 ggttttgaaa aagacgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttct
     2161 acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaatac cgtttatcca
     2221 gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa aacagcttct
     2281 catgtttgat ggatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattt
     2341 agaaataagg aggcacgact tttgtcgcat ctccttattt tttacttatg tgattcgtta
     2401 ccttcaatga atccctacaa ggcacttttg ttgaaaaaaa ggtcatcttt gaaaaaatta
     2461 gtggaattgt tttgtttttg atattattta atagaaaaga tatgccaaaa atccgagaac
     2521 tgtggactac gttttttacg taaaggaggt aaataagggt gaaagaaaaa gattgtttct
     2581 acgataagct atataaactg ttgctgcaag taggaaaaag tagttctttg ttaggtggga
     2641 tattatctat cataatgttt gttttatttt tttatctatt atttcactta aaatttttca
     2701 gtaacgattt tctgatttgc ttaggtttaa cgttagctat gtttatatca agcttattat
     2761 gtgggttaaa ttttttttgc actcataaag gacttaagaa ataatttctc aaaggatatc
     2821 gaaaaagaaa aaattgtaag cggatagtat tcatttgagt acttgacttt cccttagtca
     2881 gcttgtatta tacaccttgg gcaccctttt agagggtcag aagtttacag aaatttcggc
     2941 tccctattct tattgaagaa tagggagctt tgtttattgt ctgagacttt tcctgcacat
     3001 aaattaaaaa ggagtttttg aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt
     3061 ttcatcaatc ggaaaaggga ttgttgcagc ttctttagga cgcttattga aaaaccgcgg
     3121 cttaaaagta actatccaaa aatttgatcc atacatcaac gtggatccgg ggacgatgag
     3181 cccatatcag cacggagaag tattcgtaac agatgatggt gctgaaacag atttggatct
     3241 gggccactac gaacgtttta ttgacatcaa tttgaacaaa tactccaacg tgacgacagg
     3301 aaaaatctat tctgaagttt tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt
     3361 tcaagttatt cctcatatta caaatgagat caaagaaaaa atcatgcgag cagctaaaat
     3421 gactgattca gatgtgatca ttacagaagt agggggaaca gtaggggata tagaatcttt
     3481 gccattcctt gaagcacttc gtcaaatgaa agcagatgtc ggtacagata atgttatgta
     3541 tatccataca acattgattc cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac
     3601 gcagcacagt gtaaaagagc tacgtggttt aggtatccaa ccgaatattt tggtggtacg
     3661 tacagagcaa cctgtttctc aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga
     3721 accagaagca gttatcgagt cgccagatgt agatacactt tactccatcc cattagtttt
     3781 acaagcacaa ggaatggatc aaattgtttg cgaccattta aaactagata caccagaagc
     3841 agacatgaca gaatggaaag aattagaaga acgtgtcttg aacttgaaga aaaaagtacg
     3901 gattgcgctt gtcggtaaat atgtcgagtt accagatgca tatatctcag tagttgaagc
     3961 attgaaacat tctggtttcg cctataattc agacatcgag atcgattgga tcaaagcaca
     4021 agaattaacg agagaaaatg tagaagaacg tttgaaagac gcagatggaa tcttggttcc
     4081 tggtggattt ggtgatcgcg gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga
     4141 aaatgacgtt ccgtttttag gtatttgtct tggtatgcag atggcttgtg tcgaatttgc
     4201 acgtaacgtc gttggattag aagatgcgtc atcagctgaa acgaatccag atacagcaaa
     4261 caatattatt gatttaatgg ctgatcagga aaacatcgag aatttgggtg gaactttacg
     4321 ccttggatta tacccatgta aaatcaaaaa aggaacgaag acggctgaag catatgatgg
     4381 agccgatgtt gtgcaagaac gtcaccgtca ccgctatgag ttcaataaca aatatcgtca
     4441 attgtttgaa gaacaagggc tagtcttttc aggtgtctca ccagataacc gtttagtaga
     4501 aatcgtagag ctgccagaga aaaaattctt tgtgggatgc caattccacc cagaattgat
     4561 ttctcgtcca aatcgcccgc aaaaattaat caaagcattt gtcggagcat cattagcaga
     4621 tcacgaaaca aaataatttt tgaacttgat agaaagagaa aagtagcaag agaaggggat
     4681 aagcaatttt cttgtcactt ttctcttttt ctgatttgtt attatttgta ggaaaaaaga
     4741 tagaaagcaa aagacttttt tcgattaaag cgctattgga tattttattt tttgcaaaaa
     4801 acttcttgaa aattttcatt ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa
     4861 aatagggtcg ttggttaaga attgtcttaa caaatttagt tttacaaaac ttaggaggaa
     4921 ttttattatg ccagtagtat caggagctga atttttaaaa gctgctcgta aaggcggtta
     4981 cgctgtcggc ggattcaaca caaacaactt ggaatggact caagcaatcc ttgaagcggc
     5041 tgaagcaaaa caagcaccag tacttatcca aacatcaatg ggtgcagcta aatacatggg
     5101 tggttacaaa atctgtaaag atttaatcac tgatttagtt gaagcaatga acatcactgt
     5161 tccagttgct atccacttag accatggtga ttacgaagct gctttggaat gtatcgaatt
     5221 aggctatact tcagtaatgt tcgatggttc acatctacca tttgaagaaa acttgaaatt
     5281 agctaaagac gttgttgaaa aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg
     5341 ttcaatcggc ggagaagaag acggaatcat cggtaacggc gaattagctg atattgaaga
     5401 atgcaaacaa atggttgcta caggtatcga ctacttagct tgcggtatcg gtaacatcca
     5461 tggtcaatat ccagaaaact ggaaaggatt agcttttgac cacttacaag caattgctga
     5521 tgctgtaggt ccagacatgc ctttagtatt acatggcgga tcaggtattc ctcaagatca
     5581 aatcgaaaaa gctatctcaa tgggtatctc aaaagtaaat gttaacactg aattccaatt
     5641 atcatttgct gcagctactc gtaaatatat cgaagctggt aaagataaag aaggtaaagg
     5701 attcgaccct cgtaaattat tagctccagg taaagcagca atcgttgaag atgctaaatc
     5761 tcatatcgac tggttcggtt cagctaacaa agcgtaagca tccacataac agatacgata
     5821 tataggaatg gagaacgact tttgaagtcg ctctccattc ttttttactt aaaacggcag
     5881 accatgctcg tttctttttt ttatctatga taaaatacaa gcatatccat gaaaaaagat
     5941 taataaacaa ggtggaaaca gatgaaaaaa atcgtaatca aaggaaaccg acctttagct
     6001 ggtgaagtaa cgatcagcgg tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc
     6061 ttagctgatt ccccagtgat cttggaaggt gtgccggaca tacaagatgt tcattcatta
     6121 attgaaattt tagagatcat gggcgcaact gtccattttt ccaacaacat actagaaatc
     6181 gatcctcgag aagtcgtatc gattccgatg ccgcacggaa aaatcaatag tttgcgtgct
     6241 tcttattatt ttatgggaac actgcttggt aaatttggcg aagcggtagt aggcctgcca
     6301 ggtggctgtt atttaggccc tagaccgatt gacctacacg tcaaaggatt tgaagctcta
     6361 ggtgctgaag tgacaaatga acatggtgcc atgtatttac ggacagaaaa taaaggattg
     6421 cgtggcaatc ggattttcat ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta
     6481 gctgctgtaa aagcaaaagg acagacagta attgaaaacg ctgcacgaga accggaaatc
     6541 atagatgtgg caactttact gaataatatg ggggcaaaag ttcgaggagc gggtacagat
     6601 gtgatccgta tcgaaggagt cgaaacatta catggctgtc ggcatttcat gattcctgac
     6661 cgcatcgaag caggaaccta tctagcatta gcagctgcag taggaaatgg tatcaaagtg
     6721 aaaaatgtga tctttgaaca tttggaaagc tttatagcaa aattgcagga aattggtgtc
     6781 catatgaaaa tcagcgaaga tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca
     6841 aacatcatga cctatcctta tccaggattt gccacagatc tgcagcagcc acttactgcg
     6901 ttactattga tgactacagg cacttcagaa atcatcgata cgatctatgc gaaacgtgtg
     6961 aaccatgtgc cggaactagc acgaatgggc gctgatatta ccgtggaagg aaatatgatc
     7021 atcgtgaatg gtccaaataa acttcacggc acagaagtcg tggcttctga tcttcgggca
     7081 ggtgcttgtt tagtgattgc tggactattg gcagaaggga caacgacgat ttacaatgta
     7141 gattatatct tacgcggtta tgatcatatt atcgagaaac taacagcctt aggggcatca
     7201 attgaaatga tagaggaagt gggagctgaa tgagcgatta tttgacaatg gcagaactag
     7261 agaacaaaac gttaaaagaa gtttatagtt atgccaaaga atttaaaatt ccttattaca
     7321 gtaagatgaa taaaaaggaa ttatctctag ctgtgattcg tgcacaagca gagaaacaag
     7381 gattttatta tatggaagga atattggata tcgttggtca agatggttac ggttttttac
     7441 ggccaatcaa ttatggacca agtgcagaag atatctatat ttctgcctca caaatcagac
     7501 gattcagttt gagaaatggg gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg
     7561 aacgttatta tggtttgatg cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa
     7621 aagaacgtcc gcattttcca gccttaacac ctctttatcc gcaaagacag cttcaactgg
     7681 aaacaacacc gcaaagacta tctacccgaa tgatcgatat cttttctcca atcggttttg
     7741 gtcagcgtgg attgatcgtc gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa
     7801 tcgcaaatgg tatcaccgaa aactatccgg aagtagaatt aatcgttcta ctgattgatg
     7861 aacgaccgga agaagtaacg gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta
     7921 catttgatct gcagccgcaa aaccatactc gagtttctga attagtattg gaacgcgcaa
     7981 tgcgtttagt cgaagataaa cgagatgtcg ttatcttgat ggatagtatt actagacttg
     8041 ctcgggccta taacttagtc gttccaccaa gcggacgtac attgagtggc gggattgacc
     8101 cagccgcatt gtataaacct aagaaatttt tcggggcagc aagaaacatc gaagaaggtg
     8161 gcagtttgac gattttagcg accgctttag tcgatacagg aagccgtatg gatgacgtga
     8221 tctatgaaga attcaaagga actggaaatc tagaattaca actttcacgt gaattatctg
     8281 aacgtcggat cttcccagcc atcgatatca aaaaatcagg tacccgaaaa gaagaattat
     8341 tgatgccaag tgaagaatta gaagaaattt ggaaacttcg aaaaaatatg actggagatt
     8401 cattagaata tacggaacaa ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat
     8461 ttttcaaaga atttcaagat gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa
     8521 gataattgca accttgactg aaacatgtta agatgttaat gttgtaaatc gacaaatcaa
     8581 ctctggttca gcaaatggag cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca
     8641 tccagattac cacccagtag tattcatgga ttcaacaact ggtttcaaat tcttgtcagg
     8701 ttcaacaaaa acttcacaag aaacagttga atgggaagac ggtaatacat acccagtcat
     8761 ccgtgtcgaa gtaacttctg actcacatcc attctacact ggacgtcaaa aattcacaca
     8821 agcagacgga cgtgtggacc gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc
     8881 ggaataataa aaacgctgat aactcaacgt ttagcagact atctgatagg gatagtctgt
     8941 ttttttatgt tttttgccaa tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat
     9001 aatgcacaaa acaatgaaaa actagtatat agttttaatt atccacttga taaaaacacc
     9061 ttatattaac gaaatgaaga ggggttattt cgaagtaaaa aaccatttac ctaaaattag
     9121 aagctaagct ttcctttcaa ggaaggcttt ttgttttgtt atagacttgc gattgtttgt
     9181 gagctagatc gttcatttta atgacttata cacgcggatg atgggtcttt ttcctctgtt
     9241 cattcataat attgaacggt acagctagcg atgctatact gacctcagtt ccaagaaaac
     9301 gttaacattt tttgtggggg agaggttttt tatgaggttt gatcaattga tcgcagaaga
     9361 actgattgtt ttcgatccat cgatagattc aaaaggatca ttatttgagc gaatagggga
     9421 tgtgttgaaa aaaacaaata aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa
     9481 acgagaggag gagatctcca caggaataga agatggattt gggattcccc atgcaaaaag
     9541 caaatatgtg acaaaaccag cactgtgttt ttttcataca gccaaattaa cggactatag
     9601 cgggatagat gaaatgccaa ttgaatatat ttttgccatc gtagttcctg aaaaatcagc
     9661 agatatgcat ctagagattc tcagcacact ctccagacga ttgatgaatg cagattttag
     9721 aaagggaata aaagaagcca ctacgccagc agatatcatc agacagatta gtcaagaatg
     9781 aaaatagtag agtctttgtc cgagtatcga tagtgaagaa aaaggaggaa aataatcatg
     9841 aaaatcgttg gtgtaactgc ttgcccgact ggaattgctc atacttatat gtcggcagaa
     9901 aaattagaag taacagcaag aaatctaaac catcaagcaa aattcgaaac acaaggtgta
     9961 aaaacagaaa atcagttatc tgaacaagaa atcaaagagg cagatgcaat cattcttgca
    10021 gtagataaag aaatcgaatt ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca
    10081 agccgagcaa tcaaagaacc gcaagtagta atagacgaag cattgagaga tattggcgta
    10141 ttcgttgtgt ctaatgaaaa agaaccagct gcaaatgaga aaaagccgac aatctataat
    10201 catttcatga atggtgtgaa ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc
    10261 gcactgagtt ttgcctttgg gattacagcg gctgatccca actcagcaga ctataatgtg
    10321 ttagcagctg cattttcaag gatcggagga gatacagcgt ttgccatgat ggttcctgcg
    10381 ttaggcgcgg ggattgctac atcgattgct ggaaaagcag gttttgcccc aggaattgtt
    10441 gccggattac tggcctctac aggtggttca ggttttcttg gcggaatgat tggtggcgtg
    10501 ctagcagggt atatctgtga ttttctggca aataaaatta gcgtaaaaaa agaattttct
    10561 gctatttatc agctgattgt cgtccccttt ttatcgatct taattattgg gcttttgatg
    10621 gtatttgtga ttgaacaacc gatcacatgg gtgttggatg ctttgacaaa ttggctgaat
    10681 agcttgggaa atacatccgg gctattgttc ggattactta tcgggatcat gatggcagct
    10741 gatatgggtg ggccgatcaa taaatcgact tcgactttct cgatcggttt gatgtcagct
    10801 ggagtaaatg caccgattgc tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt
    10861 gctttagcca ctttattatt caaacataaa tttaccaaag aagaacggac ttcaggtcaa
    10921 tcatgctgga ttttaggcgc ttcgtatatt acagaaggtg ctataccgtt tgcggtatct
    10981 gacccgatcc gcgtgatacc tagtttgatg acaggttcag cagtcgctgc agggatttcc
    11041 atggcagtag gagttacttc aatggcacct catggaggga tttgggtgat gctaattcca
    11101 aatgttatga acaatttacc tatgtatgtc ttggcaattg catcaggtac agtcactaca
    11161 gcctttatgc ttggactatt gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg
    11221 aaaaatgaaa atctaacaaa tgaggtggaa gcgtaatgtt atatacaatg aaagagttat
    11281 tgaaagtggc taaagagaat caatttgcag taccagcctt caatatttgc agctatgata
    11341 tgatgaaagc aatcatcgaa gaagcagaga gattagaagc accagtaatc ttagaaattc
    11401 atcctgatga aatcgcctat ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt
    11461 atacaagtaa actgccgatc gttatccact tggatcatgg cggtacgata gaagatgtct
    11521 tacgggcgat tcgaaacggc tattcttccg tgatgatcga tgcgtctttg aaatcattcg
    11581 acgacaatgt acagattacg aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag
    11641 tggaggctga gcttggaacg attgggaata atggttctgc tgaaggaggt gctgaaacaa
    11701 tcatttatac agatcctgat caagcgaaga tttttgtgaa agaaacagga atcgatacac
    11761 ttgctgtagc aattggaaca gcgcatggct tgtatccgaa aaacagaact ccaaaattaa
    11821 atatcgactt actgaaagaa ttgaaccaac aaatcgatct tccatttgtt ttacatggcg
    11881 gttcaggtaa tccggacgca gaagtcagcg aagcagtaaa atacggagtt gccaaagtga
    11941 atttgagctc agatcttaaa agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa
    12001 atccatcgat gtacgagccg aatgcaattt atccagcagc taataaaaag gtacaagaag
    12061 tggtcaaaca taaaatggaa attttgaaca cagtaggcaa ggctagttta tactaaaaat
    12121 aaaaatgtgt ggtcaagagg gtatgacaat agtcttgtca taccctctta accacacacg
    12181 atgttcagaa gtagtttttt cgaaaaattt cttcttattt ctcgaagcca atcacttctg
    12241 tcacaacctc ttttttcctg ttactatata aagtaaatgg ggtgatttta tgttgaatga
    12301 agtggagaat agacagcagg agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt
    12361 caccgatttg atcgagttag taaattatag cgaagccacg attaaacgtg accttgtttt
    12421 tctggaaaaa agtggattga tccgtagagt tcgcggtggc gccatgctta ttgacaatcg
    12481 aaaaattgac gtgccttacc tgatgaagat caccaaatta gaagaagaaa cagaaaaacg
    12541 taagattgca gatacagctt cttctttgat tcgagatgat atggtcttat tcttggattc
    12601 cagtacaacc tctttacact tagtcagaaa cctagcaaga tttgatggtt tgcagatcat
    12661 tacgaatggc atcgtcacag ctgctatgtt gagtgaatat acaacggcaa aagtatctgt
    12721 attgggaggg gcaattgttc cgaaacgagc aacaatcaac ggttcaaaag cctataacga
    12781 tgcattgtca tattatgcgg atttagcgat aatgagttgt cgcgggctag atttcagcca
    12841 aggagtaaca gaaactcatg aaggagaggc aatggtcaaa aaagctttta gaaaacaatc
    12901 agaccggctt gccgttttag ttacacagca aaaaattgca cataaattca tgcatcaagc
    12961 cattgctttt catgaaatcg attatctggt gacaagtaaa gaactgacaa aagaacagta
    13021 tgcgaaagta aaggaacacc atatcgaatg tctgtacgga taaattatta cgtgaatgtt
    13081 ctaagtggct tcctcatttt tagataggac cttatctttg ctcgcttctc tcagccgatg
    13141 gtataataat gaaaaggtta tcttgttaga taatcttact tttttatcca ggagaaagga
    13201 gtgcagaaaa tgaaacatgt gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt
    13261 ctgtgggtcg tgctgacagg tttatacggt gttagcttta tggattcaac gattgttggg
    13321 gtaattattg tagtcatgat ctatgtgcta ggggatctgt tgatcttacg taaagtaggg
    13381 aacatcgcag cgacgatcgc agatgctggt tcagctgcag ttatcttgtg gctctatcta
    13441 tattttatga atgatacagt cgatatttgg atgaaagtat tgattcctgc tctattgatc
    13501 ggtgtggcag aatggttctt ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa
    13561 cggaaaatgg aataaatcaa cagttaaact attcttatcg ataaatgaaa gctttaaaaa
    13621 taaatagatg tttaattaag aataatcata aactattttt actcaaaaaa ccgctcagat
    13681 tattttcaga tctgaacggt tttttttatc tattcatcaa gatcaaattt catttttcaa
    13741 tgattcaatc aattcgccct ctgcttcatg actatgtttt cggccattaa caagatagag
    13801 tgctgtacat atcaatggaa tgactccggc aatcacatac aatcctctaa atgaagtaag
    13861 agattttagc atccccatca aataaggacc aacgcctaag cctaggtcta aaccaataaa
    13921 gtaggtagat aaagcaatac tgattcgatg ttcgcggacg atttttaaac aaacagcctg
    13981 tccatttgac ataaacgtac cataacctag gccgactaat gctccagaga taagcaatgt
    14041 cacactattc gtcgttacac tcagtaaaaa taatcctgct gttaagaata aatagctagg
    14101 atacatcacg tattgttctc ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa
    14161 tgtgatgacc aaagcatata cgacgaagaa aaatgaccca gcagcaacta agtggattac
    14221 ttttgtataa gaagataaaa atgataaaac acttgaataa gaaagaccca tcaaaaaggc
    14281 aattcctgta atgaaaagtg ctttcttttc aataaaacta ttgatattcc atgagcgaag
    14341 agatattttt tgttcttcag ataatggaat attttttaca ggaaagacaa ggcaggcaat
    14401 cgtaacaaag aagaccagta caattgaaaa ccagataatg aaacgaaaat cagtcatatt
    14461 cagcaaaatc attcctaaaa atggcccaac agcggcagct aaactagtac ttaatccata
    14521 ataattgatg ccttcccctt ttttagatgc agggatatat gcagtaacga tagcatttgt
    14581 agctgtagaa actgtaccgt agccgaatcc attcaagaat cgaactaaat acaatgcccc
    14641 gatagttggc gtataaagat atgctaatgt tgaaaggaga tagaagatcg cgccgcctct
    14701 taaggtaaac ttccgaccaa atagttctaa ctgtttcccc atgaataagc gtgccagcaa
    14761 tgttccgatg atatagatcc cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt
    14821 gtcttgcgca atgactgcca tgatgaccat caacagataa taaatcaggt agacaataaa
    14881 attgatcaat gtgattccaa taaaatattt attaaataat ttcttttcca tgtatgttat
    14941 acattcccct taagctgaat atgcttttaa tttttgtatt tgaacttcta catcgtgata
    15001 attggatgga tagcgatcaa caagttggcg aataaataaa tctcttgtag acataaacag
    15061 atttaaaaat tcttcgcatt cgtttgttgg attctcatcc actgctttga tcaattcgat
    15121 cgagttttga tacgcatcat ttaaaaagac taaagaagct ttcatatcaa atcttgtttg
    15181 atatgctgtt tctaatcgat taatgattgt gttcatccag taattcatgt tttttccctc
    15241 tattcttaaa aaattttact tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat
    15301 aagtgatgga aaaaggtact taacaaatgt taatatcatt agtaaaatac ctttggactt
    15361 taaaaagttt taaagtactg tatgatcttt ttagatgaaa gtttggatgg gggagcatca
    15421 ttggatagta gagtcttaaa ggaatatttg gataaaaatg attttccgat tatacgcaaa
    15481 ggatatcata aatatttgac attcgaaggg atggaagatt cccatacgta tatcttgaaa
    15541 gagggaacgg taaaagcaag tgttatttca aatgatggga gagaatttaa ccttcgctat
    15601 atcaaagatc ttgaaatcgt ctctctttta aaagacgaat attcacaatt catcgattcg
    15661 ccgtataata ttcgagttga atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc
    15721 ttttggcaaa atatcaacca agacccaaag ctacaacact atgtaaaaga gtattaccgc
    15781 tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga
    15841 gctgtttgta cgcaacttta cgaattatat gatttattcg gtgtgcggac agaagaaggg
    15901 atgctgatcg atttttcgat cacgaatgaa gaaatcgcca aattttgtgg catctcaact
    15961 gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag atggtattat caccgttaaa
    16021 gaccatcata tcgttatttt gaatatggac ttgttagaag acaatattat cttttgatga
    16081 acaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggaaaaata
    16141 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    16201 taccaagagg ttgtgagcct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
    16261 agcttttcat gtttgatgaa tattgtgact tgtgaccgag gagttggtag gagaacaccg
    16321 tttaccaaga ggttgtgaaa aagctgttct gcatcaagta ataagaagag ccaagtaaaa
    16381 acagtctctt atgtttttaa cttggctgga cttatcaccg tagatgtaag cttttgaacg
    16441 ccgtttattt gcaacaaaag gggctgtgac acgatttttg tcacagccct ttttttgcga
    16501 acattcattt agcaattttt agaaacttct gccgccaccg ccaaaagtcc cgccaccgct
    16561 agaatgggta gtacttccgc caccgccgct tccaggaggt gggcttttgg ggattcttct
    16621 tgtcgtgaca aaagaatttg tcagacgatc ggttttttca gttaatttga tactggattt
    16681 ttctcggaat ggatatttgt agatgcctga tttcaattga taacgcgaaa cagtcacaaa
    16741 ataaaagaca agactaagga taagagccaa aacaacagca atgatgattt ctactgtcgt
    16801 caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct acacgataat gtcctcctgg
    16861 aactccgtct tccacatatg ttgctgattt agacaggtaa gcttttgcag cattgtaata
    16921 ggcagcattt gtcatctgat cataaacatc atctaagatc gaatcgattc tgctatcagt
    16981 caaataatcg atcatattac cagaagtcga aatgtagatt tcacgctgtc ccatatctaa
    17041 tagcaatact gagccatttt gatcatttcc gactgcgtct cttaagtaat tatcagcaaa
    17101 gtctcttggt tcttctgaat tagatgtagt cgttacgatc aagactcgtc ctttgatttt
    17161 ttcattcagc ttttctgcct gttgttccaa agattggatt tgatctgttg tgaataaccc
    17221 agcctcatca tttaccgtag ctgtatctgc atgagtagcc accggccagc caacacttac
    17281 tagcaaaaga aagaataaaa aaaacttttt catcatatca agtaacctcc gatcagaaag
    17341 aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc ccaatttttt ctggctgatc
    17401 ggcaggacac cgctgacctt tcctgtttgt ccattcattg cgtagtagta gactttttta
    17461 cttgagtcat tgcttcgata agtaacaagc cataccggca gtaatacgta attatttact
    17521 tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg ttgtatatcc atttgcggta
    17581 tcttttaaca gcttctctga atagtctttt aattcagatt gaatctgtga tttgattgcc
    17641 tcatattcga tatctctttt ttctgcttgg aacccggcta aatactggct tttaaaatca
    17701 attgctttat ctaacgggaa aggttgcacg ccttcgacca tcttttgttg cgtatttttt
    17761 gataaggcat tttttactag ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat
    17821 tgcttcgttt ctgtgtattc gatgtcacct atccgccata tgcggataac tgtaccattc
    17881 gcctgcatgg caccatctac ttcagcatct gtagcccaat aagggaaata cacacctgtc
    17941 aacttatcga tttgatcctt attgaaaaaa gctttgggaa caaaccattt tttcttggtc
    18001 caagcgagaa atctgttgat tgcttcttct ttctcgacag caaaaggaag tactttattt
    18061 ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg gattatggca gtagtaacaa
    18121 taagtcgcag cagttgtcgc atcagtcacg atctctgcac cacagttggg acaaagaaaa
    18181 agatctatcg ctgcggtatt atctgcagat tcgtccgctg tatctgaaaa accgcctgcc
    18241 tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa
    18301 gtaaaatctt cggtaggtga aggttcttct ttgctttctt cgccagccat acgcgcttct
    18361 ttttgctttt gctcatattg gctgacttct tcttctgtat agatattcag gcagtattcg
    18421 caatgaaatt tttggtcttt gggatcaaaa gtcaacggac cgccgcaatt aggacattta
    18481 tgtgtaagaa catccatcag ctgcacacct ttctaaataa attaattaac ggggattcct
    18541 ttaaacggtt taccacagtt tggacagaat ttagggatgc cgttggaaag atccacgatc
    18601 tcattgcatt cactgcatcg catttgtagc ttaggctgaa cgttttctac aggtttggca
    18661 gctccgcagt tggaacagaa ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa
    18721 gtatccgcat ttccttgtgc agtttgttga ttttgttttt ctgcttgttg tttgatttgt
    18781 tcttggttat tttgagctgc ttgagaaaga taagaaccat ttgcattcat tcccatacct
    18841 acacccataa agccggtcat tgctcctgct tcattttttc cggctgcttc cataccacgt
    18901 gcaatggaac cttgcacgta tccttcacgt acgctaggat cacctagcat cgccccttcg
    18961 ttacgcatat tgatcagttt cacagaatca tcggtgtaag aaatgctggc tacagccaca
    19021 gaaacaatct ccatcccacg taattcacgc caagaatcat ccaatgcagt atccatgtat
    19081 ttgcttaatt ccaaactctt agatggtaca taagaaatcc gttgaccatc tgcagacatc
    19141 tgattgattg ctgattgtag cgctgttagg aattcagcta aatactgctc attgatatca
    19201 ttgatttcca cttgtgtttt gtttttaggg attgcgtttg tataaaataa aatcgggtca
    19261 acgacatgaa tagaataagt gccatgtgcc cgtaagaata attctgcgtt atagaaattg
    19321 tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc cctttatttc ttggagattg
    19381 atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt
    19441 tcagcaattg cttctttcag tgttccattg aacatggaag gagctgcgtc atttttaatg
    19501 gtgtaataac cttcctcagc ggtataatcg atgatttttc caccatcgac caaaagcatc
    19561 atcatgtttg gatagacatg gacgactgtt ccgtctgtta aaacatcttc tgttccttta
    19621 cgattggagc cacgtttgtc atctttgcgg actttgacac cttttgtcat gaccgttgta
    19681 tcactcatat tgtctggttc gatgacttct agccattgat ctgccagacc gccgccaacc
    19741 atacttgttg ctgctttgat aagacccata aaaaattcct cctctgattc agctagtgcc
    19801 aaacaattat tgtttattta ttataccata ggaagatgtg aaacttctcg gactagagac
    19861 ggaattgaaa tggaaaattg cgtattgttt aaatacctga aaaagccgag aaggatagta
    19921 gtttttttac ctaatatcac gtataataag agatagtgaa ttttttaaaa aagggggatc
    19981 cttatggcat atcaagcact ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt
    20041 ggacaaaaag ctgtgacaca gacactaaaa aatgctattg tttcccacaa aacctcacat
    20101 gcctacttgt ttacaggacc cagaggaacc ggaaagacga gtgcagcaaa gattttcgca
    20161 aaagcaatca actgtccgaa cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt
    20221 cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac
    20281 ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg
    20341 tacaaagtgt acattattga tgaggtacat atgctttcaa caggagcttt caatgccttg
    20401 ttgaaaacat tggaagagcc aaaagaaagt gtcatattca tcttagcaac gaccgaacct
    20461 cacaaaatac cagctacgat catttcacgg actcagcgat ttgattttaa acggatcaat
    20521 acggcagata ttgtcgaaca tcttgaattt atcttgaaag aatcgactat cagctatgaa
    20581 ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg ggatgcggga tgcattgagt
    20641 attcttgatc aggcaatctc gtttagtgaa ggaacaatta ctttagatga tgccatgcaa
    20701 gtgacaggta gcttaactga cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa
    20761 gaagtaccag aagcattgga aacattgaac caaattctcg cagccggcaa agaatcacgt
    20821 cgctttctag aggatatgtt acaatactgc cgtgacttgc tgatgtatca gcaagcacct
    20881 aacctagttg aggaacaatc gagtacgatc agaagtacat ttacagaatt ggcacaaact
    20941 gtttcagcgg atcagctgtt ttcaatgata aagattttga gcgatactca aaatgaaatt
    21001 cgttttacca atagtgccgc tatttatttg gaagtcgcta cagtcaaact tgcacagcta
    21061 ccgtctgctt ctgttccttc agcagatgta gcatttgcag atgatcagat agtaaaacag
    21121 ctacaacaag agattaagga actaagaaac gaaatcaagg aactacgcag taatacagga
    21181 attgagaata caaaagtaca gccggtacag caaacaaatc ataaaaaaca gacaagtacc
    21241 tatcgtgtac cgacggaacg agtatataaa gttctgcggg aagcgacaag acagcatctg
    21301 aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc tatctgtcac tcagcgagca
    21361 atgcttaaag caagcgaacc agttgcagct ggacctacag gactagtgat tgcgtttgat
    21421 tatgagattg tttgtcaaag agcagcaaat gacgaagaat tgcagctttc ggtacacaac
    21481 aacttaagcc gaatgatcaa agattatgcg cctgatcctg tttatattac aagagaaagc
    21541 tggccagaac ttcgaagaaa ttatttgacg caggcacaag aagagggaac agaaaactct
    21601 tttgcttctg aagactcaga aggagaaatc gagttgcttc ctcaagaaca agaagtacca
    21661 gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct ccgaagctgt tacagtggta
    21721 gatgattaat aaaaaggagt agataattat gatgcgtgga atgggcaata tgcaaggcat
    21781 gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa
    21841 aacagagttt acaggtaaag ccaccaatga attggtcaaa gcggttttta caggcgatcg
    21901 tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat cctgatgata tcgagatgct
    21961 tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca caaattgatg tggaaactga
    22021 gaaaaaatta gggaaatacg caaaaggttt gccaggattt tagttttaaa ctgaaggagt
    22081 aatgaatatg caatatccag aaccaattgc gaaactgatc gacagctaca tgaaacttcc
    22141 aggaatcggt cagaaaacag cgacaagact cgccttttat acgatcgata tgaaagatga
    22201 agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag
    22261 tatctgcgga aacatcacag aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga
    22321 tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg
    22381 agaatatcac ggactttatc atgttctcca tggtgtgtta tcaccgatgg aagggacagg
    22441 tccagaagac atcaatattt cttctctgtt gcagcggctg catgatgcag atgtaaaaga
    22501 agtgattatc gcaacaaatg caacaacaga gggagaagca acagctatgt atctctcacg
    22561 tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca catggtctgt ctgtaggctc
    22621 tgatatcgag tatgcagatg aagttacttt attgaaagct gtcgaaggaa gacgtgagtt
    22681 ataaatttct aattaagtgt tttttcagat acaatataaa agcagcacaa gagatggggg
    22741 caacaatgtg aacggactat ttattacaat cgaggggcct gatggtgcag gaaaaaccag
    22801 catcttgaat gaactttacc ctcttttgaa agaagtagca aaaacagaaa tcattcaaac
    22861 aagagaacca ggaggcattc cgattgctga agagatccga gcagtgatcc ttgatccaaa
    22921 aaatgatcgg atggatgaac gtacggaagc actgctttat gcagcagcaa gaagacaaca
    22981 tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa atcgttttat gcgatagatt
    23041 tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc atcggcgtta ccgagattgc
    23101 tcggttgaat gagtttgcta cagaaggtac tacgccggat tttacccttt atcttgacgt
    23161 agattcagac accggattaa gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt
    23221 agattcggaa ggtttggaat tccaccagcg tgtacgtcat gcttacttaa agctggcaga
    23281 agagaatccg gaaagaatcc acaaagttga tgcaagaaaa agctttgaag aagtgctgca
    23341 aacaagttat catacaatta ttgaacagta tccgcaattt tttgaaaact aggaaggtga
    23401 acgtttatga aaattatttt agcgattatc caagacaaag acagcaaccg tttatcaaat
    23461 gaattgattg atgcaaatat ccgtgccaca aaactatcat cgacaggcgg cttccttaag
    23521 gctggaaaca gtacgtttat cgtgggtatt gaagatgacc gtgtagaaga agcattggaa
    23581 atcatcaaaa aaacgtgtga atcaagaaag caatttgtat ctacacctgt tacactggat
    23641 atttcgatgg atgggggcgt cccttatcca gtagaagtag aagtaggcgg tgccacagtc
    23701 ttcgtcttac ctgttgaagg attccatcaa ttttaggagg agctgagcta tgaatcaaga
    23761 aataaacttg gctcaaatgc aaccgatcgt gtatcagcag cttcaacgga gttttgagca
    23821 tgggcgtatc gctcatgctt atctttttga aggggagaaa ggaacaggaa aacatgaagt
    23881 aggtatatgg cttgcacagc acctgttctg tacccaaatg aaagacaatc tgccttgtgg
    23941 aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat ccagatgttt tggttattga
    24001 accggaagga caaaccatca aagtcgatca gatcagacgt ttgcagacgg aattcagtcg
    24061 cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag
    24121 cagtgcggca aacagtttgt tgaaattctt ggaagagccc cctggagatt ttttagcggt
    24181 gttagaaaca gatgcagtag gaagaattct ccctactatt caatctcgtt gtcaagttct
    24241 gcattttcaa gaactttcga aagaagcact gatccatcga ttgcaagaag agcagattcc
    24301 attggaaaaa gcaaaacttt tggctttctt aacaaacagt ttaggaaaag cagttgaaat
    24361 ctctcaagat gaatggttta atgatgctaa ggacttgatt tatcaatggt tcgtttactt
    24421 gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa aaattggtaa aatcatttaa
    24481 agaaaaaaca caacagttta caggattatc tgtcttgatg ttttattatc aggaagcaca
    24541 gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa cagataaacc agataattga
    24601 acggatattg atcgcagagc agaaactacg cagcaatgtc agttttcagg ctgttgcgga
    24661 acagttcgtt ttgcatacga ttttttagct tgccgcaaag gatgatgatc aaggaggatg
    24721 gaaatggtag aagtagtagg tgtccgatat agagatgcag gtcatatcta ttattttgca
    24781 ccaggagact ctgaatatgt ttatagtgac aaggtattag tcgaatctca gcaatcctta
    24841 catattgcta ctgttgtcct tcctaaaaaa gagattgatc ccaatgacct tcctgaagct
    24901 atccgtccga tcttgcataa agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa
    24961 aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa
    25021 atgaaaatgg tcaaagccga atacacattt gatcgaaata aattgatttt ttctttcaca
    25081 gcggacgggc gaattgactt tagagaattg gtaaaagatc tggccgctgt atttcgtact
    25141 cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa agataatagg cggaattggt
    25201 ccatgtgggc gtccattatg ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc
    25261 aaaatggcaa aagatcaaag tttatctttg aatccaacaa aaatttccgg tttatgcgga
    25321 cgcttgatgt gctgtctgca atatgagagc gaagaatacg agacagcgaa aaaagaactc
    25381 cctgactttg gaaaagtaat tgctacacct gatggtaaag ggaaagtcgt agggttgaat
    25441 ctgcttagcc gtatcgttag tattcggctg actggacggg aaaatcctgt cgaatatgaa
    25501 tgggatgaat tgaaagaatt tatccaagta ggtgagatga atggataaga gatcattata
    25561 cgacggattg aatcaattag aatcagagtt gagaaataca ttaacagaac ttgttgaaat
    25621 gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg
    25681 tctgcgggaa cacctgcggg aattgaacca agttgcacaa gctccagctg aaaatgtgaa
    25741 gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt tatgaagaag gattccatgt
    25801 ctgctacgat ttatatggat caagaaggga caatgacgag ccttgtgcgt tttgtctaga
    25861 agtgatctat cgcgaaagcg gtcgatgacc aataacaaga ggagactgga gcggagaaat
    25921 actgctccag tctttttatt aaggagattg tgacagatgt ataatcaaaa aagtttcaag
    25981 ggaaatccgg tatatgggga tttatatctt gtaccgacgc caattggaaa tttagaagat
    26041 atgagctaca gaagtgttcg tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg
    26101 cgtaatacac aaaagttatt gaatcatttt gaaatccata caccccaaaa aagtttacat
    26161 gaacataatt ataaagaacg tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg
    26221 attgctcaag tcagtgacgc aggaatgcct tccatcagcg atcctggaca tgagctagtt
    26281 ttagcctgca taaaagaagg gatttctgtg atctctatcc caggacctac agctgggatg
    26341 acggctttga ttgcttcagg gctagtgcct caaccatttt tattttatgg cttccttcca
    26401 cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa aagaacagac agttacgctg
    26461 attttttatg agtcacctta tcggatcact gctacattga cgaatttttt agaaattttt
    26521 ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga
    26581 ggaagtacgg aagagctact cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt
    26641 ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg aagttgatga agagttggga
    26701 acgttaaagg aacaagtaga agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc
    26761 aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa
    26821 ttagactgag atagtataag ttgtctataa tgaaagacta atgaggaaat cagctactaa
    26881 cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata atgaataaga ggtgaggatg
    26941 atggagctgc aatttccttt acataatgat acatcaagaa aaatcattca tattgatatg
    27001 gatgcttttt tcgcttcggt tgaagaacga gatcatcctg agtttcgcgg acatccatta
    27061 gtaattgcca gacaccccag tgacactggt gggcgtggag tagtgactac agcaaattat
    27121 gaagcaagaa aattcggaat tcattcagca atgagtgcac aaaaagcgta tgagctttgt
    27181 ccgcaagcca tctttaagcc tggcgaccat cataaataca gcgatatttc caaacaagta
    27241 cgggaagtat tctatcgtta tacagatatt gtggaaccag ttagtatcga tgaagcgtat
    27301 cttgatgtga ccgaaaataa aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc
    27361 caagcagata tttgggagag tgtccagcta acatgttctg cgggagtaag ttacaataag
    27421 tttttggcca aattagcatc agactatcaa aaacctagag gaatcacggt agttgcccct
    27481 caagacgctg tgccattttt aaaagctttg ccaattgaaa aatttcatgg ggtagggaag
    27541 aaaactgtac ctagaatgca tgagctaggg atttatacag gaaaagacct atatgaatgc
    27601 acagaaatga tgttgattcg aaattttgga aaaatgggct attctcttta tcgtaaagtc
    27661 agaggaatcc atgattctcc agtaaacgtt acaagagagc gaaaatctgt gggaaaagag
    27721 catacttatg ggcagccgtt gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta
    27781 gctgaaaaag tagaagaagc actgagaaga gtccaaaaac atgggaaaac agtagttttg
    27841 aaggtcaggt acacggacta ttctactgtg accaaaagag tgacattgcc agaatatatc
    27901 tataaaaaag aagcactctt ttatcaagca agccttatat gggaggaaat cttgggagta
    27961 gaaaaaggaa tccgattgtt agggattact ttgacgaatc tggacccgat gacttatgaa
    28021 aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa aacagctttg agagttgaaa
    28081 ccggaaatcc gttttctctc ccaaagctgt ttttatatca agtcttgctt tttgatttgt
    28141 tcataggttt ctgttgcatc cgtacatttt tcccaaatca caacttgatt ggattctaaa
    28201 gatttttgga cgtcaatgat ccgctggttg ctacttccgc gaaattgtag ttttaggtca
    28261 cgtttgctta attcaaagcg tccgtcaaca aggatatcga tttgcgaaag gagttctaat
    28321 ttatcttctg aatcttgtag caattcatca aaggcatagc ctgtccatga ccagatatct
    28381 tttgtcgtac cgaactcttt tcggatacgt cgtacgactt gcagacaaac atctgtattc
    28441 aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt aatcgttgcg caagtcagcg
    28501 atgatttgtt cttctaattc aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct
    28561 ttgttgaagc agccttcgca ggcaaataag cagccactaa cgtaaagact gttgcggaca
    28621 ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa tatagttttg gctgtagtct
    28681 tttgctaacc attcttttgg ttttggattt ctcataaaaa aacttctttc atttaaggtt
    28741 caaagatata gctattgaag ataaaatcta ctcgaccaca taaataagaa caaactgacc
    28801 taaatccatt gttcaagaaa gcttgttctt attttgatag agggcgttca aagatacaaa
    28861 gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc tcttattctg aataaacggt
    28921 gttcaaaagc ttgcatctgc agtaataagc ccaactaaac gaaaaatatg aggaactatt
    28981 tttgtttagc tattcttatt acttgatgca ggacagcttt ttcacaacct catttcatgt
    29041 gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga gcttgcggat
    29101 ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca tgatttccac
    29161 attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa ccacattgat
    29221 agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc
    29281 atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag tagtggatga
    29341 atccacctga acaatatttt ggataatcct tctcaaattc caatttttca aatggcgttg
    29401 ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct gtgatatttt
    29461 cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga ctttcacttg
    29521 gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct gcattagctt
    29581 ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc
    29641 caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat acagtagctc
    29701 gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc ccgaaagaac
    29761 catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt tctacacgat
    29821 agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag aatttattct
    29881 gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca cctaaattca
    29941 ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag aatgaacgac
    30001 agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa ttcagaatat
    30061 caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg taattaggat
    30121 cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg aagatagctg
    30181 tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt tgaatttctc
    30241 tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg
    30301 aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag atatcttttt
    30361 tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat tcttccgcgt
    30421 cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca tctgttcggt
    30481 cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt gagatttgtg
    30541 ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt ttgaacccat
    30601 tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta taaggatgat
    30661 aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata tgtggtggca
    30721 acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc tgtgtattga
    30781 aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg ttgattagct
    30841 tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt ttggtacgat
    30901 aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata tgttccacga
    30961 tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt tcttggacaa
    31021 ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag ccgtgaattt
    31081 tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca acatggcggc
    31141 catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg acagtttttt
    31201 cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc tatcataaaa
    31261 caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg tgctgtataa
    31321 ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga aaacttgtag
    31381 ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga ataaaatttt
    31441 ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta gatatgctac
    31501 tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa aagatgatcg
    31561 aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa gccttagata
    31621 atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca agtggtgcag
    31681 gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg ggcagtgtct
    31741 cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa
    31801 aaaaactagg attcattttt caagagttca atttgatcga taccctaaat gcaaaagaca
    31861 atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa aagagagtaa
    31921 gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc gatgaacttt
    31981 ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag ccacaagtga
    32041 tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa ttgttgaact
    32101 acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc catgatccat
    32161 atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt ttttctgaag
    32221 ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg caggcgacga
    32281 ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt ttttcggcag
    32341 tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat ctattattcc
    32401 ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga tacttcgata
    32461 gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt tttcgtattc
    32521 agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta tcagcttttt
    32581 ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat aattggattg
    32641 ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt ttcaatgatt
    32701 ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat tccatcagta
    32761 gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata ttcaatatgg
    32821 aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc agatagttca
    32881 acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact acttttaacg
    32941 agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat cagttatcat
    33001 tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg tgtggtcggt
    33061 acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag taaatggaag
    33121 atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca tttgctcaaa
    33181 agttggcgta caaattctct gattacagtc attctcggaa tttctttaat gacaattggc
    33241 ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc cccgatggac
    33301 tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga ggaagggcag
    33361 aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta tagtcaaaat
    33421 ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata tcaagccttc
    33481 agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca tactgttttg
    33541 cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag tattggactg
    33601 ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg tgacgaaaca
    33661 atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa agcaaaaggt
    33721 gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa
    33781 cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc ttatgatatc
    33841 gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga ccaaatgaag
    33901 gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta ttcgaaattg
    33961 cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt cgggatgatc
    34021 gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc agaggaagaa
    34081 aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat cagacggatg
    34141 atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat cactcacgca
    34201 gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg gttggcttat
    34261 cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac gagtcagtta
    34321 tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa tagaactaaa
    34381 ttttgaatac catttatttg gaataagagg ttgtgacacg atttttgtca caacctcttt
    34441 attttttata ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg
    34501 ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc tgtaagctag
    34561 acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat gaggagggaa
    34621 tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat tcatcattcg
    34681 aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact ataaaaatat
    34741 tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa atcaaaacga
    34801 ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct tatcaggcgg
    34861 atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct tattatttag
    34921 aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag tttgaaatga
    34981 ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt gaagagcata
    35041 cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg gtcatcagta
    35101 tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag
    35161 aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt acagaaaatt
    35221 tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa atcagtcatg
    35281 ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag cctggtatgc
    35341 atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt tatcctgcat
    35401 tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac tttcgtcaat
    35461 tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca tcatttatgg
    35521 atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg gacatgtttt
    35581 tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa
    35641 atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt tcaagtattc
    35701 agattgacca gcatgtacta gatagttatg accgactacc gattggctac gtaaaaaaag
    35761 acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa acgggtgaaa
    35821 tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa aagacagctg
    35881 ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca ggtagactag
    35941 atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa ttacatggat
    36001 accggattga actcggggat atcgaacatt atctgctcca agacaaccga atcaaacaag
    36061 ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct tttgtcgtat
    36121 tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa gaacaattac
    36181 tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat ctgctgcctc
    36241 agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat
    36301 gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag ccattgcgct
    36361 tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt atcaatcagc
    36421 agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac agggagcggc
    36481 gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg tttatcggca
    36541 aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa ttccactgtt
    36601 tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg gttttttagg
    36661 aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag atggcatgat
    36721 caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa caatctcttc
    36781 tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc ctgttcgtga
    36841 taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa
    36901 attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac gcattgcctt
    36961 acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca gtatgtattt
    37021 gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca tgatgggata
    37081 tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag aattctggaa
    37141 tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc gattgatgtt
    37201 cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata tcggttattt
    37261 tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca tcgtatatgg
    37321 actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta agaagaagca
    37381 taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt tgacatttca
    37441 agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt tttttagcta
    37501 acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga agaacaaatt
    37561 ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat
    37621 ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt cgagctagac
    37681 ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg gagcacaccg
    37741 aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag aaactttttg
    37801 ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt acctcctcct
    37861 atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga
    37921 acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa tatattcctt
    37981 tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt ctggcagaaa
    38041 aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa tcgttaagcc
    38101 aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta
    38161 tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt
    38221 ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct
    38281 atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga
    38341 ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca
    38401 gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc
    38461 gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat
    38521 tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga
    38581 aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa cagtcacaaa
    38641 aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat
    38701 tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg
    38761 attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat
    38821 taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt
    38881 ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca
    38941 gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt
    39001 ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg
    39061 cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc
    39121 aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg
    39181 gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg
    39241 agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc
    39301 tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg
    39361 ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac
    39421 cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc
    39481 tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa
    39541 accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt
    39601 cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga
    39661 tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa
    39721 agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa
    39781 agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg
    39841 aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg
    39901 tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg
    39961 cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt
    40021 tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg
    40081 aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt
    40141 tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac
    40201 agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg
    40261 gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt
    40321 tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg
    40381 agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag
    40441 cacttattga gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt
    40501 tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt
    40561 ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac
    40621 tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat
    40681 atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt
    40741 gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt
    40801 attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg
    40861 gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt
    40921 ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt
    40981 cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga acaagcacgg
    41041 attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa
    41101 aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt
    41161 ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa
    41221 tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa
    41281 caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt
    41341 gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca
    41401 ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa
    41461 aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg
    41521 attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa
    41581 acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat
    41641 tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat
    41701 catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag
    41761 aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg
    41821 caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac
    41881 atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa gaatcaattt
    41941 agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta
    42001 tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg
    42061 agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt
    42121 ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt
    42181 ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc
    42241 tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa
    42301 cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga
    42361 tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat
    42421 caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa
    42481 aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt
    42541 agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc
    42601 tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc
    42661 tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat
    42721 gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtggg
    42781 aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat
    42841 tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg
    42901 aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata caggattcgg
    42961 gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt cctgcgttag
    43021 tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac gttcctgatc
    43081 atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg cactatgttc
    43141 gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt ttcgatacag
    43201 tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg
    43261 agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga aggttatctg
    43321 aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg tcacctggtg
    43381 gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct tttccacata
    43441 gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca tctcttggac
    43501 aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct cataatgttg
    43561 ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt tgtcggtatt
    43621 taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa tatataaaat
    43681 cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt aaatcaagca
    43741 atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac ggttattctt
    43801 atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac tagcaatatt
    43861 agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc
    43921 gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat agtaaaacat
    43981 tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata aatgagataa
    44041 aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg atacagcttc
    44101 aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa gacgtttttg
    44161 tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa cagcgattat
    44221 catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg gtccagttgt
    44281 tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg agactgtaag
    44341 ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg agagtccggg
    44401 attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta gatatacagt
    44461 aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt tttcaagtgt
    44521 cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag tgtacttgct
    44581 gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt ttatttcttt
    44641 catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa
    44701 aaataacaat gataatataa tgattatgct ccactttata cgtaaataaa aagattcttt
    44761 tttattatat atgttattaa aaaaagacat aaaggtattc ctttctaaaa cttgtaatgt
    44821 tagggtaaac gttttttaaa ctaacgtcaa ttatttataa taaaaaatga gataaagatg
    44881 a
//
LOCUS       DASJOX010000012        41260 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJOX010000012 DASJOX010000000
VERSION     DASJOX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.012"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(192..662)
                     /locus_tag="U4A12_000613"
     CDS             complement(192..662)
                     /locus_tag="U4A12_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN1773025.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="U4A12_000614"
     CDS             992..2632
                     /locus_tag="U4A12_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN1773026.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="U4A12_000615"
     CDS             2786..4108
                     /locus_tag="U4A12_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN1773027.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="U4A12_000616"
     CDS             complement(4176..4610)
                     /locus_tag="U4A12_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773028.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5512)
                     /locus_tag="U4A12_000617"
                     /pseudo
     CDS             complement(4675..5512)
                     /locus_tag="U4A12_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(6254..6880)
                     /locus_tag="U4A12_000618"
     CDS             complement(6254..6880)
                     /locus_tag="U4A12_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773029.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7175..7510)
                     /locus_tag="U4A12_000619"
     CDS             complement(7175..7510)
                     /locus_tag="U4A12_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1773030.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7497..7781)
                     /locus_tag="U4A12_000620"
     CDS             complement(7497..7781)
                     /locus_tag="U4A12_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1773031.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7837..9360)
                     /locus_tag="U4A12_000621"
     CDS             complement(7837..9360)
                     /locus_tag="U4A12_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1773032.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9353..10528)
                     /locus_tag="U4A12_000622"
     CDS             complement(9353..10528)
                     /locus_tag="U4A12_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1773033.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10532..10717)
                     /locus_tag="U4A12_000623"
     CDS             complement(10532..10717)
                     /locus_tag="U4A12_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773034.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10683..12377)
                     /locus_tag="U4A12_000624"
     CDS             complement(10683..12377)
                     /locus_tag="U4A12_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1773035.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12374..12847)
                     /locus_tag="U4A12_000625"
     CDS             complement(12374..12847)
                     /locus_tag="U4A12_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN1773036.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12916..13071)
                     /locus_tag="U4A12_000626"
     CDS             complement(12916..13071)
                     /locus_tag="U4A12_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEN1773037.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13205..13585)
                     /locus_tag="U4A12_000627"
     CDS             complement(13205..13585)
                     /locus_tag="U4A12_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1773038.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13589..13804)
                     /locus_tag="U4A12_000628"
     CDS             complement(13589..13804)
                     /locus_tag="U4A12_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773039.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13807..14214)
                     /locus_tag="U4A12_000629"
     CDS             complement(13807..14214)
                     /locus_tag="U4A12_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEN1773040.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14480..15955)
                     /locus_tag="U4A12_000630"
     CDS             complement(14480..15955)
                     /locus_tag="U4A12_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN1773041.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15945..16793)
                     /locus_tag="U4A12_000631"
     CDS             complement(15945..16793)
                     /locus_tag="U4A12_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEN1773042.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16830..17192)
                     /locus_tag="U4A12_000632"
     CDS             complement(16830..17192)
                     /locus_tag="U4A12_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773043.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17193..17351)
                     /locus_tag="U4A12_000633"
     CDS             complement(17193..17351)
                     /locus_tag="U4A12_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773044.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17492..18116)
                     /locus_tag="U4A12_000634"
                     /pseudo
     CDS             complement(17492..18116)
                     /locus_tag="U4A12_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18113..18424)
                     /locus_tag="U4A12_000635"
     CDS             complement(18113..18424)
                     /locus_tag="U4A12_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773045.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18468..18761)
                     /locus_tag="U4A12_000636"
     CDS             complement(18468..18761)
                     /locus_tag="U4A12_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773046.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18955..19602
                     /locus_tag="U4A12_000637"
     CDS             18955..19602
                     /locus_tag="U4A12_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1773047.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19663..20808
                     /locus_tag="U4A12_000638"
     CDS             19663..20808
                     /locus_tag="U4A12_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1773048.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20889..21194)
                     /locus_tag="U4A12_000639"
     CDS             complement(20889..21194)
                     /locus_tag="U4A12_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN1773049.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="U4A12_000640"
     CDS             complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="U4A12_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN1773050.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21647..21913)
                     /locus_tag="U4A12_000641"
     CDS             complement(21647..21913)
                     /locus_tag="U4A12_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN1773051.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22168..22893)
                     /locus_tag="U4A12_000642"
     CDS             complement(22168..22893)
                     /locus_tag="U4A12_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1773052.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23254..23324)
                     /locus_tag="U4A12_000643"
     tRNA            complement(23254..23324)
                     /locus_tag="U4A12_000643"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23290..23292),aa:Gly,seq:ccc)
     gene            23441..24193
                     /locus_tag="U4A12_000644"
     CDS             23441..24193
                     /locus_tag="U4A12_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1773053.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24209..24688
                     /locus_tag="U4A12_000645"
     CDS             24209..24688
                     /locus_tag="U4A12_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1773054.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24939..26519)
                     /locus_tag="U4A12_000646"
     CDS             complement(24939..26519)
                     /locus_tag="U4A12_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1773055.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26512..27255)
                     /locus_tag="U4A12_000647"
     CDS             complement(26512..27255)
                     /locus_tag="U4A12_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773056.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27431..28207)
                     /locus_tag="U4A12_000648"
     CDS             complement(27431..28207)
                     /locus_tag="U4A12_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773057.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28455..29291)
                     /locus_tag="U4A12_000649"
     CDS             complement(28455..29291)
                     /locus_tag="U4A12_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN1773058.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29302..30231)
                     /locus_tag="U4A12_000650"
     CDS             complement(29302..30231)
                     /locus_tag="U4A12_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1773059.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30423..30776
                     /locus_tag="U4A12_000651"
     CDS             30423..30776
                     /locus_tag="U4A12_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1773060.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30850..31506)
                     /locus_tag="U4A12_000652"
     CDS             complement(30850..31506)
                     /locus_tag="U4A12_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1773061.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31732..33666
                     /locus_tag="U4A12_000653"
     CDS             31732..33666
                     /locus_tag="U4A12_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1773062.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33752..34975)
                     /locus_tag="U4A12_000654"
     CDS             complement(33752..34975)
                     /locus_tag="U4A12_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1773063.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34969..36867)
                     /locus_tag="U4A12_000655"
     CDS             complement(34969..36867)
                     /locus_tag="U4A12_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN1773064.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(36861..37979)
                     /locus_tag="U4A12_000656"
     CDS             complement(36861..37979)
                     /locus_tag="U4A12_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1773065.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(38062..38895)
                     /locus_tag="U4A12_000657"
     CDS             complement(38062..38895)
                     /locus_tag="U4A12_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773066.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38888..39805)
                     /locus_tag="U4A12_000658"
     CDS             complement(38888..39805)
                     /locus_tag="U4A12_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773067.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(39828..40007)
                     /locus_tag="U4A12_000659"
     CDS             complement(39828..40007)
                     /locus_tag="U4A12_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773068.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(40032..41156)
                     /locus_tag="U4A12_000660"
     CDS             complement(40032..41156)
                     /locus_tag="U4A12_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1773069.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12380 a         8304 c         6866 g        13710 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     5101 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5161 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5221 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5281 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5341 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5401 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5461 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5521 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5581 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5641 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5701 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5761 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
     5881 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
     5941 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
     6001 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
     6061 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa acccatatca
     6121 aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag ttatctatca
     6181 ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa acttcaataa
     6241 gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga gctacataat
     6301 ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt tcccaagata
     6361 acgtaaatag atttgactct ggacttataa agtctacttc accttctagt attactttct
     6421 tatgcaaata ttcctctaat aaatatagac ttgctaaagc atacaacaca ttttccaaat
     6481 ttgcttcctt ataatttgaa gaacgaccat gttttactct gttatatatt ttccaccaat
     6541 ctggtgtatc ttcaggccac ccatctccaa aaggttttag tgaagtcaat gttaaagaat
     6601 tcataaccct aacaccatta tccaaacaca ttaattcgct agatttaata acagctttaa
     6661 aatcagttat gcttttattg ctactgctat caaatccgca aagttctttg aaaataatct
     6721 cacactccga accaatcgct agtagtagtt tttgatattc aatagagaat gttttgaaat
     6781 tattctcatc gagcatcaca aaatctgtgg tattaataaa ctcattttct aaatgtagat
     6841 aatacttcca ataaaaattt gtaaactctg ctttgtccat taaatcacct cttttgaatt
     6901 ataacaaaaa aaacacgtcc acaacagacg tgttctttga tttctacaca atcagcttgc
     6961 caaaaccatc caatcagtcc tagcatgcct gtattaaaat tgaacgttca tcatctatca
     7021 tctttcttag tccagcatgt ttgcaaccac acctacaatt ttcagtccaa caatagattt
     7081 cgactagcta agtcttgcaa aattcaggca gctaaccctt catttactac cttaattata
     7141 ctaaataccc acaacgttta caagtgtaat cattctaaga aatctgttta gctatgatcg
     7201 ttgtataatc tttaaacgcc gttccttcat tgattgaaat aattttataa ctcttgtttt
     7261 tccaagctac tttcatatcg tttgttagtt cataatcttg atcgtatcga ataataaacg
     7321 tcaacgttcc ttcaagaatg gtacctacac tagcaagcac gtcttttagc atttgtgaac
     7381 gcacattagc ccaacattca aatacagttt cttcactatc caccatctgc ccatcttcat
     7441 cttttacccg ttttgtcttt ttaaacttga ctctctcagt caattcattt acattcttaa
     7501 tgattggcat aataagcacc tctcatttgt tgtactaatg cttgtaccgt tactggtatc
     7561 cgttcgctgc tggcttcttg acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc
     7621 aaatcaaatt gctgataatt atccatatct ttatccgttg cgctactatc aatagcactt
     7681 tttatatatt cgcctgctgt atcaatcaat tgttgaataa agtcatcatc aatagtgtga
     7741 tcgatccgca tacttttttt tatgctatcc aaaattacca ttttatctct cctaatgaaa
     7801 aaaaggcagt gggataaacc gccgcccaat atttttctat acttttggtg ttacaactgg
     7861 cgtaaactca atatacactg aagcattttg atcaattttc ttataatcgt tgcgaacgat
     7921 cactgcaagt ccttgtgagt agtaatcgaa tttttcccat tgagtcgtta cttgatttct
     7981 acgagcaaca aagatagatt gtgcaatatc ccccataatc atagggaatg ttccagcttt
     8041 aggattggca aacaatgtat ctgcaatcaa tactactggc ataccgaata atgatttacc
     8101 actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc
     8161 taggtggtaa tatccgcttt ggtttagaat taccatttta tttaatgctg ggtctaatgc
     8221 cacattgtat agttgtttca aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt
     8281 taataaatcc atgatatgct tattgtcagt gttttctacc aatttcgcta actgatcttt
     8341 gacctcttgt acaatattca ctgctgaatc ttccacaacc tcatttgata aggcaatctt
     8401 accagcacga gtttctactt tataatcaac tgaagtaaac atttcagcgt caatatcacc
     8461 aatttcagca agttcagctt ttgttgctaa cactgcttgt tggttagttg ctactggata
     8521 tttaccttgt ccatttgata ctgtttttac tgttgcatat tgagctaagt tataatttga
     8581 acgtttcaaa tcaaagactt caccgattac ttcttcggga acaactgccg ctgcatttac
     8641 agtagtgact ccatcacgca cttcgccttg tgaacggata tattcttcat acccacgcaa
     8701 ttctgttttt tcgttatcga ttaaagtttt ttccatgtta ttactctcct tttgtgcttt
     8761 agaagtggat tctaagctat tgtttgtttg ttttgcttgg ttgttactca taaacgattc
     8821 gtaagaacgc ttatttactt ggacatttga tgaatcataa gcgggaacgg tcacgacact
     8881 aatttcatta agtgctttga ttttatctat tgatcgttca attgtgccat cttctttttt
     8941 gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata gaatcaacaa ccccttttga
     9001 gatattctca tacacatcat ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa
     9061 tcctgtatca tcgacattca atttcaacgt acctgctttg acactagcta gtggctttga
     9121 ataatcgtgt ccatataata agaacacatt tgataaatcc acctctttta atgcttcggg
     9181 tgtaatcact tctacaaagc cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata
     9241 gcctgaaata gttttgccgt ctgttggttg tttttcattt tctttctcta ctttgggaga
     9301 atcggctgtt agctcagctt cttctgtcag ccgtttttcc ttttcttctt cattattcaa
     9361 ctggtgtaac tccttcctgt tcttttaatt gatatttctc caacgtatct aaatacgtaa
     9421 agttcaaact tgctagtaaa cgatcgccac catccatggg gggtagcccc attttcgacc
     9481 gcccctcatt aatcgtgaga agtgaccctt tgacctgatc caatacattt tttacttttg
     9541 tttcaggatc agtctctagt aactgatcgg aattaaagcg gatattggtt tcaagtttcg
     9601 tatctaattc actcacaaac acattaaagt aatgaatcag cgtactttgg atatactgga
     9661 gattactttg aacggtgcta gaatgttcat tttcaacgcc taaacgctca atcggtacac
     9721 caaacgcctt ggcaatttgt tttgtgttcc aatcattgga attgataagc ttcaaaacat
     9781 cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc aagaatgatc gttctaagag
     9841 cattatctcc actacttgaa ccgttggctt cttcaaactt ttcacgaatt gcgttctttg
     9901 ctgatccgtc taaatctgac ttatgaacct tcaaaatacc tgacccactg acaccacgag
     9961 taaagaaatt gtgcaatgtg cggttgccag cttgttgaat tttcagttca tcacgtaacg
    10021 cataaagtgg tggtaatcct gttaaaccat cttgggtgaa atatttgaag tgcaaaatat
    10081 cgctagactt cacacgtctt tttttatcac caatctgata agacaacgta ccattatcta
    10141 attgagtaac cgttacactt gagttaggta ataagtgaat ttcttctacc ttttcaccgt
    10201 tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat attgaccgcc aaagcaaact
    10261 ttaaatgcca accatccatt tctgaattag gtttctcgtt tagtaacttc accagttcat
    10321 tatcagcttg tggcatattg tttttgacca attgaatcgg acttgaagca atatcactag
    10381 caataattcg aacggctgca aacacatcac tatttcttaa tgcacgaacg cttgtaaagc
    10441 tggtggtgta atcatccgat tggattgata ccacatgttg taggaaagga tctcccgttg
    10501 tattttgtct cgctttaaaa aatgccatct tttaacctcc cttctgttct gtatttagca
    10561 tgaccgctac cccaataagc actatgccca gcgctaacaa gccatagtag acatttgtta
    10621 gtaaggtgat agcaaccaac acacacacca atccaagaag taaaagaacg gtatgaatgt
    10681 aattagaaag aaaattcttc acttgtataa aattcattgt ctgctttcgc tccttccgtt
    10741 gtggtgaagt aatccattgc aaacacatag gcattgatta atgcagctat tggatcaatt
    10801 ttgttactgt tctttgattt attaatctga atgccgttgt tatccgtttt taaaatagcg
    10861 ttgttcactg catgggtgag aatcgtattt ttattgtgaa taacgttgcc ttcataaact
    10921 tgttctctaa aagttcgggt cggaacgttt agagtaatcg ttccttgtct tacttctaac
    10981 attgggtagt tggctttttc agctttactg ataagcgaat tagcgttata cgggtcataa
    11041 caaatcccca tacattccaa atcattttct tgaatcaaat cttgaataaa ctgaaatact
    11101 tggtcatagt ccacgattcc actttctaat tgagtaatgg aacactcacc agctttttct
    11161 agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt gaagcccata tttggtggct
    11221 acaaagctat gtgaatcaca ataaagttcg ccgttatcta gcggtacgat ccatgaaacg
    11281 ctagttaaat catctgtttt agataaatcc actccgatat aaacgggctt gcctgtaata
    11341 tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa tcatatagct gtcctcactc
    11401 gcttgtctcc ataaattgaa gtttttcacc aatacagaat taaggttatt ttgtttcaaa
    11461 gcaaggttca catcatcttg aatggttgga atcattactt ttttgatttc ttcactttca
    11521 aaaatcggat tggctttgat ccatttctct tgatcgtgaa tttcttcggg atcgtccaat
    11581 tcccatatcg ctataaagta cctgtcggct tgttcttcgc cttttagcac acgatctaac
    11641 aataagtatt cctcgtacat aggaacgttt aaatcaagcc cagcggtact aataacacac
    11701 aataagccgt tcttttgttg ggtcattcct gacttaatga cgttgtacgt tttacgagtt
    11761 tttgcttcgt gccactcgtc aagaattgcg gttgttggtg caaacccatc tagcgtgctg
    11821 gtttcactag ccagtgccat agcgaacgaa ttagagggca agtgctgaat cttagagttc
    11881 ataattttca gttgtggtct taaaaactta ctagacttga ccacgtttct aagtgaatta
    11941 gacagcatat catagcccaa tttagcttgt ttcaaagcgt tacttacaaa caatacttgc
    12001 cttgcttctg ctggttcttg ttccataatc aacgcattgg cagccatgcc acttgcgata
    12061 tacgttttcc cattcttacg gctcatactg gtaaacgctc gattgaatcg cctgtaattc
    12121 ccttctttag tacgccagcc ataaagactg cctagaatga atttttgaaa tcctaacatc
    12181 ttgattgctt taccgtccgt agatggtagc aactcaatga atttaatcgc tttggtggct
    12241 tgttcttcat cgaacacata agggaagcta tctgacttag aacgctccaa atcgcttata
    12301 tgacgctcac acgcttggat aattttcttg ggtgcaatca gcttacctga taggactttc
    12361 tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt
    12421 taaccttgtt caaagctagt tttgatcgtg attctaaggt catacctagt gaggtggctt
    12481 gttttttcaa atcactcata gcttgtgatt gaacccgaac cgctggattg gctttcttta
    12541 ctccgttttc gcatgtgata aacgtacctg attttcttat ctcggcttcg gctgttttca
    12601 ttcgagcaaa ggctaaacaa taggctacca atgaaccata atctgcttca ctcaatggat
    12661 aatctttttt taatgttggt acgatacgat tccattcact tctagcgctc gctgccatcc
    12721 agtcaggggg cgtgatattt attaatggtt gctgcttgaa caattcttct ctagcgtcca
    12781 cacggattgc tttttcttca cttgatatat tgccttttgt atcgtgtaat attttagggt
    12841 gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac gtaagcaatt aagctcacat
    12901 tccttcgttt ttcgatcaga atttttcttt atctaattta tctatataaa tgatttttcc
    12961 gttctgcatt tgaatggtca cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc
    13021 tgttttatca gtaataacaa ttgtattttt caataaatct cctactttca ttatatactt
    13081 tttcacttgt gattacaagt gtaatcgtta tttgtaatta cccttatgcc ttttcacaac
    13141 ggttatttgt accaaaatga gtaggcatcg attgtcagta agtttcacac tatgccccct
    13201 taactcaaag ggtactcttt tctcgtcttt gtctttcatt ggctgtcttg gaattatggt
    13261 gtgcatggca taaagaacga aaattagttt catctaaccg tctattccaa tcatctttta
    13321 gttcaatttt gtgatctaca acatcagctt tccttatcaa gccttcttct aggcaatcct
    13381 cacatactgg atgatttaac cgatacaagt atgaagcctt tcgccatgtt ctactttgat
    13441 agaattgaaa atatttacct tcgctttgct ttctttcttc acgcttaatt gccgtttgct
    13501 ttgctttatg cttcccacaa tacttctgcc tataatcaat caatgtttta catccagcat
    13561 gattacattg ttttttaact gccatagatt accaaccaaa gcgatctcgt tcgtcagaat
    13621 catcttgttc ccattttgat ttgtattcaa tataaggcga aactatcttc aagcacgttc
    13681 cgtctttaaa attaacttta gtgattttat cttcctcaaa actaataaag tctacatctt
    13741 cttgagtgtt tgaatactca atgtgtttct caccatcgaa gtatctaata ctttccatat
    13801 gcataattat tctacctcca ataaattagt tatcaattca tcatctgtta gtagcaaagc
    13861 caagaactta tcatggatgt ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg
    13921 tctcgctcga tcatataggt taagcagaca attaaattca tccattagct cacctgtatc
    13981 atcatagttg ccactatcta ccaatgatcc cactactagc gattgacacc acgctactga
    14041 ctcctgcagc caatcaggtt cttcttccat tatctttaat acttctggat caaatgtgga
    14101 ataatcttct gttaccaatc ccacactaaa tgccagcgtg ttcattagaa accgatccat
    14161 ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa ataatattga gcatttactt
    14221 tcctccttga ttcgtaacac gtggtaacac ctacgtaaca ggtacccgtt accactcaat
    14281 cccaacgggg acaagggttc tagcgttttg gtaacaggta acgggtaaaa tccagtaaca
    14341 ttattcccca gcatattagc taactatttc aaaagtttta aggaaataag gtgttacccc
    14401 gttaccaaat tgctgtatcc cttggggcag taagggtaaa ctagtaacgg gctaggtgtt
    14461 actgacccgt tactacccgt tactttttgt actttccagc tcgattttta ttttcatagg
    14521 catttgactt attgtaaaat cctcgtcgtt tatttctttt tggcagttgc ttccgttccc
    14581 aaccaggaat actgctaata atcaaggcaa tctttttagt ttctgcatct gatcgaccat
    14641 tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc
    14701 tatcaactaa ataaaactct tgttgcatag tagctccatt atagaaatgc tgtaaatttt
    14761 tctcattaca tttactgtca ggatagttct gataataaga acgtctcgcc cacgctggag
    14821 cttcttccca tttatacggg atttccattt ccaaatattt agtgattgct tctttaattg
    14881 gattctccat cttggcatcc tcttgatagt cttttgcaat ttctaagact tctttatctt
    14941 catcgggact aaaatagatt cgttgtcctt tatcaaaaag cacctttgct tcagctaaca
    15001 cttgaagaaa atattcatcc tttgttttaa acggattcat ctttggctta ttcttacaag
    15061 gcaaaggata aaaacggcgg tttcctgtat catctttcaa atactgccca tcattcgacg
    15121 ttccaataaa tacacattgc cttgcccaag ctatgacatt ccgaccataa ggaggtctaa
    15181 ttttatcgat cttggcactg atgaagtttt ttatctgttc aattttagtt ttgttcattg
    15241 aagataattc tccaagttca ataattacat tgccttgaag ctgcatataa tcatctttct
    15301 tttcacctag cgaatctaat gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa
    15361 cggtactctt accaatccct tgcggtccat ccaaaactgg aacgatctca aactttattc
    15421 ctggtttata gatacgtgcg actgatccga ctatccaacg ttttgttact tctcgtgtgt
    15481 aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat
    15541 cccatttaac tttttcgata cgttctttta ctgggttgaa aacattcttt ctagccacat
    15601 ttactactgc tttgtgtatt ttgttgtcat caaaaactac attgtagttt tcttcaatat
    15661 atgccgttaa ggcatcctca aaactgtctt cccattctcc agcggtacga ttgatccaag
    15721 gagaatcttt taataggtga atgtatccgc taaattcgtt ataagcaatt gttccagcta
    15781 aattgctatc attggttaaa atcaattctg cattcacaac actgttactt ttaatctgat
    15841 cgtctttatt gcatcttaat cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac
    15901 gctcgctggc ttcgggaaag tctaaaatac tatctaattt atgcttatga gccatgagct
    15961 ttccccctca aatctttttt aatcatcgat cgaataattt gattcacttc tttatctttt
    16021 agcggtggtt cattgtacgc atttgctgta taaatcaatt ctgctaaagt ttgattatct
    16081 acaccgtgcc atagtaacca accgcataac ttagttaaat gatcgtttct actaccttcg
    16141 ccagcgcctt gaacaatcat atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg
    16201 tattgagtct ttatttcttg cggttttttt agcagtaggt tcactagcca atcgggagct
    16261 ggtgcaatat tatttagaga tacgttttca ataggttcat atgctctccc aacacttctt
    16321 gaaataatca tagttggcgc tattggaact cctttagtga taatatcaat cccgcttaaa
    16381 cttttacctt tatcatcacg cccgaagata tcagcaaatg cacctgtttc ttgttggata
    16441 attacaccat caggaatttt aaagaagaaa tggattcctt tacctggtgt gatttccgta
    16501 taagtagaag gaaatggatc atacattgaa gcaagttttt tataattctc aacgccgttt
    16561 cgtgtttcag aatgttgatc taggtcaact accaccattt gatgatcttc aagcattaac
    16621 ccaatatttc tagttgcatc ttccgtccac cattcattaa tttgattaga atcgttagtt
    16681 gcatcattcc aaccactaga gccttttatt ggaattttag ttccaggaga taagggataa
    16741 attgaccatc ccgacttttg taattccgtt gctgcttgat aattatttgc caataaacct
    16801 ctcctccttt ttcaaaatac tggtataaat cattcagaca cctccatgag cttcaactgc
    16861 tcaagttcgt atttcaaccc atcccgaaca aattcagcac cttttaattg attctgatac
    16921 tttacccatg tattaaatgt tttcaccctt gcttcttgca tatcagtaat gtttaaccat
    16981 gcggttaaaa tctgattttt tagctcgttg atttctcgac aaattatttt gtgttctgca
    17041 tgttttttga gtacatacaa aactgttagt ttttcattga atgaatttcc aaagtaggtt
    17101 ttcccgatat tattttctag ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta
    17161 tcgagttggt ttaatagttt tgttttgtac atctacttat gccctccagt tcttaattga
    17221 tagtcgtact cgtcataagc agtgaagaga tataacgcta atcctgcaaa gatagctatt
    17281 tttgcaatta atggtaataa gccgaaaatt ccgccgatta aaatgcttcc tataattaag
    17341 tttcgtttca taatggttcc ctccgatttt ttaatatggt ataatcggaa caaagaatat
    17401 cgggttttat cccaattaag ttgcctttat cctgtccgcc aagactgtta tggataaggc
    17461 tttttctttg ttctcgatca tgttaatgct cctaagttgt ctgcaattca gcagaacgat
    17521 tataaatttt ctcactcact gaaaactgca acccatattt ttctttaaca gtcattaatt
    17581 ctactgtctc atctaaaata ggttcacgat caaccaacat ttcaggagtc atatctttct
    17641 tttttaccat tttggaatga ccataaatag ttgatacagc tttattggct atcacattcg
    17701 ctttaatgta gtctactggt tttggttcag taatagcgtg gcttaattta gtcattgctt
    17761 ctttttggtg ttctttgtct agcatgcgga atacttgaaa gccttcaagt cctgtggact
    17821 ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac
    17881 ggcttttgaa tgccatgcgg taaatatttt ttttcgttga tgatgtttac atcttgtgtt
    17941 ctgcccaatg aatcgatgac cttactagta gtaaccccat ctttaggcaa agagtgaatg
    18001 gctctagtaa cctgtttaag acctaacgct tccgcaacat ctttcgccac tgcccaccat
    18061 tcatcattaa tatcgacaaa tcttataatg tgtccgttcc aaatttcagt tttcattcaa
    18121 cgtcctccag ttcaagccct agcaagctcg ctaacaactc aacttgctga tacaaaattt
    18181 cttgatactc ttcaatagaa agtcgttctt taaactctgc ttgtactaaa tcgttcaagc
    18241 cacgcaatac tccgcttacc gttggttctt gtggtagcaa tttttgatag tccatttgtt
    18301 cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc caagtttgct aattctgccg
    18361 ctgtgtcttc tgtaagaatt gttgataata ctttttcgat tgctttattt tgtgtcgatt
    18421 ccattattat ttcctcctat taatcccttt gcccccacgg cttgcgttta tttttggtat
    18481 tcttccataa agcgatcaca ttcatcttgt gaaattctta cgactccatc aactataatc
    18541 aactttaaac cgttaggaac atatttctgc atcatagtat tgtatgaaac gttcatatac
    18601 ttagcagctt gtgccttgtt catatatttt ggaaactggt actttttttc taataatgaa
    18661 agtaacgttc cttgagtttg ctctacttgt tgaacaactc gatcgtcaat aattttaaaa
    18721 gctggatcat caagcgtgag tttgtcaatt tttaattcca tatttatccc ccctcgttag
    18781 catacatttc tatacaaaga ttttaaaaaa atgcaagagg ctcttgaata catttctata
    18841 cctcttataa actcaatata ccatcatacc ttttcatttg caagaatttt aacatacttt
    18901 tttgaacttt tatgtttcac acatatttaa atcgtgatat agtagagaaa atatatgaat
    18961 ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag tggagacagg acaaagaatt
    19021 agactggcta gaattgaaaa gggactctca atggaacaac ttggaggtaa attatcccca
    19081 ccggccagta aaggtgccgt tagtaattgg gaaaatggct ataatcttcc caataacacc
    19141 aggcttaaac aactttcaaa ggtattagat gtatccacta cctatctatt aaacggatct
    19201 tatacattaa gagatatgca tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa
    19261 ttgaaaaaaa ctattgaaaa aaataactct ataattgatt ctaatctttc taatgctatc
    19321 aataacatcg aagaagataa cctgactgtc tcacagaaga tttatttaac aaacgcaata
    19381 aaatatctta aaacaggatc tcgaattgaa tttcctgacg ggagtacccc aattgcttat
    19441 ctcgcagctc ttctaaatac gcttttacga tatgactcat taattaatga ttcagaaaaa
    19501 aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa atgatcttct aaaagagttc
    19561 acagctcttg ttactgagct aaataaacac tattcaaaat aataataaac tgcctcctcc
    19621 aaccgccccc acggcttgct acactatgga gggaaaacta ttatggcaac attcaagcaa
    19681 tatgaaacta aaaaaggtaa attttggctc tatgaagcct atcttggaat gaacaaaatg
    19741 actggcaaac ctgataaggt tcgtagacga ggttacaaaa caaaaaaaga agcccagctt
    19801 gccttgtcac gcttgcaagt ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa
    19861 acgtttaaag aagtttatga cttgtggcta gaaacatata aaaccactgt ccgagaagcc
    19921 accttcatga aaactgaaat caagtttaga aagtggattc ttccaaaata tggaaatctt
    19981 cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg taaatagctg ggctgaaact
    20041 actgaccaat acaaggtact tcattcaact tctaagcgta tttttagcta cgctatcaat
    20101 cttgggatta ttgagactaa tccactagac aacgtattaa tgcctaaaag aaccaccact
    20161 aagcataaag aaaaggtcaa agtttacagt aaagaacaac tgatcacctt gtttgattac
    20221 ctaaacagca aacctgaaac ttatcgaaac gactttgata aaacgttact acgctttctc
    20281 ttttatagcg gggttaggat cagtgaagcc cttgcattga attggtcaga cattgatttt
    20341 caagaaaata cggtcaccat caataaaaca ctaagccaat ccaaaaatgg ctataaaatc
    20401 agtgatccta aaactgattg tagtgctggg gttcttccac tagatgaaag aacaatttct
    20461 atattaaaaa aatggcagat taatcagcgg aaatatatgc tgacacttgg tgtaactgat
    20521 ccgactatga ttttttgtgg tatctataaa gaactaatca cacaccacgc tatttatgca
    20581 agattgatta ctatttgtga aaaagctatg gttccatttc tcggtgtgca tgtagcaaga
    20641 cacactcatg cttcaatgct cttggacgct ggagctacta tggtagaagt tcaaactaga
    20701 ttacggcata caaaaattag tcagacgatc gattgttacg gtcacttggc aaaagaaacg
    20761 aaagaaaaga ctgttgaaaa attagtacaa cacttaaata ttgactagat ggcatacaat
    20821 atggcataca gtaaaaaata aaaaaaggag aaaccctatc atatcaaggt ttctcctagc
    20881 tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    20941 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    21001 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    21061 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    21121 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    21181 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    21241 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    21301 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    21361 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    21421 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    21481 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    21541 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    21601 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    21661 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    21721 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    21781 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    21841 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    21901 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    21961 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    22021 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    22081 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    22141 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    22201 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    22261 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    22321 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    22381 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    22441 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    22501 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    22561 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    22621 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    22681 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    22741 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    22801 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    22861 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    22921 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    22981 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    23041 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    23101 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    23161 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    23221 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    23281 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    23341 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    23401 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    23461 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    23521 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    23581 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    23641 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    23701 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    23761 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    23821 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    23881 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    23941 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    24001 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    24061 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    24121 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    24181 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    24241 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    24301 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    24361 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    24421 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    24481 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    24541 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    24601 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    24661 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    24721 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    24781 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    24841 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    24901 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    24961 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    25021 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    25081 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    25141 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    25201 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    25261 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    25321 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    25381 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    25441 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    25501 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    25561 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    25621 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    25681 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    25741 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    25801 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    25861 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    25921 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    25981 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    26041 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    26101 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    26161 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    26221 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    26281 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    26341 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    26401 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    26461 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    26521 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    26581 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    26641 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    26701 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    26761 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    26821 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    26881 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    26941 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    27001 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    27061 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    27121 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    27181 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    27241 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    27301 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    27361 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    27421 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    27481 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    27541 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    27601 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    27661 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    27721 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    27781 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    27841 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    27901 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    27961 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    28021 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    28081 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    28141 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    28201 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    28261 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    28321 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    28381 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    28441 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    28501 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    28561 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    28621 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    28681 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    28741 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    28801 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    28861 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    28921 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    28981 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    29041 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    29101 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    29161 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    29221 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    29281 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    29341 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    29401 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    29461 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    29521 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    29581 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    29641 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    29701 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    29761 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    29821 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    29881 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    29941 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    30001 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    30061 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
    30121 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    30181 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    30241 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    30301 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    30361 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    30421 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    30481 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    30541 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    30601 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    30661 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    30721 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    30781 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    30841 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    30901 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    30961 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    31021 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    31081 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    31141 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    31201 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    31261 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    31321 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    31381 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    31441 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    31501 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    31561 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    31621 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    31681 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    31741 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    31801 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    31861 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    31921 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    31981 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    32041 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    32101 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    32161 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    32221 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    32281 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    32341 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    32401 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    32461 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    32521 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    32581 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    32641 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    32701 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    32761 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    32821 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    32881 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    32941 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    33001 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    33061 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    33121 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    33181 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    33241 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    33301 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    33361 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    33421 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    33481 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    33541 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    33601 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    33661 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    33721 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    33781 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    33841 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    33901 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    33961 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    34021 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    34081 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    34141 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    34201 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    34261 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    34321 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    34381 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    34441 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    34501 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    34561 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    34621 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    34681 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    34741 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    34801 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    34861 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    34921 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    34981 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    35041 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    35101 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    35161 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    35221 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    35281 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    35341 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    35401 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    35461 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    35521 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    35581 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    35641 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    35701 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    35761 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    35821 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    35881 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    35941 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    36001 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    36061 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    36121 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    36181 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    36241 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    36301 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    36361 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    36421 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    36481 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    36541 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    36601 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    36661 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    36721 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    36781 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    36841 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    36901 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    36961 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    37021 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    37081 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    37141 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    37201 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    37261 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    37321 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    37381 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    37441 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    37501 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    37561 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    37621 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    37681 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    37741 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    37801 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    37861 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    37921 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    37981 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    38041 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    38101 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    38161 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    38221 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    38281 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    38341 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    38401 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    38461 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    38521 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    38581 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    38641 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    38701 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    38761 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    38821 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    38881 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    38941 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    39001 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    39061 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    39121 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    39181 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    39241 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    39301 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    39361 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    39421 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    39481 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    39541 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    39601 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    39661 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    39721 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    39781 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    39841 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    39901 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    39961 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    40021 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    40081 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    40141 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    40201 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    40261 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    40321 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    40381 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    40441 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    40501 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    40561 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    40621 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    40681 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    40741 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    40801 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    40861 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    40921 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    40981 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    41041 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    41101 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    41161 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    41221 ttagtaacac aaatatttat ataaaaaaat attttacttg
//
LOCUS       DASJOX010000013        39475 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJOX010000013 DASJOX010000000
VERSION     DASJOX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.013"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..878
                     /locus_tag="U4A12_000661"
     CDS             21..878
                     /locus_tag="U4A12_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN1773070.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            993..1913
                     /gene="pstC"
                     /locus_tag="U4A12_000662"
     CDS             993..1913
                     /gene="pstC"
                     /locus_tag="U4A12_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN1773071.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1913..2797
                     /gene="pstA"
                     /locus_tag="U4A12_000663"
     CDS             1913..2797
                     /gene="pstA"
                     /locus_tag="U4A12_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN1773072.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2812..3618
                     /gene="pstB"
                     /locus_tag="U4A12_000664"
     CDS             2812..3618
                     /gene="pstB"
                     /locus_tag="U4A12_000664"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1773073.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3634..4392
                     /locus_tag="U4A12_000665"
     CDS             3634..4392
                     /locus_tag="U4A12_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1773074.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4405..5082
                     /gene="phoU"
                     /locus_tag="U4A12_000666"
     CDS             4405..5082
                     /gene="phoU"
                     /locus_tag="U4A12_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN1773075.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5320..6351
                     /locus_tag="U4A12_000667"
     CDS             5320..6351
                     /locus_tag="U4A12_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1773076.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6348..7115
                     /locus_tag="U4A12_000668"
     CDS             6348..7115
                     /locus_tag="U4A12_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1773077.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7215..8783
                     /gene="liaX"
                     /locus_tag="U4A12_000669"
     CDS             7215..8783
                     /gene="liaX"
                     /locus_tag="U4A12_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN1773078.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9080..9400
                     /locus_tag="U4A12_000670"
     CDS             9080..9400
                     /locus_tag="U4A12_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN1773079.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9400..9750
                     /locus_tag="U4A12_000671"
     CDS             9400..9750
                     /locus_tag="U4A12_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1773080.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9917..10855
                     /locus_tag="U4A12_000672"
     CDS             9917..10855
                     /locus_tag="U4A12_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN1773081.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10870..11700
                     /locus_tag="U4A12_000673"
     CDS             10870..11700
                     /locus_tag="U4A12_000673"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1773082.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11744..12769
                     /locus_tag="U4A12_000674"
     CDS             11744..12769
                     /locus_tag="U4A12_000674"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1773083.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12784..13722
                     /gene="galU"
                     /locus_tag="U4A12_000675"
     CDS             12784..13722
                     /gene="galU"
                     /locus_tag="U4A12_000675"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1773084.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13847..14287
                     /locus_tag="U4A12_000676"
     CDS             13847..14287
                     /locus_tag="U4A12_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN1773085.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14290..14892
                     /locus_tag="U4A12_000677"
     CDS             14290..14892
                     /locus_tag="U4A12_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1773086.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14951..16057)
                     /locus_tag="U4A12_000678"
     CDS             complement(14951..16057)
                     /locus_tag="U4A12_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1773087.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16267..17268
                     /gene="ccpA"
                     /locus_tag="U4A12_000679"
     CDS             16267..17268
                     /gene="ccpA"
                     /locus_tag="U4A12_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN1773088.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17605..19950)
                     /locus_tag="U4A12_000680"
     CDS             complement(17605..19950)
                     /locus_tag="U4A12_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1773089.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20229..21131
                     /locus_tag="U4A12_000681"
     CDS             20229..21131
                     /locus_tag="U4A12_000681"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN1773090.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21344..23119
                     /locus_tag="U4A12_000682"
     CDS             21344..23119
                     /locus_tag="U4A12_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN1773091.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23185..24771)
                     /locus_tag="U4A12_000683"
     CDS             complement(23185..24771)
                     /locus_tag="U4A12_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN1773092.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25022..25246
                     /locus_tag="U4A12_000684"
     CDS             25022..25246
                     /locus_tag="U4A12_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN1773093.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25388..27139
                     /locus_tag="U4A12_000685"
     CDS             25388..27139
                     /locus_tag="U4A12_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1773094.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27139..28923
                     /locus_tag="U4A12_000686"
     CDS             27139..28923
                     /locus_tag="U4A12_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773095.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29249..30100
                     /locus_tag="U4A12_000687"
     CDS             29249..30100
                     /locus_tag="U4A12_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1773096.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30154..31905
                     /locus_tag="U4A12_000688"
     CDS             30154..31905
                     /locus_tag="U4A12_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1773097.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            31909..32079
                     /locus_tag="U4A12_000689"
     CDS             31909..32079
                     /locus_tag="U4A12_000689"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1773098.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(32119..33021)
                     /locus_tag="U4A12_000690"
     CDS             complement(32119..33021)
                     /locus_tag="U4A12_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1773099.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33252..34655
                     /locus_tag="U4A12_000691"
     CDS             33252..34655
                     /locus_tag="U4A12_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1773100.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34655..36088
                     /locus_tag="U4A12_000692"
     CDS             34655..36088
                     /locus_tag="U4A12_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1773101.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            36233..37159
                     /gene="trxB"
                     /locus_tag="U4A12_000693"
     CDS             36233..37159
                     /gene="trxB"
                     /locus_tag="U4A12_000693"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN1773102.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37373..39100
                     /locus_tag="U4A12_000694"
     CDS             37373..39100
                     /locus_tag="U4A12_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1773103.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39115..39465
                     /locus_tag="U4A12_000695"
     CDS             39115..39465
                     /locus_tag="U4A12_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1773104.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        12656 a         6970 c         8462 g        11387 t
ORIGIN      
        1 aggattaaag gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag
       61 ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg
      121 gctcttctgc tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc
      181 ctggaaaatt catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt
      241 caggagcagt cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg
      301 cctcagcatt agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg
      361 atattggcgt aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca
      421 aaaactggag tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag
      481 gaagcggaac ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg
      541 cacaagaaca agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa
      601 tcagctatgt tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg
      661 tagaaccgac agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata
      721 tgtacacaag agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg
      781 atgaagtaca agataacatc gttggagaac taggttatat ccctgtttct catatgaaag
      841 tcgaacgtga ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac
      901 ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga
      961 gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa
     1021 caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg
     1081 cgctgatcgt gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca
     1141 ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa
     1201 gccaaatcgg agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt
     1261 ttattgtgac ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat
     1321 tcatgacgga gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc
     1381 ttgtaggtat tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg
     1441 tcagaagtat ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg
     1501 ttatgatctt gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc
     1561 actatcggga agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg
     1621 tgctacgagc agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt
     1681 ttggtgaagc cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc
     1741 ttactactcc tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca
     1801 tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat
     1861 tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc
     1921 aaaacgctca gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt
     1981 gattttagca gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc
     2041 cttcttgacc gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt
     2101 taactcgatt tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc
     2161 gggtatctat ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc
     2221 gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt
     2281 cgttattcaa ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt
     2341 caacttgcct cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca
     2401 acgagaagca ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt
     2461 tccagaagcg acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg
     2521 tgaagcagct gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa
     2581 ctggaatcca ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc
     2641 tgtccatatt tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc
     2701 tggcgcttct gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat
     2761 tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa
     2821 tataactgga atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat
     2881 acggatgatc tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa
     2941 tttgaaaaaa ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat
     3001 cttcgttcgt tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc
     3061 atgtatcaag gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg
     3121 attggcatgg tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca
     3181 tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca
     3241 agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg
     3301 gctttatctg gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct
     3361 gatatcttgc tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt
     3421 gaagaaacac ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg
     3481 caacaagcag ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa
     3541 tatgacaaaa ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg
     3601 tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa
     3661 gatcttcatc tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat
     3721 caaggggaaa tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt
     3781 tctttgaacc ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat
     3841 aaagacaaag acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc
     3901 atggtattcc agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga
     3961 ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg
     4021 aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta
     4081 tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc
     4141 atcttattgg atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt
     4201 atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa
     4261 gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat
     4321 gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt
     4381 cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat
     4441 ctccataatc agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt
     4501 cgtgcataca tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat
     4561 atcaataaca tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag
     4621 ccagttacaa ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa
     4681 cgtatggccg atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg
     4741 agaataccgg aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg
     4801 gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat
     4861 atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg
     4921 caggaaaatc ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta
     4981 gaacggatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg
     5041 aaaatcactg agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg
     5101 ccgtttgttt aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca
     5161 agtaataaga acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat
     5221 taccgcagat gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg
     5281 tcacaaaccc ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc
     5341 atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta
     5401 tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt
     5461 acaggaaaac tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat
     5521 caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg
     5581 catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca
     5641 agtatcttca ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga
     5701 agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa
     5761 gggatttctc tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata
     5821 ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt
     5881 gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg
     5941 cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta
     6001 ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa
     6061 gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa
     6121 gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg
     6181 tcagttccat cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa
     6241 cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg
     6301 atccctatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta
     6361 ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt
     6421 atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc
     6481 atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta
     6541 tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga
     6601 tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt
     6661 ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg attgatttta
     6721 gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg
     6781 tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc
     6841 aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac
     6901 ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca
     6961 ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc
     7021 ttaccacatt gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct
     7081 ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca
     7141 tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta
     7201 aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc
     7261 tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc
     7321 aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc
     7381 aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg
     7441 aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag
     7501 cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag
     7561 cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt
     7621 tgacagaaga agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag
     7681 ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc
     7741 gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg
     7801 attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg
     7861 gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg
     7921 acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg
     7981 agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg
     8041 gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac
     8101 tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg
     8161 ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt
     8221 tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca
     8281 tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg
     8341 tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc
     8401 gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg
     8461 cgacagcaga aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag
     8521 aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac
     8581 cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc
     8641 tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc
     8701 gtcgtgtttc agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct
     8761 atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa
     8821 gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt
     8881 ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca
     8941 aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa
     9001 ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat
     9061 agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt
     9121 tttgacaggg actttagcag gaataggaga atacatcggg attgatccga ctgttatccg
     9181 tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt
     9241 attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc
     9301 atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga
     9361 agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt
     9421 ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg
     9481 agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc
     9541 aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg
     9601 tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt
     9661 agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta
     9721 attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt
     9781 ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt
     9841 tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag
     9901 aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc
     9961 tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat
    10021 ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca
    10081 gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat
    10141 catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc
    10201 agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc
    10261 catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc
    10321 tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg
    10381 tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt
    10441 gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc
    10501 gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa
    10561 tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga
    10621 agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat
    10681 cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat
    10741 catcattgaa gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa
    10801 aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg
    10861 agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc
    10921 tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga
    10981 gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg
    11041 cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata
    11101 gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg
    11161 attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact
    11221 tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat
    11281 ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat
    11341 ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt
    11401 ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa
    11461 ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg
    11521 cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt
    11581 tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga
    11641 aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga
    11701 tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc
    11761 ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca
    11821 tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac
    11881 gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat
    11941 ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg
    12001 ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag
    12061 taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc
    12121 agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc
    12181 aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt
    12241 acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt
    12301 agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt
    12361 agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg
    12421 tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga
    12481 tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg
    12541 acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa
    12601 tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca
    12661 aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat
    12721 gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg
    12781 tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca
    12841 gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc
    12901 atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct
    12961 aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa
    13021 aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc
    13081 atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt
    13141 ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg
    13201 acacagcaat taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa
    13261 gttcctcatg atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat
    13321 gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat
    13381 ttagctatta ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa
    13441 caaccaggag ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca
    13501 caacgcgtat ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt
    13561 tttatgaaaa caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt
    13621 gagtacatcc tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat
    13681 ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc
    13741 ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg
    13801 aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga
    13861 aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt
    13921 gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac
    13981 gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact
    14041 ggtcaaaagt aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt
    14101 gttcacagca gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa
    14161 catcgcgacg aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa
    14221 agtcggtggg acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg
    14281 aaaataaaaa tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc
    14341 gcagcaatcg ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta
    14401 gctgcccaag cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa
    14461 aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca
    14521 ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt
    14581 gaatctgacg atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc
    14641 aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat
    14701 acttcagaag atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc
    14761 aatgaaggaa tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg
    14821 gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct
    14881 acaaatcact aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc
    14941 ttttttactc ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac
    15001 agccatcgct tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat
    15061 agattcctgg ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta
    15121 caagggaagg gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat
    15181 attcgccgta gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg
    15241 tcacacctgg acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga
    15301 ttttttcttg gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat
    15361 agccattcca aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat
    15421 ttcctggtgt accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag
    15481 acatttgtga aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt
    15541 cagtcacgcc ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac
    15601 cagcttcgat cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga
    15661 tggaagttag atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc
    15721 gctcgacagt caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac
    15781 gaagttcttt tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat
    15841 acgcccaact actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat
    15901 cttgttcaag agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg
    15961 aaaagtaagc gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca
    16021 tccattgacg tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta
    16081 attttgtatt caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa
    16141 aaaagtaaaa attttcatga aaacagagat aaaacggttg caattcttga gattatttga
    16201 tattctattt tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga
    16261 acacaaatgg aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg
    16321 atggctactg tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa
    16381 aaagtcttgg aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg
    16441 gcaagtaaga aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt
    16501 gcttctttag cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta
    16561 gcaaattcag atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag
    16621 caagtggacg gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt
    16681 tcacgttcaa aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt
    16741 agcgtaaaca ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc
    16801 ggcaataaaa aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat
    16861 cgtctgaaag gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg
    16921 atctttgaag cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat
    16981 agcggtgcaa cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt
    17041 ttattagacc gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca
    17101 ctactgacag aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta
    17161 ggggcggtat ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa
    17221 acgattatct tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac
    17281 cattcagagg ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac
    17341 agcatctcat gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt
    17401 ttatttggga aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa
    17461 aagagagaaa gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct
    17521 gcattttgtt caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc
    17581 cttattactg tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg
    17641 attcgctctt gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc
    17701 gccaacgcca aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac
    17761 tgtttttcct ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc
    17821 agtaaatcca gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt
    17881 gccaaaaatg tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc
    17941 cgaacgtatc cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact
    18001 gatggtaata gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc
    18061 agtttttccg acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt
    18121 gaatggtgta gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc
    18181 cgttgcttcc gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc
    18241 gttatctgtg atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc
    18301 ctgaaatccg ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc
    18361 aacgccccaa ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata
    18421 ttgtttcgat ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt
    18481 ggaccattct agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt
    18541 gacgattttc tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg
    18601 ggattctgat ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat
    18661 tgatgagcca gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt
    18721 ccgtccgcca acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt
    18781 ttctacttga ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg
    18841 caatgcagca tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat
    18901 cgtttgctgt gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt
    18961 gtctttttca gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata
    19021 caagaattct ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc
    19081 ataggctttt gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa
    19141 taaaacaaaa tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt
    19201 cgtgtatgga ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg
    19261 cagcgtcaca tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc
    19321 aatgttttgt cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat
    19381 ctcatctttt gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg
    19441 tttgaatgtt gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga
    19501 tccaccagaa gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg
    19561 aacgtaacct tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa
    19621 taaaggtgaa atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga
    19681 tatctctgta ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt
    19741 ctgcagtgct tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc
    19801 gcctattcca atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa
    19861 tgattgaaaa actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt
    19921 ctgttttttt cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc
    19981 gctaattata gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc
    20041 ttttttaaaa gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt
    20101 ttctttagag aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc
    20161 tttgtatatc atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga
    20221 atccatccat gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag
    20281 cagcagaaga agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc
    20341 aaaacactag aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg
    20401 cagaaaaagg tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata
    20461 cgatcaaacc agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg
    20521 cggaagcttt aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa
    20581 cagaagccgg aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga
    20641 cggcacaaat cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg
    20701 aggaattagc gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg
    20761 acacctgtca tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg
    20821 aagagtttga taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta
    20881 aaaatccgca aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat
    20941 tcgacacgct gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg
    21001 aaactcccta tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg
    21061 ccatgttcaa agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag
    21121 tgatactcta agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc
    21181 cttttttgtt taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc
    21241 ccccctcttt ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa
    21301 gtttcacaag caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa
    21361 acccttgatc aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt
    21421 atttacaatt attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc
    21481 aacctatgat gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct
    21541 aggggagtat acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact
    21601 tgcacataaa ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag
    21661 tatattggtt gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat
    21721 ctatttttat tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata
    21781 tcgtaatgcc ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta
    21841 ttaccgcaaa tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc
    21901 ttttcctttc ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt
    21961 agctttgggt atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt
    22021 aaagaagtta aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt
    22081 gtttttactt cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga
    22141 gacaacatac ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat
    22201 gaaacaactg atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg
    22261 ggtctcacat gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc
    22321 tacccgtatt gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga
    22381 attacccgga gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta
    22441 ccaaaacaat gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga
    22501 agcctatcac aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga
    22561 tggaaagacg atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta
    22621 tgcatggcga tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca
    22681 agtcaaacag gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa
    22741 tgaaccgaga acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat
    22801 aaaaatttac caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact
    22861 tatgatacta ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat
    22921 tgtggtcacc ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg
    22981 ttcttatgcg ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc
    23041 attaacgcaa ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa
    23101 agctttatct tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg
    23161 gtctttgtgt ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg
    23221 ttcatatttt ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac
    23281 aagaacaatg attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg
    23341 ctcgccgaag aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact
    23401 aatcacagag aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat
    23461 ctccgcttgg ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc
    23521 caagaacacc tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc
    23581 ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac
    23641 cagtcttctt agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc
    23701 ggtcaacgta ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa
    23761 agtggacaag ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg
    23821 atcagataaa gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa
    23881 gacagcgaca cccatgatca atagcagtgc attgactaga aaagccattg acaactggat
    23941 attagaatcc caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc
    24001 gatcttccga gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc
    24061 accaataatc cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga
    24121 gaatgtatga ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa
    24181 gccaacttga tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc
    24241 gatcttacga aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat
    24301 aaagacggca atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat
    24361 gacttctgca atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc
    24421 taatgtctta cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc
    24481 taattttgct gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga
    24541 catcaataaa tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc
    24601 catataccca actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa
    24661 acttttcccc tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt
    24721 atgttcaata agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc
    24781 acctttccta tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa
    24841 gattcggtac gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg
    24901 ttctcctcta aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt
    24961 caataaactt tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta
    25021 tatgttatca acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt
    25081 aggtggcttt ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt
    25141 taatgaggac atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa
    25201 aattcgccag atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg
    25261 actttttacg ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca
    25321 gattattcag caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag
    25381 gagtaaaatg tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta
    25441 tttaattggg gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt
    25501 gattgggatc atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg
    25561 gtggatcttc ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg
    25621 gacgaatatt tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa
    25681 acattttaca gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc
    25741 tcacgcaacg aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt
    25801 tgcagattca gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg
    25861 gcgcttaaca ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct
    25921 ggggtcaaaa ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga
    25981 taagacacaa gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga
    26041 agatctagcg gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa
    26101 ttttttagac tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc
    26161 aacaattatt ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt
    26221 ctcatttatt agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt
    26281 caacgtattg gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa
    26341 gacgcatatc gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt
    26401 agctatttcc tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt
    26461 tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac
    26521 gatcatcaaa ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg
    26581 acgttcgatc aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca
    26641 ggatcacttc ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact
    26701 ttcagaagaa gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga
    26761 attaccaaaa ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca
    26821 gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga
    26881 tgatgcactc tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga
    26941 aataagaaaa gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc
    27001 aaaagaaatc attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct
    27061 gcttgcacta ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat
    27121 agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca
    27181 cttaaagaac agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga
    27241 acgttcatcg ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca
    27301 aggatcatcc aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt
    27361 attttgtttt ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt
    27421 ttccaatggt atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa
    27481 ttatttgaaa agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca
    27541 accgtttctc gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg
    27601 atggtattga ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat
    27661 ggagaaattg ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat
    27721 taccagaaat tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac
    27781 acgaaattga atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct
    27841 cgtttagcta aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg
    27901 atcaaaacga attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt
    27961 gcgttggtgt tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg
    28021 atctatgcat ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac
    28081 tttttgagca tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat
    28141 acggaagaat atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag
    28201 attgagtttc gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc
    28261 tcttttgttg ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa
    28321 agctcgatca tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt
    28381 gatgatcatg acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc
    28441 ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca
    28501 gctatcacag acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc
    28561 gatgaattac caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt
    28621 ggacaacgac agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta
    28681 ttggatgaag caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg
    28741 gcaaaaatgc gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga
    28801 gatgcagaat tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa
    28861 acacttctag cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata
    28921 taaaagatgt tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc
    28981 ttctcatatt tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc
    29041 gacagctagt cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt
    29101 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    29161 atgagtctcc catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag
    29221 ccctatacat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    29281 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    29341 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    29401 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    29461 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    29521 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    29581 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    29641 catcgggaaa aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga
    29701 tgaagccgga tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga
    29761 caatacctac ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta
    29821 taagattaca tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt
    29881 ttttctgaca cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt
    29941 gtttcaggtt attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga
    30001 ctttttagcc gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat
    30061 ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac
    30121 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    30181 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    30241 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    30301 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    30361 cgagcgggag ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag
    30421 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    30481 tttgatatgc ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct
    30541 gctggcggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    30601 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    30661 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    30721 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    30781 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    30841 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    30901 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    30961 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    31021 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    31081 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    31141 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    31201 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    31261 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    31321 attgtgggga ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt
    31381 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    31441 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    31501 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    31561 acgctgactt ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa
    31621 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca
    31681 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    31741 ggagaagtac gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca
    31801 attggaatgg tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg
    31861 cagttggaag ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc
    31921 gatctgttgc tacgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca
    31981 cctgtgatcg tcaccaatta cacggattat ttggatattt tggaaaacca aggggagacg
    32041 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aagaatggac
    32101 taggcttaac tattcttttt attccacttc tttattttca aaattgaaag gctctttcat
    32161 gatggctgag ctgatagggc gtttatcgaa tttttgaccg acagaaatta gatggactaa
    32221 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    32281 tgatgtattg atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg
    32341 caaagcggct gtactcattg cagatagcgt tgaatcaccg atactggtca aagctaaaat
    32401 tggaacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    32461 ataagaaatc agaatcccgc acatatcact cgtgcaattg tacgcttcat agaatgattc
    32521 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    32581 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    32641 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat catgaatcgt
    32701 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    32761 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    32821 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    32881 agaggggtgg acataacatc gctcagcaat cgcttggatg gtcatttcgc caagtgctgg
    32941 agaatcgctt aagagaaact ggatcacttc attttctgaa ggagaaaagg agatttcttt
    33001 gattttttca cgtaataaca tgtagatacc ttcttcttat ttttttacgt aaattataac
    33061 ttttttgaaa atgatttgta aacggattta tactatataa cttaaaatag acattttttg
    33121 acaagatatt tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga
    33181 aacagatttc tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa
    33241 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    33301 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    33361 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc
    33421 gtatgtggga tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag
    33481 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    33541 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    33601 gcgggatatt caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga
    33661 agcgtttatg tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag
    33721 aagcagttat cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga
    33781 tggcgatgat gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt
    33841 ttgccggtct aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca
    33901 cggcagaatt gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg
    33961 atatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    34021 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    34081 gtacaagagc gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc
    34141 cagaattaga ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg
    34201 ctgcctgtga aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta
    34261 caaagaatga tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct
    34321 atgttggcgc acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag
    34381 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg
    34441 attatgggac agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag
    34501 caaaacggat catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg
    34561 agacaatcag ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat
    34621 tatcaggtaa tacacatata atggaggaag tataatggga ttcagaaaag attttttatg
    34681 gggcggtgct acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact
    34741 agcaaacgta gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa
    34801 aaaaatgttt gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta
    34861 tcatcgttat aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat
    34921 gtccatcgcc tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg
    34981 cttgaaattt tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt
    35041 gacgattacg cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc
    35101 cagagaactc gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg
    35161 acttgtcaaa tattggctga cattcaatga gatcaatatg atccttcatg caccattcat
    35221 gggcgcaggg ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc
    35281 acatcatgag ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga
    35341 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    35401 agatgtttgg gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc
    35461 acgaggagaa tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga
    35521 aatggaacca ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc
    35581 ttattatgct tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa
    35641 tatctttgct tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga
    35701 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt
    35761 tattgtagaa aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga
    35821 tgattaccga atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact
    35881 agacggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    35941 taccggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa
    36001 tggcacatta gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc
    36061 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    36121 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    36181 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    36241 tgtaatcgtt attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc
    36301 aaacttatcc gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc
    36361 agaaatcgaa aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat
    36421 gtatgaaagt gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa
    36481 ggatcacggc agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt
    36541 cattatcgca acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    36601 tggacgaggc gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct
    36661 agtagtcatc gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc
    36721 ttctgaagta gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    36781 ccgtgcattt gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    36841 tggaaacgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt
    36901 tgatttagca gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt
    36961 tagagaagca ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac
    37021 gaatatgcca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    37081 tacagctgtt ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt
    37141 agaagaaaca acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac
    37201 tctttttttt cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt
    37261 gataaaagaa tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata
    37321 agtgaatgct atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg
    37381 ggaacaagtg tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca
    37441 attaaatgat ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga
    37501 attcggtact gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta
    37561 tacggtaaga caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac
    37621 aaaacgccga ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat
    37681 ggaagcagcc aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg
    37741 tccaacacca gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt
    37801 aaccgcatct cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg
    37861 acaaatgcct cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc
    37921 gctaaaagtc gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat
    37981 cggagaagaa gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga
    38041 attggttgaa acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg
    38101 gaaaatgttg ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc
    38161 cgaacaagct gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca
    38221 ttcagctttt gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc
    38281 aacagatcct gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga
    38341 agtattgact gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca
    38401 atcaagaggc actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt
    38461 gcctacagca attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa
    38521 attcattgct gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg
    38581 attcgaagaa agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca
    38641 agcattgctt ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta
    38701 tgacggcttg caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt
    38761 gacaatgagc ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa
    38821 agaagcaccg acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt
    38881 gacgagaaca gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt
    38941 gaagtataca ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa
    39001 aatcaagttt tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc
    39061 ctcttatgaa gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa
    39121 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    39181 acgcaaaaat caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact
    39241 cgtcttaaaa ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    39301 gcagtggaaa tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat
    39361 gtcgtcaagt ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta
    39421 ctggatattt tgaaccagac actcaaacat gtggaacatc aataacgata ggtga
//
LOCUS       DASJOX010000014        38240 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJOX010000014 DASJOX010000000
VERSION     DASJOX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.014"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..944)
                     /locus_tag="U4A12_000696"
     CDS             complement(69..944)
                     /locus_tag="U4A12_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1773105.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1053..2822)
                     /gene="aspS"
                     /locus_tag="U4A12_000697"
     CDS             complement(1053..2822)
                     /gene="aspS"
                     /locus_tag="U4A12_000697"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN1773106.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2837..4129)
                     /locus_tag="U4A12_000698"
     CDS             complement(2837..4129)
                     /locus_tag="U4A12_000698"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN1773107.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4523..5470
                     /locus_tag="U4A12_000699"
     CDS             4523..5470
                     /locus_tag="U4A12_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1773108.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5559..6818)
                     /locus_tag="U4A12_000700"
     CDS             complement(5559..6818)
                     /locus_tag="U4A12_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN1773109.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(6989..7435)
                     /locus_tag="U4A12_000701"
     CDS             complement(6989..7435)
                     /locus_tag="U4A12_000701"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN1773110.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7446..9659)
                     /locus_tag="U4A12_000702"
     CDS             complement(7446..9659)
                     /locus_tag="U4A12_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN1773111.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9892..10656)
                     /locus_tag="U4A12_000703"
     CDS             complement(9892..10656)
                     /locus_tag="U4A12_000703"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN1773112.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10657..11604)
                     /gene="prmA"
                     /locus_tag="U4A12_000704"
     CDS             complement(10657..11604)
                     /gene="prmA"
                     /locus_tag="U4A12_000704"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN1773113.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11617..12105)
                     /locus_tag="U4A12_000705"
     CDS             complement(11617..12105)
                     /locus_tag="U4A12_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN1773114.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12071..12742)
                     /locus_tag="U4A12_000706"
     CDS             complement(12071..12742)
                     /locus_tag="U4A12_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN1773115.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12880..14166
                     /locus_tag="U4A12_000707"
     CDS             12880..14166
                     /locus_tag="U4A12_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN1773116.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14238..14432
                     /gene="ssrS"
                     /locus_tag="U4A12_000708"
     ncRNA           14238..14432
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4A12_000708"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14510..14794
                     /locus_tag="U4A12_000709"
     CDS             14510..14794
                     /locus_tag="U4A12_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773117.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14867..15346
                     /locus_tag="U4A12_000710"
     CDS             14867..15346
                     /locus_tag="U4A12_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1773118.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15568..15711
                     /locus_tag="U4A12_000711"
     CDS             15568..15711
                     /locus_tag="U4A12_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN1773119.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16289..17872
                     /locus_tag="U4A12_000712"
     CDS             16289..17872
                     /locus_tag="U4A12_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1773120.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18309..19493)
                     /locus_tag="U4A12_000713"
     CDS             complement(18309..19493)
                     /locus_tag="U4A12_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN1773121.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19518..20525)
                     /locus_tag="U4A12_000714"
     CDS             complement(19518..20525)
                     /locus_tag="U4A12_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1773122.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20697..21056)
                     /locus_tag="U4A12_000715"
     CDS             complement(20697..21056)
                     /locus_tag="U4A12_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773123.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21028..21399)
                     /locus_tag="U4A12_000716"
     CDS             complement(21028..21399)
                     /locus_tag="U4A12_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN1773124.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21416..21718)
                     /locus_tag="U4A12_000717"
     CDS             complement(21416..21718)
                     /locus_tag="U4A12_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773125.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21735..22205)
                     /locus_tag="U4A12_000718"
     CDS             complement(21735..22205)
                     /locus_tag="U4A12_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN1773126.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22180..22458)
                     /locus_tag="U4A12_000719"
     CDS             complement(22180..22458)
                     /locus_tag="U4A12_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN1773127.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22455..23504)
                     /locus_tag="U4A12_000720"
     CDS             complement(22455..23504)
                     /locus_tag="U4A12_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN1773128.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23461..24390)
                     /gene="tadA"
                     /locus_tag="U4A12_000721"
     CDS             complement(23461..24390)
                     /gene="tadA"
                     /locus_tag="U4A12_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN1773129.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24522..26276
                     /gene="ade"
                     /locus_tag="U4A12_000722"
     CDS             24522..26276
                     /gene="ade"
                     /locus_tag="U4A12_000722"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN1773130.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26374..27036)
                     /gene="trmB"
                     /locus_tag="U4A12_000723"
     CDS             complement(26374..27036)
                     /gene="trmB"
                     /locus_tag="U4A12_000723"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN1773131.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27106..27888)
                     /locus_tag="U4A12_000724"
     CDS             complement(27106..27888)
                     /locus_tag="U4A12_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN1773132.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28092..29303)
                     /locus_tag="U4A12_000725"
     CDS             complement(28092..29303)
                     /locus_tag="U4A12_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773133.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29296..30033)
                     /locus_tag="U4A12_000726"
     CDS             complement(29296..30033)
                     /locus_tag="U4A12_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773134.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30171..30605
                     /locus_tag="U4A12_000727"
     CDS             30171..30605
                     /locus_tag="U4A12_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN1773135.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30598..30942
                     /locus_tag="U4A12_000728"
     CDS             30598..30942
                     /locus_tag="U4A12_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1773136.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31122..32132
                     /locus_tag="U4A12_000729"
     CDS             31122..32132
                     /locus_tag="U4A12_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1773137.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32201..33145)
                     /locus_tag="U4A12_000730"
     CDS             complement(32201..33145)
                     /locus_tag="U4A12_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1773138.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33129..35849)
                     /locus_tag="U4A12_000731"
     CDS             complement(33129..35849)
                     /locus_tag="U4A12_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1773139.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35846..37066)
                     /locus_tag="U4A12_000732"
     CDS             complement(35846..37066)
                     /locus_tag="U4A12_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN1773140.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(37347..38192)
                     /locus_tag="U4A12_000733"
     CDS             complement(37347..38192)
                     /locus_tag="U4A12_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1773141.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        11284 a         7930 c         6693 g        12333 t
ORIGIN      
        1 agaaaaaaac tgcagacaaa cgaacgtctg tctacagcag tatttcatga atttttgtca
       61 aactcgatct aaatgccatc ctcatagaaa cgaccaagta tctggacgtt ttctgcaata
      121 cttacaaaag cttgtggatc agaagctttc attgcttctc taagtagagg cagttcgtat
      181 cgtgtaacga tcgtcatcaa tactgtctgc ttatcatgct tataagcacc ttctgcttca
      241 ttgatgatcg tgatccctct acgcatacag ttttggattt catttactac tttttctgga
      301 tgtttcgtta cgatcattac ctgcatcttc ttttgtttag tataaacgac atcagttact
      361 tttccactga cgaaaataga caacgtactg taaaacatat attgccagcc aaatagaatt
      421 ccggcaacaa aaacgatcaa tgcattgaag tagatcgaaa tagaaccaat cgactttcct
      481 gtttttttac gaatcgtgat actcacgata tctagaccgc ccgatgatag gccgtttctc
      541 aacgctagcc caatacctag tcccatcaca gcccccccaa acaacgcaca gatgattgga
      601 tcggtagata ggactgtttc cggtaaaata tggatacaaa gggaggtcaa agtgactgtg
      661 ataaaagtga aaattgtaaa cttcttcccg atttttaacc acgccaatac aaacaaaggt
      721 atattcagta aatacaaagt tgttgatacc ggtacttcga atcctatcag tgattttgac
      781 aaagtcgtca aaatctgagc tagaccagta ataccacttg aataaatatg tcctggctga
      841 taaaagaaat tcaatgcgac tgatgcgatc aacgcataaa cgatagatac cgaaagcttg
      901 gtcgtgtaat catgataagc ccaattttcg aatgtttttt tcatggaaaa gccccgcttt
      961 atatgttatg gcttcattat aaagtgtttt tcaattaaat caaggttgtc aaaagaagcg
     1021 tattcgacaa atccttttta cctttttatc ggctattctt ctatattcgt tacatcaata
     1081 ttcaattcga acaattgtaa aggcgaaacc atacttggtg catcactcat cacatctgct
     1141 gcttttccgt ttttaggaaa ggcaatgact tcacgaatat tatcttctcc ggctaataac
     1201 attgcaaaac ggtccaatcc taatgcaata ccgccatgtg gagggaatcc gtaatctaaa
     1261 gctgttaaca agaaaccaaa ttgttcttcc atttgttctt ttgtaaagcc tagagtttct
     1321 agtacttttt cttgaagctc acgagtatga atacggatag aacctccgcc taattcataa
     1381 ccgttcaaga cgatatcata agcttctgca tagacttttg cagggtcttc agccaataac
     1441 gggatatctt cttcttttgg catcgtgaat ggatggtgag cagagacata tcgaccttct
     1501 tcttctgaaa attcaaattg tggccaatca gttacccata agaaattgaa tttcgattca
     1561 tcaattagtc ctaattcttt tcctaaacgt gtccggatag cacctaatgc agcagcgacg
     1621 atttcacttt tatctgcgcc aaacatcagc aagtcacctg gttttgcatc tgttgcttta
     1681 atgattgctt ctgttgcttc tcccatgaat ttagcaattg gacctttcaa tccgtcttct
     1741 tctactttca gccatgcaag ccccttagct ccgaattgtc ctacatattg acctaactga
     1801 tccatgtctt ttcgtgagta gcgatctgca gcgcctttag cattcaatgc tttcacgaca
     1861 ccgccgtttt caagagccat ttggaagact ttgaattcta cttctttgac tgtatcgctt
     1921 agatcgatca attccatatc aaaacgtgta tccggtttgt cgctgccgta gcgagccatt
     1981 gcttcatcat aggtcatacg aggaaatggc aatgtgactt cgataccgcg aacttctttc
     2041 attaccttcg caatcaatcc ctctgtgtaa gtttggattt cttctgctgt taagaacgtt
     2101 gtttcaatat ctacttgcgt aaattcaggc tgacggtctc cacgtaaatc ttcatcgcgg
     2161 aaacaacgaa cgatttgata ataacggtcg aatccagcat tcatcagcaa ttgtttaaac
     2221 aattgaggag attgcggcaa cgcatagaaa tgtccagcat ggacacgtga aggaactagg
     2281 tagtctcttg cgccttctgg tgtagatttc cctaggtaag gtgtttcgat atcgagaaag
     2341 tcattatcat ctaaataatg acgaatcgct tttgttgtcg catttcttaa tttgatgttt
     2401 ttagtcattt ttggacgacg cagatccaaa taacgatatt tcaaacgtat ttcgtcactg
     2461 attgcttgat catcttcaat ttgaaatggc ggggtttttg cttgattcaa aatcacgatt
     2521 tcacttgcaa tcacttcgaa ttcaccagtg gccattttag gatttactgc attttctccg
     2581 cgtcttctaa cttccccagt cacttcgatc acgtattcac tgcgacattt atccgcgatt
     2641 tcccatgctt ctttgttgat ttcaggatta aagacgattt gaacgattcc ttcacgatca
     2701 cgtaggtcga taaagattac tcccccaagg tctcgtcttt tttgtaccca tccttttaat
     2761 gtaactgttt gttctaattg ttgttcggaa actaatccgc aatagatcgt tctttgtgcc
     2821 atttattttt ttcctcctac tcgaataaag tcatttcatc atagacttca tcaaaatgtt
     2881 catatatctc ttctaatgga aaactttttt cttttcggct tgccattgct ttgacattaa
     2941 tcgtttgact agcaagctca tcttcaccga tcaccaatac taatttggca ttcgctttat
     3001 ctgctgtctt aaattgcgct tttgctttac ggttcatgaa gtcgcggtca gctgaaaaac
     3061 caaagtttcg aatcgcttgg accactttca atgcttcgat atttgtttcg tctccgagtg
     3121 cgaccacgta agcatccaac tcattaaatg cagggataac tacttcctct gcctccatag
     3181 tcaagagtaa tcgctcgata cccattgcaa atccgaagcc aggtgttgaa ggaccaccta
     3241 gttcttccac tagattgtca taacgtccgc ctgcgcaaat cgttgcttgt gcccccattt
     3301 ttggtgcgtc actcatgatt tcaaaaatcg tatgtgtgta ataatctagt ccgcgaacca
     3361 tattactgtc gatttcataa ggaacagcca atgcatccag catggaaaca acggtatcaa
     3421 aatgtttttg agcaggctcg cttagataat ctaagataga tggagcttct gccacaaatt
     3481 tttgatctcg tttgtcttta ctgtccaata cgcgtaatgg attttcatga aggcggcgtt
     3541 tagagtcttc actcagctct gattcaaaag gtaaaagata gtcgatcaat gcttgacgat
     3601 aggcttgacg cgtttcttta tcacctaatg aattgatgac tagccggatt tgcgtgatcc
     3661 ccaactgttt gaagaaatca agtgccatca ccatgctctc cacatcagtt gcagggtttt
     3721 ctgatccaaa tgcttctact ccgatttgat ggaattgacg taaccgtcct ttttgagggc
     3781 gttcgtagcg aaacattggt cccatataaa aagttttgaa cggtttcgca tactctgggc
     3841 cgaacaattt atgctcaaca aaagaacgaa cgattggtgc tgtaccttct ggtcgaagcg
     3901 tcacatggcg ttcccctttg tcataaaaat catacatctc ttttgaaacg atatccgttg
     3961 tatcccctac acttcgggat atcacttcat agtgttcaaa aatcggtgta cgcatttcat
     4021 tatactgata atctctgaat aataaacgtg ctgtttcttc gacaaactgc catttttctg
     4081 attcgcctgg caaaatatca tttgttcctt ttggtctttg gaaactcata gttttctctc
     4141 cctttcttag ctatgtaaat aaaaaaatca cccttgttca tcatgaacaa gggtgaatga
     4201 aattcataca cggtaccacc taattttgaa agatttctct ttcctcttac gaataacgct
     4261 cgtacgcggg acggcttttc actcgtccat ctccagagtg tctttaggct aatgaaactt
     4321 tagatcattt tcagcctcga tgatcctctc ttgtaaagtt tcaaaaactt actcgctctt
     4381 tcattgacat tcgatattac ttataaccta ctaaaaacca gtctcaaagt caagagatat
     4441 ctgactattt cccctcacaa aaacgtttta gttcagcttt ttggtatttt gttttagact
     4501 agacaaaaag gaggaatcag ctatggcaga aataagaatc ggacattcac aagtctacgc
     4561 agaacagctt ggattaggag caaatgccgt tggaggacat aatctttttg acggtttgga
     4621 agatgagaca ggaaaacaag tagttcgtac agctttgaat agcgggatca atttgatcga
     4681 taccgcttat gcttacggaa acggccgttc cgaagaactg attggcgagg tactgaagga
     4741 aaaagaatat gatcgctcac gtgtcgtcat cgctacaaaa gctgctcatg taccaaataa
     4801 agggcgaaca tttgataatt ctcctgagtt tctcaaacaa tcggtggaag atgcactaaa
     4861 acgtctgcaa actgattaca tcgacatttt ttatatccac tttccggatg aaagcacacc
     4921 aaaaaatgaa tcagttgcta cattgcacga attaaaagaa gcaggaaaaa tccgagcagt
     4981 cggtgtgtca aacttcacgt tagaacaatt gaaagaagcg aatgcagatg gatatgtcga
     5041 tgtagtagaa gataaataca gtttgatcca tcggcaggct gaaaaagaat tattccctta
     5101 tcttgaaaaa aacaaaatca gttttgttcc ctattttcct cttgcttccg gtttgctgac
     5161 aggaaaatat gaacttggtg aggaaaaaca atttggtgaa ggagatcctc gaaaaagaaa
     5221 tccagatttc caaggagaac gtttcagaga aattttaacc gctgtcgatg tacttcgccc
     5281 cattgctaaa agataccaag ccactcctgc gcaacttgtt ttagcttggt acatgaagaa
     5341 tccacgagtg tctgtagtta tacctggagc taagcgtcca gagcaagtat ctgacaatgt
     5401 ccaagcttta gatttgcatt tatccaatga agattatcaa accattgatg aagcatttcg
     5461 tggattttga tcaattgacc ttatcaattt atagtaatat gaaaaaagga agatcagcac
     5521 tgtttgatct tccttttttt cgataagttt atgctaatct aattcttgta aagtagcgct
     5581 cggctattcg gataagtcgg aaactgattg aatggaatcg tccaatcttg ttcaggtaca
     5641 gagaactcaa acttatttac tagctgatcg gaaaacacac gtagactatg aagagtgatc
     5701 cattctcctg cacagcgatg catttgtcga aaatttcccc cgccttgtgc aatcatctca
     5761 tattcttctt tataggaaat atcttttgct ttccctacat accgtttgat cataaaggaa
     5821 tctggcgctt caactgttcg ttcatcatgg tctgttccat ataaatctaa aataacccaa
     5881 ctgccttctg gtatacgata accatctact tctacttctt tcagcgaaat cgcaggtagc
     5941 attgggaaga aaggataata gcgtctcatt tcttgaataa acggatcttg taacgtcgaa
     6001 aagtcatttt ttaattgctg atacaaatct ggtctactga aaagtgcatg tcccatcaat
     6061 gccgcccaca cagttagagc aaccgttgga cggatgatgt tcaataattc aacagctgcc
     6121 acttccagag gtaaaagctg accatctaaa tctgtggcat tggcaaaagc atataatgcc
     6181 acattttctt ttccagccac aggattttgt cgagcttctt caatcaagcc ttgcgcccat
     6241 ttttctgatt ttttgcgatt ctcgacacct tttatgtgat caatcggaga agtaaacgtt
     6301 ccgctgatca tagagatttg atattctgct aatttttcga cctcatcttt agaaatcgct
     6361 gataaattga tacctgccca ctcgcaaata gatgtaaaga aaaccatctt acttaaatca
     6421 aatagttcaa attgaccatg ctgtgcttca agtgcttgtg tcagattctt atctaagata
     6481 cgatgataat cttccattct ttccggtgtc attagatcca taaaaatatt tttccgatgg
     6541 tgatgagcag ctccatccaa agtctgtact ccaccttgac caaaaagtgt cttcaatact
     6601 aattttggca tagcaccttt tcgtttgaaa ttccttggat cataaaatac ttttgctgct
     6661 gatgatccgt agatcgtgat agcttcttta ttgaaaatct ttgctttgac gacgggagcg
     6721 tccgcttcat ggcgtaactc ctccaacatg ttgtatcctt tttgatacaa tttttttaaa
     6781 tctgtgattt tgatatctac tactggtact tctttcatct tcagcaacct cctataaaaa
     6841 ataacttgta taatttaagc ttaacttttt ttccgactgc tgacaaaaga agcgcttttt
     6901 gcagacaaaa aagccactta agttagagtt tatcagtcta acttaagcga cgtgtttcat
     6961 agtttttact agtgaaaacc agattacttt atttgttttt agaatcaata atgatcgtta
     7021 caggtccatc attgacgaga gaaaccgaca tatctgctcc gaattgtccg gtagcgactg
     7081 tgatgccgcg acttctgatt ccttcattga atgattcgta taaaggaata gctgtttctg
     7141 gtcgtgcggc ttccacaaag cttggtcgat ttcctttttt cgtatccgca aacagtgtga
     7201 attgagagat acttaaaatt tgtcctccca catcttctac actaatattc atttttcctt
     7261 gttcatcttc aaaaatacgc atttgtgcaa ttttttttac cagatagtcc acatcttttt
     7321 gcgtatcggt ttcatgaatg cctaataaaa caagtaggcc gtagccaatt tttccaattt
     7381 cttgctgatc gactgacaca gcagctttac ttacccgttg aacaactgct ctcatatctg
     7441 tcctcctatc cattcgtccg gcgtacactg tagacatcag gtactgcttt gattttatcc
     7501 acgatactct ttaggtggga taaattctgg ataccaaccg tcaaatggat cgtcgccatt
     7561 ttatctttat tcgtccgtgc ttcaacgctg ttgagatttt tcgttagcgc attgactgtt
     7621 tgaagtacat cattcaataa gcctgaacga ttatagccgt aaatttccaa atctgcatca
     7681 tactcttttc gtgtattcga tgtatcttcc cattcgactt caatcaaacg ttctgctaca
     7741 tttggtttgt caggctgaac attcggacaa tctcgacgat gaatggatat ccctctgcct
     7801 ttagtgatat aaccaacgat atcatcacca ggaatcggat tacagcagcg actgatacga
     7861 attagtaagt tttctacccc ttgaatgaca acgccacctt catgacgtac tttcattttg
     7921 tcaggttctt ttttcggctg attcatgatt tcttctgctt cttgcttttg ctgctcgatt
     7981 ttttgttctt tacgttcttt ttcagtcaga cgattggcca tcgtcaaggg gctaacttct
     8041 ccatacccta cagctgcata gagatcatct tctgtttgat aattaaaacg atcgagtgct
     8101 tcttgcagtt tgttcttcgt caaaatatct ttaggcgtaa atcctagatc tgtaatacat
     8161 ttgaccacgg attcgtggcc tttgatcaca ttttcttctc gatcttgggc tttgaagaaa
     8221 cgtttgatct tatttcttgc tttgctagta gcaactaatt tcaaccagtc gcgacttggg
     8281 ccaaatgaat ttggagaagt catgatctca atgatatctc cgtttttcaa tttgtaatca
     8341 agctgcacca ttttgccatt tacttttgca ccagtggttt tgttaccgat atctgtatgg
     8401 atactgtatg caaagtccaa tggtccggat ccttttggta actcagtaac gtctcctttt
     8461 ggtgtgaaga cgtagacttt atcactaaag atatctcctt ttacgccttc cataaattca
     8521 gaagcatcat agctttcgtc ttgaagttcg agtatctcat ggaaccagct taattgtttc
     8581 gtcataccat ctggttctac tttttcattt tttccttctt tataggccca gtgtgcagca
     8641 accccgaatt cagcgatttc atgcatttcc tgtgttcgga tttgaatttc taccggattt
     8701 cctgccggac cgatcacggt agtatgcaaa gattggtaca tattagcttt tggcatggca
     8761 atataatcct taaatctgcc gggcattggt ttccattttg tgtggattgc tcctaataca
     8821 gcataacaat ccttgattga atctacgatc actcgaatag ctagcaagtc ataaatttcg
     8881 ttgaactgct ttttctgatc tttcatttta cgatagattg aataaatgtg ttttggccgt
     8941 ccatagattt ctgcaaaaat ccccagctct tctgtcgcta ttcgaatatc ttcaacagta
     9001 ccacttacgt atttttcacg ttcttctctc ttcgtctgca tcaagtggac gatacggtaa
     9061 tactgttttg gatttagata acgaagtgct gtatcttcta attcccattt gatccggctg
     9121 atccctagac gatgtgcaag aggcgcatag atttctaaag tttcctgagc gatccttctt
     9181 tgcttatctt ctcgcaggtg cttcaacgta cgcatgttat gtagtctatc agctaatttg
     9241 accatgataa ctcgtaaatc ttgagccatt gctagcagca tctttcggtg gttttctgct
     9301 agctgctctt cgtgggattt atattttatt ttccctaatt tggttacgcc gtccactaac
     9361 atagcaacgt catcgccaaa ttcttctctc agatcttcta aagtgatttc tgtatcttca
     9421 acaacatcat gtaagaagcc tgtagccact gtatggggat ccatttttaa atctgctaat
     9481 atacctgcga cttggatagg atgaataatg tagggttcgc ctgattttct aaactgtccg
     9541 tcatgtgcag cagtcgcgta ttcgcatgct ttctcaacaa atgctacatg ttcgggaccc
     9601 atgtattgac taacgagctt tatgacaccg ggtcccgtta aaatttcttc ttttgtcaca
     9661 ggaatcgatc cccctttctt ttttcggtaa aacgggaatc gggacaaata tctgtttgat
     9721 aattgtccca aaatccgtgc agttatacgt ttttttacgg aaatgttcgc tttttaaaag
     9781 ctttttcaaa cgatttttcg tatccattgt ataaataatt gtttctatta tacaaattgt
     9841 tgactatttt ttcaattctt tctgtcgata ctcttttatt aatgaagaac gttagctgaa
     9901 agaaggattc attaattccc attgatagct agcagcactt aataaataaa gaggtgctgt
     9961 ttctgtacga aggattcttg gtcctaatcc acaaagtatc cctttgtgtt ctacaaaata
    10021 atcgacttca tcgcgggata atccaccttc tggtccaaag acagctaaga ttcgactatc
    10081 tggttttgtt tcactaagaa ttttggctgc atttgatttt tcgccttgct ttgctgattc
    10141 ctcataagca accaagacca cgtcataatc atctagccgc tcaaataaat cttgtttcgt
    10201 ttccaataac tctactgatg gcacgatctg ccgatgggat tgttccgctg cctcttgagc
    10261 aatcttttca agccgttgtt gtcgtttcat acgttttttt gcatcccatt taacgacaga
    10321 ggctgatgcc gggaaaccaa gaaaagcttg cgctcctaat tcagtggatt tctgaacgat
    10381 ccaatccagc ttttcccctt taggatagcc actcgctatg gtaatcgaac ttggcagttc
    10441 cttttgattt tcttcttttt taatttcttt cagataaaca gcctcttcag taatctccgt
    10501 gatttctgca acaatggcta tttgatcatt gaatgccaaa aaaacttcat cctcgggttc
    10561 catcctcata acccgtacaa tgtggtgata gttttcccct tctaaaacaa aatagtcttt
    10621 tgattcatat gattctttta aaaaataccg ctgcatctat tccacctctg gttttttcaa
    10681 aataattgca taccagtctt tttgctggaa aatttgatcc acgataaacc cttgttctaa
    10741 catggcatcc agaaccattt gcttcttctc ttcaatgatt cctgaaacga tcagcgtgcc
    10801 tgtttgcttc agtaaacgcc aagcatctgg gatcatcaat aagatgatat ctgctaaaat
    10861 gttcgcaacg attacatcac tttcaatctc gattcctgtc aaaagatcgt tagctgatac
    10921 atggacatct ttggctattt cattcatatc catattttct ttcgccgaac gaacggctac
    10981 ttcgtctaaa tcaaaggcat agacatcttt tgcaccaaga tattttgctg caatacttag
    11041 cacgcccgac ccagttccca catctagcaa tgtttcacca ccacgcaaga ctgtttctag
    11101 tgctgtcaat gtcaaggaag tcgtgggatg agtgcctgtg ccaaatgcca taccaggatc
    11161 tagtgtgata attttttcta gaggatcttc tgtctggtag ttttcccagc ttggcgcaac
    11221 cgtcagcatt cgcgtaatac gcaccggatg atagtatttc ttccaggctg ttgcccagtt
    11281 gctttcctct acttcactaa ctgttacttt attttttcca atagctagac caaactcagg
    11341 taattgtgtg atacgatctt tgataaaagg caagatttcc ggtaagaaag tcgtttccgg
    11401 aaaataagcc atgactagtg cgccttcttt cagcgatgta aaatcatttt tatctaaaat
    11461 ctcaccatat tgatccgact gaaaatgttc aacatctaaa gaatcttcaa tcgcgacacc
    11521 actcgcccct gcctccatca aaatattagc aactgcttcg actgcttcac tttcagtttc
    11581 tacttttact tcattccatt tcatatctgt cactccttaa taacttgggt acggtaaata
    11641 agtatctgct tcattttgct gttttacttc tactaattct gctagttgtt gattatccca
    11701 gtgaagctcg aatacagctt cgtcatcgtc ttcatccaaa aaatcaagca aatcgctttg
    11761 cccttgagct aatacttcat ttagatattc cactaaagaa tcagctactg attttgccaa
    11821 cccttttttt ccttcataag ggattactgc tagataattg cgttcatcaa aaacagattt
    11881 ctgaggatta aaaagaagta cgccgtcttc aaattcgata attggttctt gcgaaacgat
    11941 cccttctgca tcatcaatct caaattgtgc tttattttct gcaaacaagc gaacaacgat
    12001 ctcgatcgta tgattttttg tatcccaatc taaagctaca tcatagtctg tcacttgttt
    12061 ttcaattatt tcatctaaat atgttaatag atccttcttt ttcattttct ttcctccaac
    12121 cccaatcttt ctcgatttgg ttttttcttt gtaaagagat ataaggattt cccgacacaa
    12181 caaaacgcaa cggcttttct gtccatacac ctttgtttgg gataccgatt ctaggcaaag
    12241 cattgatctt ttttggtgtc ttttttttct caaaaactaa gcgtaacgaa ctatctgcta
    12301 tagactgacc ataaagttct tgaggaattg ctaatgcttc cactaatttt cccggaccgt
    12361 tgctgatatc tggaccagtt tttccgccac gatttttact catttgatca atcattgcgg
    12421 cgggttcgat tgcacgtatc attactcctt gaggaatccc ttctggctga gtaatgatgt
    12481 tcaaaatccg atgagtatgc atcgtgtaaa ggtagatcgt acctggtttt tcatacattg
    12541 cccgcacacg cggtgttctt cttaaaccat agctatgggc ggcctcgtct tctggaccta
    12601 ggtatgcttc cgcgtctaca atatatccag ctaatcttcc ttctggtgtg tcatgctcca
    12661 aatacatccc caataaatcc cgtgccactt ctggcgtcgt tttattccaa aaaaattcat
    12721 ctatcttttg tttatccgtc atttttctca cctactttat catacacaat attagttgga
    12781 taagctatga aaacaaaaca acagaaggct gttctttcta ggcatcttgc taaatatgat
    12841 ataattaagg gacgtaaaaa caagaaggag gaaaatccta tgcaacagcc attggcttat
    12901 cgcatgagac ctcgaacgat cgatgaagtc gttggtcagc agcatctagt cggggaagga
    12961 aaaatcatac gccgcatggt cgatgctcgc atgctctctt ccatgattct atatggacct
    13021 cctggtactg gaaaaacgag tatcgcaagt gcgatcgccg gctctactaa ttatgcattt
    13081 cgcatgctaa acgccgctac agattcaaaa aaggatctac aagttgtagc ggaagaagca
    13141 aaaatgagtg gtacagtgat cctcttattg gatgaagtac accgattaga taaaacaaaa
    13201 caggattttc tgcttcctca tttagaaagt ggccgaatca tcttgatcgg agcgactact
    13261 gagaatcctt atattaccat caatccagcg atccgcagcc gtacacagat ctttgaagtc
    13321 aaaccattaa cagaacaaga catccagttg gcagtcgaac atgctttaag ggataaagaa
    13381 cgcggccttg gacaacaagc aatccagtta gacgaagatg ctcttcttca cctttcgcgt
    13441 gcaacgaacg gcgatttgcg gagtgcattg aatgggctgg aactggctac actatcgact
    13501 ccttcagata aagaaggaag gattcatctt acactgtcga tcattgaaga atgtgtccaa
    13561 agaaaagctc ttacccatga caaaaatgga gatgctcatt atgacgttat ttcggctttt
    13621 caaaaatcga ttcgtggaag tgatgtggat gctgcacttc attatctcgc ccgtttagtt
    13681 gaggcaggtg atctggcaag tatttgccgg cgtctgatgg ttatcggtta tgaagatata
    13741 ggtttaggta atcctgctgc tgcggctcgt acagtgaacg ctgtattagc tgctgaaaaa
    13801 ttgggattgc ctgaggcacg tatcccacta gcagacgtcg tcgtcgatct ttgcttgtcg
    13861 ccaaaatcca attctgccta catggcatta gatgcagcac ttgctgatat tcgagagggc
    13921 aaagctggag atgttcctga tcatttgaga gatagccatt acaaaggtgc gaaatcactc
    13981 aaccgtggcg tgggttatca gtatcctcat cattttgatc aggcttgggt caatcagcaa
    14041 tatttaccag ataaactgaa gaatgctcag tattatcagc caaaagatac agggaaatat
    14101 gaacaggctc tgggtcaaca gtattaccgt ataaaagagt ggaaaaagca tcctccaaaa
    14161 aactagacaa gaaaacggtt ctgtttctgc gagctctctc attgctcttt ttaaattcat
    14221 atgctactat agaggtggga aatctgtgat gttcgaattg tcctctttgt gtgttgaccg
    14281 aacattttta ttgatatctt gggacccgtc ttgtgtcact gctggtaaca tgccttatca
    14341 tttggtagtt cgaagctacg accatgggac ccacctgctt gaattcgcgg gatcaatact
    14401 acaggacaaa caacggcatt aacggagatt accatctttt aaagaataaa gcaatttgct
    14461 cttggcaaat tgctttttct atttgttgct tattttggag gatttgttca tgattaggat
    14521 tttaatgatt atcgccactt tagtattatt attcgttagc tattatttat ttaataaaca
    14581 ggatatcttt tttgtattga tcaacaaaaa tgataaaaat caaggattcc tacaatttta
    14641 cggtgctgca tatgctgttt taggcgtaat gggtatttta acagcttttt tcaatcaacg
    14701 ctttattgcc ttggtctttt tactgatcgt aattattgtt tctgccacct tcagtatccg
    14761 atttgcaaaa aaaattgctg aaccaaagca gtaaataatt ctctttttcc ctaaagtgtt
    14821 ctacaataaa agagtacaat acgattgact tgaggagtga atgcttatgt tacaacaata
    14881 caaaaaaatc atggttgccg tagatggttc tgatgaagct gaattagctt ttaaaaaggc
    14941 ggtcaatgta gctatccgaa ataacggaga acttctatta gcacatgtca ttgacacacg
    15001 ttctttccaa accgtttcat cctttgatgg aatgcttgca gaacaagcta ctgaaatggc
    15061 aaagcaaaca ttggcagatt atgaaagcaa tgcgaagaaa gctggcttga ataatgtaac
    15121 ctctgtcgta gaatatggct caccaaaaca aattattgcc agagaaatcc cagaagataa
    15181 tcaagtagat ttgattatgt taggagccac aggattgaat gctgtagaac gtttatttat
    15241 tggttctgtt tctgaatatg ttattagaaa cgcagcttgc gatgtgcttg ttgtccgaac
    15301 tgatttagaa aaccaattgc cagctaaaga ccacaccaaa gcttaacacg tagacgacct
    15361 gccaactatc ggcaggtttt tttgcacaaa aaaccgtgat aaaaacaaaa gacgcggcaa
    15421 gcactcatcg gtctttataa tgtttaaaat aaagggaaaa cagtcaatga gttcaagtaa
    15481 aataaaaaaa gtgaacacca gggcgattag tcgccctggt gtggtttaaa acgacctaag
    15541 catgccaaag cttattggtt cgtcaatgtg gaagtttccg atgaatctga tacgaaaaaa
    15601 tatgaagtat cgatggcgga taccgatttt atcgccaaac taaaatttta tttcgggttc
    15661 gggtaccgcg agaaggcaag tgctacattc ggtaaatcat tcaagaattg atgaaaggct
    15721 accgcttgcc attgcaacca caacgatact cctgaacgcg atgttggtga gatttccaaa
    15781 gagaagagct gtttaatgca acggtattaa gcaaatggca aaaggaagtc atgtagacaa
    15841 agttttttat caaacaccat atgatttcgc ctgaacgctg gtttagctca gaatacaatg
    15901 atgtggtcca cgagacatta ccctcagtca gataaaaaaa gctctctgac agaattacac
    15961 atcgataaat attaagtaaa tgtcagcgct agttgaactt tctgacactt tcaggtacac
    16021 tggaaaagac aactacacga aggagggaaa atactatgat gcgcatgact aaaataacaa
    16081 gtaatgaaaa ttaccaacga taccatatcg catttagcca tgcaattggt tttacagttg
    16141 ctgttgacgg gacaagtttg tccaaaacag ttgaattcac gtaaaatcaa tggccatctc
    16201 aaggtatttt tctcatgata atctgagttt aaataacaca ggccagggat aaccatggcc
    16261 tttttgagtg gtctcaagga gagagatcat gatcatacaa gcaatttcta ttaaaaaaac
    16321 aatcggcggg aatccattat ttgaggattt atcctgtcaa attgaagctg gtgaaaaaat
    16381 tgccttagtc gatatcaacg gaactggcaa atcaaccttt ttacaaatct tgaccggcag
    16441 agaaggtgtc gacagcggtg ttatcagtcg caaaaaaggc ttaaagatcg ggacggtcga
    16501 acaagaactt acagtttccg aagcgaccgt caaccactac ttgctccatt ctgccgctga
    16561 gatacaggac ctcaaacagc aaatgagtcg ctacgaagca ttgatgacag aaccagatac
    16621 aaacttagag aagtgtctgg cccgttacgg tgagctgcag catcgctttg aagaactagg
    16681 cggttacgta ttagaagacc ggattgcagc gatcttgcaa ggcttaggga ttccacactg
    16741 tcaggaagca cggttaacag aacttagcgg aggcgaacga gttaaagtcg ctttagccaa
    16801 aattttagca gccgatgccg atttactttt actcgatgaa ccgaccaatc atttagatct
    16861 taacagtatt cgctggttgg aaaactattt acagaataca aaaaaatcgg ttctggtcgt
    16921 ttctcatgat cggcaatttc tcgatcaagt caccacaaaa acatgggaac tggaagaagg
    16981 tgcattgatt gagtatcccg gaaactacag ccgttttcga gtcttgaaag aagctcgctt
    17041 ggctgagctg acaaaaaatt acgaactgca gcaaaaagaa gtccagcggt tgaaagtcat
    17101 gattcgtcgt tttcgtcagt gggcacatga aggagacaat gagtccttct tcaaaaaagc
    17161 caaagaactg gaacgccgcc tggcgaaact gacactcgtc aaaccgccac ctcccccgaa
    17221 aaatcggctg caaagcttgt ctaatggtgg aaaatctggc aaagaagttt tcatcattca
    17281 aaacttgcac cagcagtacg ctgatcaagt actattcaaa gacagcagtt ttgcagtcta
    17341 tcgtggagat catttagcca tcatcggcga caatggtgca ggaaaatcaa ccttactcaa
    17401 attattactc ggtgaggaaa agccttacgg tggcaccatc aaattaggta gtagcttgca
    17461 aatcggttat ttgccgcagc agctgcagtt tgccgatccc gatagccgtt tgctggctta
    17521 cgccatcaca ttgactggaa acgaagaagt cgctcgacgg caattagcca aaagtggctt
    17581 ttatcaaagc gatgtgggta aacggatcaa agatttatcc ggtggtgaaa aaatccgttt
    17641 ggcgttgctg aaactctttt tagaaaaaat caacgtcctg attttggatg aaccaaccaa
    17701 tcacctcgac agctatgctc gagaagaaat tgaagaaatg ctgcaagact atcaaggaac
    17761 gctcctagca gtcagccatg accgctactt tttgcagcaa cacttccaag aagcactcgt
    17821 tattgatcag gagcagatca gccgccagcc gctcggccaa ctgattgact aggacgtttt
    17881 ttcgcaacaa aaaacttgat tttcatacct gtgctttcac gattcaggca aagtacaggt
    17941 ggaaaatcaa gtttttatta ttcgatcaat gaatgtttcg gatcggtttt ctagctaaac
    18001 ataaagcgaa gaataaagtc gacagcccac gaggctgatc taaaaatgtt ttttcttttt
    18061 acatatgtaa tgtcccttat aaagatgaat gaaaataaaa tattgggatt ttcctgaaaa
    18121 tcaataaata aaatgccagt cttttccctt ttgaccactt ttccaagcaa aattccctaa
    18181 gaagatcctg taaaaaagaa gggaaattga agttaaatta ccattttgcg gtcttctccg
    18241 caaaaaaaac acatgcaaga attccctctt gcatgcgttt catcttttat cctaacgaat
    18301 gctttggctt agccgcgcag acgctccaca tcacgggcaa tcattagttc ctcatctgtt
    18361 gggatcacta atactcttac ttttgattct tttgtagaga tatctacttc ttcaccacgc
    18421 aattcattca acgctggatc gatttcacaa ccaaaccaag acataccttt gatgatttca
    18481 ctacgtacat gtccgtcatt ctcgccgata ccagcagtaa agacaattgc atcaacacca
    18541 ttcatcgttg tcacataacc gccgatgtat tttcggatgc ggtccacgaa aatgtcataa
    18601 gctaaacgaa tatgttcttt ttcatagttc ttttcaagat cacgcatatc actagacaat
    18661 cctgttaggc ctagcagacc agatttcttg ttcaaaacat tgatcatttc atcaatatca
    18721 atctctaatt tttccattaa atatgggaga acagctggat cgatatcacc tgaacgtgta
    18781 cccattgtca ccccagctaa tggtgtgaat cccatagaag tatccatgga tttacctcca
    18841 tcgactgctg tgatcgatac accatttcct aagtggcaag tgatgatttt tagttcttcg
    18901 atcggtttgt tcaataattt tgccgcacgt tccgacacat aacgatgact tgtgccatgg
    18961 aagccatatt ttcttacttt gaaatcttca taataagaca ccggtaagct atataaataa
    19021 ttttgctttg gcattgttga gtggaaagat gtatcgaata cagccacact caaaatgtca
    19081 ggtaaaatct ttttaaacgc tttgatcccc attaagtttg caggattatg caaaggagca
    19141 aattctgcta attcagcaat gcggtccatt actttctcat cgatcactac tgaatcccca
    19201 taagtttctc ctccgtgaac aacacggtgc cctactccgg tgatttcatc ataagagctt
    19261 aaaatttgca gttcaatcaa ctgatctaac agcattttta ctgctacttc gtggttttcg
    19321 atatccatga tttgttcgaa tttttgatta tcgccgtact tgatggtaaa aatagaatct
    19381 tgtaaaccga tacgttcgac gatccctttc gcaactacgg tttcttctgg catttgatat
    19441 aattgccatt tcaaacttga actcccagca ttgattgcaa ttgttttaga catatgactc
    19501 tccttctcgt tgaatgatta cagagcagat gatttccagt ttttaaactc gttgaaaaat
    19561 tccgtcacct tctctggttc ttttaatgaa gcaagtttca caagcaagac ttcttttact
    19621 tgttgtgctt gtggtccttt tttctgtaag atcaaaatac ttttttgcga ttgtttatta
    19681 cggaacaatt catctggcaa ttgaatcatt ccttgaagat aaccttcttc tttaaaccat
    19741 tttttgattt cttcactttg atccgtttct aaaaatccac tcggcatcaa aaacaaacca
    19801 aaaccatccg gcttcacata tttcattgat tgctctagca ataaatgatg tgcataacta
    19861 tggccttctt cggtacttgt taaaaactct tttgcttttt cgtcattcgg ataataaccg
    19921 attggcaaat cgctgattgc aaaatcgaca ggatctatta ataattcctg caacccgtct
    19981 tgatgaaagt attgaacatt tgcttgtgtc aaatcacttg tagaagctgc gacagccaac
    20041 aatgtatcat caatatccac accaattcct tctgtttggt accccgcatt cgataggttt
    20101 aaaagaactg ttaataacaa gttgcccatt cctgccgcaa tatccagaat cttaaccggt
    20161 gcttttttat ttgtgtatag ttgttcaata agaaaaacaa ataaaaaacc gatactatca
    20221 ggagtgagtt gatggttcgc ctgcaaatgt tctgtctggc tgcccttcaa taaaagtaat
    20281 tgagacagtc tgcgccattc ttctggctcg aaagaaagtt tcttcaactc ttcataaaga
    20341 gctgtgatcc gttgcgttgt ctcttttgtc ggcactccat ccaccacacg tacttgataa
    20401 tcatctatta aattttctgc attttcgaca taggcttcta aaaaggaagt tcccaatgca
    20461 ttctgtaaaa gctgaatagc ttccaggttt tgattaaacc cttgttccat tttttctggt
    20521 aacatggtgc acctcatttc tagatatatt ataacaattt tttcacctgt ttaattctat
    20581 cgaacaattg acaaaatttc aatcgttttc actgtttttt atttgtgaaa cggcaaacat
    20641 ttttttatta aaaacagtgt tataacagaa aagagggcgt tttcacgaac attccatcat
    20701 tctgtactag tatctgtatc attttttact tcttctgtga accgaaagat aaacggtgag
    20761 acttccacct ctaagaatag ggactgttgt acccactgat atgttacaga tccagttgtg
    20821 tagtaaaaaa ctccttccga actttcggca agcgtagact gttccgataa agccatctgc
    20881 ttcattattt ttgctatata ataatttttg attcgtatat taagatccat tgacaaacgg
    20941 tagctttcta atgctgccat caataaaaac gtacataaaa gaaataaaag caagacagaa
    21001 aataaaatag agcccttata acattttcta ctgatcgttt tttgtccata tggcatatcc
    21061 atcttctcca ttttcaaagt gtacataaaa agaaattgtg tgttcttttt ccaataattg
    21121 aagctcagtg atacttgtta acataggttg atgtccgccg gaagttcttc ttttacgtat
    21181 cattgcctga tatctttcga tgatatactg tttatcctcg atcgtgtagt gcagttgctg
    21241 atttttcaca gagacattgt gactgctctt gagttcttct tctaattgtg ctaaaaatat
    21301 tagccactct ttttgatctt tacgctgcaa aaattcactt acttggatgg tttgctgaat
    21361 caaaagattg aatgtgagca atagactgct caatatcatc agtccaatta gacattcaat
    21421 cagcgtaaat gctggaatta cttgtttttt caatactgat cgcttctttc tccatattga
    21481 caaacacttt ttcgattact ccattattat cgacaatttg gatatcgtat gggatcattt
    21541 cttcccttcg ctctggtctt ttgtgataca tccgctgcgt cttgatatct tcataaatca
    21601 ttcgaataaa tagcagttcc tccccacttt tcttttctgc ctgaagcaaa aacagctggc
    21661 caaagctcac taagtagaca cagacagaag cgatcataag cgctgtaagg gactccaaca
    21721 aaacaaatcc tgttttattc aattcttttt gtatagcgac cactccctat ctggaattga
    21781 tacgtaatcg tccgctcttt ttcttcccaa taaaattgat aagcttttag tgaactttca
    21841 ttcccggttc tagcagcaaa agtaatagaa gcatggcgtt tcaaggtaat ctcatcagga
    21901 atatctaata cttcccaatt tattttatct ggattcggaa catgaaaaat gatttgattc
    21961 tcttcactat tccaataaaa gcctgtttgg atctgattca caatagcgat tttttgcgta
    22021 gaatagattc gtttttcaaa ttggccgaaa aattgataga ctgccaattc attcttccat
    22081 gcagaaaatg cgagaactgg aaaagaaagg atcgttgtaa gtaataaaat cagtaagagt
    22141 gattcaaaca acgtatatcc tgatgctaaa accttacctt caaggccgct gatagatttc
    22201 tgcatgttct cgtttgatat agccttcatt caacaattcc tccacagtag gaactcttcc
    22261 attattttga agcgcataga gctctttttg tgtgtctacc aactggatga tcgcttcacg
    22321 actttcttgg tctacatgat tcctataacg cgacaaatta gggacgaaca acaacactaa
    22381 tacactgatg atcagtaaga cgattagcat ttcgatcaat gtaaaaccgc tataataaga
    22441 tatttttctc tttttcatgg caaaacctcc tctatgcttc cgtaaacagg taataaaaca
    22501 gcggcataaa ctaataaaat caaagccgct acgcccaaaa aaatgattgg ctgaataaaa
    22561 tgcatccagt gatgaagacg tttgaaaagc tgttttcttg tccattcgct gtaaaaaatc
    22621 agttctttac ctaaacttcc ctttgcctct ccctgtaaaa caattttact gaattcttct
    22681 gatagaaaac gataattctg gaactgctca gccaatgaca tacctgcctc caatccttga
    22741 atcaacttag aggcaagatg ctgaatcaga ctttcttgtt tcgtttcctt taagcagact
    22801 atgatttgcc ttaattcgaa accttcatga aaaagctttc caatctctag tgacaaataa
    22861 gccgattgat acaaggaata tatcggtcca atcaataatt gtctgcttat ccattcactc
    22921 tgatcaacag gagacaactt catcagacga taccttacta gtaaagccaa tagagtaacc
    22981 gctaggatga atagtagaac ataccaatga aagacttgta tcagctgaat accccaatgc
    23041 tcttgcatga ccattcctga accaataagc tgaggcaaaa cgaacaaccg catactaaac
    23101 aagataccta gcaaaaaggt gagcaatagc atcggatatg caagcagctt tttcaaatca
    23161 ttgcgaaatc cttcaacaag ccgaaactgt ttggcaattt ccaacaaagt tgtcggcaga
    23221 ttcccatgac gttcagctaa ctcgatctgt accagttgct ctgctccata tcctatttgc
    23281 gaaagtacct cactaaacgc ttgcccttcc tttagctgat gttggatatt tgccaaaata
    23341 acaggtggaa acgaacggat cgtgagtaat aaataaaggg attcttgcag actaaaacca
    23401 gataaaagca gttctcccat ggtttgtgca aactggtttt gctggcttct tgttaatcct
    23461 ttagaatagt tctgactgcc aaatattttc ctgagcttct tcataacttt cctcccatga
    23521 tttttttact gattcttcct ccataaattg ataactccat aaaacagaag gatggtgatt
    23581 ggcatcttcc aaaagttctt gataaatgac accagctaaa cattcagata gctcttcttt
    23641 tttctttgtc agttcatgga ttctgaccaa tgtaccttca agattgcgtg catgtacggt
    23701 tgcaaaaacc cggtgccctg ttagtgctgc tcttaccgca gcatgtgcag ttgattcatc
    23761 tcttatctcg ccaatgatta gcaagtctgg acgatgccgt aatgcgagtt tcaaaagtgt
    23821 atcataagtc tgtgctattt tcggattgat ctgtagctgc agaaaaaacg gttcttctat
    23881 ctctacagga tcttcgatag taatcacttg caatttttct tccaaagcca accgatacat
    23941 tagagaagtc ttgcctgatc cgactgtccc gctgaaaagg tataatccac gtctgacggt
    24001 tttctgtaaa atagttgcaa aatctcgaga actaaagcaa cgatggggtg tttcaaaagt
    24061 accgtataat agccgaatca ctaaactctc tcgttgaagg taatctccta cagaagacaa
    24121 ccgcagacga acttcctgtt tttctaaaga ataagtaaat gcacctaact gagcttttct
    24181 tttttctccg atatccatct gtcccaaata cttaaatctt gagatcagtt gctgtccatc
    24241 tgccgaagag actttcttcc agaatgttcg tttttttcca tagcgaaaat agatgtaaaa
    24301 tacttctgaa gctggcaata tatatacgtc ttgggcatgt ttggcaaaac tttcacttat
    24361 catttgatcc gataatcgtt ttatttccat tattctcccc tccacaaaaa acatacgtct
    24421 ttttgatctt tttgtttcaa ataaaatgaa tttacaaaaa aatattggca aaagccgaat
    24481 ttatgttata cttataaaaa tttatcttgg gaggcactcc attggaaaca acattaagga
    24541 aatatatcga agtagcaagt aaaaaaaagc ctgctgacct agtgatcaag catgctaaga
    24601 tcgtcaacgt ctttacgaaa gaaatcatgg aaaaagatgt agcaatctgc gaaggaatga
    24661 tcgtaggaat cggtgaatac gaaggaaaaa gcgtacataa tgctaatggg caatatctag
    24721 tgccaggatt catcgatggc catgtgcata tcgaaagctc acttttatca ccaagcgaat
    24781 tttcaaaagt atccttgctt catggagtta ctactgtagt gacagatccc catgaaatag
    24841 gaaacattgc tgggagtaca ggtcttgagt ttatgataga agatgcaaga caaacactca
    24901 tgaatatctt ttttatgcta ccttcttgtg tacctgtaac cccttttgag accaatggtg
    24961 caaatttaaa tgcaaaaaaa ttagcccctt ttttacaaaa acccgaagtt ttaggcttag
    25021 ctgaagtcat gaactatcaa gcagtagcaa ataatgaaac agacatccta gaaaagatcc
    25081 aattgatgca ccagcataag aaaaaaattg acgggcatgc agctggtatc ggcatggaag
    25141 agttaaatgt ttatcctgca gcaggtattc ggacggatca tgaagctaca actgcaaaag
    25201 aagctaaaga acgtttggat cttggtatgt atttaatggt tcgagaaggg accgttgcaa
    25261 aagatctggc atcgctacta ccggcgatca cacctgaaaa ttcaagaaga tgcttctttg
    25321 tcaccgatga taaactgatc aatgatttag taaaagaagg atcaatcgat catatcgtcc
    25381 gtcaagctat tgcacttgga atcgatccat tacaagccat tcagatggca acattgaatg
    25441 cagcagaatg ttttgatttg aaaacactag gtgcgattgc ccctgggtat caagctgatt
    25501 tcttcttaac agacgatctt accaagctgc caattcatga agttttctat aagggaacat
    25561 tagttgttca atctgggcaa ctccaagacc atttatttgc tgatcattca aattcatata
    25621 cgtgttgtct gccaaaacta aacgctcaac cattaaatcc acattcgtta gagctgcttc
    25681 tttcttcttc acaagcccat gtgatcgaaa ttatcccgaa tagtctagtg actaaagatt
    25741 tggtagaaaa agtggaccga caaggcgaac gattcattcc ttcaatcgaa aaagaccaat
    25801 taaagattgc ggtgatcgag cgtcaccatc aaacgggaaa cattggatta ggcatagtga
    25861 aaggatttca gctgaaacaa ggagcgatag cgacaacgat tgcccatgac tcgcataatc
    25921 tagttgtcgt tgggacaaac gacgaggata tgctgtatgc tgctaatctt ttgattcaaa
    25981 aaggtggcgg gatgaccgtt gtcaacaaac agcaggaaat agcttgtgtt tctcttccta
    26041 taggcggaat catgacacag gaaccttttt cgatcgtcta tgaacagttg aatgaattaa
    26101 taactaaagc acatcaacta ggagcagcta aaacctttga tccttttttg acactttctt
    26161 tcttaacatt atctgtcata cccgaattga agatcaccga caaaggatta ttttcatttt
    26221 ctcgcttcca attgattcca ccttgtggaa aaaccgatga aataaataca ttttaaatca
    26281 aataaatccc agaaaataca ttacatctgt aatgttcatt ttctgagatt tatttatgga
    26341 tattaatgtt tttatgaaag cagaaagtta gtttcacaga cttttctttt tggtcttatc
    26401 ttgaaattga gcttctactc gataaatacg ttgacctttt gaagaaaatt tttcttcata
    26461 ctccgtcata atattgcctt caaattgact ttgatgtaaa tctaaccata cctgtttgag
    26521 gatcatgcca tattgagaaa aacttgccag agaatactca aacaatcctt gattatctgt
    26581 tttgaaatgg atttctccat ttggacgaag aatctgttca tctacagcaa gaaaagtctt
    26641 atatgtcaat cgccgttttt catgacgttt ttttggccat ggatcggaaa aattcagata
    26701 gatttgatct acttcgctgt cagcgaaata ctctgtcaac gctgatccat ccacatgtag
    26761 aagtttcagg ttaggaagcg gttgttcgat caacttgtct agtgcaatcg aaacaacact
    26821 gacctgcatt tctactccaa tataattgat ttcaggatgg gcttttgcca ttccagtgat
    26881 gaactggcct ttccccatac caatctcaat gtggattgga tgatcgtttt caaacaattc
    26941 attccatttt cccttccaca atgttggatc actaattaca aaattcgggt gagccgctaa
    27001 catttccgct gctcccggac gatttcggac acgcattatt tatctccatt cctttcctct
    27061 aattaaaaag gtctggccaa atctccgcag agtttgggcc aaacctcagc cactgaaaat
    27121 tcgttttaat tgtaaaattt cttcgttcat ccgtcgatga tcttcacgtt tgcaatatct
    27181 ggtgatttct tggagcaaat tcatggccgc ataccaatga aatttttcaa gcatttcgtc
    27241 agtcgggcgt atgccataac tgatcagcca ctgattccag ctagacagtg gcacataatt
    27301 tcctaaaatc gttgcaatat ccaccgcagg gtctgcaaac atgattgaat cccaatcgac
    27361 tagatataaa taatttttac atactaacca attacgatgg tttacatctc cgtgtactac
    27421 tgataagttt tccttatcta ttgacgggat ctgctcacga agaaaacgat ggacaatttg
    27481 cagaaattga tttttctgga gttcttccgg taattctttt tcatattttt gcagcatctt
    27541 ctccggcgtc atgacacgac caccgatacg ggtcagcatg gttttaagag aatttgaatg
    27601 atgtaaatgg tacaaaacat caatcacgtc gtttctttga ccaatctcat taggagtcag
    27661 caagcgacct tccagccact cttgtgctgt caaagtatcg ccattccccg ttcgttttgt
    27721 ccaaacaagt tttggtgtaa ttccttccct tgaaagagca gccaacatcg gagtcgtgtt
    27781 tcgtttaata aagactttgt cctcatcacg catgcctatg tatgtttttc ctgtgtcgcc
    27841 tttgatggga cgtaaccgcc actcttggtc taactgaaac tccatcatgc attctcccgt
    27901 cctcttactc tttgttttgc agtacttatt attctagtcg cctgaacact acacgtcaag
    27961 cacatatacg ttctttttca tttaattgcc attcaaaaaa ccagcttttc tcaaaaaaaa
    28021 tagaacgaac agcattctta ctgtcaacgc tctatttttc attctcattt ttaccctaca
    28081 cagctttgtt ttcagtctga catcttcttc aaacgagttg gtacatatag tttaacaaag
    28141 agcatagtaa caatcaaata agccgctagc ggaacccatc gctgctgccc atctaatacg
    28201 actgcagcaa tcaaaccaaa aataaccgac gcgataatta atacccatgt gagtagatac
    28261 tgaatcgcct gcttcttctg attcttatgg attggataaa gctgaacgag aaccatatag
    28321 cggaactggt tcgctaaagg taacagttgg aaaccgatca aatataaaaa taagaaaccg
    28381 ataatcagac taaaataaaa gtcgactact ccggcaatca ataacccacc tataattact
    28441 aatcggaaat aaagaccact aaaatcagaa cctcttgcca atcttcttgc aaacaagtaa
    28501 agataagtgt tttttgattc tcttttaata tgagtcaata taggatccaa atagcgtctt
    28561 cttttgatct ttgccgtaat ttcaggaaca tctgtgaata aattgataaa ttgatagatc
    28621 tgatgcaacc tatgttcttc tacatgcacc attttctccc aatccaatcc tttttcttgt
    28681 tgcttccaca aaaaccagta atatagactt gcctgtacta atgcacctgc tattcctgcc
    28741 catattagcc aataaaatga tagaaacaaa aagatcagag aagaaaaaaa ccagcctaat
    28801 ttccaataac gaataacttg ttgatctagt cgaaacaatt ctgctctctt gatttgcaga
    28861 tgactgaatt tcaaactcca taatatgatc agatagacga aaaacatcga aaaggattgc
    28921 tccgtactaa ccaccactaa tggcatggcc aatccgcatg ccaatatcaa aaagatcaac
    28981 ggaaaaatgc aagaatagcg aaaagctcgt tccaaatagg tttgcatttc tttttcttta
    29041 ggaagtaaaa aaacaatatc tgctgcctct gcaaaagtag ccatcttacc cacgaataaa
    29101 cagagcatcc aaaatacaga gattatgagc cctccaattg caaacggtgg ctgcagggtt
    29161 ttcaaccaat tagaataata taatcccaca ccgccaatca agaaaagaca aactaaaaca
    29221 aaatgatcgt tcaatacata acgcatatat ttcatcattt tcttttgatg tcttgctaaa
    29281 cgtttttgaa aaaaactact catgtgcctg ctcctttgtt aaagcaatat agatttcgtc
    29341 caaagaagaa tccggcagct gaaattcttt tctcaacatc tcaagagaac cgtctgctcg
    29401 tatctctccg ttatgtaaga ccacaaaacg atcacaatat ttttctgcag tagctaaaat
    29461 atgcgtagac atcaaaatac cggctccttc ttttttcatt tctaccatca attcaagcaa
    29521 tgcattgata gctagtggat ccaagcctaa aaatggttcg tcgatgatat ataaagaagg
    29581 ctcgatcaaa aaggcgcata agatcatcac tttctgtttc attccctttg aaaaattagc
    29641 tgggaaccag tcaagacgat tagataaccg aaaagtctcc aacaactttt ctgctctttt
    29701 cattgctata tccataggta tatcgtaggc catcgcagtg acttcgatat gttctctcaa
    29761 tgtcaattct tcatataaag aaggagtctc tggaatatac ccaatttgtt tgcgatattc
    29821 ttccgtattt ttctttaagg atagatgatt gatttctatc gttccttttg aagcttccag
    29881 cagaccgata atgtgcttga tggtcgtact tttccctgct ccgttcagtc caatcaaccc
    29941 tactatctct ccaggctgta catcgaaact gatatctttt aacacaggga gatgtccgta
    30001 cccccctgtg acatgatcga taattaaact cattcttttt cctccgccat ttatttttat
    30061 ttattatacc acgaagaaaa tgacggtatt ttgttttttt ccttacaata gctttaactt
    30121 atggtaaagt taagctgaag tatttattct ttatagaaag gatgttttat atggaaaact
    30181 gtattttttg caaaatcatc aatcaagaaa tcccaagcta taaaatatat gaagacgaca
    30241 aggtctatgc ttttttagac atctctcaag caacgaaagg acacacatta gttgtgccta
    30301 aacgtcatgt agcagatatt tttgaatatg atcccgaact tgcaggagag gttttttcgc
    30361 gtgtgcctaa gattgctcaa gcattggaaa aagcttttcc agaaatgaaa ggtcttaata
    30421 tcttgaataa caaccgtgaa ctagcctatc aatctgtttt tcattcacat atccatttag
    30481 tccctcgcta cagcaaagag gatgattttt ctattcactt tgtcaatcat caagatagtt
    30541 atggatcgga agaactaaaa gctattcaag aaacaatcgt aaagcaggtg tcctgcgatg
    30601 attaaaaact ttttcaaagg actgttattt ggagctgctg ctggcactgt cggaggtctg
    30661 ttattggcgc ccaggagcgg gaatgaaaca agaaagaaac tgatcgaaga attggaagaa
    30721 gcaacaactc tgacaaatga tttaaataac agcctgaatc attttaaaag cgcattgatc
    30781 gaaacaaaag aaacagctga atctgtgatt cctgctttcc aagaagcaat caaaaaagat
    30841 atcgaagatt ttaaattcca agccgaacca cgtatcacac aaatcaatga acaagtagaa
    30901 aaactaaaag ctgatctgcc agagtatcct gaacaaatat aataatgatt attttctcaa
    30961 cagatttaac tatataagtg agaaaagttt gcgtatttcc taggaatgtg atgaaatttt
    31021 gaattttgtt tgaaactacc acgacaacgt ttttaaagga aatgggatat ggtatagtta
    31081 atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa aaatcaatta
    31141 ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca ggcgatacta
    31201 ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc tatgatgaag
    31261 caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat aaagtattca
    31321 ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac gacaaacaag
    31381 cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat acgaaagata
    31441 cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa gctcacgtag
    31501 atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa gtagaagcac
    31561 aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca gcggatgatg
    31621 gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca aaagaagatg
    31681 gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta aaagaagcag
    31741 ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat cctacttctt
    31801 atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc aatgacatgg
    31861 ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt gacaatgcat
    31921 tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc aaagatgatg
    31981 cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa gactcaagtg
    32041 aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca aaagaatctt
    32101 caactgaaga aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg aagacttatg
    32161 tctcgctttt tttgttttga atgaaaaaaa taaatttttg ctataaatct tttggtacat
    32221 agaaacttcg gttgtccata ccgaaaatcc gatcggtgta ttttcctggt tctgtttgac
    32281 gaatcgaagt caacatcatc tgcatcgaag catcgatatt gtcgatttga tgtaatattt
    32341 ctgcttccat tattcttgga cgtacaggtg agccatattc taataagcca tggtgtgcta
    32401 aaaccatatg acgtaataca agaacatctt cgtcagcttc gctgattttc aacgcaagac
    32461 aggctttagt tatttcttcg tcaatcaata ccagatgacc gatcaaattt cctgcaagcg
    32521 tatattctgt cgacatagga ccggaaagtt caatcacctt tcctaaatca tgcaaaatga
    32581 taccagcata caacaatgct cgattcagct caggatattc atcggcgatt gatttcccta
    32641 agtgaagcat tgtcaaagta tgataagcta atccactggc aaatgcatga tggtttcttt
    32701 ttgctgccgg aaactcaaaa aattgttttt gatatttatt aagtagatag cgaacaatac
    32761 gattccaatg cgcattggtg atctcaaaca acaattgatt gatttcttct tccatttctt
    32821 ctttcttcaa aggggctctt tccatgtata aactaggctc tcctggctca tctacccttg
    32881 ttaagcgcat atgaagaatc tttacttgag ggtttccttg ataaacctct cttttaccgt
    32941 ttagaaaaac gactgtgccg gctgtaaatt tattgatttc ctcctccgaa gcatcccagt
    33001 atttaccatc gatagtacct gaagtatctt ggaaagtaaa agcaataaat ttttttccgt
    33061 ttttagcgat tcttacatct gcacttttga tcaggacaaa ttgttcaaac tgctcatcga
    33121 ttactaaatc acgaattttt ttcattattt gctccttcta attgttttac gatttggttc
    33181 atctcatggt aatagcttac catttcttta tcagaagaaa aacaaattat ttgatacttt
    33241 tctccaaatt tagctaaaag ttcagctaat tgatactttc gctgatgatc ataatgaagc
    33301 cagccatcat caatgatgat cgggcatatc gaacggttag cttctaatga gaggtaagca
    33361 aatctcatcg ccataatcag ctgatctttc gtacctgtag ataactcata gatgggaaat
    33421 tctgtatatc cgtctgttaa agtcagtgta tcatttttca gtaatatctt ctgataattt
    33481 ccttttgtta aaatacctaa ataatcacct gctgctctta acaactgggg taattgtctt
    33541 tcactcatct ctgttgaaag atcacctaag aatgtcccgg cgatttcaag tgctccccat
    33601 tcctccagta attcctgtaa ttttgctttt tgccggctag cttgttgata aagttcgtcc
    33661 aatgtgccgt ctctttgcaa ctcttctact tgaagttgga gtcgttgccg attttcactt
    33721 gcttcttttt cttcttgctg gagttgggat tgcttttctt tcagttggcg cagtcttaca
    33781 gataatttct cttttgtcag ctcttcagga aatagtggag taagcatttc cgataattca
    33841 ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac aggtacttcc
    33901 gaaggataag ataagcctgc ttgagtcaat tgttcgctgt attcttgtat ccatactttt
    33961 tgtttctttt tcaattgatt gatttcttgc ttcaataaag tattttgctg atagcttctg
    34021 gcaaatttca tctgttccat ctcttgtata aaacgggaaa gcaataccat tttttctgaa
    34081 attgtcttat cttgtatcgg taaccattct ttaacaaact caaatttttg gtcgatttgt
    34141 cgtagttttt cttgtatttc ctgttctctt ttatgtttcc gctcaaatac gccatataat
    34201 ttctgataat ctatgacttc ctgtccatat tctttcatga gtttgagcgt atccatcttt
    34261 cctaagtgcc gatgatccgc aaaacgatat acttcttgct gaagaatctg tttttcttct
    34321 ccagttctct tttgtttttc ttctgcttca gctaattgag ctaaggctag atcaagttgt
    34381 cctagttttt cttgccacat tccttttatc tcagagaaat catttttagt tttaattttt
    34441 ggttgccttt tgtagaagaa atagccaatc atgcctgcac caattagtaa aaggatcact
    34501 ttcaagggca ttggtacaaa tatacctgca atgattattg ctccgccagc catcagcata
    34561 tacggtttag atgaagagac tggtgctatc tctgactgat tttgtaataa tgctggatag
    34621 gtctcttcca agtcattcac ttctgcttca atcatctctt gctgttcttt agctatttgg
    34681 agacgcacag aaatctggct ttctagatca gcaatccttc tttcttcagc cagaagatgc
    34741 ttccattctt cttcatataa aaattcttcc ggtaacggat ttttctgcca atgccacctt
    34801 tgttccaaaa tcttcaattc tgaatttgtt tctctagtct caatttgtaa tctttgccat
    34861 tcttcgttta attgttccgc tgtaaattga agttgctgga gatcttttag ttcctgctcc
    34921 tgctctaaat aaaaataata tctggctgac tgctgatcca tcccgctatg cttttccagt
    34981 gttgcttcta attcttccaa acgctcattt gtttgctgat accgtttgga gaaagactcc
    35041 agatctaaaa gaagttgctc atcaggaaga cctgtagtgc tgtttttttt gagagattgc
    35101 cattcttcat ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa ttgtttgttc
    35161 gcctctttta attgtgccaa taactgctgc tgttgcgact cgtattcctg ttcttgttgg
    35221 atcaacttgc caaaataagc ttcctgttct tgtttttgat gaattttttc ttgcagctct
    35281 ttccattcat ttaatttttg attgattagt aatttttggc cttttttctt gaataattgt
    35341 tgtgctttct gataatattc ttgccgcttt tggaatactt gagaactgcc cgtgatgcct
    35401 aaagaaatca gcgcatcatg tagctctttt tctctcaaat gatctagttc catcaactgc
    35461 tcttgctgga aagtaaatac ctgctggaat aactctttcg tcaaaggtgc gatcagtttt
    35521 tctagtaact catcacttcc cacttgatcc cccagccaca ccttcgattt tccgcgattt
    35581 tgctgcttgt agcgctcgac agcaaattca ccatatacag gatgtttaag ccatattttg
    35641 ccgccatagg ctgagccgtc ttttggtgta taatcctttt ttttacggcc ttttgaaggg
    35701 aaaccaaata atattgcttg aataaattga taaatcgttg attttcctgc ttcgtttccc
    35761 ccatagatca gctgattgcc tgaataaaag tcaaaacttt tttgacgcca ttttccaaaa
    35821 gcagcaatct cgatccttaa aatcttcatt cctgatcctc ctcaaataaa aaatcttgat
    35881 taagcagctg cttggctttt tcaagtgttt cttcttgata ttccggcagt tcgctcaata
    35941 ttctagccgc ttcttgatga ctatatactt catttaaaat ttgttggaaa tcttgaggtg
    36001 tctgatagta ttgaaataac tgttccatca atttagcaga agcagcaagt ggggtcttcg
    36061 tataaaaagt attctctatg agagaaatac gccaaataaa gaaatcattt atcctatctg
    36121 agaattcctc taacaaatag tctaatagct ctcctgattg tatccgttcc aaaacatctg
    36181 tattgagatg gtcataatct tttaatttta tctcaattaa actaaatcca tcctctactt
    36241 ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt cgttcttgct ttcctcaatg
    36301 atatttcctt tgtcttccaa tagacctctg ctactttaat cggatgtatt tggcaacttc
    36361 ctttacttaa ttcaactagt agaatagatg tagaagcttg ttccttttta gtatgacctt
    36421 gaggcgcacc tggataaaca attgtttgct tttcatttaa gaccgttggc acatggatat
    36481 gccctaaagc ccaataatcg tatccctttt cttgcatctt tgagggttgg aaaggagcat
    36541 agtttccttt ttgagagacg ccaggttccc cgtggtacaa cccaatatgg taatctgtga
    36601 gttctctaga aggaaattcc atcactttgt ctttttgaat ccacggttga cgataactaa
    36661 atccactgag agagacagtt tctccacttt ttatcgttat tttttttgtc tctaccgttt
    36721 cggatgtaaa taaatggaca tttttaggaa aggagaacca ataccgctct ttttgataga
    36781 aatcatgatt tccgaatatt ataaatacag gtatatggtt cttttctaac cgtttcatct
    36841 gttctgaaaa atgtttctgt atttttaatg aaggacgatt ttgatgaaaa ttatctcctg
    36901 caagtaaaac aaaatcaacg ttatttatta ttgcctgttc aatgatattt gataaaacgg
    36961 tcagattcgt cttcaatagt ttttcctgta cagttttatc aagagttgtc agtccttcaa
    37021 atgaccgatc catatgtaaa tctgccgcat ggataaattt caccattaaa aatttcctcc
    37081 tttttttctt ttatcatacc attttcaatg ttttttttca tcaataaccg aaaatttgtt
    37141 cgtattatgc aaagaaatta cgataaatgg ccgacttcaa aaaataagtt tattcgaaaa
    37201 aaagattgtg acatgatttt tgtcacaatc ctaaatatct atgttatatt cgcttcgttt
    37261 gacactgaag aagtactatt tgtactggaa tttatgctcc atttttccgt atttagaatt
    37321 ctatgttcta tactacctta attagtttat atggtaagaa ttctttcaaa tgcttgagat
    37381 actccgtctt tatcatttga aacagtgaca taatcagctt ttttcatgat ttcttgatga
    37441 ctatttccca ttgcaaaact aatatctgca ctttcaaaca tagataagtc atttaaacta
    37501 tctccgatcg cgattctcaa aggtttcttt aaattgtaga ttgattccaa acgatcaaac
    37561 accacacctt tagatacatt ttgaggtacg atttcaatat ttacaggtgc cgattcagta
    37621 acagacacat ttagcatctt tttcaaatgg ttaaatatat tagctttcaa ttgacgatta
    37681 ggaataaaaa taaaaaattt gtaaatgctg atagcatcct tttctataat ttcctcaata
    37741 ttattataag ggattgaaca aatagatttt tcatactcaa gctcatagtt tagctctttg
    37801 atagaaaatt cactccctac ctcgtgaaaa atttttttca ttttattttt ataattctga
    37861 gattcataac ttccgagatt tgtatatatt ttggttggaa atttcgatag gacagaatca
    37921 atttttttta catcattttt atcgaaggaa gactcaaata tctttttttc tccatcaaat
    37981 ccaacagaac catttaaccc tactgctggt atccttaaac cagatttttt gagaatatta
    38041 ttaatgtctt ctacttctct tccagaacaa atgaatggaa aacagtcttc cggtaaatgt
    38101 ttcattgttc ttgcattttt ttgactaatg ctctggtcag aattcaaaag agtaccgtct
    38161 aaatctatac caattagttt catttcatcc atgtctccta tacgttgatt ataaattcac
    38221 tgcttattgt aagtctaaat
//
LOCUS       DASJOX010000015        37214 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJOX010000015 DASJOX010000000
VERSION     DASJOX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.015"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..808
                     /locus_tag="U4A12_000734"
     CDS             59..808
                     /locus_tag="U4A12_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1773142.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="U4A12_000735"
     CDS             complement(1232..1402)
                     /locus_tag="U4A12_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1773143.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="U4A12_000736"
     CDS             complement(1596..1775)
                     /locus_tag="U4A12_000736"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773144.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..1961)
                     /locus_tag="U4A12_000737"
     CDS             complement(1785..1961)
                     /locus_tag="U4A12_000737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773145.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="U4A12_000738"
     CDS             2162..3274
                     /locus_tag="U4A12_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1773146.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="U4A12_000739"
     CDS             complement(3352..3645)
                     /locus_tag="U4A12_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN1773147.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="U4A12_000740"
     CDS             3813..4127
                     /locus_tag="U4A12_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN1773148.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="U4A12_000741"
     CDS             4148..6127
                     /locus_tag="U4A12_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1773149.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="U4A12_000742"
     CDS             6140..6466
                     /locus_tag="U4A12_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN1773150.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="U4A12_000743"
     CDS             6482..7000
                     /locus_tag="U4A12_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773151.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="U4A12_000744"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="U4A12_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1773152.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="U4A12_000745"
     CDS             complement(8436..9941)
                     /locus_tag="U4A12_000745"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN1773153.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="U4A12_000746"
     CDS             complement(10033..10623)
                     /locus_tag="U4A12_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1773154.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="U4A12_000747"
     CDS             10802..12448
                     /locus_tag="U4A12_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1773155.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="U4A12_000748"
     CDS             12497..13216
                     /locus_tag="U4A12_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1773156.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="U4A12_000749"
     CDS             13695..14642
                     /locus_tag="U4A12_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773157.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="U4A12_000750"
     CDS             14654..15574
                     /locus_tag="U4A12_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773158.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="U4A12_000751"
     CDS             15615..17072
                     /locus_tag="U4A12_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1773159.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="U4A12_000752"
     CDS             17417..18031
                     /locus_tag="U4A12_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1773160.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="U4A12_000753"
     CDS             18024..19748
                     /locus_tag="U4A12_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1773161.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="U4A12_000754"
     CDS             19745..21196
                     /locus_tag="U4A12_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773162.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="U4A12_000755"
     CDS             complement(21669..23822)
                     /locus_tag="U4A12_000755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN1773163.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="U4A12_000756"
     CDS             complement(23824..24870)
                     /locus_tag="U4A12_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773164.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="U4A12_000757"
     CDS             25020..26309
                     /locus_tag="U4A12_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN1773165.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="U4A12_000758"
     CDS             26322..29024
                     /locus_tag="U4A12_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1773166.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="U4A12_000759"
     CDS             29009..29866
                     /locus_tag="U4A12_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1773167.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29937..30836)
                     /locus_tag="U4A12_000760"
     CDS             complement(29937..30836)
                     /locus_tag="U4A12_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1773168.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31005..33176
                     /locus_tag="U4A12_000761"
     CDS             31005..33176
                     /locus_tag="U4A12_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1773169.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33244..33585
                     /locus_tag="U4A12_000762"
     CDS             33244..33585
                     /locus_tag="U4A12_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1773170.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33861..35615)
                     /locus_tag="U4A12_000763"
     CDS             complement(33861..35615)
                     /locus_tag="U4A12_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773171.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35626..36462)
                     /locus_tag="U4A12_000764"
     CDS             complement(35626..36462)
                     /locus_tag="U4A12_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1773172.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36464..37147)
                     /locus_tag="U4A12_000765"
     CDS             complement(36464..37147)
                     /locus_tag="U4A12_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN1773173.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12004 a         6542 c         7634 g        11034 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac agttcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctctgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgagtcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggtttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atgcttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    29941 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30001 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30061 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30121 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30181 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30241 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30301 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30361 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30421 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30481 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30541 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30601 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30661 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30721 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30781 atcgtgattt tcacaagtgt actcctttca gtgcaattgc agactaattg taccattttt
    30841 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    30901 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    30961 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31021 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31081 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31141 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31201 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31261 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31321 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31381 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31441 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31501 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31561 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31621 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31681 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31741 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31801 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31861 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    31921 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    31981 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32041 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32101 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32161 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32221 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32281 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32341 atcaagctgt tgcacaaagt ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt
    32401 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32461 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32521 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32581 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32641 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32701 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32761 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32821 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    32881 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    32941 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33001 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33061 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33121 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33181 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33241 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33301 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33361 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33421 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33481 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33541 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33601 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33661 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33721 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33781 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33841 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    33901 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    33961 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    34021 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    34081 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacattgc
    34141 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    34201 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    34261 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    34321 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    34381 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    34441 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    34501 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    34561 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    34621 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    34681 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    34741 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    34801 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    34861 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    34921 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    34981 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    35041 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    35101 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    35161 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    35221 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    35281 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    35341 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    35401 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    35461 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    35521 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    35581 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    35641 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    35701 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    35761 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    35821 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    35881 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    35941 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    36001 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    36061 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    36121 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    36181 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    36241 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    36301 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    36361 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    36421 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    36481 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    36541 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    36601 aagtatcagg cataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    36661 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    36721 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    36781 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    36841 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    36901 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    36961 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    37021 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    37081 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    37141 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    37201 agaagacgga gcaa
//
LOCUS       DASJOX010000016        35333 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJOX010000016 DASJOX010000000
VERSION     DASJOX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.016"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..728)
                     /locus_tag="U4A12_000766"
     CDS             complement(243..728)
                     /locus_tag="U4A12_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1773174.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    818..1163
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1208..1849
                     /locus_tag="U4A12_000767"
     CDS             1208..1849
                     /locus_tag="U4A12_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1773175.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1868..2503
                     /locus_tag="U4A12_000768"
     CDS             1868..2503
                     /locus_tag="U4A12_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1773176.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2521..3336
                     /locus_tag="U4A12_000769"
     CDS             2521..3336
                     /locus_tag="U4A12_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1773177.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3655..5091
                     /locus_tag="U4A12_000770"
     CDS             3655..5091
                     /locus_tag="U4A12_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1773178.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5280..7727
                     /locus_tag="U4A12_000771"
     CDS             5280..7727
                     /locus_tag="U4A12_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1773179.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7957..9024
                     /gene="rlmN"
                     /locus_tag="U4A12_000772"
     CDS             7957..9024
                     /gene="rlmN"
                     /locus_tag="U4A12_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1773180.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9217..10320)
                     /locus_tag="U4A12_000773"
     CDS             complement(9217..10320)
                     /locus_tag="U4A12_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1773181.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10873..11421
                     /gene="aac(6')"
                     /locus_tag="U4A12_000774"
     CDS             10873..11421
                     /gene="aac(6')"
                     /locus_tag="U4A12_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1773182.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11967..13628
                     /locus_tag="U4A12_000775"
     CDS             11967..13628
                     /locus_tag="U4A12_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1773183.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13698..14045)
                     /locus_tag="U4A12_000776"
     CDS             complement(13698..14045)
                     /locus_tag="U4A12_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN1773184.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14129..15070
                     /locus_tag="U4A12_000777"
     CDS             14129..15070
                     /locus_tag="U4A12_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773185.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15071..16114
                     /locus_tag="U4A12_000778"
     CDS             15071..16114
                     /locus_tag="U4A12_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773186.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16120..17190
                     /locus_tag="U4A12_000779"
     CDS             16120..17190
                     /locus_tag="U4A12_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773187.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17180..18133
                     /locus_tag="U4A12_000780"
     CDS             17180..18133
                     /locus_tag="U4A12_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1773188.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18145..19155
                     /locus_tag="U4A12_000781"
     CDS             18145..19155
                     /locus_tag="U4A12_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1773189.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19440..20261)
                     /locus_tag="U4A12_000782"
     CDS             complement(19440..20261)
                     /locus_tag="U4A12_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1773190.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20358..20636)
                     /locus_tag="U4A12_000783"
     CDS             complement(20358..20636)
                     /locus_tag="U4A12_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773191.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20904..22913
                     /gene="metG"
                     /locus_tag="U4A12_000784"
     CDS             20904..22913
                     /gene="metG"
                     /locus_tag="U4A12_000784"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN1773192.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23392..23979)
                     /locus_tag="U4A12_000785"
     CDS             complement(23392..23979)
                     /locus_tag="U4A12_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773193.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24154..25476
                     /locus_tag="U4A12_000786"
     CDS             24154..25476
                     /locus_tag="U4A12_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1773194.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25631..26407
                     /locus_tag="U4A12_000787"
     CDS             25631..26407
                     /locus_tag="U4A12_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1773195.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26400..26969
                     /gene="rnmV"
                     /locus_tag="U4A12_000788"
     CDS             26400..26969
                     /gene="rnmV"
                     /locus_tag="U4A12_000788"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN1773196.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26962..27846
                     /gene="rsmA"
                     /locus_tag="U4A12_000789"
     CDS             26962..27846
                     /gene="rsmA"
                     /locus_tag="U4A12_000789"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN1773197.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28297..28926
                     /locus_tag="U4A12_000790"
     CDS             28297..28926
                     /locus_tag="U4A12_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773198.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29050..30159)
                     /gene="ugpC"
                     /locus_tag="U4A12_000791"
     CDS             complement(29050..30159)
                     /gene="ugpC"
                     /locus_tag="U4A12_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN1773199.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30368..30790
                     /gene="mgsA"
                     /locus_tag="U4A12_000792"
     CDS             30368..30790
                     /gene="mgsA"
                     /locus_tag="U4A12_000792"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN1773200.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31113..31649
                     /locus_tag="U4A12_000793"
     CDS             31113..31649
                     /locus_tag="U4A12_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1773201.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31689..33452)
                     /locus_tag="U4A12_000794"
     CDS             complement(31689..33452)
                     /locus_tag="U4A12_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773202.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33455..35170)
                     /locus_tag="U4A12_000795"
     CDS             complement(33455..35170)
                     /locus_tag="U4A12_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773203.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11509 a         6288 c         7313 g        10223 t
ORIGIN      
        1 actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat ttttatatat
       61 gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta
      121 ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat
      181 atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat
      241 cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg
      301 gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga
      361 cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct
      421 aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca
      481 aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca
      541 tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt
      601 acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg
      661 actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca
      721 cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa
      781 tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt
      841 agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga
      901 aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac
      961 agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa
     1021 aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc
     1081 atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga
     1141 accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga
     1201 aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac
     1261 attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt
     1321 aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat
     1381 gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg
     1441 gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta
     1501 ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc
     1561 gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac
     1621 aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt
     1681 gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga
     1741 cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct
     1801 aacaatcata atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg
     1861 aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa
     1921 agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg
     1981 cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt
     2041 ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg
     2101 agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt
     2161 agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt
     2221 agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg
     2281 acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta
     2341 tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct
     2401 atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga
     2461 aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag
     2521 atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt
     2581 tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt
     2641 ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca
     2701 ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt
     2761 cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata
     2821 tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat
     2881 atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa
     2941 atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa
     3001 caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt
     3061 aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta
     3121 tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt
     3181 tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa
     3241 aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat
     3301 aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga
     3361 cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg
     3421 agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta
     3481 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc
     3541 gaaactaagc gactttttca caacctcctt tacataattt tattttgttg tggaaggggt
     3601 ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa
     3661 cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat
     3721 caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta
     3781 cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat
     3841 cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct
     3901 gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct
     3961 ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta
     4021 tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag
     4081 atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc
     4141 cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca
     4201 gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat
     4261 ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga
     4321 ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct
     4381 gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa
     4441 tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct
     4501 ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa
     4561 acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca
     4621 ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat
     4681 ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga
     4741 ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga
     4801 gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat caattactta
     4861 caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat
     4921 tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga
     4981 tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac
     5041 agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac
     5101 ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa
     5161 attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa
     5221 aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa
     5281 tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa
     5341 aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg
     5401 tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca
     5461 ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg
     5521 tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct
     5581 tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg
     5641 aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag
     5701 tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct
     5761 tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc
     5821 tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag
     5881 tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag
     5941 agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg
     6001 cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata
     6061 ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa
     6121 tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac
     6181 caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct
     6241 cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg
     6301 atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa
     6361 atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag
     6421 ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa
     6481 gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga
     6541 tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca
     6601 gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag
     6661 aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc
     6721 ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta
     6781 tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga
     6841 aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga
     6901 accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg
     6961 aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga
     7021 aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg
     7081 aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag
     7141 cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg
     7201 atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa
     7261 gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag
     7321 gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca
     7381 tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt
     7441 atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac
     7501 aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt
     7561 cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac
     7621 tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg
     7681 agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg
     7741 aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa
     7801 tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga
     7861 ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt
     7921 atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc
     7981 ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct
     8041 gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat
     8101 ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag
     8161 atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa
     8221 aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca
     8281 caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt
     8341 gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag
     8401 cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat
     8461 aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt
     8521 ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct
     8581 aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga
     8641 acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat
     8701 gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta
     8761 aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta
     8821 gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt
     8881 ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg
     8941 atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa
     9001 atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg
     9061 agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa
     9121 agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt
     9181 gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg
     9241 ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc
     9301 tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat
     9361 tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt
     9421 ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa
     9481 ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac
     9541 agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac
     9601 agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg
     9661 acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca
     9721 catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga
     9781 aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc
     9841 aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa
     9901 gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga
     9961 acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac
    10021 ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac
    10081 actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag
    10141 actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga
    10201 aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt
    10261 tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat
    10321 attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac
    10381 tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca
    10441 tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa
    10501 acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt
    10561 aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat
    10621 tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt
    10681 tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa
    10741 tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
    10801 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
    10861 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
    10921 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
    10981 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
    11041 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
    11101 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
    11161 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
    11221 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
    11281 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
    11341 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
    11401 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
    11461 taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga
    11521 agaagctact tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt
    11581 agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc
    11641 gagaaactgg cttttgaaca ccgtttattc agataaggga ctgtgacaag acttttgtca
    11701 cagtttcttt ttgtttattc ttttttatct ttgtatggca ataatagaca atataacgaa
    11761 aattatttaa tggcttttgg aagaccatgg aaaaccttgt acaaagctat tttttctgaa
    11821 aaattccttg aaacctcacg aaaaaatcat tattttatga aaaagacaaa aaaaagtgtt
    11881 gattttttaa taattattca ttaaactctt aaacaacttc agaaaatatt aattattctg
    11941 aaaattccaa taaaggggta cgtaaaatga aaaagaaatt tacatttggt ttggttgcgg
    12001 tatgcggcct tgtacttgct ggatgttatg gcggcagttc agccacaagc agtagttcag
    12061 caaaaagcgg ttcagcagat ggaggaggcg tatttaactt agtcgtgcct caagaaatgc
    12121 ctacagctga cttatcagtt gcaacagata caatcagttt tacagcattg aataacgttt
    12181 atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca gccggcagga gctagcgagt
    12241 tggcagaagt cagtgaagac ggattaactt acaaaatcaa attacgagaa gacgcaaaat
    12301 ggtcaaatgg tgatcctgtt acagcagcgg attacgttta tggctggcaa cgaacagtag
    12361 acccagctac agcttctgaa tatgcttacc tgtatgcgcc agttgaaaat gcagaagaca
    12421 ttacagctgg aaagaaagat aaatcagaac tagggatcaa agcagttggt gactatgaat
    12481 tagaaatcaa attgacgaaa caaacaccat atttccaata tctgcttgct ttcccatcat
    12541 ttttcccaca aaaacaatcc gtagtggaag aaaatgggga tgcctatgca tctgcaagtg
    12601 ataaagctgt ttataatgga ccattcacat tagaagattt tgatggacca ggaacagata
    12661 cagaatggac ttataagaaa aatgatcatt actgggacaa agatacagta aaactttctg
    12721 aaatcaaagt cagtgtagta aaagaatctt ctactgcatt gaacttattc aaagatggcc
    12781 aagcagatga cgtgatcttg tctggtgagt tagcacagca aaacgcaaac gatccagctt
    12841 atacttctgt taaagaagcg cggacaagct atatcgaatt caatcaacgt gaaaagaatt
    12901 caccattcaa caatgtgaac ctaagaaaag ctatttctta ttctatcgat cgtgaagctt
    12961 tagtaaaaca agtactaggt gacggatcag ttgtctctac aggattgatc ccatcagata
    13021 tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga agctggaaaa ttagtttctt
    13081 atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa aaaagaattg ggcgttgatt
    13141 ctttagaatt tgatttgatg gcttctgatg atgattcatc gaaaaaagta atcgaatatg
    13201 ttcaaaactc aatccaagaa aacttggatg gcgtaacagt caaaccaaca cctgttccat
    13261 tctctgtacg tcttgaccgc tcaacttcgg gtgattttga cgcagtacta ggcggatggg
    13321 ctgctgacta tgcagaccca agcagcttta ctgatttgtt tgtcacaggt aattcataca
    13381 accgtggtcg ctggagcaat gcagaatatg acgaagcagt taaagcagct tcatcaacag
    13441 acgctggcga tgaacaagcc cgttgggatg atttccaaaa agcaatcaag atcatctctg
    13501 atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg tcacctagtg aatgaaaaaa
    13561 tcaaaggtat cgttcaccat gcggcaggcg cttcatggga ttacaaatgg acatacgttg
    13621 aagaataatc gttaataccg tgagcatgag aaaaaacgag gctgcggcaa atgtcgcaac
    13681 ctcgttctca tattcactta tcctaacaaa gaaaaaagca tgaaaatagc agaaagagca
    13741 attagtattc cggcaatgat cagccaaaag ctgtacatac cgtagctggc agacgagagc
    13801 gaggaaaatt ctggtttttt ctttcggttc cttgttcgtg caagatggac cgatcgctga
    13861 aaatgatcga agaaacccga agaaaagacc cataaaaaac cgccgataat cagaaacggc
    13921 aatgaccata agaatagatc attagataat tgaatgagtg taaaggaatg gttgatgaga
    13981 ttttttatta aaataaccag aatcagtaga aaagctactg cgtaggggat agattttttt
    14041 ttcatttgat tgctccttta tttttggttc attatctaat aatgtagcga aacatctgtc
    14101 agaagtcaaa agctaggggg aaacattagt gaatagttat atcaaatatg tattaaaacg
    14161 tgtctttttc atgatcatca cgctttggtt aattgcaaca atcacctttt tcttgatgca
    14221 attgctgccg ggcacgccat atacgaatca ggaacgtttg agtcctgaaa caatcgagat
    14281 gctaaataaa caagtcggtt tggataaacc agttatcgta caatatggga tttatctttc
    14341 taatttgctt caaggagatt ttggaatttc cttccaattt aaaaaccagc cagtcgcaca
    14401 tttgttagct ggaagaatcg gaccatcctt gcagttaggg ttacaggcaa ttatctttgg
    14461 aacagtccta gggacgattt taggaacgat ctctgccatg aagcaaaata catgggcaga
    14521 tacatcttct acactcgtag cgatattagg tcgttccatt ccaaactttg tatttgctgt
    14581 tttattacaa tatatctttg caatcaaatt acaagttttg ccaatcgcaa aatgggatgg
    14641 ctttgtgtac acaatcttac caacaattgc gttggccatg tcgccgcttg ctgactctgc
    14701 tcggtttatc cgtacggaaa tggtggaagt actgcatagt gattatgtgg aattagcaaa
    14761 agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat ggtcttcgaa atagtttgat
    14821 tccattgatg actttattag gaccgttagc tgtagcattg atgaccggat cactggtggt
    14881 agaaaatatc tttgctattc ctggtatcgg ggaacaattc gttaagtcaa tcacaacaaa
    14941 tgattatcct acgatcatgg ctgtaacgat cctttactca tttatgctga tttttgttat
    15001 tttgatcgtg gatctattgt acggtctagt tgatccaaga attcgtgttt cagaagggag
    15061 ccgaggataa atggaaatga taccaaagcg ttatgaaaca gttgcagata tccctgcgga
    15121 tgaatttctt ccattacaaa aaaataccga agaagaacgt gaacagatta ctgcaccttc
    15181 tttgaatttt atccaagatt catggcgccg tttaaagaaa aacaaagctg ccgttatttc
    15241 aatggtttta ctattggtca tcattttgat ctctgtgatc acaatctttg tttctcctca
    15301 tgatccaact gcccaaaacg tggcttatat caatttgcct cctcgtattc cgggaatcaa
    15361 tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa cttgttgata aatatgctca
    15421 agcaaacgtc ccttcaaacg taaatttctt tttaggaaca gacggtttgg gacgtgatgt
    15481 attaagccgc ttatttatgg gaatgagaat ctcgttgctg atcgcattta ttgctgcatt
    15541 actagatgta acgattggtg tagcttacgg gttggtttct ggtttgttag gcggacgtgt
    15601 cgataacgtg atgcaacgtt ttttggaaat cttatcagga attccaaacc tcgttgtcat
    15661 gattttgatg ttggtcgttt ttgaaccagg aattttttca atcgtggctg ctatggccat
    15721 cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa acaatgaaat taaaagatca
    15781 agaatatgtt ttagctggat tgacattagg tgaatcaaga atgaaaattg cattcaaaca
    15841 tattttgccg aatatctcta gtgtcattat tattcaaatg atgtttagta ttccatctgc
    15901 tattttcttc gaagcctttt taagctttat cggtttaggt cttacgcctc cgtcagcttc
    15961 attaggaacg atgttgagcg aagggtacaa gacattcctg tatctgcctt atctattatg
    16021 gattccggca gtaactttat cggttatcat gattggcttt aacttacttg ctgatggatt
    16081 gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga tgacgaaaat tttagaagtg
    16141 aaagacttag aaatctcttt tgatacgtat gctggaaagg tacgagcgat tcgcggtgtc
    16201 aattttcatt tgaataaagg agaaacattg gcgatcgttg gggaatctgg aagtggaaaa
    16261 tcagtgacga ccagaagcat catgcgcttg ctgtcaagca acgccaatat agatgctggt
    16321 caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag aaagagagat gcaaaaaatc
    16381 cgtggtaaag aaatagcgat gattttccaa gatccaatga cctctttaga tccgacgatg
    16441 cccattggga agcaagtcgc tgagtccctt agaaagcata ataaagtttc aaaaaaagag
    16501 ggacaaaaag ctgcattaga attgcttaaa ttagttggaa tacccgaagc tgagaaacgt
    16561 atcaacagtt atccgcatca gttctcagga ggtcagcgtc aacggatcgt gatcgcgatt
    16621 gcactggtat gttatccgga aatcctgatt gctgatgaac ctacaacagc gcttgatgtg
    16681 actattcaag cacagatttt ggagctgctg aaagaaattc agacaaaaat cgatacatca
    16741 attatcttta tcactcatga ccttggtgta gtagcgaatg tcgctgatcg tgtcgctgtc
    16801 atgtatggag gaagaatcgt tgaagttgga acatcggaag aaattttcta caacccgcaa
    16861 catccctata cttggggact gcttggttca atgccgacat tagatagtgc gaacgatcga
    16921 ttgtatgcta ttcctggttc tccaccggat ttattgaacc cgccaaaagg agacgcattc
    16981 tatccaagaa atgaattcgc gatgaaaatc gatgcagaac aagaaccacc attttttgaa
    17041 ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc cgcaggcacc caaagtgaca
    17101 ccgccagaag aaatccaacg tcgctgggct atctttaaag agaaacaaaa acatacttac
    17161 ggtcaaggag gaattgctga tgccaaataa actacttgaa gttaaaggat tgaaacagta
    17221 tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat gacatcagct ttcacattta
    17281 tgaaggagaa acattcggtt tagttggaga atctggaagc ggaaaatcca ctactggccg
    17341 tacgatcatt cgcttgaatg aacctactga tggtgagata ttattcgacg gtcaagatat
    17401 aacgaagatc aaagataaaa aaggtttgac gaaattccgg catgatgttc agatgatctt
    17461 tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc agagatatca ttgccgaagg
    17521 aattgatgtt aatggactag ctaaatcatc tgaagagcgt gcaaaaaaag tcgatgatct
    17581 attgagaact gttggattga atccaagcca cggtactcgt tatcctcatg aattttcagg
    17641 tgggcaacgc cagcgtattg gtattgcacg agcattggca gtaaaaccgc gttttatcat
    17701 ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa gcccaagtgg tcaatttgtt
    17761 gcaagatctc caaaaagaac atcaattgac ctatctattt attgcacatg atttgtcgat
    17821 ggttaaacat attagtgacc gaatcggtgt aatgcataac ggcttgttat tagagatggg
    17881 aacaagtgat gaaatctata atcacggtgt tcatccttat acagaaagtt tgttatctgc
    17941 tattcctcta ccagatccag accatgagag aaaacgcaga agaatcaaat atcaacctga
    18001 accagatgac ggacaagtac gtcaacttcg tgaaatcgca ccagaacatt ttgtctatgc
    18061 aactgagcag gaagtcccat attacgcaaa aaaactaaaa cgacaaaagg aatctcttct
    18121 ggttgctaac taggagggac atagatgcta aaagctttta aattccgtat gtatccgaca
    18181 gaagaacaaa aacagcaatt gattcgtact tttggatgtg ctcgatttac ctataaccat
    18241 ttgctgaaaa agcggcagaa aagttggcaa caaacaggag tagccaattt ttcgctcaca
    18301 cccgctacat taaaaaaaga atacccattc ttaaaagaag tagacagtct tgctttagca
    18361 aatgcacagc ttaatttgga tcgggctttt cgcaattatt tcaagggacg tgccagcttc
    18421 ccaaagctta aaacgaaaaa aagtatgtgg caatcttata cgaccaacaa ccaaacccgt
    18481 acgatctatc tggaaaatgg ctacttaaag ttaccaaaac aaaaagaact aatcaagata
    18541 aatcagcatc ggccagttga aggatcgatt cgctccgcta cgatttctgc tcgatataat
    18601 gaagaatttt atgtcgcact actttgtgat gtttcgccag taaaaaaaga atctttagct
    18661 aaatggatcg ggatcgccta tcatccgaag acattgatcc aaacatctcg acctctagaa
    18721 gtaactttac caaaatttca ccagacagaa gaaaagctgc aacatgcaca gcggaaattg
    18781 aatgtgaaag tccgctccgc tcaccataga aagatcaggt tagatcaggc aagcaattat
    18841 cagaaacaaa agagaaaagt aatggatctt tatctgaaac aaaagaacca gcgagaagat
    18901 tatcttgaac aattatctgg gaaattggtc aaacaatatg actacttatt tgtagaatct
    18961 tttccaaaag aagaggcaca tgctgatttt tctattcacg actggcataa attaataaca
    19021 aaattgcgtt ataagtccca atggtataat aaaaaatttc ttttgatcaa taccgacgga
    19081 gcagaagaaa gcaattccgt aagaaagagc caagtagtag aaaaaatggg cagacattcg
    19141 gttatcaagg gataaaaaat gaatgaaact gataggagcg ttagggttag ccttgttaat
    19201 aaaaagtctt aatgactttt gtttctagga atttttcttt tgaagaaaag ctcactaatt
    19261 atgacaaacc aataaaacaa tagcagaaaa agttcgtacg agctttttct gctattgtta
    19321 attttttatt cagaaagttt acgaatacat caaaattctg aaacaaaaaa cgtaactcac
    19381 aaagagttgt ttttgcgttt tttcttgaat gattaggtct gaatcttttg ctcacaacct
    19441 tattcatggt gccccggctt gagctgatag atttttgaag gacggccgat tcctacagga
    19501 cgttcaccga tctcatcgaa atattggagc atagctttct taaaattgga atgatcgatc
    19561 gttttataat cgatccctaa gaatttagca aatacttttc gagcttccgt aatcgtgaaa
    19621 tctttaccta aaacttgtag aacttgcggc tcgtgttcca ttttattgac tacacggttg
    19681 aatgctttca agatgatttg actatggtca aaagccaaag tatctttgcc agaagactct
    19741 ccagtgacta aatcaagcga gatagccact tctccgctag ataaatggat cttgttacta
    19801 tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat ctccggcgat tagcggttct
    19861 tctccaataa aggctaagta gctgactgtg acgacccagc ctcgcggatc tcgattaggc
    19921 gtactgaatg tatgcagttg ctcaatattt tcttttgaaa taaccacacc agtttcttcc
    19981 tttgtttctc ttaagacgct ttctccagtt gactcatcct tttggacaaa gccaccaggc
    20041 aatgcccatg aatttcgata aggatgccct tttctttgga tcaacaaaac tttcagctga
    20101 tcttcttctt tgttatagca catcagtacg atatcgacag tcaaagaggg cttgtcatat
    20161 tctggacgtt cctgtttttg ataccagtta agaaattctt cttcacttgc tttatgttca
    20221 taatataatt tttcttcttc tttagaagca aaacggttca tctgaccacc tccattgaca
    20281 gtaaaagtac tttttacctt attgtagcag actctgaaaa aaaaccaagg atttacttga
    20341 aaaatctagg ttatcattta aatatgaaaa ataaatggaa gtatgagatt gaataaaaag
    20401 aaaagcagat aaaacggcaa tactttatac cgctcttcac gaatggcaaa gaagccacaa
    20461 ataacgatat atacgtcaat gataaaacgc aaaatccaag ctgctgtcat aaaactgttc
    20521 aaatgcccat ggaggttaaa ataaccatac atacgaatat aaaaatagct gaatattaaa
    20581 tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa gatgtttctt ctgcatgatt
    20641 gttccagtcc cttactaaga tagatagtct ttattatacc acgtgtgagg ggagaaataa
    20701 aaagccgttt tttagtagat atgaaacaaa acaaaaaccc gcatttattc aacctattca
    20761 tccttttaaa ggatagattg aacgtagata gaagtcgttt atccagtctt tttcattttg
    20821 aaaaaaagct aacagcatgc tttttttatg gtatactagc aaggaaacga attcgcatca
    20881 tgcgataaag gaggaagagc cccgtggcag aaaaggaaac tttttatatc actacgccaa
    20941 tctattatcc aagcggcaaa cttcatatcg gcaactcgta tactacgatt gcatgtgacg
    21001 caattgctcg ttataaacgg ttgatgggct ttgatgtgtt ttatctcact ggagtggatg
    21061 aacatggtca aaaaatcgaa acaaaagcag aagaactagg tgtaaaaccg caagaatatg
    21121 tagataaaat ggcggctgat gtcaagaaat tatggaaaac gcttgatatc agttatgata
    21181 aattcattcg cacgacagat gattaccata aagcagctgt tcaaaaaata tttgatcgtt
    21241 tattagaaca gggagatatt tacttaggtg aatatgaagg ctggtactct gtttctgacg
    21301 aagaattctt tacagaaaca caacttgcag aagtgtacaa agatgacgaa ggtaacgtga
    21361 tcggtggtaa agcgccaagt ggacacgaag tagaattggt aaaagaagaa tcatacttct
    21421 tccgcatgag caaatacgca gaccgtctat tggaatacta tgaagaacat cccgaattca
    21481 tccagccaga atctcgtaaa aacgaaatga tcaacaactt tatcaaacca ggattagagg
    21541 atcttgcagt ttcacgaaca acttttactt ggggagtacc agtcaagagt gatccgaaac
    21601 atgtggtgta tgtatggatc gatgcattat ccaattatat tactgcacta ggatatggct
    21661 ccgaagatga aagtctattc gaaaaatact ggcctgcaga tgttcagatg gtcggtaaag
    21721 aaatcgttcg ctttcatacg atctattggc caatcatgct gatggccctt gatttgcctc
    21781 tacctaaaaa agtatttggg catggctggc tattgatgaa agacggaaaa atgtctaaat
    21841 caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg ttatggttta gatgcactcc
    21901 gttattattt gcttcgtgct gttccgtttg ggtcagatgg tgtctttacg ccagaagatt
    21961 ttgtttcccg tgtcaattat gatctggcaa atgatctagg gaacttattg aatcgaacga
    22021 ttgctatgat caacaaatat tgtgatggac tcgttcctga ctatgcctca cttgtcactc
    22081 catttgatag tgaactgtct acgacggctg ctaacgttgt cggacgttac catgatgcaa
    22141 tggaaaaaat ggaattcaat acagcattgg ctgaaatctg ggtgctgatt tctcgtgcaa
    22201 acaaatacat tgatgaaaca gaaccatggg tattagcaaa agacgaagaa aagaaagctg
    22261 aattgaatag tgtcatgatc catttagctg aatctttgcg tatcgcagcg atattacttc
    22321 agccaatcat gacagaaacg ccagagaaga tcttcaatca actaggattg gactctgaaa
    22381 caatgaatct tgaaggatta cactttggcg aatttccttc aggaacaaaa gtagttgcaa
    22441 aaggtacacc aatcttccca cgtttagata tggaagaaga agttgctttt attcaagaaa
    22501 aaatgtctga aggtactcaa acaaacgaag atacagtcaa atgggatcca gaagaaacag
    22561 aacttgtttc aacgaaagaa aaacaaatca aatttgatgt atttgaaaaa gtagaactaa
    22621 aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc agataagttg ctgcaattcc
    22681 gactagatgc tggagacagc caagaccgtc aaatcttgtc aggaatcgct gaattttatc
    22741 ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt agcaaatctg aaaccacgaa
    22801 agatgcgtgg acaaatcagt caaggaatga tcctttctgc agaagcacca gatggctctt
    22861 tacaagtaat cgaagcacca aaatccatgc caaatggctc agaaatcgca tagaaattca
    22921 aagggggtat gacactagtt gtgtcatacc ccctttttcc gaaatttgag aagcaatttt
    22981 cgtaaattcc ttcttattac ttgaagccaa tcatataata cagaaacaga gaactatcca
    23041 aaataaatcc tagaaaaaga agtacgagct tcgctgattt ttgttaattt agaataatac
    23101 taataggaat cgtaaaaaca gaaaaaaagc ataatgataa agggtcgggc agattttaat
    23161 tgattcatct ggtactcttt tttattgtcc ttacattcat aaagtttgaa tgcaatgact
    23221 ttattacatc tatatttata tctaacagta gcgttgatgt gttaattata agttctatca
    23281 ctttatgttc aattagtttg tgctcttata tgtgtgattg aaaatataga taaaaaaaca
    23341 atcatcggct ttgacttcga tgattgtttt ctttattttg taaaaatcct atcacttgat
    23401 tttatggtaa ccttttattt ttgtcttcaa acggcttggt aagagagcag taagcagtgc
    23461 aaatgggatc gaaaagacat accagaaaat cgtacgtccg ataccataat gttgatagag
    23521 ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc aaggagccag ctataaacca
    23581 atgattatcc caatgcatga cttcactaag tgccatcact ccaaaaggat agaaataaaa
    23641 atagatcccg actaaggtca tccgccagtt ctttgtaaat gttccgatat tcaataaaaa
    23701 gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc ccaaccaaaa aggaatacaa
    23761 catcccccac aagatcatca caggaccttt gatcaatgca acgattgcga tgaacaaagt
    23821 cagcaatatt agtttttgtg ataacgttgc aacagtcatt aatccaaata aaattaatgt
    23881 gaaccaatgc caatgggact gctgttgttc tttacgggaa aaatcgaccc ggttggataa
    23941 ataatctgct aatttcggat aagaacgctt aaaatccata aactgttcct ccttcgtgga
    24001 aaaggtaggt ttagtatacg aaaaataaaa aagaaataca tccgtcttaa gagggagttt
    24061 ttcactgaaa gtggagaaaa aagaatggta aattaaaata ttctcagtta tactgaaaat
    24121 tataccaaat ttcaaagaag aaagaggatg ggaatgaaag gcaaactaaa acgagaaatc
    24181 aatctgtttg gcgcattagc taccgttatg ggaaccgtga tcggtgcagg cgtcttcttc
    24241 aaaactgctg ctgttactgc tagtactcag tctacaagtt tgacattgct agcttggctt
    24301 ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg ctgaattagc tacagcaatc
    24361 ccggaaactg gtggagctgt aaaatatatt gaagctgcgt atggaaaatt gcccagcttt
    24421 ttattaggat gggcgcaaag cttgatttac tttcctgcta atattgctgc attatcgatt
    24481 atctttgcga cacaattgac gaacttatta caattatcta ccgattatct tttgttgatt
    24541 gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt tagggacaaa agtcggtgct
    24601 tctgtacaat cagtgacgtt gattgttaaa ttaataccaa ttgctgtgat tgtcatatgg
    24661 ggattattga ccccaggaca aggaactgtc cagttatttc caatcgaagc tggaaaagat
    24721 gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa cgttgtttgc ttatgacggc
    24781 tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc cggaaaaaga tttgcctaaa
    24841 gcaattattc ttggtttgag ttttgtaaca gtcgtttatc tattgatcaa ttttgttttt
    24901 ttgaaaactt tgccgattga tcatcttgcc ggtaatttga atgcagcttc tgaagcatca
    24961 gacgtcattt ttggaggaat cggtggaaag ctggtcacta ttgggatctt gatctcggta
    25021 tacggtgcat tgaacggtta tacactaact gggatccgcg tgccttatgc gatggcttta
    25081 gaagacgagc tacctttcag taaacagctg acaaagcttt caaagaagtt tacggttcct
    25141 tatgttcctg cggttttcca gttagcagta gcttgtatta tgatgagcct tggttctttc
    25201 gactttttaa cagacatgtt gatttttgtg atgtggctat tcagtttact gatttttatc
    25261 ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac gtccatataa agtaccactt
    25321 tatccaatcg ttccgattat tgcgattctc ggtgctattt tcattttagg tatgacgatg
    25381 atgacgcaaa cgaaattagc attgatcggt ataggtgtta ccttgatagg tatcccagtc
    25441 tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata tttcaacaac agacagaaaa
    25501 agtacgaact aggtcctcta gatcttcgtg ctttttctat ttcaataaaa caagttcttc
    25561 tattttgatt tttgctactt ctgcaagtag tgcttatgtg ctaaagtaag aatgaaaagg
    25621 agagataaac atgatatttg attctcatac ccatttaaat gctgaacagt tcaatgaaga
    25681 tattccagaa acgattcagc gagcacaaga aatgggggta acgaaaatgg cggtcgtggg
    25741 cttcgatacg ccaacgattg aaaagtcttt gcaattaagc catgactatc caaatatata
    25801 cagtattatc ggctggcatc cgacagaggc tggcagttat acgaaagaaa tcgaaaataa
    25861 attacaagaa caattgactt taccaaaggt tgtagcatta ggggaaattg gcttggatta
    25921 tcattggatg gaagacccga aagaagtcca agataaagta tttcgcagac agattgctat
    25981 cgcaaaagaa atgaacctac caatcagtat tcatactaga gaagcacttg aagacacgta
    26041 tcaaatccta aaagaagaag atgttcgaga tatcggtgga atcatgcata gtttcagtgg
    26101 tgattatgaa tgggcaaaac gatttcttga cttagggatg catatttcat ttagcggtgt
    26161 cgtcactttc aaaaaagcat tagacgtcca agaggctgca gcaaatgtac caatcgaacg
    26221 attacttgtg gaaacagatg ccccttatct ggcaccagtt ccttttagag gaaagcgaaa
    26281 tgaaccaggc tatactcgtt atacagtaga aaagattgct gaacttcgga atcttccgac
    26341 agaagaagtg gctgcccaaa catggacaaa tgcgcaccga ttatttggtc taagcgaaca
    26401 tggataagat gaagatccaa gagattatag tcgttgaagg aaaagacgat acaagaaggc
    26461 tgcgggaagt agttgatgca gatacgatag aaacaatagg gtctgcaatc aatgaagaga
    26521 tcctgatgca aattgaacat gcccaagaga ctcgtggagt gatcgttttt accgatcctg
    26581 atttttcagg agaaaaaata agaaaaatca ttatggaagc tgttcctcag gcaaaacatg
    26641 cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg aagccttggt gtcgagcatg
    26701 caagtgatgc tgctatttta gaagcattga aaaaggtcgt gacgccagac atttcccaaa
    26761 cggctgtttc tgaaatcact agacaagacc ttttagacta tggtttaata gctggggcac
    26821 aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg catcggttac acgaacggca
    26881 aacaattgga aaaacgtttg aagatgtttc gtattacgcc gaaagaactc gcccaagcaa
    26941 tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa ttgcgacacc ctctagaaca
    27001 aaagagattt tattgaaaca tggtttttca tttaaaaaaa gtttaggaca aaattttctg
    27061 actgaaccaa atattttaag gaaaatcgta gaaactgcgc aaatagatga acatacgaac
    27121 gtgatcgaag ttggaccagg aatcggtgca ttgacggaac aattagccaa gcatgcaaaa
    27181 caagtcgtag catttgagat cgatgatcgt ttgatccctg tattggctga tacgatgcaa
    27241 ccctatgaca atgtgactat tgtacatcaa gatattttaa agaccgattt gtcaacagct
    27301 gttagagaaa catttcatga ggagcttccg ttaaaagtcg tagcgaattt accttactat
    27361 attacgacac cgatcatgat gcattttctt gaatcagatt tggttgtgga tgaactagtc
    27421 gtcatgatgc aaaaagaagt agctgatcgt atctctgctg aaccaggaac gaaagcttat
    27481 ggttcattat cgattgctgt tcaatattat atggaagcca gtttagcatt catcgtacca
    27541 aaaacagtat ttgtccctca gccaaatgta gattcagcga ttttgaaact gacaagaaga
    27601 gatataccgg cagtcgaagt gacagacgaa aaagcatttt tccgattgac aaaagcggca
    27661 tttcagcaaa gaagaaaaac gctttggaat aacttgcagc attcgtatgg caaagatgac
    27721 cagacaaaag catggttagc aaaaagcttg gaaacagctg gaatcgatcc aaaacgtcgt
    27781 ggggagactt tgtcattaca agaattcgcc gcattaagca atgcgatgtc agaaaatatg
    27841 cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct gtcctgcatc aagtaataag
    27901 aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt ttgggcttgt taccgcagat
    27961 gcaagctttt gaacaccgtt taagaaagag gttgtgggcc tgccgtttaa ctctaagtca
    28021 caaggaacaa tttcaaaaaa cagcttctca tgtttttgag ttagttggtc ttattacttg
    28081 atgcaagctt ttgaccacta ttatgccgaa caaggagatg tgacacaaat tttgtcacat
    28141 ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac tcattttgta
    28201 tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat actaagtcga
    28261 catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc agttcgaaaa
    28321 ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt catgcctaaa
    28381 atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt tattgcgatg
    28441 ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg tttcttttta
    28501 acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc gttgattggg
    28561 gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt tacttttgct
    28621 aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt agcagaaatg
    28681 ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta ttctcaagga
    28741 tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag tttagttgat
    28801 tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat ccccgtattt
    28861 acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa gaaaacgaca
    28921 tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat aaatcctcta
    28981 agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac agctctaaca
    29041 gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga agtggccttt
    29101 gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc ttgcatctac
    29161 ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag ctccaagtaa
    29221 ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa gggctacttg
    29281 ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt cataaccttt
    29341 ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat gtccatctgt
    29401 gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc caataaagcc
    29461 ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga tttgttggat
    29521 aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg tctgatcgtg
    29581 agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct cagcacgcat
    29641 ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga agacttttgc
    29701 atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag ataaagcagc
    29761 tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt ccacacgttt
    29821 tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat tatcaaaaac
    29881 agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac ggtctttagg
    29941 tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga tatcttccag
    30001 tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa caaaaacgat
    30061 aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt ctgcattgtc
    30121 atatttttta tatacgtttt tcagagccat ttctaccata atacgttcca cactttcttt
    30181 tttagtttta ttaactattt aaagtataaa tgaaagcgtt aactaacttc aatgtcatgt
    30241 tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta ataaatatcc
    30301 ataagaaata aaaactgata cactggagta aataagtatg ttaacaatat caaaaggagt
    30361 gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa aaacctatga tggtgaaatt
    30421 agcaacagcg tataaaacaa tattggaaaa acatgaactt tttgctaccg ggacgactgg
    30481 gcagaggatc acggaagcaa ccggcttgcc gatccatcgg tttaaatccg gaccgttagg
    30541 cggggatcag caaattggag ccatgatttc ggaggatcag ttagatttgg tgatttttct
    30601 tcgagacccg ctggctgcac agccccatga accagatgta acagcactta tccgtttatc
    30661 tgatgtctat gagattccgt tagctacgaa tatcggaaca gctgaggtgc tactaagagg
    30721 actggaagcc ggcttcctag attttcgaag aatcgtccat gaaatagaca agcatccact
    30781 ttcattttga tggataagcg tatggaaaaa taacgtttaa tgttcgccta tatgtagcta
    30841 taaaagctaa acaaactgat aatatgcgat ataatacgaa cgtattggat aatataaaca
    30901 ataatattag tttttcgtat ttgcagtaaa tgtgaaaata tgctatagtt tctgtgggaa
    30961 aagatcccaa aaataataca gagtagaaaa taaagcgtta agtgctaaga ggatgggatg
    31021 ttgcctcttg gacgaaggac gtaatgttcg cggttttatt ttcgcattcg ctgcatgata
    31081 cgatgtagca gctcttaaag gagatgctaa aaatgaagaa acaccggtta gacgcacgta
    31141 tgattgctat tatgggctta ttgattgcat tgatggtcac tttgtcccga ctggtagcaa
    31201 tcgagacccc atttattaaa atcagtgtca catttattcc acaagtgatc atgggcattc
    31261 tttttggtcc tttttggtca gggataggcg cagttttagc tgatttagta ggaatggccc
    31321 tcttctcgaa atctgcattc tttattggat ttacattgaa tgcatttatt gaaggagcaa
    31381 tctacggatt ctttttctat cgaaaagaga tcacttggaa aaatgccatt ctggctacgc
    31441 ttagtgtcac gctgatcatc aacttatttt tgacaccatt atggcttgcg ttgatgtacc
    31501 atgtgccatt gttcagctgg gtcgtttggg cgccccgcct gttaaaaaca gtaatatggt
    31561 taccgattca atcaattgcc atttattacg taggacgtag tattccttac aaaaaaattt
    31621 taagaagttt agcgatccac gctaaataaa aaacaagcag tcttcccatc ccagaagact
    31681 gcttgttttt attgtgctgt gaattgtgct tgatagagcc gtcggtaata accatctttt
    31741 tgaagcagct catcatgggt gcccatttcg acaatagctc cctgatccat cactaatatc
    31801 tggtccgcat ttttgattgt ggacaggcga tgggcaatca caaaactggt ttttccttcc
    31861 atcattgcta agaaggcttg ttggattttt ttctctgtca atgtatcgac tgagctggta
    31921 gcttcatcta agatcaacat agggggatta ctgatcatcg ttcgagcaat cgtcaacagt
    31981 tggcgttgtc catctgatat ctttaaccct tgaccgccaa tcttagtatc taatttttcc
    32041 ggcaaacgtt ctacaaattc atacatataa gaggctttta gggcatcatt gatttctgcg
    32101 tcacttgctt ccggattccc aaaacgaaga ttttctcgca atgtactgtc aaatagccaa
    32161 gtatcttgta acaccattcc aaaactttta cgtaatgtat cacgttggat agagcggatg
    32221 tcatggtcat cgactgtaat tgttcctttt gttgtgtcat aaaaacgcat cagcaaatta
    32281 acaagtgtgg attttccggc ccctgttttt cctactatag caatggtttg acctggataa
    32341 gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt cataagaaaa agaaacatga
    32401 tcaaaagaga cttttccttt gatatttttg ggttccaagg cataggcatc tggcaaatca
    32461 ggcttttctt ctggctgatc gatcaaatca aatgctcgtt ctagaccaga aaaagcggtt
    32521 tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt tagaaaactg acttgagtag
    32581 atcgtaaagc tggaaatcac accaacagtg attgacgaac cactcttcaa tgccaatagg
    32641 ccgccaacta aaccaatcga taaataagca aggtgatcta caaaacgaga agacgggttt
    32701 gtcaaggaag aagaaaattg tgcccgttgt cctttttcat atagatcagc atttagcagt
    32761 tcaaattgct gttgactagc ttcttcttgc tgaaaagctt tcacgatttt ctggttaccg
    32821 attcgttcgg tcacaaagcc cgagatatcc ccaataatcc tttgctgtct atcgaaattt
    32881 gtttgtgaag cacgagcaac gatccaactc acaaggaaaa taattggtgt agaacaaaga
    32941 acgaccaatg tcagaagtgg acttagacgc atcataaaaa taaacgaaag aatgatgatt
    33001 gccactccag aaaataattg attgaaggcc gcagaaattg caatggaaat attgtccata
    33061 tcatttgtaa atcgactgac gatatttccg tgggagtttt gatcataata acgaagtggc
    33121 aatcgattca gatgagcaaa tgcatctttt cttaattttg caacggataa gtaagctaac
    33181 cgattgctta acacttggat gagccattga ctgactacgt tgattaaaac gataccaccg
    33241 aatagaataa gtaaatggaa cagtcgattg aagcggacct gtccttctcc aatcatttga
    33301 tcgatacttt gaccaatttg taacgtcatc acaactgttg caatcccatt gatgattcct
    33361 agtatcacag ctagccaaat gtcttttgca taggcagtaa ggtaaggaat gaatcggcgg
    33421 agcgtctgca aggataattt ttttactttc ataactattc ttcctcctgt gatgcgacga
    33481 tttcctgata ttctgctgat tgagcaagta attcttcatg ggtaccctga gcagcaatct
    33541 taccttgatc catcactaag atatgatgtg cttcttgaat agagcggaca cgttgggaga
    33601 taataatcac tgtactgcca agataagtct gtagcgcctt acgcaaatcc aagtctgttt
    33661 gataatccaa cgcacttaaa gagtcatcta aaatcagtaa tgctggcttg gcaatcaaag
    33721 ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa atttttccca ttttccatga
    33781 cttttgtatc taaaccattt ggaagctgac ggacaaattc ttctgcctga gcgattttca
    33841 gagcttccca acattctttt tctgtggcgt ctttcttacc ccattgaagg ttttcacgaa
    33901 tagtcccggt aaatagtaca gaagtttggg gaacaagagc aatttgctga cggagatgat
    33961 ccagctgcca agaacgaaca tccattccgt ccattataac tgtaccggaa ctgacatcat
    34021 agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct tccggttgga ccaatgattc
    34081 caaggactgt atttcgcttc agctgaaatg taatattttg gagagaaaga ccagaatcag
    34141 gagtataacg gaagtcaaca tggtcaaaag cgataattgc ttcagaagaa aaatcgggag
    34201 tagtactgac ctcagcttca agaatcgaat tttctgtgtc tagcacctct ttcacccgat
    34261 ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt tgaaacaacg atcagtgcca
    34321 aaagcatttg attcatataa ttgatcaaag caagaacttg tccttgttgc aagctaccaa
    34381 tatttacttt gatcccgcca actgtcagca aacagatgat cccgacattc atgatcaagg
    34441 ttgtagcagg tgataatagg gcagagatat tggaaacacg taaataattg ttcgctagat
    34501 catttgtgct tttatcgaac ttctctgttt ctttttttgt ctgcgcaaat gcgcggatga
    34561 cacgaacacc gctaagattc tgactaacgt gttcattgag ctgatctagt ttttcttgca
    34621 ctttttggta gagaggaacg gtttttttga tgataaaaaa taaaatgatt gagaaaatag
    34681 gcaataatgc taagaaaaac agacctactt cccagtcgat cacaaaagcc atgaccactg
    34741 acccgatact taaaaacggt gctctgatca ataaacgaat gaccattgct aaagctagct
    34801 gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt atctgttcca aaatgattca
    34861 actcgttgtg agagaaagta ttgatttttt tgattagctg attacgtaac tctgtaccaa
    34921 atccttgtga agctactgaa gcatagtatt ggcaaatgat agcacaaatc agaccaatca
    34981 cagacatagc gaccatccat aagcccattt caatgatttt tcctcggtca ttcattttta
    35041 gtccattatc gatcaaggaa gccatcatta aaggcaggac aagttcgaag acagcttcaa
    35101 gaaatttgaa gaacgggccc aaaatgattt gcttacgata gtcttttgca taacgtaata
    35161 actgaatcat atgattcctc ctattattta tttgtccaca attgaaaaaa caagtactac
    35221 cacattgtag cttaggaaat ttagaatgaa aagagaacaa gccttttcat aaatgaaagt
    35281 tttattatga agaagcttta gaaattgaat agactatttt ttttaaaaaa aag
//
LOCUS       DASJOX010000017        34387 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJOX010000017 DASJOX010000000
VERSION     DASJOX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.017"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            342..1031
                     /locus_tag="U4A12_000796"
     CDS             342..1031
                     /locus_tag="U4A12_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773204.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1018..2226
                     /locus_tag="U4A12_000797"
     CDS             1018..2226
                     /locus_tag="U4A12_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1773205.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2520..3791
                     /gene="serS"
                     /locus_tag="U4A12_000798"
     CDS             2520..3791
                     /gene="serS"
                     /locus_tag="U4A12_000798"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN1773206.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="U4A12_000799"
     CDS             complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="U4A12_000799"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN1773207.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6329..7024)
                     /locus_tag="U4A12_000800"
     CDS             complement(6329..7024)
                     /locus_tag="U4A12_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN1773208.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="U4A12_000801"
     CDS             complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="U4A12_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN1773209.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8223..8414)
                     /locus_tag="U4A12_000802"
     CDS             complement(8223..8414)
                     /locus_tag="U4A12_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN1773210.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8435..9325)
                     /locus_tag="U4A12_000803"
     CDS             complement(8435..9325)
                     /locus_tag="U4A12_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN1773211.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9312..10079)
                     /locus_tag="U4A12_000804"
     CDS             complement(9312..10079)
                     /locus_tag="U4A12_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1773212.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="U4A12_000805"
     CDS             complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="U4A12_000805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN1773213.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="U4A12_000806"
     CDS             complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="U4A12_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN1773214.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="U4A12_000807"
     CDS             complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="U4A12_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN1773215.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14650..15033
                     /locus_tag="U4A12_000808"
     CDS             14650..15033
                     /locus_tag="U4A12_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773216.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15201..15959)
                     /locus_tag="U4A12_000809"
     CDS             complement(15201..15959)
                     /locus_tag="U4A12_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN1773217.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15977..16795)
                     /locus_tag="U4A12_000810"
     CDS             complement(15977..16795)
                     /locus_tag="U4A12_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1773218.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="U4A12_000811"
     CDS             complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="U4A12_000811"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN1773219.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="U4A12_000812"
     CDS             complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="U4A12_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN1773220.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17923..19257
                     /gene="dnaA"
                     /locus_tag="U4A12_000813"
     CDS             17923..19257
                     /gene="dnaA"
                     /locus_tag="U4A12_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN1773221.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19455..20585
                     /gene="dnaN"
                     /locus_tag="U4A12_000814"
     CDS             19455..20585
                     /gene="dnaN"
                     /locus_tag="U4A12_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN1773222.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20814..21056
                     /gene="yaaA"
                     /locus_tag="U4A12_000815"
     CDS             20814..21056
                     /gene="yaaA"
                     /locus_tag="U4A12_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN1773223.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21043..22167
                     /gene="recF"
                     /locus_tag="U4A12_000816"
     CDS             21043..22167
                     /gene="recF"
                     /locus_tag="U4A12_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN1773224.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22164..24110
                     /gene="gyrB"
                     /locus_tag="U4A12_000817"
     CDS             22164..24110
                     /gene="gyrB"
                     /locus_tag="U4A12_000817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN1773225.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24130..26601
                     /gene="gyrA"
                     /locus_tag="U4A12_000818"
     CDS             24130..26601
                     /gene="gyrA"
                     /locus_tag="U4A12_000818"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN1773226.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26837..27136
                     /locus_tag="U4A12_000819"
     CDS             26837..27136
                     /locus_tag="U4A12_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN1773227.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27186..27698
                     /gene="ssb"
                     /locus_tag="U4A12_000820"
     CDS             27186..27698
                     /gene="ssb"
                     /locus_tag="U4A12_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1773228.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27724..27960
                     /locus_tag="U4A12_000821"
     CDS             27724..27960
                     /locus_tag="U4A12_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN1773229.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28103..30076
                     /locus_tag="U4A12_000822"
     CDS             28103..30076
                     /locus_tag="U4A12_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN1773230.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30082..30534
                     /gene="rplI"
                     /locus_tag="U4A12_000823"
     CDS             30082..30534
                     /gene="rplI"
                     /locus_tag="U4A12_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN1773231.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30808..32175
                     /gene="dnaB"
                     /locus_tag="U4A12_000824"
     CDS             30808..32175
                     /gene="dnaB"
                     /locus_tag="U4A12_000824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1773232.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32955..34247
                     /locus_tag="U4A12_000825"
     CDS             32955..34247
                     /locus_tag="U4A12_000825"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN1773233.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10492 a         6689 c         6807 g        10399 t
ORIGIN      
        1 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
       61 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      121 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      181 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      241 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      301 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      361 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      421 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      481 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      541 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      601 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      661 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      721 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      781 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      841 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      901 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      961 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1021 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1081 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1141 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1201 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1261 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1321 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1381 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1441 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1501 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1561 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1621 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1681 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1741 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1801 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1861 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1921 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1981 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2041 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2101 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2161 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2221 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2281 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2341 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2401 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2461 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2521 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2581 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2641 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2701 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2761 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2821 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2881 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2941 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3001 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3061 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3121 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3181 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3241 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3301 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3361 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3421 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3481 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3541 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3601 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3661 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3721 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3781 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3841 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3901 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3961 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4021 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4081 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4141 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4201 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4261 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4321 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4381 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4441 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4501 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4561 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4621 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4681 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4741 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4801 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4861 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4921 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4981 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5041 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5101 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5161 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5221 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5281 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5341 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5401 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5461 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5521 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5581 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5641 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5701 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5761 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5821 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5881 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5941 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6001 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6061 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6121 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6181 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6241 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6301 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6361 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6421 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6481 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6541 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6601 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6661 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6721 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6781 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6841 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6901 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6961 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7021 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7081 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7141 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7201 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7261 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7321 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7381 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7441 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7501 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7561 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7621 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7681 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7741 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7801 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7861 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7921 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7981 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8041 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8101 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8161 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8221 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8281 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8341 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8401 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8461 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8521 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8581 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8641 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8701 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8761 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8821 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8881 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8941 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9001 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9061 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9121 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9181 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9241 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9301 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9361 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9421 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9481 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9541 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9601 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9661 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9721 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9781 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9841 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9901 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9961 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10021 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10081 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10141 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10201 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10261 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10321 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10381 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10441 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10501 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10561 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10621 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10681 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10741 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10801 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10861 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10921 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10981 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11041 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11101 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11161 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11221 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11281 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11341 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11401 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11461 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11521 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11581 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11641 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11701 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11761 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11821 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11881 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11941 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12001 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12061 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12121 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12181 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12241 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12301 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12361 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12421 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12481 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12541 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12601 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12661 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12721 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12781 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12841 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12901 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12961 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13021 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13081 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13141 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13201 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13261 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13321 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13381 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13441 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13501 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13561 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13621 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13681 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13741 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13801 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13861 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13921 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13981 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14041 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14101 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14161 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14221 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14281 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14341 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14401 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14461 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14521 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14581 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14641 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14701 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14761 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14821 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14881 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14941 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15001 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15061 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15121 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15181 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15241 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15301 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15361 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15421 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15481 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15541 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15601 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15661 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15721 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15781 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15841 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15901 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15961 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16021 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16081 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16141 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16201 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16261 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16321 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16381 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16441 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16501 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16561 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16621 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16681 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16741 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16801 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16861 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16921 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16981 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17041 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17101 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17161 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17221 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17281 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17341 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17401 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17461 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17521 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17581 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17641 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17701 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17761 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17821 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17881 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17941 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18001 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18061 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18121 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18181 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18241 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18301 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18361 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18421 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18481 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18541 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18601 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18661 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18721 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18781 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18841 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18901 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18961 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19021 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19081 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19141 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19201 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19261 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19321 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19381 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19441 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19501 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19561 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19621 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19681 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19741 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19801 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19861 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19921 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19981 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20041 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20101 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20161 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20221 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20281 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20341 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20401 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20461 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20521 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20581 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20641 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20701 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
    20761 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
    20821 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
    20881 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
    20941 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
    21001 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
    21061 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
    21121 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
    21181 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
    21241 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
    21301 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
    21361 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
    21421 agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
    21481 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
    21541 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
    21601 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
    21661 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
    21721 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
    21781 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
    21841 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
    21901 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
    21961 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
    22021 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
    22081 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
    22141 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
    22201 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
    22261 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
    22321 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
    22381 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
    22441 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
    22501 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgttaacg
    22561 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
    22621 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
    22681 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
    22741 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
    22801 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatta
    22861 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
    22921 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
    22981 attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga acacatgaat
    23041 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
    23101 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
    23161 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
    23221 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
    23281 aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc agagctcgtc
    23341 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
    23401 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
    23461 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
    23521 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
    23581 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
    23641 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
    23701 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
    23761 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
    23821 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
    23881 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
    23941 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
    24001 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
    24061 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
    24121 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
    24181 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
    24241 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
    24301 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
    24361 taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc acagccattc
    24421 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
    24481 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
    24541 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
    24601 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
    24661 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
    24721 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
    24781 tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta tgaaacaggt
    24841 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
    24901 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
    24961 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
    25021 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc tgttatcttg
    25081 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
    25141 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta tatcgaacat
    25201 cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga agcaagggca
    25261 cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
    25321 cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt cgatttatcc
    25381 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
    25441 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
    25501 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
    25561 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
    25621 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
    25681 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
    25741 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
    25801 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
    25861 gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc gagtgaaagc
    25921 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
    25981 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
    26041 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
    26101 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
    26161 gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct gcgtgaggaa
    26221 gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt gatcacagaa
    26281 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
    26341 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
    26401 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
    26461 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
    26521 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
    26581 gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa gaatgaccga
    26641 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
    26701 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca gagcttgtga
    26761 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
    26821 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
    26881 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
    26941 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
    27001 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
    27061 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
    27121 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
    27181 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
    27241 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    27301 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    27361 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    27421 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    27481 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    27541 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    27601 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    27661 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    27721 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    27781 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    27841 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    27901 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    27961 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    28021 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    28081 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    28141 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    28201 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    28261 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    28321 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    28381 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    28441 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    28501 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    28561 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    28621 tggtatttta tctattgata actatgcaga tgccattgat aaattggatg acaaagagat
    28681 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    28741 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    28801 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    28861 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    28921 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    28981 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    29041 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    29101 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    29161 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    29221 cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    29281 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    29341 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    29401 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    29461 tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    29521 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    29581 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    29641 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    29701 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    29761 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    29821 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    29881 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    29941 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    30001 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    30061 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    30121 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    30181 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    30241 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    30301 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    30361 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    30421 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    30481 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    30541 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    30601 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    30661 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    30721 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    30781 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    30841 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    30901 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    30961 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    31021 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    31081 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    31141 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    31201 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    31261 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    31321 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    31381 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    31441 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    31501 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    31561 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    31621 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    31681 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    31741 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    31801 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    31861 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    31921 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    31981 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    32041 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    32101 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    32161 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    32221 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    32281 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    32341 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    32401 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    32461 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    32521 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    32581 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    32641 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    32701 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    32761 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    32821 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    32881 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    32941 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    33001 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    33061 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    33121 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    33181 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    33241 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    33301 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    33361 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    33421 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    33481 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    33541 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    33601 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    33661 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    33721 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    33781 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    33841 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    33901 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    33961 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    34021 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    34081 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    34141 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    34201 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    34261 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    34321 acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34381 ttccgaa
//
LOCUS       DASJOX010000018        34100 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJOX010000018 DASJOX010000000
VERSION     DASJOX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.018"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1231
                     /locus_tag="U4A12_000826"
     CDS             5..1231
                     /locus_tag="U4A12_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1773234.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1319..1711)
                     /gene="rpsI"
                     /locus_tag="U4A12_000827"
     CDS             complement(1319..1711)
                     /gene="rpsI"
                     /locus_tag="U4A12_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1773235.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1727..2170)
                     /gene="rplM"
                     /locus_tag="U4A12_000828"
     CDS             complement(1727..2170)
                     /gene="rplM"
                     /locus_tag="U4A12_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN1773236.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2689..3156)
                     /locus_tag="U4A12_000829"
     CDS             complement(2689..3156)
                     /locus_tag="U4A12_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1773237.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3276..4814)
                     /locus_tag="U4A12_000830"
     CDS             complement(3276..4814)
                     /locus_tag="U4A12_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN1773238.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4894..5580
                     /locus_tag="U4A12_000831"
     CDS             4894..5580
                     /locus_tag="U4A12_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN1773239.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5781..6308)
                     /gene="msrA"
                     /locus_tag="U4A12_000832"
     CDS             complement(5781..6308)
                     /gene="msrA"
                     /locus_tag="U4A12_000832"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1773240.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6392..7069)
                     /gene="pgmB"
                     /locus_tag="U4A12_000833"
     CDS             complement(6392..7069)
                     /gene="pgmB"
                     /locus_tag="U4A12_000833"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1773241.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7066..8196)
                     /locus_tag="U4A12_000834"
     CDS             complement(7066..8196)
                     /locus_tag="U4A12_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1773242.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8196..10379)
                     /locus_tag="U4A12_000835"
     CDS             complement(8196..10379)
                     /locus_tag="U4A12_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1773243.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10376..11401)
                     /locus_tag="U4A12_000836"
     CDS             complement(10376..11401)
                     /locus_tag="U4A12_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1773244.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11446..12273)
                     /locus_tag="U4A12_000837"
     CDS             complement(11446..12273)
                     /locus_tag="U4A12_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1773245.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12270..13337)
                     /locus_tag="U4A12_000838"
     CDS             complement(12270..13337)
                     /locus_tag="U4A12_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN1773246.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13363..14199)
                     /locus_tag="U4A12_000839"
     CDS             complement(13363..14199)
                     /locus_tag="U4A12_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773247.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14196..15065)
                     /locus_tag="U4A12_000840"
     CDS             complement(14196..15065)
                     /locus_tag="U4A12_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773248.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15135..16418)
                     /locus_tag="U4A12_000841"
     CDS             complement(15135..16418)
                     /locus_tag="U4A12_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1773249.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16432..18117)
                     /locus_tag="U4A12_000842"
     CDS             complement(16432..18117)
                     /locus_tag="U4A12_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN1773250.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18392..19417
                     /locus_tag="U4A12_000843"
     CDS             18392..19417
                     /locus_tag="U4A12_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773251.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20012..23686)
                     /gene="rpoC"
                     /locus_tag="U4A12_000844"
     CDS             complement(20012..23686)
                     /gene="rpoC"
                     /locus_tag="U4A12_000844"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN1773252.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23744..27361)
                     /gene="rpoB"
                     /locus_tag="U4A12_000845"
     CDS             complement(23744..27361)
                     /gene="rpoB"
                     /locus_tag="U4A12_000845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1773253.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28089..29057
                     /locus_tag="U4A12_000846"
     CDS             28089..29057
                     /locus_tag="U4A12_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1773254.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29075..29980
                     /locus_tag="U4A12_000847"
     CDS             29075..29980
                     /locus_tag="U4A12_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773255.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29977..30783
                     /locus_tag="U4A12_000848"
     CDS             29977..30783
                     /locus_tag="U4A12_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1773256.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30919..31908
                     /locus_tag="U4A12_000849"
     CDS             30919..31908
                     /locus_tag="U4A12_000849"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN1773257.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32225..32749)
                     /locus_tag="U4A12_000850"
     CDS             complement(32225..32749)
                     /locus_tag="U4A12_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN1773258.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33036..>34100
                     /gene="scm"
                     /locus_tag="U4A12_000851"
     CDS             33036..>34100
                     /gene="scm"
                     /locus_tag="U4A12_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1773259.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9728 a         7247 c         6388 g        10737 t
ORIGIN      
        1 gattttgcct acaaagcttt caaatgggaa atataaaact aatctgagat atccaaaaag
       61 gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa aaaacatttc caaataggca
      121 attggcgatt aaagccgaaa atgatatgaa gaagaaaata gaaaaagttt tgagagagga
      181 gaatgcaaac tcattagaat taaaaggaaa aataacgttt aaaaaatttt acgaatctaa
      241 atggcttcct agatatgaat taggtcaaac aattagatca aacaggcctc ctagtgatat
      301 aaccattagt aatacaaaag atatatttcg gttgcatatt ttacctatgt ttggtgaata
      361 cgcaatgaat tatctaaata tcaatactga gataatatca gatgaattaa ccaaaaaatc
      421 gaaagaatat gcaaatataa aaatcataaa aggatatgtt cgttccatgt tcgatattgc
      481 agaaatacta aattatatcg aatttaatcg aactactaaa attattcaaa gtatcactgc
      541 acctaaaaaa aatgctttag aagaaaaaag aatacaagaa ggtaagcaag cactatcatc
      601 aaaagaactt actaattgga tagaagcagt aaatgatgat tttaataatc atttacttac
      661 ttttcatgat tacacacttt ttatgattac cctgtatctt ggtgatagaa aaagtgagac
      721 ttacgctttg caatggaaat atattgattt tgaaaaacaa acggtccgtc ttaaacatac
      781 tttagataaa tatcaaagaa aaaagtttac aaaaggtcga aaagataccg tcattcaagt
      841 accagaagtt gtaatgacat tgctaagtga atggaaatcg gtacaagctg atcaattgtt
      901 gaaattaaaa attaagcaaa cgttagatca gtatctcttt acttatacta aaccatctgg
      961 agaagtaaat tgtcctgttc atgccgatta cctaaactat cgaatcaatt caattaaaag
     1021 aaggcatcct gacttagttc atttatctcc acacaaactc cgtcatactt atgcaactat
     1081 agctagacaa ggtggagcag atatgaatca aatttcaaat gctttaacac actcagatat
     1141 ttctacaaca aagatatatg tgaacacacc agatatagtt gataaggctg tttttgaagc
     1201 ttttcagaga ggattgaata agtgtgattg atatgtgttc ttaaatgtgt tcttaaaaac
     1261 aaaagaagca ctttccaaat aattgaaaag tgccagaaac gttgatatat aaaggatatt
     1321 aacgttttga gaattgtgaa gctttacgtg ctttcttaag acctggtttt ttacgttcga
     1381 ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat
     1441 caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc
     1501 ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag
     1561 gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat
     1621 ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac
     1681 ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg
     1741 tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt
     1801 tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag
     1861 tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc
     1921 ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt
     1981 tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag
     2041 ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa
     2101 catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg
     2161 ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt
     2221 ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat
     2281 gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt
     2341 tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct
     2401 tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     2461 acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg
     2521 aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
     2581 tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg tttctctaat actcaggtca
     2641 aaacgctgat ttcacaacct ctttttcggt caagttcaac aacagttttt acgcgtcgat
     2701 ctctggcgct ttgcccaaat gagcttggat catccagata cgtttttctg tggctgtttt
     2761 gaattcgata aagatatctt gtgttggatc gtcgccttct tctccagaaa cttcgattcc
     2821 tttttggtaa agatctgcta gataacgata gcctgcaaca agtgtttcca aacgttctgg
     2881 aatagtacga tcccagcggc caatttcatc agggattttt gtgttatctg caaattcacg
     2941 caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt tcggatactt catctaactg
     3001 agcgttcaaa tcatccatga actcatccat cattgggtgc aatgtaagga aacctggtcc
     3061 tctcatgtac caatgtgttt ggtgaacaac aacagacatt tggcttagat ctgcaactaa
     3121 ttgatttaat acttctttgg tttgttggaa tttcatcttt cttgcctcct aacattttct
     3181 tacacttaaa ataatagtag actttcaatg aaaagtacaa caaaatcatt tacctgagaa
     3241 cgattttcag ttcgcttttt tatccgtaaa gccaatcatt tttcagaatc agctaattct
     3301 tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat
     3361 aatggataga ttttctgata ttttttatga tcagtttctt gtactccaac ttgctctttc
     3421 aattctatca attcctgtgc ttcactgata ctttggatct ttccgatgct tctccagcct
     3481 aaaatcacag ctcctaatgc ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc
     3541 agtacatcag ccatcatctg acgaaaaatc ggagattgag caaacccgcc tgtagcacgg
     3601 atctctgtga gctgattttc ctggcaaact ccttttaaaa tccgataaag gttgagacag
     3661 atcccttcta gaatcgcctt catcatttct ttttctgtat gttgccgctg taaaccgata
     3721 aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct caccaagtaa atagggatga
     3781 aaaatcagac cattggcacc cgctggcaca aaagcgatag ccgaaagcag tgcatcatat
     3841 ggattttctc ctttttcttt agcagattga acgacttcct gtaaaaatgt atgccctgcc
     3901 caatcaaaga tgccagcacc attgctagtc gctcctccca ctacccaatg tttctgatcc
     3961 agaacataac aaaacgtctc catttctgga tggaactgag gctgagtagt aataaaacga
     4021 agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct
     4081 agattagaaa gcggcccgtc tgcccctccc aaaacaaatg atatatcatt tggcaaacct
     4141 agttctgctg ctgtttttga tggtagacct gttacttgat aggtggaagg aaagacttgc
     4201 ggcaattgct ctattcgaat gttcaagtag tcaagcactt cttctgccca ttggaattca
     4261 tggatatcaa aatatccagt tcctgaagct gttccgtagt cgctgatcag ttcgccaaac
     4321 aatctataaa agatatagtc tttgataccg acaaaataag cagttttttt gaataattca
     4381 ggttggctgt tttgcagcca cttgattttg ttcaatggtg tcattggatg aatcggcgtc
     4441 ccagtctgcc gataaaattc tttgccaagc acggtatatt tcagtttttc tgcttcgtcc
     4501 gctgctcggt tatctgccca agtgatcatt cgagataacg gtgttttctc ttcatccaac
     4561 aatatcaggc catgcatcgc agaggagaag gagacaaatt gaatgattgt gtctgaaggc
     4621 aactgtttaa ttacttcttt tataacggtg cagacacctt gaaagatcaa ttcctgatct
     4681 tgttctgcca tcccttgtgt ttcttggatc aaagggtagc gaaaataagc ggatgctgct
     4741 tcttgggctt catctgtgaa agcaactgct ttggtttgtg tggttccgat atctaatccg
     4801 attgaaacgt tcattttttc acctcaaatt ttatgtatgt acaaaaattt agtttaagct
     4861 tgcgtatctt ggttttgaaa agggggagat ggcgtgttac tttattttat cattggttgc
     4921 tgtatcggat catttttatg tttaaccgcc cagcggattc cgttaggtca ctctattatt
     4981 tatcctagat ctcattgtgt caaatgctgt cgttcgcttt cttggtatga attgattccg
     5041 attctctcga tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc
     5101 tactatttgt tggcagaagc cttatgtggt ggtctttttg cctggttctt tactttttct
     5161 tccgcaagaa atttttcgac gttcatatgg atgctttcgg ctttgctatt ttcgttgatg
     5221 gatttatttt acttcatggt ccattctcac acgctattct gttcttgggc aattttatgg
     5281 attttttggt tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta
     5341 ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag ctggcgatac tctgttgatg
     5401 ctttcttgga gtggtggctt gtctctagaa gagttgcttc agatcttgtt tcttgctagt
     5461 ctgtttggaa tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag
     5521 ctgcctttca ttcctttttt gagcagtgcg ttgctgattg tccttcatct taggtattga
     5581 aaaaaggttg tgacaaacga cttgtcacaa cctctgatat atagtgttct cttaccaaat
     5641 ccttgtgact tggagctagg cgacaggttc acaacctctc cactaaatgg tgttcaatca
     5701 gctgaccctc agtattagct caaaatgctg gttttacaac ctctgatata cggtgttcca
     5761 taagtagctt tttcggcttc ttatttcttg acgccccaat gtcgacgaag gaaacctgca
     5821 cgtccagaac cttctcgata aagattgtaa tggagcttgt ttttcttgta ataatcctga
     5881 tggtaatctt ctgcaggata aaattcgctt cttggttcga tttttgtcac gattggctta
     5941 tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt tcgttcttct
     6001 tcatcattat agtagatcac tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat
     6061 gcgtcagtcg gatcggtttg ctgccaatag atttccacca actgatcata agaaataact
     6121 tctggatcat attcgatctc tactgcttct gtatgccctg ttgtgcctgt tgttacttgt
     6181 tcataagttg gatttggtac atgcccgcca gtataaccgg atgtcacaga aatgatccct
     6241 ggctgctctt caaatggttt gaccatacac caaaagcatc cgcctgcgaa aactgctttt
     6301 tttgtcatat aaaatccccc ttttttctca tcataccttg atttccaaaa ggaggataac
     6361 gttttgcttg tgacagaaaa aacggaaact attatatacg tgatacggct tctgataaat
     6421 gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag
     6481 gaagatttat gttttcacct attcccaata catgcatgtt accagccaaa gctgcttgac
     6541 aaccagcttc tgaatcttca atgaccaagc atgcatttgg cggtacattc aattgctgtg
     6601 ctcctttgag aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa
     6661 ggacatcaaa ataacttgtc aaattcgttt tttctaaaat gaggcgtgca tttttgcttg
     6721 ctgatcccaa tccgatcttg atgtttgttt ttttcaagta ctcaagaaca gctattgcac
     6781 caggcaaaac ggatgtttca tccatttttt gaatcgcttg aagatataga ttgttttttt
     6841 gctcagcaaa ggctgatttc tctttttctg agaatttctg ttccattttt ccaaatgaca
     6901 gcaatcgctc tagtgactcg gttctggata ttcctttcag ttgctcgttt tctttctcag
     6961 tgaatggaat ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga taacgagctg
     7021 tgtctacaag aacgccatct aaatcgaata gtgctgcttt gattttcatc gattacctcc
     7081 ttgaaaatga acgacttgaa gtaaagatgc gatgttttta gctgtgcgat gaagattatc
     7141 agtggcttgc tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc
     7201 cgaaatgcca acatctgctg ctttttcgct tcctttacca acacttccgc aaatagctat
     7261 cacaggaacg ccctctggta ctcttctagc aatcccaact ggcgcttttc cttttagact
     7321 ttgtccatct agacgtcctt cgcctacgat tacgaggtct gcttgctgta cccgtttatc
     7381 gaactccagt aaatccaata cgtagtcgat gccagagact attttccctg aagcaaatgc
     7441 taacagtcct gcacccattc caccaccagc actactgccc gatgcctcga ttacttgagg
     7501 ggcaaatctc atataaaaat catgcatggc ttggtcagcc actgctaatt cattttcttt
     7561 caagcctttt tgtggtccga aaacaaaaga agcgccttct tttccgcata aaagattagt
     7621 aacatcagta atgacagtga ttttggcttt aatcagctca ataggcacgt tcgttttatc
     7681 aactgtagca attttaccta gattttctcc cagtgcttct actttttgat tatcttgatc
     7741 gtaaaaagca taacctagac cttctgccat gccgatgccg ccatcgttgg tactacttcc
     7801 gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc
     7861 tactcctcgc gtggataacc taagtggctg ttgttggttt agtgggacat ctttcaatcc
     7921 gacgatttct gccatttcaa aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt
     7981 gtttttgcca aatgcattag taacgactgc tgtttgccgc ttcaacctta aactgtctgc
     8041 taatgatgaa accgttcctt ctcctccatc gcctataggt agcagatcat attcagcctg
     8101 aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct gcctctaagg ctgtcagact
     8161 ttctttgaaa gaatctggcg caatcagaat acgcattatt ttcccctccg ttccaagatc
     8221 agttcttttt ccagctggta ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc
     8281 tcttttttta ttactatttg tttttttgtc aaaacaaata gcaacctgtg atttttccaa
     8341 ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc ttggagaaat agaaagagtt
     8401 ccattcgtta ctgtaatacc tgaaaaacca aatataactg ctagccatag tgctcctaat
     8461 gaagcagcat gaattccgtc atctgatgaa tgcggatctg gtcctaaatc aatcaggcac
     8521 gcttcttgga ataaatgata cgcccattcc atttcttgga tacggcaagc ctctatggcg
     8581 tgaatcgctt tactcaacga tgagtcgtgg attgttttgt tttcatagta atgcaaattc
     8641 ttagaaacga ttggttgaga aaaatactca ggtaataagt ataacagcat gacaagatct
     8701 gcttgcttgc tgacttggag ttcatttact tcgctacggg cataatcaag taaaatagac
     8761 tggctgccag gacgttcttt gtaagtggtc aaatcaataa ctggcttact gagatacgtg
     8821 tcatcttgcg gaatcagctg ttctttgtta ggcactggca aatataactt atctaaaaaa
     8881 ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat atttaacagc taaggccaca
     8941 ttataatgcg ccagatagtt cgtatatgca ttattgtcga tatattctgt gtattcatct
     9001 ggaccaatga ctctgtgtat ctctaaatga tcatcgactt ttacagctct gctcagccaa
     9061 aaagctgctg tttcatgaat gagcgttttt ccctttgttt ctaaaaacga ttgatcctct
     9121 gttgcttgga catactcttg aacggcataa gcaaggtctg cactgatatg atgttctgcg
     9181 gcagctgaag caacttcttg tcttgttccg gttttgatat tcaatgcagc aaatttgggt
     9241 gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa gtgcaccttg atagtgataa
     9301 tctcttgctt tttgccgtgc gccttctaat cgatcataac ggtacagcag caaattcttt
     9361 gctacttcag gaaactgata taaaaagaaa ggcaggataa aaatttccgt atcccagaaa
     9421 acatgccctt tataaccttc gccagttagt cccttcgccc ctacactaac acttggatca
     9481 ttgcggggag tcataatctg taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa
     9541 gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa aatctgtcca ataagacatc
     9601 gactgctcta aaatttctgt ataactaacg cttttctggg cttgggcaat acttttctta
     9661 aacgggtctg tttgctgaag agaggtagag agcacgatga tcttctccag cgtaaaggac
     9721 gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat
     9781 acacctgctt tattgaattt agctgcaatc ccaatatcaa ttttactttc tgttgtctgg
     9841 taggatgcac tgatcaaatc gtcaccaaat actcgcagtt ctttttcaac caactgctgt
     9901 gtaccaaaat tggtttgctg tccatcgata cccgttttca catctacttg gcagtcttcg
     9961 gtcaacggct tgatgaccac tttcagggct gcctgatgga gattattttg atcagcaaca
    10021 cgacaagtaa tgacttccac tttttttcct tgagcagttt caatgagcgc atagcgttta
    10081 agctctccca tttctaaatc tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga
    10141 gaaaaatgtt caccgtcgat ttttatttcc catctgatga catctgggag attcactaac
    10201 tcggctgatt ctgctcctaa aggtcggtta tatacaccag ctgcaaacat tccccttact
    10261 tgattccgat agtcttcctc ctggcttcct ctgataccaa aaaatccatt tccaatggtc
    10321 atctgactcg catacttatt gatatactct aaatcaaact gtttttcttc tactttcata
    10381 aggtaataac ttctcctgtt tctgccgaac gatataaatt ttccaccaga ttctggatga
    10441 catatccttg ccagccatct gcaatcatct gagtctggtc accgagcgcc cgattcacaa
    10501 aatgagccat acttagttca tgtgggtcat taactggcgt ggtcttttgt aaggaaacca
    10561 gctttccatc tacatcttcg aatagttcgc ctggatacag attagcacct gcctgatcgc
    10621 cacaaaaagc cacattcagt ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt
    10681 ggatgatctt gccgttttct aattccagtg tgccaaaaat tgcatcttct actgtgaatt
    10741 tgcttggatc ccatttcccg aatttccctg aatttttttt cgtaccaatt ttttggaagc
    10801 tgtacgcccg aacgcttttt actttaggaa atccagtaat atacatcgct gtatcaagca
    10861 tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc
    10921 cccagcctgg aacaccagac cgacgaagcg cattcgcttc tacgtaataa atatccccga
    10981 gaacatcaag atgattcttg atcattgttg cttcgtcaga aaaacgatgc tggaatccat
    11041 aagccaaaat ctgcccgttt ttatcggctg tctctgccat ctttcttgct tgatcagctg
    11101 tcatagctgg tggtttctcg cacatcactg aagcattagc ttcgagggca gccaatacat
    11161 gttcgaagtg aaagcgattg ggtgtacaga cactcacgat atcgggagaa atctcattca
    11221 gcatttgtgt aactgatgta tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg
    11281 ctcgctggct gactggatca accacagcgg ccaattctaa gacatctgat cttttttgat
    11341 aataagcagc atgtactttt tctgctactt gtccagcgcc tatgattgct actttcttca
    11401 tttatttctc tctcctttgc ttatttactt attgttattc tttctctatt ctttctctaa
    11461 atcatttaaa tgacgcgctg tttccagaca gtttttcaag tattgcaatg attcctgata
    11521 aattttcact tcattttctc ctcgcaaacg acattcatat accatataac cttcatactc
    11581 attttgaagt agcgtttgaa aatggcgagt aaaatcaatt gcgccagatc ctggttggta
    11641 gcgatgatta tctgctaaat ggatatgacc cacatactct ttttgcgtag tcaatgcttt
    11701 tgaaatatca tcttcttcaa tattcatatg ataaaaatca gcgatgatct gcacatgttt
    11761 caattgatta gagtcaatgt aatgacgcgc atccgctaac aaattgatca tatggtcttg
    11821 gtatcggttc aaaggctcca aataaataac cgtacctgtt tcttcggcta ctttatctaa
    11881 ataacgaagt gattcgctga ctgctttata gtctccttct ttgcttcggg gtgaaaccat
    11941 cggcggtaat cgataagtga acattcccca agctgctggg ataacgattc ctttgcctcc
    12001 aacctcgctt agtgcacgca aaatctcagc aatttgacgt acaccattaa gacgccgttc
    12061 ttcgataaag tcaccgatcc acccatcgta gccgttacaa gctgtcacta ctgggatccc
    12121 tgtttctttg attgcctgtt tgactgtgtc gatattttct attaacaact taccgtcgat
    12181 ttcgtattct tcaaatccac agtctttgat aaatctaaat ttttctaaaa tgtctcttgg
    12241 gaaaaatgct tgatcttgcg taccgaattt cattgttctc ctcctcggac ttgaacgccc
    12301 attttgatac tttcttctgg gtgctggtct acataacgca tgtaagcttc tgcgctttct
    12361 gcaaatggca caactggatc aatgatttct tcacaatcca agtaaccgtt catcaataat
    12421 tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg ggtaatctgg gtttggttca
    12481 ctagatgctc gagcaaagac gatttttgca ttattgaaat gtgcttcgcg accaagatta
    12541 aacccagctg gaaaaggttt agcaaaggca acataagaaa tgatcccgcc atagccgatc
    12601 cctattaaag aagcttgtaa tgcggcagca ctgccactcg tttcaataac tgaatctact
    12661 ccacgtttat ctgtcaattc ctttagacga aaaccgacat ctgctttcat aggatctagc
    12721 gtgtaatcgg caccgtgttt ttctgctaat tctctacgct gcgctatggg atcgatccca
    12781 acgatggtag tagcacctgc ttttttagct aattgaatcg caatttgccc gatagcgcct
    12841 aaaccgaaga ttgctacagc atcacctgct cgaacatgtg cttcccggat accacctaaa
    12901 gcgaattgag caggatcgta acatactgca tttttccatt gatccaaccg atcaagttta
    12961 cgtagtttat aattatcaac accgtttgta atcagcgttt cacgaatggg tccataagtg
    13021 cagactaact ctcctttttg atactctgtc accaaactgc ccgtttcttc gatcgtaccg
    13081 acaatcatat ttcctaattg aaattcgcca aataccacac cgcgttggct tccttcttct
    13141 cttggcaaga ataggcgcca ttcttctgaa aattcttcat cgataaatgg acttaatcct
    13201 ctaaagtcga caatttctgt tcctggtttt ggagtagcga aaattactcg gatacgtact
    13261 tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc cggctattct tggttctaat
    13321 gcaactaatt ttttcatctt ttctttcctc tttcttttct ttttatcctt ttacgccgcc
    13381 ttcgactaaa tcacctttaa taaatcgttc agataacgca tacataatga tgactggtaa
    13441 agcggtcacc aatgaagcgg ccatcattcg tccccaaatg tagtctggtg tactgaataa
    13501 tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa
    13561 taaataatca ttccacgaaa ccataaagca ataaacgaag acagaaatga ttccagaaat
    13621 agccaatggt ataatgatcc gaataatgat ctggatccgg ctaagaccat cgatcattgc
    13681 tgcttcttca atatcagtcg gaatcgtgtc aaaataactt tttagcatga atatcgcagt
    13741 aggaagtgtt tgaacgacca tcgtgataat cagtgctgta ttggtatcat acaaacctag
    13801 accggaaatg attttaaata atggaacaac gatcaaaata ccggaaaaca tataaacggt
    13861 gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc
    13921 cccgctgatc ccaatcacta ctgcagttac ggaggctatc aaggagatca ctaaactatt
    13981 tttgaaatac atgatgaatg gaaaaatctc agcattgaaa atatcctgat aatgttcaaa
    14041 agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa
    14101 tgatgtcatc agcatgatca aaaatggaaa caaattgaca gctaatagca acaaaatcgt
    14161 cgcgtaaaat aagattttcc tgctcttttt atttttcatt tcaagtccac cttctttcgt
    14221 gagagcaaga cgaccgctgc aataatgatg aacaatagaa tcgatatagc tgctgctttt
    14281 ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa cgcctaagat attgacttta
    14341 ttcgtcagta gatagacatc tgtaaacata tagaacatcc agatagatcg taaaacaatc
    14401 acagttgcta gtactggttt gattgccggc agtgtcacga tcttgaactt ctgccaagca
    14461 tttgcaccgt ccatttcagc tgcttcataa agcgttttgt cgactgtttg caaaatagcc
    14521 aaaaatgaaa taaacgcata cggatagtat ctccagatag caaacaaaac gaccataaag
    14581 aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt tgaaaagctg caacctatct
    14641 actaacagat cattgacgat tccataacta ttattgaaca tatatttcca agcaaaaatc
    14701 aaagaaatag atggcgtaac ataacttaat aaaatcagtg aacgcaccgt tttcctaaat
    14761 ctgaacttgc ggttaagcaa aatagctacg gctaatccta aagctgtact gcctgctaca
    14821 actaagaatg tgtaagcaac tgttaaacct aaagaacgat aaaattcact atcacttaaa
    14881 atttcaagat aattgcttaa tccgataaaa acggcgtctg agttaggatt cagcggcata
    14941 tcgaaaaaac tgatctgaat attggaaagc attggaatca gcactaaaac agctaataaa
    15001 agcatgcttg gtgctaaaag tcccatggct aattttaagt cagaatgttt ccttggacga
    15061 ttcattgaaa cctccccctg ataatctgcc aatagctatc tacttttttc gaacaccatt
    15121 ggcagaactt cttgttattt cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct
    15181 tcgtttccga cagttacttg gttcactgct tttccgatca cacctgagct tgtgatatct
    15241 cccattttcg tgaaattctt ttccccgact agtccaaaga cttgtacttc atcaaaagca
    15301 ctagcaatct cagatggcag ctcgccaaaa gacttgatga cttcattttc tttgtaacca
    15361 tccagttcga ctacttgttt attcactggt tgagcaccac caggagacat caagacccac
    15421 ttttccatgt ttttcggttg agataagaaa gctgtgaatg cttcagcagc ctctttttgt
    15481 ttctcgtcta gaccagaaga aatcgtcaat ccagatactg tgccatagac agcttgatct
    15541 ttttgtgttg ggatagcaaa accgatattt tctgagttcc ccgcttcata aacagaaggc
    15601 aaaatgtaag ttgaatagat tgccattggt gctgttccat tcatgaaagc atctttgatt
    15661 tctgtcacat cattagagcc tggcatcgtg tattgggata agttttgata aaacgacaat
    15721 gcttctctca tcttttccgt atcgatcgtt acctcgcctt catcatccaa aacatttgct
    15781 ccatttgaca aagcaaattg agagaacgct tgctcactca ttgttccttc agcagtaggc
    15841 atagcaattc catattgttt attctcttta tctgtgaaat gttgagcaat ttttaaaata
    15901 tcatcccaat tttctggttc tgaaaaacca gcttctgata atttttcttt gtcataccaa
    15961 ataccttgaa cccagccgct aattggtgca gcaatatagc tttttccatc ttctgaacga
    16021 acaaggtttt tggctccgtc ataataatta tcttctccag cttcctcgat aacgttttgg
    16081 actgccgtct gatcaatcag ttcatcttta tccatcactt tagcaaaatc ctgactgact
    16141 tccattactt ctggtaattt accggaacga gctaacgtaa cgatttttgt attgaacgct
    16201 gattcttcta ccggcacttg cttgattttg atgtctggat tttctttttc aaaatcagca
    16261 accagttcat tgatcacact taatcgttcc tgttcgacag aagagtgcat gaattgaatc
    16321 gttgtctgtt gtgattcgtt agagctttga ctttcaccac aagcagtaag aagaaatcca
    16381 gtagctagca gacaggtagt aacaataatt tttttcattt ttttccacct cttattttat
    16441 tattttcagc cacatatatt catacggctg gaatatcaac tttcgttctt gactgcaatg
    16501 gataatttgt ccagaaaaca ggtctaggta atctcctttt ggtaaaagat aacctgctga
    16561 ttgattggaa aaattatgga taactaagac tttctcttcc ttcgcttcgc gaataaaact
    16621 gataaaagaa tctgctgagt ccagtaagta aatcaaacta ttcggatgga aggctttttc
    16681 tttttttcga atagatagca tctgggtcaa ttcaccatat actgctgttc ttaatggatc
    16741 tgtctgcaac tctttctcaa tatctgctaa caaaaatttt tgccgattga tttttcgatt
    16801 ctctcctgtt ttttctacgc ctttgtaatc atttcgactt cccagcacac tttgaatata
    16861 aattgccggc acgcctggta acgttaccaa aaatgaatga gcagctaaaa agcgtttgct
    16921 tctccattcc gtcgtatctg ttggagaact taacgcatct agatacgtag cgtttatctc
    16981 atatggactg gtcgttccat ctgggttttt cttgtagttt actcgggcac cctctttctt
    17041 cagctgttcc actaattcaa ggatcgtttt ttccggcaga atacctcgta ccggattcaa
    17101 tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc
    17161 gatggttttt aaccattctt tcaacttact tgtattaccc gaacgaatcg cttgaagggt
    17221 caaaggtggt agcgaaaact gatagaccat ctgtgcttca ttctcgccat tcccaaaata
    17281 actgatatta tctttgtgtg gcacatttgt ctcagtgatc aataccgttc ctggagctgc
    17341 ttcatcagcg attgcacgga ataattggat gattttatgc gtttctggca ggtgtataca
    17401 agaagttcct agttctttcc ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc
    17461 tttttctaag taaaacaaaa gaatatcgat cattttcaat aaaacttctg gatttttaaa
    17521 attcaagtct acttgatctt cgctaaaagt cgtccatacg tgtttttctt tcccgttttt
    17581 gtcaatgaac gttgtaagta acgggctggt ccttggtcta gtcacgctgc ttgtatctgt
    17641 atccagtggc aaatcaataa aaaaatcttt gtacgtatca ttcaagttta aatattcctg
    17701 aaaccaagta ctttcggcag acatatgatt gcagacaaag tcgaacatta accgtgtttc
    17761 gcttctcatt tcttctatat cttcccatgt gcccaaacgg tcgttgattg cctgataatt
    17821 tacaacggag aaaccatcat cagatgtata tgggaaaaat ggcaagaaat gtacaactct
    17881 aaatgtatct gccaaatact tttgataaaa tttcttaaat gtacgcaaag ttggttgctg
    17941 ctcttcaaaa aattgatccc cataagtaat caagaaaata tcttcttcgt cccaatgcag
    18001 tttgcctgcc tggcctttct tctgctgata aacactgact ttttgttcaa tcatagaggc
    18061 ggttttctca gacaaatctg agtcgcgata aatcgcttgt aaaagttttg tatacatcgg
    18121 attccccttt cttttttggg agcgttccca acgatttgat catattatgg aaaacgctta
    18181 ctgtcaatag cattttttta aataattttg atatacacaa aaatacaaat aagaaagctt
    18241 atgtatcaat aattaatagc ttcttaaaaa aatatggtct ctcatgatca tgcaagctat
    18301 tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg aattattcta
    18361 aaacgctccc aaaaaggagg tgcatataaa attgaaacct actatttatg acattgcgaa
    18421 aaaagctaat gtctcaaaat caactgtctc cagagtatta aataaccagc caaatatctc
    18481 tgataaagca aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa
    18541 aattgcaaga ggactgtcag gaaatggttt tgacgcgatt ctagcattat ctcatcgtac
    18601 ttctcatacg acctatggca atcctttttt ttcagatgtt attcaatcca tttctgccgt
    18661 ttctgaacaa aataacttcg acttgattct acaaacggca aaaacaccag aagaagagct
    18721 agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga ggaattatta ttttaagctc
    18781 tacgatggat gaaacacttc tttttgaatt ggatcaatat aatattccta ttgtggtaat
    18841 cggaaaagtc ggaaacgact acacgaacat ctactcagtg gacacggata atttccagga
    18901 tagttacgat ttggtggaaa cactgatcaa gaatggacac cgtcatatcg gctgtctcca
    18961 tgctccgaca gaagtttatg tatccaatga tcgtctcaat ggttaccgtc aatgcttatt
    19021 tgatcaccac ctagatatcc gtaatgaatg gattatagac ggcggctttc gcttagaaga
    19081 tagcatggca gcaatgaatg atttaatgaa gctacgagaa agaccaactg cgattttttc
    19141 taccgatgca ttaaaatcat tgagtattta caaagtggca gaacaatctg gaaagcagat
    19201 acctgatgat ctttcgatcg caggattcag tgatccctta ttgtcctccc tattttcgcc
    19261 acagttgact cggatagaca ttccgacaaa tcagttagga atcaaagcca cagaactttt
    19321 attccaacta atccaccacc aataccctga agaaaaaaga atcattcttt caaccgagat
    19381 tactctcaca gattcgatcc aacaacaaga aatgtagaac agaattataa aagaacagaa
    19441 acactgagga taagtcagtt actctgttct tttttcaatc actcatccct cgttaccccc
    19501 tcccggtatt ttagcgaatt tttcttattt tccatctgtt ttagatagct gtggataact
    19561 ataatgtttg tgttaaacta ttttttccac agggaagaaa aaactatttt taaccacgtt
    19621 gtgtataagt ttttatagtt cgttttttta aacacattta cccttatttc aatccatgtt
    19681 atttggggat acttatctct aaaacataaa tagaataaca gacatgtcta ctggttttgt
    19741 tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc gctctatctt
    19801 cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg attgacttct
    19861 cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt taagatcctt
    19921 tcccactttt cttctagtta gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca
    19981 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
    20041 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
    20101 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
    20161 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
    20221 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
    20281 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
    20341 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
    20401 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
    20461 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
    20521 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
    20581 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
    20641 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
    20701 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
    20761 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
    20821 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
    20881 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
    20941 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
    21001 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
    21061 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
    21121 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
    21181 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
    21241 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
    21301 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
    21361 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
    21421 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
    21481 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
    21541 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
    21601 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
    21661 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
    21721 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
    21781 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
    21841 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
    21901 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
    21961 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
    22021 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
    22081 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
    22141 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa
    22201 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
    22261 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
    22321 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
    22381 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
    22441 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
    22501 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
    22561 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
    22621 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
    22681 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
    22741 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
    22801 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
    22861 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
    22921 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
    22981 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
    23041 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
    23101 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
    23161 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
    23221 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
    23281 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
    23341 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
    23401 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
    23461 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
    23521 cacagaacaa cccttcacgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
    23581 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
    23641 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
    23701 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
    23761 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
    23821 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
    23881 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
    23941 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
    24001 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
    24061 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
    24121 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
    24181 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
    24241 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
    24301 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
    24361 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
    24421 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
    24481 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
    24541 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
    24601 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
    24661 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
    24721 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
    24781 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
    24841 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
    24901 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
    24961 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
    25021 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
    25081 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
    25141 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
    25201 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
    25261 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
    25321 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
    25381 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
    25441 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
    25501 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
    25561 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
    25621 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
    25681 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
    25741 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
    25801 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
    25861 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
    25921 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
    25981 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
    26041 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
    26101 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
    26161 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
    26221 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
    26281 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
    26341 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
    26401 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
    26461 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
    26521 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
    26581 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
    26641 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
    26701 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
    26761 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
    26821 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
    26881 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
    26941 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
    27001 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
    27061 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
    27121 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
    27181 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
    27241 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
    27301 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
    27361 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
    27421 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
    27481 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
    27541 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
    27601 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
    27661 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
    27721 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
    27781 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
    27841 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
    27901 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
    27961 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
    28021 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
    28081 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
    28141 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
    28201 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
    28261 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
    28321 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
    28381 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
    28441 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
    28501 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
    28561 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
    28621 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
    28681 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
    28741 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
    28801 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
    28861 acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca
    28921 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
    28981 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
    29041 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
    29101 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
    29161 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
    29221 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
    29281 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
    29341 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
    29401 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
    29461 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
    29521 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
    29581 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
    29641 gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca acggctttgc
    29701 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
    29761 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
    29821 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
    29881 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
    29941 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    30001 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    30061 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    30121 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    30181 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    30241 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    30301 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    30361 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    30421 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    30481 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    30541 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    30601 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    30661 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    30721 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    30781 taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct
    30841 tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc
    30901 ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta
    30961 cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg
    31021 gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt
    31081 tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa
    31141 atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct
    31201 gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta
    31261 tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa
    31321 cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg
    31381 cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt
    31441 cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg
    31501 attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg
    31561 atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc
    31621 atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc
    31681 tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa
    31741 aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt
    31801 cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg
    31861 agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc
    31921 acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct
    31981 ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct
    32041 ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt
    32101 tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg
    32161 taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag
    32221 tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag
    32281 cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct
    32341 cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat
    32401 cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg
    32461 cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc
    32521 tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat
    32581 ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat
    32641 cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc
    32701 gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc
    32761 ctttttattt atcctactta ctttttccta attctaggat acctacttat atgttactac
    32821 acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa
    32881 aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat
    32941 tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata
    33001 ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag
    33061 tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat
    33121 acattcctaa agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag
    33181 gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag
    33241 acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg
    33301 aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt
    33361 caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg
    33421 tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag
    33481 atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta
    33541 aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg
    33601 cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt
    33661 ttttattggt aaatatcgat gcagaagtgt taacgatgaa tacgccactt caaatcatta
    33721 atccaaatga tcaagtagtt gtatttaatg tcatcaacag ttcttctgca ttgaacgtac
    33781 agtcaccaat caaatacaat gatcgttcaa atcatgaaac agaagatttt tcggatgcga
    33841 atatctcttg gaattttgga aatgagatga ctgatttgac aatcagtgct ccttttcaag
    33901 gaacaatcct tgcgcctaat gctactatcc gcattactca aaaccaagac ggaacgatta
    33961 taggaacaaa tgtgatttta gatgcagcta ccaatcgttg ggatcctaac gaaatattta
    34021 tcagcgacaa tggtacagat acgacagaca caagcgacag tacagataca tctaccacaa
    34081 gtgatagctc agattcatca
//
LOCUS       DASJOX010000019        31740 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJOX010000019 DASJOX010000000
VERSION     DASJOX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.019"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..224)
                     /locus_tag="U4A12_000852"
     CDS             complement(27..224)
                     /locus_tag="U4A12_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1773260.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(618..2345)
                     /locus_tag="U4A12_000853"
     CDS             complement(618..2345)
                     /locus_tag="U4A12_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN1773261.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2338..3531)
                     /locus_tag="U4A12_000854"
     CDS             complement(2338..3531)
                     /locus_tag="U4A12_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1773262.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3718..4362)
                     /locus_tag="U4A12_000855"
     CDS             complement(3718..4362)
                     /locus_tag="U4A12_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1773263.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4456..4590)
                     /locus_tag="U4A12_000856"
     CDS             complement(4456..4590)
                     /locus_tag="U4A12_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN1773264.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4592..6724)
                     /gene="feoB"
                     /locus_tag="U4A12_000857"
     CDS             complement(4592..6724)
                     /gene="feoB"
                     /locus_tag="U4A12_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN1773265.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6721..7194)
                     /locus_tag="U4A12_000858"
     CDS             complement(6721..7194)
                     /locus_tag="U4A12_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN1773266.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7595..7819
                     /gene="nrdH"
                     /locus_tag="U4A12_000859"
     CDS             7595..7819
                     /gene="nrdH"
                     /locus_tag="U4A12_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN1773267.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7978..10137
                     /gene="nrdE"
                     /locus_tag="U4A12_000860"
     CDS             7978..10137
                     /gene="nrdE"
                     /locus_tag="U4A12_000860"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN1773268.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10187..11152
                     /gene="nrdF"
                     /locus_tag="U4A12_000861"
     CDS             10187..11152
                     /gene="nrdF"
                     /locus_tag="U4A12_000861"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN1773269.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11241..12380)
                     /locus_tag="U4A12_000862"
     CDS             complement(11241..12380)
                     /locus_tag="U4A12_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1773270.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12689..13402)
                     /locus_tag="U4A12_000863"
     CDS             complement(12689..13402)
                     /locus_tag="U4A12_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN1773271.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13656..14357)
                     /gene="nagB"
                     /locus_tag="U4A12_000864"
     CDS             complement(13656..14357)
                     /gene="nagB"
                     /locus_tag="U4A12_000864"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN1773272.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14591..16123
                     /locus_tag="U4A12_000865"
     CDS             14591..16123
                     /locus_tag="U4A12_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1773273.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16314..17516)
                     /locus_tag="U4A12_000866"
     CDS             complement(16314..17516)
                     /locus_tag="U4A12_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1773274.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17598..18287)
                     /locus_tag="U4A12_000867"
     CDS             complement(17598..18287)
                     /locus_tag="U4A12_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1773275.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18393..18761)
                     /gene="rplL"
                     /locus_tag="U4A12_000868"
     CDS             complement(18393..18761)
                     /gene="rplL"
                     /locus_tag="U4A12_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN1773276.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18814..19317)
                     /locus_tag="U4A12_000869"
     CDS             complement(18814..19317)
                     /locus_tag="U4A12_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN1773277.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19521..19648)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19727..20416)
                     /gene="rplA"
                     /locus_tag="U4A12_000870"
     CDS             complement(19727..20416)
                     /gene="rplA"
                     /locus_tag="U4A12_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN1773278.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20520..20942)
                     /gene="rplK"
                     /locus_tag="U4A12_000871"
     CDS             complement(20520..20942)
                     /gene="rplK"
                     /locus_tag="U4A12_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN1773279.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21285..21953
                     /gene="sdaAB"
                     /locus_tag="U4A12_000872"
     CDS             21285..21953
                     /gene="sdaAB"
                     /locus_tag="U4A12_000872"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN1773280.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21966..22838
                     /gene="sdaAA"
                     /locus_tag="U4A12_000873"
     CDS             21966..22838
                     /gene="sdaAA"
                     /locus_tag="U4A12_000873"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN1773281.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22929..23948)
                     /locus_tag="U4A12_000874"
     CDS             complement(22929..23948)
                     /locus_tag="U4A12_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1773282.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24121..25026)
                     /locus_tag="U4A12_000875"
     CDS             complement(24121..25026)
                     /locus_tag="U4A12_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1773283.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25085..25630)
                     /gene="nusG"
                     /locus_tag="U4A12_000876"
     CDS             complement(25085..25630)
                     /gene="nusG"
                     /locus_tag="U4A12_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN1773284.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25731..25901)
                     /gene="secE"
                     /locus_tag="U4A12_000877"
     CDS             complement(25731..25901)
                     /gene="secE"
                     /locus_tag="U4A12_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN1773285.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25925..26077)
                     /gene="rpmG"
                     /locus_tag="U4A12_000878"
     CDS             complement(25925..26077)
                     /gene="rpmG"
                     /locus_tag="U4A12_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1773286.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26239..26883
                     /locus_tag="U4A12_000879"
     CDS             26239..26883
                     /locus_tag="U4A12_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1773287.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26939..27442)
                     /locus_tag="U4A12_000880"
     CDS             complement(26939..27442)
                     /locus_tag="U4A12_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1773288.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27455..27501)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28039..29004
                     /locus_tag="U4A12_000881"
     CDS             28039..29004
                     /locus_tag="U4A12_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1773289.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29103..30275)
                     /locus_tag="U4A12_000882"
     CDS             complement(29103..30275)
                     /locus_tag="U4A12_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773290.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30262..30387)
                     /locus_tag="U4A12_000883"
                     /pseudo
     CDS             complement(30262..30387)
                     /locus_tag="U4A12_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            30495..31574
                     /locus_tag="U4A12_000884"
     CDS             30495..31574
                     /locus_tag="U4A12_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1773291.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         9674 a         6569 c         5715 g         9782 t
ORIGIN      
        1 caatttgctt agcatttttt ttcattttag cacttccctg gaattgctcc acctaaaaaa
       61 ccacctatag acattcctgc aatacaagta gttgccttgg cccaatcgac cgtacattta
      121 tttttagtgc aatacactcc atttccataa tattttccac tatgagtggt acccccatag
      181 ataagttgtg tctctttaat agacaaaatt tttaaatgtt tcataatcta atcaccccta
      241 aattaatgtt tttagactat tattctaaca ttcattaaat ttcaaaaaac aattccttct
      301 tgaaacgtag caaaataatt cctgaataag aatttaatca cttttaagta tataaactat
      361 gtatattctt taatattttt tgagaatgca cttcgtacat aataaacttt aatcacatta
      421 gtctttcata cctacgatcg atctctttga ctcgcaccca taaattcata atagtttgtc
      481 ggcgtctttg gacgtttcga agatgagcta cgcattctct ttttttatag acaaaaaaga
      541 tagagacgaa catggagctt ccaaatttgg atatttgatc caattttgga agctccattt
      601 atttcgtgtc tgtcttttta ttttttccag ctttcgacta attcttggtt ctctttgatc
      661 cagtcttttg ctgcttgctg tggatctttt ccattgttga tttccaacat aactttttcc
      721 atatcttttt ctgaccaatg gaaattgtct aatactttgt aagcttctgg ctggtcttct
      781 ttcaatcctt ttcgaaccat cgtatggatc tgctcttcgc ttcccattcc gttttcagga
      841 tcttctaagt atttcagatc atatttggca aacatccaat gaggtgtcca tcctgtaact
      901 acaattggtt cgtgtttttt gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa
      961 gatgtctcta ctttccagtc tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc
     1021 gtaactacac cagctcctgg ctcgattcct atgattttct tacctgcctg atcggttaac
     1081 tcatcgatcg aatttacatc catgtaggct ggcactgcta aaccaacttt tgctcctgtc
     1141 aaattggcac ctaggtcttc gacttgatct ttatattgtg catattgtga accatgagtt
     1201 ttaggtagcc aagctgaaac catggcatct gactcgccat ttgaaacaga tttccacatg
     1261 attgaattat ctaatggcgt catcgttaca tcatagccca tttgtttcaa tacttcaccg
     1321 actacatttg ttgaagcaac ttctgtatcc cattcaacat aagataagtt gatacgtttt
     1381 gtttctttgg cagatgaaaa actccctata ccaatcgcac ctaagatcac tactactaca
     1441 gcaccaatga tcagtccttg acgtttttta cttttctgtg ttgttgctcc tgcagctttt
     1501 ttattgtttg gctggttcaa atgctgtgta aaacggtcaa tgatgattgc taagataact
     1561 aatgcaaccc cgttgacaaa tccattcccg acttgtgctc tttgcaatgc tgataatacg
     1621 ccgcgtccta agccgggtgc accgatcata gaggcaataa cgaccattga aagtgacagc
     1681 attgttgttt gattgatccc tgccatgatc gtacttttag ctaaaggcaa ttctagtttg
     1741 aatagttttt gacgacttgt gctcccgaat gaatcagaag cttctactaa ttctttagga
     1801 acttgtcgaa tccctaaatt cgtaaaacga acggttggtg gcagagcaaa gatgactgag
     1861 gcaaataccc ctggaaccat cccgataccg aaaaatgcaa ctgctggaat caaataaacg
     1921 aatcctggca ttgtctgcat aaagtcaagg atcggtttga taatactttg ggctttactg
     1981 ctttttgcca tcaagatccc taatggtacc ccaatgatga tcgagatcac actagataaa
     2041 agaacaagtg tgacagtatt catcaaatca ttccatagac cttggttata aataaaccat
     2101 agaccgatta atgtgaacaa cgtaagtcct ggacgtttct tagaaataaa gaaagctgct
     2161 accgtcaata acaagataaa caaaggagct ggaatcacta acagtaaatt tgtaatcccc
     2221 gacatcactg cctgtccaat cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta
     2281 aaccattctg tgatattttc tacccagttt gccactggta attgatagtt caatagatta
     2341 ttcatgttca ctcacctcgc ttgtttctgc taatgcttca atcacactac ctcggatgac
     2401 gacacctttt aattttccat cctctacaac tgcaagtgga gtcggtgagt cataaataat
     2461 attgaaaata tcacttacca ccatgtcttt cccaatcacc gtcacatttt catctaggaa
     2521 gtcgatcaat ggacgttttt gcttcctagc ttctaacgct tgatctgcag tgatgatccc
     2581 tttcaggtgt ctctttctgt caacagccat cagcatgctg acttcttcat tacgcatcct
     2641 tgtcaatgca acgtttggtc catcagattc gatattcgtc gtcaatgcag gtaccatgat
     2701 gttttgagcc gtaagcactt tggaacgatc gacttcttcc acaaattcac gtacataatc
     2761 atttgctgga ttggtcaata tttcttcacc tgttccgatc tgcatgattt cgccgtcttt
     2821 catcaaggcg attctgtccc caatccgtaa agcttcattc aaatcatggg taatgaaaat
     2881 aatcgttttt tgaacattgg cttgcaggtc cagtaattcg tcttgcattt ctcgccggat
     2941 cagcggatca agagccgaga acgcttcgtc catcagcaag atctcaggat catttgctaa
     3001 agctctggct aggccaaccc gttgttgcat cccaccagat aactgactag gatattggtc
     3061 tttaaatgaa agcaaactag aattttctaa tgctttttct gctttttctt gtcgttcttc
     3121 tttaggtaca ccacggactt ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg
     3181 aaacagaccg aagttttgga aaaccatatt gattttatga cgacggactt ctcgcaattc
     3241 ttctttgctt aacttcgata catcttctcc atcaatatag atactgcccg aagttggctc
     3301 aatcagacga ttcaatagac ggatcaatgt ggatttccca ctcccggaaa gccccatgat
     3361 cacgaagatt tctccttctt ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc
     3421 ggttttcttt aaaatctccg ttttcggctg gtgatcattc atcatatcta aagcggcttg
     3481 tgtcttttta ccaaatatct ttgttaaatg ttttacttct acttttacca aacaaattct
     3541 ccttttttat gtaaattcat ttttctttct gaaaaaagtg accacactat gttaacatca
     3601 tgtagtcact ttgtcaaaaa tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct
     3661 aaatctttgt aaataaaagg tttagccaag ttaatatttt tttaatattt cacacaatca
     3721 ttctgcaagc tgatgctccg ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt
     3781 tccaataaaa tgaacgatgt cccctgccgt aaatttagca taggggccag gtgaaacaag
     3841 cagttcttca ttatgagaaa ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa
     3901 ctcgctaatt gaacggtcca gatgcattgc tttttctgat aaagataatt caaacggaag
     3961 taaaggattc atcttatgta ttcgcttcgt ttgattgacc agctgatcca ataattgaga
     4021 aaaatgattc agctcatctt gttgcttctc cacacttgcc atgatttctg cttttgtatc
     4081 ttggatggat tgtacatctt gatattgcaa taggaaatct tttgcttttt ccttcgaagc
     4141 aacaaatgcc ccacttccat gccgtacttc catgattcca agatctacca gcacattgat
     4201 agccttgcgg gtagtctcag gagaagcatt gtacgtactg gcaagtgttg agcgggcatg
     4261 gatcttctca cccactttat atcggttttc aacgatacgt tcggctaaat caacagcaat
     4321 ctgctggtag cgcgggcgga cgattttttt ctttccagac attttctttc ctctttcttt
     4381 ttttactttc cctcatctta ctatgctcta gcaaaataag caaaaaaggg gagaatagcc
     4441 tcttgcaacg actgatcatc gcttgatctg ttcctttttt actgggcaag ctgtttgaca
     4501 gtcatcacaa ttcttaccgg attttaaacg agtatagaca attgcgcctg cacttccaaa
     4561 gattaacaca cttaataaga atgtcgccat cctattcctc tccttctaat gacaatatag
     4621 tataaaactg gttcttggat tttggctgac ggaacaaaac ataaacgagt cctatcatca
     4681 gtaaaatagc aagatacgtc cctaacgcta cctgtccacc ctccaataag tgtcccaact
     4741 gataaatcac aaaactaact aaataagcta aaccgcattg atagcctatg gcgatccaag
     4801 tccatttctt ggcattcatt tcccgatgga tagccccaat tgccgcaaaa caaggcgcac
     4861 aaagtaaatt gaaggctaac aatgaataag ctgctgcggg agtatatgtg tgttgtagca
     4921 cttgccagat ttctctgcca ttttcggata cttccgcatg accaaacaaa ataccaaatg
     4981 tgccaataat attttcttta gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt
     5041 ttccccaacc aagcggctga aaaacaactg ccaggattcg cccgaaaaag gctaagatac
     5101 tttggtcttc agaaacaggt tgagcataaa aattataaga agagctgaac cagatcacga
     5161 tactagaaac aaagataatc gttcctgctt ttttaacaaa agacttgctg cggtcatagg
     5221 tttgtcttag gaccgctgac cattgaggaa aatgatatgc gggcaattcc atgataaaag
     5281 gagctgtatc tccagcaaac aaacgtgttt ttttcaagct gattccagag aaaataattg
     5341 cagtcatacc aagaaaatag gcagaaggag ctacccaaga ttgatgagga aaaaaggccc
     5401 ctgctactaa ggcaataatc ggcaactttg cagaacaagg catgaaggtt gtgaccatga
     5461 tcgtcaaacg acgatcttgc tcgttttcaa tcgtgcgact ggccatgacg cctggcactc
     5521 cacatcctgt agcaatcagc ataggaatga aggattttcc tgaaagaccg aatttcctga
     5581 aaagtcgatc catgacaaaa gcgatcctcg ccatatatcc gcagtcttcc agaaagctca
     5641 agcataaaaa caatataatc aattgcggta gaaatcctaa cactgctccc acgcctgcga
     5701 tgatgccgtt caagatcagc tcctgcatcc atcccgctac ttgccattca gctaacaatc
     5761 tgccaacatg ttctggtaac catgtaccaa acaatgtgtc attgatccaa tctgtcccca
     5821 ttgttcctac tgtttgaata gccagataat agatcagcca catcacaaat gcgaagatcg
     5881 gcaaggctaa ccaacgattc gttgcaatct gatcgatttt gtcactcatc gataattgga
     5941 aagaatcatt tgacacaaca cagagtgtac atagttgggt aataaattca taacgttcat
     6001 tgaccagaat cgtctcacta tcgtcgtcca atagtttctc tgttaactgg atcagttgct
     6061 cgatctcttt ttgctgagaa actgctatct cgtaatcctc tcttaccttc tgatctcgtt
     6121 caaaatattt cattgcatac cagcgtttct tgccgctttg ttctcctagt acatcttcaa
     6181 tttcatgtaa agctgcttct aaacgtggat catatgtaca gggaacagga atagaaggcc
     6241 gcttttttgc agactcctga atcttttcga ttagtttttc gattccttgc tttttcaata
     6301 cgctgattgg taaaactggt acaccaagac cataggaaag cttttctaga ttgatttttt
     6361 ttccttcttt tttgatgaca tccatcatat tcagaccaat gatcatagga atgtctaaat
     6421 ccaataactg cgtagttaaa taaagatttc gttctaaatt ggtagcatca ataatgttga
     6481 tgatcagttc aggctgttcc tgaaccaaat aatctcttgc aatcacttct tctggtgtat
     6541 aaggagacaa agaatagatg ccgggtaggt cctgaatatg aatgttcgga ttttttttca
     6601 acgatccttc ttttttttct actgttacgc ccggccaatt ccctacatat tgattcgaac
     6661 cagtcaagag attgaacaag ctagttttcc cgctgtttgg atttccgact aacgcgatat
     6721 tcattctttt tcctcccttt gtatcaagat tagttcggcc tctgactttc gtaacgtcag
     6781 ttcataccct cgcactgtca gttcgattgg atctcccaga ggggctagtt ttcggataac
     6841 tatttttaca tttcgcgtca atcccatatc cattaaccgt cgcttcactg cgccttgtcc
     6901 gtggattgct acgacgatcc cagtctctcc tgcttttaat tgatcaagag aagtccagct
     6961 ttcagaggaa gcagtctttt tattgacaaa gatttgacct agtatttccc gacttactgc
     7021 cactcgatga ctttttacca gtaaaatggc attgttgcct gataaattga cgacgacgat
     7081 tttacttccg gcaacgatcc cgatattttg caaatggcgt tttgtttttt cttctgtttc
     7141 gattcgttcg acttgataaa cgaaattctg ctttgcctgc gctaaggtct gcatattgat
     7201 tcctctcttg tgaaataaat catgtattaa aatcattcat tatatgttct aattttctta
     7261 cgtgttacgc aaataaatga gaatcattct cacatagaat actctcggta attgagaatg
     7321 tcaattatac ttgtgatatt atgagcgaat ctttagtatt gatagataaa aatgagaaaa
     7381 aaatatttct tcatagtttt ccacaatttt ttctctatta aatttgctat tttcccacac
     7441 gatacttgtt ttcagctctt ttttcaaaaa cacaaaactt agtattccac agtttccaca
     7501 acacaatatg tagtgtttgt tgtttgactt ttttactaag agttttatac tatctattat
     7561 agataataag aatgtgttag aggaggcact caccatgaac gtaaaaatct tttctaaaaa
     7621 taattgtatg caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga
     7681 agaagtcaat atcgatgaac agcctgaagc agtagactgg ttgaaagcac aaggattcca
     7741 aagtgttcct gtcattactt ctgatgcaga aacagttatc ggattccgtc ctgatcagtt
     7801 gcgtaaactt gccagctaac atgactaaaa gaatcaaaac gtgatacatg gatttgaagg
     7861 gcttgagaca gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac
     7921 aatgcaatga tttgcaatca agcatcatca aatacgaaat gaaggttggg cgtacgtatg
     7981 agtcttaaaa atttacaaga tgtcagttat ttcaagctga ataatgaaat caatcggcca
     8041 gtcaatggtc aaatcccttt gaataaggat aaagaagctt tagttgcctt ttttgaggaa
     8101 aatgtcaaac caaatacgat gacttttcta tctattatgg aaaaaatcaa ttacttgatc
     8161 gatcaagaat atatagaagc agaattcatt caattgtatt caccatcatt tattgaaaag
     8221 ctatatcaat ttttggacga gcaacatttt tctttcaaat cttttatggc tgcttacaaa
     8281 ttttattctc aatatgcgtt gaaaaccaac gacggttccg cttatctgga aacttatgaa
     8341 gatcgagtag ccttcaatgc gctttacttt gcgaacgggg atgaagaatt agcacttgct
     8401 cttgcagacg aaatgatcca tcaacgatat cagccagcta caccttcttt cttgaatgct
     8461 ggacgtaaac gtcggggcga attggtgtct tgtttcttga ctcaagtcac tgacgatatg
     8521 aactctatcg ggcgctcaat caactctgct ttgcaattgt cccgtattgg tggcggcgta
     8581 gggattactt tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct
     8641 gcaagtggtg tcgttccagt catgaaatta ttcgaagata gtttcagcta ttctaatcaa
     8701 ttaggtcagc gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc agacattatt
     8761 gctttccttt cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct
     8821 cttggcgtga tcgtacctga taaattttat gaattgacac gtaaaaacga agatatgtat
     8881 ttatttagtc catatagtgt cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt
     8941 acagctgagt atgatcatct agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg
     9001 cgtgatcttg aaaatgaaat ctcaaaatta caacaagaat ctggctatcc ttacgtaatc
     9061 aatattgata cagcgaaccg cgagaatcca atcgatggca agatcatcat gagtaattta
     9121 tgttcagaga ttttacaagt tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa
     9181 gtattaggaa cggatatctc atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa
     9241 agccctaact ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt tattacagat
     9301 gcatctgata tcgatgtcgt accaccgatc caaaacggaa ataagctgaa tcatacgatt
     9361 ggattaggtg ccatgggctt acacacttat cttgcaaaag aacaaatgga atatggctca
     9421 gaggattcat tagactttac aaatatttac ttcatgctgt tgaattattg gacacttgtt
     9481 gaaagcaaca atatcgctcg tgaaagaaaa caagttttcc ataattttga aaaatcagct
     9541 tatgctgacg gcacttattt cgaaaaatac gtaactggag aattccagcc aaaatcagaa
     9601 aaagtcaaag cattgtttga aggcattttt atcccaactg ctgaagattg gaatgctttg
     9661 aaacaagcag taatgaaaga tggactgtat catcaaaatc gcttggcagt agcaccaaat
     9721 ggctcaattt catatatcaa cgatacgagt gcaagtattc atcctatcac tcgcatgatc
     9781 gaagaacgcc aagagaagaa aatcggtaaa atttattacc cagcgcctta tctatccaat
     9841 gagacgattc cgtattacac ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt
     9901 tatgcaatgg cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt catgcgttct
     9961 gaaatcccag aaggcattta tgaatggaag acaacaacga agcaaacgac acgtgatttg
    10021 aatattttaa gacattacgc tttccacaaa ggaatcaaat ccatttatta cgtacgtacc
    10081 tttactgatg atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt gatctaatat
    10141 catagataga ttcgcttatt catattttaa ttaaaggagc attctcatgt cagaaacata
    10201 ttatgaagca ataaactgga atgaaattga agatatcatt gataaatcca cttgggagaa
    10261 gctgacagag caattttggc tagatacacg tatcccactt tcaaatgacc tagatgattg
    10321 gcgtacattg tctcaattgg aaaaagatac agtaggacat gtatttggcg gtttgacact
    10381 gctagatact gtccaatctg aaagtggcat ggatcagttg cgtaaagatg tccgcactcc
    10441 ccatgaagaa gccgttttga acaatattca attcatggaa tccgttcatg ccaaaagtta
    10501 ttcttctatt tttagtacac tgaatacgaa aaaagaaatc gaagaaattt ttgactggac
    10561 aaatacgaat ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg
    10621 tacaccgcta gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt tctattcagg
    10681 tttttatact cctctttatt atttaggaaa caacaaattg gctaacgtag cagagatcat
    10741 caaactaatc atccgagatg aatctgtaca tggaacctac atcggttaca aattccaatt
    10801 aggattcaac gagttacctg aagcagaaca agaaacgtta aaagactgga tgtacaatct
    10861 tctttatgag ctttacgaaa acgaagaacg ttatacagaa gaattatacg atccaatcgg
    10921 ctggacagaa gaagtcaaga cattcctacg ctataatgcc aacaaagcat tgatgaacct
    10981 agggatggat ccactctttc cagatactgc caatgatgtc aacccaatcg taatgaacgg
    11041 gatttcaaca ggcacaagca accatgactt cttctcacaa gtcggaaacg gctatttgtt
    11101 aggtcatgta gaagcgatga aagacgacga ttatttgatt ggattggaat aaggatttat
    11161 tttaattcaa aaatttttcg aaaaataaaa agagtttagc gtacagatat tttaaatgta
    11221 cactgaactc ttttttatgc ttattcactt gtttcttcca gcacttttgg actgtctaac
    11281 ttttccgaaa catgttccat tcttctcaat aaatagattt tccggatatt taatattatc
    11341 gtcttaatca ctgcatagac aggtatgcaa atcagcatgc ctaatattcc tgcgacactt
    11401 cctgctccaa tcaataccaa gatgatcgtc aaaggatgga tattcattga tttcccaaat
    11461 aacaacggct tgatcaaatt tccatctaat tgttggatca ctaaaatcga caacgccata
    11521 tacaaagctt gaaatggaga gataaacaaa ccgacaatga ttgctggtac cgctccaata
    11581 aaagggccga catacgggat aatattcgtg attccgcaaa tgaccgcaag caacaagcta
    11641 taaggctgat cgaatattct cattgccaaa aagctcatta caccaataat caaagcatct
    11701 aagatcgtac tgctgatata ggcagataat gtttcgttta attcttttac cgtttggcgt
    11761 aactctttac gaatcgcttt cggaaagaag tgagacaacg catcaataaa tttgtgccca
    11821 tccttgaaca taaatagcaa aataaatgga acggtaaata acaaaataaa aaattgcatc
    11881 ataaatgaaa tgatttttga gacacttgca gataaactga ctaatatgat attgaacaaa
    11941 ttactaagag ataaatttgc tgatgctaat tgttcttcca aattgaaatt tttaaactct
    12001 tcccgctgcg ccaattcatt taaccatttg cttgtttcat ctgcatacat tggcaagctc
    12061 tgggtcaatt gaattgtctg ttccattaac tgaggcacta cattcatgac agttaaaaca
    12121 actaaaatga ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg gatccgatgc
    12181 ttttcgagaa aaacaacgat tggatcgaac atataataga taaaaccagc aacaagcaat
    12241 ggtaaaaaaa ctgccgaaag cattttcaat attggactaa aaatattcgg catttgtaaa
    12301 ataaagtaca cgccgatgat cgtgagtact agctccactg tccagaaaaa taattttgat
    12361 tgtttaaaac gtgattgcat acatctcctc caaggtcctt tgatacttct attctatcat
    12421 ttaacaaaga gttacgcgct gagattttca tttgcttaaa aaaaacaaaa aaaataaaca
    12481 tattttaatg agtagacgag tacttaaaca aaaacacggt ttggagtagt gacaaaaatt
    12541 atactgcagt ccctttttcc aaataaacgg tgttcaaaag cttgcatctt cgtaataagc
    12601 ccagccaaac gaaaaatata aggaactatt tttatttggc tgggcttatt acttgatgta
    12661 ggacatctat tttacaacct cagatcattt attgcgcaat actgactaaa tgcaactttg
    12721 tgatattttt gtgtacggac aactcctcga tcacatcggc ataagtgagc ccttttggaa
    12781 gatcaatcgt atagatattt ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat
    12841 tcacatcttc gattacgata tttttctctt caaaataatc ggtaagaaaa tctttggttt
    12901 cttttctatg gataaattgg atttctaact ttttagtcgt aggtacatgg atcacgcgtt
    12961 tcactactgt gagtgcaata gcaatcccga taaaactgat gatcgcaatg gaatagtaac
    13021 ccatgccgat ggcgatccct aatccagcaa ttgcccataa agaagcggca gtcgtaagtc
    13081 ccgtcaccga ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg acaccactca
    13141 ccacttgagc aatcagcctt gcttcatctg aacgaatcgt tccagcaagc tgtggatact
    13201 ctaaagcatc tcttaatgca ccactagcga tctcaacttg gatcaatgca atgatacatg
    13261 ctcccataca aactaaaata tgcgtacgca tacctgctgg acgattttta tattgtcgtt
    13321 caaaaccgat tgctccgcca aatagcatag cgatccctaa acgtaaaata atctctggaa
    13381 ttgtcaatac catatcattc atctaaacac cctccagtcc aatatatcat gtctgaaagg
    13441 agcagacaaa aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga caaaattttt
    13501 gtcatagtcc tttttatgaa taaacggtgt tcaatcagct gaccctcggt attactcaac
    13561 aaacggcaaa atatggggag ctatttttcc tttgtcttct ctaatactca ggtcaaaacg
    13621 ctgatttcac aacctcttta gcctactatt gaaaattaca acttacttgc tgcttcttca
    13681 tcgatgatca caacaacatt gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt
    13741 acaggtcctt caatcatccc tttgattgca tcagctttgc tttcgccaaa tgccatcaag
    13801 acgatttctt tacttttcat aattgaaccg attcccattg aaacagcttt tgttggtaca
    13861 tcttctgctt tatcgaagaa tcgtttgttt gcattgattg ttgattctgt taagtcaact
    13921 actgaagtcg ttccgtcaaa tgaagcacca ggttcattga aaccgatatg tccattttgc
    13981 ccgattccta agatttggat atcgattgga tgtgcatcga tgatttgatc atactttttg
    14041 catgctgctt ccagatcttc tgcttttcca tctggtacaa acgtttcttt gaatggtttt
    14101 ttatcaaata attggacatc catgaaatga cggtagcttt gttcatctga accgcctagt
    14161 ccaacgtact catctaagtt tacagaagtc atttcactga aatccaaatc gcttgaagtc
    14221 atttctttat ataaagtaac tggtgtgctt cctgttgcca atcctaatac ttttgcacct
    14281 ttgttcatac cttctttgat gatttcaaat gctttttttc cgccttcttc agcgtttttt
    14341 acacgaatga tttccatagt gattgctcct ttatattttg gtatagccca attatagcat
    14401 tctttttttc tttttacaat aattagtata gaccaaattg catttttttc attttcttga
    14461 caataaatag gaatccgtgt taggtttcat aagatggttc aatcaaacaa tccatgttgc
    14521 atgaaaacca agcaagtgat tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa
    14581 aggagttatt atgagtatac tgacattaaa caacattacc cagcaatttg gcgacaaaat
    14641 cctctatgaa ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc tgattggaca
    14701 aaacggagcc ggtaaaagta cattgattaa aatcatcact ggtgagatct taccagatga
    14761 cggacagatc cattggcaaa aaaatattca caaaggttac ctcgatcaat atgtggaagt
    14821 cgatgaaaca ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa
    14881 ggaagaaaaa attgcttttc tttaccaaac atatagcgag accttcgatg attccttgct
    14941 ggaaaaagct gggaaactcc aaacggaatt agaccaagga gtgttttatc aaatcgacac
    15001 cttagttgct gagatgagca gcgggttggg gatcgatgtc ttaggtttag attcactgtt
    15061 gaaaaatcta agtggtggac aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa
    15121 cccagatgtt ctgatcttag atgaaccaac caatcactta gatgatcagc atatccaatg
    15181 gctgacgcag tttcttcagg attttgacgg tgcgtatatc gtgatttccc atgaccaaga
    15241 atttcttgat cagatcacca cccatatcgc agacatcgag tttggaaaga ttactaagta
    15301 tactggccat ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg
    15361 ccaatatcat gctcaacaaa aacaaatcga aaagaccgaa gcttatattc gaaaatacaa
    15421 agccggttcc cgatcaacga tggcaaaaag ccgacaaaaa caattagata aaatcgaacg
    15481 gctgacgcct ccagcttcag caagtaaagc tgtctttgat tttccattct cgcctattgt
    15541 cacgacatta gcagtggaaa caactgattt agtcatcgga tatgatcgac cgctacttgc
    15601 acccatcaat ttgaccatcc gctttggtga aaatattgcc attcgaggct tcaacgggat
    15661 tggaaaatct actttattaa aaacactgat cggtgagatt gaaaaaataa gtggcgattt
    15721 ccacttccca gataacacaa aaatcaatta cttttcacaa gatctgtact gggaaaatcc
    15781 attagaaaca ccactacaat atttaagcag tcaatttcca aaagcgacta tcaaagaatt
    15841 acgtcgccaa ctagctaaag cagggttagt taatcaatta gcaagtgaac ctctcactac
    15901 attaagtggt ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga accaaggtaa
    15961 tttactgatt ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca
    16021 agacgggata cagaaatttc caggaacagt aatcatcgtc tctcatgaac aagaatttta
    16081 tcaagatctt gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg attgaatagt
    16141 tgaaaaaaag gagctgtgac aaaagttttg tcacaactcc ttcttctgaa taaatggtgg
    16201 ccaattagct gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttta
    16261 tttggctatt cttattactt gatgcagaac agcttttcac aacctctttt cgtctatttt
    16321 ttattttcga gatttctctt tcctttaaaa acaactgttt gaaagaagaa cgcacatcct
    16381 tgaaaaacaa gcccgctaac cgttacacct aaccagccat actgctgcca cattgtcgtc
    16441 cctagcaatg aaccagccgc acctccaata aagtacataa acataaatac agtattattt
    16501 cggttgcttg ctgcttcacc tagcccttgt actcgtgttt gattggcgac ttgaccaaat
    16561 tgattcccga cgtctaataa aataatagct aaaagcaaaa tggccaaatg gcttccgcca
    16621 accaataaag caataaagct tattgtttgc ataacaagtc ccatatacac gatttttctt
    16681 tcagaataag aatctgccag tcgtcccacg ataggtgcag caactgctcc agacaaacca
    16741 aaaatcgcta atatccctgc ttcgaatgtt ccccaattgt aggcgggact gctgatataa
    16801 aaaatcaacg tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc
    16861 gcttctctta atagacgttg agaagcaact aaatgcggca aacttttaag tgaatcaagg
    16921 tatgaaacag ctgtactact ttgctggaca gaacgcggca ttttccagta aagcagaagt
    16981 gtaagaattc cgactgcaca taaagcaaat aaatagatca tccgccaaga aaaaagactg
    17041 gcaacaatac cagataccgt ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat
    17101 gtacccatga ccttccctct tgcttttggt cccgctagta ccgcaccgta aggaatgatg
    17161 atttgcggca cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac
    17221 gaaggcgcaa aaaaagcggc caacagagag acggcagata acgctgccat tcgaatgatc
    17281 aattttggac gatccaccac gtcccctaaa ggaactaaaa acagtaaccc taacgcatag
    17341 cctaattggg tcaacatcgt caatatcccg actgcacttg tgctaacaga taaactagct
    17401 gctacttttg ttccaatcgg ctggatgtaa tacatattgg caacaacaac gccacaagtg
    17461 attgataata gtaaaataag cgaagttgtt aaaggttttt tttcattact cttcattgat
    17521 ttcccccatt tcctatctta gtttacaact tatggaaaag attggaaata gaaaaataca
    17581 actattcgcc acacttatta tttgtgcttt tgttttcctt gttttttctt gcgatcttta
    17641 taaatgaaga caactaaccc tactagcaca gtaacacttg caaccaacgt gactgcagtt
    17701 ttgttatatc cgtgaaacag agcttctcca ccacacgcat acaacacaga cgaagggatg
    17761 actccaatca cgatcgacaa tatgatttgc tgtagaggca atttcatcgc atctgcagca
    17821 aaattgacca tagaagatgg aataaccggt atcatatagc ctaacattac acctattttt
    17881 ggatgtttca tttgtcggat tgcttgcacc caatgattcg tttcggtttt cttatctaaa
    17941 aattttaagc gatgcataag gaaaattgca gtgatgttac caagagcatt acccaaaatg
    18001 ttgatgatcg cacctgtcag tggtccaaag ctcaaaccag caatgactcc aactactgaa
    18061 gtaggaatac ctggaacagc acacatcacc gctgtcaata cgatcaagag tatcgatgca
    18121 aaagtcccat gcgaacgaat atgtgcgagt agctctgcct tactcgctct cggatttaaa
    18181 aataattgga tgtctgtctg atattctaaa tacaaccgat agcccactac cagtatcaga
    18241 atgattccca acgagatcaa tatcaatcgg attattttct ttttcatatt tcctcctgaa
    18301 aaaaatctat attcattaaa taatagttta acataaaaac aaaaaagcac cgaaatccaa
    18361 cataggatct cagtgctttt aaatagtaaa aattatttaa cagttacaga tgcgccaact
    18421 tcttcaagtt tagctttcaa ttcttcagct tcttctttag atacgccttc tttaactggt
    18481 gcaggagcgc catcaactac agctttagct tctttcaagc ctaaaccagt tgcttcacga
    18541 actgctttga taactttaac tttttggtct ccagctgcag ttaattctac agtgaattct
    18601 gtttgttctt cagcagcagc agcgccacca gcagcagcta cagctacagg agcagcagca
    18661 gatacgccaa attcttcttc aatagcttta actaggtcgt taagttctag gatagttgct
    18721 tcttttaact cagcaacgat gttttcaatg ttcaatgcca ttataatttc ctccgtttaa
    18781 ttgttctttt ttatttaatg ctttatgcgg caattaagct gcatctccat cttctttgtc
    18841 tgccactgct ttgacagcgt aagccacgtt gcggactggc gcttgtagta cagatagcaa
    18901 catagataga agtccttcgc gatttggcaa ttttgccaat gctgtgattt cttccaatga
    18961 agagacttta ccttcgatga taccgccttt gatttctaat gcttttgctt ctttagcaaa
    19021 ttcgtccatg attttcgctg gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg
    19081 tccagtgaat acttcgtcca tgccttcaag gccagctttt ttagctgcac gtgaaagaat
    19141 agaatttttg ataactttca tttctacgtt agcatcacga agttgtttac gtaaacgtgt
    19201 tacttcatca actgttaatc cgcggtagtc aacgactact actgatgcag ctgctgtaaa
    19261 tttttcgctt acttcatcaa cgattgctgc tttttttgcg ataattgctt cactcatttt
    19321 tgagtttcac ctcctgaatt ttgatggaag agttttttgc actgaaaagg caagtcactt
    19381 ttatagttac tttgctcaac cagtattatt catcacctat tcacttcgtt catagtgatt
    19441 ctactcgctt tgctcgaaag gaaactcctt tcgctttgct caaccagtat tgttcatcat
    19501 ctcttcactt cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag
    19561 ggactattga atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta
    19621 ctgtcttcgg tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag
    19681 tcaagaatta tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt
    19741 ggtcaacatg gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac
    19801 ctttagctgc tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa
    19861 ctaatttttc atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt
    19921 ccacacggta agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg
    19981 taactgtacc agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac
    20041 gacctacagt agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt
    20101 tttggatttt ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt
    20161 cttttgcttt gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg
    20221 gtaatacaac agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt
    20281 atgcaacttc aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg
    20341 cttcggcaac aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt
    20401 tgctcttttt agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac
    20461 gtgttctata tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat
    20521 tattctacag tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca
    20581 acgttagctg cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta
    20641 gaaactttcg caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct
    20701 tttttcaata gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg
    20761 tatacagaaa taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat
    20821 tcttttgtga atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga
    20881 gctggagttg ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc
    20941 acgagacata cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc
    21001 acgttttatc tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta
    21061 cagtatagca aatttatttg ttttttcaag atttttttct gctattttta cattttgctt
    21121 gaataaacgc taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta
    21181 tgttacactt gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag
    21241 acccttgtac aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt
    21301 atagaagtgt ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg
    21361 ccggagctgc tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg
    21421 tagatattta tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg
    21481 cattagttgg cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga
    21541 aattggcata tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac
    21601 atccaaactc tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca
    21661 tttcgatcgg cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa
    21721 gcatgggtac tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag
    21781 tgactagtat actttctgag accaatacga atatcggcac tatgacagtg acacgggaat
    21841 caaaaggtga aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg
    21901 taaaaagact caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta
    21961 aaaaaatgtt tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag
    22021 cagaattgat gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg
    22081 agatcatgga aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg
    22141 ttacctccgt cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg
    22201 aggcagactt tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta
    22261 atgaagtcaa tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg
    22321 ttgttcctgg tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat
    22381 tggaattctt atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca
    22441 gtggtgcaga agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag
    22501 ctttggtttg tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat
    22561 tgaaaaatat gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg
    22621 tcaaacgtaa tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg
    22681 gtatcgaaag tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc
    22741 aaatgccgtc gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta
    22801 aacgacttgc ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta
    22861 ttaagagatt agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc
    22921 gttttttatt aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca
    22981 agttgcatcg tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct
    23041 gccccaccta gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca
    23101 cttgccagag ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt
    23161 acacgtttgt tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca
    23221 tctagttgac gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg
    23281 attactgcat cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct
    23341 ttgattgttt tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg
    23401 attccttttc cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc
    23461 attggtaatg gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt
    23521 gaatctgccg ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac
    23581 tcattgaatc tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct
    23641 gcatcggctg cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca
    23701 gcagcagcta ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc
    23761 gctgatggcg cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa
    23821 tcggctgagt cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc
    23881 gtattttctt tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct
    23941 gcttccatgc gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga
    24001 tgttcttcat tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca
    24061 tataaatcat actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg
    24121 ctattctttc caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat
    24181 actatgagag gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt
    24241 caacaacgca tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt
    24301 ttcatctagt tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat
    24361 ttgctggtaa tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt
    24421 cagagactgc gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt
    24481 ctgaacttgt ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac
    24541 tcctcccatg gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata
    24601 caaaagacaa gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa
    24661 caatacttgg accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg
    24721 tccatctgtt cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg
    24781 ccgcaacatc cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg
    24841 aatagtggtt ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc
    24901 agtagtattt atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa
    24961 agctgctgca gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct
    25021 ttccatatag aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc
    25081 tttattaatc gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa
    25141 acatatcgat gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt
    25201 ctaatccagc aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat
    25261 cattttgtcg caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta
    25321 atggtgctgg tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt
    25381 tgcggacaac ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga
    25441 atgttttatg aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag
    25501 gaacaacgac acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat
    25561 ttgcttttac tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa
    25621 aagattccat tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa
    25681 gtttttcttt cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa
    25741 atccagccaa ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata
    25801 atagaagttt cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt
    25861 acttgtttga tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta
    25921 ctgtttattt cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga
    25981 tttccaaacg ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac
    26041 aaacggaaca agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga
    26101 atcagactta tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa
    26161 actatctatt gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag
    26221 acaattggag gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca
    26281 accgttacag agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac
    26341 cgttgtgtgc cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg
    26401 catatctatc gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg
    26461 aaaaacgcta caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc
    26521 aaagagcttc cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca
    26581 catagtacgt tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta
    26641 ctgacaattg cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca
    26701 aaacacagca gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga
    26761 cgtacattga tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac
    26821 cacttagaga aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca
    26881 taatcaaaaa gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt
    26941 agacacgctt ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag
    27001 ccatgacgat aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac
    27061 ctgttggaac taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca
    27121 atactaaacg catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag
    27181 aaagcaatgt ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat
    27241 tatagacgat gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat
    27301 gattcaaact ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa
    27361 tagttaacgc tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt
    27421 gtctgctttt cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga
    27481 tggttgatga accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt
    27541 tcaatccgtc ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa
    27601 aaatcgtggg atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag
    27661 acgaagtagg atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt
    27721 gggaatttga gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg
    27781 acatgccgaa ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt
    27841 ttttgtgttt ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca
    27901 agaattaatt ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga
    27961 aaaagcttgc taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt
    28021 gaatagcagg aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc
    28081 agcggaaatg gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt
    28141 gactatacac ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg
    28201 gcaaaagaaa cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc
    28261 cttttgatca tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag
    28321 atggaagtag aattcccagt tgcttatatt ccagcaggtt ctggagatga ttttgctcga
    28381 ggggcaggcc ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca
    28441 ccagaaaaag tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct
    28501 gttaataatt tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc
    28561 acaaaaaagc gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc
    28621 cgtgctttat tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta
    28681 agtttttcaa acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc
    28741 gcgattgttc caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga
    28801 atcaatttct ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa
    28861 tcgaaatatt tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa
    28921 catggtcaag aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca
    28981 actaaacagt tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac
    29041 cgtttattcg gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc
    29101 agttaattct ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat
    29161 tggatcattg aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat
    29221 tctgcaaatg tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac
    29281 caatatgttg gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt
    29341 acaaacaccc caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg
    29401 gtttctgagt tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa
    29461 aacaagaaag tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact
    29521 aacagaacca ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta
    29581 aaggttactt tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg
    29641 atctctttgc gattaaatgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac
    29701 aacccataag acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta
    29761 tatcccgcac ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata
    29821 gctgcatcga ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc
    29881 acttgctgtg taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc
    29941 ggcatctttc ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt
    30001 acacgattaa aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg
    30061 actgtttcct tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca
    30121 ctaatcaagg cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca
    30181 aaaaagaatg tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct
    30241 ttaagcaatg tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc
    30301 atttcctaac gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact
    30361 agccaaatca gtgtagaaat aaagtttagg cggattttat aatgaactta gccatttaaa
    30421 ataaaaaaaa cgccacgtga attccatgtt atattgaagt taccacaact ctcaatagac
    30481 aaggatgaat tcacatgacg caaacccatg ataacacagt ttcacgcaag ggaaaacatc
    30541 tttcctactc agaacgttgc caaatcgcta tcctgaaaaa ggaaggctat tccaaccgtc
    30601 aggttgccaa agtgctggga cgtgttccgc aaaccataaa caatgagatt gatcgcggaa
    30661 ccatcaccca gttaaaacgc caaacgcaaa atggaaaagt gtatgacacc tactattctg
    30721 tttatgacgc tgatgcagga caggccttct atgaaaagca acgactgaac tgtggcagac
    30781 gccccaaatg ggcgaacaca aatgccttca ttgattgggc agatgacaaa atgttaaatg
    30841 aaaaatggtc gccggacgtc gtggtcggat ttgccagaaa acacgagctg ttcgattgtg
    30901 ccatcattcc tggcacaacc gctctttatc aatggattga ccgcggaatc atgcgaacaa
    30961 agaacatgga cttgcttgaa aaactgtccc gtaaaatcaa agcggtccgc ccaggacacc
    31021 gtcccaacaa gcgtgttctc gggacgtcaa ttgaagaacg ccctaaagaa gttgaaacac
    31081 gggaaacgtt cggtcattgg gaaatcgaca cggtagtggg aaacaagacc aagtcggatg
    31141 ccgtgctgct gacgctggct gaacggcaga cacgctttga agtcatccta aagttgaacg
    31201 ggaaggacac cgaatcggtg gaccaagcgg ttcaacagct aagagaacgg gctggcgcta
    31261 acttcgatca attatttaag accgttacat ccgacaatgg ctcggagttt tctgggctac
    31321 acgatgctct gcaagatgta gctgacgtct atttcagcca cccctatgct tcttgggagc
    31381 gtgggacgag cgaaaaccag cacaaattaa ttcgccgttt cgtccccaag ggaagcccga
    31441 ttagtgaggt gagcgagaac cagattttgc ggatccagcg ttggatgaac gattacccgc
    31501 ggaaaatttt gggttatgcg acaccacatg atacattcgt ccaggcattc aagcaagaac
    31561 ggttggtggc ttgacccatt attccttgat aacgatcctc actcgggaca tggcttcatg
    31621 tctggaaagc tggcctgggg ccagaatcgt ctgaagacta tcatacctca gttcattatc
    31681 aaggcctcac tgatttagaa taacgaggca gttacggtat caaacaaatg agttacctgt
//
LOCUS       DASJOX010000020        31088 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJOX010000020 DASJOX010000000
VERSION     DASJOX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.020"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..345)
                     /locus_tag="U4A12_000885"
     CDS             complement(<1..345)
                     /locus_tag="U4A12_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773292.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSP"
     gene            612..2312
                     /locus_tag="U4A12_000886"
     CDS             612..2312
                     /locus_tag="U4A12_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN1773293.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2282..2500
                     /locus_tag="U4A12_000887"
     CDS             2282..2500
                     /locus_tag="U4A12_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN1773294.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2520..3410
                     /locus_tag="U4A12_000888"
     CDS             2520..3410
                     /locus_tag="U4A12_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN1773295.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3428..3712
                     /locus_tag="U4A12_000889"
     CDS             3428..3712
                     /locus_tag="U4A12_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773296.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4199..4813
                     /gene="gmk"
                     /locus_tag="U4A12_000890"
     CDS             4199..4813
                     /gene="gmk"
                     /locus_tag="U4A12_000890"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN1773297.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4818..5123
                     /locus_tag="U4A12_000891"
     CDS             4818..5123
                     /locus_tag="U4A12_000891"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1773298.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5376..7784
                     /gene="priA"
                     /locus_tag="U4A12_000892"
     CDS             5376..7784
                     /gene="priA"
                     /locus_tag="U4A12_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN1773299.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7799..8290
                     /gene="def"
                     /locus_tag="U4A12_000893"
     CDS             7799..8290
                     /gene="def"
                     /locus_tag="U4A12_000893"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1773300.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8283..9221
                     /locus_tag="U4A12_000894"
     CDS             8283..9221
                     /locus_tag="U4A12_000894"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN1773301.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9221..10579
                     /gene="rsmB"
                     /locus_tag="U4A12_000895"
     CDS             9221..10579
                     /gene="rsmB"
                     /locus_tag="U4A12_000895"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN1773302.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10592..11332
                     /locus_tag="U4A12_000896"
     CDS             10592..11332
                     /locus_tag="U4A12_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN1773303.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11329..13398
                     /gene="pknB"
                     /locus_tag="U4A12_000897"
     CDS             11329..13398
                     /gene="pknB"
                     /locus_tag="U4A12_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN1773304.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13563..14462
                     /gene="rsgA"
                     /locus_tag="U4A12_000898"
     CDS             13563..14462
                     /gene="rsgA"
                     /locus_tag="U4A12_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1773305.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14475..15125
                     /locus_tag="U4A12_000899"
     CDS             14475..15125
                     /locus_tag="U4A12_000899"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1773306.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15126..15764
                     /locus_tag="U4A12_000900"
     CDS             15126..15764
                     /locus_tag="U4A12_000900"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN1773307.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15919..16773
                     /gene="mreC"
                     /locus_tag="U4A12_000901"
     CDS             15919..16773
                     /gene="mreC"
                     /locus_tag="U4A12_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN1773308.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16777..17286
                     /gene="mreD"
                     /locus_tag="U4A12_000902"
     CDS             16777..17286
                     /gene="mreD"
                     /locus_tag="U4A12_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN1773309.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17541..19115
                     /locus_tag="U4A12_000903"
     CDS             17541..19115
                     /locus_tag="U4A12_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1773310.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(19183..20982)
                     /locus_tag="U4A12_000904"
     CDS             complement(19183..20982)
                     /locus_tag="U4A12_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN1773311.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21105..21611
                     /locus_tag="U4A12_000905"
     CDS             21105..21611
                     /locus_tag="U4A12_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1773312.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21690..22412
                     /locus_tag="U4A12_000906"
     CDS             21690..22412
                     /locus_tag="U4A12_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773313.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(22490..23890)
                     /locus_tag="U4A12_000907"
     CDS             complement(22490..23890)
                     /locus_tag="U4A12_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN1773314.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(24060..25034)
                     /locus_tag="U4A12_000908"
     CDS             complement(24060..25034)
                     /locus_tag="U4A12_000908"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1773315.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            25387..25947
                     /locus_tag="U4A12_000909"
     CDS             25387..25947
                     /locus_tag="U4A12_000909"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN1773316.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            25964..29485
                     /gene="mfd"
                     /locus_tag="U4A12_000910"
     CDS             25964..29485
                     /gene="mfd"
                     /locus_tag="U4A12_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN1773317.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            29500..>31088
                     /locus_tag="U4A12_000911"
     CDS             29500..>31088
                     /locus_tag="U4A12_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1773318.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT        10058 a         5724 c         6687 g         8619 t
ORIGIN      
        1 cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg
       61 tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc
      121 acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt
      181 cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt
      241 ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac
      301 agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt
      361 atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat
      421 tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt
      481 ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc
      541 tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa
      601 aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc
      661 ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg
      721 ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg
      781 tctattttac tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag
      841 gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta
      901 ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt
      961 ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag
     1021 cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc
     1081 tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg
     1141 gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga
     1201 caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct
     1261 atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc
     1321 cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa
     1381 tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc
     1441 aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg
     1501 ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa
     1561 tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag
     1621 ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa
     1681 cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac
     1741 aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat
     1801 ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag
     1861 aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt
     1921 tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa
     1981 aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct
     2041 gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt caattaagta
     2101 ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa
     2161 ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc
     2221 ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac
     2281 catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa
     2341 caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga
     2401 tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga
     2461 agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa
     2521 tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata
     2581 ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc
     2641 tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa
     2701 tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact
     2761 gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta
     2821 gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgtgctggac gaaaacttcg
     2881 taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata
     2941 cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag
     3001 ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt
     3061 ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc
     3121 ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg
     3181 gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta
     3241 tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg
     3301 aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt
     3361 ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt
     3421 agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa
     3481 acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta
     3541 taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa
     3601 atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg
     3661 aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg
     3721 ctgaacaaag aaatacaata aataaacaga attggatttc ttcgcttagc ttctttgctc
     3781 taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata
     3841 agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa
     3901 ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat
     3961 acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga
     4021 agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt
     4081 ggtacagggt agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc
     4141 agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat
     4201 gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt
     4261 acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac
     4321 ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga
     4381 gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta
     4441 ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt
     4501 ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat
     4561 ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga
     4621 tgcagatgaa gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc
     4681 tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa
     4741 cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt
     4801 aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa
     4861 attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg
     4921 cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa
     4981 tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga
     5041 tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta
     5101 tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca
     5161 aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc
     5221 tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg
     5281 gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct
     5341 tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg
     5401 tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg
     5461 aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg
     5521 tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg
     5581 cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg
     5641 attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga
     5701 gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc
     5761 aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc
     5821 tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata
     5881 cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg
     5941 aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt
     6001 tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg
     6061 ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc
     6121 gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac
     6181 aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt
     6241 tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg
     6301 ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa
     6361 tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt
     6421 cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag
     6481 taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg
     6541 aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag
     6601 caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt
     6661 tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag
     6721 ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga
     6781 atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca
     6841 aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta
     6901 gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg
     6961 atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc
     7021 ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg
     7081 aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa
     7141 gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat
     7201 tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt
     7261 tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat
     7321 tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc
     7381 aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt
     7441 tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg
     7501 tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg
     7561 ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca
     7621 tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac
     7681 cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag
     7741 gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat
     7801 gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga
     7861 tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa
     7921 tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga
     7981 agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga
     8041 aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc
     8101 agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt
     8161 tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat
     8221 tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct
     8281 tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc
     8341 tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc
     8401 ggaaacgagt aattacgccg acaccggtaa aaaaagcggc tctgaagcac ggtatccgtg
     8461 tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc
     8521 ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac
     8581 caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc
     8641 ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga
     8701 tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag
     8761 atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa
     8821 cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt
     8881 tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa
     8941 ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca
     9001 tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta
     9061 cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag
     9121 ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg
     9181 gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa
     9241 aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg
     9301 cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga
     9361 tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt
     9421 ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agatgcttct
     9481 ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa
     9541 cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg
     9601 tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat
     9661 cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac tggtggatca
     9721 aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag
     9781 tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga
     9841 agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca
     9901 tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat
     9961 gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc
    10021 acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt
    10081 ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct
    10141 aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt
    10201 cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct
    10261 tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa
    10321 aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac
    10381 ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga
    10441 aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat
    10501 cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg
    10561 ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg
    10621 aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa
    10681 gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat
    10741 ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat
    10801 cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca
    10861 agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga
    10921 ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat
    10981 catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag
    11041 cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc
    11101 tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt
    11161 atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca
    11221 agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg
    11281 agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg
    11341 ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca
    11401 aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga
    11461 tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca
    11521 acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg
    11581 cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat
    11641 tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct
    11701 ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat
    11761 aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg
    11821 atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga
    11881 ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg
    11941 ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt
    12001 caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac
    12061 gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg
    12121 tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct
    12181 catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct
    12241 gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag
    12301 gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta
    12361 ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga
    12421 ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc
    12481 gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa
    12541 gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa
    12601 gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa
    12661 agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa
    12721 aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca
    12781 gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca
    12841 gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt
    12901 gttacacaag gattcaatat caataatatt agtgctactt atgactatag cgacactgta
    12961 ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact
    13021 tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat
    13081 acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag
    13141 acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca
    13201 ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca
    13261 gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt
    13321 agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc
    13381 tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt
    13441 gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg
    13501 agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg
    13561 aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg
    13621 caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc
    13681 cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg
    13741 aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa
    13801 tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag
    13861 ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag
    13921 acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga
    13981 ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat
    14041 cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg
    14101 aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac
    14161 gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca ggatttagct
    14221 cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag
    14281 aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag
    14341 taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat
    14401 tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat
    14461 aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac
    14521 aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg
    14581 atggccgatt tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg
    14641 ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag
    14701 catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc
    14761 atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag
    14821 gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga
    14881 ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg
    14941 aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa
    15001 tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcaggat
    15061 catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag
    15121 actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc
    15181 tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag
    15241 gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg
    15301 aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata
    15361 cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg
    15421 gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc
    15481 gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt
    15541 atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta gcttattgct
    15601 gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag
    15661 aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt
    15721 ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa
    15781 aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag
    15841 aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa
    15901 ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg
    15961 gttattgtta tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa
    16021 ggaaagccca atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca
    16081 atctctgccc cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca
    16141 acttatgccg aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag
    16201 caaaacaaga attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag
    16261 acgctgacta gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg
    16321 caggacatgc tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc
    16381 atgtcgcaaa aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc
    16441 gaattactgt catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct
    16501 aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg
    16561 acccaattaa ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt
    16621 ggagggaatt ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt
    16681 tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt
    16741 gtgacgattg tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag
    16801 tactatctgc caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg
    16861 ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta
    16921 ttatgttgca gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg
    16981 gctatctatg acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta
    17041 attgtatggt tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt
    17101 ttcagtatga tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt
    17161 tttaaattag cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacactg
    17221 ctgttgaaca tgattatatt tgtattattc atttttccct ttaagaaatt attcagtgat
    17281 gaataatttc tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga
    17341 aatgctagac ttaacgcggc tttttcatga tcattttctg aaacgaacag ttttgtcatt
    17401 tttaaaagtt gttttgaaac atttttgtaa cataactatt atatcgttga cacactgcta
    17461 tggtacaatt tttatatcgt taagaaagac ttaaatatat ttagatattt attaaattaa
    17521 gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa
    17581 tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt gattctcaga
    17641 tacaacagca agatcaaaag attgcagact tgaaaaatca acaagcagat gcacaatcac
    17701 aaatcgatgc cttagaaagc caagtttctg aaatcaacac acaagcacaa gacctattag
    17761 caaaacaaga tacattgcgt caagaatctg cacaattagt aaaagacatt gcagatttac
    17821 aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa
    17881 gcaacacaag ctcaaactac atcgatgctg tattgaatgc tgattcatta gcagatgcta
    17941 ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa caacgacttg atggaacaac
    18001 aaaaacaaga taaaaaagct gtagaagaca aaaaagctga aaacgatgct aaactaaaag
    18061 aattagctga aaaccaagct gctttggaat ctcaaaaagg cgatctttta tctaaacaag
    18121 ctgatttgaa cgtactgaaa acttcattag ctgctgaaca agcaactgct gaagacaaga
    18181 aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac
    18241 aacaacgtct agcagaacaa gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag
    18301 cacgcgaaca agcagaggct gaagcacaag ctactcaagc ttcatctact gcacaaagct
    18361 cagcaacaga agaatcatca gctacacaat cttcaatgac tgaagaatca tcatcagcta
    18421 cacaatcttc agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag
    18481 cgcctgaatc ttcagcaaca gaagaatcaa caacagcgcc tgaatcttca gcaacagaag
    18541 aatcaacaac agtgcctgaa tcttcagcaa cagaagaatc aacaacagtg cctgaatctt
    18601 caacaacaga agaatcaaca acacctgcgc ctacaacacc atcaacagat caaagtgttg
    18661 atacaggaaa cggcacagga agtagtactc cggctccaac gccaacacca acacctgaac
    18721 aaccaaaacc tgtaacacct gctccagctc cttctggaag tgtgaatggt gcagcaatcg
    18781 tagcagaagc ttacaaatat attggtactc catatgtttg gggcggtaaa gatccaagtg
    18841 gctttgactg ctcagggttc acacgctatg tttacatgca agtaactggt cgtgacatcg
    18901 gtggttggac agttcctcaa gaatcagctg gaacaaaaat ttcagtttct caagcaaaag
    18961 ctggagactt gttattctgg gggtcacaag gcggaactta ccacgtagcg attgctttag
    19021 gcggaggaca atatatccat gctcctcaac caggcgaaag cgtaaaagtt ggttcagttc
    19081 aatggtttgc ccctgacttt gctgtcagca tgtaagaatg attaatcaaa aagagttagc
    19141 ccattctgga ctaactcttt ttttattgtg ataaaataga aatcagataa attcttctgt
    19201 caattccatg aacgtttgga tatcttcagt caccatgtcg atacctgctt gccaaaaatc
    19261 tggttgggta agatcgacat tcaaatgttt cttcgcaaga tcttccgtcg tcattgaggc
    19321 agtatcgcgt agtaaatgga tatattcttc ttcaaaattt gctccctttt ggttggcgta
    19381 agcatagatg cccaaactaa agaggtagcc aaatgtgtat gggaagttat aaaacggtac
    19441 gtcatcaata aagaaatgca attttgcagc ccagaaatat ggatggtaag tagctaaagc
    19501 tccttgatag ccttcttttt gcgcctcaac catcatctct gtgatcttat cttctgagac
    19561 taatccttct tgccgtgctt catagaatcg attttcaaaa atgaatcggg aatggatatt
    19621 catgaacata gctaaagcat tttgcagttt tgtatctagc aaattgattt tttcttcttt
    19681 tgtttttgcg acttttaatg tagcatcggc tacgatcagt tctgcgaatg tacttgctgt
    19741 ttctgcgaca ttcatggcat attcgcgatt taaagaaggt aaatcccaca tcgtgctgct
    19801 atggaaagca tgtcctagtt catgagcaag tgtcgcaacc tcattaacag aattactgta
    19861 ggtcataaag atccttgatt cttttgtttc tggtaactca gtacaataac caccaggacg
    19921 tttcccagga cgatcttctg cttcgatcca gctcttttca aaagcagatt gggcaaattt
    19981 agccattttt ggactgaatt tgttaaagtt ttcgataata aatgctgctg cttcgtcaaa
    20041 actaaaggtt ttttctttca aatcgcctaa aataataggt gcatcttgat cttgccattc
    20101 cattttttct ttaccgaaaa gattagcttt tctagtcaag taattaacaa ggggttgttt
    20161 gtttttctgg attgtatccc acatgacaga caatgtttct ttcttcaacc gattgtactc
    20221 taatggtttt tgtagaaaat ctgtgacacc atgaagttca tagtcagaaa gtctaaaacc
    20281 atctaaatga ttcaaagtat ccgtgaagag gggtgccttt tctttccagg cttcttccca
    20341 tgctgcgaaa agtgtttctc tgacttttgg atctggatcg cccatcatct tattgaaggc
    20401 ttgtccagca gataattcga ctacttgtcc atcttgttcg aagggaatcg aaatgctggc
    20461 tacaatcgta tcataatgac tgctccaagc atttagacca tctaaagaaa gcgtatttat
    20521 aatgttttct tcagcttctg ataataattg tgagccatca cgacgaatct cgtttaaacg
    20581 aaaagcaatt gtttcaaacg aagattgaga aagcatctga ttccaatcat tgtcagatat
    20641 ttctgcaaat ttctttgaca atacagtttc ggctgattgc agacgaggca gcattgcgta
    20701 aagcttgcct gataagacct ttgcatctga atcattgaca ttggcagaaa gcaatgcagt
    20761 aatatagctg ctgcattggg taaaaccatt cgtaatcgtt tcttgcaatt gaagaatagc
    20821 gtttaactgc tctgcattgt cagaactagg tgaccatttt gttacgcggt gataatactc
    20881 gttcatttgg tcttccaatt gcttcatccg ttggttcaaa gcatcagaat gactgcctcc
    20941 ggaaaaaata gaatctagat tccaatttaa tgaataactc atgaaaaaac tcccttcata
    21001 ttgtaataca aaattaagta tagcactttt ttatggaaga agtctctatg aaaatgattg
    21061 ttgtttcttc aaaaaaagtc taagataaaa tacagaggtg acttatgaat tcttcaaaaa
    21121 aacaaatgaa aaacggaaat atttatttag taatcgcact gatagtcgtt ataatcttat
    21181 tttacagttc ttctcaaact tacggacagc aatcacaagt ttcgcgatta gaaagctggc
    21241 tgcctaatca gccatttaaa gacacattag cgggtatacg ttttaattat ggtggaagta
    21301 tggtgagtat caaccatctg ggctacgcca aatttttaga gttcttttta aggaagggcg
    21361 cacatttcgg tacctatttt attttgggag gcagcttgtt tttaggtgtt ttccccaagc
    21421 taaaaatttg gtggctgaca gcgatccttg cttggctttc agcaacaggg tacgcaggtt
    21481 tggacgaatt ccatcagatg atgaccggtg gacgaacacc tatgttccaa gatgtcatgt
    21541 tggattcgat cggtgctctg actgctgtga tattatgtgt gctttttgcg gcattcaaga
    21601 agcgtttata gattggctgt ggctttgttt ttttgtgaaa tagtactaga atagaagtgt
    21661 attctcaaaa gaacaaggga ggaaaaatca tgtcaggaca tagtaaatgg cataatatcc
    21721 aaggaagaaa aaatgcccaa gatgcaaaac gagggaaagt ctttcagaaa ctttctcgtg
    21781 aaatttatat ggcagctaaa gcaggcggtc ctgatccatc tatgaatcca gctttgcggc
    21841 ttgttgtgga caaagcaaaa gctgcaaata tgcctaacga caatgtggaa cgggcgatta
    21901 aaaaggctac gagtgccgta gatgaaacga attacgatga aatcacgtat gaaggatatg
    21961 gtccgggtgg agtaggaatc ttagtccatg cattgacaga taaccgtaat cggacagcga
    22021 caaatgtccg agtagctttt actcgaaacg gtggatcact gggagaaacg ggttcagtca
    22081 gttacttgtt tgaccgcaaa ggatatatcg ctattgaacg cgatgggttg actgttgatg
    22141 aagagacgat gtttgaggat gtattagagg cgggggcaga agatctgcaa acttctcctg
    22201 aagtattcga gatttatact gcaccagaag attttgctgc tgtacgagat gagttggaaa
    22261 aagagttcac tttggcgcaa gcagaattga cgatgattcc acaaacgacg attgatttaa
    22321 atgaggaaca aaaagagcaa ttggaacgtc tagtagataa gctagaagac gatgatgatg
    22381 tttcagaagt ctttactgct gctgaaatgt aagtagcaca ttgttagata ataaaaagaa
    22441 agcacctgta ccaatcgtac gggtgctttc tttttatata ttatttttgt tagaagacca
    22501 atacaggcgt tcctttttcg accatcccga ataattcctt cattacactt ggtggggtgt
    22561 tgatgcagcc gtgtgaaccg cgggttttcc ataagtctcc accgtattca ggttgccagt
    22621 ctgaatcgtg gataccaaca cctgtccagt caatcggcat ccagtagttt accggactct
    22681 cgtaaggtgt tccatcgtca tttgttcctt ttaaagttgc atcttcttct ttattccaaa
    22741 catagaagac tccagcaggt gtaggtgtag ttggctttcc ggaaacgata tctgtttcaa
    22801 gggccacttt tccgtctttg taataccaca tatgctggtt ctccagatca acttcgatat
    22861 acgtatcttc gatcaatgga tgatcagcgg ttgtgccacc ttgcacaatg ggtgaacgag
    22921 taaaatcttg gcctgccaga atcgcttttt tcaatgcttc tgtctcactg tctgtttgga
    22981 tcgtccaact gtaagtgcca actgggaccg taacttctcc tctttttgta cttttaaatt
    23041 ttgtgtcatt cgtacttgtg ttatactttg tgcctaaatc tgttacatat tgacgtactt
    23101 gttctgtatc taaatcgact tttccatcat tgtaggtcag ccaactcata atatcacttg
    23161 atgggatctg gaaagtctca ccgtttatac tgtaagtagc ttttacattt gcgatagcat
    23221 tcatggatgc gagttgttct ttcagtgagc tgtcttcaga tgttactttt ggtttttctt
    23281 tgaattcagt caattcaatt gtatctttac cagagtttac cgcacttttt aattgtttga
    23341 ccgcagcatc gacgtcgatc gtatttccat tgacttcagg cttgataacg aaagagtcac
    23401 ctgctttttc gatcgttgca tcagtagtag gtgtccggtc tttgttcaaa tcagtcagct
    23461 tagttgtcaa tgtctgaacg gtagtgtcta gtttttggtg gttttgactg acaggatcta
    23521 tttcttgttt ttctgccgct gagacataag tcattcccca agtccatccg ttttgttcat
    23581 ttaggatcgg agaaagctcc gaagtgaaat catctttgta gccgagctcg gattttggta
    23641 cttcctgcca gacttttcca tcttctttaa tagccagttt tttatcagaa taagcttctt
    23701 ttaatttttc atttgctttt tcgactgtta gattgctgac atttattcca tttatttctg
    23761 tattcggcaa aaaatgcttt gtgtagtatg tactgcgata ggtataaaag ctgatcagca
    23821 ctaagagaac accaagaact gaaagcaaag caacatgttt cgattttttt cgatgcgaag
    23881 atcttgtcat atcgacaact ccttaagtaa aagtttaggg tggaacggtc tcatgatagc
    23941 ataatttaga ggaaaagacg aaggttatct ctactttatg aaaaaattta gaaaccataa
    24001 gggtgataaa ataaaaaaac ggaaaacacc gaatagatgt tttccgtcat gttcaaagat
    24061 tattcagcgt ctagtttagc aaaagcttca tctagaattt cttttaattg cttagcagaa
    24121 gcagccatac ggtcttgctc gctgtcactt aaaggaattt cgattacttt ttggatacct
    24181 tgacggttga taacagcagg tgcaccaatg tagatgtcgt tcaatccata ttcgccattc
    24241 atgaatactg ataaaggaag aacagcattt tcatcatcta agatggcacg agtgatacgt
    24301 gctaatgcta ctgcgatacc atagaaagta gcgccttttt tctcgataat tgtgtaagca
    24361 gcgtcacgta cgccaaagaa taggttaacc atagcttctt catcgatttc tgggttgttt
    24421 tttacccatt catagatttg caagccggca acatttgcat gtgaccaaac tgggaattct
    24481 gaatctccgt gttcacctaa gatgtaagca tggacgttac gtgcatcaac gtcaactaat
    24541 tccgcaagtg cttgacggaa acgagctgag tcaagagagg tacctgaacc gattacgcgt
    24601 tctttaggga aacctgagaa tttccaagtt gagtaagtca aaatatctac tgggttagcg
    24661 gcaactaaaa agataccgtt aaatcctgat tcaacgattt gtgtgacaac atcgcggttg
    24721 atttttaggt ttttgtttac taagtctaaa cgagtttcac ctggtttttg tggtgcgcca
    24781 gctgtaataa caactaagtc agcatcatgt gcgtctgcgt aagtagcaga atagattttt
    24841 ttaggtgaag taaatgccaa agcgtgagac aagtcaattg cgtctccttc agttttagca
    24901 gtattgatat ccacaatacc tacttcttga gcgatatttt gagtaactag agcaaaggca
    24961 tagctagaac ctacggcgcc gtctccaaca agaataactt tttgatgatc ttttttttct
    25021 gcagctgcag tcatttagta tcaatccttc cattgatttg ttttttccca tgcttatgct
    25081 aacatttctc aagccccatg tcacgctatt tggctataaa atataacaga taacaaggtt
    25141 gtattatatg taaaagctta catgtgcgtt atttcactat ttttatatat caattatttc
    25201 attaaacttt gaagaaattt ttaatttctt tatcatttat taacgattct cttttagccg
    25261 aaacgccttg ttttgtgtta tactatgcat tgacaagtgt aaaaaagaaa aaagctcgat
    25321 ggactggacc gctaattagc agtccagcct tttgtgctgc tttttagaat taggtggaaa
    25381 acaaaaatga aaatgatcgt cgggttagga aacccaggta ccaaatacca atatacaaaa
    25441 cacaatatcg gctttatggt cgtggataaa attgcacgcg agcatcaagc tactttcaaa
    25501 aaaaatccgt tcgaagcaga agttgccgaa ttttttcata atggagaaaa gatcctttta
    25561 gtcaaaccac aaacattcat gaatgaatct ggtcgtgcag tcggcccact aatgacttat
    25621 tttgggattt atccagagga attagtcgta atctacgatg atttggatct ggcagtggga
    25681 aaaatccggc tccgtcaaaa gggaagtgcc ggcgggcata acggtatcaa aagtattatt
    25741 tcccatttga atacgaatgt ctttgatcga atcaaggtgg gaatcggcag gccagaaggg
    25801 aaaaaaacag tagtccagca tgtgcttagt cctttcagca aagaaaatca gcctttgata
    25861 gaagaaagca tgtgtcaatc tgttaaagca gtcgaatatc tcatagaagg gcattcgttc
    25921 gtggatgcga tgaatcgatt caattgagga aggaggaaaa ggaatggata ttattcaatt
    25981 agtcggaaaa gatccgcaag taaaagaatg ggtcagcaat ttagaagaaa aaatgcctag
    26041 acaactaatc acaggattag cgggctcagc gaaaacactt ctatttgctc gagcgtttaa
    26101 gaaaacaaat aaaaacatga ttattctgat gccaaatttg tattatgcga atcagttagc
    26161 agaagatctg caacatgtga ttccgcaaga tcagatccat ttatttccag tggatgaagt
    26221 gctttctgct gaaatggcct tttcttctcc agaagcgcga ggcgagagag ttgcaacgct
    26281 taacgcactt caagaaaaaa aagcaggtat ctatttgttg ccagtcgcat ctttgcacaa
    26341 acgactgccg aataagaaaa cttggctaca ggcgcaattg aattggcata ttggtgatga
    26401 gatagacgtg gaaaatcttc ctcgccaatt gactttgatg ggctatgtac gagaagagat
    26461 ggtcgcaaaa ccgggagaat tcagtattcg aggaagcatt atcgatattt acccgttaac
    26521 gacaacctat cctgttcgtg tagaactatt tgatgtagag atagattcta tgcggtattt
    26581 tgaagcagaa acacaacgct caatcgaaaa aacggaagaa atctgggttt ccccaacgag
    26641 tgaacaagtc tacggaccag aggatctaga acatggaata gagcaaatgc aaaaaatgct
    26701 agaaaaacgg cttgctgcga ccacagaaga agcagaccgc gagtttttgc aagattactt
    26761 cggtcagctg acttcaagct ggcaagcagg tgtcccgaca gaagaagcaa aattctactc
    26821 agatcttctt taccaagaat ctgtgacgat cttggattat ttcccttcaa atagtttact
    26881 agtcgttgat gattatcagc gaatcatgga aacaaatcgg gaaatcgaaa gggaagcggc
    26941 agaatggcag actcaaaaaa tcagcgaatt gcgggtgttt tctgagcaga cattttcagc
    27001 agatatacaa aaaatcgtgc agaaggaaaa gttcaccaca acctttttct cactcttcca
    27061 aaaagggatg ggaaatcttc gtttccaagc aatccataat tttcaatatc ggacgatgca
    27121 acagttcttc ggacaaatgc cattgctgaa aacagaaatg gaccgttggc gcaagcaaaa
    27181 acagactgtt gtgatcttta ttccgacaaa ggacagaata cgcaaagcag aacaaatgct
    27241 tcgagatgaa gacatattag tagtagaaac agaaaaaaat gcattgatca gaggacaggt
    27301 acaattagtt gaaggagctt tgcaggctgg ttttgaactt cctcaagaaa aaatagtagc
    27361 tatcacagaa aaagaaattt tccaaaaaac gacgaaaaaa caaacgagac gtcaaacagt
    27421 aacaaacgca gagcgattga aaagctataa tgagttaaaa gcaggagatt acgttgtcca
    27481 tgccaatcat ggtatcggga aatacattgg gatggaaacg ctagaagtag atggcgttca
    27541 ccaagactat atgactattt tgtatcaaaa cgacgacaaa ctgtttattc ctgttactca
    27601 actgaatctg atccaaaaat atgtcgcctc tgaagccaaa gcgcctcgca tcaataagct
    27661 aggcggaagt gaatggacga aaaccaagcg aaaagtctct tcaaaaatcg aggatatcgc
    27721 tgacgatcta atcaaattat atgccgcaag agagtcagaa aaaggatatg cttttggacc
    27781 tgatgacgcc taccaaaaag aattcgaaaa tgccttcccc tactctgaaa ctgatgacca
    27841 gttgagaagt gcagcggaga tcaaacggga tatggaaaaa gaaaaaccaa tggatcgact
    27901 actcgtaggt gatgttggtt atgggaaaac agaagtagcg cttcgagcgg cctttaaagc
    27961 gataaaggaa agcaaacaag tagccttttt agttccaaca acgatcttgg cacagcagca
    28021 ctatgaaacg atgctggaaa gatttgaagg tttccccgta aatatcgggc tattaagtcg
    28081 tttcagaaca agaaagcaac aaaaagaaac catcgaacaa ctgcgtacag gtcaagttga
    28141 tattgttgta ggaacccacc gaattttgtc aaaagatatt gaatttagcg atttgggtct
    28201 actgatcatt gacgaagaac aacgttttgg tgtaaaacat aaggaacgat tgaaacagtt
    28261 acgtgcacaa gtggatgttt tgactctgac tgcaacaccg attccgagaa ctttgcatat
    28321 gtcgatgtta ggcgtgcggg atctttcagt aatcgaaaca ccgcctgcga atcgttatcc
    28381 gatccaaacg tatgttatgg aaaaaaatcc tggggctatt cgcgaagcaa tccatcgaga
    28441 aatgggacga ggtggacaag tattctattt atataatcgg gtagaaacga tcgaacaaaa
    28501 agtagaagaa atccaagaac tcgttccgga agcacggatc ggttatgctc atgggcaaat
    28561 gacagaggcg caattagaaa atacgctttt tgaatttatt gaaggtcaat atgatgttct
    28621 tgtcactaca accatcattg aaacaggagt agatatcccg aacgcaaata cgttgttcgt
    28681 tgagaacgct gattatatgg gattatccac gttataccaa ttgcgagggc gggtaggaag
    28741 aagcagccgt gtcgcatatg cttactttat gtatgagcag cagaagatcc ttaacgaagt
    28801 cagtgaaaaa cgtcttcagg caatcaaaga ttttacagaa ctgggatctg gattcaagat
    28861 tgcgatgcgg gatctttcca ttcgcggtgc aggtaatctc ttaggtgctc agcagcatgg
    28921 atttattgat gcagtcggtt tcgatatgta ttctcaaatg ctaagcgaag ctgtttctcg
    28981 taaacaagga aagaatagtc aagtggagaa gaccactgtt gagatcgacc tcggtgtcga
    29041 tgcgtatcta cctgaaacat acgtggcaga tcaacggcag aaaatcgaga tctataaacg
    29101 aattcgtgaa cttgattcgc aagaaatgct agatgaacta gaagatgatc tgctcgaccg
    29161 ttttggagaa tatccagaag aagtagcaca tctcttgaca attgggcaga tcaaaatgga
    29221 tggcgatcga gcgctcctag aaacgataag gaaacaggag gcgcatatag tctttactct
    29281 aagtaaagtt ggaacaaaac gctatagtgt ggaacagtta ttcgaagcac tgacagcgac
    29341 aaaactaaaa gccagtctag gcgttgaaaa agaacagatg gtgatacgct tgacgatccc
    29401 caaccaaatg gaagaagcag tatggcttca ggaaatccaa aagtttgtca aagcgttgcg
    29461 tgagcaaaaa tatctgtcag cgtaggaagg agcaatgatt tggatcataa agaaatgcgg
    29521 cgcgtcatgc aaggagcatt tgtattgacg attgcttctt tcatagcaaa agtattaagt
    29581 gccctttacc gaattcccct ccaaaactta gtcggcgacg aaggttttta tgtttatcag
    29641 caagtttatc caatttatgg gatagcaatg actttagctt tatccggtct tccgcagttc
    29701 atatcaaagt acgttgcaga aagaaagaat cctttcgagc gaaagcaagc tttgcaaaat
    29761 ctttatccct tagtattttg gacaggtgtc tttttgtggt tgtttgtctt tgcatttagc
    29821 agctggattg cttggatgat gggagacata caattgaaac cgctgatcca agtagtttcc
    29881 tttacctttt tgctgatgcc tggtctatct ttttatcgag ggaatttcca aggaagattc
    29941 ttgatggaac caactgctgt ttctcaagta gttgagcagt tcgttcgtgt ttttgtgatt
    30001 gtctgtagtg cgtttgcttt tagaaagctt ggatggacgg tgtaccaaac agggacggca
    30061 gcaatgagtg gagctgtttt tggcggttta tgcgcatatg ggatccttca ctactatgaa
    30121 caaaagattc atggaggagc gttaagcttc cgacggtttc cattgttcaa acagccgtca
    30181 aagcccttga tccggcgttt tctggtagaa ggcggactgg tttcgattta tagtggattg
    30241 cttatctttt tccagctgat cgactctttc tttgtaaaaa atgctttgga agtttacgga
    30301 ctgtcagaat acggtgcgaa gatagcaaaa ggggtctatg atcgaggtca gcctttagta
    30361 caattaggtt tagtgattgc cactgcatta agtgcgacat tcctgcctgc gttgactcgt
    30421 catttgacca accggatcta tcgtcagttt ttacagacag ccaaaatcta tcttagattg
    30481 acgaccgctt tagcattagc tgcgtctttg ggattagcct tgctgctccc ttatatcaat
    30541 tatgcgttgt ttaaagatta tgcgggaaat gcagcgcttg tattatttgt tttttccatt
    30601 gctttcactg cagtcatcca agcttatcaa agtattgctc aaagcaagaa ctcgtttcgc
    30661 ccttctttaa aaggcgcagg atggggacta ttggtaaaag gattgactac ttcttttctg
    30721 accggtttat taggaacagc aggtgcaagc ctgtctaccc ttttaggatt aggtgtgacg
    30781 ctttggtatt ttgttcgaat tgaaacaaaa gagctaaatg ctttttggaa agaacggcat
    30841 tttggacgga agttgatgtt cagccttctt tgtatgacag tagtactgct gctgtattat
    30901 ggtgtattga atatagtatt tggtcctgta actcatcgtt ccactgcttt ttttgtcagc
    30961 gtgataggag ttgggatcgg ggcttctgtg ttcatttatg cagtgattcg tcttcgtctg
    31021 tttacagtac gagaatggct aatgttgcct tttggtaaga aaatcctgcg attaaataaa
    31081 aaaattaa
//
LOCUS       DASJOX010000021        30121 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJOX010000021 DASJOX010000000
VERSION     DASJOX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.021"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="U4A12_000912"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="U4A12_000912"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN1773319.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="U4A12_000913"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="U4A12_000913"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN1773320.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="U4A12_000914"
     CDS             complement(2303..2965)
                     /locus_tag="U4A12_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1773321.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="U4A12_000915"
     CDS             3149..4795
                     /locus_tag="U4A12_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1773322.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="U4A12_000916"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="U4A12_000916"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1773323.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="U4A12_000917"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="U4A12_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="U4A12_000918"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="U4A12_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="U4A12_000919"
     CDS             complement(8944..10023)
                     /locus_tag="U4A12_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1773324.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="U4A12_000920"
     CDS             complement(10126..10566)
                     /locus_tag="U4A12_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1773325.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="U4A12_000921"
     CDS             complement(10623..11033)
                     /locus_tag="U4A12_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1773326.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="U4A12_000922"
     CDS             complement(11213..11647)
                     /locus_tag="U4A12_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1773327.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="U4A12_000923"
     CDS             complement(11747..12595)
                     /locus_tag="U4A12_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1773328.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="U4A12_000924"
     CDS             12984..14750
                     /locus_tag="U4A12_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN1773329.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="U4A12_000925"
     CDS             14852..16111
                     /locus_tag="U4A12_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1773330.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="U4A12_000926"
     CDS             16258..17559
                     /locus_tag="U4A12_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773331.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="U4A12_000927"
     CDS             17562..18413
                     /locus_tag="U4A12_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773332.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="U4A12_000928"
     CDS             18426..19241
                     /locus_tag="U4A12_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN1773333.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="U4A12_000929"
     CDS             19392..20558
                     /locus_tag="U4A12_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1773334.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="U4A12_000930"
     CDS             20788..21435
                     /locus_tag="U4A12_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1773335.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="U4A12_000931"
     CDS             21519..22463
                     /locus_tag="U4A12_000931"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN1773336.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="U4A12_000932"
     CDS             22598..24049
                     /locus_tag="U4A12_000932"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN1773337.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="U4A12_000933"
     CDS             24070..24906
                     /locus_tag="U4A12_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1773338.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="U4A12_000934"
     CDS             24906..25565
                     /locus_tag="U4A12_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1773339.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="U4A12_000935"
     CDS             25691..26380
                     /locus_tag="U4A12_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773340.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="U4A12_000936"
     CDS             26386..27342
                     /locus_tag="U4A12_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN1773341.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="U4A12_000937"
     CDS             27386..27898
                     /locus_tag="U4A12_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773342.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="U4A12_000938"
     CDS             complement(28154..28702)
                     /locus_tag="U4A12_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1773343.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="U4A12_000939"
     CDS             complement(28791..29972)
                     /locus_tag="U4A12_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN1773344.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         9990 a         5457 c         5724 g         8950 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 g
//
LOCUS       DASJOX010000022        27943 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJOX010000022 DASJOX010000000
VERSION     DASJOX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.022"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1287
                     /locus_tag="U4A12_000940"
     CDS             <1..1287
                     /locus_tag="U4A12_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773345.1"
                     /translation="KNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILM
                     GCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLS
                     LLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTI
                     GLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWR
                     LNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTL
                     IAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELD
                     NSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKL
                     AIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1432..2172)
                     /gene="truA"
                     /locus_tag="U4A12_000941"
     CDS             complement(1432..2172)
                     /gene="truA"
                     /locus_tag="U4A12_000941"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1773346.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2363..2998)
                     /locus_tag="U4A12_000942"
     CDS             complement(2363..2998)
                     /locus_tag="U4A12_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1773347.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3145..4386)
                     /gene="sstT"
                     /locus_tag="U4A12_000943"
     CDS             complement(3145..4386)
                     /gene="sstT"
                     /locus_tag="U4A12_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN1773348.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4584..5582
                     /locus_tag="U4A12_000944"
     CDS             4584..5582
                     /locus_tag="U4A12_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773349.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5595..7613
                     /locus_tag="U4A12_000945"
     CDS             5595..7613
                     /locus_tag="U4A12_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN1773350.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7673..8389)
                     /locus_tag="U4A12_000946"
     CDS             complement(7673..8389)
                     /locus_tag="U4A12_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1773351.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8616..9158)
                     /locus_tag="U4A12_000947"
     CDS             complement(8616..9158)
                     /locus_tag="U4A12_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1773352.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9163..9390)
                     /locus_tag="U4A12_000948"
     CDS             complement(9163..9390)
                     /locus_tag="U4A12_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1773353.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9469..11349)
                     /locus_tag="U4A12_000949"
     CDS             complement(9469..11349)
                     /locus_tag="U4A12_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1773354.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11538..12152)
                     /locus_tag="U4A12_000950"
     CDS             complement(11538..12152)
                     /locus_tag="U4A12_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773355.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12294..12854
                     /locus_tag="U4A12_000951"
     CDS             12294..12854
                     /locus_tag="U4A12_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1773356.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13159..13473)
                     /locus_tag="U4A12_000952"
                     /pseudo
     CDS             complement(13159..13473)
                     /locus_tag="U4A12_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(13727..15362)
                     /locus_tag="U4A12_000953"
                     /pseudo
     CDS             complement(13727..15362)
                     /locus_tag="U4A12_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15917..16480)
                     /locus_tag="U4A12_000954"
     CDS             complement(15917..16480)
                     /locus_tag="U4A12_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1773357.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16495..17538)
                     /locus_tag="U4A12_000955"
     CDS             complement(16495..17538)
                     /locus_tag="U4A12_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773358.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(17671..18075)
                     /locus_tag="U4A12_000956"
     CDS             complement(17671..18075)
                     /locus_tag="U4A12_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1773359.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18308..18943)
                     /locus_tag="U4A12_000957"
     CDS             complement(18308..18943)
                     /locus_tag="U4A12_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1773360.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19209..20261)
                     /locus_tag="U4A12_000958"
     CDS             complement(19209..20261)
                     /locus_tag="U4A12_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1773361.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20274..21212)
                     /locus_tag="U4A12_000959"
     CDS             complement(20274..21212)
                     /locus_tag="U4A12_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1773362.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(21663..22313)
                     /locus_tag="U4A12_000960"
     CDS             complement(21663..22313)
                     /locus_tag="U4A12_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773363.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22413..22922)
                     /locus_tag="U4A12_000961"
     CDS             complement(22413..22922)
                     /locus_tag="U4A12_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEN1773364.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23123..23992)
                     /locus_tag="U4A12_000962"
     CDS             complement(23123..23992)
                     /locus_tag="U4A12_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1773365.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24012..25133)
                     /locus_tag="U4A12_000963"
     CDS             complement(24012..25133)
                     /locus_tag="U4A12_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1773366.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25135..25530)
                     /locus_tag="U4A12_000964"
     CDS             complement(25135..25530)
                     /locus_tag="U4A12_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1773367.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25549..26355)
                     /locus_tag="U4A12_000965"
     CDS             complement(25549..26355)
                     /locus_tag="U4A12_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1773368.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26348..27136)
                     /locus_tag="U4A12_000966"
     CDS             complement(26348..27136)
                     /locus_tag="U4A12_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1773369.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27150..27626)
                     /locus_tag="U4A12_000967"
     CDS             complement(27150..27626)
                     /locus_tag="U4A12_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1773370.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(27698..>27943)
                     /locus_tag="U4A12_000968"
     CDS             complement(27698..>27943)
                     /locus_tag="U4A12_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1773371.1"
                     /translation="DLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEF
                     QKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         8786 a         5637 c         4318 g         9202 t
ORIGIN      
        1 aaaaacaatc gttatatttc gctgttattt tcgattattt atacattttc cgcctatcgt
       61 gccattgaca tattccatcg tgcttcattg ggagaagctg tcgctttaac atttttgcct
      121 ttgatactaa tggggtgtta cgaaatttat attagagatt accaaaaatg gtactggttg
      181 tctattggca tgacgctagt agtgtatact catttacttt ctgtcgctat ggtttctgta
      241 tttattggag gaacgttgtt tctttctttt tatttttggg accaaaaaat cgctagactc
      301 ttaagtcttt taaaagctac cgtcctcacc ttttttttaa gtgcgggatt cttaattcct
      361 tttattcaac aatctagagc acaagaattg aaagtcccac tgggaaaaga attaagtggt
      421 atggctccta gtgatatgct gacacatatt ttaaataata actataacaa ctatactatt
      481 ggattattct tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct
      541 gatgatttat ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt
      601 ccttggcaac tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat
      661 ggtttttctt ctttatttat agcctatacg atgagtatag tgatctatta tattttttct
      721 actaaaaata attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc
      781 tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa
      841 gacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc
      901 tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca
      961 acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa taataaaaat
     1021 actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat taatggcaaa
     1081 ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc tccaggtata
     1141 aataaagtcg ttattactta tcagtacaca aaactagcaa ttgcttcaag atatttatct
     1201 atcgttactt tgattttatt tttactttat cgatttactt tttctaaaca aaaacaaccg
     1261 agaagagaag tacaacattc tcactagatt tttatttaag aaagactttt tagctgtaca
     1321 cataaacagg ctaaaaagtc tttttttata tttctttaat ttgcttcata cattatttaa
     1381 catcgatatt tatactgtga aacatgcgtt tcatccatta tcaaagtatt tttatcgata
     1441 ataaacttca tccaaaaaca gaccttgtgc agggacggtt tctcctgcat gttgccgcac
     1501 tttgctttca aatatttcat cgattgtttc tatcggcatg gcacctgtgc cgatttctag
     1561 taatgtcccc atgataatcc gcaccatttt atataagaat ccgtctccga caaaagtaaa
     1621 atgaagcatt tctccctttc gttcgataga taattcatca atcgtacgca ctgtggattt
     1681 ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta atttttcgca
     1741 agctgcattc agcttgtcca tatccaactc ttgagggaaa taaaagctgt ggtttcgctc
     1801 aaatgctgaa ggaatcacgt tgttccaaac ataatagctg tattttttgc ccaccgcatt
     1861 atatcttgaa tggaagcgct caggcatttc ttcgattctt ttgatgacaa tatcccgcgg
     1921 aaggtaacga ttcataaagt cgatcatttc tgccagtcca atttctgtgt ttgttttaaa
     1981 atttgctact tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc caataatttc
     2041 tatctcttca tttgtcatct gatgcaatac tttttcgatt tttccttgaa tggttttatc
     2101 gtcatctcca agtctttgcc atcctgaata tctttttccg tcatattcga ttgtcaactt
     2161 gatgtttctc atattttagt ggctcctctg tcttccattt ggtcttctct ctaccttttt
     2221 aagattgtag cacaaaatat agctccttgc ttctctaaga cagaagctat acaagaaaac
     2281 tgtggaaata tatatttcag aaaaacaaaa aacagccacc ggatgaagta tccgctgact
     2341 gttcaatttg acggctcaag ttttactgtt tgagatgcaa gatactcaac ctggcaatgg
     2401 gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc agtaatggcc
     2461 aagaatttat agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc aaactgatca
     2521 cagacagtcc aaggtataag tggcttacgt ccatttgccc attttttcga tagatcaaga
     2581 aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa acctgatccg atattatgga
     2641 cccatgtcga gacattccac gaagattcat gggtattgat actgaacaaa cctgtaaaga
     2701 tacaatcacc tataccatag aacgcgatgg ctgctgtaac tagggtagct aacacacgag
     2761 atgtactaac aaatgcagca tacaatgcag gcaatgacag gatgaaaaag atccctgaaa
     2821 ccacagacca aataagaaac gcttttctta caggactgga aacctctcca aatacactga
     2881 tcacgctctt catttgatct aattcaggat aatacaatcc taatacgtac ggagtcaaaa
     2941 aatcactcag cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga
     3001 tacgcctcca ataacaatta cctatgcttt taattgttat tataaacgac tttatcttga
     3061 tttaacagaa acctctattt acacgaacat ctatataact cattaaaaac tcgaatcatt
     3121 ttaaaaatac taaatatcta acgttcaacg ttcacttaat ccatctaaac aatctttcaa
     3181 tttgattttt tccccacttc ttttacgcgc gcctagctct cctattgcag taaacaacag
     3241 atcacttgac gaatttaagg cagtttccat tgaatcctgg ataacaccga taataaatcc
     3301 aataccgacg atttgcatcg cgacatcatt agagatacca aacaaactac atgctaatgg
     3361 aatcaataac agtgaaccgc ccgcaacacc tgaagctcca caagcagcta aagcagacaa
     3421 aatacataaa aggactttca aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt
     3481 caacgtcatc aaggaaatgg taatcgcggc accgcccata ttgatcgtag cacctaacgg
     3541 gatcgatatg gaataagact cttttgtcaa tttcaatctt tcagccaaca tcatgtttat
     3601 cgggatattt gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca
     3661 aaagaaaatc aatggatagg gattttcttt aataaacaaa aacgtgagga atgggtacac
     3721 aactaatgcg acaaacagca tcgtaccgat caaaacaagt aaaagctgca gatactctgc
     3781 caatccagcg attccagtcg tagcaattga ttgataaacc aatcctaaaa tccctatcgg
     3841 agcaaatgca ataatcactt gtacgacttg tgacaacgct atagatatct gatccaccaa
     3901 ctcttttgtc gattctgaac gtgctcgaaa agctagtccc aacaatacag cccagaataa
     3961 gacgccaagg taattcgcat caataagaga ttgaacagga ttttgtgtga cattcgtcaa
     4021 catagtagat aaaacacttt ccaaactttg cggtgctttt tcagatactg tttcttgtaa
     4081 agtaatagga acagtaaaaa ttgaacttac aataactgct acaagtgcag aaagcaatgt
     4141 tcccactaaa tataagatca aaataggctt cacgaatgtc ttattcccaa ttttatgctt
     4201 ggcaattgac gccatcgtca agaaaaaaac cagaataggt gcgattgcct tcaagcaact
     4261 gacaaacaac gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa
     4321 tattccgact aaaataccaa caacaatacg ttgaactaac gacatatcca aaaacttctc
     4381 taacaaatga ccaccctttc acaattttaa caatcattgt atcattttat gcaaagaaac
     4441 taaaggtata attttctaaa aattctaaca atacaaacaa ccatttcttg ctaattttgt
     4501 actttttctg tctacacgtt gttttttgag atattctaag ttaggatgtt attaatgggc
     4561 aacttatcag gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg tgtttattct
     4621 tgaaaaaaat aaagaaggag agttatggct tcctgacaat ctttgtcgct tgtataatga
     4681 tggttttaca gcgtatattg atttcagcag tcactattcc tacatatgca acaaaccctg
     4741 gtcgattcta catctataca tttttacaaa cttccgttgt tattggtgca aatttgatcc
     4801 cactttacat gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa
     4861 gtcgttttgc ctttatattc ctaatagcca cactagtttg caatatcttt ttttacgtac
     4921 aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc agtcagaatt
     4981 actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat acagttggat
     5041 ttttaaataa attatcgaaa acaacaatta aatatctact attattttta acaattggat
     5101 tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat ggacagagta
     5161 ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg aactggaaag
     5221 aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta ttcggtttga
     5281 ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca gcgctgaggt
     5341 tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt ttatttttag
     5401 aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct gtatttttga
     5461 ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct acatttatga
     5521 aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag ttttttattt
     5581 gatatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa aaaatccgtt
     5641 tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt
     5701 ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt
     5761 tttactgctt taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa
     5821 ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca
     5881 tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct
     5941 tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa
     6001 tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct gaccggattg tcagaactat
     6061 tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga
     6121 ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga
     6181 aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta
     6241 atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct
     6301 ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg
     6361 acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga
     6421 aaaagtacaa cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc
     6481 agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga
     6541 cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat
     6601 tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag
     6661 gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc
     6721 gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt
     6781 ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct
     6841 attatttaga cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca
     6901 tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acggaaacgt
     6961 cacaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg
     7021 aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa
     7081 cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg
     7141 acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata
     7201 gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata
     7261 acaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata
     7321 actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt
     7381 tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta
     7441 atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa
     7501 atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg
     7561 ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aataaaagac taacaaataa
     7621 actgagcaag gggttgtgat aaaagacatg tcacaacccc ttgtttatct cattaatttg
     7681 cttgtccata aacgatttga ttaaagccat ttttcaattc attattcgct gtctcgttga
     7741 agtaaagata attatcggga ttcccctgat tttgttgtgt caccaatact tttggctgat
     7801 tttgctgaaa cccgaataga tagacgtgct ttttcagata tggttctgtc tctgcatcgg
     7861 aatagacggc tgtgatttga tagtctgctt gacctgtccc actttctgac cattctgcag
     7921 aaacaggcac tccaccaata gccatcttcc actccccgtt caaaatagct tgagggactt
     7981 taagaccata taagtccaca ctcatttgat tcgtataact tttgtattgt tgtcccattg
     8041 tttggcccca tttggtcata aatgcctcta attgtgcgga tttgtctgca ttccacagtg
     8101 tttgttcaac ttgttcgctg ctttctgaag aggaagatga agcctctaca gaagaacttg
     8161 aagtagatgt ttgagttgtt tcagtcgttt tggaagactc tgacgaagta gaggtatctg
     8221 ctgtttctgt acttgctgca gttgacttgc tctctgtgct agttgttgta gtagttgcaa
     8281 ctgttgcttt cgctgtttct tttgttgatt cttttttcga ttcattcccg ttggtacaac
     8341 cagctaacaa aaaagctaga ccaattgtta ctaaaatcct ttttttcaat taaaaaactc
     8401 ctttcgatta ctcccacttc tttattctat caaataagca atgtgatttt attgattttt
     8461 cgtattttct catcaatctt tcaaatcttc taaaaactga gaagcacccg ctgatgttgt
     8521 ctgtaataca tcagcgggtg cttcttttat tttaatcttc gtgattttcc aatttacctc
     8581 acgctttata tttgtatttg aagaaacata gacttttagt catcatcgtc ttcctcatcc
     8641 aaatcttgtc ggacagtttt tccttggagt ctttgtactt ggtagatgat ttctttattg
     8701 aaacttaaaa gttcaacaag caattgttgc aagccatatt tttcttcttt ttcagctaat
     8761 ttgattgccc gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa gttttccaac
     8821 attgttgagg ctggtaaata tttgttttga ccatgttcgt aaatttttcc ataacgcatg
     8881 acttcttcta aagtagacgg aacgatttca ttttcaatca ctaactcatg agagatttta
     8941 ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa
     9001 gatgtaccca aaacatacaa ttgtgcttgg atcaatttca tttcttctaa tttaaaattt
     9061 gctaatacat gccccaccat ggcgccagca gttggtttat gatggtcgat atctgcttgt
     9121 tttgtttctt gaacaaattt ttcttccact atattcattg atttacgcct cttttacttt
     9181 tgctgattgg acttcataac ctaatttttc cacaactgca gctaattgtt cagggtggac
     9241 ttcatctgga tcaaagtttg ctttgatttt gctcgcatta aacaaaactt tcgtattgct
     9301 taccccaggc tgttgagaaa ccgcatgttc gattttttgc atacagcttg gacaagctag
     9361 tgtatctaat tgcataataa tttttttcat cgtagttctt ccttcctttt tcttgatagt
     9421 cttattctag attacagctt ccataaaaaa attgattctt atcaagtttt acgatttttc
     9481 ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa cgatcaagat
     9541 actagcttcg tgaacgaaca tcccactagc catgtaaata aacccggcaa ataagccgtt
     9601 gagtaaaaat aagacgacac caatggcaat cactacattt tcacgggtat ttaaaacagt
     9661 tttcttagcc aagcggtagg catgaaccaa cgattcaaaa ttagattgca tcaagacaac
     9721 gtcactcgtt tcgatagcaa catccgttcc tgatcccata gcaatcccga tatcagccgc
     9781 agccaaagag ggagaatcat tgattccatc tccaataaaa gcaacgcgca atccttgttc
     9841 ttggtatttt ttcacaaatt gaaccttttc gtctggtaat aattcagcat ggacttcatc
     9901 aatgcctaac atatcagcta cataatcagc agtcatctga ttatcacctg tcagcatcac
     9961 taactgtttt gctcctgctt gtttcaactt agctaattgt tcagcgactt ctggtcgaat
    10021 cacatctgac acacctatga tttgagtgat ttttttgtcg attgctacaa taatgaccga
    10081 gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt
    10141 gtgagcggca atcaatttgg tgttgcccgc acaaactgta tgaccatcaa tctcagctgt
    10201 gattccttgt cccttgattg tctgattatc tttgactgct aattgttgaa aatcaatgtc
    10261 tttctcctga gcataatgga tcacggcgcg ccccagagga tgatcggata acgtttctaa
    10321 tcgagccact aaagctaagc tgtccccttc atcctctgta tagttcttga tagtggatac
    10381 agcagtagtt cctttggtca atgtaccagt tttatcaaag acaaaagtat cgattttgga
    10441 aaaagtatcc attgcttcac cgcctttgac taagatgccc cgtttcgctc cattaccgat
    10501 tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac caagaaccaa
    10561 aacagtgatt gctaaacgaa aatcacgact aatcagacca acaaccaacg caataagtaa
    10621 aacggttgga gtataatact gagaaaatcg atcaatgaat ttttccgcat gggatttggt
    10681 atcttgtgct tcttctacta attcgataat tttggcaaag gtcgtatcat ctccgacctt
    10741 agttgcttca atcgtcacgt agccatctgc taaaatcgtt ccagagaaga cttcatctcc
    10801 actgattttg acgacttgtc gtgattcacc agtcactgcg gcttcatccg catacccttt
    10861 cccgtcaatg actcgtccgt ccacaggaat agctccacca gtttttacta agacgatatc
    10921 gccttcttcc acgtcatcga tttccacttc ttcagtcgtt ccatcttctt ggacaacact
    10981 agcagtagat ggggccatct cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt
    11041 tttttgttcc agatagctac caaacaaaaa caagaatgta acgatagctg actccttgta
    11101 ttctccgatc ataaaggcac caagcacagc aatagtaacg agtaaatcaa ttgagataac
    11161 tttgactttc actgcctgat acgcatggat cataataggt atgaccgcaa taatagaagc
    11221 tatggctaga gcaatgttat agaacaagac aaaaccagta aattttccga taaaaccaaa
    11281 aataatcaac acagctgata aggcggtgat atgatttcga tgctcttgga tccacttttg
    11341 taatttcatc tttctccctc ttttcctttc attcttacga gactaagtat agatacttat
    11401 ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga
    11461 atacactaca aaaaatagtt agcaagaaga ctcactaaaa gtgatcttgc caactattgt
    11521 cgtttctatc gaaattttca ttgaatgatt ttcctgtaca gcatacatcg gagatctatt
    11581 tctttatcta agatttttat cttgctaatt ttttccttta gtctttcaaa tccacgtcgt
    11641 tcatagaatt gatacgtaca gccactatct gtaaaaagga atatttcttt tcctttctct
    11701 cttttttcaa attctgttag caacttggta ccgatgcctt ttgttttcaa atcaggattt
    11761 gcagctaaaa aaatgatctc tccatctggc gaatgcttct ttaaatatgc tgcaagcatt
    11821 tgttcatttg ctgtctcgta agctccttta ctttctttag caaatatatt ttgtaaaaaa
    11881 tcgaatatct taacatatat ttgctgtcca aaagaacgat gtttcttttt ttctcctttt
    11941 atctccgcca gcaaaacacc tgccaactcg tcctctgcat acaaagcaag aatttgagtt
    12001 gctcgattca actccatata caaaaaatat cttccgtaaa gattcagcag aagttcattt
    12061 tccatatatc tctcaaagtt cattccttca acagcaaaat agatagcttt tttgtaatct
    12121 ttcttttcta agggtttaaa agttagtttc atcatttcgc ctcctcaact tcaatgattg
    12181 tgttatactt acattataat agacacagtg tctactatct ataatcaatc atctataagt
    12241 ttgtcgatta agcgacagct ttgctttgat atgtcggata aggagatagg taaatgaaaa
    12301 agacagatag acgcgtcaaa aaaacagaaa aagctttggc tgaaacacta tccacattat
    12361 tagtaaataa aaagattcaa gcaatcacta tacgcgaact aacagaaacg gcagatgtac
    12421 atcgttcgac tttttataca cattataagg atatctatga tctatatgat caattagaaa
    12481 gcaacttttt tcgggatcta aatgaaattc tctcttatga tccagcccat agctatgagg
    12541 aactatacac tcgcttgatt gactatgttt acgcaaatac ttttttatat cgcttattta
    12601 tcctcaacca tggtgatatt tcgtttcaga agaaagttaa acaagttatt gaagaaaact
    12661 atctgaacat atggctatat gaagacaaga aaacgattat cacagaagaa atgagattct
    12721 ttactacgta ccacgtccaa ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt
    12781 acaagtactc caaaaaagaa ttattagaac tcttacagaa actgaatgat cacatagaga
    12841 aaatcatgcc ttgaataaaa acatactaag cttagttaag aagaaccgta accttctcgt
    12901 ataacaactg aatcttattt agccaaattg tttactgaag attttttgag taaaatttct
    12961 ataattccta cttattgaaa agtagtttgt agtgctgctt ctccctctct aagtgatata
    13021 tcttgttgta ccaatcaaat tcacctcatc attaataaat tacttttagg ctaatttatt
    13081 acaacttata ggttgaagct gtaaccaatt tatagtcata ttatattttt atcatccagt
    13141 ttattgaatt cttctcgttt acctcaattc attttcaagg ggaatcagct catcatctaa
    13201 cattttacgc catgctcgct tcatttcttg tgggtattta aatggtttcg cttctggatg
    13261 tttggcttta tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg
    13321 atcttcacct ggcatatatt gcagattaga agcatgtaat tttttctcag aaacaaacca
    13381 ctcattggaa aagacatgaa tcaaatcatc acttacttgt gtaaaatagc gacgtttaat
    13441 gacaaagata tcttcattgc tatctagtgt accttcattt ctttgtgctt aaattcaaag
    13501 ataattttct caataaagat ttttgattcc agtaagattc ctttactcct tttacaacat
    13561 agcgttgtcc tttgttttga aacatcttct tcccctcttt gccattaaaa atagcccaat
    13621 tctctataaa gaagattaag cttcattatt ataatataaa gtttaaattt ggtttattac
    13681 ttactttttc ttattctcta gtaagctaat attgaaaaaa atgtaatcag atcattattt
    13741 ttatatgttg taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac
    13801 taattaaaaa agctaacttc atagttggag gataaatgga agaaacttca atgattgttt
    13861 ctccttgtgt tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt
    13921 acttttgcga ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat
    13981 tcatgtactg tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc
    14041 ttgccaattc ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt
    14101 gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc
    14161 attaccaagt gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca
    14221 gatacagcca acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct
    14281 gatgtgctct tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct
    14341 aagattcgcc aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga
    14401 aaaaatttgg tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg
    14461 cgattaaatc ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct
    14521 accgtaaagg ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat
    14581 gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg
    14641 aacatcggat accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac
    14701 tccacaatca accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag
    14761 actagtaagc caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt
    14821 aagctcatac tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa
    14881 gcataataca taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga
    14941 tgataaatat caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc
    15001 gcatcgattc tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca
    15061 ttcaatagcc aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa
    15121 atgactgccg gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga
    15181 ttaaacgtcg taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca
    15241 aaatggaagg caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt
    15301 gaataaagtc cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc
    15361 attacttaat tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa
    15421 taaatcgtta tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg
    15481 taatggtctg aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag
    15541 cttaattatg tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa
    15601 catctttcat aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact
    15661 ttactatctt tcattcttct tgtctcactt atgataaggc gctgcgtatc atgagtgaat
    15721 gatattagga aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt
    15781 catgttagaa gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa
    15841 atcaattaaa ttccataaaa tataaataat agacctagca tcatctggta taaaatggat
    15901 gctaggtccg ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta
    15961 ttttagtagt catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata
    16021 aatttgtacc atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc
    16081 aatataatgg cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc
    16141 taattcgaat caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac
    16201 tttttcccat agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat
    16261 caactagttt agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct
    16321 cttgtatatc tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat
    16381 agccatattg aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag
    16441 atgatttttc ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt
    16501 tttagttgca gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag
    16561 taataaaaca ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc
    16621 aaacaatact gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt
    16681 gattcgtttt aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata
    16741 aggaccataa gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt
    16801 atcagatgtt tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc
    16861 tgttttcatt tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag
    16921 tgtgaagttg ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat
    16981 ctctgagaga tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc
    17041 tgctaagata tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt
    17101 caaccataaa agttcccgtt ttgtatcata tactaagccc cctacatgtg gccgtttagg
    17161 taaaatcagc agtttgatcg gctgtcctgt ttctctatcc agtacatgaa taatagaatg
    17221 atgctgatgg tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc
    17281 ttgcggcgtc atccattgac aaacctcatt ttcgccggtt tccggcacca ccattcttgt
    17341 ttctgttaat ccaggaatgt tatattgttt tgcttcagcc ttttcccaag ctgcttgaac
    17401 aataggatac cgttttaact ttttttcaat atcctgatgt atccgttgct gcttccaatg
    17461 cttaaacttt aggacatagt aaacaagaag gccaatgacc agcaacacta aaatcattat
    17521 tgctacccgc tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg tccatatacc
    17581 gctattaatt accgtaacta catgtttctt ttactagcgt ttctttatag gatctttcat
    17641 aatctcattt atagtaacat taagaatccc ttattctact atagtcactt ccacatcaat
    17701 attgcctctt gttgctttag agtatgggca aaatgcgtgg gcttcttctg cgattttctc
    17761 tgcttggtcc ttctcaagtc ctggcaaaga aacttctaaa acgaccccga tttggtagcc
    17821 cccatcttct aattgaaata aagatacccg tgctttgact tcagaatcag actcaagatt
    17881 attttcttcc agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa
    17941 aagttgttcc ggatttgtcc cttcactatg gattcctggg cggtcgatct tcatgtgcat
    18001 acttccgttt ggtgcctcaa cttcgccatc acggcctcca cgattgatca ttgctgtttc
    18061 gtacattttt ttcattgaga tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt
    18121 attttttaat ctgacaaagg taatgcttgc attcggcgac atcagttaga caacatcgat
    18181 gatcagtgat agaagcagtt tatctataca aaaaaagaag ggttctatga tatttggtgt
    18241 ccttactcgc cttgagtaac aacaccaaat atcatagaac cacttaatca gcttcacact
    18301 gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga
    18361 aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg
    18421 gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat
    18481 aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga
    18541 aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat
    18601 tacaagagct ttcattcgta ttgatactga ataagccagt aaaaatacaa tcacctatcc
    18661 catataaacc aactgttgca acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata
    18721 ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca
    18781 agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt
    18841 gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca ctcatcacac
    18901 ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt
    18961 tagtgattat tcgatacatc caacaatcaa attttttctc attttaacac atatttttat
    19021 tctagttgtc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa
    19081 tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa
    19141 acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt
    19201 aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc
    19261 tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca
    19321 acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg
    19381 ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta
    19441 atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc
    19501 atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa
    19561 gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa
    19621 acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa
    19681 aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta
    19741 ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca
    19801 agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa
    19861 ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata
    19921 atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg
    19981 actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat
    20041 ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact
    20101 gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct
    20161 aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta
    20221 ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt
    20281 acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc
    20341 gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt
    20401 aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc
    20461 gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct
    20521 actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat
    20581 gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg
    20641 ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat
    20701 cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa
    20761 ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct
    20821 gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata
    20881 tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt gattaatgat
    20941 tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca
    21001 acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct
    21061 ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag
    21121 ccatcgatta ataatacttc gttcccactt tggtgaagca tcaacccaaa gcgacttccc
    21181 attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta
    21241 tactcatttg agaatctttt cacaacctcg ttttgttatt cttctcttat ttatcatgtt
    21301 tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa
    21361 tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaatc
    21421 tcactcctct gattataaaa gagggggcat taacctcgtg tatactaaaa ccttccattc
    21481 attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac
    21541 acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact
    21601 tctttcccag attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc
    21661 ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg
    21721 cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag
    21781 tctaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc
    21841 cttaaaatta tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct
    21901 ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct
    21961 ctatgtgtta catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct
    22021 tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt
    22081 tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc
    22141 caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac
    22201 tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc
    22261 tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca
    22321 atccagctcc tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa
    22381 caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct
    22441 aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct
    22501 tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa
    22561 acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca
    22621 ttgttgatat atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt
    22681 tgcggaccac gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg
    22741 atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc
    22801 agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc
    22861 aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc
    22921 atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat
    22981 ttttaggatc aatgataaaa accgcatcac ttatgataag gcgctgggta tcatgagtga
    23041 atgatattgc tgaatacaaa tagtccgacc acattaagtt gatcggactt cataagcatt
    23101 ttattgacta atactttttt gcttaagaat tgattctaac atcttttgct aaagttaagc
    23161 atcctatagt tcctgcatta tctgctaact taggtgtgac aatgtattct tcgatatttg
    23221 gcgtatcgac atagccgtta attagttcta aaaaacgtct ttgtactttc tttttcagat
    23281 gttcttgttg catgacacca ccaccaaaaa ttataatatc tggtgaaagc atcagggtcg
    23341 tattacaggc acattgtgca atataatccg cctcgatttc ccagaacgga tcttctatag
    23401 aaagatcttg tcccttggtt cccgtacgca tctcgatagc gggtccagat gccaaccctt
    23461 cgagacaatc atgatgaaaa ggacaagtac cagagaaagt atctcctgaa tgtctcttaa
    23521 ctaacgtatg ccccatttct ggatggctaa acccttcaac aaaaatccca tcttgtatag
    23581 ctcctccacc aattccagtt ccaacagtgt aataaacaac actggataaa ccttttccat
    23641 gacctgaaac atactccccg taacaagcag cattcacatc ggttgtccag gcaattggaa
    23701 tttcgaaatg cttattcaac gttccaacaa aatcaaaatt tttccaggca gtttttggag
    23761 tagaggtgat atgtccatag gtctttgatt cacgatggat atcaatcggt ccaaatgacc
    23821 ctactccaat gctttctaat tgttctttat agtgattaaa gaaatcaatt accaaactca
    23881 ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc taaaaccttt aaatcatcat
    23941 tgccaatggc acaaacaaat ttagtcccac ctgcttctat tgagccatac atttcttact
    24001 ccaccgtcct tttatctagg aattcgttct tccctgtcta atagtttttc gaaaaactca
    24061 tgtggttcct tttggtacca ataggcaaca gaagaaatat cgtcttgacg ctcataaagc
    24121 ttgatgtcat catttcctag ggtttggaaa gtaacacgta aattttcttt gaaactaatg
    24181 gggtcgggta aatgccagcg gtacaaccca tgcatcggta aagcatcatc gccaaaacca
    24241 tgaactggat ttggattagg ttttccagca ctaaaataat cccgggtgtg atctctttta
    24301 gattgaaaag gatagcccat aaatagcgta ttaaaatttt gagcatatgc cctttctcca
    24361 aagttctttt tatggaaagc ccaagcgccg ccaaaataat cttctgctcc tgtagatgtt
    24421 attgtaggga aatcttcatc tccatctata taaaacttaa attctccttc cccccaccag
    24481 tatctttcca atgcgcacaa agcaaagtag gttcctacat aatacccagt gcctttgatt
    24541 ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt ccatttagca
    24601 tgaaaataca tcgcatcatc cggtacttca gtcaatgcat aattaaatgt ataaaaaatg
    24661 tgcttaataa actcaggatg ttcattcgtt aaagtaatct tagctttttt tcgaaatggc
    24721 atctcaaaat agcaattcat ccctgatgtt ggattcacta cgattggtaa ggaattcact
    24781 tcgcatcttg tcccaaaccc attacaaaaa aaatctccta atggactttc tacagaagga
    24841 gtggattcat catcccaata aatacgcaaa attaaatcac gtaagacgaa actcccagcg
    24901 attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc ttccatttct
    24961 gctaaagtaa ttgtctctcc agcatttatc ggaacagaag gtgacccttt tctcccaatt
    25021 cctagatgac tcgctgtcat tgccgctttc cctttttcac catttttatt ttctggtgaa
    25081 atagttctac tttcctctcc cttgtggagc catatattat ttaaacaatt cattttattc
    25141 gtcctctatc ctttcttcac ttttttgatt attcatatag atgatttgtt gttgtgcctc
    25201 cgcaataatt gctccaatat ctaaattatc atttcctaac tgcaaagcga ttgacattgc
    25261 caagggaaga ttcattcctg caattacgtg cactctgtca gaaatatagg taaaaaactt
    25321 ttgatttaca cttcctccag ccatgtcagt taaaacaatt atttcgtctg tagtatccac
    25381 taactcttta atttgttcat ctatatcatc aatttcttcg acatatgcac ataagctact
    25441 aatttgatta tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct cagctagtat
    25501 tgaatggcta gcaatgatca atcttctcat aattcaaatc cttttccttt acgctaatat
    25561 tccaaatgca gccataacca tcgcaaaaac aataacgagt agaatcaaaa tggtcatact
    25621 gatttttttc ttactgagta gataatatgc tgcagcagta ataattactg gaattaatgt
    25681 tggtaaaatt ttgtccaaca ttggttggat tttcatacta acttcaccaa aagtgaattt
    25741 aagtggcgtt gaaacactga taactgaagc aattaatgat ccaactactg ttagccccaa
    25801 tactgaagca gcttcagtta aataattaat cttttcaccg tattcagtaa caatcttcat
    25861 tccctgctta taaccaaatt caaagcctct cattcggaaa aagaagatta ccatgattaa
    25921 aatgaaccat aaaaagattc ctacaggatt attttctaaa gccatataag ccgcaattga
    25981 tcccatgatg gttgggatca tcgtcatcaa aagagagtcc ccaataccag caattggacc
    26041 catcgtacca actttaaagt cattaatcgc atcttttgct tctaatcctt gcttttcctc
    26101 cattgcaaca caagcaccta aaataatggc tgccagccaa ggctgtgtat tgtaatactg
    26161 aaagtgatta tttaaagctt cgatgtattc atcatcatct ttgtataatt ttcttaaaat
    26221 aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa aggtacaaac
    26281 tgacatcaac caacgatagt tcgctttcct aagatccttt ttcgttactt gatatttttc
    26341 gctcatttta ttcatcctcc ccaataaacc caacatcaga agctcccgca gaagcagtga
    26401 tttgtgcatc tttttcagct ctatgataca cattaaatgc taaagcgaat cctatgatcg
    26461 ccgctccaat aataggtacc ttcaaaaaag cggctaagaa aaatccaaca agtaaataac
    26521 taatgtatcg acctgtaggc ataaagcgta acaacatcgc aataccaaca gcaggcaaca
    26581 tttttccggc taatgttagc ccatccataa accaattagg taaaacatcc aaaacgtaat
    26641 taacagcaga ctgtcctaaa gtaacggcaa taaatactgg aattgctgca gataaaccaa
    26701 agaaaattgg ccccaaccac aaaacactat tcattttctt aaattctcgt ttattcgcat
    26761 attcctgtga tttgcgaaca acaaaaccat ttaagatttt gacaattaca tctagttgca
    26821 cccctaacat acctacaggt agtcctattg ctaatccggc ttctactcca agctggctac
    26881 caaatgccgt tgcaataatt gcgcacgttc catagtccgg tacagatgag ccaccaaaac
    26941 cggcaactcc taatgacatc aattgaactg taccgccaac tatcaaacct gtttttatat
    27001 cacccatcaa aattcctgtg aataatcccc aaaaaactgc gtaatttaca aagatcatcg
    27061 gaatcccata ataatcaaca tgtttaataa atgctaatat agtcagcata ataatttgaa
    27121 aaatagtaaa atgcattatc cttactcctt tatcctaatt ttgattgaat ttcctgtgcc
    27181 ttatccgccg gaacaaattg tgtaacaatt tctacaccag aatccaacaa ttttttatac
    27241 gtgctgatat tttcatctga tgcataggct ctagaagata gctgtaatgc attatcttta
    27301 acaacagtac cgccaacaat tagtcggtca attctaatac cgttatctac taaatgaagg
    27361 attgtttccg gctctttcac gatgacaaat actttttcct gatcgtattt ccccttttta
    27421 aaattcgcaa tcgctgtttc ttcagtgata attgatacag ccattccagc aggtctagct
    27481 aaacgcatac ttgatttttg gatgtcgtta tttgcgacag aatcatcgat caccatcact
    27541 ctgtttgctt tagacattgg tgcccattgc gtgacaataa tcccatgcaa taaacggtta
    27601 tcaactctcg acataacaat actcataaat acttcctcct ttatctttca ttttcttaca
    27661 agcaattacc atgccaaaac aaaaagaact tgataaatca agctcttttt acataatcat
    27721 aaacgtattt aacctcatga atcgggatgt cgacactata ataatgttca acgacactga
    27781 aagattgctt aaaatgtgtc tgaaattttt gaaattcctc actcgtttca ttgaaataaa
    27841 tttcatcttc caaagtattt tgttttacta atcgttcaat taagcaacta atgtgaacat
    27901 ataatccgaa acacgtatta ttatcaaatg aaattcctag atc
//
LOCUS       DASJOX010000023        27887 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJOX010000023 DASJOX010000000
VERSION     DASJOX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.023"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(279..1223)
                     /locus_tag="U4A12_000969"
     CDS             complement(279..1223)
                     /locus_tag="U4A12_000969"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1773372.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1519..2463)
                     /locus_tag="U4A12_000970"
     CDS             complement(1519..2463)
                     /locus_tag="U4A12_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773373.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2684..3898
                     /locus_tag="U4A12_000971"
     CDS             2684..3898
                     /locus_tag="U4A12_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1773374.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3920..5935
                     /locus_tag="U4A12_000972"
     CDS             3920..5935
                     /locus_tag="U4A12_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN1773375.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6018..7391)
                     /locus_tag="U4A12_000973"
     CDS             complement(6018..7391)
                     /locus_tag="U4A12_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1773376.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7417..7701)
                     /locus_tag="U4A12_000974"
     CDS             complement(7417..7701)
                     /locus_tag="U4A12_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1773377.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7703..9718)
                     /locus_tag="U4A12_000975"
     CDS             complement(7703..9718)
                     /locus_tag="U4A12_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1773378.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9897..10199)
                     /locus_tag="U4A12_000976"
     CDS             complement(9897..10199)
                     /locus_tag="U4A12_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN1773379.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10288..11319)
                     /locus_tag="U4A12_000977"
     CDS             complement(10288..11319)
                     /locus_tag="U4A12_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1773380.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11526..12401
                     /locus_tag="U4A12_000978"
     CDS             11526..12401
                     /locus_tag="U4A12_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1773381.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12553..13395)
                     /locus_tag="U4A12_000979"
     CDS             complement(12553..13395)
                     /locus_tag="U4A12_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1773382.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13487..15364)
                     /locus_tag="U4A12_000980"
     CDS             complement(13487..15364)
                     /locus_tag="U4A12_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1773383.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15361..16782)
                     /locus_tag="U4A12_000981"
     CDS             complement(15361..16782)
                     /locus_tag="U4A12_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1773384.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16785..20174)
                     /locus_tag="U4A12_000982"
                     /pseudo
     CDS             complement(16785..20174)
                     /locus_tag="U4A12_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            20431..21822
                     /locus_tag="U4A12_000983"
     CDS             20431..21822
                     /locus_tag="U4A12_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773385.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESRGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21895..22542)
                     /locus_tag="U4A12_000984"
     CDS             complement(21895..22542)
                     /locus_tag="U4A12_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1773386.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22550..23236)
                     /locus_tag="U4A12_000985"
     CDS             complement(22550..23236)
                     /locus_tag="U4A12_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773387.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23242..24315)
                     /locus_tag="U4A12_000986"
     CDS             complement(23242..24315)
                     /locus_tag="U4A12_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773388.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24537..25211
                     /locus_tag="U4A12_000987"
     CDS             24537..25211
                     /locus_tag="U4A12_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773389.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25216..26556
                     /locus_tag="U4A12_000988"
     CDS             25216..26556
                     /locus_tag="U4A12_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1773390.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26705..27691)
                     /locus_tag="U4A12_000989"
     CDS             complement(26705..27691)
                     /locus_tag="U4A12_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN1773391.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8141 a         5255 c         5057 g         9434 t
ORIGIN      
        1 attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag
       61 gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat
      121 taaatttcat atgttcatat gtacggatac attttcggac cgattaattt taaccattct
      181 gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt
      241 aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt gttcaatact
      301 tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat
      361 tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct
      421 ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc
      481 aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta
      541 cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc taattcatct
      601 tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta
      661 tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg
      721 cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt
      781 gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc
      841 aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt
      901 tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga
      961 ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca
     1021 tagccacctg accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc
     1081 aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt
     1141 ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact
     1201 actttacgac ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt
     1261 catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat
     1321 tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta
     1381 cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat
     1441 tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt
     1501 tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata
     1561 cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc
     1621 ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag
     1681 aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg
     1741 attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat
     1801 ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg
     1861 ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg
     1921 gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa
     1981 acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga
     2041 tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc
     2101 tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta
     2161 ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat
     2221 cctttctcca ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa
     2281 atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc
     2341 ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt
     2401 taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg
     2461 caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc
     2521 tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa
     2581 agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact
     2641 tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa
     2701 gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac
     2761 gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt
     2821 attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc
     2881 taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg
     2941 tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg
     3001 tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg
     3061 agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt
     3121 aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta
     3181 cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac
     3241 tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat
     3301 gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa
     3361 attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt
     3421 aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa
     3481 agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat
     3541 tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt
     3601 ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga
     3661 gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata
     3721 tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa
     3781 tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa
     3841 tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat
     3901 ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc
     3961 tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt
     4021 tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata
     4081 ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga
     4141 aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa
     4201 aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga
     4261 agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg
     4321 gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg
     4381 cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc
     4441 agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg
     4501 ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg
     4561 aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt
     4621 ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc
     4681 tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt
     4741 caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc
     4801 tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa
     4861 taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat
     4921 cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga
     4981 ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga
     5041 gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg
     5101 gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta
     5161 tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta
     5221 tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt
     5281 ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac
     5341 gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc
     5401 gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa
     5461 attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat
     5521 tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga
     5581 ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt
     5641 atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta
     5701 tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac
     5761 actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa
     5821 gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc
     5881 tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa
     5941 aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt
     6001 tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca
     6061 aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct
     6121 atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg
     6181 gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc
     6241 caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc
     6301 gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg
     6361 ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta
     6421 acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat
     6481 gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct
     6541 gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct
     6601 tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca
     6661 ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca
     6721 gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct
     6781 ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc
     6841 cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc
     6901 gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca
     6961 gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga
     7021 cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc
     7081 cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact
     7141 gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa
     7201 ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat
     7261 gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc
     7321 agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac
     7381 aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt
     7441 tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc
     7501 aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc
     7561 gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag
     7621 atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg
     7681 acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt
     7741 cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta
     7801 aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac
     7861 taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca
     7921 tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa
     7981 tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt
     8041 ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt
     8101 ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt
     8161 gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt
     8221 cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg
     8281 cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat
     8341 ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa
     8401 gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc
     8461 ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt
     8521 ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat
     8581 ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa
     8641 tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg
     8701 tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat
     8761 aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata
     8821 attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg
     8881 aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg
     8941 ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag
     9001 tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta
     9061 ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt
     9121 catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat
     9181 ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc
     9241 ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg
     9301 cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca
     9361 cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa
     9421 gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa
     9481 gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt
     9541 tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt
     9601 cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg
     9661 ctttttctag actgatttct cttggtgttt tcaggagata actaattttg tagttcacag
     9721 cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg
     9781 gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata
     9841 cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa
     9901 tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc
     9961 gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca
    10021 gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct
    10081 aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct
    10141 gttttagggg ccattgaacc tcttaatcgt ttgtgttttt caccgtcata atttgtcatg
    10201 ttggttcctc ctagtagttc tatacttttt catcgtacaa taagacagtt tatgcgtcaa
    10261 aaaacaaacc cgattcttac aaatatgtca tatttttcct tctgattctt gaatgctttt
    10321 gattccttct tccaaaattt tcatttgtaa gattgtttct tcatctttga tgactttttc
    10381 tgcgccatta actaatgtct cgtaaatccc ttcgtagaca cggctgtaat caccaacctc
    10441 ggaaattact ttttcttcat gataactccc ttgttcatct atataggtca atacaccata
    10501 atgttcgggc aaatcgatac caaagtcttc atgatcaggc atgtaaaaca ttttcagatg
    10561 ttcttcttgt cgatcttttg tttgtttcac gaacatccct ttttttccgt aaacgacgaa
    10621 acttggacgt tctttgatgc ggaaataact ggattttatc gaaattttca tatttccata
    10681 gaaaaaatcc agatcgaagt aatcattcat tcgtcctttc cctaaaagtt gacgtacatc
    10741 atagctgata tcatctggtt cgccgaaata agaaagaact tgatctactg tatgacaagc
    10801 atgtccatat aaatagctgt tcgcagtaga aaattcatgc gtattttctg gtacttccgg
    10861 tctgaaataa tcataatgca tttctacttc taaaatatcc cctaattttc cgctttcgat
    10921 tactttttgg actgtcaaga agtcagaatc gaaccgtctg ttttggtaac attggataaa
    10981 tagacctttt tcttttgcta atgcaaataa ctcttctgct tcctttgacg tttctgcaaa
    11041 aggtttctct actagtacat tcttaccatg ttcgagtgcc atttttccat attcataatg
    11101 aaattgacta ggtgtagtga tgacaactaa ttggatctca ggatcatccc aaagatcaga
    11161 cagctgatca gtatattcga tgtcttcgaa tgttttccac actggttcag atggggaaaa
    11221 aatttttttg atattgattt tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt
    11281 tgtactcttt ccattgccaa gataagcgat tgtaagcatg agtggtgcct ccttttattg
    11341 atgttttcag tatatcctag aaaagcgcag aaagtattta taatagctat tctggtaaaa
    11401 aagttcagaa aaacacttgt tatcattcgg tttggtgaaa ttaatgttca aagtaaggat
    11461 aaaaatcaaa aagatagtag cggaaccaag tgtttatcag taagatagaa tttggaggtc
    11521 agatcatgct tattgaagaa aaactaacaa aacaagagtt gtttaccacg acagagaaac
    11581 ggattgtcga ttacatccgt aggaatatcg aagctgcagt ctacatgacg attgaagaat
    11641 tagcaaaagc tacgtatact tcccattcag ctatcattcg tttatgcaaa aaaatgggat
    11701 tcagcggatt taaagaattc cgtttcgagc tggggcggga agttcatcaa ttgattgctc
    11761 agttcaatca aacgattgat cctaattttc ctttttcaag tgaagatagc actcagcaaa
    11821 ttgcaaagaa aatggcggat ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg
    11881 aacatgaaga tttagatcaa atcggtaaag tattgaacaa ggcacagcga atctttcttt
    11941 ttgcaaaagg tgattctcaa atcacggcaa ggaaattcca gaataaaatg gtcaagttga
    12001 ataagttttt gatcatggct gaagaataca gtgattcttc ttggaatgca gccaatttga
    12061 ctagcgagga ttgcgccatt tttattagct acagcggtcg catccatcat tatgaacgaa
    12121 taatgaccta tttgaagcat gtagaggccc ctaccttgct gattaccgga aatcagcatt
    12181 cagaaatggc caaacaggct gatatgtgtc ttgttatatc gcaggaggaa tatgattttg
    12241 ctaaagtagc gactttctcc tctcaaattg ccttcgatta cgtattaaat acgttattct
    12301 cagtgatata ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac aaacaagaat
    12361 tgatgcaaaa aggccttctt aaagaatatc ggaaaactta agaaaagatc ctcatttagt
    12421 aataagaaag atagtgtttg ataggctctc tttgatatca tcataaaaac aggctgaagt
    12481 tttctttttc aaacctcagc ctgtttgttt acggaacgat tcgaatagat atattataga
    12541 aatgaggaat agctaacttc tgtttcttat tttgaacgat tgacgaaaaa gatatataag
    12601 aaggaggaaa atcagtatac atgcagctaa caatccatac aggaaaaatc gtttgttctc
    12661 ggatactttc tgtacttctt tttgaacttc ttttgcatga taaggaaccc ggtgccctct
    12721 gacaagtaag cgatgactgt tgaccatata aggtgtacaa gttaggagtg tcacgaaatc
    12781 ctgccctttt tcgattctca gatcttttgt atcagtcggt tctaccgtct tgatctgatc
    12841 gacttgataa gcgagtgtcc gtccattgat ttcgatatag aaagaatcac ctaaagtcag
    12901 tttcggtaag tctgtgaaca actttgcttg aggcagtcct cgatggctgg aaataacggc
    12961 atgtgtattt tctccccccg taggataaga cgttccttcc aataatgagg cacctttttc
    13021 tagcagcaca gtattcgttt catcaaaaat tggcaaatgg acattgatct cagggatcgt
    13081 caaaataccg atcgtatgtt tctcaaaata ggttctatcc tttttcgcag gttctttttt
    13141 cttagtaaag ggatctgcac ctggatttcc accttctttt gctaattgct ggtttttctt
    13201 tgtcatgttt tcttggattt tagccatgac ttcttcattt tctttgaccg cttgttgctg
    13261 ataatgagag atgatctgct gatccagata attattcagc gcgtcgctaa caaatggata
    13321 agctagtatc cctattcctg agagaagcaa gaaaagcatc agcagatcta gaagtttcct
    13381 tttctttggt ttcatttgtg ttctccttgt gatgactgtt tgataataaa agaaaaagga
    13441 gcatttgcgt aatccgctgc tcctttttcc aattcttgca aaaagactaa atctgactgt
    13501 gcttgcgtct agcaaagtac agtccagcaa tcagtagaag gactactcct gcaccgatat
    13561 acacatagat tcccttaccg cctgttgaag gaagtgtacc tttgtgttta tttggtattt
    13621 tttcaggaga aatcaactgt cctgctgtta cataagattg ttcatcgata gtaaatgcta
    13681 cacggtttgt taatggaaca tatttttctg gcgctttcgt ttcttccaga tagtacgtgc
    13741 catattttag acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa accgttgctg
    13801 attctttcgc cgatacccag ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg
    13861 ctgtgtcact atcttgatca cgaacgacga attctgctcc agcaagtgtt tggtctgatg
    13921 taacgtcacc atctacttta acaaatcgtt tgcccccagt aacgacatcg actgacggtg
    13981 gtgtttgatc atttgtatgc ccgttatcga catttgcttg gttagaaaat cctttggttg
    14041 gatctgcttt ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg ccaggagtta
    14101 atgaaggaat ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc tcagtgacag
    14161 aataatttac tgggtcgatt tctttatttc catcatataa ggcataagca tacgttcctg
    14221 aagaatcatt atcaaatgtt aaagcagcgt catgagtatc gataagtttg aatgttgtgt
    14281 acttgttttg cgttccttct ttatcagcga tacctaatgg gatattgaca gaaatctcat
    14341 attgaatttt ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa
    14401 ctttagacgt atcatttttg atggtctttt ctactggtgt ttggctattt gccaggatct
    14461 caaaaggcgt gattgtttgc ttttcaatca tttccgcatt atcaggagct tttacttctt
    14521 ctaaattata ttttccaact tctaaatgat taacgatcaa ctggcctttt tcaatagatg
    14581 cctcggcaaa gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt
    14641 tggtttgatc agttgtccaa gtgtacaatc catctgtgac actttggatg tattttttga
    14701 cgcttggtgt tccttcttct ttagaaataa taaattctgc tccatttagt gcttcgtttt
    14761 cggcagtacc gatttttgta actttcaacg ttccatcatt accgactgta tttttagggt
    14821 agagatggat agtatctagt tcttctgtcc cgtatttata agagccatct gcttgtttga
    14881 tcatctcata tacagggaaa gctaaaacca tgtttgcggc agctgacact ccgtcttttg
    14941 gttcttcttt gatcgtgtag actgcatctt tcccattttg ttttttaggt aaagataaag
    15001 tgacatttcc atcagcatct gtcgttcctg aagcaactgg tgtaccagga gtcaaagact
    15061 ggactgcttg ttttgctgca tccacggacg ctcctttatc tcgttgcgca taaaattctt
    15121 gagtgacgtt ataaactgaa aatgaaatat cggctaatcc ttggtattga tcgaattcgc
    15181 tcatttcttt cccgctgttt tggattaaag gatcgggtaa atcagtcatt ttctttttat
    15241 gaaggatgac ttgagctgct gtctcctctg caaacacttt ttttgcgccg aagctgtttg
    15301 tgagtaatgg taaaataagg aaaaggactc ctaacataac gtttattttt ttatgatttt
    15361 tcattttttc actcctgttc tttttttgat aaacaaccag attgcaaagc ttgcgaatac
    15421 cataccaaca acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa gaaatggacg
    15481 gctattttct tttttattga ctactttcaa tatactgtcc gcctctttat cctcagaaag
    15541 aaaagttgaa atggtaaacg gaatttctgt ttcacttaaa atataaccag aaggtgcttc
    15601 gatctctttt aatcgataac ttcctgtttt caacccggaa attgaaaaag cgccattttt
    15661 atcggaaatc aggtgaagcg catctgattc gttctttgtc cacttgtatc cgttggctgt
    15721 ttgctggagg taatttcctg ctttgttttt aataaggaat actgcgccag ctaatcgttt
    15781 atctggttta tttgtctcga ttttgacaaa ttgcttactc cctgtatata cagagacttt
    15841 ctttgtactg acaccaaaac caaaatccag tgttccgttg ttgttgattt ccttgttcgc
    15901 ctgtgctgta ctgcttagct gcatctgata agaaatggtc agtttttttc cagcatattt
    15961 ttccaacgaa gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt gtatgacaaa
    16021 gccttctgtc agcttttctc cattcgatga aatcgttaaa ctattaggta aaaacgttaa
    16081 tgcttcatcc gcactatctg acaattcgaa cttttgatag tcaaggatat ttacaggaat
    16141 tgtcgtatgc aaggaataag tgattttttc accaatcgta aaatcatttc taggctcttc
    16201 taataccttc tcaaaaggtg gatcagggta gtcgttttct tcatttttag gatacaaatg
    16261 gatagttgaa agtttttgat tgttttgtcc atgtacagga agaacaacta ccatattctc
    16321 tgccttttct ttgacgactt ctggtacttt ggattcaatg aaaagatacg ctttatctct
    16381 tttttcttga tctttagcgg ccagagaaaa agaagcgata ccatcttcgt tgttaagagt
    16441 tgtagttgtt gcttctgcgg taaacatacc ggaagacgca ccgttttttg cgatctctgc
    16501 ttgtgcttct tctaccgttt tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc
    16561 ataaacttgg aatgtgacac catttaatcc tcgatacgtt tgtaataaag ctttttcttg
    16621 tccgtcattt ggatgatttt tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt
    16681 aatctgaacc atttctgttt ctgtttctgt tgctacctga gtaaaagctg gtttaaatag
    16741 taacaagaag agacacatac taagccaacc aagttttttc atatttagct gccccctctt
    16801 tcttttttca aaaaaagata ccaaatagaa aacgcacaac caatcatccc tactagatag
    16861 attcctaaac ggccaattcc tcccgtttca ggtaatggta cttttgcctg attcgtgatg
    16921 tctaaagaaa tctggttgtt tttggctcct ggtgacagaa cagattcatg atcttgtcca
    16981 tccacttgaa ttgacccatc ttcgtgtata actatagtaa ctggctcttt tagaccttgg
    17041 tatccttctg gtgtaaaagt ttcggtcagc gtataagttc caggttttaa attttcgaat
    17101 agaaaggtat ctgtttcttg tccatctttt ggcgattcta agtccatttc tggtccctgc
    17161 agtctgaatt ttgctccttt taatgtctgt ccgttgtctt cttttttgtg taccgttaga
    17221 tcaaacgctt ttagtcgatt ttgatgagtg agcgtccata cattatttgt catctcggta
    17281 ttttttcccg tgtaaatgat ttttccatat ttatcgattt ggaattcata atttcccggc
    17341 gaagtatcat atccggcagg accagctgtt tcaacgactc gatatttacc aggtgtgagt
    17401 gaatcaaaac tggcaagacc tttttcattc gttgtttggc tgtcaattgg ctgataagta
    17461 ccatttgctt cttttttctg tagggaaaat gctgctcctt taagatttat ctctttgtcc
    17521 gtcccttgca tcgcatactt acgtactgct acaggaactt caacaaaagg attttcaata
    17581 gtcaactgga tcgtatcatc ggaagtagtg actgtttttc catcaatggt taccgtacca
    17641 tcagaagcga tcttgatttc ccaagtagtc tttttgctta atccatgccc ttctggagct
    17701 tttgtttctg tcagcgtata ggtcatttct ttttgcaatt tgacattttc tggaagagaa
    17761 taggttccgt cgccatgatc tgttagtaaa gtatcaatag aatctcctgt taatttgaaa
    17821 acagcgccaa taagatcctt ttcacctgta gaggaatttt tcgttatttt caagtttaac
    17881 ggtgtgaatt gtttagtatt tttccatgtc attgcatcta tttgttgaga atcgtatcct
    17941 ggtacaggta attctttgat tgtttggtaa ctgaatgctt gaccttgatt attgtattga
    18001 ggtagtgata atatctcttg ataactttct ccgctatttg cagataattt gtcaatgtct
    18061 gcacgttccc acgtatttgt tgtatcttta gctggtttaa tgattcgaat atatccgttt
    18121 ttccaagctg cagcatttgt cgtatgttcc cgttgaatct caaaagtaac ttgatctgga
    18181 cgatcagcta gattgttgtc aaattcttcc cataactttt tgatgtgaag actgactccg
    18241 ggagctttag cagatggtat accgaattca gctaattcat tggtatcaat gtttggctgg
    18301 aaagttgtcc tgccgttcat ttgataccag aaatttggat ggaagtcctc attttctgtt
    18361 tggattctca cttggtaatg gatttggatt tcttggtctt ttcctaaata gatctgattg
    18421 ctcttgatgg tatttccttc tatggaaata gatggagtga ccgttgtagg acttgtcccc
    18481 acactcttga ctgaaagtgt accaggctga taaatgaatg gttctgcgat tggatcattt
    18541 atttgtccat tgcttacagt tgctgagatc tgtacagctt ttttggctag gtattcagag
    18601 atatctgttg catgatcagc tgattcatag taaagatcgc ctttttcatc tgatgaaacc
    18661 atttgtcgca tacgagactc tacttctgct tttgagagac cagctgccgg atcgctttga
    18721 agttggatgc caagaccatg tatttcgatt cctcgttctt tgagtgccat cgcttctccg
    18781 atggttgcga taaacgtact gtcgattctt ctggatagat tgttttggtc aggtgcatca
    18841 tagattcttg agattagaga agtatttccc ggccgatctt gcgtattaga aaactgagtt
    18901 ccgtaataat tgctgcttga ttgtgcgtgt acccgctgta ctttatagga aaatgttggt
    18961 acaccatccg tcagcaaaac gatcactttt ttatgtccat ttggaacgga tagcatgctt
    19021 cctgcatctc ttagtgcttt ttgtgtaaaa gtaccaccat ttgtccgtga aggtgtaatg
    19081 gattttactt gatttttcac tgaatcaaat gaacccatct gtactgcacc gttactgtag
    19141 ctatatcctt cgcttgagta gccgacatat cccatattga ttttgtctgt gataccgcta
    19201 tctgctaaag tatcgacaaa acgatcgaca ccaatcttta cttcaccgat ccgattattg
    19261 tcgttcatac ttcctgacca gtctacgacc aatacgagat caagaggcgt gatatctttt
    19321 tgtacatttc cacgagcatt caaataaaca tcaaacaatc cgggtgtact tgtttccttt
    19381 gcatactttc gtatcgcata gtctgcttga ttaccggttc ctccatattc gatatacgaa
    19441 tgggtccgat cattaggatc tccattccaa gaatttatac catcccatta atttgttccg
    19501 tttttattgc cttgatgatc aagaacattt gtatttcctg taggttgcca actggcttta
    19561 ggataagtgc cctgatcatc tgttgtatat tctgtaggaa tcgtttgatt agatggcaca
    19621 ctcacagctg gcaaggataa tgtaggcaat gtaaatgggc tgacagaatt ttcgttttct
    19681 tttgatgatt cttcttctat ttcgtctggc tttggtactg acacatctgt atctttctta
    19741 ttttctgtct catcaataat gtcatattct ttattatcga cgatcagctt gtttgcctgg
    19801 ctgtggtaaa gtgctggatc gattggaaaa ctcagatcga tagtttgttt agtgtttgat
    19861 gagatttcca acgtaatttt cccgctattt acctgataag catagccatc tccttctttt
    19921 ctttccacat cacttaagta attttcgact agaccaattt ctttttctat cttttcagca
    19981 ctggtgttat tcgcatttag cttgatgttc agtgcttttt ttccagactg atccgaaatg
    20041 acttcagctg aaattgtttc taaaactgtt ggatcatctg tagtgtctgc tattgcagat
    20101 aatggcacaa agtttgataa actcaatatt atcagcatga aaataacttt cagtttcttc
    20161 cctgtggtcg tcatttttcc cctccttttt ctttttttct gttttgacgt gaatagtgaa
    20221 aattattaaa ataaacaggc gttacataat tttatttatt ttaatatgat aattattata
    20281 cgaagggcgc taaagggaat cacaccaatt ttcataccaa ttggtttcat ttatgaagtt
    20341 gattccttcg attaatgagc gtttcattga aagaaaaggg ttgagggaga aaaacatatg
    20401 atcaggttgt tggaaggaaa ctatagaaag atggaaaaaa tcattcgaat tctgtccatt
    20461 tctgacaagt ggatgactaa aaaagaactt gccaaacgaa ttggtagttc agaatctaca
    20521 tttgtaaggt atatcgaaga gatcaaaatc cgctggggaa cggatgtaac gattcaatct
    20581 tcacaaaaat tggggtatcg tcttgaacat ttgaatgtac cagcttattt aaatgttctg
    20641 atggaaatgt cgcaaagttc tacaaacagt cgtttattaa atgagattat tgtcaatccg
    20701 ggtaaaacga ttgagtatta ttgtgaaaaa atttccatca gccggtcttc ttttacacgt
    20761 aagttgaaac aatgcaatca aattttagaa agctatttat taaaagtgat cgttgaccaa
    20821 ggttttcagt taactagcct taaaagtgag ctccagttgc gtatctttgt tacctttttc
    20881 tttttaagtt actacggaag acgcacgttg ccatttccaa tcgaaaaaaa gaagatcaaa
    20941 gaaattatgg tcaagaataa ttgtcagatg aatttgtttc aggaagaaag cccatatgag
    21001 caatctttct ttattatgta ttttgttgtg catctcatga gagaatcccg aggaactcca
    21061 tttgaatgga aagctggaga ctcttatgta cagttgataa atatcgataa aaaagacttc
    21121 ttaatcttga aaaatctctt aggaaaattg aagtacagca gctatataaa agtagtgaac
    21181 tattttgtca atacgatttt ttcagaattt tcggagaaag aaaaaaatga aatcagtgag
    21241 cagatacgaa aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa agaaaaaaac
    21301 gaagaatatg ttgttcaatt attaacgcaa atttattttt tctcccgtat tttcccatac
    21361 gatacgactc atttgacgat ccgtgcaact tactttgcac agcagatgca acaccgccag
    21421 atacgtctat atgctggatt tcgtgcagaa cttcaatttt attccactct actgaatcag
    21481 aatctgattt attatttatc tagctatatc ttttggatgc ttatcggtaa tcctgaaatt
    21541 caccattata cttctgataa aaaaatactg attatcagcg acttaagtct tcgacatagt
    21601 caatatattg aagaatatat cagtgatatt cttgctgtgc ataaaattca ctctgagaca
    21661 actgctatta ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga tctgatcgtt
    21721 acgaatcaac ccattttgaa tgcccatgtt cctcatatat tgattgatga tagtgtgtca
    21781 ttcgcaaatg aagaagaact catccgctta ttcgagttat gaataatcaa aaaacagcta
    21841 tgtataaacc accctgataa aaatttcagg acaaatacac atagctgttt ctttttattg
    21901 tattccttca gcaatccaac gagatttata gcgaaagtct tttaacaact cttcttttgt
    21961 ccaattattt gcaatgagat ccgtcaaagt gttcattacg agggtcatgg aaaagtatag
    22021 gaaactaact gcttcttccg acgattgaat ggagaaatga ttgattctta ataattccag
    22081 ccatttttcg ttcattggag aatcagccga tagttctcta cgtttggttg tctgctgatc
    22141 atttcgctgg gttaacaagt ataacctttg ccaatagata ctttcgtgat ctaagtcgca
    22201 gcaccactct aaatattttt cgatccccaa aaagaaatgt tgtttgtttt cgctaatgaa
    22261 ttttaagata tcttgatgga ctatgtgaga actgcttttg atcgtataca aataagcatc
    22321 ttccaaatct tgaaaatatt tataaaaagc accgcgcgac atccccatct tgtcaacgat
    22381 ttctgagact ttgacttggc tgatgtgctg tgagcaaaat atctccagta ataggtcatc
    22441 gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca tccacatctc
    22501 tcctttatct ctgattatac gtggatagcc cttcattgtc atgaaggatt cattccttct
    22561 ttttctcttc ttctgtcaag tacccatctt ccattcgata aacgcgatca ctccattgaa
    22621 tcatacggac atcatgagta accataatag tcgctttttg tttctcatga gcttctttaa
    22681 ctagtaattt gacgacttca tatgcatgat ctgtatccaa actagctgtt ggttcgtctg
    22741 ctaagatcaa gcttggctca ttatataaag cgcgagcaat tgccactctc tgtctttctc
    22801 caccagaaag gtctttagga taactatttt taagtgactg gatatctaaa gattgcagca
    22861 aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact agttcaaatt
    22921 gttcctttac cgttaaaaaa ggaatcaaat tagagctttg gagaataaat cctatttctt
    22981 taaagcgtaa atctgctcgt tttttttctg gaagctcagt tagtgagtga ccgttgatca
    23041 ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa gtacttttac
    23101 ctgaaccaga aggaccaatg atgctgacga attctccttc ctttacataa aaatcaattg
    23161 ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt tttggtaact tttttcatcg
    23221 ttaatatttt tttcattttt attaacctcc tattgctgta attgggtcta ctttggttat
    23281 cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc cactatatag
    23341 caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca gactagtcaa
    23401 ataagcaaac aaaagagcaa gcagaacgcc taccacccca acaataaagg actgtgctac
    23461 taaagattta gcgatgaaga aatttttgat cccttgtgct ttcattactc caaaaatagc
    23521 agtcttctga agtgtcatca cgtacatgaa aataccgacc acggcagcaa ccactaagaa
    23581 caaaaagtat atcatcgcat ttaaggtcat attttgggca gaatagccag gaatactttc
    23641 aatgaatgtc ccaatagtta ataactgtag acccttcgat gctttttctt tcgaaatttt
    23701 agctgaatcc tttattacga tcccattgat cggctgttct tcttcagaag caaacggctg
    23761 ttgtccatac ttcaaagcag tccacgtatc tagggacgca tagatgacgg gagaaactgt
    23821 gtaataagtt tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac tgccaatttt
    23881 gatcgtatct ccaattttat agcctgaatc agccagattt tgagagatga tgatttcatt
    23941 ctttgtttta aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt ctttcgttgt
    24001 gccgaaaact gtaacatttt ctttgttttt tcctttgatt gctccgctgt acaatccgat
    24061 tggtgctttc tctcctcctt caatgtattt cagatctcct cttgttaatt gtgaagctgc
    24121 aaatacttga tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat catcgattgc
    24181 ttttttaaac tcttctgaaa gtccattcgc tagtcctgac aacatgaata cgacatacgc
    24241 aatcaagaac ataataccga cgatcaagcc atatcgtaat ttagaatagc gaatttcttt
    24301 gatagctaga aacatttgtt tttcctccct atgatagatt gatagtgaca aaatgtcact
    24361 tgttgaatag tgtaactcta aagtgacaat ttgtcacttg tgttgttttt ttcttatcaa
    24421 taaattgtct aagacatctt tcgataaaac cttaacatga gtcataaaaa atttatttaa
    24481 gccttattta tgctttccat gataaactaa agtgaccaaa tcaatgaggt gggaaaatga
    24541 ttaaactatt agtagtagaa gacgataaag tattatccaa taatataaaa gaaatactcg
    24601 ctgcaatcgg cgagataaca caagtttatg atggtagtga aggactgttc gagttggaaa
    24661 gtggagttta tgatcttgca atcttggatt tgatgatgcc ggaaatgaat ggatatgaag
    24721 tgttagcaaa aatacggaaa gaaaaaatac agactccagt attgattttg actgcaaaag
    24781 atagtttgga agataagatt gaaggatttg aacgaggtgc agatgattat ctaacaaaac
    24841 ctttctatcg agaagaactg atcatgcgcg tgaaagcatt attgaaacgc tcgctaggtc
    24901 tctttgatga aaatgttctg aagtataagg atatcagttg tgatttaaaa agcaatgaag
    24961 tcttgtataa agaacaagtt ttgcccatac aaggaaaaga gtttgaattg ctcgtttatt
    25021 ttttacaaaa taagggaatc attttgacaa aagagcagat atttgaccgg atctggggat
    25081 ttgattctga tacgacgatt acagttattg aagtttatat gagcaactta cgaaaacatc
    25141 tgcgaagtac tggattggat aaagaaatca aaaccttacg taatgtcggt tatatactgc
    25201 aaagggtgtg atgcaatgaa tggagatatt acacgaaagc aacgtagtcg cttttttgcg
    25261 acaaatgtag cagcatttgc ttttatcttt tttcttttgg gattcattac tttacagata
    25321 ttaaaccaat ctgcatacag agaaacagat ctgtcattag agaaaataaa ttttgattct
    25381 cgaacagtac aattagagat acaaagatat cagcaaggcg atcccttttt aaacccgact
    25441 caaacggaac aaaacagaac aaacatggaa cagccattaa tggaaccatc aaatgaccgt
    25501 tttaacactc aagtgatctt gtggtctaaa aatggtgaga tattgaataa agcagctatt
    25561 ggtgggcgtt tcaaccaaat caaagaatta aaaatggata cgaaaaatct gaataccatc
    25621 caaaatattg aattaaatga agacagtggc tcatctgccc tctctttccg ttcgattacg
    25681 aaaaaagcgg agacgaatca agatggcgtt gcctacatcc aaattttaga aaatacaaat
    25741 caaatcaaga attcattagc gacttttcgc acgattatga ttttgtgtat gattgttttt
    25801 tggattctct ctattggtat cagttattac ttgtctagtc taagcatgcg ccctattatt
    25861 ctttcttgga gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt gcgcacaccg
    25921 ttaacgatta ttcaaaatag tttggaacat ctttttacca agcctaatca tactattatt
    25981 gaagaatccg aaagcatcgc tcaagcattg tcagagacga ggcgcctcac tggattgact
    26041 tcggatttgc tgacaatcgc tcggaatgat tctgatcagc agctgctttc aaagcaaatg
    26101 atcaatacac aagaatatat cgaacaactt gttaaaccat ttcaagagat ggccatcata
    26161 gatggaaaag agtttattct ccataacgat gcaacattag aagtaccaat tgatgaaaaa
    26221 aaaatccatc aagttttggt tattttgctg gataatgcat taaaatatac gaatccagaa
    26281 gataagatca ttttggattc atctgttaac cataaagaat ggctgattca cgtaaagaat
    26341 actggccctt ctatcagtga agaagatcaa gcaaggattt ttgatcgatt ttatcgggaa
    26401 gatcaatctc gggcgaaaga gactggcgga tacgggcttg gcttggcaat tgcaaaacag
    26461 gtaatcgaac agcatcatgg acagatttct gtatccaatt atcttccaca aggagtcgat
    26521 tttaaagtcc aattgccgtt gaagcctaaa aaataatgct tatccctaaa aaagggctgt
    26581 gacaaaaatc ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca gaagtagctt
    26641 cttcggaaat aagccgaaat ttctttaagc tgaccacttc tgtcacaacc tctttagctt
    26701 acgcttacga ttttttattg cggtcttcaa tgtgtacatc accgcttgtg tcaatatcca
    26761 attcttctcg gctaacatct tctgtcactt tttcaacatc ttcatgttct tctttatgaa
    26821 tcccaacttc ttcacgaata actgtatgtt tgcttacatc tacttgctct tctttgattg
    26881 ggatagtaat cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat tgatctgtta
    26941 ctggtttgcg ttcgatgacg atttcttcac gtttgaccgg tacttctacc gtttcagtat
    27001 cgttgaccac atgtttgtgg atgtcgactt ctccagttgt cacatcatgt gtatttacat
    27061 ctaatttttc ttcttttagt tgaattgtat catcatctag atttggtttg cgatcgatgt
    27121 cttcagtctt gcttgtaccc attgtatcag tctcgccagg tacgtccatc ggatcagttc
    27181 ctggtgtcat attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca cctacgtcca
    27241 ttggatctgt tccagtgcca gtttctaatc cagtccccat tgtgcttgct gggctaacag
    27301 tatcttgtgt atccattgta tcttctgttg tttctggctg acgatagcct tttactgtga
    27361 tgacaaattt tccatcagaa atatctcctc tatattgata taaaggatca ttttctggtg
    27421 tagccgtctc agaatcatag gtatcaaaag aaaaagtatc ttttattttt tcccataaag
    27481 aacggtcttc ttcaccatcc atccgatttt catctgtttc aacatctata ccagacaatc
    27541 cgtcaaatcc tagtctagat gctgcttcat cagtagtata aataacgata tcatctcgtt
    27601 catacccttc gttacgcaac cgttcaatta ctgataatgc ttcttccctc gtactatagc
    27661 ttccgatgac cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa tagatttatg
    27721 attggtatat tgttacttat aaatttattc tataggagac gattgttttc tacaaatatt
    27781 tagattggaa tttgattaaa acaatttaat tgaaatttga aaacatttat gttaatgaaa
    27841 gtcatattct aataaaatgt catttgtttc tatatgaatt tttatga
//
LOCUS       DASJOX010000024        26308 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJOX010000024 DASJOX010000000
VERSION     DASJOX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.024"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(151..1443)
                     /locus_tag="U4A12_000990"
     CDS             complement(151..1443)
                     /locus_tag="U4A12_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1773392.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1440..2063)
                     /locus_tag="U4A12_000991"
     CDS             complement(1440..2063)
                     /locus_tag="U4A12_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773393.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2066..2902)
                     /locus_tag="U4A12_000992"
     CDS             complement(2066..2902)
                     /locus_tag="U4A12_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773394.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2919..3806)
                     /locus_tag="U4A12_000993"
     CDS             complement(2919..3806)
                     /locus_tag="U4A12_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773395.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3814..5049)
                     /locus_tag="U4A12_000994"
                     /pseudo
     CDS             complement(3814..5049)
                     /locus_tag="U4A12_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5732..6415)
                     /locus_tag="U4A12_000995"
     CDS             complement(5732..6415)
                     /locus_tag="U4A12_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1773396.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6433..7050)
                     /locus_tag="U4A12_000996"
     CDS             complement(6433..7050)
                     /locus_tag="U4A12_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1773397.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7060..7884)
                     /locus_tag="U4A12_000997"
     CDS             complement(7060..7884)
                     /locus_tag="U4A12_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN1773398.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7877..8437)
                     /locus_tag="U4A12_000998"
     CDS             complement(7877..8437)
                     /locus_tag="U4A12_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773399.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8611..9567
                     /locus_tag="U4A12_000999"
     CDS             8611..9567
                     /locus_tag="U4A12_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1773400.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9872..10828)
                     /locus_tag="U4A12_001000"
     CDS             complement(9872..10828)
                     /locus_tag="U4A12_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN1773401.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            10932..11534
                     /locus_tag="U4A12_001001"
     CDS             10932..11534
                     /locus_tag="U4A12_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1773402.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11622..13121)
                     /locus_tag="U4A12_001002"
     CDS             complement(11622..13121)
                     /locus_tag="U4A12_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN1773403.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13137..13661)
                     /locus_tag="U4A12_001003"
     CDS             complement(13137..13661)
                     /locus_tag="U4A12_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN1773404.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13959..14708
                     /locus_tag="U4A12_001004"
     CDS             13959..14708
                     /locus_tag="U4A12_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1773405.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14699..15484
                     /locus_tag="U4A12_001005"
     CDS             14699..15484
                     /locus_tag="U4A12_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN1773406.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15453..16169
                     /locus_tag="U4A12_001006"
     CDS             15453..16169
                     /locus_tag="U4A12_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1773407.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16271..17305)
                     /locus_tag="U4A12_001007"
     CDS             complement(16271..17305)
                     /locus_tag="U4A12_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN1773408.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17309..18367)
                     /locus_tag="U4A12_001008"
     CDS             complement(17309..18367)
                     /locus_tag="U4A12_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN1773409.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(18587..19735)
                     /gene="nagA"
                     /locus_tag="U4A12_001009"
     CDS             complement(18587..19735)
                     /gene="nagA"
                     /locus_tag="U4A12_001009"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN1773410.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19811..20647)
                     /locus_tag="U4A12_001010"
     CDS             complement(19811..20647)
                     /locus_tag="U4A12_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1773411.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(20831..22048)
                     /locus_tag="U4A12_001011"
     CDS             complement(20831..22048)
                     /locus_tag="U4A12_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1773412.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(22197..22376)
                     /locus_tag="U4A12_001012"
     CDS             complement(22197..22376)
                     /locus_tag="U4A12_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773413.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            22431..22514
                     /locus_tag="U4A12_001013"
     tRNA            22431..22514
                     /locus_tag="U4A12_001013"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22465..22467,aa:Leu,seq:gag)
     gene            complement(22754..24124)
                     /locus_tag="U4A12_001014"
     CDS             complement(22754..24124)
                     /locus_tag="U4A12_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773414.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(24838..25128)
                     /locus_tag="U4A12_001015"
     CDS             complement(24838..25128)
                     /locus_tag="U4A12_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773415.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(25336..26028)
                     /locus_tag="U4A12_001016"
     CDS             complement(25336..26028)
                     /locus_tag="U4A12_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1773416.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         7941 a         5436 c         4440 g         8491 t
ORIGIN      
        1 ctatctaaaa aagcagccca cataaaaaga aggcatgaca aaaagcatgt catagcccct
       61 ttttccgaat aaatagtgtt caaaagacag cctatcggca ataaggcaag attttataaa
      121 ctacgggaag taatttccgt aaatctattc ttattctttt aagctgacaa gctcgtcaat
      181 cactactgct tttcctgaag caatagaatg atttgctgcg attcctgtca aaatcgacaa
      241 aataccatct acgtgagttg ctgcccgatt ccacggatct tcttcttttt cttcaccaaa
      301 tagatcatct aataaacgcg gatctcctcc gccatgtccg cccgcttctt gttcgacgat
      361 tacctcatat ggctcaccga acatcggata gacatggatg gactgaagaa cggctgctcc
      421 ttcatccttt ttatctccac ctgcattgat gtaagacttt tcgatcactt tcatttcgat
      481 ccgtcctttg ctgccattga aagaaacatt gaatccttcc cacggcatat acgcgtttag
      541 cgaatacgtc aaaatcgcac cagtttcata tttgaccgtt acgcctagtg tatcttcaat
      601 agagatatca tcggaaaata cactgcggtc acgaatatat ccactgtctt tttctgcttc
      661 taaatacatt tctcgcaatt cctcattggc tgacaggtct atagcaaatg gatcagcttt
      721 agctagttcg cttccatagc atcgactgta aaatatttct actccgcgtt tctccgcatt
      781 atgcatccca taaaagttta atccaccgaa tgcaaatacc ttttggggac ggctgttcaa
      841 ccaaaaattc actagatcaa aatgatgggt agatttatga acaagaagcc cgccgctatt
      901 gtgcttattt cgatgccatc ttctgaaata atctgccccg tgttgcgtat ttaacagcca
      961 ttcgaagtgg atagaaaaca catcaccaat cacatcactt tctatcagtt cctttatctt
     1021 cgtgtgatac ggtgcataac gataattaaa agtgactcgc aggcttcttc ccgtctgttt
     1081 ttggaccgtt attatttctt gagctttttc ctcatcgata gtcagaggtt tttcgcaaat
     1141 aacatcatat ccttttttca ttgcacgaat gatataatca tgatgcgttc gatctactga
     1201 cgtaataatg acttcagccg gccgtacgtc atccagtagt tgctcaaatt gtgatggcat
     1261 acatgtcttt acttttttct gtccatactt gttcacaagt atccgatttg cataatccat
     1321 ccttgtttga gatacatcac aaaaaccgac tagttcacaa gtttgatgat acgtagaggc
     1381 aattgcctca taaaaaaaac gcgctctccc accaagtccg acttgtacat attttttctt
     1441 catttaaagt cattctcctt ttcttttaag caaaatactg tgacaaatgg cagccctgga
     1501 tacagtaatg cagtgagtaa aaaatcaggc tgtatacacc agaacagtcc atacaaaagg
     1561 cataaatacc acagaaaccc cttgacgttt tttgacaaca gccagatatt ccttttgatc
     1621 aaataaacaa gactatctgc tttatcttct ttgcagatat caattagcgt actaaaaaaa
     1681 ctatgggata caaaaagcaa cagacacaat agaaaaggaa tcagatacca agctccaatc
     1741 tttcccacta ataaatacca atcaactgca aaaataatga caaacagctg aaacaacgta
     1801 atgatgaaac acatccgttt gattttccct ttcctccaac ataccttttt ttctgccaag
     1861 taatggacag atagataata agaaggatac atcatcaaaa aataaatcag atagacaaca
     1921 cgagcagaca gcactaatcc taaagtgaac aaacatccta gaactaaagc gaaaagagca
     1981 ttactgataa gactggttaa aaaaatccga cagacatagt agttgagctt gtaaagaaac
     2041 atctcttcta ttggcttttt catattcagc ccttcagacc gcttgtactg atcccctgga
     2101 caagatattt attgaagaat aagaaaatca gaaaaaccgg tagcagcgac aacgtagaca
     2161 tcgcaaacat actcgcgtaa gtcgtactag atccagcgtc tgcaaataat ttgatggcaa
     2221 tcgataccgt gtaagatctc ggcttgttta aataaatcaa tggtcccatg aagtcgtccc
     2281 atttccaata aaactggatg atgatcgtcg tgatgatcga aggtttcagc aaaggaaaaa
     2341 taattttggt aaagacacta tacttgctgc atccgtcaat aatcgctgat tcatctaact
     2401 ctttggggat gctgaccatg aattgcatga tctggtaaat gaaaaatgct tgtccaaaaa
     2461 aatgtggtaa aatcagcggc acatacgtcc caaccaaatt cagccgattg taaataataa
     2521 actgcggaat caaaatcact tgtgcaggaa tcatcatcgt tactaacatg acgacgaacc
     2581 agaatctttt tcctttgaaa tcgattctcg ataatgcgta agcaatacaa gtcgagctca
     2641 gaactgtccc tatcgtcgaa actgcagaga tgacaaacga attcctaaag aaagtaaaaa
     2701 atgaaattcc agcaaacccc gcccatcctt ttgcgtaatt ttcccattga aaagtctctg
     2761 gaatcaaatt cagcgaacca ctcataatct cttgatccgt tttaaaagaa cctgcgatca
     2821 tccataagac cggataaatc atgactagac ctaatagcaa aataaaaccg tattgaaacc
     2881 atttcgttac tgtattattc atttcttcct cctctcattc acacaacgac ccattttttt
     2941 gatgatttga aaatcagaaa ggtgacagct cccataatca acagcatgat ccacgaaata
     3001 gcactagcat accccataga attaaaagaa aaagcttggt tatacacata aagagcataa
     3061 aagttcgtgg catcattcgg accaccttgg gtaatgataa atgcctgcgt aaaagtcata
     3121 aacgccgcga ttgtctgcat aaccaagtta tacaaaatga tcggcgaaag aagaggtaac
     3181 gtgatcttgt aaaacgtctg ccatttgttc gctccatcga tctctgccgc ttcataatac
     3241 gagcctggga tctctttgat acctgcagca aagatgatca ttgccgaacc gaattgccaa
     3301 gcagacatca aaacaagagg gatcatgacc agtttttgat cgctaaacca ggaaagcgca
     3361 ggtgcgccta atcgttccag gaaaatattc actaacccgt ttttagaaaa caactcccgc
     3421 caaactagag cgatagaaat actgccaccg atcaaactag gcacataata caaagaacga
     3481 aagaaagtcg ttcctttgat attcctcgtt aaaagataag caatcaatag accaaagatc
     3541 agtttcacag gtacagatgc aaatacatag agaaaagtga ctcggatgga gttcaaaaaa
     3601 cgggcatcac tcagcatctt tatataatta tccaagccga tccatgagac tggatttccc
     3661 aaacgataat cggtgaacga ataataaagt gacaagatca tcggtatcac tgttatcaat
     3721 agaaatccta ggatgaatgg agaagcaaat acgtaaccga ccacattgtc ttgatccaag
     3781 aaccgtttta gtttttcttt tttcatgcct tccttctgct gttacttctt cataaatgac
     3841 tttttcaact aaaagattgt attggtcttg aatttccgga ttcaaagcac tatcaaagac
     3901 attcacttcg ccgttgtcca tatcggaaat catttctaaa taatcaaatg tcgcttgcaa
     3961 agttgcatca tctgcacgag cgagctgttc tcgaatattc gacatgattg gtacaccacg
     4021 ttctcctttt aaaatttcat tggcttcttc actgttttga aagaaatcaa taaacttagc
     4081 agcttcttct ttatgggctg agctttttgc catactcagc atttgcgaag aacgtaaagg
     4141 gataccagat tctccatctg cttttctgcg aggtggatta atcaactgaa tctccatatc
     4201 ttcaggagcc cctgacaata tttctgtcaa ctggttactt gatagaaaca ccattgccgc
     4261 ttctctatcc ctaaccgcct gaaaagacag atccgtcgta cttgcagctc caccacctgg
     4321 ttctggatgg gcccctttgt ctaccagatt tttacgcagc tggaaaaaat ctgttagata
     4381 gtctggattt tcaaatccca agctgttaga tagatcttcc cgattataaa aattcaatcc
     4441 tttctcaact tgtggtattt gaataataca gccagctata aaatcttcga atcttgaaat
     4501 agcatagatt cctaattctt cagtgatttg atagctgatt tgttcaaatt catcccatgt
     4561 ccaatttgaa gctggttcag ggatatttaa ttgttcaaat atatttttat tgtaagcaat
     4621 cgcaaacgaa ttcaccccgt tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc
     4681 taaataggat tcatctgcat tagaaatatc aattatccct tgctccacat attcatctaa
     4741 tggttcaatc tgattgatgt attgaggaaa ttgattgttc agttggaaaa tgtcccagat
     4801 atcatctgat gctgctagtg tacctaattt tgtgtaatat ccttcataat cataaaattc
     4861 ataatcgatc gtcacatggg gatttttttc ttcataaagc tcgattactt ttactgttgc
     4921 atcgtgtcgg acttgcgaac cccaccaagc catgttcagc gtgatttttt tgtcatcttc
     4981 ttgcccacaa gccgtcaaga agaataacat aacacttaaa gctaagatga ttctcattct
     5041 tttcttcatt ctctgcacct ctacttccat tgttattttt caatatactg tatttttata
     5101 cagaacatat ttattaaacg atctttttca aggggaataa aaaccaagac ttgccttatc
     5161 gttctgccta ggatgaacaa ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc
     5221 tttcatcgct aaaaacactt cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa
     5281 ggatatcaga caactgccca atatccttga catagctaaa taagcatgct tcttcgtgtg
     5341 cgctcttccc taaaaaacag aaaaataact agtaattctg tatgaaatta ggtacaatat
     5401 acacgtcgat gcacaagcat tgactatggc aacttgattg gtcagttccc gctgactttt
     5461 caagccccga caatttagct gctactaaat tgtcgggtgc cttttttagt ttttattcgt
     5521 ttttcattct atagaaagcg cttcaataaa tcaagacatt tttttgttag aatgctaata
     5581 ataacaaaag gttttaactc ttcttatgta cattacaggt aaaaaaacaa aaaaatttct
     5641 cttttttttg aattctgaac aaatagtact ggagtacaaa agacgaggaa gagccactat
     5701 ctgctcttcc tcgtcttttg attctcacct gttattcatg attctttttc tcagtttccc
     5761 gatatttttc cattttacga ctaaaaagct ctccgctact tggtggcgta tatgtgatgg
     5821 catttgctcc tgcttgaatg gtctcagcaa tactttcttc cgttggacca cctgtcgcaa
     5881 tgataggaag ttcaggatat tttttacgaa aatggcgtac tgcttctgca gtttctttac
     5941 ctgcactgat attgataatc gaaataccag ctgctaactt ttcatctatt tctgtatatt
     6001 tcgacgttac cgtactgata actggaatat caactactcg acaaacttct tttactgtct
     6061 ctaaaggcgt aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc
     6121 tcatgtagga agaacgagca ccttgtgtca aaccaccacc tacaccggag aaaactggaa
     6181 tgtcagctgc ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa ggatacacag
     6241 caattactgc attcgcattt gtattacgta tgatcgcaat atccgtagta aagatgatcg
     6301 aacggatcaa ttttccaaat atcttgatcc cgcttgcttc taaaatgatt tccggtactc
     6361 ttacgatatc ttttctcaat tccgtcatga tttctggcgt aaattgttct gccatcagcc
     6421 acttctcctt tgctatctcc ggtcatactg attcaattga tacgtctcga tgacctggat
     6481 gattttttgc gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc
     6541 ttcctgatag ttggtggccg taatcacttt ttcatacagc gcaggattcc aatctactcc
     6601 ttggacgaac agttgtgtat ggtcatccat ggactgttcc catgaatcat acactttgaa
     6661 gtctccttgg atagttatcc attcttcgtt aacaaattcc tttgtttcca ggcttacttt
     6721 cttctgatcc ccataagcct tgatcccgaa aagattattg tatttgctag ccaaggtact
     6781 ttttccccaa tttgattcta atatagcttg tcctaaaatg atactaggaa gcactccata
     6841 accatcttgt agttcttggg catgcggaac aagccgttca ataaattgct gatgggattg
     6901 cgcttcattt tcttgctgat tatcagtaaa aggagaagac agattcttca aggaaaaaac
     6961 aaaggctaag agtatcactg ctacaccggc aaccactgct ggccaacgga tattcttgcg
     7021 cttttgcttt ttccactttt ttttcgccat gatcaattcc tacttttcat ttaattcatt
     7081 cagatattct tctagcgtca attggtgttt ggtgatatac tcagcgtttt ctttaccgac
     7141 ataacgtaag tgccaaggtt catatgtgat cttagttata tcttcgtgtc cttttggata
     7201 acgaacgata aatccatact gcgcagcatt atctgcgatc catttagctc caggctgatc
     7261 tccgtaactt gcatccaata cagtagaagg ataactgttg taccagtttt gatcgacgac
     7321 atcgactgcc agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg tttctttagt
     7381 aatctttgtc gcctcctctt ctgatacacc ttgacgactc atcacatctt ggacattttg
     7441 ttcgaaaact tgttgttggg atgatacaga ccggtaagct gagaccatca ctaatggaaa
     7501 tccagctgct tcagcagcct tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat
     7561 caagtatccg ttacttaatg atgccaattg agtagactca tcaatttctt tttctaattt
     7621 gtgatctgga ccaaccaaaa ctagcttcca atcgtctgtt ttaacatctg gcaattgttt
     7681 ggacgtacga tcttttgttt ctttgtcctt taccttgctg ctggttgttt gactgttctt
     7741 ttctttacta gcagttgttt tggtgcttgt tcgttcgact gcttgattgc cggcttgaac
     7801 atgcgaatca tcatcgctaa aaataacata gcccattgct gcgatcataa tcacaactgc
     7861 tccaaatcct aaaaatttat tcacttgaaa aatcctttcg ttgttcttca cgttccccat
     7921 tcgcctcatg aacgtaagtt tcttccagat tttcattcac ccaatcgaga agttcgattt
     7981 tttctccttc gatctgtttt tgaacatctg ctggcagacg aaaattcaaa atatcatcat
     8041 agccccattc atcatattga atacttactt tcaagtcaag gtatctacca gcctgctgat
     8101 tatttttcaa agggatcagc tcgacttttg ctgctttgct gctaagccat ttttgcgcga
     8161 tgcgtgtttt gatttgcttg tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa
     8221 tcgtttgacg caaaatcttt tgcgttagca tccattcata atcagtgaaa agatttttta
     8281 ctttctgcat cgcttcagat ggcagatttt tctcgccatc tttccatttt tgccattgtt
     8341 ctggactgat cagcaagtat tcatctaaaa aatttttttc agtttcaaac tgttctaata
     8401 atacactcat aataagatca atatatactt gatgcacgtt tatcccctcc ttttaactat
     8461 tctacttaag ttttgatcaa aaagcacctc ttaaatcgct gctttcattt tttttaatgt
     8521 ttcaagtcct ctcctcttta ttcttcgtga tttttgttat aataatagac agacaagaaa
     8581 ctcaatccaa ctatgaagga gcgttttcag atgaaaaaat atcaatgggg aatcgttggt
     8641 cttggtacca tcgcacatga gtttgccgaa agcttcaatc aagagacgag cgaactaaca
     8701 gcagttgctt ccagaacttc agaaaaagcg gaaaacttcg ctcaccgtta caatattcca
     8761 aaagcttacg gaagctatca agaaatgtta gacgatgctg aaatcgacat cgtgtacatt
     8821 gctgtaccaa atagacagca tatcgaccat atcttggcag cattggaagc aggcaaacac
     8881 gttctatgtg aaaaagccat cacgatgaat aaaaaagaac tggctgacgc tatgcgctta
     8941 gcagaagaaa agaatttgat cttagcagaa gcgatgacaa tcttcaacat gccactttat
     9001 cagcagttac gctcaataat ggatacagga aaactggggg ctttaaaaat gatccaagca
     9061 cctttcggca gttacaaaga accagaccct aaaaaccggt tcttcaatcc agaattagct
     9121 ggaggagctc tgttggatat cggtacgtat gctgtatctt tcgctcgttt ctttttgagc
     9181 tctcaacctg aagtagttgc ctctacaatg gttccttttg aaacaggcgt ggacgaacaa
     9241 tccgtcacta tcttgcgtaa caaagaaaat gaacttgctg ctgtcagctt gacttttcag
     9301 gcaaaaatgc caaaagtcgg tatcgttgcc tttgagaacg gttatgtgac gattgctgat
     9361 tacccgcgtg ctgatcgtgc ggagatctta tttacagatg gtacaaagga attcatcgaa
     9421 agcgggaata catctgaagc aatgaattac gaaataaaga atatggtaca catgattgaa
     9481 ggtgacttgc ccaatcgctc tctcttcttg acaaaagatg tcatcgaaat cttggatcaa
     9541 atgcaagttc tatggaaaaa tatgtaattt taaaaggttg tgaaaaagct gttctgcatc
     9601 aagtaataag aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ctggacttat
     9661 taccgcagag gaaagctttt gaacaccgtt taagaaagag gttgtgagcc taccgttcag
     9721 ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac
     9781 ttgtgaccga ggagttgaca ggagaacacc gtttaaagaa gaggttgtga aatcagcttt
     9841 ttgacctgaa tattagctga attcacaacc tctatttttg gattttttgt tgggagaatt
     9901 ctgccttgat ttcaataaaa cttttaaaag actgcaaaaa ctggaacaat tctgctcgat
     9961 cctcgaattc agggcgacta gtcggcagag gcatttgccg atacaataca taaacttctt
    10021 caatacgtgt taaaatttgt ctgccatcat ttgactctga aaatgtttct gcagtataaa
    10081 tcaataaacc atagatatgt tttccataat aagctggttg ctggattctc tctagatttt
    10141 ccagcatgtc tttaatcaca ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt
    10201 atagcggctg tctgagccag tagttttctt gatgttctct ggcatcttct tggctttcgc
    10261 gaatgaatgt cagtaaatgt tcgcatttga tctgcacatc ttctttattt tctgataaaa
    10321 gaaattctgc catttgtctc aaaatatttc gaaattcttt ttcgatctgt atttggtttt
    10381 ccttcagctg cttcgtacga tctggcatgt aaacatttgc aagcaaagca aatactaatc
    10441 caatcgccat taatcccgct tcattgccga tcagctgcca agacatgttt tcctccacta
    10501 aataatgagt gaccaaaacc gaatttacta ctatcccttc ggttaatttg aagcgagagg
    10561 caagcgggat gaacaggaat aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa
    10621 aaaaactgaa cagtgctgtt gccgtagcta atataaaggc tgcgattcga ttcatgcccg
    10681 tacgcaatgt agagcgcttg gtatttccta cacttaataa tgcaatgatc cctgcagctg
    10741 gccaatacaa cagcgataag ctacccgctg tgaccatcga aaagaagaca ctgatcactg
    10801 ttttgactgt tcgcattcct atacgcattt acttccccct tttttatttt aattatactt
    10861 gaataatgaa aaactagcta tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa
    10921 ggagaatctc tatgcaattt ggccgttttc gactaggtat gcgtacgatc aaaagtgcct
    10981 tagctgtttt tttatgtatc ttgttttttc atttgactca acgaggaatc ccgatgattg
    11041 ctgcattatc tgctgttttt tctttgcgtc aggacttgac tacaaccgtt tcttttggaa
    11101 agtctaggat catcggcaat acattaggtg gtacattagc tatcatttat ttttttgtca
    11161 aaagctattt tcacaatgat tttttggtag aattgatctt gcttcctatt ctagttatta
    11221 ttgtgatcgt cgtgtcagat ggtattaaca ataattccgg tatcatttcc gcaatagcta
    11281 cgttgttgct gattgcgttg agcatccctc aaggcgaatc cgctttgtat gctatccaac
    11341 gtgtactaga tacttttata gggacattca ttgcaatcgg tatcaatttc tttcttcgtc
    11401 ctcctgaaac agaaaagaag gaagaactat cagaagattt ggtcgaactc caaaagaaag
    11461 aacgattgct aagaacaaat ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa
    11521 atagcgagga ctaattttct tttgcaataa aataaaaaaa tggagagaag aactttaaaa
    11581 agtcttcctc catttttgat tgaacggtga agtgtactac tttattcttc cttcatcgag
    11641 ttttgttctg cctcttttgg cgcttcatac atatttactt ggctagtagt cccacgtgtc
    11701 gacaaattct ctacgatttc cttcaagtca agtcctgttg tcgcttttag tgtttcttga
    11761 gtagaagaaa gcaaatttgt ggcataattt gtcactcggt tcgctccact ggtttctcct
    11821 gaactgcttc ctgtatcgac aacagagatt ttttcgatat ttcctagcgg ctgtgctgct
    11881 tcgcgcatca attgcggtag catatcgata accatactca agacagctgc ttcgccatat
    11941 tccttgaagg cattggcgat cttttgtttt gcttcggctt ctgcttttcc ttttgctagt
    12001 gctgcttcgg cttctgcttg tccagctaaa cgtgtcttat tggcttccgc ttctgccagt
    12061 gcttccacac ggaatcgttc tgcttcggct tcagtcactt ctttgacttt ttgagcttgt
    12121 gcttcttgtt ctttagcgta ccgatcggca tctgcttttt tcttcacttc cgaatcatat
    12181 tgcttttcac gacgtgtgat ttctttttct tccagttcga tttgtttttg gcgttctacg
    12241 attttaatct gcatttcctg ttcgattact tgttgttgtg ctcgggcact ttctaaattg
    12301 taggcttggt ctgcttttgc tttagcaaca tcttgttctt gtttgtaggt tgctaatttc
    12361 agttcttttt cttttaatga ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt
    12421 gattcttttt ctgcttgagc ttttttgata cgcgtttctt ttagagcttc cgcttctgct
    12481 atatctgcat ctcttttgac ttgggcgatt cttggtttcc ctaaagagtc aagataaccg
    12541 tttttgtctc tgacctcttt gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg
    12601 tctacagaag cgacttcttg tacgctttga ctgaacttat cgcgattttg atagatttct
    12661 tctacagtca tagaaccaag aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca
    12721 ttttccagtt cttctcttgt cttgcctaaa aactgttctg ctgctgtagc aatctcttct
    12781 acagatgaac cgattttgat gatcgaagta ccatcacaca tcaccggtac gccttgttcg
    12841 gtatacactt caggggtgga gacgtccaat ttactcgata atagactgat ccgattcgaa
    12901 cgttgaaaaa ctggcaagac gaatgcgcca cctccacgca caattttgat tttgttattg
    12961 ctttcatcaa tgtgcacatt tttgcttcct aaataacttc cactgatgat caacgcttca
    13021 tccggttttg ctgtctggta cttcgctaca aagacgatca gcaacatcag taaaatcaaa
    13081 gctgcgattg cgatcgcgat aaaaacaact ggtatttcca tttaacacac gctcctttaa
    13141 gcttttatca tgctttcata aggcatgaca taacagattc tttccttgat ctcaatgatc
    13201 aatactttcg ttccccgctc agctgtctgt tcttgggggc tgtaaaaaca agctggacga
    13261 ctaatggaac cggtaacact tttcaactga atttctccca tacctcgtac agggatcggt
    13321 gtgatcacaa cagctacttg tccttccaga gttttttcag aagacgacat cgttgattcg
    13381 gcatgtttca tcggcatcaa tatatagaaa ttaagcaaaa ataccaacac tatcgatatg
    13441 cctccactag caataaataa aaataaagat gattgatctg ttaaacgctc acctagaaaa
    13501 ccaaataagc ttgtgaaagt taaccaaggg ataataagca tcggatcaag cgggccatca
    13561 aaatcgaata catctccaaa aataatcaat aggactgcca atcctgcaca aatcagcaat
    13621 gtataccaat aaatcgtctc aagtgcgatt ccccctatca tatacacgcc tccttccatt
    13681 tctacgtcta tcataccaat gtttgaaaat tctgtatagc ttcttttacc gactcagtgg
    13741 tgttttttat aggaaggttg tgtttatcgt tgacaatttg ttaaattttt gttttcatgt
    13801 taaaattttc acaataaatt ctttcgaggt agcctatgtt tccttttttt aagaaaaaac
    13861 cattaaagct ccagaacacg atcatccttg tcgtttgttt ctccacattc gtttctttga
    13921 ttgtttctgc catattgatc aggaatttta ttctttcgat gaaccgatct agtgtgtcag
    13981 ataaaatctc tagcattgct aaaatcgttg ccaatgataa actcatccaa aaaaagctgc
    14041 agactggtga cccgacacat gcgattcaag gaataacaaa tgaaatcatg attgcatcga
    14101 atttagattt tatcgttgtc atggaccctg aaggcattcg atattctcac gcagacccaa
    14161 gttacatcgg tcttcctttc tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag
    14221 aatttttctc tgaaaaagaa ggaatgttag ggttcggctt ccgctatttc gtccctattt
    14281 attatgatca agagattatt ggtgcagttt gtgttggctt aacaaacaac acgattgaca
    14341 atcaagtcaa agatggacaa aaaatgattg ttttttcatt gattattgga cttagcgtag
    14401 gcatcatagg cgccgttttt cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac
    14461 cacaagaaat agcagaacag ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag
    14521 gaatcttagc catctcttct gataaaacta tcctcttgat caatacacct gcaaaaaaca
    14581 ttttctacca aacagagcag ctattggatt tcgaagtagg aaaaaaatca gtgataatct
    14641 atatgacgtt ttgtttaaag agacgattga gcatcaaacg accgttcgga ctcaatccat
    14701 gtatttaaat gggatagaaa ctatttctag cgttacacct ctttttagca atgaccgttt
    14761 tttaggagca gtcgcaactt tacgaaatcg atcagaagta atgaaattaa tggaggagct
    14821 tggggggacg aaacaatata taaactccat gagagctcaa acccatgaat tcatgaataa
    14881 acttcatgtc atttctggtt tgattgattt gaagaagtat gaagaagtca cccattttat
    14941 ccagcagcta aatcaaaatt ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt
    15001 acctgcaatt gccggttttt tattaggcaa aataaacgaa gccaaagaac aatctgttga
    15061 aatgctgtta gaaagcgatt cttttatccc taaaatggag atggatgact ccatccattc
    15121 acttttgcaa gtgttaggga acttacttga taatgcaaaa gaagccgtgc tacaaaaaca
    15181 tgaaagaaac ggacaggtcc ggattcattt aaactatgac acagaagctg aagtattcat
    15241 cgtgaccgtc tgcgatggcg gtgtcgggat atcagaagaa atctcagaaa aaatatttac
    15301 gctaggtttt tcgacgaagg gaatcaatcg tgggtttgga ttgaatctca tccattcaat
    15361 cgtcaaacgc cacggtggat ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt
    15421 ctatctagaa ttaccaaaaa aggaagatca aaatgatgac aaatgtcctg ataatcgaag
    15481 atgatccaat ggttgcagaa ttgaatcgta cttttgtgga aagcatccct aatatgaacg
    15541 taatcgaaag tttcagaaat gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag
    15601 atctgctgct gcttgatgta tacatgcctg gcgaaagtgg tatccatttt ttatcttggc
    15661 ttagacagca aggaaatgat atcccagtga ttttgatcac tgctgcagat gatatcgaaa
    15721 ccataaagaa tgtgatccgc tacggtgcct tagattattt gatcaaaccg ttcactttgc
    15781 aacgttttca gctggcgatc gaaaagtatc ttgctttctc tcagacaatc caaacgactg
    15841 aaaaagccga tcagaaattg atcgatcaag tattcaacca caccactccc attcacgaaa
    15901 taccatcatt catcgatttg cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg
    15961 catttattct aaaagaagat actatttttt caacaaaaga actggctcaa aaaagctcac
    16021 tgtcacgtat atccgtcaag aaatatttga attttttagt agaacaagat atattgaacg
    16081 aaagaatcaa atatttggaa atcggccagc cgctaacctg ttatgaattg acaaagaatg
    16141 ggcggaaaaa gctcacagat tactgttgag tcatcgaatt atcccataca aaatagtcca
    16201 agtatacaga agtaacaact gtttacttgg actattttta aacattttat tgacgaatat
    16261 ccatgttttc ttatcttaat tgacgggctt taggaagaga tacctcattt ttcgtttttc
    16321 cagtttctat tatttcacga aagttctcga aacttgtcga aatcatattt gataatgcct
    16381 catctgtatt tgaacctaca tgcggggtta caagaactct agggtacatt tctactaatt
    16441 gctggatagc agggtctgga atcgtctccc atggcttgaa ggatcggaaa aaaacctctg
    16501 tttcatttgc aaaaacatcg gttgcaaaac cagctaaatg attgcttttc aaagctctta
    16561 aaatagcttc attatcttga agctctccac gggctgtatt aatcaagatg ctaccttttt
    16621 tcatttgagc taaaaatcct tcattgatca tttgatcgtt ttcacctggg atatgaggta
    16681 catgaatact gacaatatcg cttttggcta ataactcttc aagcgagtgg aacgtcaata
    16741 cttttttagc agcttctgtc tgatgaatat cgtagccgat cactttggct cctagtccct
    16801 taaacaaaac agcttccgtc aaccctatcc tgcctgttcc tatgatccct actgtgcaat
    16861 ttctgatttc acgactaaac atttgctcat ccactcgaaa atctttgaaa gatgttcgga
    16921 tcgtcgtata cgcagtattt ctcaaaagca tcatggcaaa agtcaaagat aactctgcta
    16981 tcgcatttgg cgaatacgat ggtacacggg ctactgtcat accaaaatca gctgccgctt
    17041 gtagatcgat atgattgact cccacagtcc gtgtaaaaac ataacgaatc ccatattcct
    17101 gcatttttgc aagattggtc tcatctgcta tacaatttcc cctcaacaat acagcatcat
    17161 ggtcctttgc tgacacgata ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat
    17221 aatggtaaat attcaatttt tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg
    17281 cgattctcat actttcttcc tccatttctt atgaaaataa acctatgcca tgaatcattt
    17341 gtaatacaac gatccataca ggtgtcagta ccacagcaaa aatagttgac agtaaggatg
    17401 cattggaggc taaaagtgcc tccctatcaa aactgatagc ataggctgct gccactgttg
    17461 cagctggtgt agccatcatg ataacgattg ttgccagtgc ttcaaaattg actggtagaa
    17521 tattcgttgc cgtcaaaact gctagaatca cgatattcaa tgcaggtaca aataccactt
    17581 tgactagact gtaataccat gaagtttttt cagatgcagc ttttttgaag cttacttcac
    17641 ctaatgttgc gccgattgct agccacgcca atggtgaact taaaccagct aaataatcca
    17701 tcggctgaaa tagccacggt gcagtctggt caattcgcaa aaacgctact tggcttatct
    17761 ctccttctgc acctgtaact gacacttgtg gcagataccc ttggaacagc caaatgaaaa
    17821 ggccggcaaa agtagcgata atgattggat tgacgaacat cgctttgata tttttagctt
    17881 ccatcttcaa gccactcatt tttatgtatc cataagaata caaaaagatt cggtagccaa
    17941 tattgaaaat tgaggaatac atcacgccga caggaccata aatcgcactg acgataggcg
    18001 ttccgaaaaa ggtcgtagaa ccaaagatcg tcaggacacg caacgttgtt tgcttatccc
    18061 ctttatagct taagaaaaaa ggttttgaaa ctagaataag aataatataa ataactaatc
    18121 cccaaatcaa gacattgatt ccttgcatca gtaattctgt attgatatct tgcatgaaag
    18181 ctcgaaatgc cagtgctggc aaagccactg ttaagacgac tttcgataga atttttccaa
    18241 cagattcaga aaaaatcccc ttttttctac aaacaaaccc taataatata ataaaaactg
    18301 ttgaagtgat tgcactgaca atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata
    18361 agttcatctt tctcctccta attatgttca tttattcaca ataaaaaaag cttttatttt
    18421 gctaatacgt attaacattt ttaattttat ctttacattg ttacttattg aacaatagga
    18481 aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaacctt
    18541 aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac
    18601 gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt
    18661 ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg
    18721 gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat
    18781 ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg
    18841 ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc
    18901 caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta
    18961 ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat
    19021 gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac
    19081 cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta
    19141 gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa
    19201 actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag
    19261 cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg
    19321 cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc
    19381 ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc
    19441 ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca
    19501 tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat
    19561 gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc
    19621 cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg
    19681 catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat
    19741 tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg
    19801 tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga
    19861 atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat
    19921 cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt
    19981 ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt
    20041 gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac
    20101 tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac
    20161 taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta
    20221 atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa
    20281 caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat
    20341 cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca
    20401 acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat
    20461 caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct
    20521 cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt
    20581 gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg
    20641 tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag
    20701 gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa
    20761 aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg
    20821 gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt
    20881 gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat
    20941 taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt
    21001 tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag
    21061 cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc
    21121 tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta
    21181 gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca
    21241 tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc
    21301 ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca
    21361 aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat
    21421 agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta
    21481 ataattcttt tggataaagt gccccagtag gattattagg attaatgata acaatagctt
    21541 ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt
    21601 catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg
    21661 gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca
    21721 tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa
    21781 aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat
    21841 ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg
    21901 cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt
    21961 cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta
    22021 atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag
    22081 aactttccaa attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt
    22141 gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa
    22201 ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt
    22261 cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc
    22321 attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt
    22381 gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt
    22441 tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag
    22501 tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga
    22561 acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc
    22621 atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc
    22681 aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac taaagcgagg
    22741 gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata ccaagcacgg
    22801 ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt agctctttaa
    22861 ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc cgcattccgg
    22921 aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc attatcatca
    22981 aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt ccaacaatta
    23041 aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga taaaaaagtg
    23101 acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc caaatcattg
    23161 cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca aaataaataa
    23221 tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt tgacatttcc
    23281 tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc atgtctaaaa
    23341 aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt
    23401 tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc cctggagttg
    23461 tcaaagagaa aattaattct gctaagctta ttattgaaaa tactgataac ttataattat
    23521 atcgtccatt gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt
    23581 tgacttgttc ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc
    23641 tatttatttc ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg
    23701 ctactggcca ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca
    23761 atggtattaa acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa
    23821 aaacatactt gcaaagtaat actacaattc ctaacataat cgtagaattt aataatttcc
    23881 atagcataaa gtgtttagaa aagaacatta agaatagttc tataataatt cttgaactcc
    23941 atgtcttata atgttctatc gaagcaccca aatagccaag ttcatctaat ctacttgcat
    24001 aaactacgtc atccgctctc atagtcggca tatacatatt aaagagtaaa aagatacccc
    24061 aaatccctat tagtaacaag atcagttctt tgttattcgt tatttttcta ttatgtttgt
    24121 tcatttttat actccttctt atatttccta atagagtata acatagcttt aagcattact
    24181 taaagataac ttaaaagtta tcttttaaat acttaattgt taaatacctt atttgatgca
    24241 aaagaaatct acgatagtca acaaaaaaat tttcattata tccagaaaaa tttttcttag
    24301 ttaaggacct atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca
    24361 atcatattgc ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa
    24421 tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt
    24481 acttttacga ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt
    24541 taaaatccta caattccact acctgagact agtaatgact agtctctttt tatggcctct
    24601 gatcccccca ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca
    24661 atatccctca aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta
    24721 gttatatata gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc
    24781 tgcatccatc tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta
    24841 gtttattctt tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac
    24901 cacaattttg ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt
    24961 ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact
    25021 atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact
    25081 aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc
    25141 aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg
    25201 actttttgtc tattaccttt cttaaaatca tattggcact tcctgcgaag atgaaggaca
    25261 tgtactaaaa aaaagaggca gcagtataga ttactgttgc ttctttacta atttaggttt
    25321 acttgttaag aaagattatt tacgcaaatg ttcttcaaca atttgctgag atttatcagc
    25381 tgtatcttcc acttcatctt ttgctttttc taacttttga atatattgtt tcaagcttga
    25441 gttttcagca gttgcttcgt ctaatttctt ttgtgtatct gttaactgag tttctacatt
    25501 tgttttttct tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa
    25561 ttctgaaagt tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt
    25621 gatttcatca tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct
    25681 ttgtagttgt gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga
    25741 ttgatttttt tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt
    25801 tgaatcttgc aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taagcgcttg
    25861 ctctttatct gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc
    25921 tgtctctcca ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag
    25981 acatagaaat gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc
    26041 ttaatccaat acttttacat gctaaaagta agtgatctca aacacaatgt caagcgtttt
    26101 taaattacat gtgtaatttt aatataaata ctatcaaata ctcatttaat cctccttttt
    26161 tatctattta aaaaatcttt tttatagaga aaaatgcagg ttaatacttt cttaaataaa
    26221 tatttatggt gaacaaaaac aaaaaagcaa gttttattaa acgtaaccct tttcatttca
    26281 tagattactg tatttattac aaaaaacg
//
LOCUS       DASJOX010000025        26132 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJOX010000025 DASJOX010000000
VERSION     DASJOX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.025"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..310
                     /locus_tag="U4A12_001017"
     CDS             <1..310
                     /locus_tag="U4A12_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1773417.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
     gene            783..1337
                     /locus_tag="U4A12_001018"
     CDS             783..1337
                     /locus_tag="U4A12_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1773418.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1510..1983)
                     /locus_tag="U4A12_001019"
     CDS             complement(1510..1983)
                     /locus_tag="U4A12_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1773419.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1995..2531
                     /locus_tag="U4A12_001020"
     CDS             1995..2531
                     /locus_tag="U4A12_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1773420.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2571..2942
                     /locus_tag="U4A12_001021"
     CDS             2571..2942
                     /locus_tag="U4A12_001021"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1773421.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            3226..4497
                     /locus_tag="U4A12_001022"
     CDS             3226..4497
                     /locus_tag="U4A12_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1773422.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4742..5767
                     /locus_tag="U4A12_001023"
     CDS             4742..5767
                     /locus_tag="U4A12_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773423.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6061..7170
                     /locus_tag="U4A12_001024"
     CDS             6061..7170
                     /locus_tag="U4A12_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1773424.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            7253..8149
                     /locus_tag="U4A12_001025"
                     /pseudo
     CDS             7253..8149
                     /locus_tag="U4A12_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            8165..8989
                     /locus_tag="U4A12_001026"
     CDS             8165..8989
                     /locus_tag="U4A12_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773425.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9021..10331
                     /locus_tag="U4A12_001027"
     CDS             9021..10331
                     /locus_tag="U4A12_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN1773426.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10479..11738
                     /locus_tag="U4A12_001028"
     CDS             10479..11738
                     /locus_tag="U4A12_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1773427.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11748..12620
                     /locus_tag="U4A12_001029"
     CDS             11748..12620
                     /locus_tag="U4A12_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773428.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12632..13462
                     /locus_tag="U4A12_001030"
     CDS             12632..13462
                     /locus_tag="U4A12_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773429.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13502..15685
                     /locus_tag="U4A12_001031"
     CDS             13502..15685
                     /locus_tag="U4A12_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1773430.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15746..16966
                     /locus_tag="U4A12_001032"
     CDS             15746..16966
                     /locus_tag="U4A12_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN1773431.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16963..18423
                     /gene="gtfA"
                     /locus_tag="U4A12_001033"
     CDS             16963..18423
                     /gene="gtfA"
                     /locus_tag="U4A12_001033"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN1773432.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18500..19606
                     /locus_tag="U4A12_001034"
                     /pseudo
     CDS             18500..19606
                     /locus_tag="U4A12_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19928..20137
                     /locus_tag="U4A12_001035"
     CDS             19928..20137
                     /locus_tag="U4A12_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1773433.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20130..20279
                     /locus_tag="U4A12_001036"
     CDS             20130..20279
                     /locus_tag="U4A12_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN1773434.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20433..21098
                     /locus_tag="U4A12_001037"
     CDS             20433..21098
                     /locus_tag="U4A12_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1773435.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="U4A12_001038"
     CDS             complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="U4A12_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN1773436.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22801..23034
                     /locus_tag="U4A12_001039"
     CDS             22801..23034
                     /locus_tag="U4A12_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN1773437.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23031..23438
                     /locus_tag="U4A12_001040"
     CDS             23031..23438
                     /locus_tag="U4A12_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1773438.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24575..25093)
                     /locus_tag="U4A12_001041"
     CDS             complement(24575..25093)
                     /locus_tag="U4A12_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN1773439.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25460..25708
                     /locus_tag="U4A12_001042"
     CDS             25460..25708
                     /locus_tag="U4A12_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773440.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8921 a         3983 c         4843 g         8385 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc
      361 cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga
      421 aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt
      481 cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta
      541 agaacttcta cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata
      601 agtaaggtta atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata
      661 aaatcataaa ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa
      721 tgattatggt aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa
      781 tagtgagcta taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt
      841 ggctaagaag aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata
      901 atgggttacg tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta
      961 agcctattat tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga
     1021 gagaagaaat tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc
     1081 ggcaacaagg tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag
     1141 aaaaattagg acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact
     1201 actacaagaa aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag
     1261 caattacaaa aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat
     1321 ttaaactagg tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta
     1381 tttttatcta gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt
     1441 acttggtata ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa
     1501 aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa
     1561 gaaaaatact gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc
     1621 gtttttgacc atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat
     1681 gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat
     1741 atcatccata gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga
     1801 cgcaccagat tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg
     1861 ttttaagtta ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag
     1921 gggcttattt tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct
     1981 catttttaga tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc
     2041 gaccgacatt tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta
     2101 atcaagacga tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa
     2161 ctgaactgac tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga
     2221 ttgaacgaac gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt
     2281 ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac
     2341 gttttgaagg gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata
     2401 ttgatgatta tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa
     2461 ttgatgccat tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg
     2521 taggctggta gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct
     2581 tagaaaatca agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc
     2641 cagttgccat tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat
     2701 ctgatttaca acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa
     2761 aagatcgtga attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa
     2821 ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg
     2881 aagaaattcc tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat
     2941 aattagctaa tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa
     3001 gaaaaacgca tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa
     3061 actatcattt tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt
     3121 tatacctctt tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt
     3181 ctcaactatt tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg
     3241 caagagaaac aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt
     3301 accagcgtat ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct
     3361 ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt
     3421 cgtccagagg ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag
     3481 attggtggca gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt
     3541 gtgttgtttt catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat
     3601 gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata
     3661 ggattgcttt atttttcggc tattgataat gctgattttg tattttctga tccatttacc
     3721 gcagaagttt tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg
     3781 cataatgtta attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg
     3841 ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt
     3901 cctgttgttt atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa
     3961 atgaatcgtt tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat
     4021 ttgattcatt atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa
     4081 ccaagtgtta ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta
     4141 acctttaaat cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta
     4201 gctgaattta ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt
     4261 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
     4321 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
     4381 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
     4441 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
     4501 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
     4561 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
     4621 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
     4681 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
     4741 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
     4801 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
     4861 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
     4921 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
     4981 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
     5041 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
     5101 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
     5161 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
     5221 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     5281 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     5341 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     5401 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     5461 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     5521 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     5581 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     5641 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     5701 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     5761 cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta
     5821 gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac
     5881 gcattcaaaa caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata
     5941 gctgttttaa ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac
     6001 gggcaagtaa ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa
     6061 atgttagatg aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct
     6121 tgggatcaat attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta
     6181 gtaaccatgc actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt
     6241 gatgatgtta tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat
     6301 ttctatacgc ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta
     6361 tggtacaaca aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca
     6421 tgggaaacac tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt
     6481 tttgcatcac atattggaac agagactggc tactggccat ttatttatca aaatggggga
     6541 gaagtatttg ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct
     6601 ttagcttttt atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta
     6661 caagatcctg ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga
     6721 aactggtttg caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc
     6781 gtgttaccta agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt
     6841 gcgagtacaa aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac
     6901 gccaataaaa tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac
     6961 gagtgggctg cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat
     7021 ggagtccctc gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt
     7081 attaaccgta ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa
     7141 gaagtcaatg cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa
     7201 ggattctatt tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa
     7261 tcgaattttt ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc
     7321 aacggcatta ggattaattt tattaaatat atggccagca attcaaacag caattttaag
     7381 ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa
     7441 aatgtttgca gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt
     7501 ttcgatacct agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt
     7561 taaaggattg tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc
     7621 agtcgcaatg atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt
     7681 ggctaaaata ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc
     7741 tattatcatt gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg
     7801 cttataagaa attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat
     7861 ccagcagttt ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt
     7921 aacaacagtt atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa
     7981 tagtaccgcc atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat
     8041 tttaaatgac aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat
     8101 tattactgca attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca
     8161 aattatgaaa aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg
     8221 tattgcaatg gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga
     8281 atctacccaa attccaccta caattttccc tgagaaattt cagctaagta actatcaaca
     8341 agtacttgat ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg
     8401 acgagtgatt ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag
     8461 ttttccaggg aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc
     8521 catttttatt actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc
     8581 agcattaata ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt
     8641 cttaggttta cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac
     8701 gatctttttc aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt
     8761 cacagcgttg tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga
     8821 taagatgcct ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc
     8881 acaattaatg gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt
     8941 ccaaagaaaa ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt
     9001 agtgattgga gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt
     9061 ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg
     9121 cattgtacga tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta
     9181 ataaaaattc gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt
     9241 taagagaggc tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag
     9301 tgattgattt tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg
     9361 ggattggtgg gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag
     9421 aaatggaaga agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt
     9481 taaccatggg tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc
     9541 aagtatgcgt accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg
     9601 actttcaatg gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa
     9661 atggggaaga tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc
     9721 cagattctgt acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg
     9781 aacacaatgc agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg
     9841 aattagggat tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg
     9901 aaagcatgcg agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat
     9961 atgcttcata tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg
    10021 tcttaaataa aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt
    10081 tagtcgataa aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg
    10141 ctttaaatcg gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag
    10201 ataaatcaaa aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt
    10261 taacagatat caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg
    10321 aatataatta aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag
    10381 ttaaatttag taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa
    10441 aagaaaacgc attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta
    10501 ttttagcttg tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg
    10561 aagatgcaag cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa
    10621 cgattcagga gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg
    10681 tggatgtacc gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag
    10741 atgtcattaa tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt
    10801 tagaggactt aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt
    10861 ttgctattga ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact
    10921 ataacaaaac tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg
    10981 aaaaaatagt agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag
    11041 aaggctggac tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag
    11101 aggaagcgaa caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag
    11161 aaatgcaaaa agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca
    11221 actggcaagg tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga
    11281 ttatgccgaa tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag
    11341 taggaacttt tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag
    11401 acctagcctt atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag
    11461 aatatatgac aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg
    11521 ctgttgaggg cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg
    11581 cttttactga tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt
    11641 ataccttaac aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa
    11701 atgctttctt taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag
    11761 aaattttaaa tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga
    11821 tttttttcta ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc
    11881 tgacttataa ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga
    11941 agtttatcaa atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa
    12001 ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct
    12061 ttagagcatg gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta
    12121 aacaaatttt taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac
    12181 aaaccagtct tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt
    12241 ggcaaggaat tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag
    12301 aaattattga agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg
    12361 aaatcaccta cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct
    12421 taacagcatt tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat
    12481 ctttggggtt acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg
    12541 ctattgcttt agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat
    12601 cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc
    12661 taaatacttg cttttattat tagggggctt catgatttta gttcccttag tttcaacgct
    12721 attcggttca tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc
    12781 agcgactttg gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa
    12841 ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc
    12901 ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt
    12961 aattttagga atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag
    13021 taaaattggt ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc
    13081 acagacacta tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga
    13141 ggctgctgaa attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat
    13201 gttaaaacca atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt
    13261 catgctacca ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca
    13321 atataattat acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt
    13381 aggaattgtc acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat
    13441 gagcaatgga gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact
    13501 tatgattata tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta
    13561 tctattagga atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac
    13621 gcactattct ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc
    13681 aaatgcgcaa gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg
    13741 acatggtgat tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa
    13801 tgattttcgt tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc
    13861 agccttatat acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc
    13921 gattagtgga ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat
    13981 tcgttctacg caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag
    14041 tcaatcaatt gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc
    14101 aagagaacgt caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa
    14161 acgcggatca agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga
    14221 tgaatttcaa ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt
    14281 gattcaaaaa gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt
    14341 tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag
    14401 taatgaaggt ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat
    14461 tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta
    14521 ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat
    14581 tgaaatgttt gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt
    14641 aggtgattgg gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca
    14701 agtgcatgat aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga
    14761 tagtgaacta tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc
    14821 aattagtcga aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta
    14881 tcaacaaatg acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa
    14941 tcgaaatatg acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc
    15001 tcatcgttat atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac
    15061 tattttattt gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta
    15121 catgccacaa acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata
    15181 tggaaccagc ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa
    15241 tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt
    15301 ttttggttat gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga
    15361 acaagttgcc ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt
    15421 ggaaagtccg tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga
    15481 agccatggtc ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa
    15541 attagcagga ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga
    15601 tgaattgatg aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca
    15661 acgcttttat ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa
    15721 gagactcatt ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc
    15781 tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg
    15841 gttagataaa ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact
    15901 agagtatggc atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat
    15961 tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc
    16021 acataatagt atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat
    16081 tggtgaagat ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc
    16141 tgaaactggt gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat
    16201 ttttacgaat caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca
    16261 cgatcaacca cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa
    16321 aaaaatgttg gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca
    16381 agaactagga atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac
    16441 gcattctcag tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc
    16501 tgtcaataaa cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa
    16561 tgccagattc tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact
    16621 caatgcagaa gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat
    16681 taaattaaga aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt
    16741 gaaagataaa aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt
    16801 agttatctta aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca
    16861 agtattaatc aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg
    16921 accgtttgaa agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa
    16981 gcaatgctca tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc
    17041 ttagacaaac atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg
    17101 acaggtgatc gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca
    17161 tgggaagatg tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat
    17221 cacatttctc gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca
    17281 tacaaagaaa tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa
    17341 gatattgatt taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg
    17401 gatggtacga cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg
    17461 accaaagaag ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc
    17521 tgttctttaa ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat
    17581 ttcttcgtgg aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa
    17641 tatgacgtcg aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc
    17701 aatcatgatt attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat
    17761 agtggaaaat cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca
    17821 acattggata cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa
    17881 gaattggatt acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat
    17941 tcaagtgcca attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca
    18001 ttaggcgata atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg
    18061 attccacaaa tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa
    18121 gcaacgaaag aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa
    18181 gaagtgcaac ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct
    18241 gcggcgtttg atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg
    18301 attacacgta aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg
    18361 aaagaattta tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt
    18421 taacctattc catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac
    18481 caataaaaag gactcataca tggataagta taaaacaaat acagcattta ataaatggtt
    18541 ttctgccata aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa
    18601 ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa
    18661 tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact
    18721 agaaaccgat gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga
    18781 acctaaagtg ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa
    18841 accaaccaag cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa
    18901 acgtcataag tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta
    18961 ttgttatatg gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga
    19021 acatgatctc aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga
    19081 tcgtgggtat taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt
    19141 cactcggatt aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga
    19201 aacagaaatt ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa
    19261 tttccgacta gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg
    19321 aatggacgtc tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt
    19381 gtttttcaag cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa
    19441 aggcgtgcag aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa
    19501 attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact
    19561 gtttcagccc tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt
    19621 ggctcgttac ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat
    19681 cctctgcgtc agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt
    19741 ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt
    19801 tttgagtatt aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa
    19861 tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg
    19921 tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca
    19981 gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta
    20041 cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa
    20101 gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga
    20161 aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa
    20221 acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat
    20281 tatggaaaag aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa
    20341 ttattcctac cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct
    20401 acaatcaaaa aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg
    20461 acattcaaaa gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca
    20521 tgtatgggat ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa
    20581 atcctgcaaa agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa
    20641 aagaagaatt tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg
    20701 gttttatttg tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat
    20761 taacttggaa agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt
    20821 ataagagagc caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa
    20881 tcgaactaga cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa
    20941 taggaggcat gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc
    21001 gtcatattat gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat
    21061 taaggcatag tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag
    21121 aaaggttaaa acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa
    21181 attcattgag agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa
    21241 tcaaaccgac acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac
    21301 caaaagaaac acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt
    21361 ccgcctattt atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc
    21421 tatatgggta tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa
    21481 taccaatgaa tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg
    21541 gctgacttga attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc
    21601 ggatatacgc ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt
    21661 tactttatat cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc
    21721 tgctatattg acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc
    21781 taaagatagg tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa
    21841 aatcagtgca ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg
    21901 tttactcaaa gtatttttat attcattggc aatataatct agataatttt gtgtatcctt
    21961 atctagaaat aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt
    22021 gatggttgct acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt
    22081 ggaacttttc gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt
    22141 agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt
    22201 tacattttct tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt
    22261 taactgtctt atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc
    22321 ataaaagagc ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc
    22381 ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat
    22441 tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga
    22501 gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa
    22561 atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata
    22621 aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc
    22681 ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc
    22741 ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact
    22801 atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca
    22861 aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa
    22921 aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata
    22981 tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc
    23041 attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc
    23101 ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc
    23161 gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac
    23221 ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag
    23281 ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg
    23341 taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag
    23401 tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta
    23461 tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa
    23521 atccaactat atagaaaacg taaattttct atatagttgg attaagataa cgactattta
    23581 ttttatcata tttttactat accctatact tttacttggt atatttatga ataatcgcaa
    23641 atttacccct atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt
    23701 cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata
    23761 aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt
    23821 gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat
    23881 cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta
    23941 ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt
    24001 atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc
    24061 ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa
    24121 caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc
    24181 ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg
    24241 ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa
    24301 aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca
    24361 taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa
    24421 tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa
    24481 ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc
    24541 tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc
    24601 ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata
    24661 cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata
    24721 gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa
    24781 tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact
    24841 ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt
    24901 catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag
    24961 caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa
    25021 attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt
    25081 tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata
    25141 atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct
    25201 aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg
    25261 tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc
    25321 atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac
    25381 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
    25441 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
    25501 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
    25561 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
    25621 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
    25681 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
    25741 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
    25801 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
    25861 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
    25921 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
    25981 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
    26041 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
    26101 acttatatgt tttagtgaac tacccccact ta
//
LOCUS       DASJOX010000026        25620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJOX010000026 DASJOX010000000
VERSION     DASJOX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.026"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..494
                     /locus_tag="U4A12_001043"
     CDS             12..494
                     /locus_tag="U4A12_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN1773441.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            531..1541
                     /locus_tag="U4A12_001044"
     CDS             531..1541
                     /locus_tag="U4A12_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN1773442.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1656..2369)
                     /locus_tag="U4A12_001045"
     CDS             complement(1656..2369)
                     /locus_tag="U4A12_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773443.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2559..3149)
                     /locus_tag="U4A12_001046"
     CDS             complement(2559..3149)
                     /locus_tag="U4A12_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN1773444.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3633..3860
                     /locus_tag="U4A12_001047"
     CDS             3633..3860
                     /locus_tag="U4A12_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN1773445.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3876..5042
                     /locus_tag="U4A12_001048"
     CDS             3876..5042
                     /locus_tag="U4A12_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN1773446.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5276..6319
                     /gene="hrcA"
                     /locus_tag="U4A12_001049"
     CDS             5276..6319
                     /gene="hrcA"
                     /locus_tag="U4A12_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN1773447.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6413..6976
                     /gene="grpE"
                     /locus_tag="U4A12_001050"
     CDS             6413..6976
                     /gene="grpE"
                     /locus_tag="U4A12_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN1773448.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7027..8856
                     /gene="dnaK"
                     /locus_tag="U4A12_001051"
     CDS             7027..8856
                     /gene="dnaK"
                     /locus_tag="U4A12_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN1773449.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9007..10173
                     /gene="dnaJ"
                     /locus_tag="U4A12_001052"
     CDS             9007..10173
                     /gene="dnaJ"
                     /locus_tag="U4A12_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN1773450.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10255..10920)
                     /locus_tag="U4A12_001053"
     CDS             complement(10255..10920)
                     /locus_tag="U4A12_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773451.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10896..11069
                     /locus_tag="U4A12_001054"
     CDS             10896..11069
                     /locus_tag="U4A12_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773452.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11114..11851)
                     /locus_tag="U4A12_001055"
     CDS             complement(11114..11851)
                     /locus_tag="U4A12_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1773453.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11853..13355)
                     /locus_tag="U4A12_001056"
     CDS             complement(11853..13355)
                     /locus_tag="U4A12_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN1773454.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13614..15608
                     /gene="uvrB"
                     /locus_tag="U4A12_001057"
     CDS             13614..15608
                     /gene="uvrB"
                     /locus_tag="U4A12_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN1773455.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15618..18434
                     /gene="uvrA"
                     /locus_tag="U4A12_001058"
     CDS             15618..18434
                     /gene="uvrA"
                     /locus_tag="U4A12_001058"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN1773456.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18697..19962
                     /locus_tag="U4A12_001059"
     CDS             18697..19962
                     /locus_tag="U4A12_001059"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN1773457.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            19966..20697
                     /locus_tag="U4A12_001060"
     CDS             19966..20697
                     /locus_tag="U4A12_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN1773458.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            20832..21716
                     /gene="rapZ"
                     /locus_tag="U4A12_001061"
     CDS             20832..21716
                     /gene="rapZ"
                     /locus_tag="U4A12_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN1773459.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21713..22711
                     /locus_tag="U4A12_001062"
     CDS             21713..22711
                     /locus_tag="U4A12_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN1773460.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22745..23680
                     /gene="whiA"
                     /locus_tag="U4A12_001063"
     CDS             22745..23680
                     /gene="whiA"
                     /locus_tag="U4A12_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN1773461.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24223..24873
                     /locus_tag="U4A12_001064"
     CDS             24223..24873
                     /locus_tag="U4A12_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773462.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25060..>25620
                     /locus_tag="U4A12_001065"
     CDS             25060..>25620
                     /locus_tag="U4A12_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN1773463.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8455 a         4339 c         5479 g         7347 t
ORIGIN      
        1 gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata tattttgagt
       61 tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag aaagaacaag
      121 gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat aataactacg
      181 tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag atcgtcggta
      241 aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa aagcagggcg
      301 ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa gagctcttat
      361 caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc atccaaacgg
      421 ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg ccggcaaacg
      481 aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac atgataagtt
      541 atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca ggcgtatcag
      601 aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa tctttccaag
      661 tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac
      721 gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc tttccgactt
      781 ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct ctacaaccaa
      841 aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca ttaaaaacag
      901 ggatagccac tgcacatgga actaacctta ttgatttaag aggagagtac ttggatgaaa
      961 cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt ctacagcaat
     1021 cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc atcatcttct gttttccatc
     1081 tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg aagggacgat
     1141 tattaggtgg ttgtattgat acgatcaggc atttaatcgg tactccttat ggagatgtcc
     1201 aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta gaaaactgtg
     1261 agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca ggctggtttg
     1321 atggcagctc ggggatcatg ttcggtagaa gtgcggcaga ggatttgtta gattataaaa
     1381 tgaaggatgt atacgaagat ctgtcaaagg aattaaaact accgatcttg tatgatattg
     1441 attgtggtca tgtaccgcca caactgacat tgattaatgg tgcctatgca gaagtagcgg
     1501 tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt tcctgccttg
     1561 gagtctatca aagaccaata aatctaaata gaaaagaaag tgaaagaagc tgacaaaatg
     1621 ctgtcagctt ctttttgtat aacatattag aaagtctaat tatctataac tgtcacatcg
     1681 attaactgtt tcacgtcatt gggatttcct gtaggaatag ctactacaga tgcattatca
     1741 atgatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc tgcaggcatt
     1801 tgccaatgtg aaggaactgg ataactagtg attgtcccgt catggttact tgaatacact
     1861 aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg atagactgca
     1921 ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac atgtaattcg
     1981 aaatcacccg atgtatctct aaaagtatta cctctaatag ctagccaaac acgtgcgtac
     2041 tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga ctgtgaggag
     2101 gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga tgtagaagaa
     2161 gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt agaagctgat
     2221 ttctgagtac ttgatttttc agctgaactg gtttgtctga ttgaactttt gtctgtggtc
     2281 gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa taaagagacc
     2341 aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt ttaataccag
     2401 tatagcataa tcctgaattt ttgaatccgt aaaaccttgt ctatgaatat aaatagagtg
     2461 tatcttcttt gataaatgtc agaaaaagtg tgacaaataa tcatcagtaa aattacttgt
     2521 cacactattg ataaggagta tttaattgaa gagaatgatt aggagaaagg atctccgtac
     2581 tttttatagt aatcatagaa tccaagtttg tatagacgaa tcgttttttc tcttatttct
     2641 tctgtttcaa aatggactgc ttgaagaatc aagtctgtga attctatagg agctgttcca
     2701 ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt ctataaaatc ttctttattc
     2761 tgataatttt ccgattctaa ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc
     2821 aataatccgc tctgagctaa ataatcaact gccccacaaa tagcagctac tggtacgtct
     2881 gtttttaaca atttatcgat tatttgatat aaatgatgat tttttatgtc ccatgaattg
     2941 ccaccgatca taataaaaag gtcaactttt tcaggtattt gttcgattaa gtaatcgatt
     3001 tttgttttta atcctccaat ggaagtgacc tctgattgga tagaagcagt tttgttttgc
     3061 catttctcat tttggtttag tgtgctagta atataagcac cttcccaatc ggcatattcc
     3121 tctaatagaa acgtaatcgc agttttcatt ttcaagctct cctttactat ctataacaag
     3181 gacaacagtt ttaacctaca gacattcatt aaaattgggt agcgacagcg aaaacaacaa
     3241 ttcttaagtt ttagacagag tatacagata gtcaacttaa ttaaatacga actaaataaa
     3301 aatgacaggt aatcattttt gtttaccagc attataacat tttgcataca tggatgacaa
     3361 aagaaccaat tcccacttgc ttgagtacaa tattaaaaga aactggaagg caaaaagtaa
     3421 ttatagaaac gaaaaataaa tcatatcggg cactcagaca tgaaaacaac cctagagata
     3481 tataactagg taaccagttt aatgaaagaa aaaatcatcc aagaagtcga ttcttggatt
     3541 ttttagtgga tttgttcgcc aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa
     3601 gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat agcttttcat atttttgcag
     3661 attttgactt aatgccgaag gtcagctttg cgattaccgt ttatcgagag gctgtaaaag
     3721 caccgtgtag ctccgatgac aataagcaag catttttaga gctaggtttc cttgattcta
     3781 aatatgcggc ttatttcaat gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca
     3841 ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata aatcccgcag
     3901 aaaacaaggt ttctgcgtac attcatatcc cattttgcga gcatatctgc tattactgtg
     3961 attttaataa agttttttta gaagggcagc cagtagatga gtatgtggaa atgctgctga
     4021 atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa tttagaaaca ctttacgtag
     4081 ggggcgggac accaacttct ttatctgcta aacaacttga tcgactgtta tcaggtgcta
     4141 gagagatcct gccttttcaa aatggtaatg agtttactgt agaagcgaat ccaggtgatt
     4201 tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt caatcgttta tccatgggag
     4261 tgcagacatt tgataatcgc ttattgaaaa aaattggacg taaacacacc gcagaagatg
     4321 tgtatcaaac tatgcaattt ttagaagcag aaaattttac caatgtaagc atcgatttga
     4381 tttatgcatt accgggacaa acactagaag gatatctgga tactttggaa aaagctttgg
     4441 ctttggatct gcctcactat tctttgtact cattgatcct cgaaaacaaa acaatgttca
     4501 tgaactgggt acgacaagga agactcgaac tgcctgatca ggaaacggaa accagaatgt
     4561 ttgaagagac gattgaagca atggaaaagc atggacgtca ccaatatgaa ataagtaact
     4621 ttgggctctc cggacatgaa tctcaacata acctcatgta ctggaacaat gaccactatt
     4681 ttgggtttgg agctggtgct agcggttatc ttggagatat tcgttaccgc aacaaaggac
     4741 caatccaaca ctatttacgc ccattacgag caaatgaacg cccggtattg gaagaagaaa
     4801 aattgacacg aaaaaatcaa atagaagaag aaatgttctt aggactaaga aaaaaaacag
     4861 ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg
     4921 atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt
     4981 tgacaccaag agggaccttt ttggggaaca atgtgtttga gcgctttttg tttgaaaaat
     5041 agtttaaaga atatttgttt tttatctgaa tttaaataaa aaaagttcaa aaatacaata
     5101 aaggactatt gaaacgttga tataactgga tttacatcaa ttatttagaa ttagcactct
     5161 tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca ggagaagggc ttcttgctat
     5221 atttataagt ggattagcac ttagaatata tgagtgctaa tgagaggtga ggaagatgtt
     5281 aacaaagcga caaaacgaca ttttacgtct gatcatccaa cactatacga gtagtggcgt
     5341 gcccgttggt tccaaaacat tgatgtctga gggagtcaag gccagctcag caacgattcg
     5401 caatgatatg aaagcattag aagatgaagg attgctgttg aagactcact cttcttctgg
     5461 gcggattcca tctgctttag gctatcgtta ttacgtggat catttgttaa aacctgctcg
     5521 cgtagcagaa gatgatttgc tgctgattcg tcagtctcta ggcagagaat ttcatgaaat
     5581 caatgagatt attaagcaat ctgctgagat attatctgag ctgacaagct atacgacttt
     5641 ttcattggga cctgaaataa aagaccgccg actgactggc tttcgtatcg tacccttgaa
     5701 taatcgacag attattgcta tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt
     5761 cactcttccc gaatatatgg gaagtcagga actagaaaaa atggtacaga ttgtaaacga
     5821 ccgtctagtc ggagatcctc tgatgacggt gtaccataaa cttcgtacgg agattccgat
     5881 gattcttcat aaatatttcc aaacgacaga agggatgtct cagttatttg acaccgttct
     5941 tggtcatgtt tttgaagata aggtttatgt cagtggacaa atgaatctct tggattatga
     6001 gccccgtcaa gatcttgatc agttcaaatc gatgttttct tttatgaaag actcagacgg
     6061 acttacacaa atgatcactc cgatggatag caatattcat atcaagattg gtgctgaatt
     6121 aggcaatgat ttattgcaga atatgagtat gattcaagca agttacgaga tagatggtca
     6181 tggaagaggt acaatagcct tgttagggcc tactagtatg ccttattctc ggatgttcgg
     6241 tctagtcgat gtttatcgaa gagaactagc agctaaactt gctgattact accgttcgct
     6301 tgacttgtca aactcgtaat gtgaataacg aagctatttg cagctgatcg ttctctcttt
     6361 cttagtaaga gcaatcaaac agctgactga ataatgaaag gaagactgta tcgtgaaaga
     6421 aaatcaagaa gaactagata aagaaatgac agatgctcag ccagaaccgg aaatcgatgt
     6481 cgaagctgca gaagattcgg gtatttcaga agctgaagcg gaagaattcg aaacagcgaa
     6541 attaaaagct gagttagaag aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc
     6601 aaatatggca aatcgaggaa aaaatgagcg tgagcaatta caaaaatacc gttcacaaga
     6661 tttagccaaa aaattacttc catcaattga caacctggaa cgtgcattag ccacagaagt
     6721 gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg
     6781 taacgcactg gaagaagaag gaattgaaaa aatccctgct aaaggagaag cctttgatcc
     6841 taatcttcat caagcagtac aaactgttcc agcgactgaa gatacaccag ccgatacgat
     6901 tgtagaagta ctacaagaag gctacaaatt acatgatcgc gtgttaagac caaccatggt
     6961 cattgttgca caataattgt gcggatataa aacttacact ttcaattaaa gaaggagacg
     7021 ttaattatga gtaaaattat cggtattgac ttaggtacga ctaactctgc agtagcagta
     7081 ttggaaggaa acgaagcaaa aatcatcgct aacccagaag gaaaccgtac aacaccttct
     7141 gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg
     7201 acaaacccaa atacgatttc atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta
     7261 gatgtagaag gtaaatcata tacaccacaa gaaatctcag caatgatcct tcaatatatc
     7321 aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca
     7381 gcttacttta atgatgcaca acgtcaagca acaaaagatg ctggtaagat cgctggtcta
     7441 gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc ttgcctatgg tttagataaa
     7501 acagatcgcg acgaaaaaat cttagtattc gaccttggtg gcggtacttt tgacgtatct
     7561 atccttgaac taggagacgg tgtctttgat gtattgtcaa cagctggcga caaccatcta
     7621 ggtggggacg actttgataa caaaatcatc gactacatgg tagcagaatt caaaaaagaa
     7681 aacagcattg acttgtctca agacaaaatg gctttacaac gcttgaaaga cgctgctgaa
     7741 aaagcgaaaa aagatttatc tggcgtatca agcacacaaa tcagcttgcc atttatcaca
     7801 gcaggagaat ctggaccatt gcatttggaa atgacattga cacgtgcaaa atttgatgaa
     7861 ttaacagctg acttagtaga acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca
     7921 ggtttatcac aatcagaaat cgacgaagtt attttagtcg gtggatctac tcgtatccct
     7981 gcagttgtag aagcagtacg taaagaaaca ggaaaagaac caaacaaatc agttaaccct
     8041 gatgaagtag ttgctatggg tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa
     8101 gatgttgttt tacttgacgt tacaccattg tcattaggta tcgaaacaat gggtggagtg
     8161 ttcactaaat tgattgaccg caatacaacg attccaacaa gtaaatcaca agtattctca
     8221 actgcggcag ataatcaacc agcagtagac atccatgtat tacaaggtga acgcccaatg
     8281 gctgctgaca acaaaactct aggaagattc caattgacag atattccagc agctcctcgt
     8341 ggtattccac aaatcgaagt aacattcgat attgataaaa acgggattgt aaatgtatct
     8401 gcgaaagatc taggtacaca aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta
     8461 acagacgaag aaatcgaacg tatggtaaaa gatgcagaag caaatgcaga agcagataaa
     8521 gctcgtaaag aggaagttga cctacgtaat gatgtagatg cattgctatt ctctgtggac
     8581 aaaacattga aagaattaga aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca
     8641 gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa
     8701 cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta aactatacga acaagcggca
     8761 caacaacaag ctcaagaaaa cccagaagca gctcaaggcg gagcagatga tgtcgttgat
     8821 gctgattttg aagaagtcga tggcgacgat aaataatcag ttcactgaat ttcgagagaa
     8881 ggccaaagtt gctactttgg ctttttcttt tgaaaaatag tttgaagtat cggtttttgt
     8941 ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat gatgagaacc aataggagga
     9001 aagcctatgg caacaaaacg tgattattat gaagtcttag ggctctcaaa aggagcatca
     9061 gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat
     9121 aaagaacctg atgcggaaga aaaattcaaa gaagtatcag aagcatatga gattttaagt
     9181 gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg caggcacaga tccaaattac
     9241 ggtgctggtg gagctggtgg cggatttggt ggattcggcg gcttctctgg tggcggcttc
     9301 ggtggttttg aagatatctt cgactctttc tttggaggag gcggtcggtc agtcgatcca
     9361 aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg
     9421 atttttggtg tagaaaaaga aatcaagtat aatcgggaag aaatctgtca tacttgtggt
     9481 gggaacggtg caaaaccagg cacacaacca actacttgtc ataaatgtca tggtgcaggt
     9541 acgattaatg tagaacgcca aacaccactt ggacgagtga tgagccgtca aacttgtgat
     9601 gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc caacctgtca tggctcagga
     9661 catgagaaga aagcacattc agttaaagtg aatgtaccag ctggtgtaga agaaggacaa
     9721 caaatgcgtt tagctggtca aggagaagct ggagaaaacg gcggaccatt tggtgatttg
     9781 tatgtggtct tccgtgtaga agaaagtgat atctttgatc gtgaaggttc tgaaatttac
     9841 tatgaattgc ctttaagttt tgtacaagca gctttggggg atgaagtcca agtaccaact
     9901 gtccatggag atgtgaaatt gaagatccct gctggaacac agacaggtac gaacttccgt
     9961 ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa
    10021 gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa gagaagcatt acgtgcattc
    10081 gctgaagctg gcggcatcaa agttgaagaa cagcaagaag acggtttctt cgataagttt
    10141 aaagatgctt ttggcgggaa aaagcggaag tagtttttct agtaaaaacg ctaagtcatt
    10201 aataattaaa aaccagacga acaatcatat gttcgcctgg tttttttgta gttgtcatgt
    10261 ttgtaccaac aaaaaagcca gctttagaat atgataatcg tcatagtttc taggggtata
    10321 accagtgaga gaatggaact tgttcaatcg atattgcaag gtgtttttat gaataaataa
    10381 ttcttctgag caatttttga tgcttccgtt atttttactg aagcatgcca aagtcttctt
    10441 gaacaaatgt acttcttctt ctgtcaggct tttcaaaata cgttttacat ataatgttcg
    10501 ttgatctgac gggattgctg gtaaaacgat cccgaggtct aaatcttcaa aaaagcggat
    10561 ctcgttcaca gattgatgga aagctatcca ttcgcaaact tgtttggcat gaaggaaaga
    10621 ttgcggtgtc tcttgctctg tataacaaag cgacccaatt ccaaagttta gctgtaactg
    10681 atacatttta ttaaactttg tctggatttt atcaagcaat gatagcaatg aggctctgga
    10741 aatccgattg actaggataa tgatttgttg tctatgtaca gtgagcaaag cctgtccgta
    10801 ataagagaag accctttcga gcaactccaa taaaggattg atccgttctt gacacaattg
    10861 ctgattgtca gaaagttgtc ccacaactac ataatatgga gaagaaaaat caagctcaat
    10921 taagtgaggg tggtatcgac tatgtacttg atatcatcaa tgaaccgatg gtactttctt
    10981 atgcgatgga acaggtagag cgattgcttg tttgtgcagg aaaacaagcg atgcggctga
    11041 tacgattagg tgagaaaata agaagataaa aatacacggt cccttcatgg cgaccgtgca
    11101 tttttggttt ttattaaata tttaatactt tattcaagaa atcttttgtc cgttcatttt
    11161 gcggattttc aaagatttgt tgaggcgttc catcttctaa aaagtttccg tcgtcaatga
    11221 acatgacgcg attggccact tctttagcga accccatttc gtgcgtaacg ataaccatcg
    11281 tcataccttg ttctgctaac tttttcatga cgttcaagac atcaccgacc atttcaggat
    11341 cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg gttcatcgct agagcacgag
    11401 caatagccac acgctgtttt tgtccaccag aaagcatttc aggatacata tcttttttat
    11461 cttctagacc aactgtctgc agtaatttga ttgctttttt ctctgcttct tctctagatt
    11521 ctttcttcag gtccatcggt gctaaaatga tgttttccaa aatagaaaga tgagggaata
    11581 ggttaaagtg ttggaatacc attccgatat gttgtctcac caagttgata tctgtgcttt
    11641 tatccatcaa gtgggcgccg tcaataataa tttcaccgtc agaaggttct tctaaacgat
    11701 tcaagcaacg caagaatgta ctttttcctg aacctgaagg gccaatgata cagacgacat
    11761 ctccttcgtt gatggataca ttgatgtcgt ttaatactgt gttatcgccg tattttttga
    11821 ctaaatgctc aactaagatt ttttctgcca tcttacttca ccttcttttc taaaactttt
    11881 gctaattttg tcaatgctgt gatcaaaatc aagtacatga tcgcaatgat caagtaaaca
    11941 tacgtacttt gtgttgtacg tgcaacaatg atcttaccag tttgcagcaa ttcgactaca
    12001 ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa cgaactgatt gacgaatgat
    12061 gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt gcattgtccg attataagaa
    12121 agacctaaac ttctggaagc ttccatttgt ccgacaggaa cggcgttgat cccgcctcga
    12181 acaatctctg cgatataagc acttgcgtta agagtcaagg tgataacacc agcaaggaag
    12241 tcaggaatcg taaatccaac gataccaggc aacccaaaga aaataaagaa cgcaagaacc
    12301 atcattggaa ttcctcggat gatatcgaca taaatactag caattgtgcg aagagcgcgt
    12361 attggtgcta cactgaataa tccaaaaatc acaccgatta ctatagccaa agcaaaggag
    12421 atcaaagtta ggagaattgt tctccaaagt cctttcaaca atgcttgata gttgttttgg
    12481 ataagtccaa gaaatgttga ttcatcagca gtggaagact cactggaagt ttctgaacca
    12541 tccgctacat attctttaat aatcttatcg tattcgcctg ttctttttaa ttctttcaat
    12601 ccttcattga acatattaag aagctcagga ttttgtcctt ttttaacggc aaatgcatat
    12661 tttccgccag attctttttt gatcggtgtt tccaattctt gtccttgttt gatcgcataa
    12721 ccgattaccg gataatcatc catcattgca tcgattgcgc caacttttaa cccgtcgtac
    12781 aattgatcag cagcatcaaa atatttgata gaatagccat attcagattc atggtcgctt
    12841 aaaaattcag cactttcggt accaactttt gctccgactg tttttccttt caaatcttca
    12901 taagacttga tcgaagtatc tccttttttg acagctaatt ggatcccgct atcaaagtaa
    12961 gaatcagaaa aatcatagct tttctttcgt tcatctgtta cagtcattcc ggcaatcatt
    13021 ccgtctgctt gtcctgattc caaagcttgc aaagcagcag agaaaccacg gtagtccatt
    13081 cggatgttaa atccttgcag tttggcgatg ttttccatca attttacatc gatgccttcg
    13141 tactcgccct ttgcgttttg aaattcaaat ggagcaaaag cagagtcact ggcaattacg
    13201 tatacgtctt ttttgggctc tattttttgc attgtctcgc ttgtgcttga ctctgccgtt
    13261 gtagtgttgt cagcttgtgc aacatgacca aacgcaaatg tcacaagtcc tgtcattaag
    13321 aaaacaaatg agtgaaataa atgctttctt ttcataatca acctcctaaa tttccaatat
    13381 tttattatta taggcgaaaa gtgctgaatc agacaagact gtaagctcga aaaaatgaaa
    13441 aaaatgcgag tttctaacat tttatgttat aaaacataac atgtataata agataaaaaa
    13501 gtggcgaaag tatgttcggt gtggtataat tttttagtaa ggaatgcctt tgctttcctt
    13561 ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa aggaggacga agaatgattg
    13621 agagagatac ctcccgacac tttgagcttg tctcgaaata ccaaccggct ggtgatcagc
    13681 ctgaagcgat taaccaatta gtggatggcg tagtcggcgg gaaaaaagca caaatcttat
    13741 taggtgcgac tggaacaggg aaaacatata cgatttccaa tcttattgag aaagtgaata
    13801 aaccaacact gattattgcc cataacaaaa cattggctgg tcagctgtac ggggaattca
    13861 aggaattttt ccctaacaat gctgttgaat attttgtttc ttattatgat tattatcagc
    13921 ctgaagctta tgtgccttca agcgatacgt atatcgaaaa agattcaagt gtaaatgatg
    13981 agatcgacaa attacgacat tcggctacta gttcattatt agaaagaaat gatgtgatcg
    14041 tcattgcatc cgtttcatgt atttttggat taggctcgcc ttttgagtac caaaaacaag
    14101 tagtttcgat tcgacaggga gcagaactag atcgcaatca gttgattcgt gacttagtca
    14161 gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg tcgttttcga gtgcgcggtg
    14221 atgtcgtgga gatttttccg gcttcaagag atgaacgtgc cttacgtgtg gagttttttg
    14281 gagatgaggt cgaacgtatc cgagaagtca atgcactgac aggtgaagtt ttaggagaaa
    14341 cggaacacgt agcaatcttc ccggcgactc actttgtgac gaatgatgaa catatggagc
    14401 atgccgtagc gaatatcaaa gcggaattag aacagcgttt aactgtcttg cgaaatgaaa
    14461 acaaacttct ggaagctcaa cgtttagaac agcgaaccaa ttacgatatt gaaatgatgt
    14521 tagaaatggg ctacacgtcg ggaattgaaa attattctcg gcatatggac ggacgtaaag
    14581 aaggtgaacc gccttatacg ctattggatt tctttcctga agattttttg atcgtggcgg
    14641 acgaatcgca cgtaaccatg cctcaaatca gaggaatgta taatggtgac cgagcgagaa
    14701 aacagatgct agtagattac ggtttccgtt tgccatctgc tttagacaat cggccacttc
    14761 ggttagaaga gtttgaaaaa catgtcaatc agatcattta tgtctctgcg acgccaggac
    14821 catatgaaca tgaacaaaca gataccgtta tccaacaaat catccgtccg acagggctgc
    14881 ttgacccagt gatcgaagtt cgtccaatca tgggacaaat cgatgatcta gtaggcgaaa
    14941 taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac gacattgacg aaaaaaatgg
    15001 cagaagacct cacagattat ttcaaagaat tagggatcaa agtcaaatat ttgcatagtg
    15061 atatcaaaac gctggaacga acagaaatca ttcgagatct tcgattaggt gaatttgatg
    15121 tgttagttgg aatcaatttg cttcgtgaag gattagacgt acctgaagtt tcccttgttg
    15181 ctatcttgga tgcggataaa gaaggctttt tacgtagtga acgttcctta gtccaaacga
    15241 ttggacgagc tgctcggaat gaagaaggta aagtaatcat gtacgcagac aaagtaacag
    15301 attctatgcg tctagcgatg gatgaaacgt caagacgtcg tacgattcag cagaagtaca
    15361 atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga aatccgagat ttgatttcta
    15421 ttacgaaaga atcggaagat gatacaaaag aagcggtaca agtctcgtac gaagaaatga
    15481 cgaaggaaga aaaagacacg ttattgatga aattagaaaa agaaatgaaa gatgcggcaa
    15541 aagctttgga ttttgaaaca gccgcgaatg tgcgagatat gattttagaa ttaaaagctt
    15601 caaagtagga ggacaacatg gctaacgata aaattacgat tcatggtgca cgtgcccata
    15661 acttgaaaaa tatagatgta acgattccac gagataaatt cgtggtcgtt acaggtttgt
    15721 ccggttctgg aaaaagttcc ctagcatttg atacgctgta cgctgaaggg cagcggagat
    15781 acgtagagag cctttctgct tatgctcgtc agtttttagg acaaatggat aagccggacg
    15841 tagatagcat cgacggattg agtccggcga tttctattga tcagaaaaca acaagtaaaa
    15901 atcctcggtc gacagtaggt actgtgacgg aaatcaatga ctatttacgc ttgttgtttg
    15961 cacgtgtagg acatccgatt tgtcccaacg atcatatcga gattaccagt caatcagtag
    16021 atcaaatggc agatcaagta atggctttgc ctgagcgcac aaagatccaa gtgttggcac
    16081 cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt cgaacgaatc caaaaagagg
    16141 gatacgtccg cgttcgagtc gatggtgaga tctatgattt aagtgaagtg cctgagctgg
    16201 agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg gatcattgtg aaagaaggga
    16261 ttcgttctcg cctatttgat tctttagagg cggccctgcg attagcagac ggctatgctc
    16321 tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga acactatgcc tgtccttatt
    16381 gcggatttac tgttggcgaa ctagaaccaa gactgttctc attcaatgcg ccttttggag
    16441 cttgtccaga ctgtgatggt ttaggtgtca aattagaagt cgatcaagat ttagtgattc
    16501 cagatagaac gaagacactt agtcaaggtg ctatcgttcc atggaatcca atcagctctc
    16561 agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt cggtatcgat ttagatacac
    16621 cattcgaaga attaccggaa gagcatcagg agatcatctt gaatgggaca gatgatcttt
    16681 tccatttcca ttatgaaaat gattttggtg gcgttcgcga tgtagaaacg actttcgaag
    16741 gggtattgaa gaatatcgag cgtcgttatc atgatacgaa cagtgatttc acgcgtgagc
    16801 aaatgcgtct ttatatgaca gaattaactt gtcagacatg taaaggttat cgattgaatc
    16861 cccaagctct atctgttcaa atcaatggaa cgaatatcgg gcaaacaaat gagttgtcta
    16921 ttcaaaaagc agtagatttc ttctcaaact taacgttatc tgaacaagag aaagtaattg
    16981 ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt cttggaaaat gtgggactag
    17041 actatctaac tttgagtcgc gcttccggaa cattatctgg gggagaagcg cagcgaatca
    17101 gattggctac acaaattgga tcgaatcttt ctggagtttt gtatatttta gatgaaccat
    17161 cgatcggatt gcatcaacgc gacaacgatc gactgattgg ctcattaaag aaaatgcgtg
    17221 atcttggcaa caccctcgtt gtagtcgaac atgatgaaga tacgatgtgg gcagcagatt
    17281 atttgattga cgtcggacct ggggccggag aacaaggcgg acaaatcgtg gctgcaggca
    17341 ctcctgaaga agtagctaat gatgaaaatt ctttgacagg gaaatatctt tcagggaaaa
    17401 aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg gaaagcaatc aagatcactg
    17461 gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt tccattagga gaatttgtag
    17521 cagtcacagg tgtgtctggt tctggaaaga gtacattggt caatagtatc ttaaagaaat
    17581 cgttagcgca aaaattaaat aagaattctg ctaagccagg taaattcaag acaatttccg
    17641 gctacgaaag tatcgaaaag atcatcgata tcgatcaaag cccaatcggc cggacgccga
    17701 gaagtaatcc agcgacttat acaagtgtat ttgatgatat ccgtgggtta tttgctcaaa
    17761 cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt tagtttcaac gtaaaaggcg
    17821 gtcgttgtga agcttgtcgc ggggatggaa ttattaagat cgaaatgcac tttttgcctg
    17881 atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata taattctgaa acattagaag
    17941 tgcattacaa aggaaaaagc attgctgata ttttggaaat gacagtagag gatgctgtag
    18001 aattcttcaa gcacattcca aagattcatc gcaaactgca aacgattgtt gatgttggct
    18061 taggttatgt gacgatgggg caaccagcaa cgacattgtc cggtggtgag gcacaacgga
    18121 tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa gaatttctat atactagatg
    18181 aaccaacgac aggacttcat agcgatgaca tcgcccgctt gttgcatgta ttacaaagat
    18241 tagtagatgc tggtaacaca gttttagtga ttgaacacaa tctagatgta atcaaaacag
    18301 cagattatat cattgattta ggaccagaag gtggagaagg tggaggaacg atccttgcga
    18361 ctggaacacc agaagaaatc attaacgtaa aagaaagtta tacaggtcac tatttgaaaa
    18421 aaataatggt ataaagagga tagatagctg agatagttaa aaaaacataa aaaaatgaat
    18481 ttttgcttgt tttattaata tcagtgttct agaatcatta catggtagtt ctttctacac
    18541 tgattggtat agataaaaac ttcctataag ttcaatccgt tcaaaaaaga attgagcaat
    18601 cggaggactt tcctttgatt gcttttttct ttatggtatg atattccttg tgtagttgga
    18661 aaagaaaaac aagagattat gagtaagtga gagataatga tgaacagtat tgaaaatgaa
    18721 gaatttatcc ctatattatt aggaagcgac atgaacgttt atggtatggc acgttctttt
    18781 aatgaagcat acggcaaaat ctgccaagca tatgcttcgg atcagttagc ccctacccgc
    18841 tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg ataaagaccc tgtatttatc
    18901 gagacgatgc ttcgtttggc aaaagaaaga tattctgata agagtaaaaa atatcttttg
    18961 attgcttgtg gtgatggata tgcagaattg atctcccaac ataaacaaga gctgtcagaa
    19021 tattttatct gtccatatat tgattattct ttatttgaaa ggctgatcaa taaagtaagc
    19081 ttttatgaag tttgtgaaga atatgatctt ccgtatccta aaacattgat cgtcagagaa
    19141 gaaatgttag taaatggaca tttggaacag gaattgcctt ttgaattccc agttgctttg
    19201 aaacctgcaa acagtgtgga gtatctttct gtccaatttg aaggaagaaa aaaagctttt
    19261 attttagaga ctagagaaga gttcgattta attttaggcc ggatatacga agccggttat
    19321 aaaagtgaaa tgattgtaca agattttatc ccgggtgatg atagtaatat gcgtgtgcta
    19381 aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt gccttggtca tccactatta
    19441 gaagatccta caccagcatc gattggtaat tacgttgtga tcatgccaga ttataacgaa
    19501 aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg aatatacagg ctttgccaat
    19561 ttcgatatga agtatgatcc tagagatgga gaatataaat tatttgaaat caatttgaga
    19621 caaggtcgta gcagtttctt tgtgacattg aacggcttga atctggctcg ttttgtcaca
    19681 gaagatcgag tgttcaacaa accctttgtt gaaacgacct atggaacgaa tcaatcagat
    19741 aaagcaagat tatggatggg tgtgcccaaa aaaatctttt tagaatatgc tcgggaaaat
    19801 gaagacaaaa aactagctga gcaaatgatc aaagaaaatc gttacggtac tactgtattt
    19861 tatgaaaaag accgatcaat caagcgctgg ttgttgatga aatatatgtt ccataactat
    19921 attccaagat tcaaaaagta ttttcatgtg aaagaaggct agttcatgga gaattttttc
    19981 agtattttag gcggaatggg cacgatggcg acagaaagtt ttgttcgctt gatcaaccac
    20041 cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg tcttattcaa tcatgcaaca
    20101 gttcctgatc gtacagctta tattttagat cgatcagaag aaaatccaat gccgtttctg
    20161 ctggatgata ttgagaaaca aaatttattg cgaccaaact tcattgtgtt gacttgtaac
    20221 accgcacact acttttttga agagttacaa gcggcaacgg atattcctat cttgcatatg
    20281 ccaagagaag cagcaaatga acttgtccgt cagcatacga cagggagagt agcgattcta
    20341 ggaacagaag gaagtatgaa agctggaatc tatgaacgcg aagtcaaaaa tcttggtttc
    20401 gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca attacctgat ctatcatgag
    20461 atcaaggaat cggattattt gaatcaagag ctctattacg aaatcttaga ggaagcggta
    20521 gagcgcctaa actgtgaaaa agtcatccta ggttgcacag aattatcttt gatgcatgaa
    20581 tttgctgaag ataatcatta cccagtgatc gatgctcaat ctattttagc ggatcgaacg
    20641 atcgaacgtg cattagctga aagaagcgag gcattggata cagcatcgga aaagtaaaaa
    20701 aattggattt ggggcaaata tcctaaaaga tatttggtcc aaatcttttt gtaaaagaaa
    20761 aagaaaactg gtaatgatac gcccgggatg atataatttt attatgcaca atgtgagagg
    20821 ggttcgtaaa tatgacagat aatttgcaat tagtcatcat aacaggtatg agtggtgcag
    20881 ggaaaacagt agctatccaa agttttgaag atatgggcta tttttgtatc gataatatgc
    20941 cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga atcaggtaaa gtcacaaaaa
    21001 tggctttagg agtggatttg cgttctcgtt ccttctttga agaaatccaa aacatgctga
    21061 tcgaaatcga aaatacgaac ttcatcgaca cttctatctt atttttagat gcttcagacg
    21121 aagaactagt ggctcgctac aaagaaacaa gacgagcaca tccaatggcg atggacggtt
    21181 tgatcactga agggatacgt aaagaacggg cgatactaga cgacttgaaa gcccaagctt
    21241 ccgtgatcat tgatacgaca acattgacac cacgacaatt acgtgaaaaa atcaatgagt
    21301 cattcaaatc aaagggagac caaggatttc ggatcgaatt tgtatcattt ggttttaaat
    21361 acgggttgcc gatcgattcg gatattgtga tggatgtgcg ttttttgccc aaccctcatt
    21421 atatcccgga actaagacca ttgaccggaa aagatcagcc agtctatgat tatgtgatgt
    21481 cctttgatga aacgcaatca ttctaccata aatttttgga tcttctggaa actgtattgc
    21541 ctggatacat taaagaagga aaaagcagct tgacagttgc aatcggctgt acaggcggtc
    21601 aacaccgttc tgtggcattg actgaacgtt tggcaaatgc tttagggaaa aactataaag
    21661 tcaatgtgac acatcgggac aaggataaaa ggaaagagac ggtgaaccgg tcgtgaaaat
    21721 gaaaacctac cggataagaa aaccaaagat cgtagtggtc ggcggtggca ctggtctgcc
    21781 agttatcctg aaaagtttac gaaatcaagg agcagatatc acagcagtag tgactgttgc
    21841 agatgatggt ggaagcagcg gtgcaatcag agaatcgatc gctatggctc ctcctggtga
    21901 cttgagaaat gtcttagtgg cgttatcgga tatgccacaa ttctatgaag atattttcca
    21961 ataccgtttc aaaaaggaag atcagttttt agccaatcat acgatcggta atctgatcat
    22021 tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg attcaattat tagcaaaaat
    22081 gatgcatgta gatggacatg tttatccttc ttcagaaact cctttgacct tgcatgctgt
    22141 ttttaaagac ggaacgactg cagtaggaga atctaaaatc gcgatcaacc gtaaaaccat
    22201 tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa gccaaagcgg ctcgaaaagt
    22261 cgtttcagca attatggacg ccgacatggt cgttttggga cccggaagtt tattcaccag
    22321 tatccttcca aatttagtga taccagaaat cggcgaagca atgctcaaga caactgctga
    22381 aaccgtctat atctgtaata tcatgaccca aaaaggggaa acggaacatt tcacagatgc
    22441 cgatcatata cgtgttttac acaagcattt gaatcagcct tttatcgata cagtattggt
    22501 caatacggaa aaagtgcctg aagattatat ggacccagag atctatgatg agtatctagt
    22561 acaagtcgca cacgacttca acggattgag aaatgaagga tgtcgagtga tttccaccga
    22621 ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga gacaaagtag tagaagaatt
    22681 gttccgttta gttttcggtt caaaatatta agcaatttat tgttggagaa aggaggagaa
    22741 taggatgtca tttgctgcag atgtgaagaa agaactaact ggtttggaag ttcatcgaga
    22801 acatgcgaaa gcggaattag ctgctttgct gcgaatgaac ggctctttag gtattgtgaa
    22861 ccagcagttt attttgaacg tccaaacaga aaatgctgca attgccagac gtatctattc
    22921 tcttttgaaa gatcattatc aggtacgaag tgagctactc gttcgtaaaa aaatgaaatt
    22981 aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc acaaaagatg tgttgatgga
    23041 tcttgatatc atggatggta tgatgttcaa tggacacgtt tcggacgaga ttatgggaaa
    23101 ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg gcctcaggtt cagtcaataa
    23161 tccggaaacg agtcgttatc atttggaaat attttctatt tatgaacagc ataaccaaga
    23221 catttgcgat atgttaaatt attacggtct acatgctcga acgcttgaac ggcgcaatgg
    23281 ctatatttcg tatttaaaag gagcagaaaa aattgctgat ttcttaacat tgattggtgc
    23341 aaccaactca atgctccgat ttgaagatgt tcgaatcgta cgggatatga gaaattcggt
    23401 caatcgtcta gtcaattgtg agacagcaaa tctgaacaag acgattgatg cggcttctaa
    23461 acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt ctacatgccc ttcctgaaaa
    23521 acttcaggaa atcgcagaac ttcggcttca acatccagag gtcagcttga aagaattagg
    23581 ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt aatcaccgaa ttcgcaaaat
    23641 aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa tccaaagaaa aaactgatac
    23701 atgaagcagg agatgcgaat gtatcagttt tttttaaaag ctatacttgc ttttcttccg
    23761 tcaaactata attccaactt tactggagta acataatgtt gaaaggcttc tattgttttt
    23821 taatacaatc gtataaaaag cggggagaca actgaatgaa gattttagtt ttatacataa
    23881 acttttgctt aatatagaag tcctgatata gataggatca agtatttatc tataactgga
    23941 gattaattaa aaactggaag gaactgctcg atcaaccttg agaactgttt ttcttacttc
    24001 tggtcaagat tctcgtggtt ggtggataag gatggttgat aagttcattg gattagctag
    24061 tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca aggggatgga gtgatttatt
    24121 cagtgataat cataagttca taaatagtaa ttgggatgta tggttggtat acgttatacc
    24181 tgaacttgaa ctatcaagat ttggtttaga atttgagaat cgatgtttat aaagggaggg
    24241 caaaaaatgt gcttttttac aacttttact atcattatga ttgcagtact ttttattttt
    24301 ctagatatag caaagcgaaa tacagcattt ttattatatc gggttttact tcgagcaggt
    24361 ctcataacat ttatatcgat cgttggattt tttctattca cagttattgt atttatttgg
    24421 agaactacag caccaccatt gccagagatt acatatggag aatttccgtt tagtcttgaa
    24481 tatgaaatag atgaggaaag aattttgatt gaagatacgg tgattgcaga atttatagga
    24541 agttttagag ggaatgtgac aactcaacct agacgaagtt ggagtataaa acttttaaat
    24601 aatagtggag agttgaaagc atttcattat gaattcttaa taatagaaag tgaagatgta
    24661 acaatcactt taagtagtgg ctctgctgga tattttatga atgatggtgg aagcaagcca
    24721 cagctcacag tccgtgatcg tattgcaggt tttatgcatt tttctcacta tgacttagaa
    24781 gaagctcgta aaatacttgc agaatatggg atcacattaa tcaattggga atatgcgcca
    24841 ccgataagaa attttttcaa tgattattat tagtcttata aaaaacagaa tgtaaaattt
    24901 atgtgcgcga gtatttagtt ttccattttt tgtttgatag atatatagca ttgttgaagc
    24961 atgggatagt ggtgccaatt ttatcaatga ggttggtcag gcagttaaca taattggtga
    25021 tgctataaat ccatttaatt ggaattggag gaattaggtg tggaaacagt gagattatgg
    25081 atattcatga aaaattttcg ttcggctttg agatatgtta ttctggaatc aaaatcaggt
    25141 tattatctga ttgacacaga tcactatact ttttggggag gctacctttt tccgattttt
    25201 aattggataa tcccccataa ggttatgaaa attaacttgc ctgcagaaga aataaataga
    25261 ttaatactca acaaagattt acaaaggaaa agagagaaag aacattctaa tgttgcttgg
    25321 ataggtccag catttgcagt agtgacttct tcaattgtcg gtcctatgct agagagtatt
    25381 tatagtgatt tttctattgg aatacgttat ttatttgtta ctttaagtat agctgttctt
    25441 ttgtgcataa agggttatgt tagcaagaag aataaagata ttttaacact aatcggtgta
    25501 aacaatttat ctagtactaa aattatactg tttcctgaat ctataaaaga aggatcaagg
    25561 ttgttgataa tttttataat gcttatactg ttttctgttt tatttggggt gatgtttata
//
LOCUS       DASJOX010000027        25561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJOX010000027 DASJOX010000000
VERSION     DASJOX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.027"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..2214
                     /locus_tag="U4A12_001066"
     CDS             154..2214
                     /locus_tag="U4A12_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN1773464.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2297..2866
                     /locus_tag="U4A12_001067"
     CDS             2297..2866
                     /locus_tag="U4A12_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773465.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3041..3847)
                     /locus_tag="U4A12_001068"
     CDS             complement(3041..3847)
                     /locus_tag="U4A12_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773466.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3985..5499
                     /locus_tag="U4A12_001069"
     CDS             3985..5499
                     /locus_tag="U4A12_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN1773467.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5902..6885)
                     /locus_tag="U4A12_001070"
     CDS             complement(5902..6885)
                     /locus_tag="U4A12_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN1773468.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6890..7537)
                     /locus_tag="U4A12_001071"
     CDS             complement(6890..7537)
                     /locus_tag="U4A12_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1773469.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7622..8254)
                     /locus_tag="U4A12_001072"
     CDS             complement(7622..8254)
                     /locus_tag="U4A12_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1773470.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8270..8821)
                     /gene="coaC"
                     /locus_tag="U4A12_001073"
     CDS             complement(8270..8821)
                     /gene="coaC"
                     /locus_tag="U4A12_001073"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN1773471.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8818..9555)
                     /locus_tag="U4A12_001074"
     CDS             complement(8818..9555)
                     /locus_tag="U4A12_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN1773472.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9552..10541)
                     /locus_tag="U4A12_001075"
     CDS             complement(9552..10541)
                     /locus_tag="U4A12_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1773473.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10799..11341
                     /locus_tag="U4A12_001076"
     CDS             10799..11341
                     /locus_tag="U4A12_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1773474.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11358..12443
                     /locus_tag="U4A12_001077"
     CDS             11358..12443
                     /locus_tag="U4A12_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773475.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12455..13261
                     /locus_tag="U4A12_001078"
     CDS             12455..13261
                     /locus_tag="U4A12_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773476.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13262..14089
                     /locus_tag="U4A12_001079"
     CDS             13262..14089
                     /locus_tag="U4A12_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773477.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14086..15159
                     /locus_tag="U4A12_001080"
     CDS             14086..15159
                     /locus_tag="U4A12_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1773478.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15587..18283
                     /locus_tag="U4A12_001081"
     CDS             15587..18283
                     /locus_tag="U4A12_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1773479.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18360..18848
                     /locus_tag="U4A12_001082"
     CDS             18360..18848
                     /locus_tag="U4A12_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1773480.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18860..19354
                     /locus_tag="U4A12_001083"
     CDS             18860..19354
                     /locus_tag="U4A12_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1773481.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19375..20184
                     /locus_tag="U4A12_001084"
     CDS             19375..20184
                     /locus_tag="U4A12_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1773482.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20181..21002
                     /locus_tag="U4A12_001085"
     CDS             20181..21002
                     /locus_tag="U4A12_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1773483.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21053..23152
                     /locus_tag="U4A12_001086"
     CDS             21053..23152
                     /locus_tag="U4A12_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN1773484.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23149..24267
                     /locus_tag="U4A12_001087"
     CDS             23149..24267
                     /locus_tag="U4A12_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1773485.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24306..25184
                     /locus_tag="U4A12_001088"
     CDS             24306..25184
                     /locus_tag="U4A12_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1773486.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25330..25527
                     /locus_tag="U4A12_001089"
     CDS             25330..25527
                     /locus_tag="U4A12_001089"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1773487.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8074 a         4451 c         5411 g         7625 t
ORIGIN      
        1 taagttatta ttccgatttt cttgaaacaa taaaaaatca aaaaaaatac tctcctaata
       61 gtccatcttc ttttgatttt atttatataa tgaaatcatc ttatgaaaat tactacttca
      121 tgtttagaca agagtaggga ggactaagaa taaatgtgtt caaaaagacg ttcaaaacaa
      181 ctaaataaac acgcttgtcg tttagataag gaacggtcgt gttctgaaaa acactacgtt
      241 aagtttatga tgcgcacgat gctgatcagt ttatctgtag gtgtctatat gcatgtaccc
      301 gttcaggcaa cgacacaaac catcaaggtc gtacgtacct ctgtttctga gaccactgat
      361 tcccctatca ctattactca tactttcact gatgcaaatg gaaaacagct acttcctgca
      421 ttgacttcct ataataaagt cagttatcct ccaacgataa accactactg gttaattcct
      481 aacaaaacat tatggacatc ggaaaccgga agcagtgtgc cgctttctat cttttccgat
      541 atgactgatc ctacaaaagg tgatcagtta aaacagatca ctgatatttt gaatcatgct
      601 ttgacagata taaaggacgg tggaagcgca caagtttctt atgtatatga aaaagattat
      661 tcacagtttg aaagaaagaa aatcgatgtt ctggcaggga caaaagtttc gatatctgat
      721 ctggtagaaa ggctgaagaa cgcagatgga acacaaggag atatgtccaa agtaatttca
      781 aacaatggga atatcgtgga tacgactaat acaggaacat ctactatttc cttgtcctat
      841 tttgatccaa ttgctttaaa aacaatgact gcatctgctg tgatccatgt tcgtgctgat
      901 caaacagcta tcaaaggaaa agatcttcac ttaagaacag gagagagtta cgcccctaaa
      961 gatcttgttg aatatgtaac aaaaagcgat ggaacttcag gaaataaaga agaaatcaaa
     1021 atgaacggga cagtagatac tacaactccg ggagaatatc ccgttgtatt aacttatata
     1081 gactcgaaaa catcaaatgt ggcagaaaca acagctgtag tttatgtaga agcatctgct
     1141 gcttcatcga ctgtcatcac tcattttata gatagtcaaa caaataaaga acttcaagca
     1201 gaaacacata tgagtagtgt aaatcgtatt cctgatttgc gcctacctgc tgaattttct
     1261 gatcgagtgc caaatataga tttatcgact tatgaacaaa atgggaaagt cacttctatt
     1321 tctgattgga tgcaagcaaa cggattagac agaaaaagtc actggtcgga gatccttgaa
     1381 gtacttcact cagaactgat gaataatagg aagcaatcag gaggggaaat tacctttact
     1441 tatgtttata taccagacaa tcgttcatta gaaggtcatg atcttattgt tcatccaaac
     1501 gaaaaaataa acaaagaaga gttgatccaa tcagcaacaa attccttagg taaacccgta
     1561 ccgttaaagg atgtatcggt tacaatcaat gaagtaccat tggaggatgg ttatttgacc
     1621 aaacaaacag gtgattttac gattatgtat agttatcatg acccgtattc actccaagaa
     1681 ctccatgctc agtcactatt acatgtttta ccattaaaca aacagataga tcaaaaagaa
     1741 caaaaaagac gagaagaaag cttgtcagaa aaaaaacaac aaatggctca tgcagtttct
     1801 ccaacaggga aagtaaaaca aacaagaagc ctagagttag caagaatatc tagaaaaatc
     1861 tcagaatcta ataaaacgat aaggcaagaa gctcatttgg ccccacaagt ttctggattg
     1921 gaaaatgaaa ttttagagcc taaaataaaa caacaatcag aaaaagaatt tgttgctaaa
     1981 cagtcggaag atcagaagga atcagatgac cagaaggaaa cgaagaaaaa acgcttgtca
     2041 actattgatt ccaccgatga gcatctgcag ccgcaagtac cacttcctag tccgggaaca
     2101 cccgcacctg gtggaggtat tccttcttct gctttaggag atttcatgcg aggtatggca
     2161 ggaacatggg tatacgctaa tcgaaatgaa tttgacgcag atgactggtt ctgatttata
     2221 tctctatctg ttatagtcaa ataagtagct gagtcatatg ttatttggca taatggagac
     2281 gaggagggat agaaaaatgt tagataaaac gttaccttac gcagaaatct ggatgacaag
     2341 accacttcaa caaaatgttc caatcacgcc tttagcggaa ggttttgaat ttcgatttta
     2401 tcaaaaagaa gatgaatctg cttgggcgga gattgagact tccgttgggg aatttcaaac
     2461 gacagatgaa gcacttgagt attttcaaca aaactttgct ccttatcctg aagaacttga
     2521 aagaaggatg ttttttgtag agacaaaaaa aggagaaaaa gtagcaacct gtaccgcttg
     2581 gcgaaaaaaa tcaagagagc aagtgtatcc ggtttttcat tggttagcag tgaaaccaga
     2641 ttaccaagga aaagggctcg caaaagcatt gactgccaga gtcttgcaag aatttcctac
     2701 cctccatacg gacggaccaa tctatcttca cacacagaca tggagccatc aggcgattgc
     2761 tctttataaa aaaatgggtt ttacttttat tgctgaaaat ctagacggca cacctaatcc
     2821 agattatgaa aaagttatgc aggttttaag taaattagat aaataacgaa atggtgaaat
     2881 aagtcctcag tattgggata aaaaacgaaa tagtcattcc tcatatctta cttttttgag
     2941 ttaatactat cggataacta atggcatgcc atacattcaa aaaagaaggg gcagtgacaa
     3001 aagtattgtc actgcccctt ctcttgtttt ttagcatgtt ttatcgtttg atttttttag
     3061 gaataaaatt cttgatgaac gtaaattttt ggtaatcgtt tcgactatag aggtaaccaa
     3121 atacactgaa aacaactgct ccaataaaat taacaaataa atccttcata gtatccatga
     3181 ttcccacgtc taagtaacca ttttggatga ttgttttatc tgttttacca tctgcatttt
     3241 gactttcaat gattgtccga gtgatatctg gaacatgata gacagtgttt ccgtctttac
     3301 ttaactccac actattgatt tgctggatga tcgtatcttt ttgcatatcg aattgcagta
     3361 atttatctgc tgaaaattcc acaaactccc acataacacc aactgtcatc gaaaaacaga
     3421 aggaaacaac tgctagtaat aaaggagtca agttcatggt ttttgtattt ttattgagca
     3481 aataaacaag ggaaaagccg acacccccag ctaaaaatcc gctgaatgca tgtagaaaag
     3541 tatcccacat tgaaaaatag ccataaaaat cacttaactc tcctaaaact gttgaactaa
     3601 aaataaaaga taacaaaatg atttctagca tatttggaat ctgtatcttt aatatctttt
     3661 ccagaaaata agggatttca aataaaatta atgtcagtaa taaaagcatt gtatttcccc
     3721 agtgttggtt ccagacctga tccactagca taataacgac gatgatacgt aataaaatat
     3781 agattccgtt tgtgattcta cctgcagttt ttgtactgtt cgtaaaaaag gttttcaaat
     3841 gattcataaa ataactcctt catgtatatc cttaattata ctaagaagcg tgtgtttacc
     3901 aaagctatat gacttaaaga aaaacagagt tgatattgtg aaaaaaatca cttaatatag
     3961 tataaggaga aaagggggat acaaatgaaa aaattagttg ctggtttatt aacagccgtt
     4021 gcacttcttg ctatgagcgc atgcggcgca gatcagaaaa atacagcgca gacaagttct
     4081 agtacgaagg aatctgccga agtatcatca ggcgcttctg aacaagtata tacagatcca
     4141 aaagaaatga aagaatctta tgatgtcgtg attattggag ctggtggtgc agggatggca
     4201 gcagctatcg aagcaaaaga tgctgggttg aatccagtaa ttttggaaaa gatgcctgtt
     4261 gccggaggaa acacatctaa atcatcaagc ggtatgaatg cttcagaaac aaaatttcaa
     4321 aaagaagaag gaatcaaaga ctcgaatgat gccttctatg aagaaacgct aaaaggtggt
     4381 ggaggaacga atgataaaga aatgcttcga tactttgtcg atcactctgc tgatgcggtc
     4441 gattggctag atcaaaacgg tatcaaactg gacaacttaa cgatcactgg agggatgagt
     4501 gaaaagagga cacatcgtcc gtctgatggc tcggctattg gtggatactt agtggagggg
     4561 ttattgaata acgtccatga gcgggaaatc cctatttttg tcaacgcaga tgtgacagac
     4621 attaagaaaa atggggaaac ggtcaatgaa gtgactgtac atgttcaagg agaagaacca
     4681 aaagaggtca ccggaaaagc aatcgtagtt accacaggcg gttttggtgc cagcaaagaa
     4741 tttattgaaa aatatcgtcc cgatttagct gattatatta cgacaaatca agaaggatca
     4801 actggagatg gtatcaccat gattgaaaaa ttaggtggac aaaccgttga tatggataaa
     4861 atacaaattc atccaactgt gcagcaggat gagggggtct tgattggtga ggttgttcgc
     4921 ggagaaggag caatcttggt cgatcaagag gggaaaagat ttgtcaatga aatggataca
     4981 agggataaag tatctgcagc aattacagca ttgcctgaaa aatcagctta tttgattttt
     5041 gaccaaggcg tgcgtgatcg tgccacagct attgaatttt atgaccaaaa aggatatgtc
     5101 acaactggaa agacaatgga agaattagca gaaaaaatcg atttaccaaa agaaacacta
     5161 gcaaaaacag ttgatggatg gaatacggat gttgcggaga aaaaagatgc tcaattcaac
     5221 cgtgccacag ccatggaaca tgacttatcg tctccgaatt attatgcgat caaaatcgca
     5281 ccaggaatcc attacacgat gggcggtgtg aaaatcaata cgaacactga agtattagat
     5341 aaagaaaacc agccaattag ggggttatat gctgctggtg aagtcacagg tggcttacat
     5401 ggaaacaacc ggatcggtgg aaattcagtt gcggagatca ttatttttgg gcgacaagcg
     5461 ggcatacaag cagctcgatt tgcttcggaa aatgaatgaa atagaacgta aataaaaaga
     5521 ggttgtgaaa tcagcgtttt gagctaatac cgagggtcag ctgattgaac actgtttagt
     5581 gaagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc
     5641 ttcttatgtt tttaacttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt
     5701 tattcggaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     5761 gtataaacgg cggaagcaga agaaattcct tcggaaataa gctgaaattc acaaaaattt
     5821 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
     5881 tcttttttga tcagcatgat tttattcagt tatcttcaaa atgatttttc cgatgttttt
     5941 gtttgcttcc atatattcat gcgcttgttt gactgcgctg aattggaatg tacggtcaat
     6001 gattggccga atcttgccat tttctagaaa aggcccagct ttttgcataa aagtttgtgc
     6061 gagctctgct ttatactgat cacttctagg tgtcaataat gtaccaagca gttggacacg
     6121 tttcccaatc aatacaccaa ggtctatctc aggcacgatg gtcccaccta gcataccaat
     6181 caagacccaa cgtccatcaa cggctataga agctaaattc ttttcccagt aagaagcgcc
     6241 gatgaaatcc aaaataacat cgactccccg accttcagtg atttttcgta cctcttcttg
     6301 aaagtcttgt tttttatagt tgatcaattc atctgcacca agttctttac aaaaggcgag
     6361 tttttcttct gatcctgcag taacgatgat tttcgcgtga gaaagctgtc tagctaattg
     6421 gatcgctgct gttcccacac cacttgctcc tgcatggatc aagacagttt cgccttcttg
     6481 taattttcca agccagtaaa gtgtttgata agcagtcaaa aaaacttctg agattccagc
     6541 agcagaaaca tagtctagcg tatcagggag aaggattgca cgatcggcag gcatcacagc
     6601 atattcggca taaccaccta gattgactaa tccgtatact tttgtcccag gagtaaaagt
     6661 ggagatatca cttttattct caactacctc tccagctact tcgactccca aaatagggta
     6721 gggaggtttc aaagagactg attctctttt cataatatct gttcgattga cagctgctgt
     6781 atggactttg atcagtaatt cgccttgttt tggtttagga acaggcactt caatttgttc
     6841 taatacatct acagaaccag gatgcttgat tgaaatggca cgcatggatc tattcctcct
     6901 ttgcccattc ttggaatgct gtattcaact ctttgctacc ttcagaaagt actagagtcg
     6961 tacgagaaat atccgtttct tccactagag cggcaagtac agcagcaact gtagagcgag
     7021 tgatcgtttt tgatgccttc tcattaggct tagttaattg aatggaagta acttcttcat
     7081 cgttagtcaa tgtgactggt tgaagaatga caaaatctaa gttagttcgt ttcagccatt
     7141 catctgcata atgtttagta atataataat ctgtcatgga ctcaggccat tttgctggtt
     7201 catctgcata aacagcactg accatcagat agcgagagac atgcgcattt tcagctgcga
     7261 tcactgtttt gattgcgcca tccaagtcta cttgcagaag attctttcct tgagagccgg
     7321 cagcaaaaat caatacatcg atgcctttga atgcttcggt cattttttcc acggaccaag
     7381 ttaaatcaaa agagacatac gaaacattct ctttttttac taatgtttgg gaagcaacat
     7441 ctctcacccc tgcaaaaatt tcatgggaac ttggagctaa atcttttatc aggtgtcggc
     7501 caatttgtcc gtttgcacct acaacaaaaa ttttcatttg gatcatcctt tcgacgtcaa
     7561 atctttccat tcaatagtaa caccaaaaga gaaaaaaatc agagatcggc tacgatgact
     7621 atcagttagt aagagaaggg tgcttgaagt atcttaacaa gacattgcat actgctgcag
     7681 tcgcaagacc tgcgacgatt ccttcaggta taccacttgt aaaaataacc gctagaatag
     7741 ctgaataaac agcatttaaa tttgttccga tgactttggc ataatcttgc tggaataaaa
     7801 agtagatcat attcatcact aaaatcgtat tggtcaatga accggaaagt ccagctaaga
     7861 ataaagaaac ggaatctgct ttttgtttca ttagtttgcg cagaccttta taaacgaaat
     7921 aaggaaccac cccaatcaag atgcgaggaa taaacgcgat cagcaaggct ttccagcttc
     7981 catgttctgt tccgatgact gggataaacg gagaaaagac aaaagacaaa ggtgtttgga
     8041 taactgtgct acggatcatg ctgatcagtc caaatgtacc gccaagaaaa gcacctagtc
     8101 ttggtcctaa aacgatcgaa gcaatgatta caggaatatg catggttgtt gcattgattg
     8161 gaccgatagg gataaaacct aaaggcgtaa cagctaataa aatcaaaata gctaaaaaca
     8221 tcgcagttaa tgtaaagttt tttgtatttt tcataagtac tccttttttc taagcatttc
     8281 tttcattaag agtcagcatg acctgttcga tgatcgtctg aatatcagct aaggcacctt
     8341 ttccaaaatc tccacaagct aaaagagatt ctcttggttc aatttcctga tagccgacag
     8401 atctcaatgt ttccagatta cgctgagtga tcgggttttg atacatattc gtgttcatgg
     8461 caggggcaat caattttggt gttcccactg gaagcgccat tgcgactgtt gaaagaagat
     8521 cgtctgcaat gccgtttgct agcttgccga taatgtttgc tgtagcaggc gcgatgagaa
     8581 acaagtcagc ttgttttgct agttcgatat gattgattac ctgcggattt gattcttgca
     8641 tgacgtctgt atggactgcg cgcttcgaca gagattgcaa ggttaatggc gtgataaaat
     8701 gtgtgctgct tttagacata atcacatcta cctgggcacc taatttgacg aattgacttg
     8761 taagatcagc gcttttgtaa gcagaaatac ttccagtaac gcctagtaag atctttttca
     8821 ttttgattcc tccagtctaa ttttctcagt aatcaatgca gcaatctcag atttactttg
     8881 agcttcttcc actgttccat ctttagataa gagatagccg tggtgtgtct cgccatggat
     8941 ttccgtcaaa tcatttgcga aaataaaatc agttttgtta tttatcagac tattttttgc
     9001 gactgccaga agagattcct ttggtacatc tactaatagt ttgaaaccaa tgatttttgc
     9061 ttttggctgc cattcacgta gataagaaat gatttttggt gttttcttca aaacgagcac
     9121 aagatgctcg gtgttagaag agatctttga aacagtggta tctgtctgtt cattttccgc
     9181 aaaccaattg tccagttctt cttgcgttga agaagtgggt aggttttttg ccagctgttc
     9241 ttctgaaaaa ctaaaagcag gagtaaaatc actgactgcc atactgtgga taatcgcatc
     9301 gtagtgttct tttttcgtta gttcttctaa ttgtaaaaat aagtccagtg cagattcgat
     9361 cgtataaatc gttatattgg agtgtctttt cggtttcagt gcttgtttcg tagtcacgta
     9421 atcgattgtg gtatccggat tggcagctaa ataatcagct aatgcttgcc ctaatcgtcc
     9481 tgtcgaatga tttgtaatag cacgtacttg atcgattttt tcagtcgttc ctccagctgt
     9541 gattagaatc ttcatttatg gtcacctaca tttactagtg ttcttccttg gtgtgttcca
     9601 gcaagtagtt tagatgctgt ttcaggcaat tgatccagag taatctcttg gacgaccagt
     9661 tcatctgcaa tctgcaaatc cgccatctgt tgccataaag aaagacgttg atctattggt
     9721 acattgacag aatcaattcc aatcaactgg atgtttcgca gaataaaagg caagacagtc
     9781 gcgtttagtt tgattccccc agcattgcca cataaagcta ctgcaccgtt ataagagatc
     9841 aatggaagaa gactgcttag ctgctcgcca cctactgtgt cgatgacata gtcgatttgc
     9901 tgttttccta aagcttttgt tgtctctggc agaaattcat caggtcgtac gacttgcaaa
     9961 gcacctagac tttttagcca tgtgacagac tcttcttttc tggaaaaggc aatgattgac
    10021 tggaatccta gtttatgtaa cagggctatc gctgtactgc ctacaccgcc agaagcacca
    10081 gtaacgacaa cttttttatc ggaggtcata ccttgacgga ttaaagcgtt cacacacaac
    10141 atagcagtaa accctgctgt accaaggatc atcgcttgac gcagcgtcat attttttggt
    10201 agaggaacta accagtctcc tggtactttt tgatattggc tataaccgcc tggatgggtg
    10261 acgccaagcc caaaaccagt agcaagtaca agatcttctt ttgaaaaacg tggattgtta
    10321 ctttggacga cagttccgga taaatcaatc cctggagtca tcggatatgt tcgtatcacc
    10381 tgaccagact cgctcattgc caatgcatct ttataattga catcagaata ggcaattttt
    10441 accaagactt cattttcagc caactcagta aatggcagat cactgattgc tgtatggaaa
    10501 acaggttcct ttattacctg aaaagatcga aaagcgttca ttcgtgagac actcctttta
    10561 aaattttctt ttatcatacc ataaagccta ctcttttatt cttgttcttc cgtaaattta
    10621 gacaaaaatt aggtgaaaaa taaagaatca aacaactaga atttattcca aaagataaag
    10681 aaatattcag caaaaaaaat tttacaaagg gttgcctttt tcaataaatt tcgtatacta
    10741 atacgagtgg tagtaaacag gaagtttact attagaaaac ttcgattcgg aggaaattat
    10801 ggaaattggt gagaagttaa gaaatttgcg catccaaaag aatttgacac aagaagaact
    10861 aggcgaacgc acagatttgt caaaagggta tatttcccag ttggaacgag atttaagttc
    10921 accatcaatg gaaacattct tttctattct tgaagtctta ggtgtgactc cagaagaatt
    10981 ttttcatcaa gaagcagcga atctgcaagt ggtttattca aaagaagatc atacggttta
    11041 tttcgatgaa gaaaacgggt atgaattgga atggctcgtg gctgattcga atgaaaaaga
    11101 aatggagcct gtcttgctga cttttgaagg tgcaggagaa tataagacgt ttgaaccttc
    11161 attatctgaa acgtttattt ttgtattaga aggggaatta gagttaagct taggagagac
    11221 ggtctatgtg gcaaaagcag gacagtcgat ctattatcaa gcgacaaaac aacatcaatt
    11281 aaggaatcat ctcaaaaaac gaacaaaagt attgattgtt gcaacgcaat catatttgta
    11341 atataggggg taaaaacgtg gggaaaccaa tcatttcatt tgatcaagta gtgaaacgct
    11401 atgatgatga aacagtatta aaaaaagtaa gttttgagat cgaacaagga aaattttata
    11461 cactgcttgg tccttccggc tgtggtaaaa caacgatcct acgtattata gctggattca
    11521 ccgaagctac agaaggggac atctattttg aagggaaacg gttgaatgat ttgccagcta
    11581 ataagcgaca agtaaataca gtcttccaag attatgcact ttttcctcat atgaatgttt
    11641 ttgataacgt tgcttttgga ttgaagatca agaaaatgtc caaatcagat atcgaaaaga
    11701 aggtaaaaga tgctttgcgt atggtccagc ttcctggata cgaaaaacgg gagattagtg
    11761 aaatgtctgg tggtcaaaga cagcgtgtag cgattgcacg agcaattgta aatgaaccga
    11821 aagtattgct attagatgaa ccgttatcgg cgcttgattt gaaactacgt acagatatgc
    11881 agtatgaact gcgggatctg caacaacgat taggaatcac gtttattttt gttactcatg
    11941 accaagaaga agcgttggca atgagtgatg agatttttgt catgaataaa gggaaaatcg
    12001 tccaaagtgg tacacccgta gatatttatg atgagccaat caatcatttc gttgctgatt
    12061 ttgttgggga gagcaatatc gtggacggtg taatgatcga agataatcta gttgagtttg
    12121 ttggtaagca atttgaatgt gtcgatggcg ggatgcgtct aaatgaacca gttgaagttg
    12181 ttttacgccc agaggatttg acgatcacga caccagataa aggaaaacta gtcgtaacgg
    12241 tcgatactca gctgtttcgt ggtgtccatt atgaaatcat ctgttatgat gaacagcaaa
    12301 atgaatggat ggtccattca acgaaaaaag ctaaagaagg aagcaaagta ggattggcct
    12361 ttgagccaga agatatccat gtcatgcgtt tcaacgagtc tgaagaagaa ttcgatgcac
    12421 gtcttgacag ctacgaagaa taggggggac aagaatgaaa acaatgcgca ggatctattc
    12481 cgtcccttat attttgtggc tcggtttatt cgtgatcgcg cctgtattaa tgatcattta
    12541 tcaatctttt tttgatatga atgggcaatt cacgctgaat aattatctat cttattttac
    12601 gtcggggaca tcgctttcta tgacgatcaa ttctgtatgg tatgcatttt tgatcacgtt
    12661 tttcacgctg atcatcagtt acccgactgc ttatttttta acaaaactga agcacaagca
    12721 attatggctg atgctgatta ttttgcctac atgggtgaat ctgctgctta aagcttacgc
    12781 atttattggg attttcagtg tgcacggcag cgtgaatcat ttctttgatt ttatcgggat
    12841 cgggccgaaa cagatattat tcacagactt cagttttctg ttcgtggcaa cgtatatcga
    12901 gattccattt atgattatgc caattttcaa tgcattggaa gagatgaatc cttctttgat
    12961 cagtgctagt agagatttag gcgcgaatag ctttgagacc ttccgacgag tgatttttcc
    13021 gttatcatta aatggtgtga aaagtggtgt ccaagcagta tttattcctt ctctttcttt
    13081 attcatgctg actcgtctga ttggcggaaa tcgggtgatc actttaggaa cggctatcga
    13141 acagcatttc ttggttacac aaaactgggg gatgggttct acaatcggtg tgattttaat
    13201 cattgctatg ttcatcgtga tgctcctgac aggcgagaag aaaaaaggag gtcgctcata
    13261 aatgaaaaaa tttaaatggt cgtatcttta tttagggatc gtctttttat tcttatatgc
    13321 accgatcttc tatctgattt tttattcctt caatgaagga ggaacgatga acagctttac
    13381 aggatttact tgggaaaatt atgaagctgt ttttgaagat acacgtttga tcattatcgt
    13441 attgaatacg ttcatgctgg ctttcttatc tgcattgatc gccacgatca tcgggacatt
    13501 tggggctatg ggaatctatt acacgaaaaa acgacgtgca cggacagcgt tgttgagcat
    13561 gaataacatc ttattagtct ctcctgatgt tattatcggt gctagtttct tgattttctt
    13621 tacactacta ggattcggat taggttttca gagtgtattg ctttcccata ttgctttcag
    13681 cattccaatc gtcgtcttga tggtcttgcc aaaattgcag gaaatgaacg attcaatggt
    13741 cgatgcggct agagaccttg gtgctaataa tttccaagta gtcaaaaata tcattttgcc
    13801 ttatctttct ccaggaatca tagctggata ttttatggca tttacctatt ctcttgatga
    13861 ttttgcggtt acatttttcg tcacaggaaa tggctttacg acgctctcag tagagatcta
    13921 ttctcgtgca agacaaggaa tcagtttaga aatcaatgcg ctaagtgcgt tagttttctt
    13981 gttctccatg atattggtca ttggatacta ctttattagt aaagaaaacc gtccaaaacg
    14041 gctgaagaaa aacagaagag tacgcgaggg ggtggcacgg ctgcgatgaa aaaattacaa
    14101 tctctgtttt taggtattct catcattatt gcggtgctgc tgcttggtgt tcgtcaactt
    14161 gaaaaaacaa gtggcatgag tggagcaaag gtacttacca tctataactg gggagattat
    14221 atcgatccta gtttgatcac gaaatttgaa aaagaatatg gatacaaagt gaattatgaa
    14281 acatttgatt ctaatgaagc gatgttcaca aaaatccagc aaggtgggac aaactacgac
    14341 atcgctatac caagtgaata tatgatccaa aaaatgatca aagaaaaact agtacttcca
    14401 ttagatcatt cgaagatcaa aggattaaat aatattgatc cacgattttt agacttggat
    14461 tttgatccag ataatcagta ttcgattcca tatttttggg gaactttagg gatcgtatat
    14521 aacgataaat tctttgatgc aaatcaaatt aagcattggg atgatttgtg gaagccggaa
    14581 ttaaaagaca gtttgatgct gattgacggt gcacgagaag ttatggggct ttctttgaat
    14641 agtttaggct attctttgaa tagtaagaat atggcacagt tgacagaagc agcaaataaa
    14701 ttgaatcatt taacaccaaa tgtcaaagca atcgtggcag atgagatcaa aatgtatatg
    14761 atcaatgaag aagcctccgt ggcagtgaca ttttctggcg aagctgccga tatgatgtgg
    14821 gaaaatgaac atttacatta tgtgatccca ccagaaggat ccaatctttg gtttgataat
    14881 atcgttatgc caaaaaccgt gaaaaataaa gaaggtgcct atgatttcat caactttatg
    14941 ctagaaccgg aaaatgcggc acaaaatgca gagtatattg gttattctac accaaatgaa
    15001 gaagcaaaag cgttattgcc aaaagacatt tcgtcggacg aacaattcta tccatctgat
    15061 gacacgatct cccatcttga agtctacaaa gatctaggat cgaaatacct aggcatctat
    15121 aatgacctat tcttagaatt caagatgtat aggagataat ggcaaaactc agtaagaatg
    15181 gggacttatc aacgttctaa agagctaaaa atcgtccttt ttctatctga gttttccaat
    15241 cttctaccaa aagcggatga aacgccgctt tttttgtgtt attttatttt ttctaccacg
    15301 tttttctacc agagaaaagt ggacaaaacc attgtaaaat agggcccgag acgggctagt
    15361 aaatctaaga ttgaaaaagt tacagaagga taaagtaatt ggaaactatg caaatatact
    15421 taacccttct gtaatctatt aataaaacta atatatgaat ccagttcaat atttgtagct
    15481 atgaaaaaac ataaaaatca tattagataa aaactaaatt ggtagataaa ttattttgtc
    15541 agatatctga ggcaaatatt cgactatttg tgtttcaaag taaaaattgg aatacaaata
    15601 tagaatagac atattgcagc cgatagttta ttatgctttt agacacatta aactggagga
    15661 aaaaatgaaa aagagcgcta aagatcgagt ttttgatgag ctgatacgac taacagggaa
    15721 tatgtcttct gtcactgccg agaaaatttc gaagaatcta ggtatctctc gtcagaatgc
    15781 cagccattat ttgacgagat tagtggaaga taaaaaggtc gagaaattac cgggaaagcc
    15841 tgttttgtgg aagcctttag atgaatatgc ggttgtagat aatacggagc aaataaacga
    15901 agcctttcat tctgtagttg gtcatgacgg aagtctgaga gaggtcattc aaaaatgtat
    15961 agccgctgtc aaatatccgc ctaacggact cagtgtatta ataaatggag ccacgggtgt
    16021 tggaaaaagt tttttagcca caaaaatttt tgaatacgct atccatgagc aaattattga
    16081 aaaagatgca ccatttgcta tcttaaactg tgccgattat gcggacaatc ccgaactgct
    16141 ttcggcaaca ctgtttggat acaagaaggg ggcctttacg ggggctgaaa aggataccga
    16201 tggtttattg gcaacggcca acaacggcta tctttttctc gatgaggtcc atcgcctatc
    16261 gaaagaaaat caagaaaagc tctttctatt tattgatact ggaaactatc gtccagtcgg
    16321 tgaaaatgta aattggcatt ctgctaaggt tcgttttatt ttcgccacga cggaaaaagg
    16381 tgaaaattat ttattagaca cctttgatcg gcgtattcaa attagtgtta tgttgccaac
    16441 ttttgaagat cgccccattc gagaacgatt agagctgatt caattatttt ttcaaaatga
    16501 agcggatgtt ttacaaaaag atattattgt ttcaagagaa gccttgtctt taatgttatc
    16561 tcatcccttt agtgggaata ttggtaagct gaagaatatc attaaaatca gttgtgcgga
    16621 tgtgtatagt cgaacacaag aagaaccatt aacgattggg aaaaatgaga tcttgattca
    16681 gttaaatatg ctggaatccg aagtagaaag cttgccgatt gctagtttat tgataaagaa
    16741 aaatcaaaag ataaaaacgg atcgaatcaa accgtttgat tatgattcga ttttggaaaa
    16801 aattactcag ctgataaatg aaggtaattt caatgaaaat ttttcttcga taaagaatga
    16861 agtacagcat ttggtgcatc acattgataa ggattctttt ttatcaatga gtagaaaatt
    16921 gatgattcag atgtttagaa aggtctgggt atccgtttta ggaaaaacgt acggattgac
    16981 gacgagtgga cctgttgcag aaattattag cagatcctat gtcatttccc caagctataa
    17041 gacagactta accgggataa atcgtacatt agctgcaaat ctacctcgga cgacttactt
    17101 atgtaatcag ttcttggagc atttaccacc gctctcagaa aaggacgaga actttctaag
    17161 actgcttatt gtcgtgactt tatctgatta tgtagatgaa tccatcgagc tgaagggcct
    17221 gttgcttgct cacggagata gtacggctag cagcattcaa gcagtagtga atcaattatg
    17281 cggcaattat gtttttgaag cgttggatat gccagtagag agtaatgtgt cagagattgt
    17341 cgaatcgaca aggaatttcg tgaaacggca agtgaataca gatagtctag tgctgatcgt
    17401 agatatgggc tctttaaatc aaatttacac acagatcaag aacgatttaa aaggggagtt
    17461 acttcttctg aataatttga cgacttccat tgcacttgat attggcttga agatgacgat
    17521 gaatgcgcca tttaaacaga ttgcggagga tgcagcagac aagtattcaa tcaatgttca
    17581 atactttgaa ggcttttctc aaagtagcaa cattatcatt tcctgtatgt cgggtttggg
    17641 gatttcggac aaattgaaag atgtttttcg tcatcattta tcggatgaat ccatcgaagt
    17701 tttcacgaag gactacagag aactacgtga attaatcgac caaaatgatg aaacttattt
    17761 tgacaagaca aaattggtca tcacgacttc ggatcttcca cgttcctttt caattcctaa
    17821 tattaatgtt tatgacatct tagacggtga tggagcagcc aatttggagc atattttgag
    17881 tcctgaaatg aagccggcag aatttaaccg cttgattcag gagctagtaa aattcttttc
    17941 gttggaagga attgcggacc gtttgaactt tctgaaccca acgatcgtga tgagtgaagt
    18001 ggaaacagtc atttctaaat atgaaaatta ctatcatttc actttaaatg gccgtgtaaa
    18061 actaaatctc tatatgcata cggcattgat gatggaacgt ttattcttgg ctcggacaga
    18121 aaaatctgaa gaaccgaatt atgatttgaa accagatgaa gatgaatttt attatgtttc
    18181 tagaagtatt tttcaatcaa tggagatgaa atataatttt aaagtcaatg cttatgagct
    18241 ttcgctgctc tatgaattat taggaccgta tattcaaaaa taaagtgtct aaaaaagtgt
    18301 ctaatctgtt ttttttaggt tagacacttt ttttaatgtg aattaacgtg attaaaccaa
    18361 tgtttttaaa agttggcaca ggaattgctt atataaaagt gaacagaaag aaaggaatta
    18421 accatatgaa gaaaattcta atagcgactc acggtcactt agcttcaggc tttcttagca
    18481 gtattcaatt actgacgggg aaggttcaag agattacggt gattaatgct tatgtggatg
    18541 agtgtgattt tgaacaggag ttgaatgtat ttgtaaacca ggtgaacgag caggatcaga
    18601 tttttgtttt taccgattta tttggcggaa gtgtcaatca gaagattacc aaaacctttt
    18661 tggagaaaga gattgctgta actattttgt ctggctttaa tctgccaatt ctgctcgaaa
    18721 tagtattggc aaccaaggaa ttaacaaagg aggatattca ggaactggta aataaatgcc
    18781 aacaggaatt aaagatgacc attcttgatg aggtacatgt agtaaatgaa gaggaagatt
    18841 ttttttagga ggaagaaaaa tgattaaagc agtgcgaatt gatgaacgat taattcatgg
    18901 acaagtagcg atgacttgga caaagagtct aaatttaacc gggttagtag tagcaagcga
    18961 tgaggcggct accaacgata ttcaaaagat gacattgaag atggctgcac catcgaatgt
    19021 caaagtcatt attaaaacag ttgatgacgc gattcatttg ttaaaagatc cgcgggcgaa
    19081 ggacatgcgc ttgatggttt tagtccctaa tgtcaaagat gcggtaaaag ttgcgaaagc
    19141 tttcccaacg gaaatcgaat taatgaacgt ggggaatgct gggaaaatga caccgccatc
    19201 ggaagataag aaaaccttat caaaggaagt gatgttaacg acgtcagaaa ttcaagactt
    19261 aaaagaatta gttgaacttt atccagatac cgtttttcag cctacaccgg cgatggaaaa
    19321 acgttcagct atctctattt tgagtgattt ttagaaaaag gaaggagaaa aattatgtat
    19381 gatgcatttg tagtcgcttt ggccgttttt attggaattg ctggacatga aatgttcggg
    19441 atgtccatgc tgagtcgtcc gattgttgtt gctccgttag caggagcgtt gatgggggat
    19501 ttgcagacgg ggttgattgt cggggcgtca ctagaagcca tctttatggg tgtcgtcaat
    19561 atcggaatct ctagtacggc ggagccttct ttggctgctg ggctggctac agcgttcgca
    19621 atcaaaatgg gaggaaacgt ggatgcgatt attcccattg tctttccact agcagtatta
    19681 ggattacagt taatgaatat gattttttct tttgtatgtg gaccgatggc tgctaaattt
    19741 ttcaaatttg caaaatctgg tgatcaaaaa ggaatcatca atctgcactt ctttatgtgg
    19801 tttgtccact atgcgcttta tgctcttatt ccattctttg ccgttttatt tggagcagat
    19861 gttgtcaaaa gcgtcttgga taatattcca gaggtcatca tgaacggatt aacagtcgct
    19921 ggaaatctgc tgccggcagt tgggatggcg atgctgttac ggatgctttg gagtaaaaat
    19981 attgctgtgt ggtttttctt tggggccgtt gttatggcgt atctcgagtt accattgatt
    20041 gcaattgccg cagttggaac gattattgca attgttatag cccaaagaga tctacaacta
    20101 aaaaatatat ctgttcatgc agttcaaagc gatgctgctg taaatgcaac gcttgaccaa
    20161 gaagaggagg atttctttca atgaaattag ctgatggaat ttctaaagat gagaaaaaat
    20221 tggttcggaa gatgttttgg cgctcgatga ctatgtatgc ttcggtgaat ccggtgacga
    20281 tgggcggcgg tggtttcgcc tattctatga tgcccttcat taatcatttc tacaaaagtg
    20341 aagaagatcg agctgcggcg ctggaacgtc atacacagta tttttcgacg accattcctt
    20401 gcgcgtcctt tgtcatgggg attgcggcat cgatggagaa ggaaaattct gaaaaagctg
    20461 gttttgacgt ggaaagtatc aacgcgatca aaacgagttt gatgggacca ttggctggaa
    20521 ttggcgattc attgttctgg ggagtttggc gtgtcgttac cgcgggacta gcgatcaact
    20581 tagccaatgc cggaaatatt ttggcaccaa ttatcttttt agttatgttc aatataccta
    20641 attacttgat gcgatactat ggcggcgttt taggctattc attaggctct aaatatattg
    20701 aaaagctgta tgcgaacggg ttaattgaaa ttctgacaaa agctgctagt atcttgggtc
    20761 tgatcatggt aggtgcaatg acgatcagta tggtcaaatt caataccgtt ttgagctttt
    20821 caatgaaggg cgaagaacta atgaatgtcc aagaaatcct cgatagtatt tttaaaggaa
    20881 ttgtaccgtt attggtaaca ttgagctgtt tcaaattact agataaaaaa gtcggcgtca
    20941 tcaaaattat ttttggaatg gtcgtcttgg gaattttatt gtcattactt ggaatcgcat
    21001 agtaggaagc tgcagctact aggctgcagt ttttttagca aggagggaaa agatgactat
    21061 taatgaagta atttattcac ccaaacaccc attttttaat gctgagttaa acgacggaaa
    21121 tgtgactact tcaaacgaat acagctacta tcggaatatt cctgatgtaa agggattgac
    21181 cttcgatgta agtaatcaaa agacattagc aaaccttgat gtttgcggga cgattcaaac
    21241 gttgaccttt tatcgagaaa atttactaac tgaggaaaaa ccaggtgttt gggtcaataa
    21301 acagctctct caaaccaatt cgttatcctt aaaggttgaa gtaaacggga ttgtctacca
    21361 tttgtcagaa tcggatcatc gtattgaagt cgatgtgatt caagactgtt tgcctagata
    21421 tattcatcat tattcgacgt ttaaggtcat tgtagtatcc tttgctccca tactggctaa
    21481 taagcgttta agtatgcttg ttcaacaggt ttacattgtg aatgaaacac acgaaagcct
    21541 tcaagtttca gtgaaagatg tgccttcgta tcaagagaag tattcggatc agcaaaatgt
    21601 gttgatttct caaaaaggaa ataaggacac gatcgcacaa caggaagtgg caagcttcgc
    21661 cattgctttt gtggacccaa atgcttttga agaagtaatg acttttcagg aatctgatat
    21721 cgaaaaatgg ctgaaggaaa cattgaacta ttttgctcag ctatttgggc agctgtcaat
    21781 gccaaacgat agagttgtcc atctctatcg tcgtgcactt tatcaaagct tttctagttt
    21841 cggtatgaat cgacagggac aggtcgtcgg cagcaattgg ggcagctatc ctgctactaa
    21901 tcgaatttgg aataaggata tgtattacgc atcattgcca ttcaccatgt ttgaaccgga
    21961 gttatgtcaa aaaacggttc tatggtttga tcaatatggc gttaagtttc ctggttcaaa
    22021 atttacagga ggtatcaatc attctctgag taattcgtta tccagtgcgt tactggctag
    22081 tctttatttt gaacatactg gggatacaaa attctttgag cggtgtccgc aggttctact
    22141 gaatgcttca agaattattg aggacatatt agctcaacgt cggtcagggg aaccaatgct
    22201 ttttccatct gtttggttat cagacgcctt tgcacttgga aagtatcata ccggcagcaa
    22261 tctttgtctt tggaaggcct gtattggact tgcagaaatt tttgaagccc tagaacaact
    22321 gagtcttagc aagcgataca aaaacattgc ctgtaaacta aaggaatcaa tttgtaaaca
    22381 aatgactatc gatggtcctt ttggtgagca atttttggag ggaatcggtg atgaggagaa
    22441 aacaacctat tcagtgaagc actatcaaaa accgattctt gagcaagggt taatcttttt
    22501 aagtgatgtg attgtggatg gaaaaattaa tttattgatg catgatggag aagaatcaga
    22561 cactacgttg atgccttttt atcaattttt agataataaa gatcatttat atcagaacac
    22621 catggccttt tctgctagtt ctttcaatcc aacctatagc gaggaaatca aaggcatcac
    22681 ttggggatta gaatccggcg ccacttttcc tggctttatc actgttttaa tgagcgagct
    22741 gcacaaccct caagctttcg taacccgatt agaagaacta atttgtttgg cagatttaga
    22801 cggttcgtgg tggtggtggc cgtatcgctt agaggctcaa caaggagaag tcgtgcgaga
    22861 ctttggctgc ggcaaatgcg gctgggcatc gggtcttttt gtcagcctaa tgatcagtca
    22921 atatttcgga ttgaaattca aaaaaggagt attgcaaatt gatcctgttg ataatttaac
    22981 gttttcttgg aagaatttga agttcggtca agccttcatt tcgattgaat gtacgcaatc
    23041 cgagttgagt atttcaaatt tgtcagaaaa gccattgaaa ataagtgata cgaaaaaaat
    23101 tcttcacgta gaaaagggaa aaacgattca cattcaaagg agaaaaagat gagtatacta
    23161 aaaaatatta cccaatttaa aaaaatccag agtcgagcaa tcactcccga aaatccaaca
    23221 ggcgagaaag gtaaagctgc gatgttgcca agcgagctag gaccaagccg caaaggtcgt
    23281 ggcgcaattc cgctgacttc tggtgaagaa actgtgattg cggatattac tggaacggga
    23341 ataattcgtc atatgtggat gacgattcgt gatcgaacag aatttggcag ttttgtttta
    23401 cgtgatttaa tcttaagaat ttattgggat gggtcggata gccctgctgt tgaaagtcct
    23461 ttaggggatt tcttttgtaa tgggtttgga gagcgttgcg aggtgaactc gttgccgata
    23521 gtggttaatc ctacaggggg ctttaattcc tatttcgaaa tgcctttcca aacatccgct
    23581 aaaattacga tcgagaattt acatccgaag gatatgtcct cattttttta tacgataaat
    23641 tacgctttgg tggacgaatt ggaagaaaat agcctatatt ttcacgcaaa ttggcatcgg
    23701 gaacgcatta ccaaattaca ggaggattat aagttaattg atcgattaaa gggtcaaggc
    23761 tattacgtgg gtacttacct tgcgcttacc tgcctagaac gttattggtg gggcgaaggc
    23821 gagtttaaat tctacattga tggggatagt gagtatccga ccgtaacctc aacaggttca
    23881 gaagattatt ttggcggggc gtgggccttt catgagtacg atgagttcgg acgtccacat
    23941 gcgaaaaact attcaacagc ctttttaggg tatccttacc aatcaaatag agatgccaca
    24001 agagatcgtt ttgaaacggg aaaattgaat gccgttcatg cctttggtga cgacgcaatg
    24061 ccacgacatg gtctttatcg ttggcatctt caagacccca tctgttttac cgaagattta
    24121 tcggtcactt tacaacaaat cggcaacgat gatgttcgct tatttgagcg gcaagacgat
    24181 gttgctagtg tcgcttactg gtattcaaca gaagcttcag gaaatactaa acgtcatttc
    24241 actgatcgag aacgcttgcc gcgttaatga agtgtttttc acatattcga agggaggaac
    24301 ctatgatgat atatggggca attgaagctg gagggactaa atttgtctgt gcagtcggta
    24361 ctgaggaatt aacgattttg gaaagagtca gttttcccac aacaactcca gttgaaacaa
    24421 tgaagcaggt cattgatttt tttaaacctt ttaaggaaca attggcaggc atcggggtcg
    24481 gctgctttgg tccaattgat attcaatgca aatcaacgag tttcggtcat atcacatcaa
    24541 caccaaaact tgcttggcaa aatttcaatt ttgtaggaac gctaaagcaa tatttcaata
    24601 ttccgattga atggacaaca gatgtcaatg cggcttgcta tggtgaatat gttgctggtt
    24661 ctggcaaagg gttagcaggt gttgcatact tcactgtggg aacaggtatc ggaggtggtg
    24721 ccttgttcaa tgggactttc gtggaagggt tcagtcatcc ggaaatgggg catatgttga
    24781 tcaagcgaca tccaaaggac aatttttcag gaaactgtcc cttccaccaa gattgtttag
    24841 aggggatggc agcaggacca gcgattgaaa aacggttggg tgtaaaaggc cagaatttat
    24901 tggctgacga ttcgttttgg cagattgaag ccttttacct tgcccaatgt gcatacaata
    24961 caacactgat gttttcgcca gatagaatta tttttggcgg tggtgtaatg aaacaagaac
    25021 acatgaagaa aaaagttcag gataaatttg ttgagcttat aaatggatat gttgaaatac
    25081 caccaattga tagctacatt attacgcccg aattaggtga taatgccgga ataatcggcg
    25141 gattagcatt ggccagaaaa gcggtaagaa ataagcaacc ataattaatg aatgtttcat
    25201 tgatactcat gtcaaaagta tttaaccgag taatcaaatg atcaaatgaa gttggataaa
    25261 gcgaaaatga attcaaataa aaaggctcta agtcaaatcg tgttgttttc attaaatcac
    25321 tgaaaggaaa tgcttctgcg tttccttttt tctttttgtt gtgcgctgta taccttgagg
    25381 atattcactg gtggcgtaca cttaaatagt aatatattgt gttactttat gtctgtggta
    25441 atagttatag ttgcttacca atgtatgttc atttttcgaa atgttgactt cataatcctt
    25501 ggcttaatgt ttgaattttt ttggtgatag attttttcag ccgtataaaa taaatcgtaa
    25561 t
//
LOCUS       DASJOX010000028        25199 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJOX010000028 DASJOX010000000
VERSION     DASJOX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.028"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..84
                     /locus_tag="U4A12_001090"
     tRNA            12..84
                     /locus_tag="U4A12_001090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="U4A12_001091"
     tRNA            88..160
                     /locus_tag="U4A12_001091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="U4A12_001092"
     tRNA            168..249
                     /locus_tag="U4A12_001092"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="U4A12_001093"
     tRNA            267..339
                     /locus_tag="U4A12_001093"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="U4A12_001094"
     tRNA            351..422
                     /locus_tag="U4A12_001094"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="U4A12_001095"
     tRNA            439..524
                     /locus_tag="U4A12_001095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="U4A12_001096"
     tRNA            535..608
                     /locus_tag="U4A12_001096"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="U4A12_001097"
     tRNA            618..691
                     /locus_tag="U4A12_001097"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="U4A12_001098"
     tRNA            711..783
                     /locus_tag="U4A12_001098"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="U4A12_001099"
     tRNA            811..884
                     /locus_tag="U4A12_001099"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="U4A12_001100"
     tRNA            898..971
                     /locus_tag="U4A12_001100"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="U4A12_001101"
     tRNA            995..1084
                     /locus_tag="U4A12_001101"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="U4A12_001102"
     tRNA            1097..1170
                     /locus_tag="U4A12_001102"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="U4A12_001103"
     tRNA            1189..1264
                     /locus_tag="U4A12_001103"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="U4A12_001104"
     tRNA            1266..1336
                     /locus_tag="U4A12_001104"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="U4A12_001105"
     tRNA            1350..1423
                     /locus_tag="U4A12_001105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="U4A12_001106"
     tRNA            1448..1535
                     /locus_tag="U4A12_001106"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="U4A12_001107"
     tRNA            1557..1628
                     /locus_tag="U4A12_001107"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="U4A12_001108"
     tRNA            1651..1740
                     /locus_tag="U4A12_001108"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="U4A12_001109"
     tRNA            1753..1826
                     /locus_tag="U4A12_001109"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="U4A12_001110"
     tRNA            1845..1917
                     /locus_tag="U4A12_001110"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="U4A12_001111"
     tRNA            1921..1993
                     /locus_tag="U4A12_001111"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="U4A12_001112"
     tRNA            1998..2068
                     /locus_tag="U4A12_001112"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="U4A12_001113"
     tRNA            2081..2154
                     /locus_tag="U4A12_001113"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="U4A12_001114"
     tRNA            2166..2237
                     /locus_tag="U4A12_001114"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="U4A12_001115"
     CDS             2503..3696
                     /locus_tag="U4A12_001115"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN1773488.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="U4A12_001116"
     CDS             3826..5298
                     /locus_tag="U4A12_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1773489.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="U4A12_001117"
     CDS             5381..6169
                     /locus_tag="U4A12_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN1773490.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="U4A12_001118"
     CDS             complement(6236..6904)
                     /locus_tag="U4A12_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN1773491.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="U4A12_001119"
     CDS             7019..8731
                     /locus_tag="U4A12_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773492.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="U4A12_001120"
     CDS             8734..10503
                     /locus_tag="U4A12_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773493.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="U4A12_001121"
     CDS             complement(10708..11274)
                     /locus_tag="U4A12_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1773494.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="U4A12_001122"
     CDS             complement(11274..12230)
                     /locus_tag="U4A12_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN1773495.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="U4A12_001123"
     CDS             complement(12230..12886)
                     /locus_tag="U4A12_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1773496.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="U4A12_001124"
     CDS             13256..15274
                     /locus_tag="U4A12_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1773497.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="U4A12_001125"
     CDS             15701..18115
                     /locus_tag="U4A12_001125"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN1773498.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="U4A12_001126"
     CDS             18463..20016
                     /locus_tag="U4A12_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1773499.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="U4A12_001127"
     CDS             complement(20397..22181)
                     /locus_tag="U4A12_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773500.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="U4A12_001128"
     CDS             complement(22196..23917)
                     /locus_tag="U4A12_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773501.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="U4A12_001129"
     CDS             24017..24778
                     /locus_tag="U4A12_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1773502.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7795 a         4629 c         5364 g         7411 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catattttt
//
LOCUS       DASJOX010000029        25194 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJOX010000029 DASJOX010000000
VERSION     DASJOX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.029"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U4A12_001130"
     CDS             99..746
                     /locus_tag="U4A12_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1773503.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U4A12_001131"
     CDS             1139..3784
                     /locus_tag="U4A12_001131"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN1773504.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U4A12_001132"
     CDS             4094..5407
                     /locus_tag="U4A12_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN1773505.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U4A12_001133"
     CDS             5397..6050
                     /locus_tag="U4A12_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1773506.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="U4A12_001134"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="U4A12_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="U4A12_001135"
     CDS             6787..7872
                     /locus_tag="U4A12_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1773507.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="U4A12_001136"
     CDS             7869..9761
                     /locus_tag="U4A12_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1773508.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="U4A12_001137"
     CDS             9768..10145
                     /locus_tag="U4A12_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1773509.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="U4A12_001138"
     CDS             10299..11081
                     /locus_tag="U4A12_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEN1773510.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="U4A12_001139"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="U4A12_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEN1773511.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="U4A12_001140"
     CDS             complement(11996..12055)
                     /locus_tag="U4A12_001140"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEN1773512.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="U4A12_001141"
     CDS             12447..13109
                     /locus_tag="U4A12_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1773513.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="U4A12_001142"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="U4A12_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="U4A12_001143"
     CDS             13637..13837
                     /locus_tag="U4A12_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1773514.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="U4A12_001144"
     CDS             complement(14091..15770)
                     /locus_tag="U4A12_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1773515.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYFIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="U4A12_001145"
     CDS             complement(15772..15984)
                     /locus_tag="U4A12_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN1773516.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="U4A12_001146"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="U4A12_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN1773517.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="U4A12_001147"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="U4A12_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN1773518.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="U4A12_001148"
     CDS             19959..20156
                     /locus_tag="U4A12_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773519.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="U4A12_001149"
     CDS             20309..20794
                     /locus_tag="U4A12_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN1773520.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="U4A12_001150"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="U4A12_001150"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN1773521.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="U4A12_001151"
     CDS             21671..22198
                     /locus_tag="U4A12_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773522.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="U4A12_001152"
     CDS             22289..23026
                     /locus_tag="U4A12_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1773523.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="U4A12_001153"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="U4A12_001153"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN1773524.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="U4A12_001154"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="U4A12_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN1773525.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8391 a         4102 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga aatagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DASJOX010000030        24750 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJOX010000030 DASJOX010000000
VERSION     DASJOX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.030"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(221..1003)
                     /locus_tag="U4A12_001155"
     CDS             complement(221..1003)
                     /locus_tag="U4A12_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773526.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1068..1904)
                     /locus_tag="U4A12_001156"
     CDS             complement(1068..1904)
                     /locus_tag="U4A12_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1773527.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1922..3331)
                     /locus_tag="U4A12_001157"
     CDS             complement(1922..3331)
                     /locus_tag="U4A12_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1773528.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3345..5168)
                     /locus_tag="U4A12_001158"
     CDS             complement(3345..5168)
                     /locus_tag="U4A12_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1773529.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5448..5675)
                     /locus_tag="U4A12_001159"
     CDS             complement(5448..5675)
                     /locus_tag="U4A12_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773530.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6011..6337)
                     /locus_tag="U4A12_001160"
     CDS             complement(6011..6337)
                     /locus_tag="U4A12_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773531.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6383..6745)
                     /locus_tag="U4A12_001161"
     CDS             complement(6383..6745)
                     /locus_tag="U4A12_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEN1773532.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6782..6997)
                     /locus_tag="U4A12_001162"
     CDS             complement(6782..6997)
                     /locus_tag="U4A12_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1773533.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7124..7459
                     /locus_tag="U4A12_001163"
     CDS             7124..7459
                     /locus_tag="U4A12_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1773534.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7535..9532)
                     /locus_tag="U4A12_001164"
     CDS             complement(7535..9532)
                     /locus_tag="U4A12_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773535.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9630..9905)
                     /locus_tag="U4A12_001165"
     CDS             complement(9630..9905)
                     /locus_tag="U4A12_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773536.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10137..10871
                     /locus_tag="U4A12_001166"
     CDS             10137..10871
                     /locus_tag="U4A12_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1773537.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10910..12052
                     /locus_tag="U4A12_001167"
     CDS             10910..12052
                     /locus_tag="U4A12_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1773538.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12147..13538)
                     /gene="sufB"
                     /locus_tag="U4A12_001168"
     CDS             complement(12147..13538)
                     /gene="sufB"
                     /locus_tag="U4A12_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN1773539.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13543..14013)
                     /locus_tag="U4A12_001169"
     CDS             complement(13543..14013)
                     /locus_tag="U4A12_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN1773540.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14000..15235)
                     /locus_tag="U4A12_001170"
     CDS             complement(14000..15235)
                     /locus_tag="U4A12_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1773541.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15232..16518)
                     /gene="sufD"
                     /locus_tag="U4A12_001171"
     CDS             complement(15232..16518)
                     /gene="sufD"
                     /locus_tag="U4A12_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN1773542.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16531..17304)
                     /gene="sufC"
                     /locus_tag="U4A12_001172"
     CDS             complement(16531..17304)
                     /gene="sufC"
                     /locus_tag="U4A12_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN1773543.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17501..18349)
                     /locus_tag="U4A12_001173"
     CDS             complement(17501..18349)
                     /locus_tag="U4A12_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1773544.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18373..19059)
                     /locus_tag="U4A12_001174"
     CDS             complement(18373..19059)
                     /locus_tag="U4A12_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773545.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19052..20086)
                     /locus_tag="U4A12_001175"
     CDS             complement(19052..20086)
                     /locus_tag="U4A12_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1773546.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20149..20405)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20481..20819)
                     /locus_tag="U4A12_001176"
     CDS             complement(20481..20819)
                     /locus_tag="U4A12_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN1773547.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20821..21177)
                     /locus_tag="U4A12_001177"
     CDS             complement(20821..21177)
                     /locus_tag="U4A12_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN1773548.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21183..22370)
                     /locus_tag="U4A12_001178"
     CDS             complement(21183..22370)
                     /locus_tag="U4A12_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1773549.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22561..23406)
                     /locus_tag="U4A12_001179"
     CDS             complement(22561..23406)
                     /locus_tag="U4A12_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1773550.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23640..24605
                     /locus_tag="U4A12_001180"
     CDS             23640..24605
                     /locus_tag="U4A12_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN1773551.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7214 a         5216 c         4217 g         8103 t
ORIGIN      
        1 aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa
       61 tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa
      121 aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac
      181 aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa
      241 atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc
      301 cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga
      361 tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat
      421 cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg
      481 tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg
      541 tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg
      601 tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag
      661 tgtgcatagt taaaattccc acgcttttac taataaattc actataacca gttctttcaa
      721 tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca
      781 ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga
      841 atacagcttt actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc
      901 caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag
      961 aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag
     1021 cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt
     1081 gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct
     1141 tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact
     1201 gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata
     1261 aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt
     1321 tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg
     1381 gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca
     1441 gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag
     1501 ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa
     1561 acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat
     1621 agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg
     1681 caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca
     1741 tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct
     1801 ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct
     1861 aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc
     1921 tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta
     1981 ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct
     2041 tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa
     2101 catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa
     2161 ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat
     2221 tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta
     2281 gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg
     2341 cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat
     2401 ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa
     2461 aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc
     2521 cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta
     2581 agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg
     2641 aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca
     2701 actgatgata caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa
     2761 agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa
     2821 ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag
     2881 taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc
     2941 cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc
     3001 cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt
     3061 tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc
     3121 ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt
     3181 caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat
     3241 cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct
     3301 ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc
     3361 aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg
     3421 ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg
     3481 gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag
     3541 ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct
     3601 ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca
     3661 ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta
     3721 gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca
     3781 acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca
     3841 attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga
     3901 acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca
     3961 aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc
     4021 atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta
     4081 attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta
     4141 gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct
     4201 ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt
     4261 gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt
     4321 aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac
     4381 gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc
     4441 ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag
     4501 gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat
     4561 accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga
     4621 tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct
     4681 gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa
     4741 atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg
     4801 gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg
     4861 acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt
     4921 gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc
     4981 acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct
     5041 ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt
     5101 aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg
     5161 taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat
     5221 caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac
     5281 gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa
     5341 ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg
     5401 gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa
     5461 accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat
     5521 aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta
     5581 aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg
     5641 gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga
     5701 atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta
     5761 atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc
     5821 ataataattt tttctttttc atttctttta tattgttata acagtgaata acataaaaat
     5881 tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac
     5941 tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa
     6001 tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt
     6061 ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct aatatagctt
     6121 tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat
     6181 ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc
     6241 cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta
     6301 taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa
     6361 taatttgctc ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg
     6421 ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata
     6481 agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta
     6541 ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt
     6601 gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg
     6661 tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt
     6721 ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta
     6781 gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt acacttaata
     6841 tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac
     6901 ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt
     6961 cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta
     7021 tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca
     7081 aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata
     7141 tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga
     7201 aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa
     7261 cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga
     7321 caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga
     7381 agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa
     7441 tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga
     7501 gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc
     7561 tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta
     7621 tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta
     7681 aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag
     7741 ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt
     7801 tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct
     7861 ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta
     7921 aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag
     7981 cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat
     8041 gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat
     8101 taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct
     8161 cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt
     8221 tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat
     8281 aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag
     8341 tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac
     8401 gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa
     8461 gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt
     8521 ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca
     8581 tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg
     8641 aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac
     8701 atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat
     8761 aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca
     8821 tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat
     8881 actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa
     8941 gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc
     9001 ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt
     9061 tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa
     9121 agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg
     9181 cagaattatg aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat
     9241 ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg acatccagaa
     9301 taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg
     9361 gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat
     9421 agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta
     9481 ataatttttc ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt
     9541 cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat
     9601 tactatatgt tttagtctta tttgtttact caacctttat accgttccat gaaactatca
     9661 atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga
     9721 gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag
     9781 tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta
     9841 attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga
     9901 gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta
     9961 ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta
    10021 ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa
    10081 atttatttgc aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga
    10141 taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag
    10201 ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc
    10261 aattaattga caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag
    10321 agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca
    10381 atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta ctagatggta
    10441 taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact
    10501 ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg caagaattta
    10561 ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa
    10621 aaatagaaga attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc
    10681 tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat
    10741 tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc
    10801 tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg
    10861 ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat
    10921 atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac
    10981 cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga
    11041 agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc
    11101 acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt
    11161 taaagcttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt
    11221 tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg
    11281 gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta
    11341 cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa
    11401 aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga
    11461 tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac
    11521 aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa
    11581 agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc
    11641 tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc tttctatcct
    11701 caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc
    11761 taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa acactgttaa
    11821 tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt
    11881 tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca
    11941 acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa
    12001 tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg
    12061 gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat
    12121 caagggttct agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc
    12181 aatcgattga gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag
    12241 cccatgacga tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag
    12301 agttgttctt ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg
    12361 tggatctcat tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca
    12421 cattcgatat gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag
    12481 tttacttctc cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt
    12541 gcattgtgga tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg
    12601 gagagcatgg tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt
    12661 gtttttgcac ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct
    12721 cgtttggtta ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa
    12781 tacgcatctt ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa
    12841 gtaggtgccg tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc
    12901 gtacgttcga attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca
    12961 tcgactttga cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc
    13021 agcgctgcta atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat
    13081 tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat
    13141 tcatctttca tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga
    13201 taagctcgtt cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt
    13261 acgtcttccc agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg
    13321 aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct
    13381 agagatttca gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca
    13441 cggatcactt cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga
    13501 aacccgaatt tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg
    13561 gtaatgatgg aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc
    13621 aagtgtcgca catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc
    13681 gcctaattgg tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt
    13741 taaatgttct gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact
    13801 tgccgtactg atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt
    13861 tacatcgact tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct
    13921 tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg
    13981 acggtataaa ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga
    14041 ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag
    14101 cagtcgctga tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga
    14161 cggctacccc ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat
    14221 tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat
    14281 caattgcttg taattttggc aacacataag cggctagttc ttcttcatat cgatggatat
    14341 tgtccatacc gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga
    14401 tatttggtgt ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt
    14461 cgacaaaatc gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc
    14521 gtttaccata taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa
    14581 agaaatcaca gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat
    14641 ctactacgat gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga
    14701 agacacctaa gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct
    14761 ttttcgctgc atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga
    14821 cagcacctgt tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt
    14881 aagagagcac tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc
    14941 aattcaagct tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga
    15001 tgaacgcccg tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg
    15061 tatggacacc gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga
    15121 gtacttgttt tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat
    15181 tgacgtcttg aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt
    15241 caacttccct tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt
    15301 taagacagaa ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc
    15361 tctactcatc agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc
    15421 agcagttact tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc
    15481 agaaagcatc aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat
    15541 gtggccgata ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat
    15601 atgcccgatc gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct
    15661 gcccgagctg attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga
    15721 gtcaaagtca gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc
    15781 atctcgtaaa atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc
    15841 tacggctgaa tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc
    15901 tgatgatttt gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact
    15961 atgttcatct gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg
    16021 aatgaataag gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat
    16081 accgctgttc ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc
    16141 ttttttcata taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc
    16201 ggtaaaaatg actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga
    16261 accttgttgg acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt
    16321 ttcactgatg gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg
    16381 atcgatatgc ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct
    16441 catccaatca ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag
    16501 ccagtttttt tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc
    16561 taattcttta ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt
    16621 ccctgtcatg actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt
    16681 caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt
    16741 tactccttta gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa
    16801 aattgcaaat gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc
    16861 gccagaaaaa ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc
    16921 cattttttca tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg
    16981 tttcgcattg atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg
    17041 atattgcata gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac
    17101 atttttcccg tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat
    17161 agcggcagat aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc
    17221 ggttttcatt gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag
    17281 attttttatt tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca
    17341 ttttagacta attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta
    17401 tttattatta aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa
    17461 gccattacag acttatttct catctttttt gttccatacc ttattttgca tcagccttta
    17521 ctggtttaac agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa
    17581 gcacttcaat cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa
    17641 tattcacgta aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc
    17701 ctgcgccgta agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg
    17761 tagaagctaa aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt
    17821 caatatcatc gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag
    17881 catcttgtag aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg
    17941 ttgcaccatc ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt
    18001 cgatgtggac agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca
    18061 aataaggaat atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg
    18121 gtaatacata gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg
    18181 gtttcacatg ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg
    18241 tgtcgtctgc tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc
    18301 ctacagtcag caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc
    18361 ccctatatcc tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac
    18421 gataaatacg attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg
    18481 ctggtagcct tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat
    18541 cgcagtaaat ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat
    18601 actttcaggt atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc
    18661 tgcttccagt acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa
    18721 tggtgctgcg gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac
    18781 gattgcttta gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa
    18841 aatattactg atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg
    18901 tccaatagag taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga
    18961 gattaatgtc atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg
    19021 tttaaaatct agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta
    19081 ctccttgttc tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca
    19141 agacagtcaa tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt
    19201 tgacatcgat gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt
    19261 cgctaaaggt caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata
    19321 gttctgtcga atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt
    19381 ttttcggttg tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca
    19441 tcacagcgac tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga
    19501 tggtcaaatt caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt
    19561 gcgggtccaa tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg
    19621 ctcgtgcgat accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc
    19681 cgcgtccttc taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct
    19741 ttggtacgcc agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata
    19801 acaggttgaa atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt
    19861 tttttaattt tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa
    19921 gtccattcag gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat
    19981 aaatatcccc tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt
    20041 tcccattgaa tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt
    20101 tctcttatga tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa
    20161 agcttgaact tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc
    20221 agtacttgga cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta
    20281 agtttgctta cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc
    20341 attttcagca aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct
    20401 ttctcgtttc atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt
    20461 tttcaagtga ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa
    20521 cttaagatcc acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt
    20581 ttatcattaa cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt
    20641 tccgcttcaa cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga
    20701 ttggcaaacg tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat
    20761 tcttttccat taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt
    20821 ttatatcagt tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc
    20881 tcgtaagatc agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt
    20941 tgaagccgta aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct
    21001 tgtgttgaac aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga
    21061 ggccttcggt acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa
    21121 ccacgctttg gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt
    21181 tcttacctcc ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac
    21241 ccgataccga tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg
    21301 atccctgtta aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc
    21361 agcatcatga tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac
    21421 acccgaatca tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata
    21481 aagccgaagt tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag
    21541 atattgctta cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct
    21601 tgggccggct gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata
    21661 cgtgcaaact gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc
    21721 gtaaccaaga agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg
    21781 atgcgccaag agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc
    21841 atcgttccaa agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag
    21901 agcttcccaa tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt
    21961 ttagttacga tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg
    22021 aaagtaaacg caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga
    22081 tcatagaatt tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc
    22141 agtttccaga tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact
    22201 aaataccaga caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg
    22261 ctaagtgcta catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc
    22321 acgccataat caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc
    22381 ttttcttaaa aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt
    22441 tccatagaca tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt
    22501 acaaaagcct actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat
    22561 ctaccagctg agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat
    22621 ctcttcggca atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa
    22681 atatcgatgg aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa
    22741 aatcgagcga ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat
    22801 catcttctta tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc
    22861 agagtttgct tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg
    22921 gtcttccatc aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc
    22981 aaaatcggat tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat
    23041 ctcaaaatgc actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata
    23101 gacagcaaag gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag
    23161 caatttttgc tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa
    23221 gtcggcttgc ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt
    23281 caattcccgc tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac
    23341 ttctgctgct ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt
    23401 aaacatggca ccacttccat aactttttat gataattact ataaaaacta tttgatttaa
    23461 atatacaaaa aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt
    23521 aacttagatg aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt
    23581 acaatgtatt cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata
    23641 tgaagaactt tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt
    23701 cccttggaaa tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta
    23761 tctctatgat tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga
    23821 aacaaacgtt gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca
    23881 tggttatttg tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt
    23941 ggctgtgcgc tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta
    24001 tcaaagctga tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat
    24061 ttggggtcat cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt
    24121 ggatttcatt gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg
    24181 taagaaatat ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc
    24241 tttgcctaac aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat
    24301 tgttcgcttt atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca
    24361 agcttcgttt ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag
    24421 ctatatttac tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt
    24481 tcttaaaagt tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt
    24541 acgtacgttt cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg
    24601 cttaataatc gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc
    24661 caaacaaaaa cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc
    24721 ttttgaacac cgtttaccaa gaggtcgtaa
//
LOCUS       DASJOX010000031        24652 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJOX010000031 DASJOX010000000
VERSION     DASJOX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.031"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..787
                     /locus_tag="U4A12_001181"
     CDS             59..787
                     /locus_tag="U4A12_001181"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN1773552.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            787..1041
                     /gene="purS"
                     /locus_tag="U4A12_001182"
     CDS             787..1041
                     /gene="purS"
                     /locus_tag="U4A12_001182"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN1773553.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1043..1714
                     /gene="purQ"
                     /locus_tag="U4A12_001183"
     CDS             1043..1714
                     /gene="purQ"
                     /locus_tag="U4A12_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN1773554.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1715..3937
                     /gene="purL"
                     /locus_tag="U4A12_001184"
     CDS             1715..3937
                     /gene="purL"
                     /locus_tag="U4A12_001184"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN1773555.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3922..5361
                     /locus_tag="U4A12_001185"
     CDS             3922..5361
                     /locus_tag="U4A12_001185"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN1773556.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5384..6436
                     /locus_tag="U4A12_001186"
     CDS             5384..6436
                     /locus_tag="U4A12_001186"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN1773557.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6433..7011
                     /gene="purN"
                     /locus_tag="U4A12_001187"
     CDS             6433..7011
                     /gene="purN"
                     /locus_tag="U4A12_001187"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN1773558.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7008..8543
                     /gene="purH"
                     /locus_tag="U4A12_001188"
     CDS             7008..8543
                     /gene="purH"
                     /locus_tag="U4A12_001188"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN1773559.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8559..9833
                     /gene="purD"
                     /locus_tag="U4A12_001189"
     CDS             8559..9833
                     /gene="purD"
                     /locus_tag="U4A12_001189"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN1773560.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9955..10869
                     /locus_tag="U4A12_001190"
     CDS             9955..10869
                     /locus_tag="U4A12_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1773561.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11002..12567)
                     /locus_tag="U4A12_001191"
     CDS             complement(11002..12567)
                     /locus_tag="U4A12_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1773562.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12560..14011)
                     /locus_tag="U4A12_001192"
     CDS             complement(12560..14011)
                     /locus_tag="U4A12_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1773563.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14025..15041)
                     /locus_tag="U4A12_001193"
     CDS             complement(14025..15041)
                     /locus_tag="U4A12_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1773564.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15261..16349
                     /locus_tag="U4A12_001194"
     CDS             15261..16349
                     /locus_tag="U4A12_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN1773565.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16403..17929
                     /locus_tag="U4A12_001195"
     CDS             16403..17929
                     /locus_tag="U4A12_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN1773566.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17931..19136
                     /locus_tag="U4A12_001196"
     CDS             17931..19136
                     /locus_tag="U4A12_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773567.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19393..20370
                     /gene="guaC"
                     /locus_tag="U4A12_001197"
     CDS             19393..20370
                     /gene="guaC"
                     /locus_tag="U4A12_001197"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1773568.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20634..22058
                     /locus_tag="U4A12_001198"
     CDS             20634..22058
                     /locus_tag="U4A12_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1773569.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22519..23628
                     /gene="pdhA"
                     /locus_tag="U4A12_001199"
     CDS             22519..23628
                     /gene="pdhA"
                     /locus_tag="U4A12_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN1773570.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23631..24608
                     /locus_tag="U4A12_001200"
     CDS             23631..24608
                     /locus_tag="U4A12_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN1773571.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         7978 a         4202 c         5575 g         6897 t
ORIGIN      
        1 aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat
       61 ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga
      121 gaacttgatt tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga
      181 ccagataagt ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct
      241 gactcaaaga ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc
      301 aaaaaaagta caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt
      361 ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta
      421 ctataaggat gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca
      481 acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat
      541 tttgaccgaa ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg
      601 ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg
      661 ggatgaacgt acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt
      721 gatcccagtc tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg
      781 agtatgatgt atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa
      841 ggggaggcag taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga
      901 ataggaaaat actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt
      961 gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc
     1021 aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg
     1081 tgacgcagat atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg
     1141 gcatgatgag tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta
     1201 tggtgactat ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat
     1261 tcgttttgct gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac
     1321 agaagcagga cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa
     1381 aactgtacca ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga
     1441 aataatctat cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact
     1501 tcaagaacta aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag
     1561 cgtggaaaac attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca
     1621 tccagaacgt gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc
     1681 cattttaaaa aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc
     1741 tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg
     1801 aatgatcgaa gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc
     1861 tgttatgtgg agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc
     1921 tactagtgga cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg
     1981 tgatggacta gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc
     2041 ttatgaagga gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc
     2101 tcgtccaatt gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa
     2161 atatttacta gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc
     2221 gactgtaggc ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc
     2281 aatgtgcgta ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg
     2341 taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt
     2401 tgcctcagaa gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga
     2461 tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga
     2521 ttctctaata ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat
     2581 ggcttcaaaa gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac
     2641 tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt
     2701 taaatcagga gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt
     2761 cactatcggt aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt
     2821 agcaaatctg ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa
     2881 agaaccagct cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga
     2941 acctggacaa gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat
     3001 ctacgaaact tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc
     3061 tgctgtgatg agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc
     3121 gcgctatctg tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag
     3181 aaatattatt gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc
     3241 tcctgataaa ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc
     3301 gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga
     3361 tggtcaagca atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa
     3421 acatatcact actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac
     3481 gtttgcagat tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt
     3541 gatccgaaac tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat
     3601 ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact
     3661 ggcagaatca gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc
     3721 tcaattattt gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac
     3781 acgattcgaa gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc
     3841 gataatcgaa atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa
     3901 gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg
     3961 atcttcggtg tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt
     4021 cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag
     4081 gggcatcgag atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag
     4141 ttaacaggag aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg
     4201 gacaatattc agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat
     4261 ggtaacttga tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc
     4321 cattctaact ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat
     4381 tttttaaata aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta
     4441 atgacagaaa cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc
     4501 ggacaaatga aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt
     4561 ggggcacgtt ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga
     4621 atccagattg attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc
     4681 tattttgcca gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat
     4741 atgggacgga atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct
     4801 aattcttctt tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc
     4861 ggattagtaa aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga
     4921 gagcaaggtg ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg
     4981 attatggtcg atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg
     5041 aaagaagcag gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca
     5101 tgtttttacg ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt
     5161 gatgaaatca aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg
     5221 atcgaatcga tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc
     5281 aacggagatt atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa
     5341 gaaaaagtat cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa
     5401 tgcatacgca aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa
     5461 gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg
     5521 ttgttttgat ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg
     5581 cgtggggacg aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga
     5641 ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt
     5701 agactactta gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt
     5761 agcagatgga tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc
     5821 tggtatgtac tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa
     5881 atctcaatta ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc
     5941 tagcgggatt cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca
     6001 gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga
     6061 gccaacgaaa atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg
     6121 agctgcacat atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc
     6181 tatggctgca caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca
     6241 aaaatatggt gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat
     6301 ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg
     6361 cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat
     6421 aggagaagaa cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg
     6481 ctagccgatt atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat
     6541 cagcctgaag catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt
     6601 tcacctaaag aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa
     6661 gaaaaaaaga tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg
     6721 ctgaaaaatt atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct
     6781 ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt
     6841 cattatatcg accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt
     6901 gaacaggagg acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc
     6961 tatccagaag tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat
     7021 tgatcagtgt atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag
     7081 gagtagaaat catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa
     7141 cgctttcgat cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac
     7201 ttcatcctaa aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg
     7261 caatggaaac ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca
     7321 aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg
     7381 gtggtcctag tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg
     7441 atccatctga ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt
     7501 ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga
     7561 tcgctcaata cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt
     7621 atgaacgaaa acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc
     7681 agtccgctct tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac
     7741 tttcttataa taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg
     7801 agccaacggt agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga
     7861 ttttagaagc gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca
     7921 tcgttttgaa tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag
     7981 aaatcatcat tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa
     8041 acatccgatt actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg
     8101 tctcagtctt aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag
     8161 agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg
     8221 cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga
     8281 cagtaggaat cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac
     8341 aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg
     8401 attcggttga atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca
     8461 tcaaagacca agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg
     8521 atgttcggca ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg
     8581 caggtggtcg tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg
     8641 tgtattgtgc gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca
     8701 gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta
     8761 tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat
     8821 tgaaaatctt cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta
     8881 aacaattaat ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg
     8941 aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct
     9001 tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag
     9061 aacaaatgtt gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt
     9121 ttttggcagg agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga
     9181 tggtcatttc gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg
     9241 gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg
     9301 aagaagtttt aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg
     9361 ttttatatgc gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc
     9421 gtttcggtga tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag
     9481 taatcgatga tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca
     9541 atgtaggcgt tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc
     9601 taccagattt tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg
     9661 gcaaactagt tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca
     9721 aagaagcaca gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt
     9781 atcgtacaga tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct
     9841 gcttccattt ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt
     9901 ttcataaaga gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa
     9961 aaaattcaat taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt
    10021 atcaacaatg cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat
    10081 ttttttgacc atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct
    10141 ctagcaaaaa catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt
    10201 ccaggtgtga tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg
    10261 acccgtctga aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta
    10321 gaaccagagg aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat
    10381 tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa
    10441 ccattagtag ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg
    10501 attcatgtca atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat
    10561 tcccgattgc atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa
    10621 ggtgtattta tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca
    10681 gctaaatatc aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc
    10741 aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct
    10801 tcagaaatta atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt
    10861 ttaccttaaa gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac
    10921 ttgaaagctg ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta
    10981 tcattgtacg gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata
    11041 ttcttttgga gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt
    11101 ataaccaatt tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt
    11161 tgctaattcg attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga
    11221 aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga
    11281 catatctgga tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg
    11341 atatttgttc cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac
    11401 tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca
    11461 atgaaccatt tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt
    11521 caagacaaat aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg
    11581 ttcgatgtac ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa
    11641 gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat
    11701 ttgttcttta agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg
    11761 taattcgctg attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc
    11821 cttttctaat tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc
    11881 ttcaagagaa tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac
    11941 ttgttgattc tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa
    12001 gtctgtactg acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg
    12061 attgaaagat tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc
    12121 gttcaagaaa tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt
    12181 ttgctgcaaa tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt
    12241 aatcggttta agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa
    12301 atcgtcgaat ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt
    12361 tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg
    12421 taagtcgata attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat
    12481 tggataataa gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc
    12541 gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag
    12601 ggatcatcag tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt
    12661 gatcatttcc gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac
    12721 tgaaatctga atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag
    12781 ggatgccaat cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg
    12841 taatacgaat aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa
    12901 tcaatggctg caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga
    12961 tcttatagtc aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat
    13021 ggctaatttc tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa
    13081 agccggaaag tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag
    13141 aaataatggc atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac
    13201 gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta
    13261 gatcttccgc cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca
    13321 gggttaaacg aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat
    13381 tgatcggttc ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa
    13441 tggtactaag aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg
    13501 tatttttttt gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca
    13561 gcagttgagt gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt
    13621 ttgccaagat attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta
    13681 gcgatacatc taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa
    13741 tatcattcct aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc
    13801 cagaaatcaa gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat
    13861 ctgctttttg gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca
    13921 gtatgctcag tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa
    13981 aaatctgttt tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt
    14041 gggatgttag ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt
    14101 aatgtttttc ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag
    14161 ccgacatcag gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc
    14221 atatcttctt gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt
    14281 tcttttatcg cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg
    14341 atttcggata aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga
    14401 ataaaatcac cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca
    14461 tcttttttta gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg
    14521 gtcggtgtgg agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa
    14581 tttgtgaagt gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg
    14641 ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg
    14701 acagggatcc ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa
    14761 ggagtaaaaa tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc
    14821 cgttcctgat tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca
    14881 gtgataattg attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa
    14941 ccaacgacta gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa
    15001 gcgattacaa caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg
    15061 atttgttagt actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga
    15121 aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa
    15181 gtaaatccca taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca
    15241 attgaaatgg gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta
    15301 ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta
    15361 cggggtatgt aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga
    15421 acaacatggt ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag
    15481 ataaagtaga aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac
    15541 ttgtgattgc ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag
    15601 cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt
    15661 acgcgacttt tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact
    15721 taggattgaa agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg
    15781 acaacaacgc actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca
    15841 acggtcagtt ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct
    15901 gggatggttc tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc
    15961 aaacattgga tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat
    16021 taaaaggcgt ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg
    16081 cgacagttgc tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag
    16141 acacacgtat cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag
    16201 aggttccggt caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt
    16261 tagcaaatac agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt
    16321 attataaaga atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta
    16381 agtcagagag aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa
    16441 aaaattttct ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat
    16501 ccatgcattg tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg
    16561 cttgtatcca tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt
    16621 taaagatttg aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact
    16681 aagtccttat ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg
    16741 cgtgattgat tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt
    16801 gtctatcgac ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga
    16861 aatagctaaa gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc
    16921 attgaatgaa gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg
    16981 tgtaacctcc atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat
    17041 caccatttta cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga
    17101 gcatattatt cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc
    17161 ggatattgga gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca
    17221 cgagcgaatc ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat
    17281 agctggactg atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta
    17341 tggaagcaag atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt
    17401 tccttcagcc attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact
    17461 gaatctgctg atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca
    17521 aaaaggagta ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat
    17581 gcgtataaaa acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca
    17641 aaaagtcgtt ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc
    17701 aacccgtggg atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc
    17761 agcaaatggc aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg
    17821 tgatcgaatc tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc
    17881 agatcaggaa gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg
    17941 caaaaaatct tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc
    18001 tttttggtcg ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga
    18061 tgacggatgg tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc
    18121 atatattagt actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt
    18181 cggtagggtc agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc
    18241 aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat
    18301 ggcaaggttt ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat
    18361 tgctgatgtt tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc
    18421 cagctgtttt ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc
    18481 ttcacttctt gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat
    18541 ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct
    18601 tggtaaaggc ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt
    18661 atcaagggta tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt
    18721 tgacaaataa aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg
    18781 ctgaactatc agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag
    18841 tcatggcagc tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag
    18901 caggtacatc gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt
    18961 ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga
    19021 acaatggcat gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga
    19081 tcttgctttt agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga
    19141 acgccggtta ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta
    19201 aaagcgaaca ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat
    19261 tcgtataaat tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg
    19321 cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag
    19381 gaggataatg taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt
    19441 atcgtaaata gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa
    19501 atgccagtag tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta
    19561 gcggaaaatg gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt
    19621 atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag
    19681 gaatattcct ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc
    19741 gacatcgcgc atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat
    19801 ttgcctgaaa cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg
    19861 gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca
    19921 aaaatcaaga caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg
    19981 aaagcagctc gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca
    20041 aaatctgtcc gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag
    20101 gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct
    20161 gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa
    20221 cacaaaggat ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt
    20281 tcttatgctg gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa
    20341 aattcgattt ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa
    20401 aaagttgtcc tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt
    20461 gtttggctgg aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg
    20521 actgtgataa gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc
    20581 tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg
    20641 aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag
    20701 aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc
    20761 aactttttgg tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg
    20821 gggtactaac gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat
    20881 tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact
    20941 ggtatgagca aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat
    21001 acgctagcga gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg
    21061 aagaaatctt agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca
    21121 aaatcaaacg gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg
    21181 tgactggtgg cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag
    21241 aggggatagc tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag
    21301 gaacgcgttt atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag
    21361 aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta
    21421 caggaaaagt gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc
    21481 ggcttccaat tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag
    21541 tccttcctat acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg
    21601 ttttaggaaa aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac
    21661 tagctcaagc gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg
    21721 ctaatgtatt taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag
    21781 aaaaaagccg actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa
    21841 ttttaggtgg ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag
    21901 cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata
    21961 taaatggaat cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt
    22021 caaagatgaa aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa
    22081 agaatataac agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac
    22141 gatataaaga aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa
    22201 ataaatgttt gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt
    22261 aagtttgatt agaaaataga aaaaatgcag atgaaaaata aaacgctttc cttattgtga
    22321 ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt
    22381 tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa
    22441 tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga
    22501 gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta
    22561 gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt
    22621 aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg
    22681 gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc
    22741 ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat
    22801 aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta
    22861 cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa
    22921 gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt
    22981 gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat
    23041 ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca
    23101 aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa
    23161 actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc
    23221 gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc
    23281 agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta
    23341 tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa
    23401 gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa
    23461 gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac
    23521 aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa
    23581 tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa
    23641 aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg
    23701 tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat
    23761 tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg
    23821 caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct
    23881 ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc
    23941 gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc
    24001 atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc
    24061 gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa
    24121 gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag
    24181 tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag
    24241 atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc
    24301 ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg
    24361 tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac
    24421 aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt
    24481 cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag
    24541 cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg
    24601 aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa tt
//
LOCUS       DASJOX010000032        23830 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJOX010000032 DASJOX010000000
VERSION     DASJOX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.032"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..789)
                     /locus_tag="U4A12_001201"
     CDS             complement(55..789)
                     /locus_tag="U4A12_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1773572.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="U4A12_001202"
     CDS             1116..1526
                     /locus_tag="U4A12_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN1773573.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="U4A12_001203"
     CDS             1519..2220
                     /locus_tag="U4A12_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN1773574.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="U4A12_001204"
     CDS             2220..3806
                     /locus_tag="U4A12_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773575.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="U4A12_001205"
     CDS             3825..4046
                     /locus_tag="U4A12_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN1773576.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="U4A12_001206"
     CDS             4043..4804
                     /locus_tag="U4A12_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN1773577.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="U4A12_001207"
     CDS             5184..5693
                     /locus_tag="U4A12_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1773578.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="U4A12_001208"
     CDS             complement(5768..6307)
                     /locus_tag="U4A12_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN1773579.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="U4A12_001209"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="U4A12_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN1773580.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="U4A12_001210"
     CDS             7320..8171
                     /locus_tag="U4A12_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1773581.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="U4A12_001211"
     CDS             8199..8834
                     /locus_tag="U4A12_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN1773582.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="U4A12_001212"
     CDS             8854..9627
                     /locus_tag="U4A12_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1773583.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="U4A12_001213"
     CDS             10035..10832
                     /locus_tag="U4A12_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN1773584.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="U4A12_001214"
     CDS             10810..11223
                     /locus_tag="U4A12_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1773585.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="U4A12_001215"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="U4A12_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1773586.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="U4A12_001216"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="U4A12_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN1773587.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="U4A12_001217"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="U4A12_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN1773588.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="U4A12_001218"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="U4A12_001218"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN1773589.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="U4A12_001219"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="U4A12_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN1773590.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="U4A12_001220"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="U4A12_001220"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN1773591.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="U4A12_001221"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="U4A12_001221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN1773592.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="U4A12_001222"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="U4A12_001222"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN1773593.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="U4A12_001223"
     CDS             21907..22485
                     /locus_tag="U4A12_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1773594.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="U4A12_001224"
     CDS             22537..23019
                     /locus_tag="U4A12_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN1773595.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="U4A12_001225"
     CDS             23136..23510
                     /locus_tag="U4A12_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773596.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6648 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DASJOX010000033        23200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJOX010000033 DASJOX010000000
VERSION     DASJOX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.033"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="U4A12_001226"
     CDS             363..731
                     /locus_tag="U4A12_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN1773597.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="U4A12_001227"
     CDS             911..1852
                     /locus_tag="U4A12_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN1773598.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="U4A12_001228"
     CDS             1921..2652
                     /locus_tag="U4A12_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN1773599.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="U4A12_001229"
     CDS             2796..4976
                     /locus_tag="U4A12_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN1773600.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="U4A12_001230"
     CDS             4996..5478
                     /locus_tag="U4A12_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN1773601.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="U4A12_001231"
     CDS             5494..6255
                     /locus_tag="U4A12_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1773602.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="U4A12_001232"
     CDS             6386..7720
                     /locus_tag="U4A12_001232"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN1773603.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="U4A12_001233"
     CDS             8027..8512
                     /locus_tag="U4A12_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN1773604.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="U4A12_001234"
     CDS             8505..9188
                     /locus_tag="U4A12_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN1773605.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="U4A12_001235"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="U4A12_001235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN1773606.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="U4A12_001236"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="U4A12_001236"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN1773607.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="U4A12_001237"
     CDS             12804..13433
                     /locus_tag="U4A12_001237"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN1773608.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="U4A12_001238"
     CDS             13485..13817
                     /locus_tag="U4A12_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1773609.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="U4A12_001239"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="U4A12_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN1773610.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="U4A12_001240"
     CDS             14643..16016
                     /locus_tag="U4A12_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1773611.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="U4A12_001241"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="U4A12_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN1773612.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="U4A12_001242"
     CDS             17071..17511
                     /locus_tag="U4A12_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1773613.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="U4A12_001243"
     CDS             17655..18356
                     /locus_tag="U4A12_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN1773614.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="U4A12_001244"
     CDS             18353..19474
                     /locus_tag="U4A12_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN1773615.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="U4A12_001245"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="U4A12_001245"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN1773616.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="U4A12_001246"
     CDS             20853..21761
                     /locus_tag="U4A12_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN1773617.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="U4A12_001247"
     CDS             21796..22068
                     /locus_tag="U4A12_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN1773618.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="U4A12_001248"
     CDS             22079..23125
                     /locus_tag="U4A12_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN1773619.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7814 a         4039 c         5085 g         6262 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg
//
LOCUS       DASJOX010000034        23064 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJOX010000034 DASJOX010000000
VERSION     DASJOX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.034"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(110..565)
                     /locus_tag="U4A12_001249"
     CDS             complement(110..565)
                     /locus_tag="U4A12_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773620.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="U4A12_001250"
     CDS             complement(719..1927)
                     /locus_tag="U4A12_001250"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN1773621.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="U4A12_001251"
     CDS             complement(2039..2371)
                     /locus_tag="U4A12_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1773622.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="U4A12_001252"
     CDS             2519..3394
                     /locus_tag="U4A12_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1773623.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="U4A12_001253"
     CDS             complement(3476..4057)
                     /locus_tag="U4A12_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN1773624.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="U4A12_001254"
     CDS             complement(4063..5322)
                     /locus_tag="U4A12_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1773625.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="U4A12_001255"
     CDS             complement(5324..6370)
                     /locus_tag="U4A12_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773626.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="U4A12_001256"
     CDS             complement(6605..6880)
                     /locus_tag="U4A12_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN1773627.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="U4A12_001257"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="U4A12_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN1773628.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="U4A12_001258"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="U4A12_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN1773629.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="U4A12_001259"
     CDS             complement(9979..10659)
                     /locus_tag="U4A12_001259"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN1773630.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="U4A12_001260"
     CDS             complement(10708..11343)
                     /locus_tag="U4A12_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1773631.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="U4A12_001261"
     CDS             complement(11409..12812)
                     /locus_tag="U4A12_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN1773632.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="U4A12_001262"
     CDS             complement(12799..13830)
                     /locus_tag="U4A12_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773633.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="U4A12_001263"
     CDS             13861..14082
                     /locus_tag="U4A12_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN1773634.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="U4A12_001264"
     CDS             complement(14247..14984)
                     /locus_tag="U4A12_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN1773635.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="U4A12_001265"
     CDS             complement(15137..16477)
                     /locus_tag="U4A12_001265"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN1773636.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="U4A12_001266"
     CDS             complement(16714..18237)
                     /locus_tag="U4A12_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN1773637.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="U4A12_001267"
     CDS             complement(18615..19226)
                     /locus_tag="U4A12_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1773638.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="U4A12_001268"
     CDS             complement(19556..20272)
                     /locus_tag="U4A12_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1773639.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="U4A12_001269"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="U4A12_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN1773640.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="U4A12_001270"
     CDS             complement(20831..21628)
                     /locus_tag="U4A12_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN1773641.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="U4A12_001271"
     CDS             complement(21598..21969)
                     /locus_tag="U4A12_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1773642.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="U4A12_001272"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="U4A12_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN1773643.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DASJOX010000035        22770 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJOX010000035 DASJOX010000000
VERSION     DASJOX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.035"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(67..1329)
                     /locus_tag="U4A12_001273"
     CDS             complement(67..1329)
                     /locus_tag="U4A12_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773644.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1322..2221)
                     /locus_tag="U4A12_001274"
     CDS             complement(1322..2221)
                     /locus_tag="U4A12_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773645.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2366..4144)
                     /gene="uvrC"
                     /locus_tag="U4A12_001275"
     CDS             complement(2366..4144)
                     /gene="uvrC"
                     /locus_tag="U4A12_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN1773646.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4326..4640)
                     /gene="trxA"
                     /locus_tag="U4A12_001276"
     CDS             complement(4326..4640)
                     /gene="trxA"
                     /locus_tag="U4A12_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN1773647.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4727..7087)
                     /locus_tag="U4A12_001277"
     CDS             complement(4727..7087)
                     /locus_tag="U4A12_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1773648.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7202..7749)
                     /locus_tag="U4A12_001278"
                     /pseudo
     CDS             complement(7202..7749)
                     /locus_tag="U4A12_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7807..8244)
                     /gene="zapA"
                     /locus_tag="U4A12_001279"
     CDS             complement(7807..8244)
                     /gene="zapA"
                     /locus_tag="U4A12_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN1773649.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8446..9363
                     /locus_tag="U4A12_001280"
     CDS             8446..9363
                     /locus_tag="U4A12_001280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN1773650.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9619..10530)
                     /locus_tag="U4A12_001281"
     CDS             complement(9619..10530)
                     /locus_tag="U4A12_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1773651.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10933..11511)
                     /locus_tag="U4A12_001282"
     CDS             complement(10933..11511)
                     /locus_tag="U4A12_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1773652.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11531..12199)
                     /locus_tag="U4A12_001283"
     CDS             complement(11531..12199)
                     /locus_tag="U4A12_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773653.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12214..13284)
                     /locus_tag="U4A12_001284"
     CDS             complement(12214..13284)
                     /locus_tag="U4A12_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773654.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13455..14819)
                     /locus_tag="U4A12_001285"
     CDS             complement(13455..14819)
                     /locus_tag="U4A12_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1773655.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14816..16375)
                     /locus_tag="U4A12_001286"
     CDS             complement(14816..16375)
                     /locus_tag="U4A12_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773656.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16431..18911)
                     /locus_tag="U4A12_001287"
     CDS             complement(16431..18911)
                     /locus_tag="U4A12_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773657.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18895..19230)
                     /locus_tag="U4A12_001288"
     CDS             complement(18895..19230)
                     /locus_tag="U4A12_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773658.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19244..20278)
                     /locus_tag="U4A12_001289"
     CDS             complement(19244..20278)
                     /locus_tag="U4A12_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1773659.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20368..21048)
                     /locus_tag="U4A12_001290"
     CDS             complement(20368..21048)
                     /locus_tag="U4A12_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1773660.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21332..22435)
                     /locus_tag="U4A12_001291"
     CDS             complement(21332..22435)
                     /locus_tag="U4A12_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1773661.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         6503 a         4784 c         3870 g         7613 t
ORIGIN      
        1 taggtctata caaaaaagaa gctgggaaca ataaatgtcc agcttccttc tatatttttc
       61 tatattttat gctttttttc gttcatttct cataattgaa atcgattgtt tcaatgaact
      121 ccataatccg ccttcgctat acacgagtac attcgatttg tacatgtttg cagacaagat
      181 catcaataag aatgttacgg ctacaagtac caacaaggaa atcaatgctc cacccatacc
      241 gactgtctca tttgctaagc gaaccggcat aatatatgaa gataagaatg gaatatacga
      301 agtgatccga ataacgatat tgtttggatc tgaagctcct agtatcaatc caagcatata
      361 tccgcctaga gatagataag tcactggcaa aattgctttt gctgtatctt ctgctttgtt
      421 gaccaaagag ccacataaag ctgctaaaac agaaaaaatc aaaatgccaa agatcatgaa
      481 tagaagagaa taccacaaga atggtccaat caagttatcc aatgagatac cgtctaatat
      541 atttttgacc atttccatat ctttgaattg actgtatcct ataccaaaaa taataccgta
      601 taatgccatt tgcgttaatg caacaagtaa aacacctgtt agttttccgt aaaaatgctt
      661 ttgtgcagtc gtactggaca ggatcacttc catgatccgc gtgccttttt cagaagcgat
      721 ctcttgtgca atgatctgcg catacgtcaa aataataatg aacaagacaa tcgttgctac
      781 ataacttacg acatattgga ctgtcgagtt atcttctcca actgtcattt ttccttttga
      841 atcaaaattg actttttgtc gagtcaaact tgcttgttgg ctcaagctag ctacttcttc
      901 tggtgaaatg cctaattgac ttgctcgaag catcgattgc aagccagtca gttgttgttg
      961 tatcagtagt tgtgtacttt gtcctaatga attttcgcta taaagcatcc catttatctt
     1021 atcattctca gttgagacta ctaaatacgc atcgattttt tcttccgaaa gctctttttg
     1081 tgctttttct tcagaatcaa ctgctttgaa ctcaaaatca tctgttttga cttggctcat
     1141 ttgctgactg atttgctcat tatcagccaa tacgccgatt ttattgacat cactattttg
     1201 ttgagcaaag tttcctgcta cataaatgat acccagcatg ataaaaggga ctaatatcat
     1261 gatcaagaaa gaaatcgact taacattttt taaatagaca tctttggtaa taatccaaaa
     1321 cttacgcatc gatttcacct gctttcaatt tgaaaatctc ttccaaagtt ggcggttgct
     1381 ggttaaacat aggaatgtag ccgaactgag tcgcacgagt aaagatttct ttcccagctt
     1441 caggatcttc taaggtaatc tctaacgtat tgtcttcatg aaggatcact tttttcacgc
     1501 cttcgatttc ttctacttcc tgttggctta aaccagattc caagaataac ttcgtacggc
     1561 caaatgattc acgaatctca tgtacttttc cattcaaaac catttttccg tttcgcaaca
     1621 tgatcaaatg atcgcagatt ttttctacat tatccatatt gtggctagaa aaaatgacac
     1681 atgaaccatg ttttttcaat tccaatattc catcttttag caattcagca tttaccggat
     1741 ctaacccgct gaatggctca tccaaaatga tcaatttagg ttcatggatt aacgtggcga
     1801 tcagctggac tttttgctga tttcctttag acaaagattt tactttatcc gtctttttcc
     1861 cttttacctg aaatttttcc atccaaaaat cgatttttgg ttcaatctct tttcttgatt
     1921 tccctctaag ttctgcaaaa taaagcagtt ggtcttgaat cgttactttc ggataaagtc
     1981 cccgttcctc cggaagataa ccgatgacat cataatcttt ctccccaaga tgatggccat
     2041 tccataacac ttctccttgg tcttgtgtca aaaaatccaa gatcaaacga aaagtcgttg
     2101 tcttcccagc cccgttttgg ccaattagcc ccatgatttt tccatcgggg atttggaaag
     2161 agacatgatc gacagctgtg tagttcccaa aagttttaac aagatccttg acttctaaca
     2221 tttcttcact ccttcttcaa gaaatcacat atatccttag acaccagacg taagcgattt
     2281 attatttacc aacaaaacgt aaacgctctt tttgttgtac ataaaatgat aggacacttt
     2341 actttctttg taaagtgccc tttcctcatt cctgatgtaa tttatcaaat acgttttgtg
     2401 cgacattttt cggcagtccg atttcttgta attcttctgt cgtggctgct gtgatatttt
     2461 tcaacgattt gaactcttta agcagcattt tttttcgttt tggtcctaac ctttcaattt
     2521 catctaatcg ggaagcaaaa ctgtttttac tgcgtagctg ccgatggaaa gtgattgcga
     2581 atcgatggac ttcatcttgg attcgctgga gtaagaaaaa ctctggtgaa tttcgctcaa
     2641 gcggtactac agccaaatct ataccgaata gcaactcatt tgttttatgt ttatcgtttt
     2701 ttgccatgcc agcaatagga atatcgattc ctaattgatt ttctaagaca tcttttgcca
     2761 catctacttg tccttttcct ccatcgatca agatcaaatc aggtaaaggc aacccttcgc
     2821 gaatcactcg tgaatatctg cgatagatga cttctctcat tgatgcataa tcatccggtc
     2881 cgttgactgt ttttatttta tatttccgat attcttttct tgccggtttt ccatcaatga
     2941 atacgaccat cgcagccaca ggattcgtcc ccatgatatt agagttatca aaagactcga
     3001 tacgtattgg ggtcgggata ttcatcgcgt ttcctaaacg ttcaactgct ccgatcgtcc
     3061 gttcctgttt tctggcaatc agatcaaacc gttcattcaa agcaactgag gcattcttat
     3121 tcgccaattc aactaatttt ttcttttctc ctcgttttgg ttgcagcact ttcgtagcta
     3181 acaaagcttc tacactgggc ttgtcgatcg tatcaggaat caagacttct ttaggaatga
     3241 aatgctcatt ttcctgataa aactgaccga ggtaagtcaa gaaatcttct tctgcttcgt
     3301 tatagaaagg aaacattgaa acatctcgtt caattagctt tccttgtctt acgaaaaaca
     3361 cttggacaca catccatcct ttgtcaatcg cataaccaaa tacatctcga tccagtaaat
     3421 cagtatttgt cattttttgg cgagtcatga ttgtctcaat ggcacggatc tggtctcggt
     3481 attctgcagc tttttcaaac tccatgtttt cagcagcttt ttccattttc tgatggatct
     3541 ctgactcgat tttttcgtgt cctccgttta aaaacttttt cacttcatca acgattttcg
     3601 tatagacttt cggatctacg tcgaaataat aaggacacag acattgtccc aaatgatagt
     3661 aaagacaagg ctccttcgtc tgactaggtt tgcattttct cagaggaaat aatcgatcga
     3721 ggatcttctt tgtctcattc gccgcaccga catcgggata tggtccaaaa tacagtgctt
     3781 tgtcttttag tactttacga gtaatcacca aacggggata tttttcattc gttatcttaa
     3841 ggaaaggata ggttttatca tcctttaaca taatgttgta tttaggatca tttttcttta
     3901 tcagattgat ctctaagagc aatgcttcga tattcgattc tgtaacgatg tattcaaagt
     3961 cttcgatttc actaacaagc cgttccgttt tcgtattatg gctgccagta aaatatgagc
     4021 ggacacgatt ttttaatatt ttggctttgc cgacataaat gatcgttcca tttttatctt
     4081 tcatcaagta acagccgggc tgatcaggaa gtaaagctaa tttgtttttg attcgttcgt
     4141 tcatctggta ctccccttca tattcaatta aagttattat agcatgtctg tcaaaaaaac
     4201 aatccagcct gacaacgtaa ttatactcaa aaaatattaa tttgaataac cttaactcaa
     4261 ccgcaaaaaa accgaggacg gaactggatt cagtcccacc ctcagcttat ttttgctgta
     4321 agtttttata aatattgatc gatcatttga cgtagttgtt cttttgaatg aacaccaaca
     4381 gctttttcta ctacttgacc atcttttttc aacattaatg ttggaatgct catgataccg
     4441 aattgttgag gagtttctgg attttcatcc acatccattt tagcaattct gaattcttct
     4501 tcatcatatt cttgttccaa ctgatccaaa atcggtgctt gcatacgaca tggaccacac
     4561 caagttgccc aaaaatcaat caatacaagt cctttatctg tttcttcatt aaatgtctta
     4621 tctgtaatta cttgtgacat atcaaagacc tccattttta aaatctatga ttagtatagc
     4681 atttctatac ttggatagcg aaccatctgc ttacctttag taagctttac ttaaatttaa
     4741 caattgtcgc accatttccg ccttggtttg ccggtgcaaa ctcaaagctt ttgacgctcc
     4801 gatggttttt aagataatca gtaattccct tgcgaagtgc gcctgttcct tttccgtgga
     4861 caatagtcac ttgaggataa ccagctagaa tggcagaatc caaatattga tctacctcac
     4921 ttagcgcttc ttcgtatcgt tttcctcgaa ggtccaattg attcggcacg tgactactag
     4981 attcagatcg tacagcatgg ataactcgtt gtgtcggctc tttttgagga gcaacaggcg
     5041 tcagctcgtc ttcggacaca ttcattttta atataccgat ttctacttgc cactgattcc
     5101 cgtttttacg taaaagcgtc cctctttggc cgtaagtcgt aaccaacact tcgtctcccg
     5161 gtttcaacgt tttttgttct ttcgccttct tcaatacttt atttttcttc aaagtttctt
     5221 cttgatgtag atttgctaat tgggtctttg catcgatcaa ctgatgctct ttcacatttc
     5281 cttgcccaat ctgctgttgc attttacgaa tatcagcaat gattttttct gctttttctt
     5341 ccgcctctga cacgacttcg tttgcttttt tctttgcttt ttccatttct ttttcacgtt
     5401 cttcaaagaa atagctgtaa gcttctttca aatcatcgtg aagttcttgt gcctcagaaa
     5461 caaaatgacg catttccaga tattcggtct ctgccatttt ccggcgattc tctaaatctg
     5521 tgatcatttc gtttaaatct tggctctcat cattcatcaa ctgcttcgct tcatcaataa
     5581 cttctgtatc aagacctaat cggctagaga tttcaaacgc attgcttcgt ccaggaacgc
     5641 caatcagcaa acgatacgtt ggacttagtg tatccacatc gaactccatg ctggcattaa
     5701 tcgtatttgc ccgattgtat ccataaactt tcaattcagg ataatgcgtt gttgccatca
     5761 cataagcaga ctttttacca agatcatcta agattgcgat tgctagagca gcaccttctt
     5821 gcggatctgt tcctgcaccg agctcgtcaa ataataccag acttttttca ttgacttttg
     5881 ataaaatatc tacagtgttc gtcatatgag aagaaaaggt cgatagactt tgctcaatcg
     5941 actgttcgtc tcctatatcc gcaaatactt cctcaaaaat accgatttga ctttcttcac
     6001 ctgcaggaat cggtaatcct gcttgcccca tcagttgtaa cagacctaaa gtcttcaacg
     6061 tgatcgtttt cccacctgtg ttaggtcctg taatgacgat ggcttgatag tcttttccga
     6121 ttgtgatgtc attagaaact actttttcct gatcgatcag tggatgtctt gcttgtttca
     6181 agacgacatg attgtcttca ctgattcccg gcacgatggc ttttacttct ttaccaaatc
     6241 gtgcttttgc attcatcaga tccattttac caatcacata agcattgtgg atgatttctt
     6301 gacgatgggg aacaagttca gcagaaagtt cacttaaaat acgttgaatc tcatttcgtt
     6361 cagcgatttg gtattgacgc aaacgattat tcaaatccac cacttgtttt ggttcaataa
     6421 ataaagtctg tcctgaagca ctttgatcgt ggactacacc gccaaataca ccgcggtatt
     6481 cttgtttgac tgggataacg tagcggtcat ttcgcatggt gatgatcgca tcacttaaat
     6541 actttgcatt tttcccacgg acgatcccat ctaactgttc acgaacagct tgttcacttc
     6601 gccgaatgtt ctgtcttatg ctctttaact caggagaagc gtcatcagtc acgcgtccgt
     6661 cttcatcgat tgcttccttt aaacggcggc tcaaaacagg gatcgtgact aactgatctg
     6721 cccacatata caaacgtgca aattcgattt cactgtcaga aagatcatca atgaaacgat
     6781 tcaattcgct tgtcgtagac aatactctgg aaacttgtgc aagctccact ccattcaagt
     6841 ctgctccgat ctcgatccgt ttcatatggg gacggatatt ttcaagttta ggtataggga
     6901 tcccgcctcg cagacgttgc actttcaatc catcctcagt ttcttgaagc cagtttgtga
     6961 tcgtttgttt ctcactcact ggtaccaatt ctgccagttc ttcttttcct tgtgcagtca
     7021 ctacaaattg tctgaccatc tgtttgactt tttcaaattc caatgtttca aggattcggt
     7081 tattcatttt atcaccttat ctttcactat gaaaaaagag ctgtgccgga catacgctac
     7141 gcagccttct taaataaact actctgtaca aaaaacgaaa tattttccag cactattttc
     7201 cttaattcaa aatattcgtt acccaaagct catacatctt gttggtaaga aatggcgttc
     7261 tctcaacgat aaaacgagcg aaactatgct cgccgaattg attttggacc acactaatgg
     7321 aacaaaggca agtaactgca aaatcaaaaa tatagtaacg taagccatga ttagtgacag
     7381 gatgcctcct gcaatccagt ctacctgtct caagatcggc acatacgtca atccatggac
     7441 aaaaaccccc acaaatcggg taagcagcca gccaattagc aaaataatca gaaaagcagt
     7501 tcctgcataa aaagcttcat ctaggtgaaa agcaaatacc tgatcaaaaa atgctaactt
     7561 tgatgttggg gtgacagctg ggtatggaat gtataattcc aaatgacttg ctaatggttt
     7621 gtaaaaatgc tgggcagcaa taaatgacaa tacatatcca atcgcgaata tgatttgcaa
     7681 agcaaacccg cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata aaatcagcaa
     7741 actcagcatg ctacgttcct ttctcttcta acttttttaa atttttctta ttttgataaa
     7801 aggatcttaa ttagaagatt tttgtttaat ttgtcctttt cgtttctcgt ttaaaatctg
     7861 ttgtgcttcc acatgatttt taatggtatg atctgcttga ccattttctt tcaatacttc
     7921 ccgagcttcg ttctcgattg cttcaatgcg tttcacacga ttttcaagct ctgtaaattt
     7981 gatcattttt ttctttaatt ctgctgtttc ttcttccagc tccaagattc tttcttgttt
     8041 cttcaattga tccgaaagcg cattgacagc catcaagatt gctgcttgtt cattatccat
     8101 ttgtggtgat aattgtttca attctgctaa ctgttcattg atcagcttcg ttacgatatc
     8161 catatgatgc ttggtttccc gtccgataat agtatatgtt tggttggcaa tcactgcttt
     8221 gtagcgtgtt ttttcgtgcg ccatacgaat catttcctcc tatttgatga aagttttatt
     8281 tagtgtacca tgaagttggt tctttcaccg ttctccacaa atatttttca catacttaac
     8341 tattatatga taaactttaa aaaaatgcta tgtctcgcaa tctcttagaa aacgaaaagt
     8401 cgtgagttgc ggtatagttt tatgtataaa aacgaggaga attttatgag caacgtcatt
     8461 atcaaagcaa caaacgacca acttagtcaa atgaaagcat actatcgctc ttatttgtta
     8521 aataaacaga ttccttatac tagctttgct gcaaaaaaag acgggacgac cattaccgct
     8581 tatacatccg gaaaagtgat gtttcaaggt acgaatgcag caatagaagc tgataaatgg
     8641 ggggaggcac aggataaaca agccaagcca gcaaaaacag cctcttcttt gccaaaaaat
     8701 atcagtcaat tatccgtttt aggtagtgat gaggtcggaa atggcagcta ttttggtccg
     8761 ctaacagtat gtgctgctta tgtgaaacgt gatcagcttg cagaattacg taaacttggt
     8821 gttagagatt caaaagagtt aaaagatagc caaatcatcc agcttgctga catcttaaaa
     8881 aaaaccatcc cctataaact gcttgtctta tctccagaaa aatacaatga gatccaacca
     8941 aaatacaatg ccgttcggat gaaagtcgct ttgcacaatc aagcgatcca tttgctgcta
     9001 aaaaaaatcg agccagaaaa accagaagcg attttgatcg atcagtttac gccggaagca
     9061 aattttaaaa aatatgcgcg tttagaaaaa aatcaattac aagaaaaact gtacttcgtt
     9121 actaaaggag aacaatatca tttagctgtt gctgctgctt ctatcatcag tcgtgccagc
     9181 tttttggaag agttacacaa agcatcagct gaagctggaa tcacccttcc atcaggagct
     9241 ggaacgaaat ctgaccaagt agcggcaaaa ctattggaga aaggcggcat gcctatgctt
     9301 gaaaaatatg ccaaacttca ttttgccaat acagaaaaag ctttgaagct gctaaaaaaa
     9361 taatggaact gtgacaaaag tcatgtcaca gtcccttgtt ccgaataaac ggtgttctca
     9421 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
     9481 ttgctccttg tgacgcggag ttaaacggca ggctcacaac ctcttcctta aacggtgttc
     9541 aaaagcttgc atctgcgata ataagcccgg ccaaacgaaa aatatgagga gctatttttg
     9601 tttggctggg cttatttatt actgaaaata agtggtcaat gcttgtgtca aacttgcagc
     9661 aatctgctgc tgataactgt ctgttactaa ttcctttaaa tcactatcat tattcatata
     9721 acccatctcg attaaaatag aaggttgaag attttctcga agaacttgat agttcccagt
     9781 ggagattcct cttgaatcca gaggcagatc agtgaaggag tcagcaattg tctgtgctaa
     9841 tgtttcctct tgtccatagt aataatatgt cgtgattcca gttgcttcat tcgcatattc
     9901 cgtagagtct gcgtgtaaac tgataaagac atctgccttt ttttcttcac tgtagtcgca
     9961 tatatctcca agctgtacta attcatctgt agtatgggtc aaataaaccg ttgctccagc
    10021 atcctctagc gcctttttta cttttatggc tgtacttaat gttatatctt tttcttctgt
    10081 ttgttcatca ttgcttaatg ccccagtatc atttccgcca tgcccaggat ctaatacaat
    10141 cactgcatca gatagctctt ccggttttgt ttgtatattc tttgtgactt tggttccatc
    10201 ttcgagggct aaatatccag tatagccatc atttgtcttg atctgataag cattttttgt
    10261 ttcagaaaga aactccgcca gctctccgcc gtttatagtt gcaagtgggg tagttagcga
    10321 gtcatctgta tatagtgtac ggtcattttt tccgattttg attaatgtct cttgttctgt
    10381 atcatcaaat gattcctttg tctttgtaac cgtactgttc gtactgctga ccgtttgtcc
    10441 aactatcttt ttttgaccaa aaacatctat ttgaaaacag ataataatga taatgattgc
    10501 aaatacaaaa gctccaatta tttttttcat tcttgactct cacctcgtag atccagacta
    10561 agggtcaaaa cttaaaatca gcttaagtac gcaaaaaagg ggctgtaaaa gatataaccg
    10621 tagaaaataa cattactgct aaaatgattt ttttcatttt tggcatatcc tgaaatctcc
    10681 ttttgactat tcgtggtgat tcatctaccc aataaatagt ttccttcatt ctacaaacaa
    10741 ttaatttctt cgttattaaa ttaaaacact tcctggaaca ccgacataat aaataaaaat
    10801 ataccctgat aatcatgaga gaataacaaa tttgtattta tgaaaaacta cctatataaa
    10861 aaaaacaaga tataacgatc ttcgtcatat cttgcttctt cacctaacta tttttctccc
    10921 tatataacag ctttacgctt ctcctaatac accattcaaa tataaatcga tcaattgcgc
    10981 cacatttagc ggttcttcca caggtctttt cgaccgagtg atgtaaggtg ctaacactaa
    11041 gaaaaaatgc cttgctgcga tttcttttga aatcccattt cttagcacag attggtttgc
    11101 tgaaaagatt tcttcaaaag gttcacgata atttttcttc catagaaaga aaagatgatc
    11161 tcttgtttcc tttgaaagat tgctttcaag atcgtgaagc atcagaaaca aatcgaccag
    11221 atgtgtctca atcaaaaact gcgccatttt tgtcaaggtt acttgaaatc ccgtttcgct
    11281 tgtttgaacg atttctctca aagcttttcc aacatctttt aagtggtttt ctaaaactgc
    11341 cgtatatagt ttttctttat caccgaaata atgatagaga ttgggttggg tgatcccaac
    11401 tttttgtgcg atctctcttg ttgaagtgcc gagatatccc ttggacataa acaagtcagt
    11461 cgcttctttc aaaatttttt gctgtgtatc tttgttgtat cgatggacca tttttccacc
    11521 tgctttttgg ttattctttt tctttcaata caccatcttc catcataaat attctatcgc
    11581 atttgtcaat cagccgtgta tcgtgagtaa ccatgatcgt tgctttattt ttgtcttttg
    11641 attcttttgc caagatatcg actacttctc gtgccttttc tgaatccaaa ctagcagttg
    11701 gttcatctgc tagaataatg gacggatcat tgtacaatgc acgagcgatt gcgacccttt
    11761 gccgttctcc accagatagt tcttctggat acttgttgat caacttatcc acgccaagct
    11821 gctcaaataa ttcttttgct tctttttgac gatgttcatg tttgatctta tctactaata
    11881 ataactgctt ttttactgtc agaaaaggaa ccagattgga agcttgcaaa ataaagccaa
    11941 tttctttaaa acgtagttta acgcgttcac tttctttttg attactgaat tcttgatcat
    12001 tgataaatac agagccactg ctaggcgtct gcaatccgcc tgccaaagtc aaaaaagtac
    12061 ttttacctga accagaagga ccgatgattg caacaaattc ccctttatcg atactcatgg
    12121 ttgtttcttt caatgcttta actacttggt cgccatcttg gaaacttttt tctacttctt
    12181 taaattctat tgctttcatg ctcttcctcc ttcttatcca attgccttca acggatcgat
    12241 ttttacgatt gtccgaacag aaaatagtgc gcctaacaca gctactacca gcatcaacaa
    12301 gctaatgacg ccaaaaaaca gccaattgct ttggaatgga accgcatcag gtagaacaag
    12361 ggatgttccc actgttccta atagaccaag aagaatacca acaaatgata aaatgaatgt
    12421 ttgtgccact acagaacgcg caataaatcc tcctgtgatc ccttgtgctt tcattacacc
    12481 aaagatatta atcttttgca tcgtcagtac ataaatgaaa atcccgatca caatagccgc
    12541 aataacgatc aaaaatccga tcataaatcc aaaagtcaat acttgcgcat tatatccagg
    12601 taattcattg atgaatttag aaattttgat ctgttctaaa tcttcaggta aattactgat
    12661 tttacctctg acaactattg cgttaatccg tgcattttca cttgtatctt ctttttcgaa
    12721 gcggatctct tggtaagcat tgatggttgt atagagaact ggtgagacat tgaatttggc
    12781 atgatccgta aagccagtta gtttaaacgt cttgtcacta ccagataact taactgtatc
    12841 tcctacggct agaccatatt cttctttcaa actaatatcg cctactgcct catcatcgtt
    12901 atcaaaagct tttccttcaa caagattggg catgatgaat tgatttttat cgataccgaa
    12961 gaagctaaca ttgatttttc cttcttctgt actgtcttta ctagtcacga ctccaggtgt
    13021 ctgggcaaga tacgccactt tcccaccttc gacttcttct gctatcttct tagtaatcat
    13081 ggacatccct agatttgaat ttgcatcttt cgaaagcaca atcgaatctg cctcccactt
    13141 gtcgactgct gttcgattat cttgagctaa gccataagct aacccagtca aaaaaaatac
    13201 caggtaggcg atcaaaaaca tcacccctgc aactaacgca taacgtaact ttgaatgcat
    13261 aatttcattt aatgctaaaa acatttaact cctcctcctg aaataatcat tttatacaac
    13321 aattttatca cttgataaat ttttggcgaa tattatgctc aaaaaattct tccgctattt
    13381 cattatatga attttttatt ctcgattgat taatatttat tatatcccca cacaataaaa
    13441 gaccgtgata tttctcacag tcttttatct actctattgc tatttgctct cttagctagt
    13501 actttaaaag ctaactcatt gaggtagtga aaacgagagg tgtgttcaga cagaaatcca
    13561 aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg
    13621 ttcaaatctg ataataagaa ttcttggctt atcagctgtc cgccaattat ttctaatgta
    13681 tccggatggt agatcagctt tgcatttact gttttgtttt cttcatcgta ttgtggaaat
    13741 agatcaaatt ctcctgaaca cgaaccggta ttttgttcca agaatggcgc ttcatcttct
    13801 gtcagcccta cagttcctaa aaagtagtct ataatgactg ctgctgttat cttttgtctg
    13861 aggtcgtaag cggttggctg accactcaaa gttagcgcta ctgctcttgc catatgtaca
    13921 gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt tgtcaaaact gactgggacg
    13981 atatctccaa tcgcatagat gtccggatca cttgtctgca gatattcatt gactatcaca
    14041 gtctcatcct catacaaagc cactttttct tttaacaatg tcgtattagg acgagtttgg
    14101 gcagaaaaaa tgaccatatc tgcctttatt gcttgagata gaagttgtac ggatactact
    14161 ttctcttctg attttttcca atcgatcaag aattcatttg gataaaaatt gatttgctta
    14221 gtttgcagct gattttgaaa actgttgatc atatccttat cgaaatatct tgataaaaca
    14281 ttcggcatat aatcaactag atgaataatg tatcctttat ttctcaaagc atctgctaat
    14341 tccaatccaa tataaccagc accgaagatc actacttctt ttgcttcttt taaagcggca
    14401 agtacttgct tagattgcac tagattttta taggtgtaga cattttcagc ttcaatttgt
    14461 ttatacaagg tgacaggaga ggaacctgtt gctacgatta atttatcata ggttatggtc
    14521 tcgttttttc cttctttgga tgcgaggatg agctgatgtt tgtctggatc aagttcagtt
    14581 actctccatt cggtgatcag ttttgcccca ctttcttgaa gagtccgcat gttatgggca
    14641 acttcagcta aactacgtat ctttcctttt aaataaaggt tgattccatt tgaaacatag
    14701 cctagtccat ctttatgatc ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt
    14761 cttagattta gggcagctgt gatgcctgca tgggaagcac caatgatcac gattttcatg
    14821 ttttattcat ccttaaattc aatttttcta aataacattc ttttaaaaat tggttcaaat
    14881 aggggaaatc ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
    14941 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
    15001 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
    15061 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
    15121 tgatcagcgt ccgcaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
    15181 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
    15241 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
    15301 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
    15361 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
    15421 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
    15481 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
    15541 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
    15601 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
    15661 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
    15721 atttcaatcg tccttcttct gtaatagtaa aactaaactc tttttgcagc agatctaaaa
    15781 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataagga cgtccaaacc
    15841 aaaataattg gaaataaaaa taacaaattt gactttctgg accggtcagc cgcccgcttt
    15901 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
    15961 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
    16021 acagataaag aagtatctga tattttgtag aatagcgcaa gtagcaatga taataaacat
    16081 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
    16141 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
    16201 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aatctctcct ttttcctcta
    16261 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
    16321 ccaagtccac tcctccaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
    16381 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
    16441 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
    16501 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
    16561 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgt
    16621 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
    16681 ttaaataaag aagtcaatgt ccctgtcagt tgccaaggag acgtgcttgg aagccgagta
    16741 tcccgcacat caaaagaaaa atcgcctttt gcttcataat aacttgtctt caacggtttg
    16801 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
    16861 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
    16921 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaca
    16981 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
    17041 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
    17101 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
    17161 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
    17221 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
    17281 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
    17341 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
    17401 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
    17461 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
    17521 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
    17581 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
    17641 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
    17701 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
    17761 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
    17821 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
    17881 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
    17941 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
    18001 gttgaatcac ttgttgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
    18061 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
    18121 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
    18181 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
    18241 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
    18301 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
    18361 tctgccaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
    18421 gctgataata aactaccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
    18481 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
    18541 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
    18601 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
    18661 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
    18721 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
    18781 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
    18841 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
    18901 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
    18961 ccataattct ataccaatga tcaaataaag aaaacggatc ttttcttcat tcgtctccgg
    19021 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct gttttgatgc
    19081 gccattgact gaaccacttg gatgcggaac tggacttaga tcataaaatg taatcgtacc
    19141 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
    19201 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
    19261 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
    19321 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
    19381 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
    19441 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
    19501 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat tcattagacg
    19561 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
    19621 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
    19681 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
    19741 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
    19801 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
    19861 ttgctgggat tttcacttct ataggtatag ttgtttctgc atgtgctgat acattgattt
    19921 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
    19981 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
    20041 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
    20101 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
    20161 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
    20221 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
    20281 tccaccacat cctttcaaca acaactgcgg taaaaaatcc ttcacctata gtattttcac
    20341 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
    20401 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
    20461 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
    20521 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
    20581 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
    20641 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
    20701 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
    20761 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcaggaac
    20821 tacatccaat gttaaagaac ctgtctggcc agttgctgga ttttccggat ctgctggctc
    20881 tactggtttt gaaggatcag tagggttaag aggctcaacg gtatttgttg gttcagtaaa
    20941 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
    21001 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
    21061 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
    21121 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
    21181 acatgaatat tcttcctttt ttattaagta tgaaaaaata tttcaatttt attttttcgt
    21241 ttcaaattat tctattagtg acatccttga actataaaaa taggagtacg gcatcatctg
    21301 ccgcactcct atttttttcg tcatttattt cttaaataaa acgattctca cgcaatttta
    21361 tttttttgat atcttctact gtttgtgttc cagctaattg catgaccatt tccaattctg
    21421 ttttaaagaa atcaaacact tgatggactc ctgtacttcc tcctaaagac aatccataaa
    21481 taacgggtct gccaatagca acaagatcgg cacctgatgc gattgctttg aaaacatgtt
    21541 gtccacgtcg tacaccgctg tcaaacacga ttggtacacg tttatctact gcttctgcca
    21601 catattgcaa cgaatcaaac gcagcaggcc ccccatctaa ttgacggcca ccatggttag
    21661 atacccaaat tccaccagca ccagattcta atgatcgata gacatcttct tctgattgaa
    21721 cacctttcac gtagactggc aaatcagaat gagcagcgat aaattctaca tcttttggac
    21781 taagtttttg tttggaagat ttatatacag catccatcgt ttggcctact cctgattgat
    21841 aagcctgaac gatcggcatt ggcagcggga aggtaaaccc gtttctgcgg tctgtttcac
    21901 ggtttccgcc aactgttgca tccgctgtca atacgattgc ttttgctcca ttacgcttgg
    21961 ccacttcaag aatatccaaa ttgattccat tatctttgct catataaaat tgaaaccatt
    22021 gcggtgcttt ctccccgcct gcttctctta tctcttctaa agtacaagaa gcgtaagaac
    22081 ttgctgtata gatcgtccca aaatctgcta caccttttgc agaagctttc tccgccttca
    22141 catgagctaa tccatgagca gcaactggtg ccatgatgat tggtgcagtc agcatttctt
    22201 cgtcaaaatg tgtagttgta tcgggtaatt ctacatctct taatacatga ggaataatca
    22261 aacgatgatt gaatgcccgt tcattttctt gataagtaaa taagtcgcca gcaccactgc
    22321 tgatgtaacc ataaccacca gctggaatca cttgtgaagc cgcgatttct aaatcttcca
    22381 tattgataaa atctactatt ccttcatttg tacctgcttg atatgttttt tccatctcaa
    22441 tgccttctct ctataaaatt tgaatgatga acaatatgat agcgtttaca ctttttatat
    22501 tactcttcga cattgataca ggcaaataat ctgtttacat ttcagccatt ccagatgtca
    22561 ctactctgga agaaatgatt gtaataaaaa tagataaaaa accgatggga ctttgacaaa
    22621 agtagtgtca aagtcccttt ttttgaataa acggtgttca aaagccattt tctcggtatt
    22681 aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
    22741 aactaagcga ctttttcaca acctctcgat
//
LOCUS       DASJOX010000036        22757 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJOX010000036 DASJOX010000000
VERSION     DASJOX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.036"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..935
                     /locus_tag="U4A12_001292"
     CDS             123..935
                     /locus_tag="U4A12_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN1773662.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="U4A12_001293"
     CDS             932..1732
                     /locus_tag="U4A12_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN1773663.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="U4A12_001294"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="U4A12_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN1773664.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="U4A12_001295"
     CDS             3229..4938
                     /locus_tag="U4A12_001295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN1773665.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5150..9508
                     /locus_tag="U4A12_001296"
     CDS             5150..9508
                     /locus_tag="U4A12_001296"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN1773666.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9653..10126
                     /gene="rimP"
                     /locus_tag="U4A12_001297"
     CDS             9653..10126
                     /gene="rimP"
                     /locus_tag="U4A12_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN1773667.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10149..11324
                     /gene="nusA"
                     /locus_tag="U4A12_001298"
     CDS             10149..11324
                     /gene="nusA"
                     /locus_tag="U4A12_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN1773668.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11346..11639
                     /locus_tag="U4A12_001299"
     CDS             11346..11639
                     /locus_tag="U4A12_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN1773669.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11636..11947
                     /locus_tag="U4A12_001300"
     CDS             11636..11947
                     /locus_tag="U4A12_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN1773670.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11960..14266
                     /gene="infB"
                     /locus_tag="U4A12_001301"
     CDS             11960..14266
                     /gene="infB"
                     /locus_tag="U4A12_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN1773671.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14293..14640
                     /gene="rbfA"
                     /locus_tag="U4A12_001302"
     CDS             14293..14640
                     /gene="rbfA"
                     /locus_tag="U4A12_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN1773672.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14797..14970)
                     /locus_tag="U4A12_001303"
     CDS             complement(14797..14970)
                     /locus_tag="U4A12_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773673.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15418..17780
                     /locus_tag="U4A12_001304"
                     /pseudo
     CDS             15418..17780
                     /locus_tag="U4A12_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18148..19071
                     /gene="truB"
                     /locus_tag="U4A12_001305"
     CDS             18148..19071
                     /gene="truB"
                     /locus_tag="U4A12_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN1773674.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19075..20016
                     /gene="ribF"
                     /locus_tag="U4A12_001306"
     CDS             19075..20016
                     /gene="ribF"
                     /locus_tag="U4A12_001306"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN1773675.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20368..21153
                     /locus_tag="U4A12_001307"
     CDS             20368..21153
                     /locus_tag="U4A12_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1773676.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21166..22017
                     /locus_tag="U4A12_001308"
     CDS             21166..22017
                     /locus_tag="U4A12_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN1773677.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22141..22593)
                     /locus_tag="U4A12_001309"
     CDS             complement(22141..22593)
                     /locus_tag="U4A12_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773678.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7736 a         3965 c         4966 g         6090 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg
     3841 aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg
     4621 ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5821 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5881 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5941 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     6001 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     6061 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6121 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6181 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6241 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6301 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6361 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6421 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6481 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6541 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6601 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6661 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6721 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6781 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6841 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6901 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6961 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     7021 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     7081 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7141 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7201 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7261 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7321 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7381 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7441 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7501 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7561 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7621 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7681 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7741 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7801 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7861 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7921 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7981 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     8041 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     8101 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8161 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8221 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8281 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8341 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8401 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8461 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8521 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8581 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8641 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8701 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8761 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8821 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8881 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8941 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     9001 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     9061 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9121 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9181 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9241 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9301 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9361 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9421 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9481 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9541 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9601 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9661 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9721 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9781 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9841 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9901 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9961 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
    10021 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
    10081 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10141 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10201 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10261 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10321 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10381 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10441 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10501 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10561 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10621 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10681 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10741 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10801 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10861 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10921 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10981 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    11041 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    11101 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11161 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11221 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11281 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11341 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11401 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11461 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11521 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11581 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11641 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11701 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11761 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11821 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11881 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11941 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    12001 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    12061 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12121 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12181 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12241 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12301 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12361 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12421 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12481 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12541 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12601 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12661 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12721 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12781 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12841 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12901 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12961 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    13021 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    13081 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13141 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13201 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13261 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13321 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13381 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13441 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13501 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13561 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13621 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13681 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13741 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13801 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13861 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13921 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13981 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    14041 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    14101 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14161 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14221 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14281 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14341 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14401 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14461 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14521 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14581 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14641 tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa
    14701 gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag
    14761 agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat
    14821 atagataaag atcaagatac cgataacgga agctagattg taatctattt tagtgacagt
    14881 caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt
    14941 cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc
    15001 gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct
    15061 gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc
    15121 aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt
    15181 cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa
    15241 cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt
    15301 tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg
    15361 tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg
    15421 gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat
    15481 ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc
    15541 gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt
    15601 tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg
    15661 ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag
    15721 atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca
    15781 tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc
    15841 ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta
    15901 atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg
    15961 ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta
    16021 aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca
    16081 aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa
    16141 ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa
    16201 aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc
    16261 gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga
    16321 accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat
    16381 gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt
    16441 attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct
    16501 atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact
    16561 ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc
    16621 gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca
    16681 aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc
    16741 gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg
    16801 gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg
    16861 caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga
    16921 tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa
    16981 gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga
    17041 cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata
    17101 cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct
    17161 cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg
    17221 aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca
    17281 ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg
    17341 aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc
    17401 atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag
    17461 acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg
    17521 atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac
    17581 catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt
    17641 taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag
    17701 aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat
    17761 ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttacatt
    17821 gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag aatggccagt
    17881 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
    17941 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaagcataa
    18001 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
    18061 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
    18121 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc
    18181 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
    18241 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
    18301 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
    18361 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
    18421 gatatgtctt tatctgaaaa aaaaatagat gaagtgatgg cttcatttat tggtgagatc
    18481 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
    18541 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
    18601 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
    18661 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
    18721 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaggat
    18781 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
    18841 ctgccaatag aaaaaggagt ggaacggttc aaacgagtag atattactga tgaagtatgg
    18901 cagaaagtaa aaaacggtat gagacttgat tatactgtat ttgcgctccc agaaatgccg
    18961 tcagaagaaa ttgctttgtt ttataaagga aaagttgtca gtatttatca gccaaacccg
    19021 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
    19081 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
    19141 gtgctgggat ttttcgacgg cgtgcatatc ggacaccaga aagtcataaa gacaggtatg
    19201 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatct
    19261 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
    19321 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
    19381 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
    19441 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
    19501 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
    19561 ggtgaaaaaa tcagctctac ccgtattcgt ggattactag acagcggaca agtaaaagaa
    19621 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
    19681 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac ggttcatttg
    19741 cctaaagaag gtgtttatgt gtcggaaata aaaattgggg aaacatggta tccagccatg
    19801 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agagttgtat
    19861 attttggact ttaatcaaga tatctatggt gaacatgtcg cggtacgctg gaatcaattt
    19921 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaggaggat
    19981 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
    20041 ttatgtataa ctagcaatta caataaaatg ggtgataaat aagcccagga aaggggctgt
    20101 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
    20161 cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct ttctttatag
    20221 aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat ttgctttaga
    20281 ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg taataacagg
    20341 tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa agtcgcagta
    20401 gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg gaaagaaaaa
    20461 atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc agtcaaagca
    20521 atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc tgaagaagga
    20581 atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt ttgggttaat
    20641 aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga ttgggaaaga
    20701 gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt aaattacttt
    20761 acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga acagatccca
    20821 tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt taccgaaacg
    20881 gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc tggtgctatc
    20941 cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact cttggaaagt
    21001 atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc tgcatggttg
    21061 gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg tgggatgact
    21121 ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg tttattgatt
    21181 gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa gattggtggc
    21241 agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg cttactcgtt
    21301 caattattcg cttctccagc tggtatttca gtgactacat ttttgattag tttactttct
    21361 ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt gattggtgtt
    21421 tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt gattggtgtc
    21481 ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc agcaatcatt
    21541 cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa aaaaggcggt
    21601 atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt atacagtgca
    21661 ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat gatcggtgtc
    21721 tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc tgaagacaag
    21781 agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc ttatattttt
    21841 tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa cgtagttatc
    21901 tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga aaaaaaatta
    21961 acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt tatttgagga
    22021 taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac agtaagtact
    22081 tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct ttttaattgt
    22141 ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc attgactaaa
    22201 gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt ctttataaag
    22261 atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt actggactaa
    22321 ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga aacgcatcgt
    22381 gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg cttgatctcc
    22441 gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa cctttgttcc
    22501 ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac ctaattgttc
    22561 ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt gaatggttct
    22621 actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt taattaaggt
    22681 cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa ttcgttctct
    22741 cctaaagtga tattata
//
LOCUS       DASJOX010000037        22172 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJOX010000037 DASJOX010000000
VERSION     DASJOX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.037"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            42..1940
                     /locus_tag="U4A12_001310"
     CDS             42..1940
                     /locus_tag="U4A12_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN1773679.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2239..2958)
                     /locus_tag="U4A12_001311"
     CDS             complement(2239..2958)
                     /locus_tag="U4A12_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN1773680.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3295..3627
                     /locus_tag="U4A12_001312"
     CDS             3295..3627
                     /locus_tag="U4A12_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773681.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3614..5617
                     /locus_tag="U4A12_001313"
     CDS             3614..5617
                     /locus_tag="U4A12_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN1773682.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5636..6106
                     /locus_tag="U4A12_001314"
     CDS             5636..6106
                     /locus_tag="U4A12_001314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN1773683.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6127..6711
                     /locus_tag="U4A12_001315"
     CDS             6127..6711
                     /locus_tag="U4A12_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1773684.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6726..7712
                     /locus_tag="U4A12_001316"
     CDS             6726..7712
                     /locus_tag="U4A12_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN1773685.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7702..8013
                     /locus_tag="U4A12_001317"
     CDS             7702..8013
                     /locus_tag="U4A12_001317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN1773686.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8035..9816
                     /locus_tag="U4A12_001318"
     CDS             8035..9816
                     /locus_tag="U4A12_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN1773687.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9809..11185
                     /locus_tag="U4A12_001319"
     CDS             9809..11185
                     /locus_tag="U4A12_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN1773688.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11192..11827
                     /locus_tag="U4A12_001320"
     CDS             11192..11827
                     /locus_tag="U4A12_001320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN1773689.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11857..13212
                     /locus_tag="U4A12_001321"
     CDS             11857..13212
                     /locus_tag="U4A12_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN1773690.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13404..14798)
                     /locus_tag="U4A12_001322"
     CDS             complement(13404..14798)
                     /locus_tag="U4A12_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1773691.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14890..15861
                     /locus_tag="U4A12_001323"
     CDS             14890..15861
                     /locus_tag="U4A12_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1773692.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15858..16640
                     /locus_tag="U4A12_001324"
     CDS             15858..16640
                     /locus_tag="U4A12_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN1773693.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16809..17180)
                     /locus_tag="U4A12_001325"
     CDS             complement(16809..17180)
                     /locus_tag="U4A12_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1773694.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17314..17757
                     /locus_tag="U4A12_001326"
     CDS             17314..17757
                     /locus_tag="U4A12_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN1773695.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17800..18609)
                     /gene="yidA"
                     /locus_tag="U4A12_001327"
     CDS             complement(17800..18609)
                     /gene="yidA"
                     /locus_tag="U4A12_001327"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN1773696.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18628..19995)
                     /locus_tag="U4A12_001328"
     CDS             complement(18628..19995)
                     /locus_tag="U4A12_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1773697.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19979..20809)
                     /locus_tag="U4A12_001329"
     CDS             complement(19979..20809)
                     /locus_tag="U4A12_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1773698.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20972..21409
                     /locus_tag="U4A12_001330"
     CDS             20972..21409
                     /locus_tag="U4A12_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN1773699.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21485..22105
                     /locus_tag="U4A12_001331"
     CDS             21485..22105
                     /locus_tag="U4A12_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN1773700.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7109 a         3848 c         4781 g         6434 t
ORIGIN      
        1 actttataat gttgagtata ccaatttttg gaggcgtttt aatggaccgc tatttagatt
       61 tactttcaga gaagttccct acaacggaag atgtcatcac ggaaatcatc aatctcgaag
      121 caattttgca gcttccaaaa ggaacagagt tatttttgag cgatatacat ggcgaatttc
      181 ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag atcaaagacc
      241 ttttctctga acaaatgacg gaaacggaga tcagccaatt cactattttt acggcttatc
      301 cagaatatgt gttgacgact gattggtaca aaaaacaaga taaatcccag ctgatccatc
      361 gtttgatcga tcttttacgg tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag
      421 gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat gatcgtatca
      481 acgggaaaaa agactatgta aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag
      541 aaaaattttt aacaaaactt gcgcagacga tccaaaaatc tgtcatcgat catttgcata
      601 tcgtgggtga tatttttgat cgaggaactc aagcagccaa agtaatggat cgaatcatgg
      661 atttttcctc tgtagatatc cagtggggga atcatgatat tttgtggatt ggtgcgtatt
      721 gcggctctga ggcatgttta ttgacgcttt tacggattgc cgcacgctac aactatttat
      781 ttgaattaga gaaagaatat ggattaaatt tacgtccatt attttctttt gcccatcaaa
      841 cctatcagaa gaatccagta tttaccccta aaaacaggaa agatctatct gaaagagagc
      901 aagaagaatt agaaaagatc catcaagcac tttctatcct ccaattcaaa tcagaacatc
      961 agttgttgaa tcgacgacct gaatttaaaa tggacgatcg taaattgttg gaaaaaatca
     1021 actatgagga atcaacgatc gttttagaag gtcagctcta tgagttgaaa gggacttgtt
     1081 ttcaaacgat ccagccagat gatcctaaaa aattcttgga agaggaacaa aaagtgatcg
     1141 atagtttgat gtattcattc cagcactcgt taagaatgca aaaacacatg acgtttttga
     1201 tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt actgttccat ggctgtatac
     1261 ctgtagatga aaaggggcag ctcttagcat ttgaattaga ccaatcttat cgcggaaaag
     1321 aactgctggg attttttgaa aaacagataa ctgaaagcgc aaaagatttg actgcaaatg
     1381 aagatttggc gaccgatttg atttggtatg cgtggagcgg gccattttcg cctttattcg
     1441 gcaagagtaa aatggcaact tttgagcgct attttctcac agagcctcat acgcacgtcg
     1501 aaaaaagcaa tccctattat cacttgcgag atgaagagtg gttcagtgaa aaaattttgg
     1561 cagaattcga tgcaaaagaa gaaggttcag caatcatcaa tggacacacg ccagtaaaag
     1621 taaaaaaagg agagtcaccg atcaaagcaa acggaaaagc atttgttatt gacggaggtc
     1681 tatccaaagc ttatcaaaaa acaaccggga ttgctggcta ctcattacta tgcaattctt
     1741 acggtttcca aatcgtgaca cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa
     1801 aatctgatca aacgaatgta aaaaaagtga tcgagcagca gctcccacgc aaacttaatc
     1861 ggaatacgac aaaaggaatg gaattacagc aacagattac tcaactccaa aggctgttgg
     1921 attatcgaaa ggacgattga gaaaaatgct agccaacatt gctcaataga ctaaaaagcg
     1981 actgcaacaa aaggcgtgtc gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat
     2041 tagaagctat ttttctgttt atttctctaa tactcaggtc agggcgctga tttcacgacc
     2101 tcttccttaa acggtgttct cctgccaact cctcggtcac aagtcacaat atccaacaaa
     2161 catgagaagc tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg
     2221 acctaatcag aaaaacgatt aaaattcatt cacaaagaaa tcgaataaac gctgttcaga
     2281 tgaatctaca cgatgagaaa gttccggatg ccattggaca ccaaggattt tgtgcatatt
     2341 atctttactt tggactgctt caatgagtcc atcgttggaa aaggcgatgg cttttaacaa
     2401 aggagaaaga tctttgactg cctgatgatg ataggaattc acaaagtatg aatcagggag
     2461 aaggtcactt aataaactgt ccgaaacgat tttgacttcg tgagttgcaa actgcggggc
     2521 agtgggctgt tgttcgtgtt ttacggacca ttttggatat aatgaaagat cttgataaag
     2581 tgttccctcc aacacgacat tcaatagttg aagtcctcgg cagactgcaa aaatcggttt
     2641 tctttgtttc aatgcttcca agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt
     2701 ttccgtcagt tttggatgag ggtcttccat gtaaagttgc ggactaacat cctgaccgcc
     2761 tgcaagaatg agtttgtcga ttttctcaat tagttgaggc gctgaatctg gagacatgac
     2821 tggcagaata agaggtaatc cttgagcatc taaaactgca tccacaaatc cctgcggtgt
     2881 atagcttact tgatttcctt ggaaagtatc tgtcgctcga atcagttgat tcccggcaat
     2941 gccgatgatt ggtttcattt ttattccccc taaagtagac taattgtaac aaggatttaa
     3001 aaaaatttaa actatgtaag acttgcccct atacttatct gttcttgctg tgttactatc
     3061 tgtaaagaga attttcgtgt gaaattcatg acgatagttg aaaatattgc ttaatttgtt
     3121 ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc
     3181 aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat ctttagaggt
     3241 acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag
     3301 caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga gaagcgtgct
     3361 cgaaccctac ggcagcagtt gaagacctat gcagaagaaa aagagcaaga attggagata
     3421 atccaaacga acagtcggca acagctgaaa cagttggtag aagaaaaaga acaattcgaa
     3481 aagcaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca
     3541 actttgaaag accaatatca aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg
     3601 gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt cagataaaag
     3661 aaatcaggag aggattttac aagcagtaca aggaatccaa aatgttgaaa tccgggatct
     3721 gtatcaaaat agaacaaaca accagtgggt agaaaaatat tttccagata ccacgattat
     3781 tgataaagaa gcaaaattaa gcgctttagg caatcggctg aaagacatcc aagaagctat
     3841 ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga
     3901 actttcctta caagacttgg aagcaaatta ttccgaagaa aatttcatga ataaattaga
     3961 agaagtcctt gatttaaaag aaaagtggga acagctaact gaaaaacgtg atcgtttaga
     4021 agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa gtatgtatgc
     4081 aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg aaagtttcaa
     4141 ggaagaacta agtaagcttg atgacgttta tatggaagaa atagattata cgccaaaaca
     4201 agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gctatagaag aaattgctgg
     4261 gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca
     4321 agaaaccaaa cagcaattat ccattgttca cgagcagcaa aaaaagataa cgacagcgtt
     4381 aggtcagtgc agcggctata tccaagaatt tgaatgggta gaagaagtca cacttgccgt
     4441 tgcagaacgg gaaaaagtaa aagaacggtt tatccatgcc acttatctga tcgttttgca
     4501 aggttgggta gatgcagaag aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc
     4561 agaagaagat atatacgtta gtttcgaaac accaaactca gaagagattc agcaggaagt
     4621 ccctactaaa ttgaaaaacc atccgcttat cgaacctttt gaattgctga ctgaaatgta
     4681 cagtctgcca aaatatgaag aagtagatcc aacgccttgg atgacaccat tttatcttgt
     4741 cttcttcggg atgatggtcg cagatgttgg ttacggattg ctgatgctca ttgggacaat
     4801 actagctcaa aaactatttg ttttaccccg cgggatgaaa cgtttcgtaa aattctttga
     4861 gattctctcg gtaccatcga tcatctgggg attgatctac agttcatttt tcggacaggc
     4921 attaccaaaa gaactatttg gtatccgctt gcctttccca atcctatcta cgacggatga
     4981 cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagatct tggttggatt
     5041 attcgtggca gcaaaggaaa atatcaggag gaaagattac ttgagtgctg tcagtgatgg
     5101 ttttgcctgg caagggatcc tgattgggat cgttattgca ttgattggtg tcatgttgat
     5161 aaaaaataca gcatttgttt atcttggcgc ggggctatcg atcattgctg ctttatgtat
     5221 cttaatcgtg ccgattatcc aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta
     5281 taatctttac ggattgactg gttatatcgg tgatttagtc agttatacac gtttgatggc
     5341 actaggtatt tcaggcggaa gtattggcgc ggcattcaat atgctagttg ctttcatgcc
     5401 gccagccgct cgattcagtg tagggatcct tttgatcatc gctttacacg cactgaatat
     5461 gtttttgaca ctgttaagtg cctatgtcca tggtgcacga ttgcagtatg tcgaattttt
     5521 tggaaaattt tatacaggag gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata
     5581 cgtcaacatc aatcataaga aaaaaaacaa taaataattg gaggattttt atacaatgat
     5641 ggattactta attagtcaaa atggtggaat ggtatttgca gtattagcga tggcaacagc
     5701 gacgattttt tcagggattg gttcagctaa aggtgttgga atgacaggtg aggcagcagc
     5761 agcgttaaca acaagtcaac ccgaaaaatt cggacaggct ttgattctgc aattgcttcc
     5821 aggtacgcaa gggttgtatg gattcgttat cgcctttttg atttttatca acttaggaaa
     5881 tgatatgtca gttgtacaag gattgaactt tctaggcgct tctctgccga ttgcttttac
     5941 aggtttgttt tcaggtatcg cccaagggaa agttgcagca gcaggtatcc aaattttagc
     6001 gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg aaacatatgc
     6061 gattttaggt ttcgttattt ccttcctatt agtattgaat gcctaagcat aaaggaggaa
     6121 aagcagatga acgccattga aaaaatcatc tctcagatga atgaagcagc agagcaagaa
     6181 agagcggcgt tagagcaaga agagcgaatg aagatcgatc aaaactttga acaaaaacgt
     6241 acgcaaatcg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa
     6301 aaataccgtc agcttaggaa tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg
     6361 aagcaggaat ttttacgacg attgtttgca gatgcaatag ctgaaatgga gaattgggat
     6421 gaatctgaac aaatccagtt tatcaaaaat tcattgtatt cattaccttt gacaggcaaa
     6481 gtaacattca ttgctggaga aaaatcagca gcttatcttt ctcagacatt gctggatgaa
     6541 tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg
     6601 ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag ccttgtacaa
     6661 gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata aggagggatc
     6721 agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt gaacttttgt
     6781 ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt ggtgaaatat
     6841 tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac ttcgaacaga
     6901 atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca ccggagcctg
     6961 aaatcgtttg gatctatacg atgcgctata ctttccataa tctgaaggtt ttgacaaaag
     7021 ccgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt tattcattag
     7081 aaaccttgaa ggatgccatt catacgcaag tttcaaatga actccctgaa agcattatgg
     7141 acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag ggaattgacg
     7201 tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag ctaggatatc
     7261 aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca acgatggctc
     7321 gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc agctcgggaa
     7381 gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat tacacgaatt
     7441 ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag ggaaatcttg
     7501 atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat caagaagcac
     7561 aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa gaggtagaaa
     7621 gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaacca gaaacaatca
     7681 gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga caaagattct
     7741 gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt
     7801 cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt cacagagcaa
     7861 tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat acctgctatc
     7921 gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat tcaaaaaaac
     7981 gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga aattttgcaa
     8041 attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat gtctgatgcc
     8101 agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat tatcgagatg
     8161 cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg tccaggcgaa
     8221 ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat catttcacaa
     8281 atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca aagtaatttc
     8341 ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg gttcgaacca
     8401 acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca
     8461 aaagttataa aacataaaat catggtgcca aatgggataa aaggaacgat caaaacaatc
     8521 gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac
     8581 aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat caaagaaaaa
     8641 ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt cttcccagtg
     8701 acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac agtcgttcag
     8761 catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg tggcgagcgt
     8821 ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc taatacagga
     8881 gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc agtagcagct
     8941 cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga catgggttat
     9001 gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg tgagatgagc
     9061 ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg ttcacggctt
     9121 gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg tcgcgaagga
     9181 agtataaccg caatcagtgc tgtatccccg tctggagggg atatttcgga accagtcaca
     9241 caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt agcccaaaaa
     9301 agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac agaagtaggg
     9361 aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga gggcatgcgt
     9421 attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat tgattccctt
     9481 tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag
     9541 caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt caaaatgttg
     9601 aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta
     9661 agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag caaatatatc
     9721 ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca aacgatgcaa
     9781 acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga tcggtgaagt
     9841 cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga
     9901 agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc aagaagataa
     9961 agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact cttctgtacg
    10021 atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc gagtattcga
    10081 tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc atttggatat
    10141 taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta tccaaacagg
    10201 tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac cagtattttc
    10261 tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag caacggtact
    10321 cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc
    10381 tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat
    10441 gaatttagca aacgaccctg caatcgaacg tatcgctact cctcgtatgg ctttgactgc
    10501 tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga cagacatgac
    10561 caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc caggaagaag
    10621 aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag caggaagaat
    10681 ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag aagacgataa
    10741 aactcatcca atccctgact tgactggtta tattacagaa ggacagatca tcttgactag
    10801 agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc tttcgcggtt
    10861 aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta cgatgaatca
    10921 gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga
    10981 atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga
    11041 atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg atctagggtg
    11101 ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg acatgctgga
    11161 caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga atccgacccg
    11221 catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc ataaattatt
    11281 aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa agaacaacca
    11341 gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg tattggcaaa
    11401 tgcttcgatc acggaacctt tcatcgaaga attatttgtt ttgccggcgg aaaacgttga
    11461 tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga attttgaata
    11521 tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca gtgccgaact
    11581 ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac tgacagaaat
    11641 cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc
    11701 attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc gtatgaagct
    11761 ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac
    11821 tgactaagtg gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag caaacaaata
    11881 aagaaacgtt tgccggctgt tcagcttctc gcattaggat tttttctcct gattttagtt
    11941 ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac aaattttatc
    12001 gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac attgaataca
    12061 gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga gatcggcggt
    12121 cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa agtcagtctc
    12181 agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg tgtgatgaag
    12241 ctcatgcttt acatcctaaa attggccttt gttatccaat tgattggtac ggttgcttta
    12301 tcttttgtat ttgtgcctga atttggctgg ttgaaaggta tctggtacag tttgttccat
    12361 gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt agccaatcac
    12421 cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg tgggttaggc
    12481 ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagatgac gattcattca
    12541 aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt gttctttttc
    12601 acagagcgca atggggcaac gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc
    12661 ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta cctccatatg
    12721 agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggcggaac atctgggtcg
    12781 acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat gcgttcgatg
    12841 ttgaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg cgcagtgttg
    12901 cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat catgatttta
    12961 tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt tgaggtcttc
    13021 tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac tgcaataggg
    13081 aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac tgtggtcttc
    13141 tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga agaaagtgtc
    13201 atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat aagaaaataa
    13261 aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat tttttgaggc
    13321 actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat caataagatt
    13381 tgattccttt atttatcaat aggttataat tcatattttt ttattttttc ttttacgata
    13441 cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg aacgaatgca
    13501 tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc gttagcaaga
    13561 aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa
    13621 gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg attggctctt
    13681 tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg gaggatttga
    13741 gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat tgcttgttct
    13801 atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc agaacaaata
    13861 aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg gaaaggagga
    13921 atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc aataacccat
    13981 agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac
    14041 cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt ttttctacat
    14101 tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg aacgatctct
    14161 aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt
    14221 ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg aacaccggaa
    14281 acaatagata gagattctaa ggacggaatg actccttgag tttttaacca atttgctgca
    14341 gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc aacaagattt
    14401 gggttattac tagttgattg aataaacggg acaacgaatt tattacattc ttcaaaagaa
    14461 ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat ggtgatggac
    14521 tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag caaacctttc
    14581 tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa ataatcagct
    14641 agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa aatatcttgt
    14701 tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt gtctggtcgc
    14761 caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc ttaaaattgt
    14821 ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt tgtgaggagg
    14881 gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag ttttgaagaa
    14941 gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat cagccaaaat
    15001 cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc gatgatgcct
    15061 gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt tccggataat
    15121 catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc tcaaaacgga
    15181 ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct
    15241 gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt aattggagct
    15301 ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat cactacagtg
    15361 tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc taaaaaacag
    15421 tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg tccagcggaa
    15481 atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa
    15541 gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat aagtacatcg
    15601 ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac agaaagcggg
    15661 gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt tggtacatta
    15721 ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc gacgattttc
    15781 gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta taacaaatta
    15841 gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt gcagatgagg
    15901 tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca gaaggtacga
    15961 tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg aagaaaaacc
    16021 aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt ggtcacgcga
    16081 cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa acgattaaat
    16141 tttctactca aagcggtaat ctatttgtga caagaattaa agattattat tacatggatt
    16201 ttcctagtat tatgccgaaa aaagtgccga tattgtctga atatgaagaa gcgattgggg
    16261 ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa gatgaaaaaa
    16321 cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta ggaataggag
    16381 tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc tagaaccttc tttccaaaat
    16441 taacaatcaa tgaagatcca gtatgtggat cagcacattc taatcttatt ccttattggg
    16501 cagaaagaat gaataaaaca aaattgtcag cttatcaaat gtcatcaagg ggcggggatc
    16561 tagaatgtga attaaaagaa gatcgtgtga taataggagg aaaagcagtt ttgttttcta
    16621 aaggtacctc gtttatttga tttctttcaa atgaattaaa aataaattgg tggagagtgg
    16681 caaccttata gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa
    16741 gtttgcgtct cattttaatt attggtgttt gttttttatt caatcaagag taaaatgccc
    16801 catactaact atcctcggat gacttcgatt ttataaccat cagggtcaat gacaaagtag
    16861 taagaaggcg cagtgccagg taatcctttt aagtcagtaa tctcaagacc ggcagcttta
    16921 tgttgctcgt gtaatttttc tagatcatca gctgctatag cgatatgacc atagccgtct
    16981 cctagatcat acgcaccatg atcatagttg taagtcaact ctaattcata gtcatcccca
    17041 ggaagtgtca aataaacaag tgtaaatttg ttttctggaa aatcacggcg gcggctttct
    17101 tcaaaaccaa atgccttttg ataaaattcg attgatgctt caagatcttt cactcgaaca
    17161 catgtgtgag ccattttcat agattcatct ctccttttcc ttttactcaa tcataacaaa
    17221 aagaagtaag gaaatagttg atttgtgctt gttcatgaga aatttctcat gtaaaatgga
    17281 tagaaaataa aactgttaag gtggaaacta ttcatggata aaccaatttt tggtgaaaaa
    17341 aagcaatttg ctgattatca aacccgttat gcagcatata ttgtcataga aagagatcag
    17401 cagattgcag taatcgaggc tccaaatggt gctttttttc ttcctggcgg ggaaattgaa
    17461 ggagaagaga cgaaagaaga agcgattgtc cgtgagctga tagaagaaat gggaattgtg
    17521 gcagaaattt cttcttatct gggagaagct gatgagtatt tttactcgaa ttatcgtgca
    17581 acttattatt ataatccagg atatttcttt gttgctgatt cttggaagcg tataggagaa
    17641 ccaactgaaa agaccaataa aatttggtgg gtgagccctc aagaagcgct tgtgaaattg
    17701 aaacgtggta gccaccgatg ggcagtcgag agatggatcg aaaaaaataa aaactgaaag
    17761 aaagcgagta gatcagcttt ctctcagttt ttttatttat tacagtacat attttttgat
    17821 tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg acatttgctg cttctttgac
    17881 aagagaaacg gcatttccca ttgctactcc tagaccagca tattcaatca tggataagtc
    17941 gttctcgtta tctccgatag ccatgatttc atcttgatca ataccaaggt gctgtgcgag
    18001 attcgcaact gccgcacctt tacttgcttc tttatttagt acttcgaagt aatagggcgc
    18061 acttttgacc gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc
    18121 taaaatttct ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc
    18181 ttcaggtgtt cgatatttta gcggcatctt gactaaactt gcttcgtgga tcgtataagc
    18241 gctgatatct cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc
    18301 gacacggcgt gccatcactt cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc
    18361 gatgatttta cctgatttcg tttgttgtac gagtgccccg ttataagtaa taacgtaatc
    18421 attatcttga aaaaggttca actcgtttaa ctgttcctgg acacctggta agggacggcc
    18481 agtgcataaa acgatcgaaa caccttttgc aacagcagcg gcaatcgctt cttttacttc
    18541 ttttgttact tctcttttct catttaataa agtaccatcg atgtcgatag cgactagttt
    18601 gatagacata atttcctcca atatatttta atttatttca atcaatgcgc cattacggat
    18661 atggctggca aattcttgat aagtttcgtc aaataaatca taatgatcac gcaaggacgg
    18721 atcaaccatt tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc
    18781 agcaacaagc tgactaacct gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc
    18841 gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa tcataaggta gatcgtagct
    18901 agaattcaca gcagtgtaat aaacaggatt atatccgacc ttttctacta acaaagtgag
    18961 ttcttggact aattttgaat cccggttgcc agaaaaagta gcagatttca gtggtttacg
    19021 attcaaaaaa cgtttagcta aatcatttaa aataggatca gcagattcag accattgagt
    19081 aaagtaagtg cccaagaccc catcatcaag tttgaggtat tcttctaaag taaagcttcc
    19141 gtcaagaaat ggcagaagta attgagaatg gagttcaaaa ctgctggcat cttcttgata
    19201 caactcgtgt gcgcgatgaa gcagatgctc caaaattact tccataccac gggaaacagg
    19261 atggaagtac acttgcacat acatttgata tcggctaacg atataatctt ccactgcatg
    19321 catgccactc atggaaaaag cgatcccgcc ttcataagga cgaatcacac gtaatattcg
    19381 ggtaagatca aatgttccat attctgttcc agtgaaataa gcatcccgaa gcaaatagtc
    19441 cattcgatct gcatcgatct gactagaaat catttggaca acttgtgggt tggggtaagt
    19501 tttttggatc acactagcta ccttttcagg aaaaccttct tctactcggt taagaatctg
    19561 atacacttct gtttctggag aagtgatgat ctctacagtg atggcttcat gattcgtatg
    19621 gaagatatgt tcaaatgtat gagagtaagg accatgaccg acatcatgca acagtgccgc
    19681 acataaagtc accagccgtt cctgatcatc ccagcctccg ttgcctattt tttcttttga
    19741 aaaatttcga gaaaagatat cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg
    19801 agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat
    19861 gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct aagattactt gatgctggac
    19921 atggatatag ttatggactg gatcacgaaa aactttttcc ataggcagca tttgatattt
    19981 ataaggggtt gtcataatgt agctccttta tgttattacg ttgttccatt ctctggattt
    20041 ggttgttcca ttgctccagc tggatttctt tttttagcca gtcggtagaa tcctgtaaat
    20101 cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa
    20161 aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc actgcaggat
    20221 agccatttgt accaaagtct tcttttaaag cagattgata aaatcgcttc atgacttgcc
    20281 cgcgtttttc ctgattaccg ttgacactta agtacatcat aacagaaatc ccatttttga
    20341 tccgacgctg agcgataccg gcaaattttt tgccatggat gcttaaatca taagttccag
    20401 gacaataaga atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag
    20461 tcaataggag catttgctcg taagcgtcat ctatggttat tttttttgtg tcactttggg
    20521 ggaaaatcaa agaaatattc aaaattcctt catctgatac gatccctaat ccacctgaat
    20581 ttctgatcac tggagcgtaa cctgcatctt tcagtgtttg gatcccgtca cttagaaaag
    20641 gagttcgaac atccttcata cctaagatga ctgtgcagtc atattgccag aaatgtaaaa
    20701 agatttgctg gtgttcttta gaatattccg ttaatatatc tgttaatgca aaaggggaaa
    20761 agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc agggttcatt caaccggctc
    20821 ctttgtataa attcatattt tctacaatat tatactctaa aatcaaagag ttgttttgga
    20881 aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa
    20941 tgtagctatg agaagtaaag gagaatgaat aatggaatta tcacaaggaa caccgatttc
    21001 aatcaaattg cgtacaaaag taaaacagca aggggaagtg caagattttt attttgattt
    21061 gaaagggcaa atggtgacaa ttggcgatac gctgtacata cgttataaag agattcagga
    21121 ggagacgggc gatgagattc ctgtaacgat caaattagtt ccagatggtc atgtacagct
    21181 gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc tataaagaac ggttagaaac
    21241 atcctatcga acaccatacg gtatgatcca gattgctact tttacgaaag aactgcatgt
    21301 tagtttgaaa gaccgtccca caggagggaa ggttcgaatc gattatgatc tgttcatggg
    21361 accggaaaaa atcggcgaat attatttgac cttggatttt acagcgtaaa tataaaagaa
    21421 aagtttgatt ttttgtgtct gattttgtat gataaggagt agactgtgaa aggacgtgtc
    21481 ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt
    21541 agcacacgcg atcttagaac aacgtggcga tgtcatggat ttttctgatt tagctaacca
    21601 gatccaaaac tatcttggga aatcagacag tgaaattcgt gattcactcg cacaatttta
    21661 tactgacttg aatattgacg gcagctttat ctcattaggg gataatcgtt ggggcttgcg
    21721 ttcatggtat gctatcgatt cgatcgatga agaagtaaac catggaatgg acgaagagga
    21781 tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca atgatgatga
    21841 agacgcgatc gattacaatg acgatgaccc agaagataca gatctgacag aagaagatga
    21901 cgatgatctg tttgatgacg atgatgatga agatgaagaa atcgctgctt ataattctga
    21961 cttgcaagaa atcggagcag atgacccgac agatgatgaa gaagacttgc caggaaatat
    22021 cgaagaagat ttaagtatca tagacgaaga cgatgatgat gaagattatg aagaggaaga
    22081 attttctgac tctgaaaaag aataaacatt attttcttac gagaggttgt gacagagacg
    22141 atcagcctat cagaataagg aggtgtgaca cg
//
LOCUS       DASJOX010000038        22026 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJOX010000038 DASJOX010000000
VERSION     DASJOX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.038"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..1371)
                     /locus_tag="U4A12_001332"
     CDS             complement(61..1371)
                     /locus_tag="U4A12_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1773701.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="U4A12_001333"
     CDS             complement(1383..2801)
                     /locus_tag="U4A12_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1773702.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="U4A12_001334"
     CDS             complement(3041..4609)
                     /locus_tag="U4A12_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1773703.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="U4A12_001335"
     CDS             complement(4613..4894)
                     /locus_tag="U4A12_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1773704.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="U4A12_001336"
     CDS             complement(4891..5814)
                     /locus_tag="U4A12_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN1773705.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="U4A12_001337"
     CDS             complement(5811..6689)
                     /locus_tag="U4A12_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN1773706.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="U4A12_001338"
     CDS             complement(6676..8013)
                     /locus_tag="U4A12_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1773707.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="U4A12_001339"
     CDS             complement(8016..9119)
                     /locus_tag="U4A12_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1773708.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="U4A12_001340"
     CDS             complement(9193..10701)
                     /locus_tag="U4A12_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1773709.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="U4A12_001341"
     CDS             complement(10667..11359)
                     /locus_tag="U4A12_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1773710.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="U4A12_001342"
     CDS             complement(11381..12355)
                     /locus_tag="U4A12_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1773711.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="U4A12_001343"
     CDS             12655..13527
                     /locus_tag="U4A12_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1773712.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="U4A12_001344"
     CDS             complement(14109..16307)
                     /locus_tag="U4A12_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773713.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="U4A12_001345"
     CDS             16539..17531
                     /locus_tag="U4A12_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773714.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="U4A12_001346"
     CDS             17591..19012
                     /locus_tag="U4A12_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN1773715.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="U4A12_001347"
     CDS             19005..20504
                     /locus_tag="U4A12_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN1773716.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="U4A12_001348"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="U4A12_001348"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1773717.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DASJOX010000039        21985 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJOX010000039 DASJOX010000000
VERSION     DASJOX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.039"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="U4A12_001349"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="U4A12_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN1773718.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="U4A12_001350"
     CDS             complement(2019..2921)
                     /locus_tag="U4A12_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1773719.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="U4A12_001351"
     CDS             complement(2918..3610)
                     /locus_tag="U4A12_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN1773720.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="U4A12_001352"
     CDS             complement(3653..4294)
                     /locus_tag="U4A12_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1773721.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4601)
                     /locus_tag="U4A12_001353"
     CDS             complement(4332..4601)
                     /locus_tag="U4A12_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773722.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4574..5263)
                     /locus_tag="U4A12_001354"
     CDS             complement(4574..5263)
                     /locus_tag="U4A12_001354"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEN1773723.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="U4A12_001355"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="U4A12_001355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEN1773724.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="U4A12_001356"
     CDS             complement(5983..6915)
                     /locus_tag="U4A12_001356"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN1773725.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="U4A12_001357"
     CDS             complement(6912..7688)
                     /locus_tag="U4A12_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN1773726.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="U4A12_001358"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="U4A12_001358"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN1773727.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="U4A12_001359"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="U4A12_001359"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN1773728.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="U4A12_001360"
     CDS             complement(12013..13293)
                     /locus_tag="U4A12_001360"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN1773729.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="U4A12_001361"
     CDS             complement(13312..14238)
                     /locus_tag="U4A12_001361"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN1773730.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="U4A12_001362"
     CDS             complement(14251..15534)
                     /locus_tag="U4A12_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN1773731.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="U4A12_001363"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="U4A12_001363"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN1773732.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="U4A12_001364"
     CDS             complement(16322..17233)
                     /locus_tag="U4A12_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1773733.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="U4A12_001365"
     CDS             complement(17230..17694)
                     /locus_tag="U4A12_001365"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN1773734.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="U4A12_001366"
     CDS             complement(17699..18397)
                     /locus_tag="U4A12_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1773735.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="U4A12_001367"
     CDS             complement(18384..18770)
                     /locus_tag="U4A12_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773736.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="U4A12_001368"
     CDS             complement(18917..20587)
                     /locus_tag="U4A12_001368"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN1773737.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="U4A12_001369"
     CDS             20770..21003
                     /locus_tag="U4A12_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1773738.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="U4A12_001370"
     CDS             complement(21102..21533)
                     /locus_tag="U4A12_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN1773739.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..>21985
                     /locus_tag="U4A12_001371"
     CDS             21612..>21985
                     /locus_tag="U4A12_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN1773740.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLAR"
BASE COUNT         6208 a         4918 c         3885 g         6974 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttcaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtca
//
LOCUS       DASJOX010000040        21911 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJOX010000040 DASJOX010000000
VERSION     DASJOX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.040"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4A12_001372"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4A12_001372"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN1773741.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="U4A12_001373"
     CDS             complement(1172..1867)
                     /locus_tag="U4A12_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN1773742.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="U4A12_001374"
     CDS             complement(1880..2596)
                     /locus_tag="U4A12_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN1773743.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="U4A12_001375"
     CDS             2926..3534
                     /locus_tag="U4A12_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN1773744.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="U4A12_001376"
     CDS             3859..4182
                     /locus_tag="U4A12_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1773745.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="U4A12_001377"
     CDS             4364..5734
                     /locus_tag="U4A12_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN1773746.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="U4A12_001378"
     CDS             complement(5864..7165)
                     /locus_tag="U4A12_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1773747.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="U4A12_001379"
     CDS             complement(7519..9477)
                     /locus_tag="U4A12_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN1773748.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="U4A12_001380"
     CDS             complement(9654..9920)
                     /locus_tag="U4A12_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1773749.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4A12_001381"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4A12_001381"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN1773750.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4A12_001382"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4A12_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN1773751.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="U4A12_001383"
     CDS             13264..13827
                     /locus_tag="U4A12_001383"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1773752.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="U4A12_001384"
     CDS             complement(14080..14601)
                     /locus_tag="U4A12_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN1773753.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="U4A12_001385"
     CDS             complement(14604..15446)
                     /locus_tag="U4A12_001385"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN1773754.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="U4A12_001386"
     CDS             complement(15439..16164)
                     /locus_tag="U4A12_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN1773755.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="U4A12_001387"
     CDS             16340..16636
                     /locus_tag="U4A12_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773756.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="U4A12_001388"
     CDS             17204..18796
                     /locus_tag="U4A12_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1773757.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="U4A12_001389"
     CDS             18809..19549
                     /locus_tag="U4A12_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1773758.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="U4A12_001390"
     CDS             19717..21066
                     /locus_tag="U4A12_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1773759.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="U4A12_001391"
     CDS             complement(21194..21784)
                     /locus_tag="U4A12_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1773760.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DASJOX010000041        21572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJOX010000041 DASJOX010000000
VERSION     DASJOX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.041"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            253..954
                     /locus_tag="U4A12_001392"
     CDS             253..954
                     /locus_tag="U4A12_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1773761.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1563..2579)
                     /gene="tsaD"
                     /locus_tag="U4A12_001393"
     CDS             complement(1563..2579)
                     /gene="tsaD"
                     /locus_tag="U4A12_001393"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN1773762.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2576..3040)
                     /gene="rimI"
                     /locus_tag="U4A12_001394"
     CDS             complement(2576..3040)
                     /gene="rimI"
                     /locus_tag="U4A12_001394"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1773763.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3047..3589)
                     /gene="rimI"
                     /locus_tag="U4A12_001395"
     CDS             complement(3047..3589)
                     /gene="rimI"
                     /locus_tag="U4A12_001395"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1773764.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3573..4295)
                     /gene="tsaB"
                     /locus_tag="U4A12_001396"
     CDS             complement(3573..4295)
                     /gene="tsaB"
                     /locus_tag="U4A12_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN1773765.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4486..5466)
                     /locus_tag="U4A12_001397"
     CDS             complement(4486..5466)
                     /locus_tag="U4A12_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773766.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5490..6974)
                     /gene="galT"
                     /locus_tag="U4A12_001398"
     CDS             complement(5490..6974)
                     /gene="galT"
                     /locus_tag="U4A12_001398"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN1773767.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6986..7975)
                     /gene="galE"
                     /locus_tag="U4A12_001399"
     CDS             complement(6986..7975)
                     /gene="galE"
                     /locus_tag="U4A12_001399"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN1773768.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8223..8390
                     /locus_tag="U4A12_001400"
     CDS             8223..8390
                     /locus_tag="U4A12_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN1773769.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8452..10263)
                     /locus_tag="U4A12_001401"
     CDS             complement(8452..10263)
                     /locus_tag="U4A12_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN1773770.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10260..10625)
                     /locus_tag="U4A12_001402"
     CDS             complement(10260..10625)
                     /locus_tag="U4A12_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN1773771.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10788..11183)
                     /locus_tag="U4A12_001403"
     CDS             complement(10788..11183)
                     /locus_tag="U4A12_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1773772.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11201..12163)
                     /locus_tag="U4A12_001404"
     CDS             complement(11201..12163)
                     /locus_tag="U4A12_001404"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN1773773.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12163..12375)
                     /locus_tag="U4A12_001405"
     CDS             complement(12163..12375)
                     /locus_tag="U4A12_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN1773774.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12396..13094)
                     /locus_tag="U4A12_001406"
     CDS             complement(12396..13094)
                     /locus_tag="U4A12_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1773775.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13114..13656)
                     /locus_tag="U4A12_001407"
     CDS             complement(13114..13656)
                     /locus_tag="U4A12_001407"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN1773776.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13788..14792)
                     /locus_tag="U4A12_001408"
     CDS             complement(13788..14792)
                     /locus_tag="U4A12_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1773777.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14789..15778)
                     /locus_tag="U4A12_001409"
     CDS             complement(14789..15778)
                     /locus_tag="U4A12_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1773778.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15775..16581)
                     /locus_tag="U4A12_001410"
     CDS             complement(15775..16581)
                     /locus_tag="U4A12_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773779.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16747..17703
                     /locus_tag="U4A12_001411"
     CDS             16747..17703
                     /locus_tag="U4A12_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1773780.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17780..18298)
                     /locus_tag="U4A12_001412"
     CDS             complement(17780..18298)
                     /locus_tag="U4A12_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1773781.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18386..18535)
                     /gene="rpmG"
                     /locus_tag="U4A12_001413"
     CDS             complement(18386..18535)
                     /gene="rpmG"
                     /locus_tag="U4A12_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1773782.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18763..19209
                     /locus_tag="U4A12_001414"
     CDS             18763..19209
                     /locus_tag="U4A12_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1773783.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19404..21299)
                     /gene="asnB"
                     /locus_tag="U4A12_001415"
     CDS             complement(19404..21299)
                     /gene="asnB"
                     /locus_tag="U4A12_001415"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN1773784.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6499 a         4564 c         3709 g         6800 t
ORIGIN      
        1 tttttgaata aattaaaaaa ataaattata ttatcgttgt tagcgtttac tatatgtatt
       61 attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat accattgtat
      121 attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc taatgttggt
      181 atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga acaataagga
      241 gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact cagatatcaa
      301 cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca tactgttctt
      361 cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca acggttgtat
      421 atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac ttattactac
      481 tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg agaagagacc
      541 tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc tgtttcctac
      601 tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca aaaaatttct
      661 aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag cgcctttgac
      721 cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac aaaagatggt
      781 catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag cggcacgaca
      841 cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt agattatgac
      901 aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga ataacgcgaa
      961 caaaaataag cagtgaccct gataaatatc agggtcactg cttattttcc tccctaaaac
     1021 ttctgaaaga tccggcagtt ttagatcact ggacaagact caaagatttt ctatgtgtgt
     1081 ttcagttatg taatattcac tgtttgcaaa aaatactgtt agattaacta gatgaagcgt
     1141 cttgtcctca tccgatctag attattcgat attcctcgac aataaacgat tactcagtga
     1201 gggcaatcag aggaacggat aatgataaat aagctgtaaa aaatagcaaa ataacaataa
     1261 tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg tgtcacagcc ctatttcgat
     1321 taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata aacatgagga
     1381 gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta cgaactcttg
     1441 attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt caaaaacatg
     1501 agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     1561 ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc aaaatttctt
     1621 ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc acaaagtcga
     1681 agagggggaa tcactagttc tacctcaggg agtttagctg ccaaagcatc ctgcaatcct
     1741 tcacgcaacc cttgattggc cgctacacct ccagctataa tcagttgctt gactggataa
     1801 ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc ttgaaagcta
     1861 gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg caccaaattg
     1921 ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg gatcatcgct
     1981 cgcggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc ttttccgctt
     2041 gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc tgcgtcatct
     2101 cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa ttctgtatgt
     2161 cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag acgggctgca
     2221 tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc ccaagcaaaa
     2281 gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc ataagtgaca
     2341 gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc ttccaagcat
     2401 aaggtgattt gttctacgtg atggcggcta gctacttcag gtactacgcc accaaaacgt
     2461 ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc atttctaacg
     2521 accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc ttcattcatt
     2581 tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc ggattgcgat
     2641 aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt tttcttgctg
     2701 cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt tgttccgtaa
     2761 tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa
     2821 tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca cagatctggt
     2881 tatcttcagc taaaagcaga taatcagagt gaggctgttg gatgtctgcc aaaaactgca
     2941 ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat acgcattctg
     3001 ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac tcattttcta
     3061 aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt tttttgatcg
     3121 ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc atcttgatta ctcatacgca
     3181 cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt tcgtcaataa
     3241 gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc gtgatatggc
     3301 aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta tcaaagatac
     3361 ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt tttgtccatg
     3421 gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata tcttctgtag
     3481 tcaattctct tagtacgtaa cgacgatcga tttgttcgac tgttttttct gtataaatca
     3541 ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata actttcgtct
     3601 ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta ctcgttttaa atagcgagga
     3661 agaaaagctt gtttctctgc aactggttca actaaagcac ctaattgagc cacagttaca
     3721 ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa tatttccttt
     3781 atttcagaga agaatttgtg acaatcactc ccgacgaaat aaatagcttc attcagcttt
     3841 tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac agtttctaat
     3901 ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc atcaaacatg
     3961 ggcacaataa tattatccac accgatgcaa ttagccgcga tggttttcag actagatact
     4021 cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac acctatacga
     4081 agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc ttttggactt
     4141 atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact gtggttgcgt
     4201 ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa
     4261 gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc tgctatgaca
     4321 ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc aaaggcaaaa
     4381 ctagtacaat aatacatgat tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca
     4441 aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt tcgttgattc
     4501 tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt tggaattact
     4561 taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa cttttacagt
     4621 agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca tcacagaaat
     4681 atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc
     4741 atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat attgcttcac
     4801 tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt cgtcgtagcc
     4861 tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc gctgatggtt
     4921 tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat gggagacaaa
     4981 gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt cgattccttg
     5041 agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca tttctaataa
     5101 ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat cactaatgga
     5161 atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt cgattcctag
     5221 gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc actggatcag
     5281 gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc
     5341 tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag gatcatagtt
     5401 gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt ctttaattgt
     5461 tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg ttcaaacgct
     5521 gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc
     5581 tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc aggatgttct
     5641 ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc ttctcctaat
     5701 aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac agcaagcccc
     5761 attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt atgtggatga
     5821 aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag gtccaactca
     5881 aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt accatctgct
     5941 gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc caatactgag
     6001 ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac agacatcggc
     6061 catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag tttttctatc
     6121 ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg agaaagaatc
     6181 gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg caagacttca
     6241 gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct
     6301 gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa tccccagctt
     6361 tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata gttcaagcga
     6421 cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac ttttaccgcc
     6481 tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt gattgtgatt
     6541 tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat ggctcttgtt
     6601 ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc ctctgggctt
     6661 ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt gagaaaatcc
     6721 attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac accattttct
     6781 cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac tggtcttact
     6841 tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg taaatataag
     6901 cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt
     6961 tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg ctgacatgcc
     7021 aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc cagcccagaa
     7081 tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct cttcttggtg
     7141 ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc atttccttga
     7201 ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct tctttcaagt
     7261 attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa tctctgatac
     7321 atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt ctttgaccaa
     7381 gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct tctccgatag
     7441 atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag cgcatgccat
     7501 atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca ccatacggat
     7561 ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct ccataagttg
     7621 ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact tcaagtaaga
     7681 tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca gattctccta
     7741 ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca aatacggaac
     7801 gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga tgtactgctt
     7861 gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt tgaactagct
     7921 ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt gtcataaaaa
     7981 acactccttt gattagtaaa attgtgtttg ttgattctat ataatttaaa aaacgaagtt
     8041 tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt aaatttataa
     8101 taaaacattt agtaaacatt ttcaagggtt ttttgcaaag gtttacaaac aaacgtaaaa
     8161 ctagcctttt tttctctttt gttccataat agatataaca aataataaat gaggtggatt
     8221 caatgaaaaa atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg
     8281 ctggtttggt tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa
     8341 tgatcgaaga aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa taattattta
     8401 aaaatacgca tgaagacttt ctaaaaaaca gataagtcat catgcgtatt tttatttgtc
     8461 agtcaatata agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg
     8521 tctagctact gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa
     8581 gtcagactgg atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat
     8641 agcttctgaa tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt
     8701 ttccttcaaa ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg
     8761 agcttcaatc aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt
     8821 gtgcttttgc tgaatatcaa atggcggttc gatcaaatga aaaatacccg catctatcag
     8881 tgctgctaat tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc tatttagatg
     8941 agtaaaccat ccccatagta tttctttgta ttccttagca gaaaaaacgt gccattcaat
     9001 agcctgccgg attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc
     9061 tgtgcaattg cctttttccg cttcttttat ctgcccttca ataaatattc ggctagcttc
     9121 tgaaaagggc tcattctgat aggcaagagg agtttctaaa tacgaccaat cccatctata
     9181 agggattagt gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg
     9241 ctcaaaattt ttctttggaa tcggcaagtg atattcttcg atgattttct tgtaataaaa
     9301 caactcaacg tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg
     9361 atgccattgc tccaggtttt cttttgttag taaccgtggc catgccattg ctccgccttg
     9421 cttttgattt cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat
     9481 cggttccttg cctgatggat gatagaccag ttttcccttt tcgttctcga agcgccctcc
     9541 tcttgatact gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa
     9601 gacattttct ttaggcggta tcttcgaaag atctacatct gcaggattag aaggaagctg
     9661 atacagtaaa acatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc
     9721 attttcccag tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt
     9781 ctgtaaaaga taaccatctt ttcttttgct tacttttttt actaaagaat aaacaaactg
     9841 aatagaagct ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta
     9901 taaacatcga gtagattgct gatctttttc cagacgattg atttcttcta taattagttg
     9961 cttatttttg gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa
    10021 caagttgggt ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt
    10081 cacttgttga gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg
    10141 acctccagga ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt
    10201 atggagaatc ctttctgcta cacttaatcc tctaggtccg gcaccaatga tccctatttt
    10261 catttgcatg cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca
    10321 ttttttgcac cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta
    10381 aaaatagctt gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat
    10441 gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt
    10501 tctttcaacc acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta
    10561 tccacgagta ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg
    10621 atcatagttg tttccttctt tctacctttt cgcttagctt atatatgtag ataatagcac
    10681 aaaataaaaa aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa
    10741 atctaatgat tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta
    10801 cttttgattt ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa
    10861 cctgctttac gtaatttatt tgctgtacgg atgctcaaac ttttcttttg atcgtaaata
    10921 tagacaggct ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg
    10981 tttcttgcac ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact
    11041 tgtgcttttc tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg
    11101 gccattactc gtagatagac ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg
    11161 atattaatta cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta
    11221 atgaggcagc accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg
    11281 attctcttac ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa
    11341 attctcctgc tgccgatact cctccaccta acacgatact tgacggattt aatgtattcc
    11401 ccaagtttcc gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa
    11461 acggatcgtc ttcttgagcc atttcaaaga tatctttact tgttacttct tctccgttgt
    11521 ctagcattgc tttcagtttt gaatcccctg cgtattcttc tgccaagaaa cgacctaggc
    11581 gtaccacacc tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg caagtacatt
    11641 cgaacccttc aggatccaca gtgatatgtc cgatttctcc tccagctccg ctcacgccgt
    11701 gaatcaaatt tccgccagca acgatacctc cgccaacacc tgttcctaga gtaacgaata
    11761 caacgtctgg atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg
    11821 cgtcattatc gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt
    11881 gaacttctgt ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc
    11941 ttcctggggt acccatgccg attccgataa agtcttcggc tttcatatta tataaattca
    12001 aacgatgatt gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat
    12061 tggtatcgat gctccatttt tgctgaatct ctccttctgg tgttaaaatt gcaaattttg
    12121 ctgttgttcc acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt
    12181 tcggctttct tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc
    12241 atgatcaatc aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc
    12301 atagatgcgt gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc
    12361 atataagctc tccatcttta agcacttcct tcatattata caagtaaacc atattttttt
    12421 aaacccagta aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt
    12481 tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat
    12541 ccgccaatta gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa
    12601 acaaatgtca acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta
    12661 tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactt
    12721 gcaccaccag acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc
    12781 ccgctgaaca tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc
    12841 cccataaaat acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag
    12901 attggtgtca ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg
    12961 gcataataag ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt
    13021 atccctaaaa aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc
    13081 tgttgataat tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt
    13141 ctgatcaaag ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca
    13201 tgttttttta tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg
    13261 ataaccggct ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa
    13321 atagagcttc tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg
    13381 ttcatccatt tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt
    13441 cttttgttct ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga
    13501 taaagtcgtc cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata
    13561 gattcgctgt tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat
    13621 tcctttccgg cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata
    13681 aaatggtata ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga
    13741 ataaaaagaa gacctagaaa tgattggcct tcctttttta atgaatttca actgttcagc
    13801 gcgtgttttt gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc
    13861 aaaattcctg taggtacttc cccacttggt agaatcactc tagcaaccat atctgcacta
    13921 agcagaaaaa tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt
    13981 acgactagct tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc
    14041 gctacactac cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc
    14101 acgttaaggc ctaatccttg cgcgcgttct tgaccaaatg atagcaatac aatcctctgt
    14161 gcataaagtc ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa
    14221 ggccagctcg caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc
    14281 ttagataatt ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata
    14341 ccggacaata agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag
    14401 acacagcaag cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc
    14461 gaatagtgcg aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg
    14521 cccaatatac tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa
    14581 acgccaagac aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta
    14641 acaatcagcg ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg
    14701 taaataaaag gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc
    14761 aagaaataga acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta
    14821 aaaacggaac gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga
    14881 tcatcctcgc tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag
    14941 gtagaacata acgatagtcc gttccaacca ttccgcgaac aactggcgga accaacagcc
    15001 caataaatga aacaggtccg actaacgaaa cggcaatggc tgctaacact gcaactagac
    15061 agaaaacaat caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac
    15121 tcaaatgaat caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat
    15181 ataaaggaaa caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc
    15241 aaaaagcaag atcctgctgc agattaaaga attgggtaag tgcttggctg atagcagtaa
    15301 aaaaagaact aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg
    15361 aaaactgcaa aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta
    15421 gaaacacaga aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg
    15481 aagcacccgc gttgattcct agtaagcctg aatctgctaa aggatttctt gtcacccctt
    15541 gcatcaatgc accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag
    15601 gcaaacgaat atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg
    15661 cctgccaaaa atctgctttt tccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa
    15721 taatcaaaag aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa
    15781 ttgatcctcc tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct
    15841 tggaagcgtg acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat
    15901 cacttcttca ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc
    15961 tgaaaaacgt gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat
    16021 ttgattgatt tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt
    16081 tgtcggttca tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac
    16141 acgctgggtt tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc
    16201 tgtagcatcc aacgcccact caattgcttc atgatcttgc gtgtccatgc cactgaacag
    16261 tttttgataa gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg
    16321 ttcactagtt tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga
    16381 gatatcagag ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact
    16441 tttcaataaa gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc
    16501 aggaatcgta acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc
    16561 agctgctctt aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga
    16621 cgctgtttca acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga
    16681 cagcgttcga aatatcagga ataataacca atgagaaaga ttctcaatta tgttaggagg
    16741 aaagaaatga aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga
    16801 gtactgagcg cttgctcccc ctcttcagat aaagaaacag ctacaactgc caattcgacg
    16861 acttcatcca atagtactca tacagtcaca gatacacttg gacataaagt agagattcca
    16921 aacgcgccca aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa
    16981 aaacctatcg cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat
    17041 ttaaaggatg tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgag
    17101 cctgatttgc tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat
    17161 agcaaaatcg cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa
    17221 ctaaaagatg tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat catttccgat
    17281 tatcaaaaat ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca
    17341 gctgcagtgt tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt
    17401 ggaagaatcg tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc
    17461 aaaaaagcca cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct
    17521 gactacatca tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg
    17581 aagaatctaa aagcagtgac agacaatcat gtactgcaat tcggacctga atctagttgg
    17641 ctatataacg gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat
    17701 tgattttaac taaaaaaagc atcttcacga ttagtcgtga gatgcttttt ttcttattaa
    17761 caaactagac gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc
    17821 atttttgaaa acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta
    17881 taagggcgaa gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat
    17941 cgatcaatgt tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac
    18001 cacccgaaac atagatatct tctgagatcg tttgactata cgacaaaagt tcatcgatcg
    18061 aatgcattac ttccgcattt tctttcgtgc gaaaatttgg ttgcgttgtc aacacgatga
    18121 tatggcgata aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta
    18181 aaattcgatc cattgtatgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta
    18241 acaaaccatt tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat
    18301 tatccgctcc ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag
    18361 caaggaattg agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt
    18421 cgcgtgggca atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg
    18481 ttagatagtt acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgtttt
    18541 tccctcccat ttttacaaga atcctctaag atgactctca gacttgatta tcttaccatg
    18601 attttttcta gattgctagt gtttttcacg attttgttaa gtaaaactac ttgacgataa
    18661 atacaacggt tttacttctc aaacaaaatc tttttctttt tgtataaata cccttacact
    18721 tgtttatata gattatgttt tttaataaag ggggatacta atatgccatg gaacatgaag
    18781 gactttcctg catcaatgaa aaacttagat aaattgacaa gaaaaaaagc aatcgacatc
    18841 gcaaatgcat tattagatga agggtaccct gataaccgtg cgatcccaat tgccattgat
    18901 caagcaaaaa aatggtatga agatgctagt gaatcagaaa aacgaacttt cgaaaaagaa
    18961 aaaaatccaa gcaagacgga tgaacacgat acgaatcctc gtgctggaaa actgcttgat
    19021 tcagatgtga tcgtagaata tgaggaagaa caatggatcg tgaaatctaa aggggcaaaa
    19081 aaggcaagca accattttga tacaaaaaaa gaagcaatcg aaaaaggaaa gcaagtagca
    19141 caaaacaaag aatcaacttt gttcatttat aaaaaagatg ggacaaaaga aaaagaaatc
    19201 gattattgaa catatgtaaa ggggttgtga cgaaaatcgt gtcacaaccc ccttatgttc
    19261 gaaacaatgg tgttcctctg ccaactccga accataagtc ataatatcta taaaacggga
    19321 agagctgttt tttgaaattg tttcttatca ctcagtgtta aacggtagga gaaaacctct
    19381 tttttattga tatttgattt tcatcaaaca gtcacatagt cgattgcttc tgcttgcgga
    19441 atcgtttcat ctacaaagta gactttatac catgtcaaga aactgaagat tgtccagatt
    19501 tttctttgct cttctttacg tccttcgtga tggtcatcta gcaacttcat gattttttct
    19561 tgatcaaaaa attcttttgc aaaatctgct tcgaataatg ctttgacttg ttcgtagccg
    19621 tgttcttctt tcaaccaagc tttgattggc acagggaatc ctaatttcac acgattcgac
    19681 cattcttccg gaagatgttt gttcgcagct tgtctgaaaa tatcttttgt gttgttttga
    19741 ttcaataaaa acttacttgg tatttgttga gccaatttca tgacttcgat atccaataaa
    19801 ggtactcgga cttccaaaga acttgccata gaaagtttat ctgcctttaa caagatatct
    19861 tttggcatcc attgatgaag atcgacatat tgcatcttat tgacttcatc tttgattcct
    19921 tctgttttct cgtaatgaac agtcatgatt tcatccacag atggtgctgt ttgatatttt
    19981 ggtgttaaca cttctgatgc ttctgattct tcgaacactc gagcatgacc gataaaatat
    20041 tcttttgcag gagcaagtga ttcatataaa tggatccgtc catggaagtt tttcattttt
    20101 ccgattttac gtcctaaatt atagcgtgtt ccttttggta attttttcaa tccttgagca
    20161 atcacacgga tgaattttga attcgtatgg aatccatacg tttggtatcc cgcaaacagt
    20221 tcatccgccc cttctccaga ttggactacc cgcacacttt gtgaagcaag atttgctaaa
    20281 aagtacaaag gtacgcaaga aggatttgaa tcaggttcat ctaagtgata ctggatcaaa
    20341 gggaatgctt tgaacgacat gtctccgtcg atcactgctg ctgtattatc taagtccaat
    20401 agttcggtta gttttctggc ttcaatcgct tcattatagg ttttatcatc aaacccgatt
    20461 gagaaagaat gatctggttt caatacagag gttacataac tggaatcaac cccgctggat
    20521 aagaaagaac caacttctac atcactgacc gtatgtgctt caatagaagc ttgaacggtt
    20581 tcgtcgattt tttcgatcca ttcttgtcgg gaatgcgttt cttttgtttc aaaatctgca
    20641 tcccaatatt gctgaatgtc tatttttcca tctttatacg tataataatg gccttcaggc
    20701 aaacgataga catctttgaa gaatgtttcg ccgtttaatg gagaatattg gaaagtcata
    20761 taaggtttca acgcttcttt gttcaattct ttgttaaaat caggatgagg cagaaaactt
    20821 ttgatttctg aaccaaacat aaatgttccg ttcatttctg cataataata tggtttgatc
    20881 ccaaagtggt ctcttgcacc gaataactca ttcttttcat tatcccaaat agcaaaagca
    20941 aacattccgc ggatcttttg caataattct gttccccatt cttcgtaacc atgcaaaagg
    21001 acttctgtat ctgcatgtgt ttgaaaaacg tggccagctg caatcaattc ttctctcaaa
    21061 ggctggtaat tatagatttc gccattaaaa ataatgatct ttgtccgatc ttcattgtaa
    21121 ataggctgag taccgccctc taagtcaata atacttaatc ttctgaatcc taaagcaaca
    21181 tgtttatcga tatattctcc tgaactatta ggtccacggt gaacgattcg atccatcatt
    21241 gtattaataa ttgttttttt gttgtccttg tcgttaacaa aacctacaat accgcacatt
    21301 taactttcac atcctaatat aaaattactg tttatttcat tcatatgcat cttatcatag
    21361 aataaatagc ggacgaatat tttctctctt acaatttcgt cacgtgaaaa caaaagatga
    21421 aagtttctgt ttttacagaa aataaagtcc caatatctaa tttaccatgt tttctttttt
    21481 gaggctacct tattctcttc gtgttaaaaa aatattagtg gatgctattt ccaaaaaaac
    21541 cagcaaaaaa agacacgacc taaagatcgt gt
//
LOCUS       DASJOX010000042        21284 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJOX010000042 DASJOX010000000
VERSION     DASJOX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.042"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..2389)
                     /locus_tag="U4A12_001416"
     CDS             complement(8..2389)
                     /locus_tag="U4A12_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1773785.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="U4A12_001417"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="U4A12_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN1773786.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="U4A12_001418"
     CDS             3090..3629
                     /locus_tag="U4A12_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN1773787.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="U4A12_001419"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="U4A12_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN1773788.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="U4A12_001420"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4A12_001420"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="U4A12_001421"
     CDS             4736..5902
                     /locus_tag="U4A12_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN1773789.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="U4A12_001422"
     CDS             5948..7444
                     /locus_tag="U4A12_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN1773790.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="U4A12_001423"
     CDS             7525..8208
                     /locus_tag="U4A12_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1773791.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="U4A12_001424"
     CDS             8853..10043
                     /locus_tag="U4A12_001424"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN1773792.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="U4A12_001425"
     CDS             10145..11098
                     /locus_tag="U4A12_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN1773793.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="U4A12_001426"
     CDS             11167..12516
                     /locus_tag="U4A12_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1773794.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="U4A12_001427"
     CDS             complement(12699..13022)
                     /locus_tag="U4A12_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773795.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="U4A12_001428"
     CDS             13151..14314
                     /locus_tag="U4A12_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1773796.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="U4A12_001429"
     CDS             14384..15733
                     /locus_tag="U4A12_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1773797.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="U4A12_001430"
     CDS             complement(15778..16209)
                     /locus_tag="U4A12_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773798.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="U4A12_001431"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="U4A12_001431"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN1773799.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="U4A12_001432"
     CDS             19439..20035
                     /locus_tag="U4A12_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1773800.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..>21284
                     /locus_tag="U4A12_001433"
     CDS             20157..>21284
                     /locus_tag="U4A12_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1773801.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFD"
BASE COUNT         6866 a         3767 c         4511 g         6140 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgat
//
LOCUS       DASJOX010000043        21234 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJOX010000043 DASJOX010000000
VERSION     DASJOX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.043"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..687)
                     /locus_tag="U4A12_001434"
     CDS             complement(40..687)
                     /locus_tag="U4A12_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773802.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(866..1585)
                     /locus_tag="U4A12_001435"
     CDS             complement(866..1585)
                     /locus_tag="U4A12_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1773803.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1716..2867)
                     /locus_tag="U4A12_001436"
     CDS             complement(1716..2867)
                     /locus_tag="U4A12_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN1773804.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3084..3824)
                     /locus_tag="U4A12_001437"
     CDS             complement(3084..3824)
                     /locus_tag="U4A12_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1773805.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3828..4151)
                     /gene="rsfS"
                     /locus_tag="U4A12_001438"
     CDS             complement(3828..4151)
                     /gene="rsfS"
                     /locus_tag="U4A12_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN1773806.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4208..4801)
                     /gene="yqeK"
                     /locus_tag="U4A12_001439"
     CDS             complement(4208..4801)
                     /gene="yqeK"
                     /locus_tag="U4A12_001439"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN1773807.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4785..5435)
                     /locus_tag="U4A12_001440"
     CDS             complement(4785..5435)
                     /locus_tag="U4A12_001440"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN1773808.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5445..5756)
                     /gene="yhbY"
                     /locus_tag="U4A12_001441"
     CDS             complement(5445..5756)
                     /gene="yhbY"
                     /locus_tag="U4A12_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN1773809.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5772..6881)
                     /gene="yqeH"
                     /locus_tag="U4A12_001442"
     CDS             complement(5772..6881)
                     /gene="yqeH"
                     /locus_tag="U4A12_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN1773810.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6878..7405)
                     /locus_tag="U4A12_001443"
     CDS             complement(6878..7405)
                     /locus_tag="U4A12_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN1773811.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7585..8370)
                     /locus_tag="U4A12_001444"
     CDS             complement(7585..8370)
                     /locus_tag="U4A12_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN1773812.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8363..9232)
                     /locus_tag="U4A12_001445"
     CDS             complement(8363..9232)
                     /locus_tag="U4A12_001445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN1773813.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9233..10612)
                     /locus_tag="U4A12_001446"
     CDS             complement(9233..10612)
                     /locus_tag="U4A12_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN1773814.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10620..11039)
                     /gene="fabZ"
                     /locus_tag="U4A12_001447"
     CDS             complement(10620..11039)
                     /gene="fabZ"
                     /locus_tag="U4A12_001447"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1773815.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11064..11540)
                     /gene="accB"
                     /locus_tag="U4A12_001448"
     CDS             complement(11064..11540)
                     /gene="accB"
                     /locus_tag="U4A12_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN1773816.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11543..12778)
                     /gene="fabF"
                     /locus_tag="U4A12_001449"
     CDS             complement(11543..12778)
                     /gene="fabF"
                     /locus_tag="U4A12_001449"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN1773817.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12797..13534)
                     /gene="fabG"
                     /locus_tag="U4A12_001450"
     CDS             complement(12797..13534)
                     /gene="fabG"
                     /locus_tag="U4A12_001450"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN1773818.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13546..14463)
                     /gene="fabD"
                     /locus_tag="U4A12_001451"
     CDS             complement(13546..14463)
                     /gene="fabD"
                     /locus_tag="U4A12_001451"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1773819.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14570..14794)
                     /locus_tag="U4A12_001452"
     CDS             complement(14570..14794)
                     /locus_tag="U4A12_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1773820.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14847..15809)
                     /locus_tag="U4A12_001453"
     CDS             complement(14847..15809)
                     /locus_tag="U4A12_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN1773821.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15819..16259)
                     /locus_tag="U4A12_001454"
     CDS             complement(15819..16259)
                     /locus_tag="U4A12_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1773822.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16565..17530
                     /locus_tag="U4A12_001455"
     CDS             16565..17530
                     /locus_tag="U4A12_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1773823.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17587..17817)
                     /locus_tag="U4A12_001456"
     CDS             complement(17587..17817)
                     /locus_tag="U4A12_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN1773824.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17903..18790)
                     /locus_tag="U4A12_001457"
     CDS             complement(17903..18790)
                     /locus_tag="U4A12_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1773825.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18879..20102)
                     /locus_tag="U4A12_001458"
     CDS             complement(18879..20102)
                     /locus_tag="U4A12_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1773826.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20119..21165)
                     /locus_tag="U4A12_001459"
     CDS             complement(20119..21165)
                     /locus_tag="U4A12_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1773827.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         5755 a         4707 c         3594 g         7178 t
ORIGIN      
        1 aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat ataaatccaa
       61 atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt cttccatgtt
      121 ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat tgtcgatcaa
      181 tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc tgattggata
      241 gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat cgatcacttt
      301 attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag gacgatgttt
      361 caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt caaataaccg
      421 aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc tataaatcag
      481 cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg taccgacata
      541 gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta attttgaaca
      601 atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga tacgttcaaa
      661 aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac atacttcata
      721 tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa aagaaaataa
      781 atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg aaaaagactg
      841 aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg aacacatgct
      901 gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct tgatcttctg
      961 gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa aaatcagtga
     1021 ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca ccgtaaacga
     1081 tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc attaggtatt
     1141 caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt
     1201 aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct gcccgtacgt
     1261 ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt gagccgcttg
     1321 gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct tttgcttttt
     1381 cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga tcaatgacga
     1441 aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc gcgtagattt
     1501 caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt ttctttgcta
     1561 cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga tagtttcata
     1621 acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag ctttttttct
     1681 ttcttccttc attcttctca tttttagtca attatctata ctcggattgg gatttgcccg
     1741 aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct aactaacagt
     1801 tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc ttttttttct
     1861 tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt tttattttgt
     1921 tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa tcgttggatc
     1981 cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa actatcagca
     2041 gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata gatatctgtc
     2101 agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata agattcccaa
     2161 gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag tccagctatt
     2221 ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct ctgatctgtg
     2281 atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc catagggtgg
     2341 acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg cgaagaaaaa
     2401 tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg cggataacta
     2461 tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca
     2521 aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa acacaaactg
     2581 tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc agctgactga
     2641 accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa agcttcattc
     2701 gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt
     2761 acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg atagcgatgt
     2821 ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg catcctcctt
     2881 catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat
     2941 acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg
     3001 caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt
     3061 gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat
     3121 ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg
     3181 ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct
     3241 tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg
     3301 atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga
     3361 taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg tacgtcaaat
     3421 aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct
     3481 tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg
     3541 atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat
     3601 gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat
     3661 ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc caaaatattt
     3721 tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata aagtgtttcg
     3781 tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat
     3841 tcactaagat cgactagcgg agcatctgac catagttttt ccagattata gaaatttcgt
     3901 tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg
     3961 ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt
     4021 tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct
     4081 gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg ttttgaatct
     4141 gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat
     4201 gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct
     4261 gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt
     4321 ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt
     4381 caatatagtc tgctacataa atgattttgt caaggagact catcttagca gccccagtcg
     4441 tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc cgctctacaa
     4501 acgaagcgcc aacaagacca tgccagattg cattattata attcaataac tccggatcaa
     4561 aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat
     4621 gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca
     4681 cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta
     4741 ttttttgcat caggttttct ctagaataag cagtatattc attactatag tccatattca
     4801 tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa
     4861 cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct
     4921 acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag
     4981 ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc
     5041 ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta
     5101 tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga
     5161 ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc
     5221 tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc
     5281 cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc
     5341 aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt
     5401 gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact
     5461 tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt
     5521 ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct
     5581 gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct
     5641 tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc
     5701 tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt
     5761 ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag
     5821 cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct
     5881 gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca
     5941 ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc
     6001 agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga agcacgattg
     6061 ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc
     6121 tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct
     6181 aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg
     6241 tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt
     6301 acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca
     6361 tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat
     6421 agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt
     6481 cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta
     6541 ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt
     6601 agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca
     6661 tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct
     6721 tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct
     6781 aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt
     6841 ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc
     6901 ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa
     6961 attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg
     7021 cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat
     7081 ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc
     7141 gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt
     7201 ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact
     7261 cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg
     7321 ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc
     7381 atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca
     7441 taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact
     7501 cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca
     7561 gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc gtttttccag
     7621 taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat
     7681 aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg
     7741 atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc
     7801 tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc
     7861 ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc
     7921 acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag
     7981 cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc
     8041 acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt gtttcatcag
     8101 tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc
     8161 ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc
     8221 gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc attcctcgaa
     8281 agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa
     8341 ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt
     8401 caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac
     8461 catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct
     8521 gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaataatat
     8581 cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta
     8641 gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca
     8701 ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg
     8761 gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc
     8821 ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga atcccctgaa
     8881 tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa
     8941 ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt
     9001 cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag
     9061 cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt
     9121 tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc
     9181 tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc
     9241 ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc
     9301 tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat tctgcgttcg
     9361 tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa
     9421 aacgcgtatt tccatggacg attatttttg caatcattga atcataatat ggcgggatcg
     9481 tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct ggaagcagta
     9541 agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc
     9601 ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac
     9661 cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg
     9721 gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca
     9781 tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag
     9841 cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta
     9901 ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa
     9961 atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat
    10021 acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg
    10081 ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt
    10141 tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata
    10201 atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat
    10261 tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc
    10321 actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa
    10381 ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat
    10441 caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt
    10501 cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa
    10561 tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc
    10621 tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt
    10681 cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta
    10741 tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc
    10801 tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc cattgcttca
    10861 acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca
    10921 ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga
    10981 tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt
    11041 gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat
    11101 tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt
    11161 atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga
    11221 tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac gattcccacg
    11281 attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt cattatttct
    11341 ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg caacgtttcg
    11401 tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat
    11461 tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct
    11521 ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat
    11581 gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt
    11641 ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc gtaggtggga
    11701 taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca
    11761 ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta
    11821 atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag
    11881 cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg
    11941 ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg
    12001 caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag
    12061 attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga ttttcgtcaa
    12121 acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc
    12181 cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat
    12241 agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg
    12301 ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt ccagctgcca
    12361 tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta
    12421 gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc
    12481 caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag
    12541 cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat
    12601 atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat
    12661 caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt
    12721 ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact cgattcattt
    12781 gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc
    12841 cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc
    12901 cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt
    12961 ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg
    13021 cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat
    13081 ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg atggcccctt
    13141 ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat
    13201 tgatatccag acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg
    13261 tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga
    13321 tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac
    13381 cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga
    13441 tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac
    13501 ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat
    13561 tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt
    13621 tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat
    13681 tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag
    13741 tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa
    13801 agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc
    13861 taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt
    13921 ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg
    13981 ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct
    14041 ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg
    14101 ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga
    14161 tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc
    14221 tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc
    14281 tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat
    14341 atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata
    14401 taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt
    14461 catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga
    14521 gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt
    14581 gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt
    14641 tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt
    14701 ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt
    14761 ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt
    14821 gctttttatt tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa
    14881 cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat ctttccacta
    14941 gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc
    15001 atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt
    15061 ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg
    15121 atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa
    15181 taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg
    15241 gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt
    15301 ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga
    15361 tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc cttagttgag
    15421 ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca
    15481 ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca
    15541 gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg
    15601 tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc
    15661 tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag
    15721 gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct
    15781 gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag
    15841 tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct
    15901 aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct
    15961 tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca
    16021 tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt
    16081 tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc
    16141 atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc
    16201 aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc tggttccatg
    16261 ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa
    16321 cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat
    16381 tttactcatt taaaacaact gataaatcag catttataat ttcttttatt agaaaaatca
    16441 tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat agaattccct
    16501 aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga
    16561 tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa
    16621 agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag
    16681 cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat
    16741 aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca
    16801 attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca gtaattataa
    16861 ttatgtacat attaccaata agggatttgt attaacagat actcctacgg ctcgaacata
    16921 cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg aagaaactta
    16981 tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc ttcctgacct
    17041 acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa
    17101 taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc agttgaattt
    17161 cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta ttagacggat
    17221 ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc cattagcagc
    17281 agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag aaacaatgat
    17341 tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc atttatcttt
    17401 gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca gttaccgcaa
    17461 aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag ctgaaaagca
    17521 actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg tcaattatcc
    17581 taaaatttat tggattaagt catagatttc catcgctacg atatcgatat tatcaaattc
    17641 gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt tatcataagt
    17701 tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt cattatctgc
    17761 tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat gtgtcatcgg
    17821 aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac ttgcattgat
    17881 tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct tcaggccacc
    17941 aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc aactcttcat
    18001 atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct gcttcttcta
    18061 atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt
    18121 ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca cctgaaagcg
    18181 tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc atttccgtca
    18241 ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc tgacctttcc
    18301 cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc agtacttgat
    18361 cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt gctcctaagt
    18421 caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa
    18481 ttttttctgc caggcttggt gtacttgtag tcaaatcaac taaaatatgt cctgagatat
    18541 ctgcagaaaa aatccccgac tgtccatagt agatttcttc tacatctttg gggtaaccta
    18601 ccattgtaaa gagaatatta ctttgttctg ctaccgcttt tggtgtatct gcccagatag
    18661 ctccttctgt tactaaagga tcagcttttg cttttgtccg attatagaca gctacttcgt
    18721 gtcctgcttc tagtagatgg cgtgcgacag cacttcccat gacgcctgtt ccgataaaac
    18781 caagtttcat tttttcgacc tcctaaaaat caaaaatcct tgcttctatg ctagtatacg
    18841 ctagaaacaa ggattttttc ataaaaaatg tttgaatttt atcttttaaa tgaatttaat
    18901 tttattttga ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc ttcttcatga
    18961 taaacaacca tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt agatgaaata
    19021 tcataaagtt tagcttctaa aacttttgga tctttaggaa cttcactgga aatataatca
    19081 ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg
    19141 aacaatcggt tcaaataagc atttccttcc acgacacaag gtgttccggc tgccatcgct
    19201 tctgtataag taagcccttg tgtttctgaa gtagaagcac tgacaaaata atctgccgct
    19261 ttataataaa gtgctacatc attgttgtcc acttctcctg tgaactgcac aaattcttct
    19321 aactgcagtt ctgagatcaa ttcctctaac tcttctacat aaggtccttt tcctacgata
    19381 actaatctgg cgttaggata agcggagatg acctgaggaa acccattaat caaggcttgg
    19441 atatttttct cataagaaat cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga
    19501 cctagtgact cccttaattt gctgatatcc tgctgagtga tatcagaacg tttgaatttt
    19561 tcaatgtcga taccagtagg aatcacgcga agcggtgcta ttacaccata gctgcgtaat
    19621 gtatcaataa cacgttcact tggacaaact acacctgtcg tatgatttgc aaataatcta
    19681 gacaagtatt ttacatggga ttgcctgatc acttttcctt taccgatata atgaaggtag
    19741 tcttcataca tcgtatggta agtatggatc agaggtattt tcaacttctt agctaccatc
    19801 ttcccaagta tccctgctcc gaattctgta tgggtatgaa taagatctag ttctagttct
    19861 ttggcaacca aataagcata ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa
    19921 ataaatggta cacttggcat tcgaatcaca tctttttcca acttatccgc attaggatcg
    19981 gtcgttgtaa aaatatagac ttcatgccca tgtctttcta attcttgttt taatgttttg
    20041 atcgatgtag caactccgct gacttgcgga aagtaggtat cggtaaaaaa gccgattttc
    20101 atgtttttcg cctccaaact agtccttgac ctgatttact ttttttaatt tgctagagac
    20161 tttttcattc agtacacttt cgtatacatt tttaagttct tcaccgattt tattgatact
    20221 tcgctctttt gctgtttgat aaccggcttg tgtcgtactc ggtagttctt gattggtaat
    20281 ctgttggatc agtcggataa attcttcgtt tgtcttcccc atatagcagt tcttgctatc
    20341 ttctaaccag ccattataca ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa
    20401 agcttctaaa acaacgatcc cttctgtctc ttcataagaa gggaaaaaga atagatctgc
    20461 acctgaatac gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca ggacattttc
    20521 aggatgatct tcttttacaa ttttccggat gttccgagga atcgagtaca tgggaacatg
    20581 accaaaccaa ataaatgtgt attcgggcat cttcttagca atttctacga aatcaatgat
    20641 tcctttacgt tcgaaaaaca aaccgacaca aatgattact ttttgatccg gagacagttt
    20701 gaaatattct ctgaactttt gttctttcat tggatcaggg tgatatttct ctaagtcgat
    20761 cccattggaa atagcttgaa tcggcacacg gataccatag ctttccaaca actgttttga
    20821 ataaggtgtt ggcgtgatca gatagtccgc tttttggtac aatcctacca gatatttttt
    20881 tacgatagga gaaagctggt tcgatccgat aaaggaattt cggaaatctt cctctgtaga
    20941 atgcgcatga tagacaactt tttttccttc tcttctggct ttattgacca tattatgact
    21001 attgattccg tatgtattga tgtgcaggat gtcataatct tctttttcat ctagtgtata
    21061 agagataccg acttccgtca gagcacgttt ttgatgatca agcgcacgcc cgattccaga
    21121 ttttgcaagc atcttttcac tttcaaaata taataatacc ttcaccttac gtcccccttc
    21181 acttatatag tataacatag cctacaaact gtcactcaaa cttaagccct attt
//
LOCUS       DASJOX010000044        19126 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJOX010000044 DASJOX010000000
VERSION     DASJOX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.045"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..703)
                     /locus_tag="U4A12_001460"
     CDS             complement(<1..703)
                     /locus_tag="U4A12_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1773828.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="U4A12_001461"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="U4A12_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="U4A12_001462"
     CDS             1758..2837
                     /locus_tag="U4A12_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1773829.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="U4A12_001463"
     CDS             2869..3462
                     /locus_tag="U4A12_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773830.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="U4A12_001464"
     CDS             complement(3572..3799)
                     /locus_tag="U4A12_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1773831.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="U4A12_001465"
     CDS             complement(3902..6490)
                     /locus_tag="U4A12_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN1773832.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="U4A12_001466"
     CDS             complement(6510..7169)
                     /locus_tag="U4A12_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1773833.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="U4A12_001467"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="U4A12_001467"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN1773834.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="U4A12_001468"
     CDS             complement(7942..8808)
                     /locus_tag="U4A12_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1773835.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="U4A12_001469"
     CDS             8917..9558
                     /locus_tag="U4A12_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN1773836.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="U4A12_001470"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="U4A12_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN1773837.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="U4A12_001471"
     CDS             complement(10999..11898)
                     /locus_tag="U4A12_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1773838.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="U4A12_001472"
     CDS             complement(11900..12697)
                     /locus_tag="U4A12_001472"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN1773839.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="U4A12_001473"
     CDS             complement(12675..13379)
                     /locus_tag="U4A12_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN1773840.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="U4A12_001474"
     CDS             13510..14085
                     /locus_tag="U4A12_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEN1773841.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="U4A12_001475"
     CDS             14203..14880
                     /locus_tag="U4A12_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1773842.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="U4A12_001476"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="U4A12_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN1773843.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="U4A12_001477"
     CDS             complement(16943..17941)
                     /locus_tag="U4A12_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN1773844.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="U4A12_001478"
     CDS             complement(18167..18820)
                     /locus_tag="U4A12_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN1773845.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DASJOX010000045        18376 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJOX010000045 DASJOX010000000
VERSION     DASJOX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.046"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="U4A12_001479"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="U4A12_001479"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN1773846.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="U4A12_001480"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="U4A12_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN1773847.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="U4A12_001481"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="U4A12_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN1773848.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="U4A12_001482"
     CDS             2857..3732
                     /locus_tag="U4A12_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1773849.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="U4A12_001483"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="U4A12_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN1773850.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="U4A12_001484"
     CDS             4629..5063
                     /locus_tag="U4A12_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN1773851.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="U4A12_001485"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="U4A12_001485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN1773852.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="U4A12_001486"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="U4A12_001486"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN1773853.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="U4A12_001487"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="U4A12_001487"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN1773854.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="U4A12_001488"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="U4A12_001488"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN1773855.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="U4A12_001489"
     CDS             13334..14260
                     /locus_tag="U4A12_001489"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN1773856.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="U4A12_001490"
     CDS             14442..15335
                     /locus_tag="U4A12_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN1773857.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="U4A12_001491"
     CDS             15469..15594
                     /locus_tag="U4A12_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773858.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="U4A12_001492"
     CDS             complement(15728..16360)
                     /locus_tag="U4A12_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773859.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="U4A12_001493"
     CDS             complement(16508..17050)
                     /locus_tag="U4A12_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN1773860.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="U4A12_001494"
     CDS             17185..17574
                     /locus_tag="U4A12_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773861.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="U4A12_001495"
     CDS             17578..18342
                     /locus_tag="U4A12_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1773862.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
BASE COUNT         6453 a         3136 c         3878 g         4909 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggat
//
LOCUS       DASJOX010000046        18195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJOX010000046 DASJOX010000000
VERSION     DASJOX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.047"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..152
                     /locus_tag="U4A12_001496"
     CDS             <1..152
                     /locus_tag="U4A12_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1773863.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="U4A12_001497"
     CDS             complement(230..763)
                     /locus_tag="U4A12_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN1773864.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="U4A12_001498"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="U4A12_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN1773865.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="U4A12_001499"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="U4A12_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN1773866.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="U4A12_001500"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="U4A12_001500"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1773867.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="U4A12_001501"
     CDS             complement(4488..5066)
                     /locus_tag="U4A12_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN1773868.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="U4A12_001502"
     CDS             5388..5858
                     /locus_tag="U4A12_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN1773869.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="U4A12_001503"
     CDS             complement(5966..7192)
                     /locus_tag="U4A12_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1773870.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="U4A12_001504"
     CDS             complement(7946..8146)
                     /locus_tag="U4A12_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1773871.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="U4A12_001505"
     CDS             complement(8280..8888)
                     /locus_tag="U4A12_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN1773872.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="U4A12_001506"
     CDS             complement(8973..9443)
                     /locus_tag="U4A12_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN1773873.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="U4A12_001507"
     CDS             complement(9491..10405)
                     /locus_tag="U4A12_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1773874.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="U4A12_001508"
     CDS             10551..11354
                     /locus_tag="U4A12_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN1773875.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="U4A12_001509"
     CDS             11532..12479
                     /locus_tag="U4A12_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN1773876.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="U4A12_001510"
     CDS             complement(12547..12840)
                     /locus_tag="U4A12_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN1773877.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="U4A12_001511"
     CDS             complement(12868..13533)
                     /locus_tag="U4A12_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN1773878.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="U4A12_001512"
     CDS             complement(13673..14305)
                     /locus_tag="U4A12_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773879.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="U4A12_001513"
     CDS             complement(14312..15379)
                     /locus_tag="U4A12_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1773880.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="U4A12_001514"
     CDS             complement(15376..16107)
                     /locus_tag="U4A12_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN1773881.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="U4A12_001515"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="U4A12_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN1773882.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="U4A12_001516"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="U4A12_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN1773883.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5405 a         3687 c         2908 g         6195 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaa
//
LOCUS       DASJOX010000047        17977 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJOX010000047 DASJOX010000000
VERSION     DASJOX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.048"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..727)
                     /locus_tag="U4A12_001517"
     CDS             complement(29..727)
                     /locus_tag="U4A12_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN1773884.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="U4A12_001518"
     CDS             complement(720..2072)
                     /locus_tag="U4A12_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN1773885.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="U4A12_001519"
     CDS             complement(2295..3695)
                     /locus_tag="U4A12_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN1773886.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="U4A12_001520"
     CDS             3856..4593
                     /locus_tag="U4A12_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN1773887.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="U4A12_001521"
     CDS             4718..5197
                     /locus_tag="U4A12_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN1773888.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="U4A12_001522"
     CDS             5194..5961
                     /locus_tag="U4A12_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773889.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="U4A12_001523"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="U4A12_001523"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1773890.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="U4A12_001524"
     CDS             complement(6864..7466)
                     /locus_tag="U4A12_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1773891.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="U4A12_001525"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="U4A12_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="U4A12_001526"
     CDS             8147..8731
                     /locus_tag="U4A12_001526"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1773892.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="U4A12_001527"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="U4A12_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN1773893.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="U4A12_001528"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="U4A12_001528"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN1773894.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="U4A12_001529"
     CDS             10632..10811
                     /locus_tag="U4A12_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773895.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="U4A12_001530"
     CDS             10833..11858
                     /locus_tag="U4A12_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN1773896.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="U4A12_001531"
     CDS             12033..13277
                     /locus_tag="U4A12_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN1773897.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="U4A12_001532"
     CDS             13430..14377
                     /locus_tag="U4A12_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1773898.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="U4A12_001533"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="U4A12_001533"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN1773899.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="U4A12_001534"
     CDS             complement(15279..15521)
                     /locus_tag="U4A12_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773900.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="U4A12_001535"
     tRNA            15642..15714
                     /locus_tag="U4A12_001535"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="U4A12_001536"
     CDS             complement(15833..16471)
                     /locus_tag="U4A12_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1773901.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="U4A12_001537"
     CDS             complement(16594..17226)
                     /locus_tag="U4A12_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1773902.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="U4A12_001538"
     CDS             17577..17876
                     /locus_tag="U4A12_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1773903.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6154 a         3150 c         3554 g         5119 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaaat
//
LOCUS       DASJOX010000048        17171 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJOX010000048 DASJOX010000000
VERSION     DASJOX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.049"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            30..290
                     /locus_tag="U4A12_001539"
     CDS             30..290
                     /locus_tag="U4A12_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773904.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(652..891)
                     /locus_tag="U4A12_001540"
     CDS             complement(652..891)
                     /locus_tag="U4A12_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773905.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1177..1491
                     /locus_tag="U4A12_001541"
     CDS             1177..1491
                     /locus_tag="U4A12_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773906.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1925..2291)
                     /gene="ssrA"
                     /locus_tag="U4A12_001542"
     tmRNA           complement(1925..2291)
                     /gene="ssrA"
                     /locus_tag="U4A12_001542"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2462..3235)
                     /locus_tag="U4A12_001543"
     CDS             complement(2462..3235)
                     /locus_tag="U4A12_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1773907.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3379..3729
                     /locus_tag="U4A12_001544"
     CDS             3379..3729
                     /locus_tag="U4A12_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1773908.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3710..4426
                     /locus_tag="U4A12_001545"
     CDS             3710..4426
                     /locus_tag="U4A12_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1773909.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4426..5874
                     /locus_tag="U4A12_001546"
     CDS             4426..5874
                     /locus_tag="U4A12_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1773910.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5944..6453)
                     /locus_tag="U4A12_001547"
     CDS             complement(5944..6453)
                     /locus_tag="U4A12_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773911.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6443..7168)
                     /locus_tag="U4A12_001548"
     CDS             complement(6443..7168)
                     /locus_tag="U4A12_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773912.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7391..9511)
                     /locus_tag="U4A12_001549"
     CDS             complement(7391..9511)
                     /locus_tag="U4A12_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1773913.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9741..10919
                     /locus_tag="U4A12_001550"
     CDS             9741..10919
                     /locus_tag="U4A12_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN1773914.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10935..11693
                     /gene="aroD"
                     /locus_tag="U4A12_001551"
     CDS             10935..11693
                     /gene="aroD"
                     /locus_tag="U4A12_001551"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN1773915.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11716..12201
                     /gene="ybaK"
                     /locus_tag="U4A12_001552"
     CDS             11716..12201
                     /gene="ybaK"
                     /locus_tag="U4A12_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN1773916.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12272..13525)
                     /locus_tag="U4A12_001553"
     CDS             complement(12272..13525)
                     /locus_tag="U4A12_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1773917.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13643..14992
                     /gene="gdhA"
                     /locus_tag="U4A12_001554"
     CDS             13643..14992
                     /gene="gdhA"
                     /locus_tag="U4A12_001554"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN1773918.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15104..16450
                     /locus_tag="U4A12_001555"
     CDS             15104..16450
                     /locus_tag="U4A12_001555"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN1773919.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16758..17144)
                     /locus_tag="U4A12_001556"
     CDS             complement(16758..17144)
                     /locus_tag="U4A12_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1773920.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5641 a         2995 c         3422 g         5113 t
ORIGIN      
        1 aatgaaaaaa cattaaggat ggatgaaata tgaataacga aactatcatt ttaggaaaaa
       61 aatatatttg ccaacctatt ggcttgaaac aaaaagtggt tggagaagta atcagtaaat
      121 tagaaaattg tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat
      181 tagaaaaatg cggaaaagta gtggcaaaat atactcacgt ttatgagttg gaaacaacag
      241 aatatctata taactatagc aacaaatatg aacatacttt agtttcataa agatttaaat
      301 aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc actttattta gttggcagtg
      361 attgttcatg atttttgatt tattcaaaag gttcgcctag agaatctgtc tctgtaacga
      421 ggcctggtgg agaaactcga taaaaaccta tactgccaga actgtcctat tttcggatag
      481 acaaactagt agttttaaaa agatcatccg atccaatcat ttcaaaaaat gcaaaactga
      541 tatcaggatc atttagctca ggcttgttat cgtaaagcat ttgtaaaggt tgcatttagt
      601 taggagtaaa cgcgaaacta tcatcattat ttgctgaaga agatgaagaa gctaattcag
      661 agtttgtttg aaataaagga gacgtgctga cagtttcttt accctgtttt ttgcagaggt
      721 agaaactttt atatcggaag tttctaccgg agaatttttc tcatttgtag aaattgttga
      781 atcgatacta ttcgaaattt gcgtatctga aggtatgttc tttgatgcag aagtcccaca
      841 agcagtaagt aacaataaat ttgataaact gataaacaga attcttttca ttatgtacac
      901 actccttata gtatcgttta gcatacacct atgctcagta ttaggcaaaa tcaatgcaaa
      961 actatcaagt cttttatagt atctattagt cttagtaata catatatgga gagggtgata
     1021 tcccagttca aaccagatac attggttacc atctgtatta acgattatgc ctctaaacgc
     1081 tttttttatg aaaaaattta taagtgaaaa cgtaggtatg atattatcta gaggtaattt
     1141 ttttgggtat ttaaatagtt gctaggagga tagcttttgg atttttacag taattttatc
     1201 ttaataatag caatattatt acttctaaat atttggtttt ttgataagag tagaaatgca
     1261 gggataggtt ttagaaccaa acgaagtacg tcttctgaaa aaaagtgggt atattcacag
     1321 actatttttt acggaggggt tatttcaatc agtttacttt cttcaacact ttattctttt
     1381 aatgttatag acgtttccat gtcaaatttt atatctatta ttggaatcct tatttctgca
     1441 attattacac aactacttct tgtgtttgaa gagaagtcaa aaaataatta aagcaattat
     1501 gccttttagt acaggaatta ttacttctta caaatgtata gcgaatgtga agcctgctgt
     1561 tttaattgtt ctagaaaaac ttttgagtga acaattattt tatgttagga tgaattaatt
     1621 tctctagagt ttataattaa aaaacttatt tgaaggaaca tgcttaaacc ataattgtgt
     1681 ttttccttcc atcagcataa aagttcacct caaaaagagt gtacgaacaa aagttctttt
     1741 tgtaaacctt aaaatagatt ttcctgaata cgtattgggg atttctaaat gtttgaatgt
     1801 ttttttgaat gcaaaatcaa tgcaaaaaat tttgtatttc tctacttttc tctccttgac
     1861 caaaataaaa aaacgttgat ttaacaacgt ttttcataca tgtttatctg tctcaaccat
     1921 acatatatgg agacggcggg agtcgaaccc gcgtccaaac acatcgccac ttaaaaatct
     1981 acgttcatag tcaactcatt taaggattca ctttacagct tgccgagtga caggcattct
     2041 gtatcgctag tctgatgttc tctttttcaa cttacagacg gaaagttgaa acgtatccca
     2101 ctaaagttag gacccttact cgagcacatg ggcgatgccg agaggatcta cgctaactgt
     2161 ttttaggcag ctaaagcgta agagttgttt tcgtttttag cagttatatt taactgtaac
     2221 gtttttacgt agacgtaacc tacgaaacgc aattcaagct cgactgtgcc tgtcgaatcc
     2281 gtaacgtccc cgttagctaa caagggttac gtgtattttt catacaaaat tagtatagca
     2341 tatattggga taaattcaat aaaaaagcga atcatatccc attatttata taataactgc
     2401 ccctttctta agaaaaaata agcgattttc ttaagaaaag aggtagcttc aaaaaaagat
     2461 cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc gccttctgta tttttctttg
     2521 tgataaaggt agaaagttct tttaatccgt cataggcgtt gcccatagcg attttgttgt
     2581 cgcaagcttc caacaaggca aaatcattcg gaccatcgcc aaatccaaaa gtaggaactt
     2641 tttccagctg aagttctttt tgcagacgtt tgacacctgc cccttttgag attcctttct
     2701 tcactgtatc gatcgagtaa ggaccattcc gataaaaagt tagctcagga aaatgttcgt
     2761 gatagtattc gtcacctgat tgagataaga tcagcaacat atttactgtg ttttctttaa
     2821 agcctaaagg atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca ttgatcattg
     2881 tttggctatg accggtacac cagattttct gatcgttata gtaagaaagt tcatgaccgt
     2941 tcaactgtac atgatcatac attttttgac attcttctga agaaaatgtt tgagaataga
     3001 tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa gtaatgcccg
     3061 catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg acggggagaa
     3121 cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt tttgatttcg
     3181 catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt gacacaaata
     3241 aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa gacaaacctt
     3301 tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac tataagaaga
     3361 gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt aataattggc
     3421 agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta tcattcgcct
     3481 cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg aaactggttt
     3541 tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc tgggcatacg
     3601 aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat ttttattatg
     3661 acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa tgaatatttt
     3721 aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct taaatgaagg
     3781 ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt tagaaaaccc
     3841 acaacattgg gatatgatta cactagacct gaatttgccc acactggacg gtatgctagt
     3901 aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga cagctagaga
     3961 ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg tcacaaaacc
     4021 tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta gtgagttggc
     4081 agaagcaaga gaagaagcag cagtttcaga tgatcatttt gatatcgaga ctagtcattt
     4141 caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg aaggactgac
     4201 gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag tattctctcg
     4261 ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac ggacagtgga
     4321 cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag tcatccaaac
     4381 tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg ttatttgtat
     4441 cagcaactct tagctttttg cggaatggtc gcgttgatct tattgatcgt tggagtttct
     4501 tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact gtttggatat
     4561 gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca gtttagtcaa
     4621 gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga acaaaatgtg
     4681 aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac agctttactg
     4741 aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca agaaaaattc
     4801 acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact tgtgccattt
     4861 aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg aaatatcgat
     4921 aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc cagtatcgtt
     4981 gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa tcggttaagg
     5041 aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt acatgacaaa
     5101 gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt gaaagaatct
     5161 caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga tgcttctcac
     5221 gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga atacaatgca
     5281 atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac agagcgtctg
     5341 atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca aatcagtatg
     5401 gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca gttagaagct
     5461 aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat tgatgtttac
     5521 gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc gatacagttt
     5581 actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac gatcatcacg
     5641 attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg ggatcgttat
     5701 tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt aggtttacca
     5761 atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag cgaactagga
     5821 aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga gtaaaaaaca
     5881 agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta taaaatgatt
     5941 gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag tagcatcatg
     6001 agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca agtggagtag
     6061 ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa tattgtttcg
     6121 cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg tcaactcagc
     6181 tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg ccaaacgatc
     6241 ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat tcagataagg
     6301 aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa caagaacctc
     6361 ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa taacggtcaa
     6421 gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta ctttgcaaat
     6481 gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca ctgagcccaa
     6541 taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt tggaaatcga
     6601 tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa gtcaagatat
     6661 cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt tgaagaagcg
     6721 cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa caaagaaaga
     6781 gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa agaccagtca
     6841 ggcgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag aaaaagataa
     6901 aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat cggctagcta
     6961 attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa agccgatctt
     7021 ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct atcaataaaa
     7081 aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa taaattaatc
     7141 gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt atagaaaatg
     7201 aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca gaaaatattt
     7261 tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga tgcaagcttt
     7321 tgaacactgt ttattcgaaa agaggagcag cgacaataat tttgtcgctg ctccttttaa
     7381 atttattttg tcattcggaa gaacttgtgg tcgttccgtg aagttcttgg tacgtttgcg
     7441 ttttataaag atcaacagtt gattgtccgt tgtgttcact aaagacacta gttgacttgt
     7501 caccaagaga tttttcagct tcttttaatt tctctaagcc attcttgtag tcataatcac
     7561 tggtatcaat tgggtctaat ccactattcg tatagaaccg aagaagatca ccattattga
     7621 tttggtcaga aacagctaac tgtttatcaa ctttttcttt cagtgaatca atttcattct
     7681 gtacttcctc agtaggttca gtaatagctt caccagtttc gttactatag atcgtaccac
     7741 cgtagtaagt atattttggt gtaacaaaat caccatcacg gaaagaaaca agctgttcat
     7801 gatctttcga gaatagatct tgtcctaact gaatataatt ttttgtatct acacctagta
     7861 agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta agtgtgattg attccgccct
     7921 gtgtttgtcc agggatatgg atcatataag ggacacgctg catttgtgca ttgtcgtagt
     7981 ttgtccaagt agaacttgtt tttccaacta attcagctag atttttattt cttgaattcg
     8041 agacaccgta atggtcaccg taaaggacga tgactgaatt atcatacaag ccgctttctt
     8101 tcaaataatt gaagaattct tgtacggcgg tatctaaata attggctgtc gcaaagtaac
     8161 cgttgatcgt ttcatcattt gtattggcta acgggaatcc tgcttcgtca ttggtgaatt
     8221 ttgaataagg ataatggtta gatacagcaa tgaattttga ataaaatggt tgttgcagac
     8281 gttccaagta ttgtacggat tgttggaaaa atggtttatc atgcaacccg tattggaacg
     8341 aattgtcatc attaacatca taataagaag aatcaaagaa ataatcatat cctaaattct
     8401 tgtatgtctc gtttcgattc cagaatgtac cagcgttccc atggaatacc gcactagtgt
     8461 aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa agtatttttt ccgcctaatt
     8521 gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt ttccatcagc gtttccgcat
     8581 cgcttgtttt tcctgctttt acttgatgga aaaagttgtc aaaactgaac gtgctgttgc
     8641 tgtggtaaag actattgatg aaaggtgtaa cttcatgctg gagaccattc tcatctgtca
     8701 atttataatc gatcaggaat tgctggaagc tttctaaatg gatataaatc acattttttc
     8761 ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata atgttctttt acataatttt
     8821 ctacttcttt catatcattg ggacttgctt ctgcccgcac ttggtttgtc tggtaagttt
     8881 gaacaccgtc ataaaccatg aaagcattca agccaagata tttgaccaaa taatcacgag
     8941 agaatgttcg agtcagcaat tctggacgat cagcttccgc cataaaaaga tttcctgaga
     9001 aaatcataac tgctaatgta gagatcgcaa aagccattct tgctcggatc gggcgagggt
     9061 ccaacttgat tttttttgca atcagcagaa cagtcaagat aataaaatcg atccaataga
     9121 tcacatcata tgggcgaaac agtcgaatgg cactttctcc cagaccactc gctactttcc
     9181 cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa ttctcgataa taagtgacat
     9241 tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat catcgtgata taagcaactc
     9301 tagggcgtct gatataaaga gcaattccaa gtaataacaa tgtagttgca atagggttga
     9361 taaacaagat aaagtattga atgccacttt cgattccgag atggaaatca actaaatagg
     9421 caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga aaaaaatccg agcctcttat
     9481 tcaaaaaggc aggtgttttt atacatttca agataatctt cctttctgca cttcttaatc
     9541 acagaagtgt catgaaacca tgttacacag ctcacttatt ctcatttatt ttactagtat
     9601 gacaatatag tgtcaattga aaccagaagt ttatgctgtt tcattaaggt tttctttaag
     9661 cttcatgaaa aaatgagcag taagacaagt cctatgtttt ttgttatact tgggaagaga
     9721 aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa aagagcaagc aatcgattga
     9781 aaaaaggata tccactgatc caaaaagaag accttgctca tccccaaaaa gaaaccacgc
     9841 aatgggtgga attagttgat tcgcaaaacc aatttatcgc aaaaggatat ctaggaaagc
     9901 agaataaggg aatcggttgg gtattgagcc agcagaacat cccatttgat caatcttttt
     9961 ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt ctttttttca gatgattcta
    10021 ctaccgcttt tcgtatattc aatggagaag gagacggaat cggcggtctg attgttgatt
    10081 attatgcagg ttatgctgtt ttttcttggt ataacgaaac gttatatgca catcgtgaat
    10141 tattgattca agcttttcgt gaaatctacc cggaaatcgt aggaggctat gagaaaattc
    10201 gatttgaatc agtaaatctg cctgagagcc agttcattta cggaaaagag gcaccggaac
    10261 ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta tttaaatgaa ggattaatga
    10321 ctgggatctt tttagaccaa aaagaagtac ggggatcatt agcagaaggt ttcgcttctg
    10381 ggaaaacagt tttgaacatg tttagttata ccggcgcctt ttctgttgct gccgcaatgg
    10441 gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg cagtttaaaa aaaacacagg
    10501 aacagtttga agtgaatggt ttggatgtcg atcagcaaaa aatcattgtt atggacgtat
    10561 tcgagtattt caaatacgcc aaaagaaaac aatttttata tgacatgatt gtattggatc
    10621 caccaagttt tgccagaaat aagaaaaaag tctttcgcgt agcaaaagat tatggggaac
    10681 tggtcaagga tagtctggat attttagcag aagacgggat acttattgcg tctacaaatg
    10741 ctgcgaatgt ctcgatagaa caattccaaa caatgattga agaggaattc catgccaaaa
    10801 atgtttcttt tgaacaactt gctttatatc gtctgccaaa agatttccag acgacggata
    10861 cctttttaga aggcaattat ctgaaagtgt ttgtttatca aattaaaaat aaccattaga
    10921 aggagcgatg cagaatggtt tgggtaaagg gattgaatat aggaagcggt agaccaaaga
    10981 tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt acgtgaagca gttcttgcac
    11041 gtgaagcagg ggccgattta gtcgaatggc gagtcgatca ttatcgtgaa gttttttctt
    11101 ttgatgaagt agcagaaaca ttggttctta tccatgagat gcttgatgga ctgccgttat
    11161 tatttacgtt tcgtacctta gctgaaggcg gagaacaaga catatcgatc cgtcagtatc
    11221 gcgaccttta tgaacatgtg atacacacag gattagtaga tatggtcgat atcgagttgt
    11281 tcaaagtaga aagtctagga aaaagactga tagaggaaat caagtcttta aacattcctt
    11341 taattatcag tagtcatgat tttaaagaaa caccagcaga ccctgtcttg ctttaccgat
    11401 tgaatgttat ggaacatttt ggagccgata tcggaaaact ggcagtaact cccaataatg
    11461 aacgagatgt tttgcggttg atggagttga ctagacgggc aaatgctttt gtctccatgc
    11521 ctattattac gatgtccatg ggaaatcttg gtaaaatttc tcgtctggct ggcggaacga
    11581 cgggttccgt tatgacattt ggttccttaa gcagcgattc tacttctgct ccaggacaat
    11641 taccggtcaa agcattgaag caaattattc aattattaaa tgaaccgttg tagaacaagt
    11701 ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa cgaatgcaat ccgtatggtc
    11761 gaacaaaaaa agattcccta tacagagcac gaatatgagt gggacgaaag ccacctaagt
    11821 gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc gtatatttaa aacactggtt
    11881 gctgtgggca atgtgacggg gcctcttgtt gctgtgatac cgggagaagc cgaattgaat
    11941 ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag aaatgctcca tttaaaagat
    12001 ttggaagcaa cgaccggata catacgtggt ggctgctcgc ctataggtat gaaaaaacta
    12061 tttcctactt atttagatca aattgcagaa agctatgaac aaatcattgt ctcggctggc
    12121 agaagagggc tacagatgga gttagcacct caagatatct gtgcattaac gagcggacaa
    12181 tttgcagata taaaacaata aatcatacgt ataaaagaaa gaaggaggcc gggatgttgt
    12241 ttgtccagtc tccttctttc ttttttatac attatgctct ttgcccggca ttcattcctg
    12301 ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc gccagtataa ccattgacat
    12361 ccagtaattc tcccgcaaaa tacagaccat caatgatttt actctccaat gttttaggat
    12421 aaatttcctt tagagaaacc cctccgccag ttacaaatga tttctcaata ggaaaagttt
    12481 tgttgattga tagcttaaac tctttgcata gttgaacaaa atcttgaatt tgtttttgag
    12541 aggcttgttg tcctgtcaga tgatccattt caagccgctc aagaaagaaa actaacaatc
    12601 gctcaggcaa tataccatgc caagcattga ccaagttctt tttggttgct ttactttttt
    12661 ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc agagaaagtg
    12721 tcactggttc tcccgttttc tctaattctt tattgacaaa acttgaacaa cgcagtgcag
    12781 ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca gtcaatacac
    12841 gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct tgaagtgttt
    12901 tctcctgaat atacggttca tccgatatca agggggactc tgtcgcataa agaggcgtca
    12961 ccgtgtgacc cactcgcttc acgatcttgt acccatctcc agtagaacca gtagatggat
    13021 aagtccgtcc accagttgtt aagataatgg caggagcatg gaactcttcg aattccgtgc
    13081 gtataccgta aattttatgt tctttgtgga tcaatttctc tactcgagta gagaatagaa
    13141 gcgtcacatc tagttctttc agacgattga gcaatgcttc gataatcgtt tttgatttat
    13201 ttgttacagg aaacatccgt ccatgatcct cttcttttaa atgtacgcct tgtgattcaa
    13261 agaagttcat gatatcgaaa ttattccatt gcgcaaacgt actatataaa aactttccat
    13321 ttccaggaat atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca ttgcaccgac
    13381 caccacctgt catcagtagt ttttttcctg cttttttgtt tttttcgatc aagagtacgc
    13441 gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc gcttgttcca gcgccgacta
    13501 cgatcacatc atacagtttt tccattccct cacctcaagg aaagtgtagc atatctgaag
    13561 aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa atgagctaaa atgagggaag
    13621 ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa tatgttcaag caattcaaga
    13681 aaagctacat caaaaggatc aagggcaaga agaattttta caagcaatcg atgaattcat
    13741 gcccacggtt atgtcttttt tggacactca tcccgaatat atcgaaaaga atatcttaga
    13801 acttttaacg gaaccagaaa gagttattca atttcgagtg ccttggcaag atgatgaagg
    13861 aaactggcga gtaaatagag ggtaccgtat ccaatacaac tcagctatcg gcccttataa
    13921 aggtggattg cgcttccatc ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga
    13981 acagattttc aagaatagtt taaccggtct gccaatcggt ggaggaaaag gcggaagtga
    14041 cttcgatcct aaaggaaaat ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct
    14101 ggaattgtca aaacatatcg gcccatcgat tgatgttcct gcgggagata tcggtgtagg
    14161 agctagagaa atcggctatt tgttcggcga atataaacga ttgaaaaaat atgatactgg
    14221 tgtgttgaca ggtaaacctt tggatttttg gggaagtaag atacgtacgg aagctactgg
    14281 ttatggatta gtgtattatg tcaaacatct attgaacgaa gaaaaagatt catttaatca
    14341 aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca
    14401 agagctaggc gggaaagttg ttacatgttc agattctagc gggtacatct atgatccaga
    14461 aggaatagat gtgaaactat tgaaagaagt caaagaaatc aatagacaac gtttgacgga
    14521 atacgcaaac caacgtcctt ctgctattta tcatgctgga gaatctgttt ggacattgaa
    14581 agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat gaaatagacg aaagcttggc
    14641 cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac
    14701 agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc
    14761 taatgctgga ggagtagcag tatctgcttt agaaatgagt cagaacgtcc agcgattgac
    14821 ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc atggaaacga tttaccatac
    14881 gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt
    14941 tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa
    15001 actttcttgg aaatattgtc agcattttgt ttatttgctt gaaaaataat aaaaaaaaac
    15061 gtaaagtatt acctgtagag taaacctata aggaggagaa caaatggcac acattcattt
    15121 tgactattca aaagtagcac cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca
    15181 agtaacagca gcccacaacg aattacggga aggtactggc gcaggaagcg acttccgcgg
    15241 ctggatcgat ttaccaacga actatgacaa agaagaattt gcacgtatca aagaagcagc
    15301 taaaaaaatc caatctgatt ctgaagtatt agttgttatc ggtatcggtg gttcttattt
    15361 aggcgctcgt gcagcgatcg acttcttgaa tcacacattc ttcaatttat tagatagcaa
    15421 cacaagaaaa gcaccacaaa tcttctttgc cggaaattca atcagctcaa catatattgc
    15481 tgacttgatc gaagtaatcg gtgatcgcga cttttcagta aacgttattt caaaatctgg
    15541 aacaactact gaaccagcga ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata
    15601 tggacaagaa gaagcaaaca aacgtatcta cgctacaaca gataaagcaa aaggtgcagt
    15661 taaagtagaa gcagatgcag aaggttggga aactttcgtg attcctgatg acatcggcgg
    15721 acgtttctct gtattaactg ctgttggttt gttaccaatc gctgttagtg gtgcagatat
    15781 cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca tactcaagcg acaaactaga
    15841 agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt
    15901 aactgagttg ttgatcaact atgaaccagg aatgcaatac ttctcagaat ggtggaaaca
    15961 attgtatggt gaatctgaag gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt
    16021 ctcaactgat ttgcactctt taggacaatt catccaagaa ggacgtcgca atatttttga
    16081 aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca attcctgaac aagcagaaga
    16141 tttagacggt ctaggctact tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt
    16201 tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct aacttgctag tcaaaattcc
    16261 agaaatggat gcttatacat tgggttatac aatgtacttc tttgaaattg ctgtaggtat
    16321 ttcaggttac ttgaatggaa tcaacccatt cgaccaacca ggtgtagaag cttacaagaa
    16381 aaacatgttc gcacttctag gcaaaccagg ctttgaagat cttgctaaag aattgaacga
    16441 acgcctgtaa gataagggtt tttcgctaga tttaaataat cagtgataga aacatgttcg
    16501 ctttaaaatg aaaactggta cccatttagg tactcaaaca tactgcatcg tttattcaga
    16561 ataaggggct gtgacaagac gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt
    16621 gccttgctct ttagtttgtt ttaggacttt aagaacagtg aggctgactt cgaaggttaa
    16681 ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt
    16741 tgcgatatat tcttagctta agataggcct aatttttctt ttacagatga gggtacctta
    16801 tcgctggtaa ttactttttt ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc
    16861 gttccttcgg ggtaagcaga accattggca gagtcaccag aagtaatgac atcgaaatct
    16921 aactgtcttt tttcaccgtt ttcagcataa gcatctacgt tgtatttaaa acctcttcca
    16981 attacttccc cattatctgc tttgatatca gtttctgcag gcattggagc tttgactacc
    17041 gcataataat ctttacctac ataagtagac tgatagtagt cccaaccttt ccatcctata
    17101 aaagcaatca atatgataac gatcaaccct aaaatttttt tcatgaaaga tctccttttt
    17161 tatttttatt a
//
LOCUS       DASJOX010000049        17140 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJOX010000049 DASJOX010000000
VERSION     DASJOX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.050"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..1475
                     /locus_tag="U4A12_001557"
     CDS             138..1475
                     /locus_tag="U4A12_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1773921.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="U4A12_001558"
     CDS             1523..3652
                     /locus_tag="U4A12_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN1773922.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="U4A12_001559"
     CDS             3627..4316
                     /locus_tag="U4A12_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773923.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="U4A12_001560"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="U4A12_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="U4A12_001561"
     CDS             complement(5546..5740)
                     /locus_tag="U4A12_001561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773924.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="U4A12_001562"
     CDS             complement(5790..6230)
                     /locus_tag="U4A12_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN1773925.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="U4A12_001563"
     CDS             complement(6234..7079)
                     /locus_tag="U4A12_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN1773926.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="U4A12_001564"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="U4A12_001564"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN1773927.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="U4A12_001565"
     CDS             complement(8703..9650)
                     /locus_tag="U4A12_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1773928.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="U4A12_001566"
     CDS             complement(9873..10223)
                     /locus_tag="U4A12_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1773929.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="U4A12_001567"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="U4A12_001567"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN1773930.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="U4A12_001568"
     CDS             11646..11966
                     /locus_tag="U4A12_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1773931.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="U4A12_001569"
     CDS             11988..12452
                     /locus_tag="U4A12_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1773932.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="U4A12_001570"
     CDS             12657..13262
                     /locus_tag="U4A12_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN1773933.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="U4A12_001571"
     CDS             complement(13301..14590)
                     /locus_tag="U4A12_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN1773934.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="U4A12_001572"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="U4A12_001572"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN1773935.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="U4A12_001573"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="U4A12_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17140
                     /locus_tag="U4A12_001574"
     CDS             16321..>17140
                     /locus_tag="U4A12_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEN1773936.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT         5455 a         3105 c         3466 g         5114 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
//
LOCUS       DASJOX010000050        16643 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJOX010000050 DASJOX010000000
VERSION     DASJOX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.051"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..258)
                     /locus_tag="U4A12_001575"
     CDS             complement(<1..258)
                     /locus_tag="U4A12_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773937.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="U4A12_001576"
     CDS             complement(274..924)
                     /locus_tag="U4A12_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773938.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="U4A12_001577"
     CDS             complement(935..2107)
                     /locus_tag="U4A12_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1773939.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="U4A12_001578"
     CDS             complement(2126..2998)
                     /locus_tag="U4A12_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1773940.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="U4A12_001579"
     CDS             complement(3020..3940)
                     /locus_tag="U4A12_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773941.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="U4A12_001580"
     CDS             complement(3940..4209)
                     /locus_tag="U4A12_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773942.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="U4A12_001581"
     CDS             complement(4206..4586)
                     /locus_tag="U4A12_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEN1773943.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="U4A12_001582"
     CDS             complement(5053..6516)
                     /locus_tag="U4A12_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1773944.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="U4A12_001583"
     CDS             complement(6580..9597)
                     /locus_tag="U4A12_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1773945.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="U4A12_001584"
     CDS             complement(9748..10587)
                     /locus_tag="U4A12_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1773946.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="U4A12_001585"
     CDS             10732..10977
                     /locus_tag="U4A12_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773947.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="U4A12_001586"
     CDS             11040..11462
                     /locus_tag="U4A12_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773948.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="U4A12_001587"
     CDS             complement(11556..11873)
                     /locus_tag="U4A12_001587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773949.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="U4A12_001588"
     CDS             complement(11900..12106)
                     /locus_tag="U4A12_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773950.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="U4A12_001589"
     CDS             complement(12087..12269)
                     /locus_tag="U4A12_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773951.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="U4A12_001590"
     CDS             complement(12283..12633)
                     /locus_tag="U4A12_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773952.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="U4A12_001591"
     CDS             complement(12659..12961)
                     /locus_tag="U4A12_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773953.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="U4A12_001592"
     CDS             complement(12964..13197)
                     /locus_tag="U4A12_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773954.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="U4A12_001593"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="U4A12_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="U4A12_001594"
     CDS             complement(14628..15743)
                     /locus_tag="U4A12_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1773955.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="U4A12_001595"
     CDS             complement(15749..16147)
                     /locus_tag="U4A12_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1773956.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4433 a         3458 c         2588 g         6164 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact ctt
//
LOCUS       DASJOX010000051        16492 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJOX010000051 DASJOX010000000
VERSION     DASJOX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.052"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1452
                     /locus_tag="U4A12_001596"
     CDS             1..1452
                     /locus_tag="U4A12_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1773957.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="U4A12_001597"
     CDS             1801..2433
                     /locus_tag="U4A12_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN1773958.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="U4A12_001598"
     CDS             3021..4460
                     /locus_tag="U4A12_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1773959.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="U4A12_001599"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="U4A12_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="U4A12_001600"
     CDS             5222..5437
                     /locus_tag="U4A12_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773960.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="U4A12_001601"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="U4A12_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="U4A12_001602"
     CDS             complement(6097..7008)
                     /locus_tag="U4A12_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1773961.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="U4A12_001603"
     CDS             complement(7027..8031)
                     /locus_tag="U4A12_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1773962.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="U4A12_001604"
     CDS             complement(8028..10151)
                     /locus_tag="U4A12_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1773963.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="U4A12_001605"
     CDS             complement(10156..12603)
                     /locus_tag="U4A12_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1773964.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="U4A12_001606"
     CDS             complement(12590..12979)
                     /locus_tag="U4A12_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1773965.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="U4A12_001607"
     CDS             complement(13039..13542)
                     /locus_tag="U4A12_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1773966.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="U4A12_001608"
     CDS             complement(13555..13779)
                     /locus_tag="U4A12_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773967.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="U4A12_001609"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="U4A12_001609"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1773968.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DASJOX010000052        16299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJOX010000052 DASJOX010000000
VERSION     DASJOX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.053"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            775..1161
                     /locus_tag="U4A12_001610"
     CDS             775..1161
                     /locus_tag="U4A12_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1773969.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1255..1446
                     /locus_tag="U4A12_001611"
     CDS             1255..1446
                     /locus_tag="U4A12_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773970.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1960..2292
                     /locus_tag="U4A12_001612"
     CDS             1960..2292
                     /locus_tag="U4A12_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1773971.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2202..2396
                     /locus_tag="U4A12_001613"
     CDS             2202..2396
                     /locus_tag="U4A12_001613"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1773972.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2588..2743
                     /locus_tag="U4A12_001614"
     CDS             2588..2743
                     /locus_tag="U4A12_001614"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1773973.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3019..4359
                     /locus_tag="U4A12_001615"
                     /pseudo
     CDS             3019..4359
                     /locus_tag="U4A12_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4600..6216)
                     /locus_tag="U4A12_001616"
     CDS             complement(4600..6216)
                     /locus_tag="U4A12_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1773974.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6334..6552
                     /locus_tag="U4A12_001617"
     CDS             6334..6552
                     /locus_tag="U4A12_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773975.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6747..7208
                     /locus_tag="U4A12_001618"
     CDS             6747..7208
                     /locus_tag="U4A12_001618"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1773976.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7401..7640)
                     /locus_tag="U4A12_001619"
     CDS             complement(7401..7640)
                     /locus_tag="U4A12_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1773977.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7664..9187)
                     /locus_tag="U4A12_001620"
     CDS             complement(7664..9187)
                     /locus_tag="U4A12_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1773978.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9205..9327)
                     /locus_tag="U4A12_001621"
     CDS             complement(9205..9327)
                     /locus_tag="U4A12_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1773979.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9357..10340)
                     /locus_tag="U4A12_001622"
     CDS             complement(9357..10340)
                     /locus_tag="U4A12_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1773980.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10364..10513)
                     /gene="rpmG"
                     /locus_tag="U4A12_001623"
     CDS             complement(10364..10513)
                     /gene="rpmG"
                     /locus_tag="U4A12_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1773981.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10534..10911)
                     /locus_tag="U4A12_001624"
     CDS             complement(10534..10911)
                     /locus_tag="U4A12_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773982.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10943..11122)
                     /gene="rpmF"
                     /locus_tag="U4A12_001625"
     CDS             complement(10943..11122)
                     /gene="rpmF"
                     /locus_tag="U4A12_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1773983.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11137..11406)
                     /gene="rpsN"
                     /locus_tag="U4A12_001626"
     CDS             complement(11137..11406)
                     /gene="rpsN"
                     /locus_tag="U4A12_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1773984.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11929..13695)
                     /locus_tag="U4A12_001627"
     CDS             complement(11929..13695)
                     /locus_tag="U4A12_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773985.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13655..15408)
                     /locus_tag="U4A12_001628"
                     /pseudo
     CDS             complement(13655..15408)
                     /locus_tag="U4A12_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15518..16087)
                     /locus_tag="U4A12_001629"
     CDS             complement(15518..16087)
                     /locus_tag="U4A12_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN1773986.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16105..>16299)
                     /locus_tag="U4A12_001630"
     CDS             complement(16105..>16299)
                     /locus_tag="U4A12_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1773987.1"
                     /translation="ERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKD
                     IKLRVEDSTLIEQIFNDITK"
BASE COUNT         5284 a         3102 c         2453 g         5460 t
ORIGIN      
        1 gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa acatgaagag actttaataa
       61 gacttcttta tctgaaaaca cttttgtttc tctctttcct ttctacatca ttaaatagta
      121 ttttatacgt tatctaaaat actattcctc tgtcaaaata agctaactta cttcattata
      181 aaatatccac ttatcgcttt catgtatatt ccaccgtaaa aagtacattt tatcaccgag
      241 aactcatcat ttttcatcat gatgaatact atttacatat atctagttca aagacaataa
      301 ttattatgat ccgttattat ctaagcacta tacagataat aggaattttt cgtttctaaa
      361 tattccaatt tgggaatcca ataaaaattt tcgttcaaat acgtgtctat tcgaactctt
      421 ttctagttgt taattatgaa gggggaagaa ataaaaaata caatctatac acgaataaga
      481 atattatcca aaaggaggtt ttagaaaaga tttattatac gtaaaaagct caagacattt
      541 tcttagccat aaggacatgt ttgatattta agaagatata tgaattttct tttctaagta
      601 taaagataac tatccattat caactaaaaa aacatttttg aaaataaaca tcagcagtcc
      661 tatcaatgca tgtgagctta tagaagttat tcgcagcgca gcataattgc attagcttct
      721 tttcgtgttt tttgcctctt ttccctttaa atcttaatgg aggaggtgtg caatatgtca
      781 gataacgacg aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt cattttgaac
      841 accgctaata cttattataa aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa
      901 ctcccaccat atttgtttac taaagatact ataaaattta aacaaccgat taatgtttta
      961 aatgtgactc tccttgttga agatataaaa ttagcaaaaa tccttccttt gttgaaagaa
     1021 cctgaacaaa tttttcttat cgaaaaattt atctttgata aaactgataa agaaattgga
     1081 aaggttctgg ggattacaag acagggtgcg acaaatttaa aacatcgact ttacaaaaaa
     1141 attcaaaaaa tgctggttta atatctgcat aataactaat tttgtatgct tctttgctcg
     1201 ttctcctcct tcccaaagaa atagattata agtccatggg aagaaggtag atttatgaat
     1261 tctttaaaag aagatttcat tttagcaaag gctggaaatg aagaagctgt tgaggcaatt
     1321 ctcaaaagat ttagctcttt gattcataag caatcctgga gaactggaaa atatgatcaa
     1381 gattgttatc aagaatgtat gcttgcaata tatctagcta tctcgaaatt cgaaattaaa
     1441 gaataactca actaagggag tctgtgacat aagtaaaaag atagtttcaa atttcgaaca
     1501 ataaatttga aactatcttc tttataatta taaagtatcg attttacagc gttctataat
     1561 gattcataga atctaaaacg aatgatttta attatcaacg ttttttaatt cgtttttagc
     1621 ttaaaaataa cttttgtcac agtctcactc ctaaaattac tttatttacg tatgaagtgg
     1681 gcaggaggtc gaatccagaa ctgggctatg gtacttcaac gaggacttct cctctagaat
     1741 tgaaaagcat gcgatttacc ttccgtaacg acggtagatt tattcaacta tgtgtccaat
     1801 caaaatttgt ctgatttcaa aaatagtcaa gagaattttg gttgttaaaa actcttatct
     1861 atcaacttcc acagtttagt tgacaaaccc tgatactaag ttgggctatg atacactttt
     1921 cttgactatc agttacgtta ccggaaggaa tgaactctat tgagaaaaac atatgatcgt
     1981 aaatttaaac taaagatttc acaggatatc cttgagaaaa aagtaactac caagaaaatt
     2041 gctgaagaat acaatatctc tcgtccaaca atttcccgat gggtttcaga atatcgtcga
     2101 tacgggaaaa atgcttttgc tggacaagga aaaagattac ctgataaagc agatttctac
     2161 attcttgaac aggaaaataa acgactaaaa gaggaagacg agtgttctac gaacacaaag
     2221 atcgatacgg atctccaggg atcactcaaa aactttacga tgaaggaatc gaaatcaata
     2281 aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg cacaagagga tttcatcgac
     2341 gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat ggtaaaaaaa tgttgaatcg
     2401 ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg gatattactt acattccatg
     2461 taatgacggt cgcctctatt tgtccaccta tattgactta gcaactcgta ttctacgtta
     2521 ctatatggta gattcacaca tgaaaaaaga gattgtcgtc cgtccattac aaatttatta
     2581 aggagaattg cctacggtaa ttcattccga tagaggtagc cagcatcggt cttttattta
     2641 tcaagagctg ttgaacgacc accacatcac acattcagtg agccaaactg ggactcctgt
     2701 tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa
     2761 caaatataaa agtaaaatag agatgaaggt gttaatccaa gattatttag aagactacta
     2821 cccaaacaaa agaatccata caaaatttat gatgactcct tgacaatttg aagcgaagaa
     2881 aataagggat ctataatatc tagtgttggt gacgccttct gttgccaata ctttttcttt
     2941 tttctaataa acgcaaacag acaccttaaa atcctttaga ataaagtcga cgaaaaccat
     3001 tcaaaggagt gtttcaagat gtcctattca gagtttacca aagagttact agatattcta
     3061 gacctaaatc ttatctttca tgaagatgcc tttcgcaaag agcgaatcaa tgacgaaaca
     3121 tgttttgttt ttgatggcac attgacctat caaccggagg aatgctttca tcgccactat
     3181 caaaatgaac aaacgatcat taaatagggc tggaaaaaag tatccatctt attgaatgat
     3241 gtcagtaact acaagaccat tctgcggatt aacaaacagc gcttcaaatg taaacactgt
     3301 ggaaagactt ttttagcaga agattcagtt agtgatcgtc gctgctccat tgctcgcaga
     3361 gtcaaacaag ccattcttga gctattaagc gaacctcttt ctatgtcttt gatcgcacgt
     3421 atgaagcaca tttctccgac cactgtcatt cgaatacttc gcagccttcg gcctaaaacc
     3481 atttctttga gccagctgtt accagaagtg gtgtgtttcg atgaattcaa atcagtgaaa
     3541 aatgtctcgg gagccatgag ctttgtcatg atggacggca agacccatca actgattgat
     3601 attgtggaaa atcggcaact caatccttta cgcgactatt tcttgcgcta ccctataaaa
     3661 gtccgtgaac aagtgcgtct agtggtttca gatttctata caccctatcg cacattggtg
     3721 aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct ttcatattag ccaacacatt
     3781 ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt
     3841 cggtcgaagc acttaaagaa atactggaaa ctacttcaaa agaatgcgtg ggaattgaaa
     3901 ggtcagcatc gctactggcg tccgtcattt cgagatcatc tgacagaagc agagatagtc
     3961 gatcggctat tatcttatga tgattcctta aagcgaggct acgaagtcta ccaggatttt
     4021 ctttccgtta tcagaagaca agatgttcct gaattcgtcg cgctcctaaa agaagactac
     4081 aaggagttgc cagagcacta ccaaccagtg ttcacgacct tcaagaaata ccaaacagaa
     4141 atcaaacgag cgttgcgcgt tccctattcc aacggcccta tagaatgttt gaataaccat
     4201 atcaaagtac taaaacgaat tgcctatggc tatagaaact ttcaaaacta ccgagaacga
     4261 atctttttat atcgaggaaa atattttaag aaaactaaga atactaccca actgactaaa
     4321 gccagaacaa caacacggct agacaagata gctgtctagc cgtgtgagta tctcgattat
     4381 tctattgaat ttcttcacca acactcattg acgaagagcc aaaataagta atctaaccga
     4441 ctgttaacta aaccattgca ggtcccgtta aagatacttg tgcctgttgt caccgcttct
     4501 ttttcttgtc cacaagcgct taacacactt aataaagccc ctgctattat taataataat
     4561 atctttttct tattaaaaac ctcctcatct atttccagat tagtaaatgt agtaatctgt
     4621 gctttctttt ttctcaatac taataactaa cttatggggt aaacaaatac tcgtttgacc
     4681 gggttttgag atccatccag ttctaaccgc gatttgatca ggactattat cttctttaac
     4741 tcgaattctt ccatctttaa tctcaacaat attatattgt cccttcgcag gatgataatt
     4801 cacgagttta tgattgatct tatccaaatc gaagcgatcc atttccttgc catcaattcg
     4861 aacgatggca tatttgatag catcttcagt tgtttcattc tccttttcta tagagaatac
     4921 aaataaggga ataaaagagg taataatcaa aatgccaata attatgacat cgcctatctt
     4981 gacaccagct tttcttaaaa atttcattaa cgatatctgg ctaaacatat tagacttttt
     5041 ccatggaaat ggtgaaattt ttcggaaatt atctgcatta agagaatcag ttaaacaacc
     5101 ctcaagttgt tccttaatac attgttgatc catcttcata ccaataaaaa ctagtttatt
     5161 aatgcgttca ccaaacaacg gatgccaggc ttccttcaat tcgggatttt tttgtataat
     5221 catttgacac tcaattttag gtaaactagc tacccaataa tttaatgatt caatactttt
     5281 atagctaccc gatgtttcaa agatcgtcga aaaatcacta tgttgggcaa accaaagaaa
     5341 cccttttgct cttgtaatat tgccaggaaa atttttatcc gtaagatggg caaatttttg
     5401 tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag ataccatatt cttctgtttc
     5461 aggaatatga ttttgatagc caagctctaa ctctttgaac cagcccactg aataggaagt
     5521 ctcatctata ttaaaaagat ttgtatttag aattttcgat aagtctactt cacctttaat
     5581 tgtcctaatt attttggcat tggactgtaa cttagcaatt agaccgttaa ttttgtaaat
     5641 cgtcttggtt gacactaagt cactattatt taaaagtaat atgttacaac actcgaattg
     5701 atcaattaac aagccacgaa tatcctgttc ttcttcaact acattacctt cagcttttct
     5761 ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa cgttttacat ccactactat
     5821 aataatggca ccaatctcta atcgttgggt caaatcaatg tctaaagggt ttttagctaa
     5881 tataattgct tgaataattg gaattggttt gctgattcct gaagcttcaa taacaatttg
     5941 attcaagtca gaaaattgca ctaattgatc tagttcaaca atcaaatctt cgcgtaatgt
     6001 acaacaaatg catccattcg gcatcgaaac aagtttttct ccagttcttt taaaaccttt
     6061 cttgtcgact aataccccat caacatttac ttcccctatg tcatttacaa tgacagcaat
     6121 ttcctttccg tgatcaccgt acagaatatg atttagaagt atcgtttttc ctgaaccaag
     6181 atagccagtt aagattgtaa taggtatttt tttcataggg aacctctttc tataccttga
     6241 atttacactt taagtgcaac acataaaaat gagtcataat cgatatccaa ctagtataag
     6301 tttgtcgaga acactcctag aaggaaaata attatgagcc agtatcgtca tcttactata
     6361 ctcgaacgag aaagaatctt tctgcttcac gaagaaggat tttctattag tataattggc
     6421 aacgacattc agcatagccc gtttactatt ttacatgaac tgagaagaaa caagaaaaaa
     6481 actattcacc ttccaaagcg gaaaaaaatt atgcttatcg aagaaaaaaa tgcggacgtt
     6541 atcacattct aaatgatccg tgagtttggt caattgttcg tcatttattt gtcgattttc
     6601 agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga acatatggac ttttcaatta
     6661 gttttatcac gatctaacgt gcaatttgca atgatctttt cgatacagaa cccctctcac
     6721 acggcaataa aggcctgatc cgttcattgc accatcgagg aaaaacaaga catacaaaaa
     6781 atcatgtcga gtctcgtgga aagatttgcc ttactcattc gatccatggc cgtccaaaat
     6841 ccgcagatga tagaagtgat tttggtcatt ggaaagccga tattgtagct ggcaaaactg
     6901 gtggtacttg tttagtgact ttgacagaca gaaaatcatg cttcctttta gcaggaaaga
     6961 aggtccccag aaagaaagcc gcttatgtga gcgagaaaat gattgaactt ttaagcagta
     7021 ttccaaatga ggcggtcaaa acgatcactc cagatagagg aaaagaattc tccaatcatt
     7081 tcgcagttac agaagcattg aataatatgc ccttttattt tccagatcct catgcccttg
     7141 gcaacaatga acaaattaaa atacgaatag actcattcga gaatacttgc caaaatcgaa
     7201 agagatagct acggtagata actcaacgat aaatcagatg attgaaaaac tgaataacag
     7261 acctagaaag tgtttagatt ggagaacacc ttatgaagtc ttctttgata aagtgttgcg
     7321 cttgatttga caattcaaga cataataaaa agcagatcaa atccaaataa gggaaaaatg
     7381 atctactctg gtattatcaa ttaattggcc aagctattat gccgttttgc taaacgtcta
     7441 cttcttccag tattgacctt gataaaacaa caatcaggat cccgttttaa tactctatga
     7501 actgtgtcaa tactaacatg taaagttgca gcaatatcag ctaatgtact cccatttaat
     7561 ctcatttgct ttgcaaatcg ataacgccga taagcatcaa tattcttgga atccatagac
     7621 aataatactt ttcttaccat gtatttcctc caaaaattga attttaatga gccatcattt
     7681 cttgagcaat ttcatgttta gacaatgaag ctggatagaa agtaggccaa ttatgcaatt
     7741 cattatagag tttttcttga ctttctccac caaagaaaat atggaaatgc tctgccttac
     7801 ttggtgcaat tccatgatca ctaaattgaa tatatttaaa acgaccagca cctgcatcat
     7861 cagtttcaaa attaaaacga acaccacggt ttcctttttc ataattcaaa attttgtatc
     7921 caacatattt gtatgtgtat tgatgatgtt caccgttgac tacaaaatca atggtgttgt
     7981 ctttaatgtt gatagtatca atatctgtct tatagccagt agtataataa tctttgtact
     8041 cggcagctgt catgtcttta ttgatttttg ctttgtaatc gaaaacttca tccaaaattc
     8101 catcttttaa caacggataa acagattgcc attcgcctgt ataatcactc aattcgcgat
     8161 ccttcacatc ggcatcttta aagtacccat tagcaactgt cttttcatcc ttcgcttctt
     8221 tttctggaac tacctcttca ccagcaacca tagttgtttt ttctaacgct tttagattgt
     8281 cttccattac tgaaacataa tcttcaccat tatccatttg ttcttttgta agactttcaa
     8341 gaggattcag tacttccaat tttatcccag cttcgttggc caaagttttt gcaattttat
     8401 cattagcatt cttttcaaag taaatataat tgatcttatt ttttttaaca tactctttta
     8461 attcagctaa tcgaccagaa gatggctctt cttcaggact tagtccagca ataggaactt
     8521 gtttcaatcc ataatccaaa gctaaatatc caaaggctgc gtgttgcgtc acaaaacttt
     8581 tttgtttcgc ttctttaaga ctagtggtat agtcagcatc caaccgtttc agtttagtca
     8641 aatacttttc agcatttgtc tcaaatactt ttgctttttt cggatacaat ttaactaact
     8701 gatccttgat atttgaaact tccttaattg ccattttggg tgaaacccac gtatgaggat
     8761 ctagttcatg atggtgatct tcttctccgt gatcatgatc ttcttcttca ctacctggca
     8821 ataaaatcat gtcttttgtt ccttctacta cattaggccc agctttcttc caactttctt
     8881 tagcttttgg tacccatgat tccatgtttt cgttatgata tacaaaaaca tcagcatcat
     8941 gagcttttgc catatcctta gctgagggct catagtcatg aggttctgat cccgctggaa
     9001 ccattaaatc cacgttcgct tcatcgccga cgatgttttt ggtgaaatca tacattggat
     9061 agaatgttgt tacaatttct aattttccat ctttatcatt cgatactttt gcatcattag
     9121 tgctggagca agctcccaat aatcctagag ctgctatccc aataagtcct acactgattt
     9181 tcttcattct ttttccttcc ttaattatgc taatttcttc attttatttc gtttatgttc
     9241 tttgataatt tttaatgccc tttttctagt tttaatattc gggcttttaa gtcgtcgata
     9301 tgcactagat atatccgttt tctccattag aaattacccc caatcactac ttatcattat
     9361 ttgctttccg ccagtaattt atcaaaactc tgtttgatcc tttcttcatc caacattttt
     9421 ccgattagaa taatagtggt ttggtctttt tgtcgagtgg tgtattgaaa attaacctgt
     9481 tcgtttacct cttgcatggc aactataaaa tcctgctctt gtaaagcaat caatccttta
     9541 aaacgataca ggctcccttg gttggttaaa atcagccagt caatccattg gagtaaaaaa
     9601 tcttttttta tgtcttgatc tgaagttaag agaattgatt taaactcatg tacttgatgg
     9661 tgttgatgat agtatttgcc attatagtga ttttctgatg aatcactttc aaaaactaag
     9721 ggatgattaa attttttcaa attataaaaa tccttttttt gaatcgtttc actatgtgtg
     9781 aacattcgaa tatcggataa agggttaata cttttgattt ctctctgtaa tttttttagt
     9841 gcagataaat tcaagttttg ttttctagac acaaaaatgc gatctgccat gacaagttgc
     9901 ttaagggctt gtggttctct ttcaataagt tcaaaactgt cactatcaat aacagtcaat
     9961 aaactatcga tatatactct tcctcttagt tgaggcgctg ttaaaagtgt ttgaatgatt
    10021 ggttggggat ctgctatacc tgtagtttca atgatgattt gtgaaattgg ttgttgatgt
    10081 tcttctattg tttccagtaa agaaatcaat attgctaata aatcactgcg aagactgcaa
    10141 cacatacatc cattatttag ttgtaacacc cgttcctcgc tatgaactaa taatttgtga
    10201 tcaatcccaa cttcaccaaa ctcattttca attataataa tttcttccct ggggtagggt
    10261 gagacttgca aggcttgatt gattagcgtt gtttttccag cccctaaaaa cccagaaacg
    10321 attgtaactg gtatgcccat tttgtcacct ttttaccact tagctatttc acttctttaa
    10381 aaattgcccg tctgcgcaat tttggcgaat acttttttaa ttctaaccgt tcagggttat
    10441 tccgtttatt tttgctggtg agatacagac gttctccagt ttcgacacat tccaaaataa
    10501 tattttgacg cataaatctt cctccttcaa tacttatcga tcttcaagat atgcttgaat
    10561 ttgttgtgca tcttcaaaag ttaactcgcc agattccaat tgtgaaacta aattttcttt
    10621 catatcaaca ggtatctccc aataaccatc gatacattga aataactcct cgtcatactc
    10681 ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat aagccttggt cctcatagta
    10741 atctttgact aatccatttt caattgcctg ttgggtcggt aaacccgttt cagtaataat
    10801 tcctctctct accatcgaac gataaaaagt gcatttatct agcgattgct gggtcgtttc
    10861 aagataatac tttaatcctt cttcaactaa aggtgggtaa ataatttcca ttttgattcc
    10921 ctcctaaaag ttatatatta aactattttg aagctccttt aattactttc tttccattat
    10981 agtacccttt taaagataca tgatggctac gtctataatc accattcgct tcgtcaaatg
    11041 aaatttctgg ttttactaca gtttgatgag ttcttcttaa ccgtttcttc gccttagatg
    11101 tttttcttgc tggtactgcc atgatatttc ctccttttac caacttgctt tttttactcc
    11161 aggaatcaac ccttgatggg caagttctct aaacttaata cgggacatac cgaactttcg
    11221 catataccct cttggacgac catccgtctg atcacgaagc ctcagacgat tcggatttga
    11281 atctcttggc aatttagcga gagcggtgcg atctcctgcg gcttttaatt cctgacgttt
    11341 atctgcatac ttttcaatca tcgccatttg tttctttgct ttagcaattt tcgacttttt
    11401 tgccatatac ctttatactc cttttatttt gatttagaca cctcaaatcg taatcgttac
    11461 gttttgacac tgatacttta ccaacaagag ttgctcttgt caacaataaa taacatgaaa
    11521 taaaactcat atgaatagat aactatttga aaacgaattt atcggtacat agagataatc
    11581 aaatcattta aaagaatatg cagtttaggc tctgaaaaat aagaattcac ttttattagt
    11641 ccaatcaaac acaaataccg ttttaagtag cttttcctct acttaaaacg gcaatatgaa
    11701 agaaactata actttttatt caatctttta tgtttaactg gtttatcgcc aattttttaa
    11761 aactttgatt attccttata ttataagaat agacattaag tgaactaatt caatcatact
    11821 gattctttcg tgtattcaaa attagatgct gtttaataaa attattcttt ttgaaaaaga
    11881 attataacta tatcccaaaa attgtaggtt aatttaaagc ctatttcatc atcgtgattg
    11941 agcttctttt tcatagacga atttctcaga tagtttagtt ttctttatga gatttttgta
    12001 tatttctgac cttcgataca actcattatg agtaccttga tcaactagtt ttccttgatc
    12061 catcactaca attttattta ccttttcaat agacttcaag cgatgcgata taattagtac
    12121 tgtcttatct tgtattaaac gatttaaact ttcctgaata cgtttttcat tttccgcatc
    12181 taaactggca gtaatttcat ctaaaattaa aattggcgca tttttcaaaa aagcccgtgc
    12241 tatggatagg cgttgtcgct ctcctccaga aagtgaactt ccgttttctc cgaccaatgt
    12301 atgataaccg tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac gagctgcttt
    12361 tttcacttcc tcatctgacg cttgactatt tccaatacgt atattttcca aaatacttgt
    12421 attaaacaaa gttacctctt gaaaaacaat agcgattttg ctaaacaaag aagctggaga
    12481 tacacgttta atatcatacc catcaattaa aatacagcct tcatcataat catacagtct
    12541 agacactaat ttcaagatac tagttttccc ggaaccacta gcacctacta acgcagtcac
    12601 ttctccttgt ttagccgtaa aagagatatg atctaaaatt ggggtattgt tatcatatga
    12661 gaaagagaca tctcgcaact caatgtcaaa agatttcaga ggactatctg atccctcttg
    12721 gatactggtt tccttcatag ctctaattcg ctgtatttta ggagctaaat aaaatatttc
    12781 aagcactgct tctttcatag aatcgaagga atcctttatt tttattgcgg ctagtaagta
    12841 accaacgacg tagagtatag tcacttcacc cgttaacagt aagtgaacac ctactagaat
    12901 gactactgct aaactaacat atgagaaaat agaggataag gccatgactg aaaaagtact
    12961 taattctacc tttaaatgaa tcctttcact ctcttccatt tttttgaaaa gtctgtcttg
    13021 aactttttta gatagattaa agctattaat ctcttgctgc aattctatag tttcttgaaa
    13081 ttcttccgag ttttctctca aagtatcgta atatttagta ttggctttcg tttgggattt
    13141 ctttgataac aagattaaga caaaactaaa taacgttggc aaaataacag ctaatcccat
    13201 tttgacatta ccaaccaaaa gcatcactga aataaaaggg aaaaacagag ccataccgcc
    13261 ggctttaggt attgcatgac tcatcgcatg ctcaatacct tcaacgtcag acatgatagt
    13321 ttgagataaa tctgataaat tatgttttga aaaataggat aatggtaaat ttgacaaatc
    13381 gtctgcaatt tgcactctta aatgcgcact ttctttataa gtactattat ataagttttc
    13441 atactcccta tctaacaacc aatacatgaa aagcaaagta acaaacgata cggcaaaata
    13501 aagccaacgg ggctttgtat tttctaaaac taattcgtct atcagcatca ttataatgaa
    13561 tgctggtacc atattaatgc agtatacaaa aaaactagaa atagaagctt tagttaaagc
    13621 ttctgcgccc tgatcactta aagcaaattt gtttttataa aaacttttca ttgtcaagcc
    13681 tccaatcatt tgcattctca taattagtga ccaattgttt atacacttga tgtttagcca
    13741 ttaattcatt atgatttcca cgttctacta ttttcccttc tgacaagaca agaatttcat
    13801 ctacagattt aatgctggat aatcgatgag ctatcataat aactgttttt tctttcatca
    13861 gatgttgaaa agccttttgt agttcatact cattatctgg atcaatagat gcactggctt
    13921 catccataat aacaatcttt gcatctttta aaattgctct ggcaatagct atacgttgtt
    13981 tttctcctcc tgacagataa actcctttac taccaattaa ggtatgctct ctttcgggaa
    14041 atttttctaa aatcgattcg caaccggcta atcttagcgc gtcaaatact ttacttgctg
    14101 gcgcttgagg atttgctata gaaacgttat cgtaaatact ctgattgaat agtttcgtat
    14161 cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga tttaaaggct
    14221 taccaccaat tttaatctgt cccttatcca cattgtagaa acctgaaatt agtttagcta
    14281 tcgtactctt tccgcttcct gattttccta ctaatgcata aattcttttt tgttctaaaa
    14341 ctaagttgaa attttctaaa acacactctt ctttgtatga aaagctgaca ttttcaaatg
    14401 caatgtcaca atttttaatc tgtgtctcgt tcccatattt tagttggtct tgcttcattt
    14461 ctaaataaag ggagtctagt gtttcgactg catatcttgc ttcaaacaag tgcatactta
    14521 catacattat tctcatgaaa gaaacaaaaa taattccact taaaaaaaac aacatcaaca
    14581 agtcaacagc taatatcttg ggagtggtaa gagaagttat aaaaaaggtt ataggaacta
    14641 tcaaaatgca aactatgcca aaaaatagcc attgaaaaag aacaaaaggt gttttcccac
    14701 tcaaagaata cttatatgca aacttagcat aatcgttaat tgctttattc aaggctttaa
    14761 atgatgttac tttagcacca aatatcttta taacttgaat tccgcggata tattctaccg
    14821 tttcagagct taacacatct aacgattctt gatatttttg aataaaattt gtattctctg
    14881 tcattttctt taacaagaat acaccaatta ctaacatggc gattagaacc aatccaattt
    14941 taaagttaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg gcttgagcac
    15001 tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca tcaattgtct
    15061 ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct gttaaaccgc
    15121 taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga gaaaataagc
    15181 cagaaaaaat ataaaataaa gctctaaagt taaccaagca acagtttgaa ccgcataact
    15241 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    15301 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    15361 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    15421 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    15481 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    15541 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    15601 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    15661 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    15721 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    15781 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    15841 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    15901 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    15961 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    16021 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    16081 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    16141 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    16201 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    16261 cttttctttt aagtctttaa taaggtcagc aatcctttc
//
LOCUS       DASJOX010000053        16097 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJOX010000053 DASJOX010000000
VERSION     DASJOX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.054"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4A12_001631"
     CDS             226..894
                     /locus_tag="U4A12_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN1773988.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="U4A12_001632"
     CDS             complement(1094..1480)
                     /locus_tag="U4A12_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773989.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="U4A12_001633"
     CDS             complement(1452..2834)
                     /locus_tag="U4A12_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1773990.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="U4A12_001634"
     CDS             complement(2990..4177)
                     /locus_tag="U4A12_001634"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN1773991.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="U4A12_001635"
     CDS             complement(4184..5215)
                     /locus_tag="U4A12_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1773992.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="U4A12_001636"
     CDS             complement(5231..6685)
                     /locus_tag="U4A12_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1773993.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="U4A12_001637"
     CDS             6860..7765
                     /locus_tag="U4A12_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1773994.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="U4A12_001638"
     CDS             7856..8533
                     /locus_tag="U4A12_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1773995.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="U4A12_001639"
     CDS             8530..8700
                     /locus_tag="U4A12_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1773996.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="U4A12_001640"
     CDS             8814..9710
                     /locus_tag="U4A12_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1773997.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="U4A12_001641"
     CDS             complement(9765..11135)
                     /locus_tag="U4A12_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1773998.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="U4A12_001642"
     CDS             11373..12119
                     /locus_tag="U4A12_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1773999.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="U4A12_001643"
     CDS             12116..12955
                     /locus_tag="U4A12_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1774000.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="U4A12_001644"
     CDS             12956..13906
                     /locus_tag="U4A12_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1774001.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="U4A12_001645"
     CDS             14047..14799
                     /locus_tag="U4A12_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774002.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="U4A12_001646"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="U4A12_001646"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN1774003.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5220 a         3009 c         3233 g         4635 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatg
//
LOCUS       DASJOX010000054        16078 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJOX010000054 DASJOX010000000
VERSION     DASJOX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.055"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            164..1309
                     /locus_tag="U4A12_001647"
     CDS             164..1309
                     /locus_tag="U4A12_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1774004.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="U4A12_001648"
     CDS             1446..1790
                     /locus_tag="U4A12_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN1774005.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="U4A12_001649"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="U4A12_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN1774006.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="U4A12_001650"
     CDS             complement(3485..4084)
                     /locus_tag="U4A12_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN1774007.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="U4A12_001651"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="U4A12_001651"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN1774008.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="U4A12_001652"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="U4A12_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN1774009.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="U4A12_001653"
     CDS             7290..8408
                     /locus_tag="U4A12_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN1774010.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="U4A12_001654"
     CDS             complement(8468..9760)
                     /locus_tag="U4A12_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1774011.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="U4A12_001655"
     CDS             9892..10470
                     /locus_tag="U4A12_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1774012.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="U4A12_001656"
     CDS             10599..10976
                     /locus_tag="U4A12_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN1774013.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="U4A12_001657"
     CDS             10909..12981
                     /locus_tag="U4A12_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1774014.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="U4A12_001658"
     CDS             complement(13192..14139)
                     /locus_tag="U4A12_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1774015.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="U4A12_001659"
     CDS             14385..14849
                     /locus_tag="U4A12_001659"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN1774016.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="U4A12_001660"
     CDS             complement(14911..15954)
                     /locus_tag="U4A12_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN1774017.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         5255 a         2931 c         3388 g         4504 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaata
//
LOCUS       DASJOX010000055        16058 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJOX010000055 DASJOX010000000
VERSION     DASJOX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.056"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(407..766)
                     /locus_tag="U4A12_001661"
     CDS             complement(407..766)
                     /locus_tag="U4A12_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN1774018.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="U4A12_001662"
     CDS             complement(796..1137)
                     /locus_tag="U4A12_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774019.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="U4A12_001663"
     CDS             complement(1134..1808)
                     /locus_tag="U4A12_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1774020.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="U4A12_001664"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="U4A12_001664"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN1774021.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="U4A12_001665"
     CDS             complement(4322..5512)
                     /locus_tag="U4A12_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1774022.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="U4A12_001666"
     CDS             complement(5533..6060)
                     /locus_tag="U4A12_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN1774023.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="U4A12_001667"
     CDS             complement(6107..8896)
                     /locus_tag="U4A12_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1774024.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="U4A12_001668"
     CDS             complement(9043..9243)
                     /locus_tag="U4A12_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1774025.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="U4A12_001669"
     CDS             complement(9696..10016)
                     /locus_tag="U4A12_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774026.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10231)
                     /locus_tag="U4A12_001670"
     CDS             complement(10061..10231)
                     /locus_tag="U4A12_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774027.1"
                     /translation="MTKIISQIFVKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKN
                     IIYEQNVNNGRS"
     gene            complement(10519..11637)
                     /locus_tag="U4A12_001671"
     CDS             complement(10519..11637)
                     /locus_tag="U4A12_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774028.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="U4A12_001672"
     CDS             12108..13415
                     /locus_tag="U4A12_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1774029.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="U4A12_001673"
     CDS             13536..14735
                     /locus_tag="U4A12_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1774030.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="U4A12_001674"
     CDS             complement(14762..16030)
                     /locus_tag="U4A12_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN1774031.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
BASE COUNT         4607 a         3428 c         2857 g         5166 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacg
//
LOCUS       DASJOX010000056        15741 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJOX010000056 DASJOX010000000
VERSION     DASJOX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.057"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..1438
                     /locus_tag="U4A12_001675"
     CDS             11..1438
                     /locus_tag="U4A12_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774032.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1530..1895)
                     /locus_tag="U4A12_001676"
     CDS             complement(1530..1895)
                     /locus_tag="U4A12_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1774033.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1892..2257)
                     /locus_tag="U4A12_001677"
     CDS             complement(1892..2257)
                     /locus_tag="U4A12_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1774034.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2449..2697)
                     /locus_tag="U4A12_001678"
     CDS             complement(2449..2697)
                     /locus_tag="U4A12_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774035.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2696..2824
                     /locus_tag="U4A12_001679"
     CDS             2696..2824
                     /locus_tag="U4A12_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN1774036.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2881..4575
                     /locus_tag="U4A12_001680"
     CDS             2881..4575
                     /locus_tag="U4A12_001680"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN1774037.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4579..4698
                     /locus_tag="U4A12_001681"
                     /pseudo
     CDS             4579..4698
                     /locus_tag="U4A12_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5226..6359
                     /locus_tag="U4A12_001682"
     CDS             5226..6359
                     /locus_tag="U4A12_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN1774038.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6390..6710
                     /locus_tag="U4A12_001683"
     CDS             6390..6710
                     /locus_tag="U4A12_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN1774039.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6739..7203)
                     /locus_tag="U4A12_001684"
     CDS             complement(6739..7203)
                     /locus_tag="U4A12_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774040.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7335..8588)
                     /locus_tag="U4A12_001685"
     CDS             complement(7335..8588)
                     /locus_tag="U4A12_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1774041.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8617..8934)
                     /locus_tag="U4A12_001686"
     CDS             complement(8617..8934)
                     /locus_tag="U4A12_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1774042.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8960..9280)
                     /locus_tag="U4A12_001687"
     CDS             complement(8960..9280)
                     /locus_tag="U4A12_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774043.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9744..11159)
                     /locus_tag="U4A12_001688"
     CDS             complement(9744..11159)
                     /locus_tag="U4A12_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1774044.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11176..12492)
                     /locus_tag="U4A12_001689"
     CDS             complement(11176..12492)
                     /locus_tag="U4A12_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1774045.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12642..13340)
                     /locus_tag="U4A12_001690"
     CDS             complement(12642..13340)
                     /locus_tag="U4A12_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN1774046.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13629..14585)
                     /locus_tag="U4A12_001691"
     CDS             complement(13629..14585)
                     /locus_tag="U4A12_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1774047.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14698..15531)
                     /gene="msrA"
                     /locus_tag="U4A12_001692"
     CDS             complement(14698..15531)
                     /gene="msrA"
                     /locus_tag="U4A12_001692"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1774048.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4899 a         3185 c         2688 g         4969 t
ORIGIN      
        1 aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc tgtttttatg
       61 tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt
      121 ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg aagaacagat
      181 cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac atatacctcg
      241 atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta
      301 tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca tctctgtcag
      361 caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg ggatcgaaat
      421 cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc
      481 gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg aacgtgccga
      541 tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag aaaataaaga
      601 aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg ctgtgacaat
      661 agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc
      721 atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct ttattttgac
      781 actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg aagactttca
      841 acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag ttgctacctt
      901 tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg cgctgattca
      961 tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat tttatctttt
     1021 aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca gctggtccag
     1081 ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg tgatcgaatt
     1141 gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca caaattcgga
     1201 tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg attttcaagt
     1261 tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc agtcctcaaa
     1321 ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg aaaatattat
     1381 tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa acaaataagg
     1441 ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat ttctgtaaca
     1501 atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag gggtccgatc
     1561 tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc tagttggaaa
     1621 atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac aagaatttcc
     1681 aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc gcttactcgg
     1741 tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat tgatagacaa
     1801 acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag tttttcgaaa
     1861 tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt ctttgttttt
     1921 ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg ataataataa
     1981 gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg atatcagcaa
     2041 cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt gtatctgcat
     2101 tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa agtttgaata
     2161 gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa atagcaatga
     2221 tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac tttcttttaa
     2281 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
     2341 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
     2401 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
     2461 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
     2521 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
     2581 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
     2641 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
     2701 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
     2761 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
     2821 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
     2881 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
     2941 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
     3001 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
     3061 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
     3121 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
     3181 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
     3241 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
     3301 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
     3361 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
     3421 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
     3481 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
     3541 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
     3601 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
     3661 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
     3721 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
     3781 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
     3841 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
     3901 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
     3961 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
     4021 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat
     4081 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
     4141 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
     4201 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
     4261 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
     4321 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
     4381 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
     4441 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
     4501 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
     4561 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     4621 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
     4681 gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga
     4741 gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg
     4801 caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga
     4861 agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga
     4921 aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact
     4981 attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac aactatttca
     5041 atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac
     5101 tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt
     5161 aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat
     5221 ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg
     5281 atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg
     5341 gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag
     5401 gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag
     5461 ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc
     5521 acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat
     5581 ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg
     5641 cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc
     5701 tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg
     5761 atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta
     5821 tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg
     5881 ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat
     5941 tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat
     6001 tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc
     6061 cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt
     6121 atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca
     6181 agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag
     6241 gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag
     6301 catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa
     6361 ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga
     6421 tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc
     6481 aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt
     6541 ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg
     6601 ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt
     6661 acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca
     6721 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
     6781 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
     6841 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
     6901 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
     6961 atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac
     7021 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
     7081 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
     7141 attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
     7201 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
     7261 aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc
     7321 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
     7381 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
     7441 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
     7501 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
     7561 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
     7621 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
     7681 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
     7741 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
     7801 ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
     7861 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
     7921 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
     7981 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
     8041 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
     8101 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
     8161 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
     8221 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
     8281 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
     8341 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
     8401 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
     8461 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
     8521 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
     8581 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
     8641 attaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
     8701 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
     8761 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
     8821 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
     8881 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
     8941 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
     9001 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
     9061 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
     9121 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
     9181 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
     9241 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
     9301 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
     9361 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
     9421 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
     9481 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
     9541 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
     9601 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
     9661 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
     9721 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
     9781 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
     9841 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
     9901 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
     9961 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
    10021 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
    10081 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
    10141 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
    10201 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
    10261 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
    10321 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
    10381 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
    10441 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
    10501 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
    10561 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
    10621 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
    10681 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
    10741 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
    10801 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
    10861 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
    10921 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
    10981 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
    11041 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
    11101 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
    11161 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
    11221 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
    11281 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
    11341 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
    11401 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
    11461 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
    11521 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
    11581 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
    11641 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
    11701 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
    11761 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
    11821 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
    11881 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
    11941 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
    12001 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
    12061 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
    12121 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
    12181 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
    12241 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    12301 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    12361 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    12421 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    12481 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    12541 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    12601 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    12661 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    12721 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    12781 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    12841 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    12901 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    12961 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    13021 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    13081 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    13141 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    13201 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    13261 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    13321 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    13381 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    13441 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa attcactcct
    13501 agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa cagacgatct
    13561 tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa agtttgatta
    13621 catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga gaaatctaaa
    13681 ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc gaccagtaca
    13741 cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa tgattgtcct
    13801 cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc aacttgttct
    13861 tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat accatgtaga
    13921 taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc tagcccttct
    13981 tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat attgtagcgc
    14041 atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat tttttttgta
    14101 tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct tacaagttgc
    14161 atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg atgatagaag
    14221 atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga aatgatggat
    14281 ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc tttgctagaa
    14341 ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc
    14401 tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt aattcccatg
    14461 tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc accaaaattt
    14521 tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga tttacgatat
    14581 tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc tcatcaaatc
    14641 tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga aaaggagtta
    14701 atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt gatagatcag
    14761 aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt tgtaaaattg
    14821 ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg tgacgattgg
    14881 ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta attctttttg
    14941 tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt gaaattgtcc
    15001 aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc tataacttat
    15061 ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac caccacttac
    15121 ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa cagataggat
    15181 cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg caaatactgc
    15241 acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt tttgctcctt
    15301 atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca gattcattcg
    15361 gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa cggatatatt
    15421 ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg
    15481 attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca tagattctcc
    15541 tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa gtgaggaaat
    15601 aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat ccttatcgac
    15661 agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttaac ttttatggaa
    15721 aactatttta aaaaataaac a
//
LOCUS       DASJOX010000057        15673 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJOX010000057 DASJOX010000000
VERSION     DASJOX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.058"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..1535)
                     /locus_tag="U4A12_001693"
     CDS             complement(12..1535)
                     /locus_tag="U4A12_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1774049.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="U4A12_001694"
     CDS             1787..2968
                     /locus_tag="U4A12_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1774050.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="U4A12_001695"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="U4A12_001695"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN1774051.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="U4A12_001696"
     CDS             complement(5053..7764)
                     /locus_tag="U4A12_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774052.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="U4A12_001697"
     CDS             complement(7774..8475)
                     /locus_tag="U4A12_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774053.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="U4A12_001698"
     CDS             8661..9260
                     /locus_tag="U4A12_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1774054.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="U4A12_001699"
     CDS             9341..10354
                     /locus_tag="U4A12_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1774055.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="U4A12_001700"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="U4A12_001700"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN1774056.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="U4A12_001701"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="U4A12_001701"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN1774057.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="U4A12_001702"
     CDS             11802..13175
                     /locus_tag="U4A12_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1774058.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..>15673
                     /locus_tag="U4A12_001703"
     CDS             13532..>15673
                     /locus_tag="U4A12_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1774059.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         4606 a         3133 c         2911 g         5023 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaa
//
LOCUS       DASJOX010000058        15589 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJOX010000058 DASJOX010000000
VERSION     DASJOX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.059"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..738)
                     /locus_tag="U4A12_001704"
     CDS             complement(25..738)
                     /locus_tag="U4A12_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1774060.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            913..2337
                     /locus_tag="U4A12_001705"
     CDS             913..2337
                     /locus_tag="U4A12_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1774061.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2636..3343
                     /locus_tag="U4A12_001706"
     CDS             2636..3343
                     /locus_tag="U4A12_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1774062.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3330..3653
                     /locus_tag="U4A12_001707"
     CDS             3330..3653
                     /locus_tag="U4A12_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1774063.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3742..4779)
                     /locus_tag="U4A12_001708"
     CDS             complement(3742..4779)
                     /locus_tag="U4A12_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1774064.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5100..6083)
                     /locus_tag="U4A12_001709"
     CDS             complement(5100..6083)
                     /locus_tag="U4A12_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN1774065.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6498..7268)
                     /locus_tag="U4A12_001710"
     CDS             complement(6498..7268)
                     /locus_tag="U4A12_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774066.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7261..8196)
                     /locus_tag="U4A12_001711"
     CDS             complement(7261..8196)
                     /locus_tag="U4A12_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774067.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8512..10161)
                     /locus_tag="U4A12_001712"
     CDS             complement(8512..10161)
                     /locus_tag="U4A12_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1774068.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10278..12881)
                     /locus_tag="U4A12_001713"
     CDS             complement(10278..12881)
                     /locus_tag="U4A12_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1774069.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12893..13942)
                     /locus_tag="U4A12_001714"
     CDS             complement(12893..13942)
                     /locus_tag="U4A12_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN1774070.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13968..14282)
                     /locus_tag="U4A12_001715"
     CDS             complement(13968..14282)
                     /locus_tag="U4A12_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN1774071.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14298..14744)
                     /locus_tag="U4A12_001716"
     CDS             complement(14298..14744)
                     /locus_tag="U4A12_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1774072.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14745..>15589)
                     /locus_tag="U4A12_001717"
     CDS             complement(14745..>15589)
                     /locus_tag="U4A12_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1774073.1"
                     /translation="FKRSFMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKP
                     DKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPI
                     ENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNV
                     LNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPE
                     GIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLKTSSSFEKLVYIL
                     KEGL"
BASE COUNT         4624 a         3211 c         2593 g         5161 t
ORIGIN      
        1 tctgaaaaaa taattttccc gagcttattt ttcaacaaaa acagcttcaa aaacaaagtc
       61 ttgatataaa tgtctcgata ccgaatactc gaaaggttta ccagacgata gagcaacaac
      121 tttgtctatc tcaatcataa attcacctgg atctattttt aatatttttg cgtcaaattc
      181 attcgcagta tctcctttga tacgtatgtg cgaagtcttt atttccagac ccagttcatt
      241 tgtgatataa gcatatacag atttctttaa atgttttggt tccaagcctg gtatgactgc
      301 taatggcatg aatgtttgct caatcgagta tggttgacaa tcaataaaac ggcttcgtac
      361 aatattatat acatagtctt ttacactgat acttaattta tcagctactt ttttcgatgg
      421 aagttcaatt gagaaagtat ggatttctga atctacgtgt tcttcaccaa ccacgctaaa
      481 caaaccttcg tttggaccaa gaactcgtga gttatcaatt ggtgttctga cgacttcagt
      541 cccataccca cttctacttc gcaaaactcc ttcggaaaca agtaagtcta cgcctttctt
      601 tactgttaac ttgcttgtat gataaataac tgccaattca tctcctgtag gaagtttctc
      661 accaatttta tactctccat ttacaatttt tttctttata tctaagtaaa tttccatata
      721 cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac atatcttcat ctatacttta
      781 acataaataa accgataaaa tccacatcaa aattatacac acaataaaaa agatatatat
      841 atttaaacaa aactatttac tttaagttgt aagcggtgtt atcattaagt ggaaacagga
      901 ggtaaaaaaa ttatgacaac tatgaaaaag ctaacttttc cagatgattt ttggtggggt
      961 tccgcttggt cagctgagca agcagaagga aaaggagaaa caggaaaagc agagactatc
     1021 tgggatcgct ggtttaaaga acaaccttac cggttttatc aacgaatcga ttcctctatt
     1081 acaaccgatc acatccatca atataaagaa gatatagaac taatgaaaaa aactgggcat
     1141 aactcctttc gcgtttctat ttcttgggca cgaatgtttc caaatggcgg taccggaaaa
     1201 gtaaatccca aagccatttc tttctatcga aaattatttt ccgaaatgaa taataacgga
     1261 atcaaagtat ttgccaactt atatcatttt gacatgcctg ctactcttca agataaagga
     1321 ggatgggaat cacgtgaagt agttgatgct tatgttcatt acgctgaaac ttgttttaaa
     1381 gaatttggtg acttagtttg tcattggttt accttcaatg aaccacttgg gcctatttta
     1441 ggtacttatc ttgaagattt tcattatcca aacctcattg actttaaacg cggagcgcaa
     1501 gcagcattct tcactatact tgcacacgca aaagcaatac aagtatttaa agaactagct
     1561 cttcctagta aaataggcgt tattctaaat ttgagtccta cttttcctcg tagtcaaaat
     1621 cctgctgatg tgaaagcagc agaaatcgct gatttatttt atacgcggag ctttttagat
     1681 cctatggtta aagggaattt ttctaaaaag cttatcctgt tattacaaga atataatcag
     1741 cttcctgaag attatacgaa agaagatctt gtatgtatct acgaaaatac cgctcaaatt
     1801 cttggactaa actattacga gccacgtcgc gtcaaatctc gttcttctat ggtggataca
     1861 tctagtccat ttttaccaga gtggtttttc gaaccacata tcatgcctgg acgacgaatg
     1921 aacgaatacc ggggatggga aatttacgaa aaaggaattt atgatttgtg catggatatc
     1981 aagaaccatt atggaaacat cgaagcattt atctcggaga acggaatggg cgttgcggat
     2041 gaagaacgtt tcatggatga tacagggcaa ataattgatg attatcggat tgagtatatc
     2101 aaagatcatc ttgcttactt atggaaggca ataaatgaag gatgtaacat taaggggtat
     2161 catctttgga catttattga ttgctggtca tggatcaacg cctataaaaa ccgatatggg
     2221 ctggtttcac tcgaccttac tactcaaaaa agaacaatta aaaaaagtgg cgaattttac
     2281 aagcaattaa gtgatacaaa tggatttaga tacgatgtag acttgttagt tgaataatat
     2341 tctaaaattc cacttgaagt gaacacatta gaaaacaaga ataatgagaa ataccaataa
     2401 caaatacttt ttcttcatac ttcatttata cttgtcagta taagagactg tgacaaatgt
     2461 agtgatcctc agaagttagg tctggaaatc taacttgaga agattttttt aaactacgtc
     2521 cagtcttttt tatttgttta aaattgaagt tgacaagtct tcattaaaaa tttagaatat
     2581 tcacaagttt gacgacaatt atgaaaattt atgtcgtgct aaaggagttt tgttattgga
     2641 tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta cctaccgttt ttggttatat
     2701 cggtattgga cttgcatttg gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt
     2761 cacgttgatg tccctgctga tctatgctgg ttctgcccaa tttatcacgg tcagcatgct
     2821 tgctagccat agtccactgc tctccatcgt tctctcgacc tttctagtca attcccgaat
     2881 gattctgatg agtatgacga ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct
     2941 gctgattggc acattgctga cagatgaaag tttcgcactg ggaatgaata aattgaacta
     3001 tactggacaa aagttaaact ttcgttggat gaatacagct aattggattt cttatttgac
     3061 gtgggtgggt tcttctcttg ttggcgcact tttagggaat tttattactg atccgaaaaa
     3121 gtttggtttg gatttcgcca tcgtcgctat gtttatcggg ttactttatc ttcaagtcat
     3181 ttctgataga aacacctcta aacgattaca gttgatcctg attggtctca ctttgattct
     3241 ggtatatgtc ggactgattt ttatcccaag taacttagtg attttagttg taacactgat
     3301 tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt tgagtttaca ttgctgacta
     3361 ttttaggttg tactttagca acttggcttt ctaggatcct accatttgtc ttactgaaaa
     3421 aattcgattt gccgcaaccg ttgcttgagt atttaagttt tgtcccgatc gtcattatgt
     3481 ctgctttatg gttcagcagt ctatttaccc aaaatatcgg acatctgccg cagatcaatc
     3541 tcgaaaatgc tttggcttca gttcctacac ttttagctgc tattctttca aaaagcttat
     3601 tggtgatcgt cttggtagga attctttccc tttcgttgat tcgcttatta taaataaaaa
     3661 aacaagagct tatggcaaat tactgtcatg cgctcttgtt tttatcaaag tcaaataaga
     3721 aaaaaatcgg cgtattcttg cttatccgtt gaacggacca ctaacaacga cttttcctaa
     3781 tatatgtcct gtttctacgt ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc
     3841 tgaatagacc ttgctcaatg tactgcggat aatcccttca tttgctaaag cagcaatgtg
     3901 cgctaaagct tctccttgcg tttcgatttc atagttatag tctgttttag cgaacatata
     3961 ctcccagtcg aagctgatag attggttttt ccaaacacct atatctaacg gctctttgat
     4021 ccctacgatc gttcccacat gtccaaatgg tcggacaagg tgtttgattt gatcaaagta
     4081 gcgggtaata tcgaataata cagcgacata ctctacttga tcgattccta attcacgaaa
     4141 acttttactt aagtcgtgat gataatcgat tggatagtct acacctgttt ttttcagcca
     4201 ttcaaaattc ttcggacttg cagtcgcgta gacttctagg ccagcccaat gcgctagttg
     4261 attcaaaatc gaaccgacgc ccccagctcc gttgatcact aagatttttt tcccgcggtt
     4321 ggcattttct tctggagtca gaccaaattt ttcaaataac aattcataag cggtcaatga
     4381 ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt tttggtgcaa gagcgacaat
     4441 acgttcgtca accagttggt attcttgatt gcttcctgct cttgtcgttg tacctgcgta
     4501 aaaaacgcga tcgccgacag aaaatttttg cacttcatca cctacagcaa caactttccc
     4561 gactccatca aaccctaaga tacgtaaagc atttcttatc ccgttatctt ggcgaagttt
     4621 cgtatcgaca ggattaacag atactgcgtt gacttttacc agaagatccc gtgaactggg
     4681 tacaggtatc tgttcttttt catcaataaa gctttttgga tcatcgatcg acaacccttc
     4741 ataaaaaccg actaccttca ttaattcctt ttctgacata cgaattcctc cttagcttca
     4801 tcaagttgga gcatccatac agttttcatt tcaatatata tagtatgcta tttgttccac
     4861 ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt tttggttaaa
     4921 aaagaggttg cgacaagact tttgtcacaa cctcttttaa acggtgttca atcaactgac
     4981 cctcggtatt cgctcaaaaa acgacaaaat atgaggagct atttttgttt ggctgttctt
     5041 attacttgat gcaggacagc tttttcacga cctctaattc acggtgttca agcaagaaat
     5101 cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tgtctaagat ctctcgtact
     5161 tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc tggaaatcct
     5221 ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat gatttctgaa
     5281 taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt ttctgtatgt
     5341 tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa tcccccggcc aatcatgatg
     5401 ccatctacgc catatttttc ggccaattct tctcctgtct ggcgatctgg gatatctccg
     5461 ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta aagcaatgat tttagggatg
     5521 acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg gacagaaaga
     5581 ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga catttccgta
     5641 tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagccgc ttcaatcaat
     5701 tgtgccgcta cttctgggcg aaggatcaag ccactgcctt ttccacgttc tgctacatta
     5761 gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc catagcaatg
     5821 ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat cggctgttca
     5881 tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc catctggatg acagaaactg
     5941 tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt tacgacatgg
     6001 cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc
     6061 tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag cagtgaagtc
     6121 gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag caaagtgaac
     6181 agcttaaaag atgtttctga ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt
     6241 ccttttccgg aataaatggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac
     6301 gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt
     6361 ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa
     6421 cgacaaaata tgaggaacta ttttcccgtt tgtttctcta atactcaggt caaaacgctg
     6481 atttcacaac ctcttttcta acttctcaat cctcttcctt tattgatcaa ataccaagta
     6541 acggcgtaca aaacaacgat gaatagcaca agtacgatca tcgatgttgc tactggcaca
     6601 tcgattgttc caaggaatcc gtaacggaaa ccggagatca tataaacgat cggattgact
     6661 ttcgaaacag cttgccaaaa tggcggcaac atagagatgg catagaaaac gccacctaga
     6721 taagttaacg gctgcaagac aaatgtaggc acgatcgata catcgtcata agactgagca
     6781 aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg
     6841 atgaccatga accatgaata cacatgaagt ggtacaaaga acaaggaaat gatcgttacg
     6901 agagagccga cgagaatact gcgacccact ccgccaatca caaagcccca aatgatcaca
     6961 tgcgtaggaa caggcgctac caagatttct tcgatgtttt tctggaattt ttgagagaag
     7021 aaggacgaag aaacgttggc atacgaacta gtgatagcag acatcataat caaaccaggc
     7081 acgatgaatt ccatatatga aaaaccaccc atgtcaccga tccgaccacc gatcattttt
     7141 ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag
     7201 atacgcaaat agcggttcgt ttccttgata gctaaacttt ttaatgcagt aaaataaaga
     7261 ttaaacattt ttctcctccg tatgattatt ttcttctgtg attttcaaaa acagttcttc
     7321 taaacgattc gacttattcc tcatagaaag aactttgatt ccttgtcttg tcagctgatc
     7381 gaagattccg ttcacccctt gatttcgttc aacttcgaca gcgattgtct gttcatcttc
     7441 aaatgtacaa gcgtatcctt caatcacagg tttggtctca tatggcgcta aatcaaagat
     7501 aaatgtttca aattgcagtt tggatagaag ctctttcata ctagtattct cgatcagttc
     7561 acctgattgg atgatcccga tgttgcgaca aagcatttca gcttcttcta aataatgcgt
     7621 cgtcaaaatg atcgttgtac cattttcgtt caattctcgg agaaactccc acatctcgcg
     7681 tctcaattca atatcgactc ctgctgttgg ttcatccaag atcagcaaac gtggttcatg
     7741 catcaatgcc ctagcaatca tcagtcttcg cttcattccc ccggaaagca tacgtgcacg
     7801 gacatttctc ttttcccaaa gattggactg cttcaaatat ttttcgctgc gtttcatcgc
     7861 ttctttgcga gagacaccgt agtagcctgc ttgattcacg acgatttgct ggaccgtttc
     7921 aaagggattg aaattgaact cttgcgggac taatccaatc tgttgctttg ctcggactag
     7981 atccgtatcc agatcataac cgaagacatt cacttttcca gatgttttat tgactaaaga
     8041 agtaatgatt ccgatcgttg tagacttccc agctccattt ggtcctaata gcgcatagaa
     8101 atctccttct ttcacggaaa gatcgattcc tcgcaatgct tcgaccccac ttccataaac
     8161 ctttttcaaa ttatttattt caagcgcatt tgccatttat ttcctatctc cttatagaca
     8221 tgattcttta ttgtatcatg gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa
     8281 atcaggttgc tgggctcagg aaaatctcct aatgaaagac atcaatcgga cgaagtcaga
     8341 caaccttttc agaaaaaaca aatcgaagat aaaggattac tattcatatg tgcaacaaaa
     8401 ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     8461 ggaagcaaaa gccaagattt ttgatccaat aaatactttc gatcacttcg cctatcccaa
     8521 tacatgccac tgccgctttt catcaaataa cgcggcttcc gtttttgcgg tttctactaa
     8581 agtctctggg taatccaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc
     8641 ttcttttaat ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg
     8701 gacattctca caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa
     8761 tgctaaagat ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc
     8821 gatcgtattt gtccctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc
     8881 cacttggtcc aacaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc
     8941 aaataaatca tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg
     9001 ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac
     9061 atacgtagat tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac
     9121 cggattcact accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc
     9181 ctcagtaaac tctggtcggc aagtgatggg catataagta cggtaattca atattgcagc
     9241 ggctacttct aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc
     9301 taatttagtg agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata
     9361 ttcaaaaatc aactctgctt cactcccact cttcatacga aatgaaaaac ctaatttagg
     9421 gatgtctctt aatggtttag tttgctgttt cagctcttct tgaagtggtg tcggcagttt
     9481 tgctagctga ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt
     9541 ttctagtgtt tgtttcttca ctagataata gaccgtattg aatactaacc caccgattgc
     9601 caaatagatt cctagtactt gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa
     9661 gccaagaaaa ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata
     9721 ttgttttgca aaattatgat ctttttttcc taaacgagcg aattgatatt ggattttttc
     9781 tctcagttga ggattttcct gataaaaagc aatcagtttt tctaactgct gatcttcacc
     9841 aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa cctacactag aataggtact
     9901 gttgattcct tcgaatactt cgaacatatc caggtcatac caagtaagat catcgatttc
     9961 gctatcccat gaacggaact tcttctccgt gagccatgct tccttcaagc tttcttcttt
    10021 atcaaatctc ggttgataag gaattttttc ccatttgcta cgaactgttt cctttaattt
    10081 caaacgtgaa aaaatgtcta aagcaactaa taaaataaca gctgcaataa taattaaaac
    10141 gataaattct ctttgaccca tatccctctc cgttccttta tttgttagct ttagtatagc
    10201 actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt
    10261 tcttgtatat tacatattta tctctcattt atcaaaattg acaaataatc atagggagga
    10321 attgttgtta attcttgttt attctccagt gcattgacaa ctgtcccaag ggaaaggata
    10381 gcctcaggta ctattttttc ttgttctgtt ggattagcta agcgaagaac aaatccttgt
    10441 tcatgaaaat aagaaggata aatcgatgat acaattaagt cttcaggtaa ttcaatcaat
    10501 gaaaatttac gaggaacatc aagttcattt tgtaatggcc aaatcttgtt atctaacctg
    10561 tgaataaata cattcagttt ctgcttttga taagaaatag attgttctaa ccgtttttct
    10621 gcctgttttg ccactttgaa ctcattaaat cttccttctt ctacattaaa tccaaaagtg
    10681 actttatact cacctatctc ttgagctagc ggtgtttcca tcatgacatg cccttcattt
    10741 gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat taggttttcc aagttcccct
    10801 gttgttgaaa ataaggttat gattaattga ttttctatac gttcatactc ttttaaacca
    10861 tctgcataaa acgtaaaata atgatcattt tctgccacag agacactctt atcgaatatt
    10921 tctaaattaa caggtttctc taccatagtt tctgaccaat tcggaggaat gacatcaggg
    10981 gtattttcaa taaatccgtt ttgaatttgt gcaattgatt tttcagattt atctaaaaca
    11041 ttaattgcta agcgcatacg atgcgagtaa acagtattat gaattttgag tgtgccttca
    11101 acgatttgac tttctcccag actgaacgat atttcataag atatttcttc catttcgggt
    11161 ttttcagata aacgatcttt taaatcttta ggaagttgag cttttccgta aacaactaat
    11221 gtttcttgta aagaatcttt atatacttta gctgtttcta attttagaga tagttctgta
    11281 tctccttcta atggtgaata atcatacgta tcgccatcat ttgctgaatc gatcaaatga
    11341 acgaaatcaa gatattgtct atctccaact atcaaattca attgcccatc tttaaaagaa
    11401 agtttatatt tattgttact tatctcagtt ttgtctatct cgattgctgt ttctaatttt
    11461 tcattatttt tctcaaattc aacaacagtg tatcctagtg ctggcacatt tacatttatt
    11521 tttatgtcaa gttcataata agctggttca tcaaaatatt cgaaacctgt agcaatcatc
    11581 cgataagcat ttttccgttc tggaaaataa acttcttttt caatgatatt atagggacta
    11641 cctttgaagt gtattttctt tgaatcagct actacatgca cgattttcga tccactataa
    11701 ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt ttagctttaa tgcatcagcc
    11761 atccgtttta caataaggtt ttcgattccg tcagctatct cattagcttc tttgattcga
    11821 tggaaaatat cttccgcaac attatctgaa acacagccac caatactatc atgagcctgg
    11881 ttttgcaaaa tctttttcca taaccgacga agtaggcctt tactgatatg gataccattt
    11941 ctttctgcaa tcagcatgag cggctcaact cgttttagaa ttttttgttc caatttaaaa
    12001 ttttctattt tcatttttgt tcttactgag ccaatagaac gatgaacacg agagtaaacc
    12061 ggctgacgaa gttcaccttt atattcaggt aattcttttt tgtttctgat aacttctaca
    12121 taagaaggat aatctgaaat aatattgtgg aatttactgt atttattcaa cttctctacg
    12181 acttgatcaa aatttaaaat cattgacttt tgatctacac ccgaaggtac taaaatatct
    12241 ttgttttttc cttgcatatg aagcatttca actgcttcat ccaacttttc ttttgcaaat
    12301 tcaggaatat ctttaattgc gtctaaggtc atcattccag tcatataacc aaatggaaaa
    12361 ttcatggcat aaacaaattt atctcccaat cctttccaga taaaataagg agatggaacc
    12421 atcgtttcaa agtttaatcc tcgccaacac atgaaattat cgatttctac ttgatgcaaa
    12481 agtgtaggca aattcgcatt gaacccaaac gtgtcaggca aataccctac catcattgga
    12541 tctccatatt taaggatcgt atcactgatt ccaatcatta agtttcttaa aattgattca
    12601 gcatccacta ataaggcatc tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt
    12661 ttcatcagtc gttggataac cttaagcttt tgcggattga tctccaaata ctcatcgacg
    12721 attgaagttt gtccatctaa acaaaagttt actttagggt tcctttccaa ttcatctaga
    12781 acttcagtaa agacctgatc agacaatacg atggcatctt catttgtaaa ataccattct
    12841 ctgtcccagt gcgtgtgttg aattatatgt gcatttacca tatcagattc tcctactttc
    12901 cttcttctac tgaatttact tgttgttctt ttttatactt gatggatttt agtgcattga
    12961 ccattaacgc tgtaaccact cccccaataa caattgatac taaatataaa ggtatattcg
    13021 taacaaaagg aattgccata aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc
    13081 catttagacc acatgctact gcagtgccca ccacgatgct tggtattact cgtaaaggat
    13141 ctttcacggc aaacgggatc gtcccttcag taatccccac aaggcccata atcatagctg
    13201 tctttgcatt atctcgttcc gattcatcat aaagtttttt gtttaaaatt gttgctaaaa
    13261 acattccaaa tggcggaata gcaattccta ataacatggc tgtactaggt ccaaagcttc
    13321 cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt attgataggt cctcccatat
    13381 caaacgccat cattcctcct aaaatagccg acatcaaagc agcatttgct cctgacatgc
    13441 cttctagcca attagtcagt cctgttgtca ttgcttgaat aggcgtagct agtacatacc
    13501 ataatatgat tccaacaact aatgtagtaa caaaaggaat cagcataatt gattttagac
    13561 taataacttg catcggtact tttacttttt tcaatgcatt agctaaatag ccacaagcta
    13621 atccagcaac cattcctcca ataaatccca taccaagatt ttgacacatc atcccagtga
    13681 taaaagctgg tgctagaccg ggacggtcag caattgaata tgcgatatag gctgaaatca
    13741 tcggtacgat aaactgtaat cccgtctgac caatcattcg caggactccc cagaatgtat
    13801 ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc aatagaagtt agtaaccctg
    13861 ctgctacgac aagcgggatc atataggaaa ccccactttg gaaatgattt agaatttctc
    13921 ttccttgttt cttgaattcc attagtaact cccccacgat atatttttta tttgttaatt
    13981 aatgcaattg cttcatcaat cgtctcttca ccatctttta ctacctctga ggtccctact
    14041 gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa catttgtatc tactgcaaat
    14101 atgacaacat ctgcctcgga aacatccgaa attgaaagtt tgttttctat ccccatagcc
    14161 ccctgtgttt ctactttaat atctaaccct ttcttcttcg caaacttttc taaattagct
    14221 tttgccatat aagtatgggc aatccctgcg atacatgctg ttactgctac tatttttgtc
    14281 atatgaaatt cctcctttta aaatgataga gcttttacca attctttttt atttctcgaa
    14341 tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc cagataattc tgctaaaata
    14401 cgtaaatgat tagtaccttt atcagtgtct tttattgcta acaaaaagac gagatcaact
    14461 ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta acttggctac agccactgtt
    14521 gattcaatta ctgctaggct tttgccatga ggaatggcga tatttttccc aattcctgtg
    14581 gtcatatggt tttctctgtc aaatacgtct ttttgaaatt gtttgtggtc agacacaaac
    14641 ccattagtct ctaacaattt cgataatttt tcgactacac tttttttatc agatgcattt
    14701 aaatctatca ctatattatt tatattgatt ttttcactga tcatttataa gccttctttc
    14761 aatatataaa ctaatttttc aaacgacgaa ctcgttttta aatttgctaa taactcatga
    14821 ttcgaagcga tttcattgaa tgtttcataa attttattca attcaggttt tactttttca
    14881 ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat cccaatcaat tccttctgga
    14941 ttaacgtaga cacagattac tggctttttt ataaaatcta cttcgatatg cggcatagca
    15001 aaatcattca tcgctgttga agatagtttt tctcttttga ttgctccctc tagtatccct
    15061 tctttcgcgt aattttcttt taccaatttt ttggtaatta tatttaaaac gttcacttga
    15121 ttatccttta tttggtgggt aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca
    15181 tcttctgttt gttcatttaa ctgtagatac tgttttactt ttctgacatc tgcccctaac
    15241 atcaaaggac taacaacaat aacaggaaaa tttgtatttt caatcggaat cgtgcttata
    15301 acaagttcat cagtgacttt tctatcttgt agttcattga tgcttagtac atcttctatc
    15361 tcaaccatat ctgcaaaacc gttatttatt ctttgctcta ataattttga tgttccatat
    15421 ccgctactac aaactaatat cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat
    15481 tgaatatgga gtgttacgta agcgatttca tcttcattaa aatgtataca gaatctctct
    15541 tcgatttcct ctaataaatc tacacttatc ataaatgacc ttttaaaag
//
LOCUS       DASJOX010000059        15532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJOX010000059 DASJOX010000000
VERSION     DASJOX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.060"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..966)
                     /locus_tag="U4A12_001718"
     CDS             complement(<1..966)
                     /locus_tag="U4A12_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774074.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEF"
     gene            complement(1178..2074)
                     /locus_tag="U4A12_001719"
     CDS             complement(1178..2074)
                     /locus_tag="U4A12_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1774075.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2067..2747)
                     /locus_tag="U4A12_001720"
     CDS             complement(2067..2747)
                     /locus_tag="U4A12_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1774076.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2748..3530)
                     /locus_tag="U4A12_001721"
     CDS             complement(2748..3530)
                     /locus_tag="U4A12_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774077.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3523..4440)
                     /locus_tag="U4A12_001722"
     CDS             complement(3523..4440)
                     /locus_tag="U4A12_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1774078.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4604..4927)
                     /locus_tag="U4A12_001723"
     CDS             complement(4604..4927)
                     /locus_tag="U4A12_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774079.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            5715..8942
                     /gene="fss3"
                     /locus_tag="U4A12_001724"
     CDS             5715..8942
                     /gene="fss3"
                     /locus_tag="U4A12_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1774080.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9055..9369
                     /locus_tag="U4A12_001725"
     CDS             9055..9369
                     /locus_tag="U4A12_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1774081.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            9382..9756
                     /locus_tag="U4A12_001726"
     CDS             9382..9756
                     /locus_tag="U4A12_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1774082.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            9766..10101
                     /locus_tag="U4A12_001727"
     CDS             9766..10101
                     /locus_tag="U4A12_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN1774083.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            10184..11524
                     /locus_tag="U4A12_001728"
     CDS             10184..11524
                     /locus_tag="U4A12_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1774084.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            11602..12276
                     /locus_tag="U4A12_001729"
     CDS             11602..12276
                     /locus_tag="U4A12_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1774085.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            12269..12433
                     /locus_tag="U4A12_001730"
     CDS             12269..12433
                     /locus_tag="U4A12_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774086.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            12509..13651
                     /gene="dcm"
                     /locus_tag="U4A12_001731"
                     /pseudo
     CDS             12509..13651
                     /gene="dcm"
                     /locus_tag="U4A12_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            13641..13931
                     /locus_tag="U4A12_001732"
     CDS             13641..13931
                     /locus_tag="U4A12_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774087.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            14190..15374
                     /locus_tag="U4A12_001733"
     CDS             14190..15374
                     /locus_tag="U4A12_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1774088.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            15371..15508
                     /locus_tag="U4A12_001734"
     CDS             15371..15508
                     /locus_tag="U4A12_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1774089.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4943 a         2827 c         3174 g         4588 t
ORIGIN      
        1 aaattcattt aatttaattc ttactaactc ttgtagctca acacccatat ctaaatagga
       61 aagcttcgtt tgatctaagt cgtgattcat agcatttttt tcaatcgaag taatgtgaat
      121 gtcaacataa gcttgttcat ctattggcac aataattgaa aatgcttttt caatcatttt
      181 gattaaaata agtgtattct ttgagatttc ttcttcagaa ttattactac gtgataagaa
      241 attgcttgtt tcaatccttt ctatcgcaat agcaaggtgg atagctaaag tttgaaattc
      301 ataatctgta aatttcaatt gtgtcttatt catgtatcga ttaagcaatt cggctacttt
      361 tttcaatatg tccttattta agaaattatt cgcttcacca atgaaattga tattcaagtc
      421 taagagatct gttttactgt tagatgctgt aaagccaccc caataatagt tcacaacttc
      481 tgacatcaac cttcttttac ttttttcatc agctttgatg cgcacgccat ttcgatctga
      541 aactaaggac atcccatgct tgtttagtct ttttctaaca tctttcattg tgctttctac
      601 cgtcgacctg ctcacgtata attcgtcagc aagattttga atctttagag aatcattagt
      661 attcaataat ttagcaaaca cataagttag acggtcctcc ggagagtctg cgatattgat
      721 tttatgactg gataataaat ttcgaagtgt ttcttttttt ccaataagcg aaacgccttt
      781 tttaggttga accaacattt gtacatctgg cattttttgg attatttctt tcaaaattaa
      841 aatatctttg ctaattgttt tcgtagatac atataattgt tctgataatt ctttgtaagt
      901 tgtgtgctca ttttcaatca aaaagtatag tattttcatt tgccgttcgc taaattcgat
      961 gcccattatc ttcacctcct gaatacagct taagtgtaca attattatct ctattttcat
     1021 acagaagaaa gttcttgatt aatgtagaag tttctaatat tcttgattat ttcaaatcaa
     1081 taaaaaagcc tgcaatattt atatcacagg cttttttctc tattaaatat atataagata
     1141 atacattttt ctactagttc tcactaatct tgtataatta aaccctccgc aacttgacgg
     1201 agatggagaa gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca
     1261 atttttttga caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt
     1321 cggtataaaa acgctggaag actttttcaa cagaggtttg cgttcctggt ttgataggat
     1381 tggccactgt taatcgaatg aatggatcct cttttgaaaa tgtcaccttt gcttgtccat
     1441 ctgcataacg caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat
     1501 ctgctaatac agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag
     1561 aaaattgttc ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat
     1621 ggagatccat ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca
     1681 tactgagtaa tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg
     1741 tatcctctac ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga
     1801 gatcatgaga aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt
     1861 gtcctttttg ttcattttca tccaaccatg tattcagtgc atctactagg tccattagat
     1921 tcttttctcg aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg
     1981 cgattttctt agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa
     2041 agataacaac gagaagcaac agccatttaa tcaaatctaa accccactcc ccacaccgta
     2101 tgaatataca cagcatgtgc ctttgctagc ttccctcgaa gattggaaat atgcatattc
     2161 actgttttct cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc
     2221 gaaaatactt ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc
     2281 aaatggacag ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc
     2341 ttgtaataga tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg
     2401 atgccgattc gtaacaaaag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct
     2461 cctgctgcca acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc
     2521 ggaacattcg atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc
     2581 atgagatcta gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta
     2641 cctgaataag catcaagaag tttgtatctc tgttcaagaa cctcctccag taaggaatga
     2701 acagcttcat cgtcttcgat aatcaaaata gtttccagca ggaccaccta cttaatgtca
     2761 gatttttgga aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc
     2821 agaatcattt tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc
     2881 cacgtttccg taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg
     2941 ggaatgaagg ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt
     3001 ctagtgatga atgtcaaagc cactggaaca gataatgtcc ctaatgtcag cagaagcaat
     3061 acaggaaata cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat
     3121 ccgaaagcaa gtgtgtaagc aagcatggct agtcccgtga tcaaaaccac gccaagtgtt
     3181 gtgatcaaaa gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa
     3241 gataacgtat tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat
     3301 gctgcgaaag ccagaagaat ccatgcagcc attttttcta ccattaaaat ccctgttgca
     3361 ccgttgatga ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac
     3421 cattcgttcc aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt
     3481 ttacggctct tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc
     3541 tccagttaat tgtaagaaat aatcttccag tttttgccca actaggtgaa taccatccac
     3601 aggaactttt gcttctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa
     3661 aagctggata acacggctag attgtacaaa ataatcccta taaccaagag agtcgagaac
     3721 cgttactgct tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc
     3781 caattcttct ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac catagtgagt
     3841 ggcgatctgt gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc
     3901 ttttaccaaa cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt
     3961 cgtcggttca tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa
     4021 acgctggcgc atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg
     4081 catcattttc agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat
     4141 acgtaggtta tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc
     4201 catacgtcgt cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc
     4261 aatcgagatc aaacccataa tagttctcat aaaggtagac ttacccgctc cattttcccc
     4321 aatcaatcca tagatcattc cacgtttgat ttcgattgat actttatcta atgctttggc
     4381 agcaccgtat tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat
     4441 acttatcctt ctttcttatt gatgagtgaa gtatagttga cgggtataaa gaaagcagcc
     4501 agaaatgtat aaagaaagtg taaattttcc aattgaatgt caaaaaaaac aatcggacaa
     4561 tacgtgtctt tcctattatc aggcagtttg ttatacgctt gtatcagttt cctgattctt
     4621 tcttttcgac caaagtcgtt tctctgtatt cctcttcact gaaaattttt gcttctttca
     4681 gatcaagctt gagtaatttt accagaccgg ttataaggat aactatagca agactccctc
     4741 ccagaaaaat tagactgtct cgcagacgtg gttgcgttgt cttccaatca aagagcgcac
     4801 caaacagtgc taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacataa
     4861 gtcgtccttg tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag
     4921 aaaacattaa tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca
     4981 attagctacc aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa
     5041 acggtgttct cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc
     5101 aatttttgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag
     5161 cggataaata atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat
     5221 gatattatag accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt
     5281 ccaaattgtg gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc
     5341 gcccagtacg attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga
     5401 actgtgaagc tttacgggct ttcttaagac ctggtttttt cttcgtatca ttgaataagt
     5461 ttgaaaaacc ttgatttata aaggttttcc cgtaaatata ttttattata tttgattaaa
     5521 tttgaaatgt ttgtgttcta aaatgtgttc taatttaaag gcagtatatt tcgtatttta
     5581 gattaaattt ttaggctcat tcattctcaa atccattcta ttcagtcaat aaaaatttta
     5641 aaaagacttt ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga
     5701 aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga
     5761 gtttggctgt tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa
     5821 ttacccatcc acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag
     5881 gaacttttag tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca
     5941 acggctttaa acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca
     6001 ctcctggcta tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg
     6061 ttttatggaa aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct
     6121 ggcaagaagt taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa
     6181 atatcgcagc cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa
     6241 ccttccataa tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga
     6301 ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc
     6361 gtgtgaatgg taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc
     6421 tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac
     6481 agactttaat ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg
     6541 aaacggaggg ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag
     6601 gaacggtttt ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca
     6661 aagaaggcat tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa
     6721 aatcagtacc ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag
     6781 gcgaaaccat ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata
     6841 aaagcggagt tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata
     6901 cgtttgccat tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg
     6961 aaaacggtca tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt
     7021 atgtgaccga aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg
     7081 aattgaagta tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc
     7141 aagaagtcac tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac
     7201 aaggtaaggc agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag
     7261 ctgttaaatg gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg
     7321 aaacggtcac tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca
     7381 acgagtatta ttggcaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt
     7441 atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg
     7501 ttacggcaaa agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag
     7561 ctgcgggcac taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag
     7621 ggaccaatga aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag
     7681 ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag
     7741 tagaagcccc agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg
     7801 aaaacaaaga agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa
     7861 aacaaccaat taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg
     7921 ttagtttgaa ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc
     7981 ttgcgacttg gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg
     8041 ataaattgag ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca
     8101 ttgatgtgga tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga
     8161 ttgcagaaac aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc
     8221 ataaattaac cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg
     8281 aaaataagga agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata
     8341 accaagccca aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc
     8401 acctagaaaa cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg
     8461 tgtcgattac tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg
     8521 ctttattacc ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag
     8581 taaatgataa agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc
     8641 cagaaggaac caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg
     8701 gaaaacacaa tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa
     8761 ccatactaag tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat
     8821 ctagtcccac agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt
     8881 tcattggttt tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact
     8941 aaggtgatga gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa
     9001 aagtagtcgc gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa
     9061 ttgaaatttg tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa
     9121 ggagaaatta taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat
     9181 ttgtattcca gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt
     9241 gaaaaggatt ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa
     9301 ggctatgaga ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg
     9361 aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta
     9421 acatttggga agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat
     9481 ggaacaattg gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg
     9541 cgaatgattc aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca
     9601 gaggtagagt tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga
     9661 actaccgtta attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa
     9721 ggaaatccta cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt
     9781 gaacatattt tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc
     9841 gttaaagcat taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga
     9901 gaaaatagcg acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct
     9961 ttctttatag ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa
    10021 gaaattggtg tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg
    10081 aaacaactcc cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg
    10141 aaatcctatt gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg
    10201 catacgcgca ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct
    10261 gttcatcggt tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa
    10321 gctatcactt tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg
    10381 aagtctcgct ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg
    10441 gcgaagcttg tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga
    10501 agtgaaacaa actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga
    10561 gactatcagt tacttcccca aaatctgtta tcgaatgaaa gatggattac tgtctattcg
    10621 cgttcaaatt tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga
    10681 aagcggactg tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac
    10741 tttactgtat gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg
    10801 gactctgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat
    10861 cgctggtggc acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt
    10921 gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg
    10981 tacggtgatg cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt
    11041 ctatgaacgt atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg
    11101 agagaattat gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc
    11161 ttatatggag atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat
    11221 cgtgatgttg ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc
    11281 gaaatattta ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat
    11341 gagtgaactc ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca
    11401 catcaaaggt cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc
    11461 acttgtacca aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga
    11521 ttgagtgatc ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta
    11581 ggaagaaagg ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca
    11641 aataaggata agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac
    11701 gcagttttta tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct
    11761 gagaattatg ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat
    11821 tataatggaa aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca
    11881 tttagatatg tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta
    11941 agtgctgaaa aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat
    12001 gtttatcaag accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga
    12061 cgtcatgtgc cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca
    12121 aaagtaaaga gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat
    12181 aatatttccg aagttcatta tgctacggat aatctagaat tatttttacc actagggtat
    12241 agtaggaaag atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt
    12301 aaagaaatac aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa
    12361 aacggtgatt aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt
    12421 aaataaaaaa tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct
    12481 tttttctttg caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg
    12541 tggctttcgt ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga
    12601 taaatttgct agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca
    12661 tgacatcaca agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg
    12721 cggcggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac
    12781 tcgaggtact ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt
    12841 attccttgag aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat
    12901 cctccgaacg ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc
    12961 ctacattccc caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac
    13021 cgaacaagta tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt
    13081 ggtaaatatc aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt
    13141 agcgccaacc ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat
    13201 ctctgtagca ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac
    13261 tggaaccgcc cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa
    13321 tgaaacagaa tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc
    13381 cacaagtcgc cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca
    13441 acaagcgatc gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg
    13501 acttcagggc tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca
    13561 gttgtataag caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag
    13621 attaaaagag ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga
    13681 ttggaaagat tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata
    13741 ttcgcaagaa tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg
    13801 ctcgaattga aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag
    13861 aaccagatat tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta
    13921 cagatgagta acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg
    13981 aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt
    14041 ctcacccccg tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa
    14101 gaatgcttgt tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa
    14161 gttaacacct tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat
    14221 taaaagaccg acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac
    14281 ttagtcggcc gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca
    14341 tgaggaaaat ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt
    14401 ttgactatgt aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc
    14461 tccatttgaa atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac
    14521 agtatgtcat gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt
    14581 tagaattaaa ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta
    14641 gctggtacga ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact
    14701 tggcaatcaa tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga
    14761 aggaagaatg tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa
    14821 aggctgatga aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg
    14881 tttatttctg tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag
    14941 accaaactga gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg
    15001 cgattcaaga tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc
    15061 gttacttgcg atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg
    15121 aacaatgggg gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa
    15181 aaccgtatac cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga
    15241 aaatggctaa ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga
    15301 atgcacgctt atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa
    15361 atttgattat atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt
    15421 tggtggcagt atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga
    15481 tcgagcaatg gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DASJOX010000060        15047 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJOX010000060 DASJOX010000000
VERSION     DASJOX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.061"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..473
                     /locus_tag="U4A12_001735"
     CDS             <1..473
                     /locus_tag="U4A12_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1774090.1"
                     /translation="HPSNITVEVTGLAASAATFIVAGANDVIMNVGTSLMIHEASTFA
                     WGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLREWMNEEKWFTADEAVKYGFAN
                     SVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIEQESKQKSLIARLRKGE"
     gene            478..1638
                     /locus_tag="U4A12_001736"
     CDS             478..1638
                     /locus_tag="U4A12_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1774091.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            1714..1992
                     /locus_tag="U4A12_001737"
     CDS             1714..1992
                     /locus_tag="U4A12_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1774092.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            1973..2344
                     /locus_tag="U4A12_001738"
     CDS             1973..2344
                     /locus_tag="U4A12_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEN1774093.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            2360..2764
                     /locus_tag="U4A12_001739"
     CDS             2360..2764
                     /locus_tag="U4A12_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774094.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            2761..3171
                     /locus_tag="U4A12_001740"
     CDS             2761..3171
                     /locus_tag="U4A12_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774095.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            3232..3819
                     /locus_tag="U4A12_001741"
     CDS             3232..3819
                     /locus_tag="U4A12_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1774096.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            3892..4359
                     /locus_tag="U4A12_001742"
     CDS             3892..4359
                     /locus_tag="U4A12_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEN1774097.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            4405..4713
                     /locus_tag="U4A12_001743"
     CDS             4405..4713
                     /locus_tag="U4A12_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774098.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            4908..11642
                     /locus_tag="U4A12_001744"
     CDS             4908..11642
                     /locus_tag="U4A12_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1774099.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            11646..12518
                     /locus_tag="U4A12_001745"
     CDS             11646..12518
                     /locus_tag="U4A12_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN1774100.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            12515..13564
                     /locus_tag="U4A12_001746"
     CDS             12515..13564
                     /locus_tag="U4A12_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774101.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            13568..14392
                     /locus_tag="U4A12_001747"
     CDS             13568..14392
                     /locus_tag="U4A12_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774102.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            14410..>15047
                     /locus_tag="U4A12_001748"
     CDS             14410..>15047
                     /locus_tag="U4A12_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN1774103.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         5387 a         2346 c         3214 g         4100 t
ORIGIN      
        1 atcacccatc aaatattact gtagaagtta cagggcttgc cgcaagcgca gcaacgttta
       61 ttgtcgctgg tgccaatgat gtgattatga atgttgggac ttctttgatg attcatgaag
      121 cttctacatt tgcttggggg aataaacagg acatccaaaa aacgttaaac gcattggaaa
      181 cgatcgatga ttctatctta gctatttatt cagataagac tggccaatct gttgataagt
      241 tgcgtgaatg gatgaatgaa gaaaaatggt ttacagcaga tgaagctgtc aaatatggat
      301 ttgccaattc tgtgaagcgt gaagaaccac aaaatgaacc tcaaaatatt gcagcgatga
      361 ttcaagatgc tgttgccgtt gcaatagcga atttaaagga acctgttact aataaaatcg
      421 aacaagaatc aaaacaaaaa tcattaatcg cacgattgcg aaaaggagaa taaagatatg
      481 ttaaaaatta cagataaaac tgcagatgca aaaaaggcat ttaatgctat ttcttcgaaa
      541 gaagatgcaa cacccgaaca aattaatgct gctctagaag aatatgtaac cgccattgca
      601 gaagatgccg gcaaacaggt gcgtgctgaa tatgaagaat tgaaaaatgt gacggacaac
      661 cgtgtattag aggctcgtgg aattcctaca ctaacagcag aagaaacaaa gttttataat
      721 gaagccgtaa aatctggagg tttcgattct gacgtagttt ggccagaaac gattttggaa
      781 cgtgtttttg aaaatcttca aaacgatcat cctattttag gcctcattaa ctttacacca
      841 actgtaggtc gtgtcaaagt aattagagca cgtcgtaaag gtgttgctgt ttttggacca
      901 ttgcataaag accttgaagg ccaactagat gctgaatttg gtgcaactga atttgttcaa
      961 cttgcgttga ccgcattttt cttaatttct aatgatacct tagatttagg accacgatgg
     1021 attgaccgtt ttatcaatct ttctttaagt gaagcagtac gtgatgtttg ggctaaaaaa
     1081 attattacag gttctggtaa agatgagcca attggattgc ttaaagatct agatggtgcg
     1141 gtcacaagtg gtgtatatcc agataaagct agcgcaggga cattaacttt tgcgaaagag
     1201 aagattgttc ttgaacttgc aggggtgatg aaaaaattat ctaaatacac atacaaagta
     1261 gatggtgacg atgaaggtga agtgaaatac cgttctgttg ctggacgagt gtatctaatt
     1321 gttaatcctg ttgattactg ggatatcgta gctagagtta catttgcaaa tttaaacaat
     1381 gtgtatggga ctacgatgcc atttatcgct gttgatcata tcatcgaatc tgtagacgta
     1441 ccagaaagta aatgtattgc atacgttgat aaagagtatg aagctactca atcaagagct
     1501 gaaaaagtct atgtatataa agaaactttt gcaatgaaac gcgcaacttt gtatgctgtc
     1561 gacatgcttg gtaatggcta tccaacaaat aatgatgcag cacaagtata tgacttggac
     1621 tttacagcgg gggagtaatg tcccctgcaa ttggtgagat aacaccgacg actgatgggg
     1681 cagtcattaa tctttcgtaa atggggttag agtatgaaca ctgaagaatt tataaaagaa
     1741 tataaggccc gatttcgtat tttccatact tccgaagatg aggatatcgg gaaacagtta
     1801 gagagcgggt ttgccgatat aaaatctcta atcggcagtt ttgatccttc cacttatgag
     1861 aaaggaaaag aattagttta tgaacgtact cggtatctac ggaacgaatc actagagtac
     1921 ttttacgaca attttcaaac aatgattatg gatgcatcaa ttgatctggc aggtgctgga
     1981 tatgccaatt aaatcaaatt ataagaagcc taaaattgta gctggtgatc taaatacgcc
     2041 agttactttt tttgagtttc aacctaataa gggtccagaa cctggtgatg aagaaaaaaa
     2101 ggaactgtat ttttgtacag cactagttta caacccatca atgaaagatc gtgacattct
     2161 tggcacaaaa gaaggcgtta cgataaaaat acgggatccc catcaatctt atattcctac
     2221 taacaaacat aaagtaagga tattagatta tcgatatgga gataaagttt gggaaataat
     2281 cgatgtctca ccggatttgg aagaaaattc aattgtaaag atagtattag aattaattac
     2341 ttaaatttgg tgataaatga tgagtaacgt aaagtttgaa ggcgttcaag aaacaatcca
     2401 agctttagaa aaaaagtatg gagaaaagaa aactaagaca ttaacaaaaa aagcaatcaa
     2461 tgttggcgct gaaaaagtag aaaaacagtt gcaaacggac atgaccgttt ttaaagacaa
     2521 aggttatacg atcgctgagg ttgttcgcaa gaacgctacg tatagagatt acaaggcaga
     2581 agctgaaatc ggttggaatg gtccacatca acgatataga atcattcatt tgaatgaatg
     2641 gggatataca cgaaagggca aacaaattag accgagagga tttggtgtaa taacaaaatc
     2701 tcttaagaat tccgagctgt tgtactttga tgctgtagga gaggaggtta aaaagagttt
     2761 atgaaagata tgcttaatat catttacgag gctctttgtt tgaatgagta tattcattca
     2821 atgacctaca acgagaatac cgagcaatat cgtattaaat tttatgagca acctgaaacc
     2881 gccgataaaa caggtccaat gatcacaatt cgtcctgtag atacacctaa cgaagcgtac
     2941 cacggaagta acaaagagct ttctgtggag catatgatac agattgacgt tgaatcgaaa
     3001 tatagagcaa catgcaagca gattcaatat gagataaaaa aagagatgaa aaaactaggc
     3061 tttggccaaa tttccggtca agggttagat gaatacttta ctgaaacaaa ccgctttgta
     3121 gacgcgcgtc gttacgaagg aaatacaaaa atttacgata ccgaatatta gaaattaaga
     3181 cacgaaaata acgtgtcttt ttttattacc caaaattagg aggaaaattt tatgacttta
     3241 gtaggattca aaaaaatgac aatcggggtc tttgacagca cagggaagat tccagcagca
     3301 aatttatatg taattgaagg tgaacaaaac aaaggggcga ctgtatctgc tgaaatcagt
     3361 gggctatcaa aagaaccatc aaaagtttac ggatcgaaca tcgcttacta tgtctctcaa
     3421 aaaggaacag gcgatgtttc agctactttt ggattgttag atttaccaac agaagtaaat
     3481 gacaaaattc ttggttacaa aaccgataca aacaaaatca gcttcttggg agaagatact
     3541 gagccaccat attgtgcaat tttgatggaa tcggaggatt tgaacggcga tacagcaatg
     3601 cttgcgatgt tcaaaggaaa attcagtcgt gaatctatta atcttaacac tacgacaaat
     3661 gaagctttcg aaccagaagc agaagaatat gtattttcag cgattgccaa tgatgttgaa
     3721 ggtgatgcta aaggccaaac agtcgcaaaa tatgttggag acgaagaagc ttctattact
     3781 ggtttgaaag agatggtatt tcctgcagcg ggggagtaac tagccccgtt gttggagcaa
     3841 tcactcccac tattaatggg gcaacagttg aattaagtta ggaggataac tatgccagat
     3901 acatttaaaa tctataaaaa aaatggtact gaatttacaa aagtcgcaga gggtgaaagt
     3961 ccactttcta ttactggaat tgcagcaaat actcaagttg ctaaaggaga ttatcaagct
     4021 acgcgtgtgg tcggaggtca agaatcagta aaagtagata ttcctgcatt caccactctt
     4081 ccaatttcag ttactggtgt gacgttggat aaaacgacag ctgaagttga acaaggcgga
     4141 accttaaaat taactcctac tgtaacacca gcaaatgcaa cagacaaaac aggaagctgg
     4201 agcagttcga atacagctgt agctacggtt actggaggca acgttacagt aaaaacagac
     4261 gctgaagttg gcggaactac agaagtttca tttactacta cggatggcgg taaagtagca
     4321 aaatgtacta ttacagttat tgaaaaagca gcgggctagt cttcggacta gcttttttat
     4381 ttggaaaata atggaggaat ctaaatggca aaagtaagaa ttgaattgaa agaagaatca
     4441 ggaaagaaag tctatgaaaa catagacaca acagggaaag attatcgaaa agctttagaa
     4501 acaatcaaaa agctaaatga agacggagta atgatttgga atcgtttaga catttatttg
     4561 gattttgctg taggtatctt cagggatagc aaattgacag ctgaacaaat tcttgatggc
     4621 ttaccatctg agaaggtaat gagcacattg gacgacatcc taggagaagt aatggggatt
     4681 gaaagtaatc cagacccaga cgcaaaaaag taacacccga agaagcagaa gaaatgtact
     4741 taaacctatg cagagagtta gtgaaagcgg gatggtcact atctgacata gaagataatt
     4801 cctttgaaat gatgatgaaa attgcgtgca cttcgcctaa gaaagagaaa caaaaagaag
     4861 ttgatcttaa agacttcttg aaatccatgt agagaggagg taaacatatg gcaaatggaa
     4921 aacctttagg caatatgatt atcaaactag acttagacag ttcagctttt tcaaaaggtc
     4981 tagccggagc taaaaacgct gttaaccatc aaatgaaagc aatgaaatca cagatgcaag
     5041 taatgaactc ttcaggaaat tctctcggtg ctttacaagc aaaatacaat ggtttaggcg
     5101 gagttctaca agcaaatgaa aagcaagtag aattgctcac taaggcttat aaggatagtt
     5161 ttgactctaa cggaaacgct actgcttcga ctgctaaata cgctaatcaa ttgaatcaag
     5221 caacagctag atcagcaagt tacgaatctc agatgaaaac aactgttgga cagattgctc
     5281 ggatgaaagt ggaaacggaa gggtttacgg ggaaactaaa ggctaattct gatgtattga
     5341 ttaattctgg gaaaaaaatt gaagcctttg gtaaaggagt ttctgggata ggatctgccc
     5401 taactgttgg agtaacagct ccaatagtgg caggcgcaac tgctgttact agtgcagcta
     5461 tttcatggga aagtgctttt gcaggtgtaa aaaagacaaa tgatgaagtt gtagactcga
     5521 atggaaacgt agtttattcc tatagcgact tagagaaagg tctgcgtaat cttgccaccc
     5581 aattacctgg tagccatcaa gaaatagcaa atgtagccga agcagccggg caattaggta
     5641 taaagactga aaacgtagtt agcttcacta aaacgatgat agacatgggt gaatcgacta
     5701 atatgtcggc tgaaactgcg gcaacttctt tagcacgatt tgctaatatt acccaaatgt
     5761 ctcaaaaaga ctttgataaa ttaggatcag tcattgttga tctaggtaac aattttgcta
     5821 caaccgaatc agaaatcact gaaatgggat tgcggttagc gggagctggt aaacagattg
     5881 gaatgagcca aggcgaaatt ctaggatttg ctactgctct atcttctgtt ggtgtcgaag
     5941 ctgaagcggg tgggtctgcc ttttcgaagg tcatgatcca gatgcaacta gctgtagaaa
     6001 aaggatttgg cgcttttgat cagctgaaac agatggcaga agaacaaggt gttccgtggg
     6061 ttaatttagt aaatgctgtg agagacggtg gaaaatcgtt aaaagcagta tcagagcaaa
     6121 tgggattcac ttcatctgat ttaaagaaaa tgtataaaga agcggacaat tcgaaaagtt
     6181 cgcttgaaaa ttttgcgaat gtggcaggtg ttacaagtga acaattccag aagttgttca
     6241 agagtgatcc ttcagaagca atcatcaagt tcattcaagg gctgaaagat tcagaaaaac
     6301 atggtacttc agcaatcaaa gttctggatg acatggatat aaaagaagtt cgactacgcg
     6361 acagtttatt gagagctgca aatgctagtg acatattcaa aggagcaatt gaaaaaggaa
     6421 acggcgcgtg gaaacaaaat acagctttaa caaatgaagc taataaaaga tacgaaacaa
     6481 ctgaatcaaa gctaaagata ttgaaaaacg aagtgattga tacagcaatc gatcttggag
     6541 gtccatttgt agacgcgttg cgtgatggtc taaatgtatc gaaaccattg ataaaaaact
     6601 tagggaatat ggcgaaagct ttctcagcta tgagtgatga tcaacaaaga aacattatca
     6661 aatgggttgg attagcagca gcagctggtc cagcgctgaa gcttttagga actggtactt
     6721 cagtaatagg taaagttact tcaactactg gaagattgac gaaaagcctt gttgatttag
     6781 cagcgaaagc agcagaaaag aaggctatgg atgcctttgc atcgtcggta acaacagctg
     6841 gtgctgcagc tgcaaagaca gcaggagctg gaggtattgg cgggttagtc cctatgatgg
     6901 gaaaaacagc tagtgctgca agtactgctg cgggcgccag cggaataggc gcaatgacaa
     6961 cgtcactagg tttgttaggt cctgctctac ttggtattgt tggcgttggt ggcgcactgg
     7021 cagttggcta tggagcatgg aaattatttg gtgaagaagc ttggaattcc tcgcaaagag
     7081 taaagcaatg gggaacagat gtcggtgagg agatagatag tactcttgac ggagtacaga
     7141 agaatattga aggtgcaaat ggtcaattcg gtcttctcaa agagggtttt actgttgatg
     7201 atgcaaataa tatggcggct aatttcgcaa ccgctggaac atctttggaa acatctttaa
     7261 cgggtagaat aaaggcatta gacgatgcac taaaagggct acctcaatca gttcaggatg
     7321 ccatgaaaga agtagctgaa tcagaagaag aaaccttagg gcaatctctt tctaaaataa
     7381 aagacaacaa tgaagaaata aaaaaaatca gagaaagagc cgcaaatcag aacagagact
     7441 tgactgtttc agaactacaa cagatacagg atctaatgaa aggaacttct caagcgtatg
     7501 tggatacgct cgatgtttct gctaaagaca gaaaaaagat actatcagcc atgaatggtg
     7561 atgttgcaca agctacagaa gaagaagcga agacgtggtt acaatcttta gcgaaacaaa
     7621 aagctgcaac taccgaaaat tatagacaaa tgcttgaaga aaaaaaggcg gctttaaagg
     7681 aaaagggagt aagcaaagaa ttcattgatg cattagaaga tgaaatgaat gattacatca
     7741 aaactacaaa tcaaggattc gatagccaaa tagctgctat aacaaataaa tatccacaat
     7801 tgattaacga agtgagttta tcaaatggtc aattatttag caaaacggca gctgctatgg
     7861 acaatactgg tcagtataca aaagtttttc tcgaaagtaa tgaaaagatt ttagcccaag
     7921 cgaagaaaac atcggatgaa atagcagata atgccgaaaa aaatgcagaa caacttggta
     7981 tggtagctga tgaggctacg catgctggat caacttggaa tggcttagtt ttagacgaaa
     8041 aaacaggaaa agtaaaaact aatgtacaag aagtagtgaa tgaagcaacg aaggatttca
     8101 ctaaatggaa tgaattgaaa cctgttgttc atgatgcgaa actaacaacg aatgcaaaag
     8161 atgtcatttc catagcggca gtacaaaatg gctattggga tgatatggaa tggaaagaaa
     8221 aagaagcatt attagaagat gagtgttcta aaaatgtata caaggcttta gaaaatgctg
     8281 gtaaatggaa tgagttagat attccttcta aagaagcaat cctaacttca aatactcctg
     8341 aagttatggg tgaaactctc atgaatttgg gattatggga tacttacaaa cttcaagtga
     8401 aagatttaga tttgaatgcg tataaagtat acgatgctat cgatggatca gaagaaaaat
     8461 taaatagttg ggctcaatta gataacccca caaaagaatt gttattagat aactcagatt
     8521 tttctacaaa actttttaat tccgaagaaa tgtttaaacg ttggaatcag ttatcatcta
     8581 atgcaaagtt tctttcggct aataatacta aatttacaga ggttctttta tcatctgaca
     8641 gaatgtggaa tcaatgggta aaccttcctg ataatgagaa aagattactt gcagataacg
     8701 aagatttagc taccactgtt ttatcttcag aagaaatata cggaagatgg actcaattac
     8761 cagataatca aaaatatatg ttagctgaca atactgactt agcaaacaaa atattcgctt
     8821 cagaagaatc atggaatgca tggaaagcaa ttccagacac agttaaaaac ttgcttggta
     8881 ataatattga tttactggct aaagtgaaag acgggactgt aagtgtagaa gaatataaca
     8941 aagttatgcc acttcttaaa actctatatg gagataacgt taatttaaaa gaggcaatca
     9001 gtggggctaa aagctctata gagaattaca acaaaaatat ttttcctagt gataaaactg
     9061 cagtaggaca cgatgaggct tcacaagctg ctaaagatgc atttgaagcg ttcaacatct
     9121 tccaaacaaa aataccagat aaaattacta aaactgtatc agctgacttt ataggtccta
     9181 tgccgaaaaa tgcgaaagga acaaacttcc atcctggcgg agcagctatg gtaaacgatc
     9241 aaaaagggcc tacttatgaa gagcttataa ccttgccaag cggagaaact tttataccta
     9301 aaggacgtaa tgtcgtttta gacttaccaa gaggttcgaa agtactgaac gccactaaaa
     9361 ctaaacgtct agtgcctaaa tatgctgatg gtataggaaa cataacgacg gtttcatcta
     9421 cgctaaactt agatgcttta attttagcta ttaacgagct aacgacggta ttgagaactc
     9481 aactgatggc aggaacgact gatgctaaag atactaaact atcagatgaa acaaaagttg
     9541 ccgatccgat tattccggac agtctttctg aaaaatcaga tcagtatcta ggaattggag
     9601 ctcaatggtt aactaatcta atgaatggtt ggaactcagc ggttcctcaa tatatgaata
     9661 gtgaaatggt ttttattaca aactatctca atgcgctaaa acttcaaaac aatcctaatt
     9721 atacgcaagg agcaacatgg aataagaact tactgaatgg atggaatagt ttaactggga
     9781 cctttattgc tacaatcaat tcattttgta atcaagcaat ggtcacgctt agaaactaca
     9841 acactcctat gtacaacaat ggccgaacgt ggcagcaaaa taatcttaat ggttggaatt
     9901 cattgtatgg atcattcata gcccgtgtaa atcagcttgg taacgattcg attaacaacc
     9961 ttcgttctaa aagtggtggt ttttacaatg cagggacatt cttgttgcaa tctttaataa
    10021 acggaatgaa ttcgttaggt aattctcttt ctactacaat gaacaatgtc gctaatacaa
    10081 tggttggtgg tataggcaaa ggagttaatg gcgtcatatc gggagttaac tatgtgctta
    10141 aggaagtcga atcaagtaag aatatcggaa actggccaat tccacaatac gctaagggaa
    10201 ctgatggaca tcctggtgga cttgctatga ttaatgatca aaaaggccta gttcacgaag
    10261 aatatgttca gatgccagat ggacgtagtt ttattgccaa aggtagagat cttcttgtta
    10321 atttgcctaa aggtgctcag gtcttaaatg cttctttaac aaagaaatta aaggaacgtt
    10381 taaatattcc acactacgaa aaaggtattg gcaatttaga tattgttgat ttgttagatg
    10441 atgaaaaaag aatgttagaa ttcttaacta gcaaagttga tttttcaggc attaatgaac
    10501 catggcttga tatgactaaa tcaggaacgt cattaatgtc caaagctgca aatacaatgc
    10561 ttcaatcaaa attaagtgaa ttcttcactc atggcaattt tgatggtgca gtaaatgcca
    10621 atggtgttta tcaatattta gttgatgtgg cacagaaagt aatgggtaag ttccccggac
    10681 ttacagtaac ttctggatat cgagcaggag atgcctacta tcacggaaaa cggcaagcta
    10741 tcgacttggc ttatccaggt atttctggag atccgagata tacagcagca gcaaattacg
    10801 cttttgaaaa attcccttca aaaattgcgt atgtcattac gaatggacgt gtacgtgacc
    10861 gcatgggatt atctggaaca aggtcaagcg gacaatggac gaattggcca gatggcgatc
    10921 acttcgatca tattcattta aacggttcga tggggtctgg cgatatattc aatggaggag
    10981 ttggaggaag tggtgttgca cgctggcgtt cttatgtgtc taaagcattg aaaatgaatg
    11041 gattaccagc tacagcagcg tatataaatg cctggatgtc ccaaatccaa acggaatctg
    11101 gtggtaatga gaaagcaatc ggcggcaatg atgggctcgc cgaaggcaat gcaacaggat
    11161 tacttcaaac gaaacccgga acttttgcag ctaacgcttt tcctggtcac ggaaatatta
    11221 tgaacggatt tgataacatg ttagcagcaa tcaattatgc taagaaacgt tacggagttg
    11281 caggtatgtt gcaagttatc gggaaaggac atggatatgc caatggcggc ttgattacca
    11341 aagacggact atatcgagca ggagaaggaa ataaaccaga aatggttatt ccattgacaa
    11401 gaaaaacaag agctatcgaa cttatgggtc aagcattagc cttcctttct ggaagcaata
    11461 aaaaagaaac gaaaagtgag attaattctg tagataatag acatatccta caagcgatag
    11521 aaaaacagtc tcgtatgaca aatgatctgc tatcattatt actctcgttt tttaaaagta
    11581 acaatcgatc agataaagaa ctagctttag atattcagaa aattttaatc aggaggacat
    11641 agtatttgag gacagtaata ttaagaaata aattgaatga agaaattgat ttgtccacag
    11701 aagattattt tgctactgaa atgtcgaatc taggttttga agttacaaaa gagcatattg
    11761 gacaatgggg gagtttcaga gaaataggag agagtattga attgtctgaa tttcaatcat
    11821 cagttatgat ttctgttcac ggatttagag aacaacaatt atatggttca ttagttgaat
    11881 ttctggctaa tggacctttt gaactagaat tttcttttga ttcagaaaca atgatcagga
    11941 aatgtagttt gaaatcatta tctaaaacag aaattgattc ccaaagctca ttgctaacag
    12001 atactttgaa tctatatttt acttcaagct ggtattctat taagcgtgaa aaattaatac
    12061 agaattcaac ttctctcaat acacgtggaa aagtttactc atttgttcga ccatatgtct
    12121 acaccaaaaa tgtatgggaa aagaaaggtg tatttaagtt cagtaataat tctgtatatt
    12181 tgacaaatag tacagagcga atgtctccgt taaaaataag gattattgga gagtgctcaa
    12241 atccatattg ggaagttatc caaaactcta aagtagtagc ttcagatggc tattttatta
    12301 atatgaaaga cacacaaata ttagaagtgt ctagtttgct agaagaccaa acggcaattt
    12361 taaaagatgt aggtggagtt gaatcttctg tttatcaaca acaagatttt actaaatcaa
    12421 acttcgtcca agcgcctcta ggagaattca gcgttgtttt tcatgttgga catgcaaatg
    12481 tagagattga actttatgag gagcgtgatc tattttgatt ttagccatta ccctctttca
    12541 ccgagatatg aatttatata acgaacgttt aatttcgact gagttcgaat ttggaacgga
    12601 tgaaattgga gaagaagtaa gtaattttat tacagataaa tatgtccatg taaaatcagg
    12661 agattttcta ctagcaaaat ttattcctag cgggaagatt gcttattttg gagtgattag
    12721 ctcgcaagaa gacaaaaaaa taaattgcaa aggattactc agtttggccg atagcgagat
    12781 accgactgta aaaatatcag gtgctaatta tgaagagcat attcgacgac tgattgaata
    12841 ctacttattg aacgatccaa caaaacaact aaaaaatatt ttggaggtaa aaacagaaag
    12901 cgttacttct cattcttatc aagctgttga cacaaataag agacgattga atgcatatat
    12961 aataaatggg ttcaaaaaat ataatgttaa atggtatgtc aaagagatta aaggtaaaaa
    13021 aatatatact ggtattcgag cagttgatga ttcaatttat attaaagata attcatctga
    13081 gtttaatgac tgggatgttt ttgtgcaatc tcctggacca gggaacgaga acaaattatt
    13141 gatcgtagat aaagcaatga aagatataga aaatcccttg gtattgtcta cgtggtattt
    13201 agatgacgaa aataatctta cgcaagattc aacgaataag aacattgcat tgcctaccgt
    13261 taatttagta aatatatatg atcaaactca agaagataga ccttcttatg aagaggtagc
    13321 taaatcggag cttaaaggca acgcctattc acatgaaatt aaagttaatg ttgtgctaaa
    13381 tgctagaaat atcaatgtag aaaatatcga aacaggaatg ttagcgacta ttgtttacaa
    13441 agagcagact tacaaatctg ttctaactgc ctggcgaata tccagcagta aaaagaatat
    13501 agaattgaca tttggtaatg tccgaagtcg ttttatggat tattttgaag ataatggggg
    13561 ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag taagcgcaga
    13621 aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta ttgatggtta
    13681 cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg caggagcagc
    13741 gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag ttccagcaaa
    13801 ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac ctggatctat
    13861 tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag aatttataac
    13921 aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac cactatgctc
    13981 agttaattct actggatcaa ctgtttcaat ctcaaagata tcggatagtt acgagctgac
    14041 tctctctaaa ggagaaattt tgtggagggg gactgcgtta atgcatgata ctcaaactgt
    14101 ccaaccttca aaaaaaattt ggcagacagt ttcaggtttt ttgttagtat ggctcccata
    14161 cgaaaacggg caagcaattg aggatagata tgtaactacg cctttttata aggagcgtat
    14221 aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc acaaaaagtg
    14281 gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg cagcaagcaa
    14341 tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt agttaggagg
    14401 tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca gccttatcta
    14461 cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa tgtacaactt
    14521 cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt gaaatttgtg
    14581 ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga ttcgactaat
    14641 gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca attcaaacaa
    14701 gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa cttcatcata
    14761 gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat ttcttcattg
    14821 gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga taaacaggtc
    14881 caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa tgaatcagat
    14941 aagcaagtaa atgatcgtat tgataaggca aataatcgta ttgatcaaac caatcagcaa
    15001 atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcattga
//
LOCUS       DASJOX010000061        14815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJOX010000061 DASJOX010000000
VERSION     DASJOX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.062"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(296..1024)
                     /locus_tag="U4A12_001749"
     CDS             complement(296..1024)
                     /locus_tag="U4A12_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEN1774104.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="U4A12_001750"
     CDS             complement(1002..2576)
                     /locus_tag="U4A12_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1774105.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="U4A12_001751"
     CDS             2805..3743
                     /locus_tag="U4A12_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1774106.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="U4A12_001752"
     CDS             3755..4666
                     /locus_tag="U4A12_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1774107.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="U4A12_001753"
     CDS             4690..6162
                     /locus_tag="U4A12_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1774108.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="U4A12_001754"
     CDS             6213..6824
                     /locus_tag="U4A12_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1774109.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="U4A12_001755"
     CDS             6854..8767
                     /locus_tag="U4A12_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEN1774110.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="U4A12_001756"
     CDS             8895..11876
                     /locus_tag="U4A12_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEN1774111.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="U4A12_001757"
     CDS             11873..13534
                     /locus_tag="U4A12_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEN1774112.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="U4A12_001758"
     CDS             13574..14506
                     /locus_tag="U4A12_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEN1774113.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DASJOX010000062        13810 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJOX010000062 DASJOX010000000
VERSION     DASJOX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.063"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(221..1423)
                     /locus_tag="U4A12_001759"
     CDS             complement(221..1423)
                     /locus_tag="U4A12_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1774114.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="U4A12_001760"
     CDS             complement(1426..1620)
                     /locus_tag="U4A12_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1774115.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="U4A12_001761"
     CDS             complement(1667..2515)
                     /locus_tag="U4A12_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEN1774116.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="U4A12_001762"
     CDS             complement(2720..3070)
                     /locus_tag="U4A12_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774117.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="U4A12_001763"
     CDS             3665..4111
                     /locus_tag="U4A12_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1774118.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="U4A12_001764"
     CDS             4735..5727
                     /locus_tag="U4A12_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1774119.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="U4A12_001765"
     CDS             5743..6627
                     /locus_tag="U4A12_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1774120.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="U4A12_001766"
     CDS             6676..8283
                     /locus_tag="U4A12_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1774121.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="U4A12_001767"
     CDS             8284..9768
                     /locus_tag="U4A12_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1774122.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="U4A12_001768"
     CDS             9945..10937
                     /locus_tag="U4A12_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1774123.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="U4A12_001769"
     CDS             10989..11858
                     /locus_tag="U4A12_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1774124.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="U4A12_001770"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="U4A12_001770"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1774125.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DASJOX010000063        13789 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJOX010000063 DASJOX010000000
VERSION     DASJOX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.064"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(15..875)
                     /locus_tag="U4A12_001771"
     CDS             complement(15..875)
                     /locus_tag="U4A12_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN1774126.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            967..1395
                     /locus_tag="U4A12_001772"
     CDS             967..1395
                     /locus_tag="U4A12_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1774127.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1557..1733
                     /locus_tag="U4A12_001773"
     CDS             1557..1733
                     /locus_tag="U4A12_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN1774128.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            1760..2206
                     /locus_tag="U4A12_001774"
     CDS             1760..2206
                     /locus_tag="U4A12_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN1774129.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2360..3739)
                     /gene="brnQ"
                     /locus_tag="U4A12_001775"
     CDS             complement(2360..3739)
                     /gene="brnQ"
                     /locus_tag="U4A12_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1774130.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            4445..5416
                     /locus_tag="U4A12_001776"
     CDS             4445..5416
                     /locus_tag="U4A12_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN1774131.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5440..7632
                     /locus_tag="U4A12_001777"
     CDS             5440..7632
                     /locus_tag="U4A12_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN1774132.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            7649..8125
                     /gene="ybeY"
                     /locus_tag="U4A12_001778"
     CDS             7649..8125
                     /gene="ybeY"
                     /locus_tag="U4A12_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1774133.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            8109..8504
                     /locus_tag="U4A12_001779"
     CDS             8109..8504
                     /locus_tag="U4A12_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1774134.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            8519..9418
                     /gene="era"
                     /locus_tag="U4A12_001780"
     CDS             8519..9418
                     /gene="era"
                     /locus_tag="U4A12_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN1774135.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            9561..10373
                     /gene="recO"
                     /locus_tag="U4A12_001781"
     CDS             9561..10373
                     /gene="recO"
                     /locus_tag="U4A12_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN1774136.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            10757..11674
                     /gene="glyQ"
                     /locus_tag="U4A12_001782"
     CDS             10757..11674
                     /gene="glyQ"
                     /locus_tag="U4A12_001782"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN1774137.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            11676..13751
                     /locus_tag="U4A12_001783"
     CDS             11676..13751
                     /locus_tag="U4A12_001783"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1774138.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         4644 a         2478 c         2891 g         3776 t
ORIGIN      
        1 aaagacaaaa atgattaatc gcttttttct gttgtttcag ttccataata actatgatct
       61 tctaggtcta aagcattcaa aataagcgaa gccgtttctt caatagataa agatgccaca
      121 ttgatcacta cacatcctag tttttcataa agatcattcg caaaatcgag ttcagctcta
      181 attttttcga tatcggaata agcagtatca gggtttaatc cgtaagagcg catccgttct
      241 ttacggatat tatttaaaat atctgggtta ttcgttagtc ccacgatttt tttaggatcg
      301 atttcccaca gctgtttggg aatatgtgct tggggaatca aaggtaaatt agcaactttt
      361 aaatttttat ttgctaaaaa caagctcaaa ggcgttttgg aagttcggga tacgcctagc
      421 aaaacgacat ctgcttctaa aaaaccacga ggatctttcc catcatcgta ttttacagca
      481 aattccattg ctttgatccg tttgaaatag ttctcattca ataaatggag ggcaccaggt
      541 tgccgaattg gcttgatacc agttaaacgt tcaatttctg atatcggtgg cgtcatcaca
      601 tcgaaactaa ataattgatg ttcttcacaa aaagaacgtg ttatctcgat cagttcttga
      661 ttgattaatg tatgcaggac aagtgctttt tcttctaatg catcttgcaa tgcctgcctt
      721 aatgtttttt catctgtcac gaacgttctg cggaataggt tgaactcaac ggtaggatac
      781 tgagccatag tagcttgcgc tagttttgtc gcagtttctc cagcagagtc ggagatgaca
      841 aaaaaagtaa cgatttctgt agaatcggaa gacatggata aaacctcact tgcatttttt
      901 ttatgtttat tataccatac tctagaatgc gtacttcgta atcattttga tttggaggaa
      961 taagtcatga aaaaacagac attgatcgaa caatcattag aaacattgaa aaataacggt
     1021 tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg taaccgctac
     1081 gtatctgcta aagaagtttt tgactatatg aatcaggaat tcaaaggggt aagttatgat
     1141 acgatttacc ggaatctcca tgattttgca gagatcgatt tactggaaga aaccgattta
     1201 aatggtgaaa tgaagtttcg ttttcattgc tgtcaggggg aaatcggcca tcaccatcat
     1261 tttatctgta cagtgtgcgg aacaacgaaa gaaatccata tgtgtccaat gaattatttc
     1321 gaagaacagc taggtggatg cacgatcgaa gggcatagat tcgagatttt cggaagatgt
     1381 gaaaattgtc aataaggatt taaataaaat aacatttgat aatttaaaaa aaaagcaaga
     1441 tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct tgttttttga
     1501 gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga attaacatgt
     1561 caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc ttcaaacgtt
     1621 ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac gaaaaaccaa
     1681 gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc tagttctgtg
     1741 atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca tgaaagcagc
     1801 gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt catctataca
     1861 aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaactaa cggtgttgtc
     1921 tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag gacgtgacga
     1981 tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa aaatatatgc cggaacaact
     2041 ttccgacgaa gaaattcgac aacttgttca agaagcaatc actcaaactg gtgcttcatc
     2101 aatgaaagaa tttggcaaag ttatgggtgc aattatgcct aaagtcaaag gaaaagcaga
     2161 tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa tcttaatttt atcatttaaa
     2221 ctgcggacag tgattgtccg cagttttttg tgttcgtaaa aaaagaacta aaaaacagct
     2281 agaagaaaga ataagattct ttcttctagc tgtttttttc ctgtttaata tctcttatgt
     2341 taaattgtgt ttgatttggt tattcttctg gtagttcatt tgcttcttgt ttataccctg
     2401 ataggcgata ggcgtaataa aagagtaagc tgccagcgta accaagtaac gttgggacca
     2461 tccagccaag tcctaatgca gcaaaaggaa cgtgatgatg ataaaatcct agtaagcttg
     2521 ctacgacagt ttgctgactc actaatgcag gcgaatttgc caacatatcc aatacggctg
     2581 gaacggcaga aaagaacatc gttacttgat agacaaatgg actcttttgg aagaattttg
     2641 ctgttaagga aagcaagatt aacgcaagtg acaatggata caaaagcatc aatactggta
     2701 aagaccattg gatgatatta tccaatccgg cattcgcaac gacaaaagag acaaaagtcg
     2761 ttaaccgcag ccatgtcata taactgactt tagggaacag tttatggaaa tcttgtgcaa
     2821 aagaaacaac gagtcccata gcggtagtga agacacctaa tgtgaccatt acccctaaga
     2881 agctagcacc gaagttacct aaataaaaat tagtgatctg agaaagtgca gttccgccat
     2941 tttctgataa aggcatctta ttcaaactca tcgcaccgag aaggaccaga ccaaaataaa
     3001 tcatgacttc taacgcaacg ctcaggctac cagcttttgc tgttaattct gtgacttttt
     3061 tatctttaaa accaagatca cgaactgcat ggacaaaagt gacacttaaa gcaagtaaag
     3121 ctactgcatc aaccgtgttg tatccttcta aaacgccatt tgtcaaagcg ttcgtctgat
     3181 aagaatctgc agcagattgg tttagattcc ccatgggttg gaataaagaa acgacaaaaa
     3241 tgatagctaa taatgcaaga aacaaggagt ttaaatactt tccgacatat ttgaccaaac
     3301 gtgttggatg tacagtaagg aaataagcaa gtaaaaagaa aagagcagag aataaaaaca
     3361 tacctaatga atcatatttt tcaggcaaaa agggtttagc agccatttca aaagcagttg
     3421 ctgctgtccg cggtgtacca aagaatggtc cgatcgttaa atgaacaagg atcaagaaca
     3481 aggcagcata ccattttccg acaggtttag ctaagtcata cagaccatca ctttttgtca
     3541 ctactacagc taagatagct aataatggga aaagagcgcc tgagatggca aatcctacac
     3601 cagcgctgat ccagtttgat ccagccattt gacctaagtg gacaggaaag atcagattac
     3661 ctgacccgaa gaacatcccg aagataagtg aactgaccat caataattgc ttccatgaat
     3721 aagtttttgt ttcttccata aatttttcct ccaataatta tcattttgag taacaaaata
     3781 aacgttgcag aaactagaaa tgccactaat atcagattcc atgaaaacca ccaccttaaa
     3841 atataaaaaa aagccctttt ccacttggga aaagggctct agattctaga acggtaccac
     3901 cttttttcgc ttaaaaaagc ttcttcattc acggacgctt ttcgataaag ttatccgcta
     3961 gcctgataat ggaggcagcc acaatgatct acgttttcga cattgactcg gaaatgatgt
     4021 tcgtctattc gatttattgt catttctcac ttacatgact cactgggaat aaatcgctga
     4081 atagcctact gttttcctcg acgctttgct gtatatttat ttgttgacgt tataatacac
     4141 ctgttttttt tgaaatgcaa gaatatttca aatattttgt tttctttttt tgaaacaaga
     4201 aaaataccca gagaatactt gctaagttag acggaatact tgtcctctat cttatgcata
     4261 gcttacatga tcagcttttt ttattcaaac gagaccttta gagaatggaa gcagctattt
     4321 tttaacgttt cttggtactt atggttaagc aactctttta gaaccttttc ttatttgcta
     4381 tatagtttct tctgagtatg gtaaaatagg aaagaatgaa tcgaaaaaaa ggggatgtct
     4441 atttttgacg gaagagtcta gttctttaga gattagattg acggctgctg atgatgtcag
     4501 catgctttta ggtgcacacg acaagcatat aaaactgatt gaagaaacaa cagaaacaac
     4561 catccataca cgtggagaaa tcattcagat cattggcgaa caatcccaga tttccttggc
     4621 tgcctcagtc attcggacat tgcaggaatt gatcaaacga ggaatccatg taagtacacc
     4681 agatgtcgtg acggcactga aaatggggca aaaaggcaca ttagattatt ttgttgaaat
     4741 gtatgaggaa gagatcgtca aagaccgtaa tggaaaacca atacgggtaa aaaacagcgg
     4801 ccaaaagaaa tatgtcgaat cggtagccaa acatgatatc gtatttggcg taggaccagc
     4861 cgggacagga aaaacttttc ttgcagtagt attagcaatc gctgccttga aaaaaggtga
     4921 agtgcaaaaa atcattttga cgcggcctgc tgtggaagca ggggaaaatc taggctttct
     4981 tcccggggat ctaaaagaaa aagtagatcc ttatctgcgt cctgtttatg atgctttata
     5041 ccaaattttt ggattagatc atacgaaccg attgatggaa cgaggcgtga tcgaaattgc
     5101 tcctttagct tacatgcggg ggcggacatt ggatgatgca ttcgttattt tggatgaagc
     5161 acagaacacg acagttgctc agatgaaaat gttcctgaca cgtcttggtt atcaatcaaa
     5221 aatgattgtc aatggagata cgagtcagat tgatctgcct cgagggacaa caagtggtct
     5281 tgttcatgca gaacgaacat taaagcaaat caagaaaatc gattttgtta attttgaagc
     5341 aagtgacgtt gttcggcacc ctgtagttgc cgaaatcatc aaagcttatg aaaaggcaga
     5401 tctccatcaa gaataagctt tgcttaggag gataagcaca tgttgaataa aacactgcag
     5461 aaactacaag aaaaacttgg aaaggcagca ttgcctttcc tgctgttctt gttttttatc
     5521 attatctgcg gcgttacatt tagtagtgtc aaacaaaagg ccaatgattt cagagaaggc
     5581 caagtagcag aagaaagtat ccgtgcgaac aaaacgatcg agaacacgga agaaaccgaa
     5641 caaaaaagaa aactggcagc agaagcagtc actccagaat acacttacca aaaagatctt
     5701 gcagatgacc agaacaatcg gatcaagcaa ctatttgaat tgatcgataa aacgaatgat
     5761 gccatcaaca aatcttatga cgaaaagatt gataaggcaa aggaagatga aaatatccca
     5821 aagcctacta cagaagagcg gatcgccgcg ttgaagaaaa attttgaaaa tgtgaatgca
     5881 gaaaatgttg cgttttatca aaaactaccg aacaattttt attcgacgat atttgaaatg
     5941 actgaaactg agatccatac tgtgagggat gagagtctga agctcattga cgaacagatg
     6001 agcaaacaag ttcgtgaaag tgaattggaa gcttttaaac aagaagcaga agagcaaatc
     6061 caatatttga atgtcacacc agcacaacaa caaatgattc gctacttagt ggatcaaggt
     6121 atcgtagtga atgacgtatt gaatgagaaa aagacagaag aattgaaaca atccgcacga
     6181 gaagctgtac aaccagtgat gatcttccaa ggagaaatca ttgttcgtga aggtaatcaa
     6241 atcgatgctt cagctatgaa aaaattggaa ttactaggac tgactaatca aacaacttcg
     6301 atttttccgc taattgccat ggttttagct gttttgcttc aaatcggcgt actgatttat
     6361 aattcacttc aattccaaaa tgcaggagaa agaacaaaat acgtgctttt ctatgtgaca
     6421 gctatgtcag tcagcatagt gctgatgaaa tttttccaat tattccaaac ggaacaagct
     6481 acttatatcc cgctgtttta tcccgctgct tttgcaccac ttgttttaag ctttttcttg
     6541 aaccgaagag cagggatcat ggctggtatt ttccaagcag tatctgcact tttcattttt
     6601 tatgattcga ttggcacgaa ctttctaacg attatcttga tgtcttatgt tttctctggg
     6661 ctcatggcaa cgattgtcag acgcaaacgg atctcagaac aaggattatc tgctgccatg
     6721 tgggtcatta tttttcctgt gatgatggat gtcattttga tcgtctatca aggaatgagc
     6781 tttggtgatg caacgacttg gttaatgctt gtctgcgggt tcactggtgc actcttctca
     6841 ttcttgctga cgatcggtct acacccatat attgaattga tggtcaatga tgacagtgta
     6901 attgtattaa atgaactcag caatccaaat catcctttat tgaaacagtt gctggaagaa
     6961 gcgccaggta cttatcatca tagtatgatg gtagctaatc taagtgctaa cgctgtagca
     7021 gaaatcggcg gacgttcttt gctaactcga gtagcttgct attatcatga tatcggcaag
     7081 atcaaacatg ctagcttttt cgttgagaac ttgccttcag gagcggaaaa cccgcacaat
     7141 ttcttattgc cagaagacag taaacatatt atctttggac atgtgacaga tggagccaaa
     7201 atattggaag attataatat gccgcaaatg gtgatcgata tttgcaaaca gcatcatgga
     7261 accacgctaa tgcgttattt ctacatcaaa gcgaaagaac gcaatccgga agtaacagaa
     7321 gagcaattcc gttatcctgg accaaaaccg caaagtcgtg aagcagggat cgtcaatatt
     7381 gctgacagtt gtgaggctgc agtacgggca atggatcatc cgacgagcga aaaaatcgaa
     7441 agttttgtcc ataatcttat cgaagagcgt atttccgatg gtcaattaga cgatagtgga
     7501 ctgaccttga aagaaatccg aaaagtggaa aaatcattga tcagcggttt aagtagcacc
     7561 ttccattctc ggatcaaata tcctaaaatg aaaacagaag ttgagaagat aaaggaagaa
     7621 caagaagaat agaaagaagc gatgtataat ggatattacc tttatggatg aaacaaacca
     7681 agtctcagaa gagaaaatga aagaaatcga tgacttgctg caatttgcag catcttattt
     7741 aaagctgcca gaggaaacag aaatgtctgt tactttcatg gacaatgcgg caatccaagt
     7801 aatcaatcgt gattaccgtg gaaaagatgc accgacagac gtcatcagtt ttgctctgga
     7861 agaagaaggc gaagacgaaa tcccagtgat ttttgaagat ggagagaatc cattgcctcg
     7921 tgtactagga gacatcatga tttctattga acgtgctgct gaacaagcag aagcttacgg
     7981 ccattcatat gatcgggaac ttggcttttt agcggtacat ggattcttgc atatcaacgg
     8041 gtatgaccat atgacaccag aagatgagaa agaaatgttt ggtttacaga aagagatctt
     8101 ggatgcctat ggactcaaaa gataaaaacg tagagaaaaa caaacatttc ctgatgtctg
     8161 tggaatttgc tgtccaagga gtcaaaaccg tttttgagga agaacgcaat atgaggaaac
     8221 atgttgcgtt cggtatcctt gctttattta tgggcgtgat tttccagctt gatcggatag
     8281 agtggttatg gctgctttta gctgtttttc tcgtttggat agtagagatc atcaatactg
     8341 tttttgaaaa tgtagtggac atgtttacgg attttcattt tcatccgatt gggaaaaaaa
     8401 tcaaagatat ggcagctgga gcagttttag tgaccgcttg ttttgctgtc atcattgggt
     8461 tgattttatt tgtccctaaa atatatcatt tgttgtttaa ttaaaaagga gaacgtatat
     8521 gccagaacat aaatcagggt ttgtcgcaat cgtcggccgc ccgaatgtag gaaaatccac
     8581 attattgaac cgaatcgtgg gtcaaaaaat tgccattatg agtgacaagg cacagacaac
     8641 tagaaataaa atccaaggag tatatactac accagaagct cagctgattt ttatcgatac
     8701 accaggaatc cataaaccaa agcatcgtct aggagatttt atggtagaag cagcttacag
     8761 tgcgttgaaa gaagtggatg cagtcttatt tatgatcagt gctgatcaaa aacgcggccg
     8821 gggagacgat tttattattg aacgtctaaa aaacgtgcat tctccagttt atctgattat
     8881 caataaaatc gataagatcc atccagacga gttgctggga atcatcgaag attattcgac
     8941 gcagatgcct tttgcacaag tgattccgat ctctgctaca gaaggaaaca atgtcgaacg
     9001 cttaatggat gtattagtca gtgaaatgcc agagggccca caatattttc cagatgatca
     9061 agtgactgat catcctgaat actttatcgt ttctgaattg atccgtgaga aagtcttgtt
     9121 tttaacacga gacgaagtgc ctcattctgt cgcagtcgtt gttgattcga tgaagcgaaa
     9181 cgagaatgat aaaatccata tccaggccac gatcatcgtc gaaagagata gccaaaaagg
     9241 aatcattatc ggcaaaggcg gaaaaatgct gaagcaagta ggaacgaaag caagaaagga
     9301 tatcgaaaat cttttaggag acaaagtatt tctagaactt tgggtcaaag tccaaaaaga
     9361 ctggcgagac aaaaaagtct atctgcaaga ctttggctac cgaaaagacg aatactgata
     9421 ttctgaactt cttataggtt aagtatagaa ataaactgga taaaaatccg cacgttgaga
     9481 aaaacggcgg aagatcgaaa cataaataat ctggatcggc agaatctgct tttccagatt
     9541 tattttttag gagggagaat atgagccagc ttgcagaatc gaaaggcttg attttatcta
     9601 gccgtaatta caaagaaaaa gataaattag tcaaaatctt tacggaatct tctggcaaaa
     9661 tgatgtttta tatcaaagga atccatcggc aaaatcagcc attagctccg gctttgctcc
     9721 cctttaccga agcagtctat atcggtaatt ttcgaagtga aggtttgtcg tttttaagca
     9781 gtgtcaaaga cgtccatgcg tttcggaaca tccaagaaga tatctttttg agcggttatg
     9841 gtacctattt gctaaatcta gtagatgcag caatcgaaga tcatcaatat gacccgcatt
     9901 tattccaatt tacgcaacaa gctctgcagc ggatggatca aggagatgat gcagaaatca
     9961 tcacgaatat ctttgaagtt caaatactgc aacgcttcgg aatcgcgcca atttggacgc
    10021 actgtgtcgt atgcggggaa acaaaaggaa aatttgacta ttcttccaaa tctggcggtg
    10081 tgatatgcga gaaacactgg ccaatggacg aacaccgtta tcatgcagat cctcgcgctg
    10141 tttatttcgt tcgaatgttt tcggctattt cttatgataa gatcagtgga atccacttga
    10201 aagaagaaac gaagcaggca atccgtgacc tcattgacca gttgtatgat gaatatgtcg
    10261 gtattcatct aaaaagcaaa aagttcattg atcaaatgaa gtcttgggca cacgtattaa
    10321 agacgccaga gaaaaatatg cctgaaaaag agatacctga aaaagaagaa taaatgaaag
    10381 atacaacaaa aaaatcaaaa ctatttgaca aatacaggga cttccgtcta ttattaaccg
    10441 tagattaaat agatcgttca ctgagaggat ctattatata aacaagaaaa ctgcaaaatg
    10501 aatacgtaca ttgaccaaga tcagtaaaaa acacgtggtt tagcgagttc gggagagtgg
    10561 aagccggacc caaacgattt ttgaagaggc acttgcgagt acgataaatg aagctgccga
    10621 atctttattc ggaagtaggg tggaaccgcg aaattattcg tccctatgtc tgtaatcaga
    10681 catagggact ttttgtcgtt ttctttcctg tattcttatt gacagcaacg cttaaaaatt
    10741 gaaaggggca aaacagatga gtaaaaaatt gaccgtccaa gatatgattt taacattaca
    10801 aaaattttgg tcatcaaacg gctgcatgct gatgcaagct tatgacacag aaaaaggagc
    10861 aggtacgatg agcccatata cattcttacg ggcaatcgga ccggaaccat ggaatgctgc
    10921 ttatgtagag ccatcccgcc gaccagctga cgggcgttat ggagaaaacc caaaccgttt
    10981 gtatcagcat catcaatttc aagtcgtaat gaaaccttca cctgaaaata tccaagagct
    11041 ttatttagaa agcttgcgtc ttttaggaat tgatccatta gaacacgata ttcgttttgt
    11101 tgaagacaac tgggagaacc catcgatggg gtgtgccggt gtgggttggg aagtatggct
    11161 tgatgggatg gagatcacac aattcacgta cttccagcaa gtcggaggac taccttgtaa
    11221 accagttact tctgagatca cttatggact agaacgtctt gcttcatata ttcaagaagt
    11281 tgaaagtgtc tatgatctgg aatgggctga tggtgtgaaa tatggcgaga tcttcaaaca
    11341 accagaatat gaacattcca aatattcttt tgaagtaagt gaccaagact tgctactaag
    11401 caactttgac cgttttgaaa aagaagctaa acgctgtatc gatgaaaatc ttgttcatcc
    11461 agcgtatgat tatattttga aatgcagcca tacattcaat ttattagatg ctcgcggtgc
    11521 agtatccgta acagaacgag caggttatct ttcccgtatc cgcaacatgg ctcgtgctgt
    11581 agccaaaatc tttgtggcag aacgcgaaaa attaggattc cctttattga ataaaaacac
    11641 agcaaatcca gaaaacatga aggaggctga ataagatggc aaaagactta ttacttgaaa
    11701 tcgggctgga agaaatgcct gctcatgttg tcacaccaag catgaaacaa ttagaagaaa
    11761 aaacagcaaa atttttagat gaaaaccaat tgacttatga tacgatcgaa acgttttcta
    11821 caccgcgccg cttagcaatc aaagtaacaa acattccaga acgtcaagca gacagtgaag
    11881 aagaagtcaa aggaccagcc aaaaaaattg ctttggacgc agatggaaac tggacgaaag
    11941 cagctgaagg atttgtccgc ggacaaggtc tgacgacaga tgctatcact tttagagaat
    12001 taaaaggcgt ggaatatgtc tatgtaacga aatacactca cggcaaagaa gcgaaagaag
    12061 tattgacagg tttagcagat gtcattaaaa gcctcacttt cccagtaacg atgcattggg
    12121 cagaatacga ttttgaatat atccgcccta ttcactggat cgtggcattg ttagatgacg
    12181 aagtaatccc gtttgagatc ttagatgtca aaacaggcag agcctctcga ggacatcgtt
    12241 tcttaggcga agatgtgaca tttgcgcatg caaaagaata tgaagaaaaa ttgaatgaac
    12301 aattcgttat tgcttcaccg caaaaaagac aagaaatgat cgttacacaa gcaaatgaat
    12361 tagccgaaaa aaatcactgg gttattgatc tagacgaaga tttactagaa gaagtcaaca
    12421 atttagtcga atatccaact gtcttttctg gcgacttcga agaaaaatat ctttctgttc
    12481 ctgaagaagt actagtcact tctatgaaag aacatcaacg ttatttcgag gtacggaacg
    12541 atcaaggtat gctgcttcct cattttattt ctgctcgtaa tggtgatcgg gtaaaattgg
    12601 aaaatgtggt tcgaggcaat caaaaagtat tgacggctcg tttagaagat gcggaattct
    12661 tttataatga agacaagaaa gcttcaatcg accaatacgt tgaaaaacta aaaactgtta
    12721 ctttccatga aaaaatcggt agtatctatg agaaaatgca gcgggtacaa gtcattgccc
    12781 gtatcattgg cgaacaagta ggactatctg aggaagaatt gatggatctg caacgagctg
    12841 ctgaaatcta taaatttgat ctagtaacca acatggtagg ggaatttcct gaactacaag
    12901 ggatcatggg cgaaaaatat gctctgctga aaggcgagaa acctgctgta gcaacagcga
    12961 ttcgtgagca ttacatgcct atatccagtg aaggagagct acctcagtct acagtaggtt
    13021 ctgttttagc gattgcagat aaattagata gcattttttc tttctttgca gttgggatga
    13081 ttccaagcgg gtcaaacgat ccttatgcct tacgtcgcca agcatacggt gtcgttcgga
    13141 tcatcgaatc gaaaaaatgg gcctttcctt tgtctgttct gcaagaaacg attagtgatg
    13201 ttatcagtaa agatacggat cgtttcggca ttggtttatc agaagggcaa caacaggtga
    13261 tcgactttat caaaggccgt ctgcgccaat tattgacaac gaagaatatt cgtcatgatg
    13321 tcatcgaagc tgttttgaat gcagaacaaa aagatctgac aaaagtattt gcagcagcac
    13381 aattgttcaa gcagcatctt gcagatgaag acttcaaacc atcgatggaa gctttgacac
    13441 gtgtcgtgaa cttagcgaaa aaagcggagc ttgaaaaaca atcagaagta gaccctgaac
    13501 tgttcgaaaa tgaagcggag aaagaactgc acaaagcagt agaggcaatg aaaaaaagct
    13561 ttgctgacca aacgatcaac gaaaattatg atcagcttgt aagccttcgc ccactgatcg
    13621 aaaattattt tgatcagacg atggtcatgg cagaagaacc agcagtacgt aagaaccgat
    13681 taacgcaatt aagtcaaatt gctgccatgg ctttgtcgct agcaagttta gatcaaatca
    13741 tcacaaaata atttctaata tgtaaataaa ccagagaata ggcacaaaa
//
LOCUS       DASJOX010000064        13780 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJOX010000064 DASJOX010000000
VERSION     DASJOX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.065"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..476
                     /locus_tag="U4A12_001784"
     CDS             138..476
                     /locus_tag="U4A12_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774139.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(681..1256)
                     /locus_tag="U4A12_001785"
     CDS             complement(681..1256)
                     /locus_tag="U4A12_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1774140.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1988..2569)
                     /locus_tag="U4A12_001786"
     CDS             complement(1988..2569)
                     /locus_tag="U4A12_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1774141.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2752..3378)
                     /locus_tag="U4A12_001787"
     CDS             complement(2752..3378)
                     /locus_tag="U4A12_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1774142.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3400..4731)
                     /locus_tag="U4A12_001788"
     CDS             complement(3400..4731)
                     /locus_tag="U4A12_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN1774143.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4886..5287)
                     /locus_tag="U4A12_001789"
     CDS             complement(4886..5287)
                     /locus_tag="U4A12_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1774144.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5373..5579)
                     /locus_tag="U4A12_001790"
     CDS             complement(5373..5579)
                     /locus_tag="U4A12_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774145.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5674..7176)
                     /locus_tag="U4A12_001791"
     CDS             complement(5674..7176)
                     /locus_tag="U4A12_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN1774146.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7194..7649)
                     /gene="argR"
                     /locus_tag="U4A12_001792"
     CDS             complement(7194..7649)
                     /gene="argR"
                     /locus_tag="U4A12_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1774147.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7747..8364)
                     /locus_tag="U4A12_001793"
     CDS             complement(7747..8364)
                     /locus_tag="U4A12_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN1774148.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8585..9958
                     /locus_tag="U4A12_001794"
     CDS             8585..9958
                     /locus_tag="U4A12_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1774149.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10033..11145
                     /locus_tag="U4A12_001795"
     CDS             10033..11145
                     /locus_tag="U4A12_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN1774150.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11217..12854)
                     /locus_tag="U4A12_001796"
     CDS             complement(11217..12854)
                     /locus_tag="U4A12_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN1774151.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13022..13630)
                     /locus_tag="U4A12_001797"
     CDS             complement(13022..13630)
                     /locus_tag="U4A12_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1774152.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3731 a         2892 c         2461 g         4696 t
ORIGIN      
        1 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
       61 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
      121 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
      181 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
      241 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
      301 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
      361 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
      421 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
      481 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
      541 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
      601 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
      661 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
      721 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
      781 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
      841 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
      901 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcata aaagccactc
      961 attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact
     1021 gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc
     1081 atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac
     1141 ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct
     1201 cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta
     1261 atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg
     1321 ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt
     1381 attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt
     1441 tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg
     1501 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagccat
     1561 tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa
     1621 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag
     1681 ctatttttat ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca
     1741 ctaaacggtg ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg
     1801 aactattttg ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta
     1861 atacacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg
     1921 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
     1981 ctaattatta aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct
     2041 gcctaagtag tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag
     2101 caatcaacgc tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg
     2161 ccaggatatt ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac
     2221 ggtattcttc atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat
     2281 aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt
     2341 ctggctgttt tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat
     2401 gcgcatggat caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac
     2461 cttttcctgt atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt
     2521 tataaataag atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc
     2581 tttttcattt ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg
     2641 tttaaaaaag actgtgccaa attatctgac acagccctca ctataaaact gttctcatat
     2701 agctagatgt tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag
     2761 ttgctctaat acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa
     2821 cgctgcatcc attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt
     2881 gaatccactc tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc
     2941 accaagaatt actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc
     3001 tggtccaaag attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa
     3061 ttcttcgata aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc
     3121 aaacatctgc tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc
     3181 atgtgggtaa aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt
     3241 taccatttca atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc
     3301 aaagccaccg cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt
     3361 atttttatta ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag
     3421 agcttcactc attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt
     3481 tccattcata gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact
     3541 tgcccctaga atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc
     3601 ttctaatact tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac
     3661 gccttgtgct ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa
     3721 tacactgtaa ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg
     3781 atccataatg attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac
     3841 gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc
     3901 tccgcgatct gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt
     3961 tcttccagtt gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt
     4021 aaaggtgacg gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg
     4081 gaattcgatt cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc
     4141 acgtggaaga aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa
     4201 cattgatgca aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa
     4261 ttgttcctga tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt
     4321 gattggtaaa atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat
     4381 cactttttca ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc
     4441 gtccatcact gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat
     4501 gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt
     4561 gaaaatcaat gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg
     4621 tgttgacttt tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt
     4681 cttaccgccc tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc
     4741 gcttcttttt tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc
     4801 aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta
     4861 gtctggttcg atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc
     4921 tagttgcttt ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt
     4981 cttcgctgat gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat
     5041 ctaaattata aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt
     5101 tgatcatatg ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt
     5161 ctggatttgt tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat
     5221 ttggtgcctc gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt
     5281 ttttcattgt actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat
     5341 gacaagtaag gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct
     5401 actatttttc caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca
     5461 aaataaatag caatcttttc tacataatct tttatatgat tgattttaag atacatatat
     5521 accagaaagt tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt
     5581 ttttcctcct tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc
     5641 cgtccaagca actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc
     5701 agaaaaatgt ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc
     5761 atcattttct atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca
     5821 gccgtttccg tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa
     5881 gttcatccct ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg
     5941 ttcgcgtggt tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt
     6001 caactctttg atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat
     6061 caggatcttt gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc
     6121 gtgtccgctt gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc
     6181 tttattgatt acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc
     6241 aatgaatctt tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg
     6301 ctcttttaat acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc
     6361 agaagcactg acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc
     6421 ttcatttgct aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg
     6481 gatatgatta ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc
     6541 taagctattc aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat
     6601 gattttccaa ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt
     6661 gactaaatct tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc
     6721 gctttttatt ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc
     6781 ttctgaggta aagaatttta ttccattata ataataggga agatggctag cagtcagcat
     6841 aaccgcagca tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt
     6901 agctaattcg aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc
     6961 tttcagtgct ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt
     7021 tggatcggtt gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt
     7081 taccatgtct gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat
     7141 atccgatcca ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc
     7201 ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata
     7261 atgaatgtat ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc
     7321 caattcgtta ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca
     7381 gactggatac gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa
     7441 acagatggct ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact
     7501 aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca
     7561 tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg
     7621 atcagtgctt gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa
     7681 aagataagta atatttcttc atattttggt attttttact tttatttttt gttttcattc
     7741 aggttgctaa cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga
     7801 ttttcttgga tcgctgatgt aaatctcttt gtgttcttta gaagctcttt catagccttc
     7861 ttttgcgatg aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac
     7921 gtgtaagatt tgggcacaaa gaccttcttc catctttttg aatactagtt gttctttcaa
     7981 gtcttccgga agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa
     8041 gtcaggctgt cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg
     8101 caaatacttt tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata
     8161 atcaggaatc agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag
     8221 acaggctact cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa
     8281 gaatggtttt ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc
     8341 tttacgccat tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat
     8401 tatcatacct ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc
     8461 tagaataagt gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg
     8521 cggtttctat ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat
     8581 gtaaatgaca aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat
     8641 gtcttttgcg ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag
     8701 acagtttgat ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat
     8761 ttcgatcatt gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt
     8821 tttagcttca aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt
     8881 catggtccta ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt
     8941 gatgttccat attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt
     9001 cggtactttg acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac
     9061 ttcacatttt acatggaatc atatcttcct gtttcttgtg cccgttttga ttctttcttt
     9121 gattatcggc tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg
     9181 gataagcgtt ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt
     9241 gggaacagtt tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta
     9301 ctatcgaagt aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac
     9361 attccgcttg tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt
     9421 tgtcttgccg aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc
     9481 gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga
     9541 tcaatatggt ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct
     9601 agcattattc ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt
     9661 ttatatgatc ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact
     9721 gaatgaagaa ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg
     9781 cgctgtggct acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca
     9841 taactatgaa gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt
     9901 gcttctcgta agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat
     9961 tccatatacg gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa
    10021 ggaggctttt ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgactcta
    10081 gccagttact tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag
    10141 gcaactaaag ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa
    10201 tggtatcgtc tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa
    10261 cttgatcaaa cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta
    10321 gctgaattgg ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc
    10381 gaatggcttt cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca
    10441 ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag
    10501 cgtttagcac aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc
    10561 gattcttggc tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca
    10621 cctggcccgg cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg
    10681 caaaaagggc agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt
    10741 gctatgcttg acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt
    10801 gctacccatg aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga
    10861 ttgactaaga gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac
    10921 gagttccgtg taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg
    10981 ccagatgatc caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt
    11041 ccttatatcg gctatctatt agccaacttc ttaaatactg gtcattggtc tacggaagat
    11101 tatcaaaagg acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc
    11161 aagtaaggat cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag
    11221 ttcaatttct gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt
    11281 cctatctgag cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta
    11341 taggacgtat cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca
    11401 aatggaccaa acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat
    11461 gtcgtttcag gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct
    11521 gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca
    11581 cggaaaggat tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata
    11641 ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg
    11701 tcattgatct tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg
    11761 atcttttcgc taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc
    11821 ccatttccgt ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc
    11881 acgcccacat tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct
    11941 tgtgttttat caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata
    12001 acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta
    12061 atcgttgatt ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta
    12121 ctagcatatt gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc
    12181 caagcaagcg tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacagcc
    12241 gctgtattat ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat
    12301 tcgggatcac aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc
    12361 tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca
    12421 aatccttctg gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt
    12481 acttttgtat ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc
    12541 tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt
    12601 cccacaatgc ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct
    12661 tcagcatgtg agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga
    12721 taagctgtct tcctgtcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca
    12781 atcgatcgaa tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc
    12841 tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac
    12901 attattcgag tttttatttg tactgctgcg ctgtgggaag caaacgagtt atttttcaca
    12961 caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg
    13021 cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag
    13081 atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt
    13141 atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc
    13201 ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag
    13261 ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg
    13321 ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag
    13381 aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga
    13441 tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga
    13501 tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag
    13561 ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa
    13621 atgaattcat attaaactcc tttttttctc cgttttttat tctttcgacg ctattaactt
    13681 tatcatttag aaatatttca taggtaaaag aaacatgaca tttaatgaca aaataacgaa
    13741 ttatttaata tttgtaacat ttaaacaaat atttttttga
//
LOCUS       DASJOX010000065        13663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJOX010000065 DASJOX010000000
VERSION     DASJOX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.066"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..535
                     /locus_tag="U4A12_001798"
     CDS             17..535
                     /locus_tag="U4A12_001798"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1774153.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="U4A12_001799"
     CDS             663..1346
                     /locus_tag="U4A12_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN1774154.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="U4A12_001800"
     tRNA            1655..1728
                     /locus_tag="U4A12_001800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="U4A12_001801"
     CDS             1887..2246
                     /locus_tag="U4A12_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN1774155.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="U4A12_001802"
     CDS             2369..4000
                     /locus_tag="U4A12_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN1774156.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="U4A12_001803"
     CDS             complement(4062..4703)
                     /locus_tag="U4A12_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1774157.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4889..4965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="U4A12_001804"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="U4A12_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN1774158.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="U4A12_001805"
     CDS             5320..5664
                     /locus_tag="U4A12_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN1774159.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="U4A12_001806"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="U4A12_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN1774160.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6404..9211
                     /locus_tag="U4A12_001807"
     CDS             6404..9211
                     /locus_tag="U4A12_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1774161.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="U4A12_001808"
     CDS             9402..9893
                     /locus_tag="U4A12_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774162.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /gene="manX"
                     /locus_tag="U4A12_001809"
     CDS             9996..10985
                     /gene="manX"
                     /locus_tag="U4A12_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN1774163.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="U4A12_001810"
     CDS             11018..11821
                     /locus_tag="U4A12_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN1774164.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="U4A12_001811"
     CDS             11842..12756
                     /locus_tag="U4A12_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN1774165.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="U4A12_001812"
     CDS             12839..13213
                     /locus_tag="U4A12_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN1774166.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="U4A12_001813"
     CDS             13352..13597
                     /locus_tag="U4A12_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1774167.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         4348 a         2277 c         2968 g         4070 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat tta
//
LOCUS       DASJOX010000066        12943 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJOX010000066 DASJOX010000000
VERSION     DASJOX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.067"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="U4A12_001814"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="U4A12_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN1774168.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="U4A12_001815"
     CDS             complement(1195..3036)
                     /locus_tag="U4A12_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1774169.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="U4A12_001816"
     CDS             complement(3218..5533)
                     /locus_tag="U4A12_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1774170.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="U4A12_001817"
     CDS             complement(5718..6578)
                     /locus_tag="U4A12_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN1774171.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="U4A12_001818"
     CDS             complement(6637..7158)
                     /locus_tag="U4A12_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN1774172.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="U4A12_001819"
     CDS             7436..8197
                     /locus_tag="U4A12_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1774173.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="U4A12_001820"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="U4A12_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1774174.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="U4A12_001821"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="U4A12_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1774175.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="U4A12_001822"
     CDS             12557..12913
                     /locus_tag="U4A12_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1774176.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DASJOX010000067        12896 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJOX010000067 DASJOX010000000
VERSION     DASJOX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.068"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U4A12_001823"
     CDS             133..3660
                     /locus_tag="U4A12_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN1774177.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U4A12_001824"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U4A12_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN1774178.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U4A12_001825"
     CDS             7448..7789
                     /locus_tag="U4A12_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1774179.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U4A12_001826"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U4A12_001826"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN1774180.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U4A12_001827"
     CDS             9146..11566
                     /locus_tag="U4A12_001827"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN1774181.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4A12_001828"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4A12_001828"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN1774182.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4379 a         2240 c         2737 g         3540 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataa
//
LOCUS       DASJOX010000068        12734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJOX010000068 DASJOX010000000
VERSION     DASJOX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.069"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U4A12_001829"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U4A12_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1774183.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="U4A12_001830"
     CDS             complement(322..891)
                     /locus_tag="U4A12_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN1774184.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="U4A12_001831"
     CDS             1077..1979
                     /locus_tag="U4A12_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1774185.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U4A12_001832"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U4A12_001832"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN1774186.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U4A12_001833"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U4A12_001833"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN1774187.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U4A12_001834"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U4A12_001834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN1774188.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="U4A12_001835"
     CDS             8547..8732
                     /locus_tag="U4A12_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN1774189.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="U4A12_001836"
     CDS             complement(8794..9990)
                     /locus_tag="U4A12_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1774190.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="U4A12_001837"
     CDS             complement(10160..11596)
                     /locus_tag="U4A12_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1774191.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="U4A12_001838"
     CDS             11889..12698
                     /locus_tag="U4A12_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1774192.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DASJOX010000069        12734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJOX010000069 DASJOX010000000
VERSION     DASJOX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.070"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(80..325)
                     /locus_tag="U4A12_001839"
     CDS             complement(80..325)
                     /locus_tag="U4A12_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774193.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(319..720)
                     /locus_tag="U4A12_001840"
     CDS             complement(319..720)
                     /locus_tag="U4A12_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1774194.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISVQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(892..2700)
                     /gene="uidA"
                     /locus_tag="U4A12_001841"
     CDS             complement(892..2700)
                     /gene="uidA"
                     /locus_tag="U4A12_001841"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN1774195.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(2728..4494)
                     /locus_tag="U4A12_001842"
     CDS             complement(2728..4494)
                     /locus_tag="U4A12_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN1774196.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(4507..5301)
                     /locus_tag="U4A12_001843"
     CDS             complement(4507..5301)
                     /locus_tag="U4A12_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1774197.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(5312..6688)
                     /locus_tag="U4A12_001844"
     CDS             complement(5312..6688)
                     /locus_tag="U4A12_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1774198.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(6817..7464)
                     /locus_tag="U4A12_001845"
     CDS             complement(6817..7464)
                     /locus_tag="U4A12_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1774199.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(7548..8975)
                     /gene="uxaC"
                     /locus_tag="U4A12_001846"
     CDS             complement(7548..8975)
                     /gene="uxaC"
                     /locus_tag="U4A12_001846"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1774200.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(9049..10128)
                     /gene="uxuA"
                     /locus_tag="U4A12_001847"
     CDS             complement(9049..10128)
                     /gene="uxuA"
                     /locus_tag="U4A12_001847"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1774201.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10131..11762)
                     /locus_tag="U4A12_001848"
     CDS             complement(10131..11762)
                     /locus_tag="U4A12_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1774202.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            12085..>12734
                     /locus_tag="U4A12_001849"
     CDS             12085..>12734
                     /locus_tag="U4A12_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1774203.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK"
BASE COUNT         4031 a         2519 c         1683 g         4501 t
ORIGIN      
        1 tctgtttaaa agaatctact aatcctttaa ctattagaag attcttttaa acagaataag
       61 tggaaagggg actttttagt tagttaattt taaattttaa gatcgcttct atcaatttaa
      121 tttctagctg tcttagcata tactcatcta cataggtaat aaattgcccc gcctcattaa
      181 atccatttgt caaacataac ttagctatat aactatgata atgtttgagt atcatcttga
      241 ttgaaattgg atcttcttct agagcagatt ggataatgga tagaggaact aatggatact
      301 gccgtttttt ggggttgtct accataagtc ataccccatt tttaattgtt caactgcctt
      361 ctttcgttga taccagatac cacttgtact catattgagg attttaccga tttcagtatc
      421 attaaaacct gcaaaataat aaagtaggat aacctttctt cttatttccg ataaatgatc
      481 aatggctgcc gataactttt gaactgaaat taatatttcc attccaaata agaaatagac
      541 atcttgttca tcaaaatgag tttgggagta cttaccttca tttgaattcg tcaatggttc
      601 ctcattatct gttatgttaa tgtttcgttt tcgaatagac ttagtttcgt ttcgtaatac
      661 ttttttacag taggcatcaa attgatgttg tatagatttc tcattgtaat tttgttgcat
      721 gataaatatg ctcctttttt cccctttcca ctaatacaac aatcagaaag cgcctttttt
      781 tcgaaaaaac tagaaaaaaa tatccttctt taaacagaaa atctttttat aaaaaaaagg
      841 agttggcaat taagcagcca ctcctttttt atattcctta taaaaggtaa attatttttt
      901 ataactgaaa tttggtattg atgaccatcg tttctttaga tagtgagcaa tcattttagg
      961 ctttctatct ctagtaaaga ttcctttctt atttccttgc actcttaaaa tcccttgact
     1021 tgtttcaaaa tctgcaaaat tccacacttg ttcaccaaca aaattgtcac atttatctat
     1081 tacttcgtga tttgcttcgt aataacccac ttgatactct tctgtaaaca ttgttggtac
     1141 tgtatcatga agtccagcta ctgtatctgc tccatattct gtaaacatta ttggcgtatc
     1201 gggacatctt tccatccatc catctagctc ttttttcaac agatattttg catgttttag
     1261 gtctccacct gaaacatacc aaccataata gcgatttaaa cataaaacat cgacaatatc
     1321 tcctacttta caaatatctg gtgttgactt taagtaagta acgattgtta caggtctctt
     1381 ctgagggtca agttctttag tcaattcaac taaaggttca aaatattctt tagcgccttc
     1441 agaatccgaa tctggttcat ttgcaataga ccacattacg acactcggat ggttcttgtc
     1501 gcgttctatc aattccttaa tcgccatttt gtgagcattt tctgtccgta tttctttcca
     1561 agtatcatgt ttttctacgt tgtcatctaa taaagtagcc atgaaattca aatgtaatcc
     1621 tactgcaggt acttcatcaa taactacaat accttctcga tctgctaagc gcataatttc
     1681 ttctgaataa ggataatgtg aagtcctaaa tgaattagca ccgatccatt tcattaaatt
     1741 aaaatctttg atatttactg cttcattcaa accacgacca ttaatatacg tgtcctcatg
     1801 cttcccaaat cccttaaagt aaaaaggttt attattaatc aaaaacttcc cattcgaaac
     1861 ctgaatacta cgaatgccaa attcttctgt gtacgtatca atcactttat tttcgttaat
     1921 taaactaact ttcatactat ataagtaagc atttagaggc tcccaaagtg ttgggttctt
     1981 aagttgaatc actccttctt tccccttagc ttctgctata agttgagctt cttcgtcata
     2041 gattgctatt agaatttctc cggtccattc tgtatcgata ttaaattcta catttgcatc
     2101 tgaatcaatt atttttgtat taatagtgac atcctttata tagtctttcg gagttgtata
     2161 caatttaacg ggtctattaa tgcctgcata attgaaaaag tcaaaattaa ccaaattttc
     2221 tttttttatg tctccatctt cctgtatcgt ttctttcaaa atccctacag gtaaagtgct
     2281 atcgtcaata acattgttta cagccactgt aattctgtta cttccatctt ttataaactg
     2341 attaatttct gattcaaatg gcgtaaaccc tcctttgtgt tgattaatca attttccatt
     2401 caaaaatact tttgcttcat gtgttgcaga tccaaaacga agaactaatc tttcttctaa
     2461 taatttttta ggaagaatca cttctttttc ataccacacc catccaacat gatttctaat
     2521 gttctctgtt tcaaataaat cattataaga agaaggaact gaaattggat aactctcttt
     2581 ttcaaccatt ggttctaaag caagttcttc ttgtaagtca tcatttgtat ctaacttaaa
     2641 tctccaaaga ccgcttaaat caattacttg cctcgtctct gtcataatgg gatataacat
     2701 gtaaatcact cctatataat ttaaatatta aataaatgta tcctttaacc cacaataagc
     2761 atctattgga cttattccct taaattcaga aaggcccatt agtttgtcca ctaatatttc
     2821 taaagtttct tgttttccat cataagtatt gatataagtt tttacttgag gcacatcagc
     2881 taaatgaaac ggacactgta cagatacaaa aattgttgga atttcatgaa tataaaatgg
     2941 aatatccgga gtacctttac ttgctggcca aacaattctt tgaactgtac ctgggttaac
     3001 attagctata ttaataatca agtcatattt ttcagttaaa tctttgattg gtctcttagc
     3061 agcatatatt ccagctatat ttttaatttt ttcttcttgc gataatcctt tcaattcttc
     3121 ttcaggtgaa atgtattttt caacttcaaa tccttttgct gacaaaatct gtttcaataa
     3181 atcaatggcc tgaggtctat ttccagcaac tacttcacca aacccacctt ttaccccatg
     3241 tacatctacc agtaaaatcc ttttatattt ttctggtaca ataggccaaa tatcttgttt
     3301 atctttaact agtgtaattg cttgatcagc tacctcttta aaaattgatt tattttcaat
     3361 taaaccaatt ttttccatag cttcttgttt cggtaacaaa attttattct tttctttttt
     3421 atgcaaacct agtttagcct ttaagcctaa aattcttctt aatgcatcac gtaaacgctc
     3481 ttctgaaata attcctttgt aataaccttc catcatataa ttaaagtctt catctggatc
     3541 attaaaaaat aggaatagat cacatccagc cgcaatagaa gtgggcaata attctgaccg
     3601 tttcattgcc ccagtcattc ctaacatatg actagcatca gtaactacta atccattaaa
     3661 cccgagtttt cctctaagta agtcagtgat taattctttt gataaagttg caggaagtaa
     3721 atcttcatct ttcagatcaa gattaaaatg ttttgaataa gctggcatat ggatatgacc
     3781 tgccataatt gaaggtaatc cagcttctat taagcctgag tatacttttc caaaagaatt
     3841 atcccattct tctgtactta aactattaat actaaaagat agatgttgat ctcgttcgtc
     3901 aataccgtca ccagggaaat gtttagctgc aggcaaaatt ccactctctt gaataccttt
     3961 catataagct aaactaagtt ctaaaacttt atcagggctt tgtccaaaag atcgactaga
     4021 aatgatagga tttctccaat ttctattaat atctacaata ggagcaaaac tccagttaca
     4081 tccaatagcc gatgcttcaa ttccagaaac tctccctaat tcataagcat atttcgcatc
     4141 atttgttgca ccaattttta cttctacacc aacttcggta ccatctgtac aagctccgtt
     4201 accacctgct tctgtattag cagcaattaa taaaggaatt ttgctttctg tttgtaggat
     4261 atagttttga tcatatacct catcagcagt tcctggatta tatcgtacag ctccaatatg
     4321 gtactgattg accatactac ttaagtaatt tttatcacga ctagatccca tattaacaaa
     4381 taattgacct attttttctt ctaaagtcat agcatcaatc gtctcttcaa tccaagaaat
     4441 agcatcttca tctaaatgat atggtttttt tcgtaagtct acttttatag tcattttctt
     4501 tctcctctac ttattagtaa gatttttcca aaaagtatct atatattttt gttcaatatc
     4561 tatatactct cccgataaac atttaaataa aatatcagtt cttaagtttt cccatgattc
     4621 tttttcttta ttaaccaaaa taggataaaa gaaaacttta ttcttatttg cagcttctag
     4681 atcaccagga gaatcaccaa tcattaatat cttatccaaa tcacaaccat tttcaattac
     4741 acttcgaata atgtcttctt tttttccttt atcttgacaa cacagttcat caactaagga
     4801 taataattca tgtctttccc attcttcttg aatagcttcc ttattagctg agcttacaat
     4861 aattatcttc ccatattttt tcagagcttt tatagtatct ctagcactta aaaagggttt
     4921 atctaatcct ttagcttgac taatctgtcg attgacttct tccgaccatt ttaaagcttt
     4981 taataaatcc tctgactgag agtattctat ttccctcttc aaagatggat tagacaattc
     5041 ttttgttaca gatacccagt gctttaatcg attaatcgat ggtacatcat ttttttctaa
     5101 tgttttaact aaacctaaaa atctgtttat tccacgagtc tctgaatata aattaatatt
     5161 attccaatcc tgtaggaatt tttccctatg atgaacatca aaaatctctg cagcaattgg
     5221 cccgaaaaag agattgtgtt tataagtcat tgtatccatg acacaaccat ctgaatcaat
     5281 acaaaatatc caactgttca tgttttttct cctagttatg tttttgattt agcaaattat
     5341 aattttctaa tactttttct ttagataatg gatatacgaa aaataaaatc aatcctacaa
     5401 caaagaaaca aactgctgga atcaacgttg aagagttata cagcttttgt aaaacttctc
     5461 cactttgtac ttttgctaac gaatcatacc ctattataga aagagtccaa cctccgacac
     5521 cgccagctaa agcttgtcca atttttcggg caaaagaata tactgcataa atagttccat
     5581 cttctctttg gccagatttc acttcttgat catcgattac atcagtaata ttcgcccaaa
     5641 tgactgtatt aaaaatacct aaccccatat atccaacaga agtaagaact aagaatacat
     5701 acatattact aggctttatc aaaaataaaa gtaaatatac aatagcagag aagaacatcc
     5761 ctactgacgc caattctttt ttaccgtatt ttttactgaa aattaaactt acaggtgaaa
     5821 caagtaccaa catcaaaata ggattgataa agttcattaa aactattccc tgtgcattat
     5881 tataataatc tggaaataca taattgttca tactcattgt taaaagctga gcaagcaaca
     5941 aaaaaatagc tgcagctatt atagaaagca acgctctatt tttagctaat ccttttaaag
     6001 atttcaacaa tgaaactttt aactgcgatt catcactttt aacccgttct acagttaatt
     6061 tataacatat tacgtaacaa ataactgcac acactgaaaa tactcctgcc acaattgtaa
     6121 aagttgatcc accacgtaca atttgagctc cagaactatc tcttgtataa ataaaaattg
     6181 gtgttaacac gccaatgaac atacctgcta aggtagctcc tatactacga aatgttgata
     6241 acgctgtcct ttcttctgca acagaagtga tagctgatgc cattgaaccg taaggaatat
     6301 taatagatgt ataaaaaata cttccccata gtatatatgt tacatacata tatataactt
     6361 taatactcat agaagctgat gccattgatg tttgatacat taaaaaactt gctaaagcga
     6421 ctggaccact catccgtaaa atccacggtt taaatttgcc agttttagat ggttttgatc
     6481 catccacaat acgccccatt gttatgtctg taactgcatc aataaatctt gctactaaaa
     6541 ataatgttcc tacggtagct ccgcttatcc ccaatacttt tgtataaaaa atcattaaaa
     6601 aagaactagc aaaaatgaaa gtgaaatcat tcccaaaatc tccaaacata tatcctattt
     6661 tgtctcttag accaaatttt ctttccatat aatttgcgct ccttcctatt gttaagcgtt
     6721 ttcattctgt tgaactgcca acttacgtac tatactctga agtataaaga gttagtattt
     6781 attttttaca ttttatagta ttatttattt tttctcctaa taattcttta tctcataaaa
     6841 cttatctata tatttactag ctattcgagt tacttcttca aaatcacctt tttcaacagg
     6901 agccaacaaa ttcccaccta caccaactgc aacaacgcct gcttcaaacc actcttctaa
     6961 gttatctaga cttactccgc cagtaggcat tatatttaca tatggtaatg gagcttgata
     7021 cgtttttaca acagatggac caaaagcaga acctggaaat agtttaatga tttctactcc
     7081 agcggtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat aaggaatttg
     7141 atataaatta caaatctctg ctgtttgtcg atcaaaagat ggacttacga tatattctgc
     7201 tccagccata attgcaatac gagcagtaaa cgcatctaaa actgtaccgg ctccaattat
     7261 tacatctttt tgatcagcat attttttagc aatttctttg attatttctt cagcattcgg
     7321 aactgtaaaa gttagttcaa tccctttaat tccacccgca gtaattgcat ttattgcttt
     7381 aatggcctca tctttatttt ttccacgtac aaccgcaatg actccagccg cttctaactt
     7441 acttaaaatt tctacttttt tcatatctat ctccttcttt tatggataca tactctagct
     7501 agttgtacta acttacaata aataaattta agcttttatt ttttgtatta ttttctactt
     7561 tcgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt ctccactatc
     7621 tgactaagat acatttcatc atctggaatt cgacctgctt taacccactc gcttagtaat
     7681 tgacataata ctcgacggaa atactcatgg cgaggatagc ttagaaaact tcgagaatca
     7741 gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgcg ccatcgtagt caactgttga
     7801 cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat tttttgaatc
     7861 ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcggt tggatttaat
     7921 gaatataaaa ttgtttttgg taagcaccca ctcttttcag cagtgtcgaa caatgctaca
     7981 acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc tactccaatt
     8041 tgttccttta atatacttga attatttctt aaagcattta aatgcatttg catcgtccaa
     8101 ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt
     8161 tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt gtcgagtatt
     8221 ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa tcgacagcca
     8281 atttgatgaa aataatccac tcgttggctc aatgcctgct tcaacccatt aaagtttttg
     8341 attggtatac ccgctgcttg actaagtttt aaaatatagt tcgaaaatcc tgatgatttg
     8401 atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt atcctgttta
     8461 gaaagctcag tatgatatcg caagtcatct gtaggatcat ccgttgtaca aatggctttc
     8521 acatccatca tcttcagcaa ttcttttggg cgataggaat ccgtttggat tttttcgttg
     8581 gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa
     8641 cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt ctccactgtt
     8701 tttacaaaag ctaaaaattt ttcataatca ttacctgcac cactgatcaa ctcttcttct
     8761 atcccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt atcatttaac
     8821 catacctgag tcaaattctt gtattgctta ttttctagca aacatgctgg gtctaaatga
     8881 caatgataat cgatgattgg ttggtgttct gcaaatttat gaaaaagtct tttagctatt
     8941 tcattctcta ataaaaagtt cttatctaaa aacatattta agcactcctt tccttccctt
     9001 agtttttaga agagaaaatt tttcttctaa atagactcca ttatataatt atttactcgc
     9061 ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag ctcggtcata
     9121 taacccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat
     9181 atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc
     9241 atattctgat ttatgcggcg cttcataaaa atcgagtcca ttatcactta aatatttgat
     9301 attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata
     9361 gacatcatta tctggtcgtt ctgcaatact tccagtacat aacgtaatac cattagaagg
     9421 agaatccact acattacaca accattctaa atcgtcacga tttttaacaa tacgaggtaa
     9481 tccgaaaata gaatatggtg gatcatctgg atgaactgcc attttaatcc caacttcttc
     9541 acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgcaatt tcgcttcatc
     9601 aacttcttta taggcttcta ataattgctt gatttgtgat aaccgatgag gttcccaacc
     9661 cggcaattca aaattattag tagaattttc tacttttcta ataatttcct ctggatcagt
     9721 aggaatattt tttgaaatat aggctaacgt agaagaccca tcatctaaat gataatccaa
     9781 atctgatttt acccaatcaa agactggcat aaaattataa cagataacct caatcccaaa
     9841 ttctgccaaa ttgcgaatcg tctgtttata attctcgatg tattgatctc gtgttggcaa
     9901 accaattttg atatcatcat gaacattaac actctcaata actttcaagg taaggccatt
     9961 ggcttcgact tcatctttta aagctttaat tcgttctctc ggccagacct ctccggctgg
    10021 tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag
    10081 tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct
    10141 ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt
    10201 tcgcaaaaga ttttttcaat tttctttcct atctcaactt gatataaatc taaaccaaat
    10261 atttccttat tttctaagat ttcctgtacc gcacaatgaa tctttgcagg atcttgttca
    10321 cctaatttca atactgagaa tttttcctgc aactctacca ataaaggatc aggacttggc
    10381 acaaaatttt ttccttgatc atcgactgcc aataaataac gtaaccaagc agcaatagcg
    10441 ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt
    10501 tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg cggtgtatct
    10561 ggaatatagg gattaggcaa acgtttattg attacttcat caataaattg ttttggatca
    10621 ataacgcctg gactttcaac tactggtaag ccttctacat aacctatccc tttaattaag
    10681 ccaaccaaat catcatcttg catttctgcc tgaattgatt tataccccaa caaacaacct
    10741 gttattgcta atgccgtatg gagcggattt aaacatgtcg taaccttcat gatatctgct
    10801 ttatcaactg tttctctatc tgtcataatc acaccagccg cttccagttt aggtcgacca
    10861 tttggaaaac tatcttcaat gactagataa tggacttttt cagtattggt aaaagcagcg
    10921 atattggtat ttttcgcagt atgaattata tctaagtctt cgaaacccgc cttcaataaa
    10981 tccattttga cactttccga aggatttggc gtaattctat caatcatact ccaaggaaaa
    11041 gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacgta tccttttttt
    11101 tgccaagctt ctgcaatcat aaaaatgcta tcttgaaaac gttgaccatt ctgtgagaaa
    11161 ttatcagtag aaaccatcgc aatcggtaac tctccagcat taaacctgct atacaacaag
    11221 gatgtcacaa tactcatcgt atgttgagga ttggtaggtc cagattttat atctgcttcc
    11281 actaccgaaa aaaattgtcc tttagaatct tttaaactgt atcctttttc agtaatagta
    11341 aacgttgtaa actgtaatgt aggatcttca aaatatttaa ccactctttg atagtccgtt
    11401 atattctttg gattgcaata taaactatca gctgttactg ctaaaagtct ttttttgagt
    11461 tcaccatctt cacccataac aatctccaaa atatcattgt tgtagtcatg ataagctttt
    11521 tctactactt cttcatcaaa tgtctcacac acaactactc cggattctaa ttcattttga
    11581 tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa
    11641 tgaagccaca ctggatgttt ctgaccttct tgtttgaccg cttcgacatt aaaatacggt
    11701 agttgaactt gcattttgtt caatccttcg atagaatctt gccagtcttt aatcaaaacc
    11761 atatttaccc ctccatactg atatgttagt ttccgttaca attgtagtat aaaatcgaat
    11821 gtaatcgctg tcaataagaa aaaactaaaa atattctttt ttctaccatg tcagaattat
    11881 tgtgtctttt tttagtaaaa gcacaccaat atatattttt tagaactaac ttaaattaaa
    11941 gcttaatttc tctttgatct tatttgagta ttttttgttt ttttaatagc tagtatgtta
    12001 ctttgaaatt tgatattata taacgagcat agaactaaaa tatcaaattc aaaaatgaaa
    12061 ttttgagagg gattaaatat gacaattaaa actagtctcc aaaatcaagc ttatcaatct
    12121 attcgtcagc aaattatcta cgctgactta gaacctggaa aaaaagtatc agaaaaaaaa
    12181 ttggaagaaa cgctttctat cggtcgtact ccaatacgtg aagcattgat tcaactccgt
    12241 catcaaggac ttgttgatac aattcctcaa agtggaaatt atatctcatt aatcgactta
    12301 gatttagcaa aaaatgctcg ttttgtcaga gaacatttag aacgacaaat tatgctagag
    12361 tgttgtgcca agatgaataa tcaaaagaaa caagttttag aaactataat tgcagaacaa
    12421 gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc ataatgataa tttatttcat
    12481 aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc aatggttgga agataataat
    12541 acgcacttgg aacgatttcg ttggttacga gtaaaaacgg aaggattaaa atgggaaaaa
    12601 attatggaac aacatcatca actatttaaa gccttgacca tgcaagaccc tgaagaagct
    12661 aacttcttaa catccttgca tcttcattta atgcttgatg aacaaaatgt tgtattgact
    12721 actcacccaa aata
//
LOCUS       DASJOX010000070        12574 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJOX010000070 DASJOX010000000
VERSION     DASJOX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.071"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            65..1948
                     /locus_tag="U4A12_001850"
     CDS             65..1948
                     /locus_tag="U4A12_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1774204.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2023..2307)
                     /locus_tag="U4A12_001851"
     CDS             complement(2023..2307)
                     /locus_tag="U4A12_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1774205.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2297..3031)
                     /locus_tag="U4A12_001852"
     CDS             complement(2297..3031)
                     /locus_tag="U4A12_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN1774206.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3160..3783
                     /locus_tag="U4A12_001853"
     CDS             3160..3783
                     /locus_tag="U4A12_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN1774207.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3975..5096
                     /locus_tag="U4A12_001854"
     CDS             3975..5096
                     /locus_tag="U4A12_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN1774208.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5103..8231
                     /locus_tag="U4A12_001855"
     CDS             5103..8231
                     /locus_tag="U4A12_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN1774209.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8250..11444
                     /locus_tag="U4A12_001856"
     CDS             8250..11444
                     /locus_tag="U4A12_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN1774210.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11590..12297)
                     /locus_tag="U4A12_001857"
     CDS             complement(11590..12297)
                     /locus_tag="U4A12_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1774211.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4281 a         2211 c         2648 g         3434 t
ORIGIN      
        1 gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata
       61 ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct
      121 ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg
      181 cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct
      241 gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat
      301 gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt
      361 gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt
      421 caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc
      481 accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat
      541 cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt
      601 aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc
      661 aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct
      721 tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat
      781 tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga
      841 tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt
      901 gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt
      961 atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat
     1021 tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga
     1081 aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc
     1141 acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat
     1201 cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg
     1261 tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat
     1321 cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc
     1381 ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca
     1441 aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca
     1501 agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt
     1561 accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgattggact
     1621 aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga
     1681 tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa
     1741 tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag
     1801 ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg
     1861 gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa
     1921 cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg
     1981 tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag
     2041 caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa
     2101 tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt
     2161 aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc
     2221 taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa
     2281 tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc
     2341 catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt
     2401 tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga
     2461 tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag
     2521 gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca
     2581 cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg
     2641 ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat
     2701 tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat
     2761 ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga
     2821 tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg
     2881 cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag
     2941 catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata
     3001 attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc
     3061 gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt
     3121 tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc
     3181 ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa
     3241 gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca
     3301 ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg
     3361 ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga
     3421 gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg
     3481 agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca
     3541 ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca
     3601 cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta
     3661 tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca
     3721 gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa
     3781 taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac
     3841 tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt
     3901 aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt
     3961 tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac
     4021 aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga
     4081 aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag
     4141 ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga
     4201 tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc
     4261 catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag
     4321 tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat
     4381 tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata
     4441 aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa
     4501 tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc
     4561 ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg
     4621 ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga
     4681 tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta
     4741 gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg
     4801 atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata
     4861 gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga
     4921 ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga
     4981 aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg
     5041 caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa
     5101 gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag
     5161 atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca
     5221 aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc
     5281 gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt
     5341 tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag
     5401 caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg
     5461 atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca
     5521 gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag
     5581 aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat
     5641 tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc
     5701 aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag
     5761 tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc
     5821 aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa
     5881 aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag
     5941 aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg
     6001 aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc
     6061 gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa
     6121 agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg
     6181 tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg
     6241 tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat
     6301 tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa
     6361 gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg
     6421 cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa
     6481 aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa
     6541 caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat
     6601 gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg
     6661 tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag
     6721 atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc
     6781 aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag
     6841 aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg
     6901 ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa
     6961 tcgaacaatc tttagataga cgaaaaagca agatggccaa tgaaagaata gagctggaag
     7021 aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga
     7081 cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat
     7141 tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag
     7201 aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc
     7261 ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa
     7321 aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa
     7381 caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta
     7441 cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa
     7501 atatccagct aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa
     7561 taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta
     7621 gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag
     7681 atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga
     7741 ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg
     7801 atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca
     7861 gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc
     7921 gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag
     7981 ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg
     8041 atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa
     8101 cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac
     8161 gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc
     8221 aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg
     8281 cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag
     8341 aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta
     8401 cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc
     8461 atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag
     8521 accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt
     8581 tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc
     8641 aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg gcactttact
     8701 tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt
     8761 ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc
     8821 tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg
     8881 ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa
     8941 gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta
     9001 cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt tctgatcaaa
     9061 acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga
     9121 atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt
     9181 ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg
     9241 agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc
     9301 ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag
     9361 atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag
     9421 tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag
     9481 aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca
     9541 gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag
     9601 aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac
     9661 ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag
     9721 taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag
     9781 aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg
     9841 gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat
     9901 tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga
     9961 tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg
    10021 tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa
    10081 cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt
    10141 ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt
    10201 tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct
    10261 taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa
    10321 tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga
    10381 cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt
    10441 tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga
    10501 gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag
    10561 aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat
    10621 tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag
    10681 ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac
    10741 ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca
    10801 catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa
    10861 gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg
    10921 gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt
    10981 tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag
    11041 ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag
    11101 acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg
    11161 gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca
    11221 ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt
    11281 ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac
    11341 gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag
    11401 aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg
    11461 ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata
    11521 gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat
    11581 ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat
    11641 ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc
    11701 ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa
    11761 ggtctcttct ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg
    11821 tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa
    11881 ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa
    11941 tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat
    12001 attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg
    12061 agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt
    12121 ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata
    12181 tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa
    12241 tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat
    12301 tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa
    12361 aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac
    12421 agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt
    12481 ttgatgaaca aattaattat tatggtagaa gtcgattgat ttattttaat gcaatctctg
    12541 tggaattttc gaaagattta tttttttaat acag
//
LOCUS       DASJOX010000071        12441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJOX010000071 DASJOX010000000
VERSION     DASJOX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.072"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            62..247
                     /locus_tag="U4A12_001858"
     CDS             62..247
                     /locus_tag="U4A12_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1774212.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            259..507
                     /locus_tag="U4A12_001859"
     CDS             259..507
                     /locus_tag="U4A12_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774213.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            538..717
                     /locus_tag="U4A12_001860"
     CDS             538..717
                     /locus_tag="U4A12_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774214.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(760..1230)
                     /locus_tag="U4A12_001861"
     CDS             complement(760..1230)
                     /locus_tag="U4A12_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774215.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1317..2015
                     /locus_tag="U4A12_001862"
     CDS             1317..2015
                     /locus_tag="U4A12_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN1774216.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2193..2534
                     /locus_tag="U4A12_001863"
     CDS             2193..2534
                     /locus_tag="U4A12_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774217.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2527..3198
                     /locus_tag="U4A12_001864"
     CDS             2527..3198
                     /locus_tag="U4A12_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1774218.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3204..3890
                     /locus_tag="U4A12_001865"
     CDS             3204..3890
                     /locus_tag="U4A12_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774219.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLSH
                     ETIKKLRIGG"
     gene            3893..4723
                     /locus_tag="U4A12_001866"
     CDS             3893..4723
                     /locus_tag="U4A12_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN1774220.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            4739..5590
                     /locus_tag="U4A12_001867"
     CDS             4739..5590
                     /locus_tag="U4A12_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1774221.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            5587..5946
                     /locus_tag="U4A12_001868"
     CDS             5587..5946
                     /locus_tag="U4A12_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN1774222.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            5961..6122
                     /locus_tag="U4A12_001869"
                     /pseudo
     CDS             5961..6122
                     /locus_tag="U4A12_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            6119..6424
                     /locus_tag="U4A12_001870"
     CDS             6119..6424
                     /locus_tag="U4A12_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774223.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            6424..6735
                     /locus_tag="U4A12_001871"
     CDS             6424..6735
                     /locus_tag="U4A12_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1774224.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            6732..6905
                     /locus_tag="U4A12_001872"
     CDS             6732..6905
                     /locus_tag="U4A12_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774225.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            6902..7318
                     /locus_tag="U4A12_001873"
     CDS             6902..7318
                     /locus_tag="U4A12_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEN1774226.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            7318..7986
                     /locus_tag="U4A12_001874"
     CDS             7318..7986
                     /locus_tag="U4A12_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1774227.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            7983..8360
                     /locus_tag="U4A12_001875"
     CDS             7983..8360
                     /locus_tag="U4A12_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774228.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            8357..8656
                     /locus_tag="U4A12_001876"
     CDS             8357..8656
                     /locus_tag="U4A12_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774229.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            8646..8804
                     /locus_tag="U4A12_001877"
     CDS             8646..8804
                     /locus_tag="U4A12_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774230.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            8898..9314
                     /locus_tag="U4A12_001878"
     CDS             8898..9314
                     /locus_tag="U4A12_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1774231.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9874..10062
                     /locus_tag="U4A12_001879"
     CDS             9874..10062
                     /locus_tag="U4A12_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774232.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            10052..10312
                     /locus_tag="U4A12_001880"
     CDS             10052..10312
                     /locus_tag="U4A12_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774233.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            10353..10580
                     /locus_tag="U4A12_001881"
     CDS             10353..10580
                     /locus_tag="U4A12_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774234.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            10645..11217
                     /locus_tag="U4A12_001882"
     CDS             10645..11217
                     /locus_tag="U4A12_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774235.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            11218..11529
                     /locus_tag="U4A12_001883"
     CDS             11218..11529
                     /locus_tag="U4A12_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1774236.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            11520..11780
                     /locus_tag="U4A12_001884"
     CDS             11520..11780
                     /locus_tag="U4A12_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774237.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            11941..12300
                     /locus_tag="U4A12_001885"
     CDS             11941..12300
                     /locus_tag="U4A12_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774238.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
BASE COUNT         4534 a         1864 c         2739 g         3304 t
ORIGIN      
        1 tcaacgaata ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt
       61 tatgtctgag aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt
      121 caaagaacta gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg
      181 caatcgtgat ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat
      241 ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat
      301 tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca
      361 gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa
      421 tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt
      481 atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg
      541 ggtaaattca acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt
      601 tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg
      661 cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa
      721 tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa
      781 tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt
      841 tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac
      901 ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc
      961 agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt
     1021 agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg
     1081 ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga
     1141 agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa
     1201 cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt
     1261 caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc
     1321 aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa
     1381 gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt
     1441 ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac
     1501 gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga
     1561 aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc
     1621 gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga
     1681 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
     1741 agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa
     1801 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
     1861 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
     1921 catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca
     1981 gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg
     2041 gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt
     2101 gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac
     2161 aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc actctagatc
     2221 aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa
     2281 tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc
     2341 atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg
     2401 aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata
     2461 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
     2521 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta
     2581 aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag
     2641 attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg
     2701 gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat
     2761 gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga aaccgaatta
     2821 tttcctgttt tagattatcg aaaccaacca gttgttaaac caaatgcaat gcaaatcaat
     2881 aactcattga agcgatgctt tgtgaaagca ttggctctac acggactggg attatatgta
     2941 tttcaagggg aagatattcc aacaccacct agaatcgata caaagaaatt aaacatgcta
     3001 gagacgattc tagaagcttt caatgagcag atgggtaaag atatgaccaa aaccttaatc
     3061 gaatatgtta atgagcagac agataaatta gggctcttag ctgataacgt tgaaactatt
     3121 gaacagttaa gctatgagca atgtgccttg atggagcgag caatagcagc taagagaaaa
     3181 gaattagata agaagtgata tgagtgttta aaccattaat cgattcatat tcagcggttc
     3241 tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa tgaggaagtg aacattgatc
     3301 gactaaagac gatgtatgac ggctacgatg gtgatcgagt cattgaaatt cgttttattg
     3361 atcctagacg tttcaccgta cagcaacgaa acttcatcta tgcgctgata ggcgatattt
     3421 ttatcgatac aggcatgcca acggacttct ggaaggaatt cttctacttc cgttttgaag
     3481 gtgtcacagg gcgcaaaata agcctcaaag atgaatcgaa tacgactgtg agtgatgcta
     3541 atgtcttagc aaatatcatc ttagatttca tctttgaaca tcatattcct ttcaaagaag
     3601 gctatgagat tttaccagcg aatcaagaat attacttcta caaatgcatt acaaaaagag
     3661 tttgttgcat ctgtggcaaa acaggagctg acatcgatca ctttgacaaa gcgctaggaa
     3721 gacgaaagcg aaaagaagtt gatcattcag agtacacatt tgcagcactc tgcagaatcc
     3781 atcacacgga gaagcacaaa ataggtgtga tcaattttaa aaataaatat caaatcaaag
     3841 gaatcaagtt aagtcatgaa acgattaaaa agttaaggat aggaggataa atttggctga
     3901 gataagttgg atcaaactta aaactactat gtttgacgat gaaaaaatac ggttaatcca
     3961 agctgttcct gagtcggatg ccatcatcgt tatatggatt cgattactag ttttagcagg
     4021 aaagactaac gacgatggtc tgatatatat ccagaggaac atgccttata ccgaagaaat
     4081 gcttgctaca ttgtttggca aaaacgtaaa tacggttcgc ttagcgctaa ctacattggc
     4141 aaatttcaac atgattgatc taagcagtga tggactaatt gccatcagta attgggaaaa
     4201 acatcaaaat atcgagggta tggataaagt aaggctaaaa aatgctgaaa gaaaccgtaa
     4261 atacagggaa agaaagagac aggaacgtct caaattggaa aatgacgtta gcgtgacgtc
     4321 acgtgacggt acagataaag atatagaaga agataaagat atagataaag aagaaaagaa
     4381 aggtaagtat tctgacgaac acttacgcct tgctaaaaaa atgcaaagga atttaactga
     4441 agattttcca aaagaaatga acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt
     4501 gatggaagaa agagataaag catctataga agcgattgag tatgtgatca attggctacc
     4561 tacaaatgaa ttttggtttg gaaatattag aagtgctaag aaattgagag aaaaatttga
     4621 gaagctcaaa ttcgaaatca aagcagacaa gaagaatcat aaaaagcaaa gtcaaaaact
     4681 acagtacagc aatcctagtg aatatgacga cttgccaatt taaaaggaga tgcatcacat
     4741 ggaaagccta gcaaatgcta tggagaagct aataagaaga gtattagtgc aaagcggaaa
     4801 atgtccagaa tgtagcgaac ctttgtatag ttggcgagct aaaaataagg atggttcaga
     4861 acgttgtaaa ccaacatgta tgagttgtgg ttataaagcg ttacgtgtga aagaggatat
     4921 acagaccgaa cggatatata acgacagctt aaaagcacga gcattgagtt tttttcaaaa
     4981 tggttcggta ttaacagata aaactttgtt taaatgcaaa atggagaatt atcacgtagt
     5041 ggaccaagaa acgaaaattg ctttagaaag agctaaaagc tatgtaaatg atgtcctact
     5101 gaaccatcct gcacatttca ttctatcagg gaaatcagga agcggaaaaa gccacttatc
     5161 aatggcgaca gcttgggaaa tacttgagcg ctcaaattat gacaagaaaa tactttttat
     5221 aagctatcaa gagttattag agcaaataaa gttttcttat aacaatgctg aactgagaaa
     5281 agaaattgaa ggatcgctta tagccgatat caaaacaact gatttggtgg tttttgacga
     5341 tattggagct gaattaggta gcggggtatc aaatagtagg caatttacaa acaacacgtt
     5401 aaacacgctc ttagaagcca gacagaacaa ggcaacgatc atcacaacaa acttatctgg
     5461 tcctgaacta agagaagcct acggtgaaag aattgtttct aggatattta agaattcaga
     5521 aggttatgcg ctgaaattcc aacaaacagc agacaagcgc ataaaaccag tgaaaggtag
     5581 tatcgcatga ataaataccg taataaaaaa actgttcatc gaggtatcaa gtttgattct
     5641 atcgcagaag cagagtatta cgatctagcc ttgtggcaag ctgaagcgaa cggctggaaa
     5701 gtaaaacttc aggaaagatt tgagctgatg ccgaaatttg aactagacgg aaagaagtat
     5761 cgcaagatcg agtatattcc cgacttcaca ttttataaaa acggcaaact agtcaaagtc
     5821 gtagatgtta aagggatgca gacaaaagat ttcaaaatca aagcgaaact cttctgcagt
     5881 caatatcgag taccactgat cttagcgaag aaatatcgga atacgttcaa ggaagaacgg
     5941 ttttaacgag gtgatccatc atgacaacag aagaagtgat tcaaatgcgt attcgaagcc
     6001 ttcagcgtga gattgacgat ctgtaacgga caaaggcagt gatggtcaat gaaacggcga
     6061 ggaaggcaat cgatttgcac atagaaaatt taagaaggaa aatccatcga ttggaggaat
     6121 gagcgtggat aagaaagcag caatgaaacg aatcatcgaa ctgacacatt ctgagaattg
     6181 gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtgggctga
     6241 aaagcctaaa cggaaaacgc cgagaaaaat tgcaatctgg catggtgatc gaattctagt
     6301 aacaggtact gctgaacagt tatctgaaat tactggatta agcaaaaaca ttatctggga
     6361 tagagctagg agcttatgga ttgattcaaa aggacgacag tttaggtatg tggaggagaa
     6421 ataatgaacg aactaatcac aaaagtagag cagtgggcta aagacaaggg actggatcaa
     6481 gcggatccaa aagcacaatt tttgaaagta gctgaggaat tcggagaaat tgcatcggca
     6541 atggcaagaa gtaatgatga gctatttaaa gatagcgtag gagacgttat cgtcacgctg
     6601 attattcttt ccatgcaaaa aggggcaaac atacaagagt gtttagaaat ggcgtataac
     6661 gaaatcaaag gacgcacagg aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg
     6721 gaggacagcg aatgatgatg ccaaaagaaa tctattcaga tgcattaaga ggtgctagaa
     6781 atcaattgaa aatggcaaaa agagcatacg aaattcggcc cacaatcgaa aatgaacgca
     6841 gagtaaaagc aattcgccgt agatgttcaa tttacggcga gttacagaag gaggacagca
     6901 aatgataccg agatttcgag cttgggataa acgaaagaac gtaatgagag atgtagccgt
     6961 cttgcatttt actaaaaacg gcaaaacaaa ctttattgaa tattggataa atcctaccga
     7021 attgaaatca tatcacgtgc gaaacatcga gctcatgcaa tcaacagggc tgaaagataa
     7081 gaatggcatt gagattttcg agtgggatat tgtatcagtc agcgtgcgaa atggattcga
     7141 ttatttagat aataaagttt gtgttgtcaa aaattcaata ggacattcgg gattagtgtg
     7201 tgccactgtt gatgaagatt tagagtatca aatttttaac acagagctgt ttgaagaata
     7261 cacgtatgaa gtcatcggaa atatttggga gaatagcgag ttattggagg aacagtgatg
     7321 aataaacagg aattgattga tgaattagct aaatatgtaa agcgttatga gaacgttatg
     7381 gatgagcatg gccaaggaag gtacggcgct tatgaagtat ctttaaagtt ggtgaaaaga
     7441 ctaaatgaat caaaaattac ggacgaacaa gcttggaata aggtagctga ggcttatcct
     7501 gaatcggcac aaagcttgag aaacacttta gataatgctg tatttggtaa gactggtgaa
     7561 ccgcagaagc acgttatgcc gaagtttgtg gcggagtgga tagaatacgc taaaaagaaa
     7621 ggtgatagtc tagctatttc attcaagccg tggaacctct atggtgttga gtatagcaag
     7681 gctgatagat ggattggaga taatcaagaa acgtttgctc gtgcttggct agacggctac
     7741 gaggtcgaga aagagccgtt gtattatgtc ccgctaccat atgaagtttg ggatgaagag
     7801 gcagcggagc ttaaaacaga gtatttgtat ctacattatg agatcacaag tgatgaaact
     7861 agaatatttc ctacaaaaga acctagaaaa ggttttgtcg caaaattaga tgaacttacg
     7921 attaaatcag cagatgaaaa ctactggccg tttgctgtac cagtggaaga ggtggtagaa
     7981 ggatgaaaaa attgcaccaa ctcatatctg agaaagaaag cgaactccaa aatttagaag
     8041 atagtttggg attggggttt ccaattgttg agcaagttaa aatggttcaa atcagtcatc
     8101 tacaacttga attggaagat ttaagacaaa tagaagatcc gtatcaactc aacgataacc
     8161 agcaaatcgt gttggagtgg ttgaaactaa ctgcgtcaac aggaaagcca atgcaagttg
     8221 tattttggat gatgaataat gcagcatggg ggcatttaga cgaattgagg gatcctttga
     8281 tggaattaac tgacaaacag caattcgaag tcctaacagc attcgctcaa tggggattag
     8341 aacaggagga aaaagaatga aactaaaaga cggattttac gctagtagcc acggtatcgg
     8401 cggtttaatg ctagacatgc cgacaaagaa ccctaaaaca cgtaagaagc caaaagtcaa
     8461 agtcggtgac atggttcgct gtgaagcaga aggattcatc tatccatttc gtggatatgt
     8521 agaacacgtc tataatcact cagcgatcat tcgcattgaa aacacgatgg aatgcgacaa
     8581 gttattagcg aaaagcaaag ataatttagc agtggtgaga ttggtggata tggaggttat
     8641 aaacaatgaa atttaaaatc tttgaagagg acactcgcta taaattagaa aaaaaattaa
     8701 atgaatttgc aaagaataat gaaattcagc atatatcttt aacggcttct aagtcaggtt
     8761 atacaactta ctatgcagct gttgtgagct acgtaattca ataaaaggaa ctcggcaaat
     8821 aaaaaagccg gatcactccg actgatgtaa taaatccgac aagtttatta tatcacataa
     8881 aggagcggtt tgacttgatg caattgttac gagaggtaga tttcaaacag acaagatgta
     8941 atgcgagaga tgtgctgaag aactttcggc gcttggagcg gatggcaggt cgctctttga
     9001 tagatattaa gtcgccgatt attacggata tgccgaaggc accgaagcac ggcaataagg
     9061 cagaagacgc gatcattcag atgatggata tagaagcgga gagagacgcg attttagcgg
     9121 ctttgatggc gcttagtctg attagccgtc agatactcta ctacagcttc tgtgacgtaa
     9181 acaagcactc taattatgaa atagggcaat tgatacgagg atacggagag aagaatgtag
     9241 agaagctgaa atccatcgca ttgatcgaat ttgctgaagc atacaaaaaa ggcgtgttag
     9301 ttcagtatcg ttaattttgt agggtttttg tagggatagt gtagggtttt tgagcggttt
     9361 aacgtgatat tatgatagtg tcgaaagatt agtgataggt ctaagacaaa ataataataa
     9421 aaggaacatc gttttattat tgtttcacaa ttaagcttcg atagacagca gcggaaatat
     9481 taagaataag gatgtgaatt ttaactcctt ctaaattgtt cttattatct atcatcagtt
     9541 gctgtctatt gtcattatgt cactgtggtg gaaaggtgta tcgctcatct aaaatttagg
     9601 tgcaaactgc aaggttcgat tccttgccag tgactttggt aaccgaggca tcggcggttt
     9661 aaaaatatag gggtgcgcaa tttcgtacgc gttttgtgca tcttgtaggt tgctattaca
     9721 taattggttg ggttagattg agttttggga ttcggtacaa atgaatcgtc aaatgactca
     9781 agcacaggat cggaaacgtc cctgcctgtg cattacatat tagatcactc tttgagtggt
     9841 ctttttattt taaaaaagag aggattttga aaaatggaat tgattgtatt tactaataac
     9901 ggacaaacat atcattttta tgaagtggag aattttaagc caacaacaac aggatttagt
     9961 tttatttata ctgggaaggc tacgggagtt actcgttcag ctacgtttaa caatacatca
    10021 acagctggtt atgcgcttgc ggaggtacaa aatgaatcat gagaagttta tcgaaaaatg
    10081 caaggctatt gtacgtgaaa gaattgaaaa tgagattgct gacctaagtg gagcagtgcc
    10141 tgagtttagt attttcatag tctggtcatg caaaacactg caaaatagca aagcattagt
    10201 cagcgctagc ttaaaaggag caccgtattt tgaaattacg ttgaacgggg acaaaggtga
    10261 aatctatgta gatacttatc tcaaaaaatc aaatgaatgt atcaaagtct agcaggtgct
    10321 aggctttttc tttacataaa ggaggctgca taatgagaaa ctactggtat gtatcactaa
    10381 ctaatgaata tcctcgaacc attggtgatt gttcagtgcg tgttgtgcgt tctgtacaaa
    10441 tcaaagggaa gtactctatt gtcgaaatgc taagagaagc tacaccaaac gaagtggata
    10501 aatgcaagct gatatattgc ggtcatggct attggaaaga cgagtatatc caatacaaca
    10561 ttgagaggta catcaaatga atgataacta tgattacatc aagttgttag caagtaatta
    10621 ttagatgttt ttaggaggaa aactatgaaa ttaaaagatt atatcagaga agggtataac
    10681 gttgtaactg cacccaatct agcttacaaa attcaagaag attatcccaa tgctttagtg
    10741 tttactgata gagctttaga tgccattcct atagggaagt tattagtaga tcgtttttat
    10801 agcaataatc cagctgttct tagtgctaag ccacttcaaa acgcttatgc tattgaacgc
    10861 tttacttgcg agtttactgg atatgagacg gtagtaccaa tgacaggtga gacaggaaga
    10921 aaagtaattc aacagatcaa aaagaggatt gctgaattga cgttagatga tggaagtaat
    10981 acgaaccttc tagaaatcaa actaagagat actgactcag taccagaagt ttattataaa
    11041 ggtcaacgaa tggatgagat gccgataggt cttgtcgata ttacttatca ttgggatacg
    11101 gaagaatatg aatgccaagt accaaaagga gctaacgaca tcactattga atattatgat
    11161 tcacataata acaagtactt agatagaaaa atcattgggc acaagagaga tatgtaaatg
    11221 attgatgttt caagtaaaca agcaagagct aggttctatg gatcaactga gtggaggaaa
    11281 cttagaagtc aatgcttaga gcgagatcat tatgagtgcc aatggtgtaa agcagagggt
    11341 agactaacaa ctcaacatga ttcaattctt gaagtggatc atataaagga gctggagtat
    11401 catccggatt tagctctaga tataaacaac ctaagaacct tatgcaaaga ttgccataac
    11461 aaaaggcaca accgtatgaa atatcgtggc caacaaaaga aaagaaagtg ggatgatgaa
    11521 tggtggtaag aaaaacagca aacagagatg atgtcaaacg agcagaagca atcgctatgg
    11581 ttgataaggc tattgataag ttggttctat ttaagagtca gctcattgaa ggaacaacaa
    11641 ctgtagagga tatatctatc aacaatagac taataggtaa cgctaatgaa gtaacaggga
    11701 catttaatat taatgtagac attgattata ccttgagtcc atttgcacat attgtagaaa
    11761 gcaacgagca agacggctga taaatcagta taaagcgaag atatatagag tgaaataccc
    11821 ccggtcaaaa gattttgaga agaaataaaa tttttgggaa ccggtggatg gggtcaactc
    11881 tgcaaataaa tgatgctatt ttcacacgac cccctcccca cgtagaaagg aagtgagttg
    11941 atggctgatc tgcaagagag aaataggctt gtttctgctg aagaaaggcg tctaaataaa
    12001 ttgtttaagg atattgataa ggataaaaag aaggtcgttt ctggtttgat tacgcaagcg
    12061 gcacgcttaa aaattcttct tgatgaaatg tggattgata tttccgaaaa aggtgattat
    12121 gagcttttct ctcagtcaga aaatcaaaaa ccttacgagc gggaacgacc agttgctaaa
    12181 cagtataatt cgagagacca agcatatcaa cgtgtaatta agcaacttac tgactatttg
    12241 ccagaagaaa agcgcgaacc tacaaaaaat gcagcgctag atggtagtga tttgctgtga
    12301 caacattaaa gccattctat tttgatgaat acgtagattt atacgaaaaa ggggtaatcc
    12361 cttttaataa agaaagaatt cagttaatta cttatttaaa aaacgaagtg ctagttcggg
    12421 atgatatcta ttttgatgaa g
//
LOCUS       DASJOX010000072        12211 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJOX010000072 DASJOX010000000
VERSION     DASJOX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.073"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            250..525
                     /locus_tag="U4A12_001886"
     CDS             250..525
                     /locus_tag="U4A12_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774239.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            538..2610
                     /locus_tag="U4A12_001887"
     CDS             538..2610
                     /locus_tag="U4A12_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN1774240.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2775..4661
                     /locus_tag="U4A12_001888"
     CDS             2775..4661
                     /locus_tag="U4A12_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1774241.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4673..5620
                     /locus_tag="U4A12_001889"
     CDS             4673..5620
                     /locus_tag="U4A12_001889"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1774242.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5640..6167
                     /locus_tag="U4A12_001890"
     CDS             5640..6167
                     /locus_tag="U4A12_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1774243.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6230..6883)
                     /locus_tag="U4A12_001891"
     CDS             complement(6230..6883)
                     /locus_tag="U4A12_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN1774244.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7015..7857
                     /locus_tag="U4A12_001892"
     CDS             7015..7857
                     /locus_tag="U4A12_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1774245.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8015..8911
                     /locus_tag="U4A12_001893"
     CDS             8015..8911
                     /locus_tag="U4A12_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1774246.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8914..9627
                     /locus_tag="U4A12_001894"
     CDS             8914..9627
                     /locus_tag="U4A12_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN1774247.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9647..10165
                     /gene="msrA"
                     /locus_tag="U4A12_001895"
     CDS             9647..10165
                     /gene="msrA"
                     /locus_tag="U4A12_001895"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1774248.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10162..10389
                     /locus_tag="U4A12_001896"
     CDS             10162..10389
                     /locus_tag="U4A12_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN1774249.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10541..11092
                     /gene="lepB"
                     /locus_tag="U4A12_001897"
     CDS             10541..11092
                     /gene="lepB"
                     /locus_tag="U4A12_001897"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1774250.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11175..11675)
                     /locus_tag="U4A12_001898"
     CDS             complement(11175..11675)
                     /locus_tag="U4A12_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1774251.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11954..12208)
                     /locus_tag="U4A12_001899"
     CDS             complement(11954..12208)
                     /locus_tag="U4A12_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1774252.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4248 a         2077 c         2463 g         3423 t
ORIGIN      
        1 cgcagcccca tttttttgtt gtgataaaag aacaagaaag gcaggaatgg gaaagtaaac
       61 atccttttgt cagaaacttt tatctttctt aagttttact tgatatctga tactatttgg
      121 aatagaatga ggcgttgaaa aatttatgga aatttctaac tagcaataaa cgatgaaaca
      181 atgaaatatt tttcatccaa aaagaacttt gctataatga tggaagtttg tgtataggag
      241 atctggaaaa tgagagagag ttttgaacaa caaaaaaaac tgttgcatga tcgctatggc
      301 gccctttcga tggatgaccg ccgtcaaata ctttgtaagt tgcgtaaaag aaatatattg
      361 atgtatcgtc agttggaacg tctgaaacat gatctgcttc gtttggagtc aaaacgtgta
      421 cagtgtgaat tagaggggaa ccaaacacag gttgaagtag tcgaaaccaa aattttaaag
      481 aaaaaagaac aatttttaaa aatgcttaca caaaataaaa aataaagggg atggacgatg
      541 gaatttgctg agttagttat tgtatttgca gtaacgatca ccttatcgaa catagctagc
      601 cgcattttgc caatgattcc agctccactg atccaaattt tccttggagt ggtattaggt
      661 ctgacagagt ggggacaatc aatcgatttt gaacctgaat tgttcttagt tatgattatt
      721 gcaccattgt tatttagaga aggagaaaaa gcagatattt cttcgattct caaaaatttc
      781 gggacaattt tgttcttggc atttggagga gtcattttga cccttgtcgg ggtaggtgcg
      841 acgttatcat tcctgttgcc aagtgtccca cttgctgcct gctttgcttt tggagcagcg
      901 ttaggtccga cagatgcggt agcagtcagt tctctttctg gacgagtgaa tatccctaaa
      961 aaggcgatgc atattttgga aggagaaggt cttttgaatg atgcatctgg tgttactgcc
     1021 ttccagtttg ctcttggtgc gctgatcacc ggatcttttt cagcagtaaa cgcggggatg
     1081 agtctagtag tttctagtat tgggggagct ttgatcggct ttttgcttgt ctggttcaaa
     1141 caaaaaataa ttcatttgat cgaaaaagca tcagcacaag atgtcacagg gtatctgctg
     1201 atcgaacttt tgcttccttt tctagcctat gtattggcag aggcatttga tgtatctgga
     1261 atcattgctg cagtggcagc aggtattctc caagcttctg gttttaggaa aatctctgtg
     1321 tttgatgcag aattgtcgag cttgtcccac agtacatgga caactattgc ttttacactg
     1381 aatgcattag tatttatatt tttaggaatc gaattgacgc aagtgttctc gccagtatgg
     1441 ggagacggtt tatatccaaa cggtctttta ttagcgatta tcgtattaat ctctgtcatg
     1501 ctttttgtga tcagattcat ttctatcagt cttttttatg tattcaaaga tggcagtaaa
     1561 aagttcaaaa aacagttgaa cgagatcttg atcttaacat ttggcggtgt aaagggaaca
     1621 gtcagtttgg ctacgatttt tattttgcca ttttcaatca ataatatgat gttttaccag
     1681 cggtctttgc ttttgtttct gacggcgggt gtcatcttag tgactttggt catagggatc
     1741 attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa gcacggattt gaatgctttg
     1801 atgattttag aagaggtcgt tgaagttttg cgaaaagaaa taaaggaaat tgatcgtaat
     1861 acaaaagaat ttttagccac tgaagcagtg atagaaaatt atcaagaacg gatacgtgat
     1921 ctgtacttgg aagatttaac cgatgatgaa aagcaagaag tacaagaaat acaagcactg
     1981 attttgtcga tcgaacggga tggattggat gaaagttatc gttcgggcaa gctttcttct
     2041 aatggttatc gtttttactc ccgttttctt tctcgattcg aacattctat cacaagccag
     2101 attttgtctt ttatcggctt ttggttcatt gttgtccgta ggttaatgcg aatcgttctc
     2161 catccgaaaa tgttttggca aagaagatat gctgatcgtc agacatttat tagtgatgaa
     2221 gatatccaag aaatcagaga gacatatcaa aaaaatactc agttgattat tgaaagttta
     2281 gataacttga ctgatattta tgatgaaacg atggttcaat tttttatcca gcaacgcaaa
     2341 cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt cgtggatgat ccagcaagat
     2401 agtctgttta cgaaaaaaat gctaagaggt tattatcttg aaagaaaaat cattgatgaa
     2461 tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca gctatcgcag gaatatcaat
     2521 ttgttagaat cttatacgat gaacaaacca tctgataact tttctttccg atttgcgttt
     2581 cgagcaaagt caaaaaaacg agtccaataa aataaaaagg taaaaatttt caagttttat
     2641 aaaaaacaaa acatatcaga acaggatcga atcggtcctg ttcttttttt gagaacacaa
     2701 aaagaacaaa taccaaaaaa attttcctga agttcactat ttttatgata caatatccaa
     2761 gataaggagg gaacatgttg aaagaattaa aagtaactga cctcaaaaaa acttatggtg
     2821 aaaaagattt gtttgaccag atttcttttt tggtccacga aaaagaccgt atcgggttga
     2881 ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat tattgccggt atcgatagca
     2941 gcgacggtga tcggcaaaca gtgttttatc caactgatta ccgtattggt tatctttctc
     3001 aagcagctga attttccgat gaattaactg ttctccaagc agtctttcaa ggaaacagcc
     3061 ccttgatcca aactgttcga gcatatgagg aagcattgat cgaactaggg gaaaacggag
     3121 aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga acaaatgaat aaagaagatg
     3181 cttggactac agataccaac gcaaagatta ttttgcaaaa attaggaatc agtgaattag
     3241 ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt
     3301 tgattgatga acccgattta ctattactag acgaaccaac aaaccattta gattatgaag
     3361 cgatcgagtg gttggaaaac tatttgaaac aataccgtgg cgcactattg atggttaccc
     3421 acgatcgtta ttttctagat agagtagcaa accgaatctt cgaactttca tttggaaaat
     3481 tatacgagta taaaggaaat tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg
     3541 tagaagtaga acaagaagaa aagcgcaagc ggctttataa acaggaatta gcttggatga
     3601 gggctggcgt acaggcacgt ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc
     3661 taaaggagaa cttgtaccaa gtaaaccaag aagatgattt agagctgaac ctagcgacac
     3721 aacgtttagg gaaaaaagta ctggaaatca aagatggcag ttaccgtata aatgaccaaa
     3781 cactgctaga acatttagat ttattgattc aatctcggga aagactaggt attactggta
     3841 aaaatggtgc gggaaaatcg acattgctta atattctagc tggcagaatt cccctggata
     3901 gcggtacaat gtctatcggt gaaacggttc atcttgcgta ttatacacaa gaaaatgaag
     3961 aaatggcacc agataaacgg atgattgctt acttacaaga agctgctgaa gaagcaaaaa
     4021 cagcagatgg cagtcaaatc ggagtagcag aattacttga acgattttta tttccaagat
     4081 ttatgcatgg gacactgata ggcaaacttt caggcggaga aaaacgccgg ttatttttgt
     4141 tgaaactgct gattcagcaa cctaatgttt tattattgga tgaaccgacc aatgatttgg
     4201 acatagcaac attgacgatt ttggaagatt attttagaag ttttccaggt gctgtgatca
     4261 ctgtttccca tgaccgttat ttcttggaca aggtagcaga taagttatta gtattccaag
     4321 gaaacggaaa acaagaactt tattacggaa acatgtccag ttatttactg aagcaaaaag
     4381 agacccagca gcctgctgaa aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa
     4441 agaagaaatt atcttacatg gaacaaaaag aatgggaaac gattgaagat gagattgcag
     4501 aacttgaaga aaaaatctca ttgctccaag aagagatgaa tcatcaagga gataatttca
     4561 ctcgattaca agaattgcaa aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa
     4621 tggcacggtg ggaatacttg agtgaatggg tagaagatta ggaggaaaaa ggatggaaca
     4681 agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg catcgtaaag aagacagaac
     4741 aggcacaggc acattaagct tattcggata ccaaatgcgg tttgatctgc aaaaagggtt
     4801 tcctttgtta acaacgaaac gagtaccttt tggattgatc aaaagcgaat tgctttggtt
     4861 tttaaaagga gatacgaata tccgctatct attacaaaat aaaaaccata tttgggatga
     4921 atgggcattt gaacgctaca tcaaaagtcc agactataat ggaccagata tgactgattt
     4981 tggccataga tgtttgacag atcctgaatt caatgaagaa tataaaaagc agagcaagct
     5041 tttttgcgag aagatcctaa ccgatgattc ctttgctgaa acatacggcg atcttggaca
     5101 catctacggc tatcaatggc gtcactggga aacaaaagac ggtggcttta tcgatcaaat
     5161 caaacaagtg atcgaagcaa tcaaaaagac tcctgattct agaagattga tcgtttctgc
     5221 atggaatccg gaagatgttc ctagcatggc tttgcctcct tgtcatacga tgtttcaatt
     5281 ttatgttcaa gagggacggc taagctgcca attgtatcaa cgcagtgggg atgtgtttct
     5341 aggcgttccg ttcaacattg caagttatgc gttgctgact cacctaattg cacatgaaac
     5401 aggtttagcg gttggcgaat ttgttcacac attaggagat gctcatcttt atatcaatca
     5461 tatcgaacaa atgaaagaac aattgagccg agaaatacgc gcatttccga ctttagtttt
     5521 gaatcaggaa aagcagtcag tatttgattt tgagatggaa gatatccaat tagaaggata
     5581 tgacccacat ccggcgatca aagctcctat cgctgtttag aatcagaaag gatgagaaaa
     5641 tgttagctgc aatttgggca caggatgaaa acggattgat tggaaaagaa gaccgtcttc
     5701 cttggcgttt gccaaatgat ctgaaattct tcaaacaaat gacagaagca aatacactag
     5761 tgatgggacg aaaaacattc gaagggatgg gtagccgtcc gttgccgaat cgtcagacga
     5821 ttgttttgac gagagattct tcctataaag cagaaggcgt gcatgtcatg catagtgtgg
     5881 aagacgtgct tgctctggaa aaagaaacag acggtatctt tttcatcgct ggagggtcag
     5941 ctgtttatca agaatttctg ccattttgta cgatattata ccgtacagtc atccatcatg
     6001 tattcgatgg agatgcttat tttccacctg ttgactggtc tgattggtca ctgatcaata
     6061 ctagtcaagg agaaatggat gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc
     6121 gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac gatataaaaa agctatggat
     6181 agactagctg ttatttagag ctgtcccatc catagcttta ttttttttat taagtaaaga
     6241 ataaaacaga gaagtacata aaacctgcac ctaacataac aaacaaatgc caaacaacat
     6301 gcatatagcg gatagaattc aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc
     6361 ctgctaccat caaaataaca ccagtcattc caagagagtg ataaagagga acagcagcca
     6421 cgacacagag ccaccccata aaaatataga tgatcgtaga tacttttgat actctctccc
     6481 gtttatgcaa cgtcaacgct ttatatataa tgccaagaat cgcaaacaac cagattaata
     6541 caaataaagt ccaacctagc catcctcgga tactcagcaa acaaaaagga gtataacttc
     6601 ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg gaacaccttt tttgcttttg
     6661 taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa aagaatcatc atcgatccat
     6721 agatagcata agagacaata tggagggagg aatgcaagtg ggcacctttt actaaaagaa
     6781 ccactagtcc cgcgatactc aatccagtcc caattccatg ggtgactgca ttaaacactt
     6841 catttaatat ttcatacgtt cgattaaatt tcgttttttc catgaaaacg gctcctttac
     6901 taggtgtaat taatgaaaaa tcattatttg tctgttatac taaaaataga aattgtctta
     6961 catgtagttt aacatgaata aggcatcttg ttaaagaaag agagagagaa aataatgaca
     7021 aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta
     7081 ggcattcgtg tcatgccgct atccatcatg atcgacggaa cagtatatcc cgatgatgac
     7141 caactagacg gcgaaagatt tatggaaatg atggcgaaag caaaaaatct gcccaaaaca
     7201 agccaaccac caattggaga attcatctcg atgtatgatg agctaggaga agacggcagt
     7261 gaagtgattt cgatccatat gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa
     7321 gccagtaatc taacaaaaac gaaagtaacg gttatcgaca gtgataccac tgaccaagga
     7381 ttatcttttc aggtaattcg agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa
     7441 atccttgaag aagtcaaaaa agtacaagac aatacaaaat tatacatcgg aatatccaca
     7501 ctagataact tggtaaaagg tggaagaatc agccgaacaa ctggtttgct gtctagtgtt
     7561 ttgaacatac gcgtggtgat gaatttcgcc cattctgaat taatccctgt aactaagggg
     7621 cgaggaaaga aaacgttcga taaatggttt aataatttga aaaatgaact gaaagaattg
     7681 ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa aagaattggc gcaagaattc
     7741 aaagagggtt tgcaagaatt attcccggac atgcatatcc ctgtactgca tacgaatccg
     7801 gttatcgcta cgcatactgg taaagacgcg tttgcaatca tgtattatgt agattagtcg
     7861 aaaagaggat aagccctcag cctgaaaggc ttgcagctta ttctcttttt ctttataaaa
     7921 ttaggaaaat aattgttttc ttttttcata aagactacct ttttgataaa atttttgata
     7981 acattagaac caatcgaata caagaggtga aaaaatgaaa ttaacgaaac gacatttgat
     8041 cctcgttttg ttcccattat taattgcgat catcatctat gcaggtttag ctgcttctat
     8101 accgaaagct ggcccactaa tgaaaccagt aactactgtg agagaagatc caaataaaaa
     8161 agaaaaactt cactacatag ctatcggaga ttcgctgact gaaggagtag gagatcaaac
     8221 caagcaagga ggattcgttc cccttgtcgc aaatgatcta aaagaccgtt ataatttgac
     8281 cgcggttgaa acagaaaact atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg
     8341 agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca gctgatttta tcacactgac
     8401 tgttggcggt aatgatttga tgaaagttat ccaaaataat ttattcgggt taacggtcaa
     8461 ttcctttaaa aagccactga aaaactatca ggaaaatgtc acagaactat tggaagaatt
     8521 tcgatccctc aataaaaccg cacctattta cattttaggc atctacaatc cattttattt
     8581 gaactttcct gagatcactg acatgcagac aattgttaac aattggaaca aaggaacaga
     8641 aacggtgaca aaaaaaatgc cgaactgcta ctttatccca atcaatgatt tgctttatca
     8701 aggattgaat caggaagtag gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac
     8761 agaaaatcta aatggcatca aaaataatgt gctctatgaa gaagatcatt tccatccaaa
     8821 taatttaggt tatcaattga tggcaaatgc agtgagagat cagctgatcg agacacaaaa
     8881 ccaatggtta gagaaggagg atgatcaata aacatgaaaa caagatctga cgtatcttca
     8941 aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc ttatccttct tggggtagta
     9001 cttggttcga ccgtattttt aggcagccgt atttttgcta atcgggaacc agaactccca
     9061 gaaataccag ctcttacgga aagacaagga gatcctgtct taacgatcaa ttcaaataaa
     9121 gaaaaagtca atcaaatcat cagctttttc cttagtgagt atcaaaaaga tagtgatatt
     9181 gaatataaat tttatttgga aaatgaagca ctactcaatg gtactttcga agtactaggt
     9241 tttcctatcg atttctattt atattttgat ccgtatgtga tggaaaacgg aaatgtccaa
     9301 ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat
     9361 atgatcaaga gaaactataa attaccagat tggatcgaag tcaatacaga tgatctgact
     9421 atcatgctta ggttggatca attccgaatg caaaacggga tgtatattaa agcagataaa
     9481 atcgacttag taaatgatga cattcggttt agtctatatt tgccaaaaga tacctctgat
     9541 gcttcaaaca actcaaatac caacaaaact agcaccagca gtactgaaag caagacaaaa
     9601 gaaaataaaa aatcatcgga tgagtagtat tagaggggga atcataatgg aacgagcaat
     9661 atttgcagga ggctgcttct ggtgtatgat ccagcctttc gacacgcttc ctggtatcca
     9721 tacagtgatg tcaggatata ctggagggca tgtaccagat ccgacttatg aacaagtaaa
     9781 aagccaaacg actggacata cagaagcagt ggagattatc tatgatccaa cattgatttc
     9841 atatgaacaa ttagtagaac tttactggca gcaaactgat ccaactgacg ctttcggaca
     9901 attcgaggat cgcggtgaca attatcgccc ggtgatcttt tatttgacag aagaacagcg
     9961 gatcattgct gaaaaaagta agaaacagtt agctgaaagt ggacgtttcg aacagccaat
    10021 cgttacaaaa atcgaaccag cccaaccatt ttatctagca gaagaagacc atcaagagta
    10081 ttataaaaag catcctgaaa attttaaaag aaaccatgca cgccgggctg cctttatcga
    10141 agaaaattgg aggaacgaag ggtgaatcga agcttttttc attatttaat gacactgcga
    10201 ggcggtggca cacatgatgc tttatctgtt ttcgctacag aagccggtaa agacatccaa
    10261 tttcctaagc acagctctag ctacgaagaa atttctaatt atctggaact gaatgtcgac
    10321 tatttgccgt ccatggatat ctttgacgaa gcatgggaaa aatatttaga aaataatcat
    10381 gacatataag agatagtttt gtactttatg atcaaaaaag ttttttgtcc aatagcctaa
    10441 ttgaaagaat tatttttatt tcagctatct agtcgcattt ttttgatatt tttcttataa
    10501 taaagaaaga atgaaaaaaa gagggatgga ttgatagaga ttgacaaaaa aacaacgcta
    10561 tattaatcgc ttctggcttg tattcaaata cttacttgtt tctgtattcc ttgcgttcat
    10621 gttgcgggga tttttattca tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt
    10681 gaagcaaggt gatatggttg tgatggaaaa attttcggag atcagacgat ttgatattgt
    10741 tgtttttcaa ttagcagatg gcacgattta tatcaaacga gtaatcggtc ttccaggaga
    10801 aaatgtcagc taccaaaatg atcaattgaa gatcaatggg aaagtagtta aggaacctta
    10861 tctaactaaa aacctgaaat cagatcatgc aaatgcatcc tatacgacgg actttacact
    10921 acaagagcta acgggacaat ccaagcttcc ggaagataat tattttgtac ttggagataa
    10981 tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc aataaaactg atattctagg
    11041 taaagcaaga tttgtctatt atccacttga tgaaataaaa tggatccagt gagaaacaat
    11101 aaagcgtgca gtctgttgag actgcacgct ttattgttta atccaaaatc agtttactac
    11161 cttagtacgt aaaatcaaac agctcgttta aacacttttc tagcctctaa agttttgata
    11221 aatgaataaa caaccattat ttgaataggg atttggatca tattcctgat gaatctacct
    11281 agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca tcagccaaga attgataaaa
    11341 aggctgactg ggatcataat caataattgg gcagcgatga cggaaggcag gtttatcttt
    11401 ttatgatata aaaataagcc attaataaat ccaccaagga aagccgtgat aagaaaagca
    11461 gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag tgttcgacaa catttcaagc
    11521 aaaccagccc agatcggtcc atatagataa cccataagcg ccatacttaa aaatccgaat
    11581 gtgatttgaa aataagcagg accaatcgct atcttgtata agatgacatc aagggctaat
    11641 aaaagagcag aggtcgacaa caatcttgca ttcatttcgg gcaatctaaa aacgtctttt
    11701 gcaatcaaag tgttcaaaat attctctcct ttgtcagtta ataataaaag gataccataa
    11761 aatcaccaat gtgtcatgac acattggtga tttacataca ctggcatcta acaattaaaa
    11821 aaagggtgag aagactgctt ttataaactg taaaaaagag attgtacgaa taaaaaaaat
    11881 agaggcggag aatacttatc gtatcatccg cctttgtttt tttgttataa aaaacgtcag
    11941 attagttatc tgcttacatc ttcatccata cgggttctaa tgtatttccg tctagatctt
    12001 ggacttctaa tgaaaacatt tcttcttcag gcattcccat atctacttcg tggaaagtac
    12061 caccattttc ttttgcaatt tgagcaaatt tttttacttc atccgcgttt tcaacactaa
    12121 acgaaatcaa agcaccactt gtttttttat tatcagctat ttctttatct tttataaatt
    12181 ttttataaaa atcatgagag agaagcatta t
//
LOCUS       DASJOX010000073        12194 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJOX010000073 DASJOX010000000
VERSION     DASJOX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.074"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="U4A12_001900"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="U4A12_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN1774253.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="U4A12_001901"
     CDS             complement(642..1364)
                     /locus_tag="U4A12_001901"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN1774254.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="U4A12_001902"
     CDS             complement(1500..2381)
                     /locus_tag="U4A12_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN1774255.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="U4A12_001903"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="U4A12_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN1774256.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="U4A12_001904"
     CDS             3621..4100
                     /locus_tag="U4A12_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN1774257.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="U4A12_001905"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="U4A12_001905"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN1774258.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="U4A12_001906"
     CDS             5401..5529
                     /locus_tag="U4A12_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1774259.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="U4A12_001907"
     CDS             5533..6078
                     /locus_tag="U4A12_001907"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1774260.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /locus_tag="U4A12_001908"
     CDS             complement(6532..8223)
                     /locus_tag="U4A12_001908"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN1774261.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="U4A12_001909"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="U4A12_001909"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN1774262.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="U4A12_001910"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="U4A12_001910"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN1774263.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="U4A12_001911"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="U4A12_001911"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN1774264.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3340 a         2527 c         2203 g         4124 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taat
//
LOCUS       DASJOX010000074        12171 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJOX010000074 DASJOX010000000
VERSION     DASJOX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.075"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..306)
                     /locus_tag="U4A12_001912"
     CDS             complement(133..306)
                     /locus_tag="U4A12_001912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774265.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            592..1920
                     /locus_tag="U4A12_001913"
     CDS             592..1920
                     /locus_tag="U4A12_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN1774266.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="U4A12_001914"
     CDS             2122..2955
                     /locus_tag="U4A12_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN1774267.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="U4A12_001915"
     CDS             2971..3708
                     /locus_tag="U4A12_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN1774268.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="U4A12_001916"
     CDS             complement(3863..4831)
                     /locus_tag="U4A12_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN1774269.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="U4A12_001917"
     CDS             5063..5896
                     /locus_tag="U4A12_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN1774270.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="U4A12_001918"
     CDS             5930..6769
                     /locus_tag="U4A12_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1774271.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="U4A12_001919"
     CDS             6795..7355
                     /locus_tag="U4A12_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1774272.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="U4A12_001920"
     CDS             7554..9275
                     /locus_tag="U4A12_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN1774273.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="U4A12_001921"
     CDS             9440..10582
                     /locus_tag="U4A12_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1774274.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="U4A12_001922"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="U4A12_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1774275.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DASJOX010000075        12093 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJOX010000075 DASJOX010000000
VERSION     DASJOX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.076"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            474..725
                     /locus_tag="U4A12_001923"
     CDS             474..725
                     /locus_tag="U4A12_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774276.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="U4A12_001924"
     CDS             759..1037
                     /locus_tag="U4A12_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774277.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="U4A12_001925"
     CDS             1634..1891
                     /locus_tag="U4A12_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774278.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="U4A12_001926"
     CDS             2004..2615
                     /locus_tag="U4A12_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774279.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="U4A12_001927"
     CDS             2794..3351
                     /locus_tag="U4A12_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774280.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="U4A12_001928"
     CDS             complement(4050..4571)
                     /locus_tag="U4A12_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1774281.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="U4A12_001929"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="U4A12_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN1774282.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="U4A12_001930"
     CDS             8651..9538
                     /locus_tag="U4A12_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN1774283.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="U4A12_001931"
     CDS             9535..10704
                     /locus_tag="U4A12_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774284.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="U4A12_001932"
     CDS             10728..12083
                     /locus_tag="U4A12_001932"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN1774285.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         4023 a         2030 c         2657 g         3383 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat ttt
//
LOCUS       DASJOX010000076        11689 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJOX010000076 DASJOX010000000
VERSION     DASJOX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.077"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4A12_001933"
     CDS             6..305
                     /locus_tag="U4A12_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774286.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4A12_001934"
     CDS             378..1739
                     /locus_tag="U4A12_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1774287.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4A12_001935"
     CDS             1761..2021
                     /locus_tag="U4A12_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774288.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4A12_001936"
     CDS             2035..2787
                     /locus_tag="U4A12_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN1774289.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4A12_001937"
     CDS             complement(2881..3486)
                     /locus_tag="U4A12_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1774290.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4A12_001938"
     CDS             complement(3539..4513)
                     /locus_tag="U4A12_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN1774291.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4A12_001939"
     CDS             complement(4891..5610)
                     /locus_tag="U4A12_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1774292.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4A12_001940"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4A12_001940"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1774293.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4A12_001941"
     CDS             7161..8558
                     /locus_tag="U4A12_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1774294.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4A12_001942"
     CDS             8639..9079
                     /locus_tag="U4A12_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1774295.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U4A12_001943"
     CDS             9227..9553
                     /locus_tag="U4A12_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1774296.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U4A12_001944"
     CDS             9554..10303
                     /locus_tag="U4A12_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774297.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U4A12_001945"
     CDS             complement(10383..10838)
                     /locus_tag="U4A12_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1774298.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U4A12_001946"
     CDS             complement(11265..11540)
                     /locus_tag="U4A12_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774299.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3701 a         2022 c         2368 g         3598 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctga
//
LOCUS       DASJOX010000077        11667 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJOX010000077 DASJOX010000000
VERSION     DASJOX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.078"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..215)
                     /locus_tag="U4A12_001947"
     CDS             complement(36..215)
                     /locus_tag="U4A12_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774300.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(596..1303)
                     /locus_tag="U4A12_001948"
     CDS             complement(596..1303)
                     /locus_tag="U4A12_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774301.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1296..1733)
                     /locus_tag="U4A12_001949"
     CDS             complement(1296..1733)
                     /locus_tag="U4A12_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774302.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1748..1999)
                     /locus_tag="U4A12_001950"
     CDS             complement(1748..1999)
                     /locus_tag="U4A12_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774303.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(1999..2205)
                     /locus_tag="U4A12_001951"
     CDS             complement(1999..2205)
                     /locus_tag="U4A12_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1774304.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2642..2902)
                     /locus_tag="U4A12_001952"
     CDS             complement(2642..2902)
                     /locus_tag="U4A12_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774305.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2977..3180
                     /locus_tag="U4A12_001953"
     CDS             2977..3180
                     /locus_tag="U4A12_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEN1774306.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3321..3806)
                     /gene="ssb"
                     /locus_tag="U4A12_001954"
     CDS             complement(3321..3806)
                     /gene="ssb"
                     /locus_tag="U4A12_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1774307.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3809..4708)
                     /locus_tag="U4A12_001955"
     CDS             complement(3809..4708)
                     /locus_tag="U4A12_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1774308.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4721..5314)
                     /locus_tag="U4A12_001956"
     CDS             complement(4721..5314)
                     /locus_tag="U4A12_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1774309.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5390..5539)
                     /locus_tag="U4A12_001957"
     CDS             complement(5390..5539)
                     /locus_tag="U4A12_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774310.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5555..7759)
                     /locus_tag="U4A12_001958"
     CDS             complement(5555..7759)
                     /locus_tag="U4A12_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEN1774311.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7770..9230)
                     /locus_tag="U4A12_001959"
     CDS             complement(7770..9230)
                     /locus_tag="U4A12_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1774312.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9291..11663)
                     /locus_tag="U4A12_001960"
     CDS             complement(9291..11663)
                     /locus_tag="U4A12_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774313.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1957 g         4385 t
ORIGIN      
        1 gctatcactt ttagttttta ggataaaaag tgacgtcact ttttgagtgc tggtacctca
       61 aatcctgctt tttcttgggc aatcaataaa tctaacgcaa tacacataat cctggattca
      121 cttttgccgt acttctgctt tagttgctgc aattcttttt gtctcttttc atccaacgtc
      181 acgggtaacc gtttatttcc tattgcaatt ggcattagta ctctctcctt tcatcgaaat
      241 aaccctatta aaaatatggc tgcaatcaac ttgaatatta gactcacaag ggttaacaca
      301 aagcgccaaa agatcaaaca aaataccagt attcctaaga atactacgaa gtttaaatgt
      361 agagctgcac catgacgcat aagtatctcg ccaacaaaca gtacgaacaa tagaggaatg
      421 attttattca tatggatctc cttctttctc ttaggttatc gggctttgcc cgcttatttc
      481 gcaccaccta agcggcgggg gcggttggat cgattggtgc gaaataagaa ccattccctt
      541 aatttcaagg ttttttgttc ttatttcaca catcctcact tttcggtata aatggttatt
      601 gacctttttt cctttcaaat cgttgatatg gcattcgttt acggtcaagt tcattttgga
      661 tttcttctgt atcgtcgtaa gtgtaggtgt ctaaaaaatc agttaaagtc gtgtaccagt
      721 cgtcgcctgg gttatctact tcttctacat attccctcaa aaatgcttcg ggtacagtga
      781 gttcataatc cattcccatt aacacgatag attccgtccc acacgtcaaa aatctagtaa
      841 atacaaggta ataatcgtct accgtcgcat ctttgctgtt tagctcttcc atttcttctt
      901 ccattaattc catgctctca aaaattgaat aatattctga ttctgagtaa tagtgtttcc
      961 tgcaaatcgt gtagctgaca aagttttcga ccagctcact cctttctagg taaacattca
     1021 tgatatattt ttctgtttct agtcgtagaa gctccgctcc tttttttaat gtgtcaggac
     1081 gacgtttata cacgtctaaa cgattatggc tgactgtaac cttcgtttga ctcgtttcac
     1141 tttccatata caactgttct tccactcttt gaataaagta cttcatttct tccatacaac
     1201 taaagtcttt ttgaatccgt ctgattactt cattcaagtg ttctttgttt gttaaaaatt
     1261 gcgaaacatc ttctgttgtt agctgataaa ccaccttatt cattatcttt ctcctccgat
     1321 tcaatcgtat aaaatgcacc atttttttcg atattttcaa aagtatctag ccagtcgcta
     1381 gccattgctt tagctttctt aatctgtgcg aattttgcat aagtcagttc taaggcttga
     1441 tattcggaag gtttgatagt gcttggtttg acacctactt ccaagcatag taaggcttgg
     1501 aaaaaccatt ttccattttg ttttttcagt tcttccagtg ttcgatagta cgtcgcaatg
     1561 agattcattt cgacacctga ttcaatcatt tcttccactg cgactgacaa tttttccttg
     1621 taatgttttt tgggtacacg tgtttcatgg tataggaacg tcatatcctc cattttttgt
     1681 gccattttat ccaaaggtaa tttgacaaat tcgtcatagt aattttgagc catatttttt
     1741 ctccttttta gttctctaat ttggttaacc aatattcgat ttcataagtt tccatgtttt
     1801 cttgataatg cagttcatat cctgcaatct tcactaggtc ttcaaatagc atagtcggtg
     1861 catcctctac taaaggggtt tctatcgtcc agtaccgcgg aataagtaaa gcttctgcta
     1921 ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt acagtcaata
     1981 atttgtgttg tgttttcatc accgtttctt tactttctca tcaatcaaaa cataatcgcc
     2041 ataagacaag gtatattttt tcccgcatgt tggacattga tacaaacgat agcttttgat
     2101 acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat agtttgggaa
     2161 taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg attctgcctc
     2221 ctcttaggaa aggttttatt tcctttcgtt agttctttct ttattgattt gttggccaga
     2281 atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac ccttgtcctt
     2341 gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg gcaaatcaat aaagagatag
     2401 cgaacaggaa cgaacctttt ggtgagagga agttttttct attttgtctt gctttgcaag
     2461 acaccatatc ttagtgaacg tgcaacgttc acttcctttc ttctcgaacg gcggtggcgg
     2521 ttggagtgag agaaaggttt ttcaattgag aaggcacttg tgtcttccta aaaaggtctt
     2581 ttcctgtttc tatgtaaaac aaaaagagct gcaatcctca tttaggactg cagctcttct
     2641 tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc gattcaatga
     2701 ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt ccatcatcca
     2761 taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc gattcatgaa
     2821 ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg attttatcaa
     2881 gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa aaacagctaa
     2941 aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt aaagtttgtg
     3001 acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca aaatcttaca
     3061 gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag tttcggttct
     3121 ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca aaaaaaataa
     3181 ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct ggtcaccgtc
     3241 ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc cattataaca
     3301 actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc gattgattgt
     3361 ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc tacatcccgt
     3421 tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc acggctttct
     3481 gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt cacatacaca
     3541 cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac gccgatcaaa
     3601 ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat cacacagtta
     3661 ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac agctagggta
     3721 aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt ggttaagcgt
     3781 cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc atttgttgtg
     3841 atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt ttcattaggt
     3901 gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg ttaactgagc
     3961 gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc atttctcttt
     4021 cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt cgaacacttt
     4081 tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag tcgtagtaat
     4141 cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt tctgcaataa
     4201 tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt aattggtgat
     4261 agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacgtccatt ttaaacggta
     4321 cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa aaatcggctt
     4381 ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg ggttttctag
     4441 ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt ctaaatgtcg
     4501 tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat aaaaagccgc
     4561 ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt agacgatcca
     4621 tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct aaatttcttg
     4681 tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag ctttgcatgt
     4741 ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat ttacttggat
     4801 ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca tattcaaatt
     4861 catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata gtaataggat
     4921 ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc ttcacgaaat
     4981 gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca tggggaaaag
     5041 ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa gtcgtttcat
     5101 gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct attccatgac
     5161 catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc accaatcttt
     5221 gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa tttccatcgc
     5281 gaatagccaa caattctttc agtttatctt gcatttactt cccctccctt cgttacaaat
     5341 acactacatc aaatatatca tagtagttta tttgtaacaa gattaacgtc tatcgatcta
     5401 attcgatttc tttttgaatg cgctgctggt tctttaattg ttgcagtctt cttttatttg
     5461 cacgtaattt ctcttgttct aaacgaattt ttgctttttg attttcgatt tcttgttgtt
     5521 tgtgtgttgt atttcgcata ggataacccc ttcattattt tgtagtgcgg cgtgttcttt
     5581 tcttatagtt cgtttttttc ttttcagaaa attcaaagcc tgttgtaaag tcttctttta
     5641 aaacgatcgt tgcttcaaac gaacctttct gaccttttag tcctctgact gattggcttg
     5701 ttcggccgtt ttgaatgatc tctttcagtt gtgtggctgt gagtttcttt ttggcaatcg
     5761 ttgtccataa tttaaaatca caggcttcag acgtacatgc cgccacattt tgataaagca
     5821 agacttcttg ttcccgacat tttggacaaa cacctaaatg acgcgttgct tcctgttttt
     5881 cactggctgc tttcacctct tgtaacgttg atccaaaatc tgttgactgg atttgtcccg
     5941 gtacgacttc caacaagtga gagacaaatt tttggatatt agctaagaaa gtagcttgtg
     6001 ttcctttctg ctcaccaatc tttaataagt aactttccca tttggcggtc atttcggcac
     6061 ttgttagcag cggctctttt tccacggctt ggcaaagtaa aatcccttta tttgtcacca
     6121 caagcttatt tttttctacc ttgatatatt ccttttgttt caaagtttca ataatatttg
     6181 ctcgtgttgc ttctgtaccg attccttcta cttctttcaa tatggattgt gcttcttcgc
     6241 tatccacggt ttttccagct gttttcatcg ctgtgatcaa cgtaccttcg gtataaggtt
     6301 gtggtggctt cgtttctttt tcaaggactt gtagatctac cgtcaccgct tctccttttg
     6361 tcactttagg aagtagcggt tcttcttcct tttcttttct gtcgctctct ttgccaaaca
     6421 gctttttcca acctagatct actggtactt ttccgactgt ctttagctga agctgctgaa
     6481 cttgcgtcaa gatcgttgtt tcttcatagg tatacttttc cgcaaacatg gccaccgttg
     6541 tttttaacac ttgttcataa atagcctgct gcagtggact taattgttca aaagccgctg
     6601 ccgtcatgac cgtctttgta ggaatgatcg cgtaatgttc ctggactttc ttattattca
     6661 catatcgact attcggtgta tgaataggag taggaatggc ttcggccttt aaaaagtgtt
     6721 tgtattcgtc taagtgatcc aacagatacg catactcccc ttctgtaata taaggggtat
     6781 ctgttcgtgg gtagcttaga tatttccctt catacaaccc ttgcatggct tccaaagtat
     6841 ctttggcact agctttcatg agctgattca ttttagattg aaggctggat agagaaaaaa
     6901 gacgtggact tgccgcactt tttttctcgg tttcaacttg gtggatcgta cctggctgtt
     6961 ttcccaattg aacccccttt tcttttaaat aatcttcaaa tgcttcttgc gtcgcttggg
     7021 tttcattcgg atcaagcttc gcatcaaatg cgccattttg agcgattact tgggcttttc
     7081 cttcaaaata tggttctttt tggaagtttc taatcttttc ctgtaattga taaatcatgt
     7141 aaagcgttgg ggtttggact ctgcctaagg aaaaacttcc tggtatgcct ttttgctgca
     7201 agagtaaact atacagcggg ctaccgttca tgccgatcaa ccaatcggcg atctggcgtg
     7261 cttgtgcttc ttcaaatttt gggtaggtct cttctgcatc tcgtaactgc tggaatccct
     7321 gataaatggc ttctttttct aaggagttga tccaaagtct tttaaatatc tttccatgat
     7381 cggcgcctgc ttgttgaatg atcgtccaag ctatcagttc cccttcacga tcgctatctg
     7441 tcgcaataat aatggtgtcg gccgattgaa gttgctgttt caccaccttg aattgctttt
     7501 tcttatcttt aggaacatga tagtgataat gtgtagggaa aataggcaaa tgctctaatg
     7561 accattgttt ccaattttca tcgtacatat cgggcgaatc taactcgact aaatgaccaa
     7621 aaccatacgt gatcacagtt tctcctgaaa acaatcgatc ttggatctca aaatacccat
     7681 cttttcgggt agctttagaa aagctatcgg cataagcctt cgcttgtgac ggtttttctg
     7741 ctaaaataac ggttttcatc atgacgattt cactcccttt cttgctgtct accttgttga
     7801 aacaacatgg cacgtttttg ctttctgatc tcttgttggt agatttcatg cttcaattga
     7861 tcattccaat ccttttttac ttcaggtaca aattcatgtt catgattttt atagcctttg
     7921 tagtattgtt ctgctttata agagaaaaga atctgagcat taatatcccc tttttcttga
     7981 taaaaacgat caaaatcgta cgtaccatcc acgcgttcac aagcttttaa ttcttttgcg
     8041 acttctctac aaatatcttt caaattaata gcttctattg aatcgttaac agaaagctgc
     8101 tgaccaaaag acgcatttac tttgtgagcg gaaaccaatt ggttcgttaa ttgcagattg
     8161 ttttctgtct tgtgtatggc cataatagct tctggtgtga tcttttcttc ttttgcgact
     8221 tcttcataaa tgactttgta ttcttttggc acttgccaat cattagcgat tcctcgttca
     8281 caacgtactt tctgattcgt aaatggatca actgcgccca ttaattgttc tttttcgtag
     8341 aaaatatgtc ctgcacggtc attatccaca caaatctcga tggattgagg aaaggcttgt
     8401 ttctttctca attcagatac tgcctcacca aaataatgac tgatcacatg gtgttttaac
     8461 ccttccatcg aaactaacca cgtatcgttt aactgatcac gatttagtgc agcatagcta
     8521 agtaaatcaa tactactctc aaaaaatttt aactgcttag ggtcaccgat ctttaggtta
     8581 aagccatgat tcgctgtcga atttttatct atatgcttat atgtgcctct ctcaccaaaa
     8641 cgttcataat caatgaacgt tccttgataa ctcgccccta ctgtctcatt tgtctgtgga
     8701 tctttccata aaaaggcgac cgcatgatct aaacgatcgt ctcgaactaa gaaattttgc
     8761 cgttcatgtt ttggctttag ttcggcaatc aacccttctt tttctgcata atcaatcagc
     8821 cactctggta gttttcgttc ttccttcaga taattccttc ctttttctgt gagtggttga
     8881 taatcaacgg ggtgatcttt gtacttttct gtctcaaaat gaagattttc cactcgttct
     8941 gttttgtctt cattctttag aataaaatca gtggctgctt taaattgttg cttagggtct
     9001 tggatctctt tgtctatgaa aaaaagttta gctaattcaa taatgtcgcc actaatattt
     9061 tgactgttcc aataaaaacg ttgttttctt cgatcaaaaa caaatgaatc atggtcttct
     9121 aagcgatagt ctcgtccttt attgactaca gccattccat tgtttcgagc aaaatccact
     9181 atatcgatct gtttacatcg atcaataaga ctcatttttt tctcggtcat tttaaaacct
     9241 cccaaaagaa aaaagactat ccgtttttca gatagtcttt tttacttgtt ttatacacgt
     9301 tctttttgtg acaattgttg ttcataatag atttcatttt ttcgttttgc cacttctgac
     9361 actgtctcaa gtgaaaatcg gaatgaattt cctagcgcaa attcttctga aggtaacgcc
     9421 gtttggatct cttttccata actttttcct tttaaaatta cttgattatg ctgtggatct
     9481 tcaataaacg ccgcatcctt caaatgtaac gttcgacccg tatcttcttc cattactttc
     9541 ccgtctttct tgtcgatcca aagtgaacca acaggaacaa tattcttttg ttctgccgtt
     9601 tctacagtat ttttatgctc ctgcttcact tctgctggtt ggatctctgg ttctctttct
     9661 ctgtattgat tgatttcatt agaaatggtt tgaatgaatt catttgcgac acggctgaca
     9721 tcattcatga ttttcccacg ttccgcagct tccttatcat ccaacgtctg accattcttc
     9781 gtccatgctg caatataagg aatagctttc tccgaagtgt ccattccaaa atgtttgcac
     9841 acaatataag aagtcatttc tgcctgcaat tctttagttg cggtatctaa ttctgaaaat
     9901 ttgtgcattc ttgcatgggc taattcatga atacttgtag ctaaatgctg cgtaggagta
     9961 tttcgagtat tcatgacgat ttcttcttcg ccagtaaatt cgttacgaac atatgccccc
    10021 cgtgcttgtc ctaattctct ttgatagact tcactttctg tcatatctcg aatttgtatt
    10081 ccaatattgt tggctaccgc ttgaatccca gcttgaagtt ctgactgtcc ttttttatcc
    10141 aattgaaaat caaagacacg gttcggaaag attttcggat agtcttcagg ttttaactgt
    10201 gtttgtgtga tatcaaacac tttttcaatc gtgtagtaag gttttttgat cacttcgatt
    10261 tgaccacttt cgattcgttt tttatcttct ggtgtagctt ctgtttctcg aacaaagcca
    10321 cgtcctttct cataaaatcc tttgaccgtt tttctgacaa agatcttgtt cccttgttcc
    10381 ccttttttga tatagattcc ttcttttttc aacgcagcac gacctaaaca agccgttgca
    10441 cctggaaatt gttcctcaat caataattga tttctaaagg atcgttctgg aaaacgagac
    10501 ataaaattca aaagttcttt cacgtcttca ggagaatggg tatgcttttt caccgcatcc
    10561 agtgcctgtt gcgaaaggga tttgacttct tgcttaatct cttctgccgt tcgtttttga
    10621 tagccctttt gttttgtttt tgtttctttc gactgcattt gttctttcga aacaggttca
    10681 tttttgaccg tttgtttttg ttccttttca ggtacttgtt cgcacaattt tgctaactct
    10741 aaatagatgg gttgattact gttgacgcca tcccctagat ccattcggat ctgatcgact
    10801 acttctccgt tcttcacttc ttcaaaatag aatttataat aacctgcttc tgcggcgtat
    10861 tggttatcca acgttttgag ttgatcgatc aattctaatg ttacgatctc cccaccataa
    10921 cttttcaatc ctttttctgg atcattttcg ttgaattcta ctcgatagaa atgatcgttt
    10981 tctttctcct tcaaagaatc agttggcgca gcctcttcaa gtaattcacg aacagctgct
    11041 ttttcctctt ccgacgtggc ttggtatttt tttgattcct caaatccccc gttttcattc
    11101 agttctaaag caacttttcg atagtctttc aaaaaataaa tggttggatt ttttttccat
    11161 tgcgtcactc gtccgctgtc aataattgct tgtacttcag gaagaagatt ttcttttcct
    11221 ttttctgatc tttctttcag ctgttctaat gtttccaatt tccgttggta ttttcggatc
    11281 gtagcggctg aaaaagtcga ttcacctttt tcaaagcgtt cgatttcttc tttgattctt
    11341 ggaatatttt cgattgtcat atccaggccg ccgtacttgt tatagcccat ttcctttagc
    11401 tcttcttgtc tttcaagttt ttctacctgt ttttcttgtt gcttgatctc ttctctcaga
    11461 ctatgcactt tgttttctaa acgttcttgt tgcctaaacc aagaatgccc atttctctta
    11521 tcgttcattg gctgaccgtt ggtctttctc tggtgttcaa acatcttctc aatttgttcg
    11581 tttaattcat tttgcaatcg tactaatttt cttttggatt ttcccaagcg actattgtct
    11641 agtggttctt tttcttctgc catgtcc
//
LOCUS       DASJOX010000078        11548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJOX010000078 DASJOX010000000
VERSION     DASJOX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.079"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            404..1132
                     /locus_tag="U4A12_001961"
     CDS             404..1132
                     /locus_tag="U4A12_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1774314.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            1150..2346
                     /locus_tag="U4A12_001962"
     CDS             1150..2346
                     /locus_tag="U4A12_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1774315.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            2339..3334
                     /gene="lacD"
                     /locus_tag="U4A12_001963"
     CDS             2339..3334
                     /gene="lacD"
                     /locus_tag="U4A12_001963"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1774316.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            3331..4272
                     /locus_tag="U4A12_001964"
     CDS             3331..4272
                     /locus_tag="U4A12_001964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEN1774317.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            4448..4927
                     /locus_tag="U4A12_001965"
                     /pseudo
     CDS             4448..4927
                     /locus_tag="U4A12_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4940..5830
                     /locus_tag="U4A12_001966"
     CDS             4940..5830
                     /locus_tag="U4A12_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1774318.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            5817..6626
                     /locus_tag="U4A12_001967"
     CDS             5817..6626
                     /locus_tag="U4A12_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1774319.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            6640..7041
                     /locus_tag="U4A12_001968"
     CDS             6640..7041
                     /locus_tag="U4A12_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1774320.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(7087..8037)
                     /gene="yidC"
                     /locus_tag="U4A12_001969"
     CDS             complement(7087..8037)
                     /gene="yidC"
                     /locus_tag="U4A12_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1774321.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(8597..8872)
                     /locus_tag="U4A12_001970"
     CDS             complement(8597..8872)
                     /locus_tag="U4A12_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN1774322.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            8980..9744
                     /locus_tag="U4A12_001971"
     CDS             8980..9744
                     /locus_tag="U4A12_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN1774323.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9867..10370
                     /locus_tag="U4A12_001972"
     CDS             9867..10370
                     /locus_tag="U4A12_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1774324.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            10779..11420
                     /locus_tag="U4A12_001973"
     CDS             10779..11420
                     /locus_tag="U4A12_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEN1774325.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
BASE COUNT         3748 a         1927 c         2465 g         3408 t
ORIGIN      
        1 ttagttattt aaaaaaatgt ttgtcaaatt ttatagaaaa aaagtataaa actgcaaaag
       61 ttatctaaaa ctaataataa catttgtcat taataagcta tttttagttg ttgtaaatga
      121 tattggaagt aataaaacga tagttgtagg aatatcatag ggattaatgt ggatcacgct
      181 gctcgataag ttagaccatt ttgacggatc gcttatgtgg ttcgtctttt tttctgttct
      241 tttattggta actggtattt ttgaggtatc tttgtctatt caagatataa agctttaaag
      301 agctgttata atagcttttt tggtctaaaa aacctttgac aaatgattaa taattcacta
      361 ttcttgtgtt gtggttataa ccagtaaaga agaggtgatt tttatggaga gctcaaataa
      421 aggaaaatca ttgtattatc aactagttga tttattgaag gatcgtatcg aaacacagat
      481 gactccgcat gataagctgt tatctgaaag agaactttgt caacactatg gggtgagtcg
      541 taccaccgta cgtctggctt tacaggaatt ggaaaatcaa ggttatattt accgaagaca
      601 tggaaaaggt acctatgtct ctgatttgtc tgataagtca tcgaacttag ctggagcata
      661 tagctttaca gaagaaatgc gcgaaatagg gcgcgtccct aagacggtca ttttagaatt
      721 tagacaaatg gaagctaata agagaattgc taagaatttg aacatccatt taggtgaaac
      781 tgtatttaaa cttaaaagat tgcgtttggc agatgaagag ccattgatgg ttgagcgttc
      841 ttatctaccg gcgaaactat ttatgggact tgatttgccg ttacttgaaa gtaaaccgtt
      901 gtatgatgtg ttttcacagg attatgaaca acaagttcaa ttagctgatg aagagtttta
      961 tgcaagtatc gtgcaaaaaa aagatgctgt tgtgctacga atcgctgatg gcgctcccgt
     1021 cttacatctg acgaggataa cctataacgt gaaaaatgag attatcgaat atacagatag
     1081 tgtagcaaga gcggaccagt ttcattataa aattcggcat atccgaaaat gattgagagg
     1141 gagaacaaca tgtttgagct atcgaagaac aaattaaaac aaataggtgc agcaatcacg
     1201 acacaagaaa taaaacaaca accagatctc tggaaagaaa caatcgatag attgatgcaa
     1261 agccaagaaa atttagctct attttttgat caaataaaaa aagaagcaaa cggaagaaga
     1321 atacgtgtta tttttacggg tgcaggaacg tctcaatata ttggggatac gctcttaccc
     1381 tatctaaatg aacatggaga caatcagttt tttatcttcc acagttttgg cacgacagat
     1441 attgtagctt caccaaaaga atttttgttt ccaaatgaac cgactttatt agtttcattt
     1501 gctcgcagtg gcaacagtcc agaaagtgta gcggctgtgc aaattgcaaa tgaacaagtt
     1561 gaaacattgt ttcatttgac gattacttgt gctaaagagg ggatgttggc aaaaaacgca
     1621 caggatgata aacagaatta tctgttattg atgccagaga gagcaaacga tgcaggtttt
     1681 gctatgacag gaagttttac gtgtatgtta ttgagtgctt tatggatctt tgatcaaaca
     1741 gcaatggaga aaaaagaaag atatcttgaa caattagtta aaatcggtca agatcttctt
     1801 tcaagagatc aagaaattca aaagttatta cctgattttt ttgaacggat cgcttatctg
     1861 ggttcaggaa gtttggcagg tgcgacaaga gaagcacaat taaaaatttt agaattaaca
     1921 gcaggtaaag ttgctacaat ttttgattct tcgatggggt ttcgtcatgg accaaagtct
     1981 tttttaaata gcgagacact cgttgtaggt tttgtgagca atcaaccata tacgcgaaat
     2041 tatgacttag atattttaga ggaaataaaa gaagatcggg tcactgccaa tgtcattgcg
     2101 attgcgcaac cgggtactat tgatttctca ggtatgactg tgctattgga agactcggtg
     2161 agtaaattgc ccgatgccta tcttgcatta gcgatgatta tgactgcaca aatcattgca
     2221 ctctatactt caatcaaagt agctaataaa ccagatacac catctccaac aggcacggtc
     2281 aatcgagtag tcaagggcgt cattattcat aagtatgaaa ccgaaggaga aatgacagat
     2341 gaatgaagaa aaaaataggg caatggagag actatcaaca aaagatggga ttattagtgc
     2401 attagcgatc gatcagcgtg gtgctctaaa aaaaatgatc aaatcgttgg gaaaagagcc
     2461 aagtgaagac caaatcagtg aattcaaaga actagtatca gctgagctga caccctatgc
     2521 ttctgctatt ttattggatc cggaatatgg attgccagca gcaaaagtca gagcaaaaga
     2581 gacggggtta ttgttagctt atgagaaaac cggctatgac gcaactacgc ctggtcgtct
     2641 acctgattta ttagcggatt ggtcttctct cagattgaaa gaacagcaag cggatgcagt
     2701 aaaattctta ctttactacg atgttgatga agcacccgaa atcaatcatt tgaagcatgt
     2761 gttcatcgaa cgtttaggta gtgagtgcga aggagaaaat cgtcccttct atttggagtt
     2821 agtttcttat gatgcacaaa taagcgatag ttcttctcca gagtatgcga aagtcaagcc
     2881 ccacaaagtt attgagatga tgaaagaatt ttctgatcca aaatataaag ttgatgtgtt
     2941 gaaagtagaa gtaccagtca atatgaatta tgtgaatggc tattccacag gtgaatttat
     3001 tcacagcaaa gaagaggctg ctgccttctt taaggagcaa agtgaagcga ctccattacc
     3061 attcattttt ttgagtgggg gtgtttcaac agaattattt caacaaacac tgatttttgc
     3121 tcatgaatcg ggttccacct tcaatggtgt attgtgtgga cgtgcgacat ggaaagatgg
     3181 ggtcaaagca tacgtcgaaa atggaaaaga cggagctatt cattggttaa gaacaaatgg
     3241 gagagaaaac atcgagactt taaataaagt aataaatgaa accgctaccc cttggttcaa
     3301 taaaacaaaa agcaaaaagg tgaagatgaa atgattacga cagttacctt gaatccttcc
     3361 atcgatatcg cgtatacaat tgaagaatta aacctcgaca aagtcaatcg agttgaaaca
     3421 gtcaataaaa cagcgggagg aaaaggagtg aatgtggcga gggttctaaa tttattagga
     3481 gaagaggtga tagctactgg tttaataggt ggccagcacg gtgaatatct aaagaaccag
     3541 ctagatcagt tagggatcat gcatcagttt gttccaatta aaggagagac aagggattct
     3601 attgctatcc tgcatagttc gagacaaaca gagcttttgg aacgaggccc cttcataaca
     3661 aagaaagaac aacagacatt taccgatcat ttcgagcagc tcctcgaaat atcagatatt
     3721 atgacgatct caggtagttt accccaagga tgccctcttg attactataa gcaattggtt
     3781 gctttagctg ataaaaaagg gaaagacata ttactagata cttctggtaa atttttgctt
     3841 gaagtattgg aaggtaagga gaagccacta cttatcaagc ccaatctgga agaattagga
     3901 gaactgattg gccaacgtat ctgttcttcg attcaattag aaaaaattca gtccttatta
     3961 gctcatccaa tatttgaagg agtgccatgg gttgttgttt ctttaggaga gagaggtgct
     4021 gtggcaaaag ttgctcagtg tttttacgaa gtcattattc ctaaaatatc agttgttaat
     4081 cctgttggtt caggtgacgc gacccttgca ggatcggccc atgcgttatc aaaaaggcaa
     4141 tcaatcgaag agattctcgc attttctatg gcttgtggta tggcaaacgc gcaacaagag
     4201 aaaactggct ggatcgaccc tatgaaagtt acgatgttga tgaaacacat tcaagttaag
     4261 aaaatcaaat gaacttaaat ataaaagaaa gcgttttctt tagttgggaa ggaatttgtt
     4321 cttagcttaa aaatgaaata taagaaagaa aaaatgaaaa cgttatcttt gaaacagaag
     4381 ggatatttga tgatgtcaca gttttaattg aacaataaat agaaaaaata gataaggaga
     4441 atgcatgatg gcaattattg gtgtaagaat cgatggacgt ttgatacatg gataagtagc
     4501 aaatctttgg acaacgaaac tgaatattag tcgaatcatg gtcgtggatg atgaggtagc
     4561 cacaaacaca attgaaaaaa gtggattgaa gttagcgact ccagcagggg taaaattaag
     4621 cattttgcca atcgaaaaag cagcaacaaa tattttagct ggcaaatatg actctcaacg
     4681 agtattgatc gtagcaaaaa agccagatcg aatcttatct ttagtagaaa aaggagttcc
     4741 tatcaaagaa gtaaatgtgg ggaatatgtc acaaacgaat gaaacacggt ccattacaaa
     4801 atcaatcaat gtggtagatg cagatatcga agtatttaaa aaattagatc agcttggtat
     4861 acatttaatt gctcaaatgg taccaaacga taaagcagaa gactttatga atcttttaat
     4921 caaataggag gaatgatcca tggatattca atggtggcag atcttattat taacattata
     4981 tgcaggttat caaattttag atgaattaca gatctattct tcaatgagtg cgccagtgtt
     5041 tgcagggtta tatgcaggtt tagtaatggg tgatttaaac gcaggtttga ttattggtgg
     5101 cagtatgcag ttgacagttt taggagtggg gacgtttgga ggcgcatcaa aaatagatgc
     5161 caactctggg acgattttag caactgcctt ctcgatttct cttggaatga atccagaaca
     5221 agcgattgct gctattgcag tgcctgtagc aagcttaatg atccaattgg atattttagc
     5281 tagattcgct aacacatact ttgcccatcg tattgaccgt cacgtagaag acatgaatta
     5341 tcaagcaatc gaacgcaatt tcttaatggg agctttacct tggtcgctct ctcgtatgat
     5401 tcctgtattt ttagctttag catttggtgg tggattagtt gaaaatgttg tttctgtttt
     5461 aaacgggaat ctaaaatggc taggcgatgg actttcagta gcaggagctg ttttaccagc
     5521 tgttgggttt gccattcttc tgcgctattt accagtaaaa aaacatctat cttatcttat
     5581 tttaggattt acgatcacgg cattattagc aacgttattc tctaatgttc aattattagg
     5641 aacaagtgtg gcaagcgttg ttaaagatct tccggcaact ttcaattcgc tgccgatgct
     5701 tgctgtagca ttgattggtc tagcactagc aatgatcagt tataagaatg atcaaaacaa
     5761 agaccatcaa ccattagcta gtgagccatc acattctgtt gagggggaaa tcgaagatga
     5821 cgaaatctaa ttacaaatta acaaaagaag attttaagca aatcaatcga agaagtttgt
     5881 tcacttttca attaggttgg aactatgaga gaatgcaggg ttctggctat ttatatacta
     5941 tcttgccaca actaagaaaa atatatggag acaatacacc agaattaaaa gagatgatga
     6001 aaacacatac gcaatttttt aatacttcta atttttttaa tacaatcatt actgggattg
     6061 atcttgcaat ggaagaaaaa gagggaattg cttcaaagga aaccgtatcg gggctgaaag
     6121 ctggattgat gggttccttt gcagcgattg gagattccgt atttgctgct ttgattccaa
     6181 cgatttttgg ggctttggcg gcgaatatgg caactgatgg aaatccgata ggtgtgttta
     6241 tatggatttt tgctcagatc gttgtcatgg tttttcgttg gaagcaactt gaatttgcat
     6301 acaaagaagg aatttcctta gttactacta tgcaatctcg cttaactgca ttaacggacg
     6361 cagccacttt attaggggta ttcatggtag gggcattagt tgcgacaatg atcaatgtta
     6421 aattttcatg ggcaccaacg attggcgatg tgacattgaa tatgcaaaac tatatcgata
     6481 tgatcttacc taaaatttta cctgctggga tcgtcggtgg tgtttattgg ttattaggaa
     6541 agaaaaacat gactcctact cgagcgattt ttatcgtatt aattgtgtgt gttgctttat
     6601 ctgcattggg tgtgatttcg aaataaggga gagcgaaaaa tgagtagaca attggtattg
     6661 attagtcacg gaaaattttg tgaggaattg aaaaaaagta cagaaatgat catggggcct
     6721 caagaggata tctacacgtt acctttatta ccagaagatg gcactgaaac atacaaacaa
     6781 aagtttctca ctgtcacaga ttcgttaaat gattttgtgg tactatgcga tcttttaggt
     6841 ggtacacctg caaatatcat ttctaaacta ataatggaag gacaacagat tactctttat
     6901 gctgggatga atcttccgat ggtcattgaa tttatcaact cacaaatgat tggttcagag
     6961 cccgagtttg ttcgtgcagg atgtgaaagc attgtctctg tcaacgaatt aattaatgga
     7021 atagcagaag aggaagagta acaaaaaaga tgagggctta gctctcatct tttttgtcct
     7081 atgtttttat ttccgcttct gctgctttcc tgcgttgcgg cctttgttgt ttgtactgtt
     7141 ttttggttgt ttgatttgtt ttgttgcttg tttgttgtta gctggctttg ctgattctgt
     7201 cacgtctttt cttggagtga cgacttgttt tggcgggttt tgtttcaact cttctgccac
     7261 ttgtgctttc aaacgtggtt tcatcataac gtttgtgatg aatgtttgca gacagctgaa
     7321 cacaccaccg actacccagt aaagtgtaac accggctggc gcactgatcg acatgaagac
     7381 gatcatagca ggagagacga ttagcatcgt acgcatcgtc tttttctgtt cttcaggaat
     7441 accgatcgtt gagatgtatc cttgaagcag ataggcgacc cctgcaatga caacaagtac
     7501 catacttggt gagcctaaat cgatgccgta gaaagatgat tcccgaatac cttctgtata
     7561 acgtgccgta aagtacaagg cagagaagat cggcatttgg atcaatagag gcagacagcc
     7621 gattccgccg gtcatactca atccgttttc gcggtatact tgctgcattt ctgcttgtgc
     7681 tgccatttgt tcttcacgtg ttgttgcagc tttcaatttt tgttgggctg cttcaacttg
     7741 tggttttaac gcttgcattt tttcagattg gatcattgtt tttttcgatt gactaatacc
     7801 tagtggcaag atgatgatac ggacgatcac agtcattgca ataactgccc agccataaga
     7861 ccaattgaaa ttctcaacta gatatgtaat cgcttggccc ataggatgga cgagtactcg
     7921 ataaacgatt cccgaagtgt cagggtttcc gtttgaatcc atccgcacac agccagataa
     7981 aaagagcagg atactgacaa gtccggatcc caataaccag tttttccatt tattcataga
     8041 atacttcctc ttctataatt tttacacaaa cttaactata ctaaaaaagc gcatggaatt
     8101 caatcaactt catagaaatt tcatataaat attcgttctt ttacgtagcg aaaaattttg
     8161 aaaaaaagac aaaaaatcaa gaaagaaata cttggaaaga aaagagtgat tccaagtgtt
     8221 tctttcttat ttaaaaaact aaaaactttt ttgttctgtc tggaaaatat gggttaagca
     8281 aatttattgc aagttataga tcagcttttt tactacctat ctttaatact tgaggcaaag
     8341 cgtctcaaaa acaaccttat cgacggtgtt caaaagtcag tttctcggta ttaagtcaaa
     8401 ttttatgcaa aaatgtggaa agccattttc acaaaattct ccttactact caaaactaag
     8461 cgactttctc acaaccttat tgacggtgtt caaaagcttg catctacggc attaagtcca
     8521 gcctgttgaa aaatatggag agctattttt cacaacctgt ccttaatgct tgatgcagac
     8581 cagctttttc acaaccttat cccaccacat caaatttctt tcgttcctca atcatcgggt
     8641 cttcttggac atccacatac tgcactcgtc cacttgggct cggagatttc ttgacttctt
     8701 caataaattt ctggatgatg tcgtcattgc cgattgcttc gatcgtgact gagccgtcat
     8761 cttcgttttt taccgttcca gtcacgccta gttgatcagc gaccatcttg gtcatgtagc
     8821 gaaatcctac gccttggacg cgtccttgga cgttcatttt aactttgcgc atgttcgtca
     8881 cctttctttc ctattttata aattaatgat accgattttt ttaggatgaa acaagctcaa
     8941 gcacctattc catgatataa tcatggggag gtgaggaatg tgaaagagat ccaatcagca
     9001 aaaaatccaa gaatcaaaga attaaaaaaa ctacataaaa agaaatatcg ggaagaaaag
     9061 gaagaatatc ttttggaagg gttccatctg attgaagaag ctgcgtcgtc tgatgcagaa
     9121 ataaaagaac tttttgtgac acagcgaggc atgaaagaat ggggagactg gctggccgca
     9181 aacaaagaga tttctacttt tctagtttcg gatgaggtca tggatgctgt atcggatcta
     9241 cctacaccac aaggaatctt agcagtgatg gcgttaacgg ctgaaggcac gccagatttt
     9301 tcaggaggat ggctgttatt agataatgta caagatcctg gaaatgtagg gacgatgatc
     9361 cgtacggcag atgcatttga tatggctggt gttttattag gcaaaggaac ggcagatatt
     9421 tatagtacaa aggtgctgcg cagcatgcag ggaagcaatt atcatttgcc tgtgttgcaa
     9481 aaagatttga cagaagtgat tcctttaatg aaagacaaag ggtttacagt gtatggcaca
     9541 gaattaaatg aagaagcaca cgccttagac aaagtagaaa aatcagataa cttcgcaata
     9601 atcatgggga acgaaggtca gggggtaagt caggaaatct tgtctttgac tgatcagaac
     9661 gtatatatcc cgatgaaagg aaatgcggaa tcgttgaatg ttggggttgc ggcagggatt
     9721 gttctttatc atttttcttt atgattaagg aactattaag ctttggctat tttatccaag
     9781 gcgactagaa taatccgaac actggtggta tacttagatc aatttcagaa gaaaatgaaa
     9841 ttcaaaaagg tggcagatcg ataattatga cagaaaaatg gcgtgaagac gaagaatata
     9901 tgtcttatgt agaagatctt ttagaaacag aagcagtgaa aaaattagcg aattacacac
     9961 agcatgttca ttccactcgt ttggagcatt ccatcagtgt ttcttactat agttatctgc
    10021 tcgcaaaaaa atggggtggc aacgctaaag caacagcgcg tgctggactt ttgcatgatc
    10081 tgttctacta tgattggcgt acgacgaaat ttgatgaagg cacacatgcg tatatccatc
    10141 caagaatcgc tgtaaagaat gcagaaaaga tcaccgattt gtcggatctt gagcgagata
    10201 tcattttgaa gcacatgtgg ggcgcaacga ttgcaccgcc taagtacaaa gaaggatata
    10261 tcgtaacatt cgtagacaaa tactgcgcag tcaaagaagc cgctcagcct atgtcagcaa
    10321 gtatgcgtaa gagatggcaa cgttacttcg gaaaagaatc ctcgatttaa caggtaaaac
    10381 caataataga ccatattgag gttgtgacag aagtgggtca gcttcaagaa ataagaagga
    10441 atttacgaaa attgcttctc aaatttttgt gaaattccgg cttatttttt gaagaagcta
    10501 cttctggaac accgtttgtt tgtagaaagg gactttgaca cgacttttgt cacagtcctt
    10561 tttctgtttg cagttgacta tctgctcaag atacggttta cttttattta tcgaatgaat
    10621 catgtacata gaagaatgaa cattaaaggg ggtgatgata agatgcaaaa cgtatcttat
    10681 ttaaaagagt ccgggatctt caggtaacac atactttgca gaagatcact tttttctaca
    10741 aagtatgcac tttggttaga agaaaggact ttaaaatcat ggaacaaaca atgaaaccat
    10801 atcaatattt ttttataaaa agagaagtag agcagttatt aaatgcgtac tcatctgtca
    10861 atgactcaaa aacggtacaa acggttcagg ctctagcagc tgaaaagatt cgcgatgtat
    10921 taggttacga accatcagaa atcgatacgt ttttaacgac agttttagat gtaaaactga
    10981 caaaaacgca agcagaacga gcattggagg agctgaagca aatcgtccag ccgtttatgc
    11041 agccgagcaa accacaaatc gagaaattat tccgaaaagt gaaaaaactc aaacaacccg
    11101 cttggaatga aatcgacttg caagcacata cgtacatcgg ttggaatgat ccagggacgc
    11161 aaaaaaaatt catggtttat tacgaagatg gaaaattaaa agggatcagc ggtactttat
    11221 caccaacgat taataaggga atctgtgcga tctgtcagaa aacatcgaat gtctcgctct
    11281 ttctttctac aacgaaagcc ggttctgatg gcacgtatac gaaaaagggg aattatattt
    11341 gtcatgatag tgatcaatgt aatcaacagt taacgaaatt ggctcctttc tatgaatttg
    11401 ctgaacgtgt aaagaaataa cgataatttt attgtttttt acagaaaaat atgctatctt
    11461 ttaatggaag tgaataggtg atggtgttcg cctttaaatg ctaacgtaag atagctaatg
    11521 acgcctacta aagtctgggg ccagcccc
//
LOCUS       DASJOX010000079        11417 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJOX010000079 DASJOX010000000
VERSION     DASJOX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.080"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(19..564)
                     /locus_tag="U4A12_001974"
     CDS             complement(19..564)
                     /locus_tag="U4A12_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1774326.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(576..1268)
                     /locus_tag="U4A12_001975"
     CDS             complement(576..1268)
                     /locus_tag="U4A12_001975"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN1774327.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1287..1571)
                     /locus_tag="U4A12_001976"
     CDS             complement(1287..1571)
                     /locus_tag="U4A12_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774328.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1584..2147)
                     /locus_tag="U4A12_001977"
     CDS             complement(1584..2147)
                     /locus_tag="U4A12_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1774329.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2375..3037
                     /locus_tag="U4A12_001978"
     CDS             2375..3037
                     /locus_tag="U4A12_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN1774330.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3161..4357
                     /locus_tag="U4A12_001979"
     CDS             3161..4357
                     /locus_tag="U4A12_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN1774331.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4458..5834)
                     /locus_tag="U4A12_001980"
     CDS             complement(4458..5834)
                     /locus_tag="U4A12_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1774332.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5871..6737)
                     /locus_tag="U4A12_001981"
     CDS             complement(5871..6737)
                     /locus_tag="U4A12_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN1774333.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6826..7851)
                     /locus_tag="U4A12_001982"
     CDS             complement(6826..7851)
                     /locus_tag="U4A12_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1774334.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7869..8543)
                     /locus_tag="U4A12_001983"
     CDS             complement(7869..8543)
                     /locus_tag="U4A12_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1774335.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8727..9620)
                     /locus_tag="U4A12_001984"
     CDS             complement(8727..9620)
                     /locus_tag="U4A12_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1774336.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9694..11391)
                     /locus_tag="U4A12_001985"
     CDS             complement(9694..11391)
                     /locus_tag="U4A12_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1774337.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3383 a         2514 c         1963 g         3557 t
ORIGIN      
        1 tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa aatgtctcaa
       61 tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa tatggagttt
      121 gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg tacatacgcc
      181 ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat ctgttccact
      241 aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct catagtcttg
      301 gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca cattatgcgg
      361 aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat caatggcaaa
      421 aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct ttttttcaat
      481 tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat catatgtgta
      541 gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt gacaaattcg
      601 atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa gctttgtgtc
      661 gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat attgaactga
      721 cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc agggaaattc
      781 gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt aacgatcaat
      841 ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact gcgcaaatat
      901 gtgcttgctt cataatatag aggcattcct ggtaattgtc ctggtttgta gccaaatcct
      961 gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc tacttgtaag
     1021 ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc gtattgttgg
     1081 atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg tacgacgatc
     1141 acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc ccatgataat
     1201 ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc gataataccg
     1261 attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa aaccacacat
     1321 aacattagga taacaataat aagagatttc atcaaaaaag agttccattt ggtcgtcttt
     1381 tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc tttacgataa
     1441 aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg ctgttttttt
     1501 aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt aataagcggt
     1561 cctttggcca taagccgatc cctctacttt cctttggtca atagttccca gtattgaatc
     1621 gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg cgcctcttca
     1681 agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg attctcgacc
     1741 ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc tggtgaaaga
     1801 tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc ttctaattct
     1861 ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc agctggaatc
     1921 tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag acgatcttgt
     1981 tcgtcaaaag caatgatccc gacaccacca ggatgaaata ccagttctct cttcgaagtt
     2041 cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc gctatagatt
     2101 tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc ttctcctgct
     2161 ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa actccttatt
     2221 agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc tttagtccga
     2281 tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg aaaagaattc
     2341 aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt ggccgtggct
     2401 tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt ttgttctagg
     2461 acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc gcaggaaaaa
     2521 gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact atgaaaaaag
     2581 cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa caaaaaatga
     2641 gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa tcgatctccg
     2701 ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag aaccaaaccg
     2761 actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg atttgacaaa
     2821 taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg aaaaactaga
     2881 agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt atcaaaaatt
     2941 cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac agagcttaaa
     3001 gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat aaaagaagac
     3061 tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta gagacattca
     3121 tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata aaccgactga
     3181 aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca acccgttttc
     3241 aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc tccaagagca
     3301 acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac aagctaaaca
     3361 gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg gatcggctgc
     3421 ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag cacaagatat
     3481 tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag ccaatcctga
     3541 cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg tcaaacaatc
     3601 gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca agatttcagt
     3661 gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag aacagcttta
     3721 ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg gagaattgaa
     3781 aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag aagaaactgg
     3841 cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc gtctggataa
     3901 acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac cacaaatccg
     3961 tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta tcgctacagc
     4021 cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc aaaaagatgc
     4081 agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga aaaattcaga
     4141 aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa tcgtggatat
     4201 cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga cattgcgtat
     4261 ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac acatggaaga
     4321 agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct tgttactaaa
     4381 acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta tctttcttct
     4441 aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc atagccaaaa
     4501 taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac agtttgcaga
     4561 cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg aacgaatggc
     4621 actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag taagcctaga
     4681 ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa tacattcaat
     4741 gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc ttttttcggt
     4801 gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc cttgctgaca
     4861 gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag tagcgttaaa
     4921 attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct cattgcaaaa
     4981 gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa gatcagcgtc
     5041 acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct tggcacatct
     5101 ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc caagaaagca
     5161 aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat gaatggctgc
     5221 cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat gataccaaat
     5281 ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag cgacacgatg
     5341 taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc attttccgga
     5401 ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa aaaaccttgc
     5461 atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc cccgatcaac
     5521 aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata ggcatatggc
     5581 ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga caaaatgaca
     5641 acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg attggccgca
     5701 acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag catgatcaaa
     5761 tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat ctctttctta
     5821 cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga ttaaactgtt
     5881 ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac aataacataa
     5941 atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa agtagagata
     6001 atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt gactgttaga
     6061 agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt ccacacaaac
     6121 tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat tccgatactt
     6181 tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat ggtatataca
     6241 ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc aagcatttta
     6301 ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca
     6361 agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca ttcatggaag
     6421 aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa tggtagccct
     6481 acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac actccaagct
     6541 aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact aacaacgaaa
     6601 aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg ttgattggca
     6661 tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat agggactaat
     6721 gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa cttatcagcg
     6781 aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac gtttttcatt
     6841 ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt cagttgattc
     6901 agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa gtcctaatcc
     6961 tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat caaagatccg
     7021 tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc tgactccttg
     7081 cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt
     7141 actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa cagtatgatc
     7201 ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct gactgcgtat
     7261 cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc gcgagaaact
     7321 gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg ataattcatt
     7381 ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt
     7441 agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat ctttttggtc
     7501 ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca gctgattgat
     7561 atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat tttgcaaagg
     7621 ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca agtaaccacc
     7681 cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa ttgtgctcaa
     7741 atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa taaaaaacag
     7801 ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca tcttaaccgc
     7861 tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata tccttggata
     7921 cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct
     7981 acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa gatctttcca
     8041 ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc aatgatctct
     8101 ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt
     8161 tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat ttttgcaatc
     8221 agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt catagccatg
     8281 atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt cgatacttca
     8341 cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc tagtaataca
     8401 agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt cgttccaaag
     8461 gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct
     8521 tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc tactacaata
     8581 tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt agcaaacgtt
     8641 aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag cttaaattta
     8701 ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg tttttgtctt
     8761 ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat acctagaagg
     8821 agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga agagacttct
     8881 tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt
     8941 gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt atttgttttt
     9001 gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc atacacttca
     9061 agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt tttctcagca
     9121 ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg aaaagaggtg
     9181 ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc aaataaaggc
     9241 aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc gtaatattct
     9301 tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac ttgtcccatt
     9361 cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac tgctaattgt
     9421 tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc gatccttctt
     9481 tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac tgcagctccg
     9541 gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt ttgaggtcta
     9601 ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc agcaaccttt
     9661 ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca ttttagattt
     9721 acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga atgccaagag
     9781 cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc tttctggccc
     9841 tggattttct actcctacga tctgaatttc ttcattatag cttccataag agatgataaa
     9901 ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca tggtctcttt
     9961 catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc catctgcgac
    10021 aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg attcataaac
    10081 agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt cattccacaa
    10141 ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt caaactttct
    10201 tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa attcatcatt
    10261 tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta gttctgtgga
    10321 aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta atctacctaa
    10381 agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa acccgttgct
    10441 tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc
    10501 tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt tgtaattttt
    10561 gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag gtgctgtgat
    10621 cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt cttgttctcc
    10681 agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca tctgtttatc
    10741 tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg tgtctttcca
    10801 tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca tcataacaac
    10861 cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga aaagatagac
    10921 tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata ccgcttctcc
    10981 agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa accaactttc
    11041 tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc
    11101 cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt caaacaaatc
    11161 aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc cgctattcgg
    11221 tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg
    11281 tttttgtgcg acttcttcgg ctttggctag ttttccgttc ccgaaaatcc ctattcccgc
    11341 agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca tttttttctc
    11401 cccgttcttt ttttatt
//
LOCUS       DASJOX010000080        11010 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJOX010000080 DASJOX010000000
VERSION     DASJOX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.081"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(23..1066)
                     /locus_tag="U4A12_001986"
     CDS             complement(23..1066)
                     /locus_tag="U4A12_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN1774338.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(1056..1547)
                     /gene="coaD"
                     /locus_tag="U4A12_001987"
     CDS             complement(1056..1547)
                     /gene="coaD"
                     /locus_tag="U4A12_001987"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN1774339.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1544..2104)
                     /gene="rsmD"
                     /locus_tag="U4A12_001988"
     CDS             complement(1544..2104)
                     /gene="rsmD"
                     /locus_tag="U4A12_001988"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN1774340.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2223..2561)
                     /locus_tag="U4A12_001989"
     CDS             complement(2223..2561)
                     /locus_tag="U4A12_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN1774341.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2545..2982)
                     /locus_tag="U4A12_001990"
     CDS             complement(2545..2982)
                     /locus_tag="U4A12_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1774342.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(2972..4096)
                     /locus_tag="U4A12_001991"
     CDS             complement(2972..4096)
                     /locus_tag="U4A12_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774343.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4117..7545)
                     /locus_tag="U4A12_001992"
     CDS             complement(4117..7545)
                     /locus_tag="U4A12_001992"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN1774344.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7571..8734)
                     /locus_tag="U4A12_001993"
     CDS             complement(7571..8734)
                     /locus_tag="U4A12_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1774345.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8737..9039)
                     /locus_tag="U4A12_001994"
     CDS             complement(8737..9039)
                     /locus_tag="U4A12_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN1774346.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9153..10925)
                     /gene="recQ"
                     /locus_tag="U4A12_001995"
     CDS             complement(9153..10925)
                     /gene="recQ"
                     /locus_tag="U4A12_001995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN1774347.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         2993 a         2418 c         1879 g         3720 t
ORIGIN      
        1 acccctcttt aattatttag tttcatttca ttttctctag atagtcaagc gcttcttgta
       61 ctgtttttac tggtacgatt ttcattgagg aattcaattt tttagcggca gcttttgctt
      121 cttcgtagtt ggtttttatt tcaggatatt ctttcttaac atcgtctgaa atcgtatcat
      181 ctggtgcaaa gaaaatctct acgccagctt tatccgcact tgccactttt ttatcgattc
      241 ctccgattcg gccaacttct ccttcactgt tgatcgttcc agttcctgca atttgcttac
      301 catgtcgcaa gtttttacca gtgacttgtt catagatttc taaagtgaac atcaatcccg
      361 ctgatggccc accgatagag ccagaattaa tacgaacatc tgtatctgat tcgatctcgg
      421 tatggtcagt aagcgtgata cctattcctg cctttttatc tgttggtaac tcgatcagat
      481 caccagaagc tgttttttct tcaccattat gcaaataact gatttcgatt gtttgtccaa
      541 ctttttggct ttttacataa tccataaatg cttctgtact ttgaaagctt ttcccgtcta
      601 cttttgttac tgtatctcca actgaaagct tgccaaagaa attcgatgtt ttctctacac
      661 tcatgacata tacgcctttg aattccattt tataaggctt gtctgcaagt tttaacgctt
      721 gttcgattgc tgcattttgg gaagaatcca tataatactg ttgcatttga ttatattctt
      781 catttgtcgc cccaccagtc aattcttctt cactgatcaa ttcttcaaaa ggggtcattt
      841 tagcttttaa agctaagaaa cctgtagctt gcctgatacc aacagaggta agagagaaag
      901 acccagaacc tttatcttcc ttatcattga ccgtaattag ttctttcaga tcgatcgtgg
      961 ctcccggtga ttctacataa tacggcaagg gaactactac aaatccaatg atcaacattc
     1021 caaatatgat aatcagtact gttttcaacc attgtttagc tttcatttga ttcacgcttt
     1081 ccagctaaag cttcgttgat cactggcggt aagtagctgc tcacatctcc gccaaagtgc
     1141 agtacttctt ttaaaatact ggaactcacg tgactatatt ctggttttgc taataagaag
     1201 acagtttcca attcatcata tagatgatga ttcatctgag caatctctcg ttcatattca
     1261 aagtctttga tacttcgtat gcctctgatc aacgcatctg caccaatctc ttttgctgtt
     1321 tctaccgtca attgattttc ttgatgcatg acttttacat taggaagatg agaaacagct
     1381 tttgtgatca atgtcattct ttcttctggg ggaaacaaac ttttctttga agtattgata
     1441 aaaacaccaa ttactacctc atcgaataat ttagccgcac gttccaccgt atctaagtga
     1501 ccttttgtaa aaggatcaaa acttcctggg aataatgcac gtttcattga tttccctctt
     1561 ttctatagat agttacggcc gttatgccat aaatctgttt tcttatttga tggaatccag
     1621 ccacagtttc tggcaattcg actgttttat ccgtttcgca tacgataact gcggttttag
     1681 aaaaaagtcc cagttcttcc attttatgga tttgatcgat tattttttgt tttgcatatg
     1741 gcggatcaag aaacaagtag tcgaattgtt gtttctccgc tgctagccgt tcgattgctt
     1801 tgtccgcatc aagttttatt gtggtaaatt tctctggttc tttcgtaatt tcgatgtttt
     1861 ctttgatgat ttttagcgct tggtaatttt tgtctacgca taccgcatga ctgcagcctc
     1921 tagatactgc ttcgatagct aaaccaccgc ttcctgcaaa cagatccaat actgtctctc
     1981 catcaaaata tggtccaatc atattgaata tggattcttt tactttatct gtagttggtc
     2041 tggtgttcgc ccctgctaaa ctttttaaac ggcgcccgcc ataatttcct gatattatcc
     2101 gcatctactc actccttaga aatggtactg gtactagtat accaaaaaaa tacttggaca
     2161 atgatcaaaa atcgatcgtt atccaagtta ttttttattt aaaaactgct cataagagat
     2221 ttttaaagat gttcagctaa tcggatttct gtatttagct cttcaatttc gaaaccttcg
     2281 tcttttaata ctgttttgtt tcctattttt tcggcaaaat tcatttcgat atccggtcgg
     2341 taagaacgtt ctacgtttcg aacaaaatgt aatttacgga ttcgttcttc tgtttcttcc
     2401 aatgtctctt catttaagta aagcacgaca tatttcattc ttcttgagac ataatgcaac
     2461 aagccgaact ttttcagatt ctttaattgt tttaagctgt agacccatac gatcagcccg
     2521 cgtcgttttg ttatttcaaa ttcactattg tctaattgca tgacaattac ctccgcatcc
     2581 aaagtgtttt ttcgtttcaa aaaacgggct acctgcatct actttgattt cattagaaac
     2641 ttcttcggca agtacttgga caatttgatc caacagactt tgcagctgtg tttcactata
     2701 tcggaaagct gcaacatgct catccatatc aagctgtctt tttgcttttc gaacagctcg
     2761 ttttttttca cgataatcag gtgcatactc accatatgcc gctacatttt caaatgcttc
     2821 tttcttagac aagaacgctt ctttcaactg gcaaactttt gcagattctt ccattgactg
     2881 tttatgttca agataagaag ccatcgtttt actctttttt atcatttcag cgacttggag
     2941 gcattgatcc tccagttgaa acagctcttc gttaacgatc actgctatca ctactttctc
     3001 cgttttcttc ttgcaataag actaacatat tttctttaga aaaagcaatg cctacagaat
     3061 ccgttcccgg gtccatgaaa cggtcatggt tgtaaggatc gctgtaccaa tataaaaacg
     3121 aaaaagtagc gtcaatcgcc ggatgaataa atagcccact agtattttct aatttttgtt
     3181 tactgattaa cttctccaat tcatcattcg tcagtgagaa tttcactgtc gtttgagcac
     3241 tgcctagcga tttttgccaa tcagccatac gttcctctga caatatttcc tcaggccttg
     3301 tcaaaaaatc atgtagtagc agctgactgt ggttttgaaa ggcaagtgtt tgattgtata
     3361 tgggaagttc gtcttctctt ctaagcatat ttaataaagt cgtacttttt cgttctttaa
     3421 cgcgattgat tgattcccaa tcagccgatt tatctgcttg ataatgtgga aggccttcac
     3481 caaacacttg atatggcaat agtttcaaaa gactatcttt atctaaatac acaactgaaa
     3541 ataggccgtt tgttgtttga tcaaaaaata aaatagcaaa agtgtcgtta tcaaaagcaa
     3601 ttagcggacg ataattcata tcctcttcca ttagttcgat tccaacttct gtatcatggt
     3661 actcaaaagt gaaattagta tagatagtag tgattttcgt aagatcttgc atcgtcatcc
     3721 ctaaataaaa cggcgcgata tcagcaggcc ctgtttgcag tactttaaca gttgtgactc
     3781 tgctgtcttg gatgttcgct tcaaatgtga gatgactagc tttattttca taatatcttg
     3841 tttcaaaacc aaatccgcta gttaatcgat cataggcttg gccaaaatcc ttttccagct
     3901 gatcgatcgg ttttccgata taagtagaaa aaccagttgc tttaagctcc tggtatttcc
     3961 acgttgtcat ttggtttgat atttgttgat cagagttttc cttcacttca agttttggcg
     4021 gaaacaagac tggttccatg tagaatgcca caataactaa tagcagaatc agccaaaaca
     4081 aacccagtct tttcatgttt ccccaccttt cgcttattat ttctcagtca cttcaatcaa
     4141 aaggtcacca gacgaaatag tgtctcctga agacacatat acatgtgcga cttctccgtc
     4201 aaaacgtgct tcgatggccg tttccatttt catcgcttca gtaatcatca acgtatcacc
     4261 ttttttaacg ttatcgcctt ttttcactaa tacatccaaa acagaaccag acatcgttgc
     4321 tccaatttgt tctcggtttg tcggttcagc tttcaatcgg gtttggaccg tactgatgat
     4381 cgaattatct ttgatagtga tttcacgtct ttgtccattc aaattgaaga acagtacgcg
     4441 attcccttcg atatcgggtt ctccgatttc atctaaacga atgataagcg tttttccgcg
     4501 ttcgatttgt acattgattg tttcacccaa acgtatacct tggaaaaatg ttggtgtgtc
     4561 cagtaatgta acatcgccaa attgattata tgctttttga tagtccaaaa agacttgcgg
     4621 atacatcaaa tagcttaata cttcgtcttg ttttggttca tagccaattt tttctgccaa
     4681 ttcttttttt acttcgttga agtcgactga ttcagccaaa agacctggac gttcattcaa
     4741 tgcaggtctg cctttaagaa taatcttttg caatttttta ggaaatccgc caacgggttg
     4801 ccctagttct ccttggaaga aagtcacaac agattcaggg aaacttagtg tttcgccttt
     4861 ttcataaata tcttcttctg taagatcatt ttggaccata aatagtgcca tatctccaac
     4921 tactttagat gatggagtta ctttaacgat atcgccaaac atcaagttga cagtatgata
     4981 catttgtttg atttcatccc agcgatgacc taatccgaca gcttttgcct gttgctgtag
     5041 attcgaatac tgtcctcctg gcatttcatg catatatact tctgtttcag gagcattcaa
     5101 gccgttttca aacggtttgt aatacatgcg cacatcttcc caataatggt ttaatttctg
     5161 tgcattttca atcgtgattt ctggcagccg tggaccattg accagtgcat aatacaagct
     5221 gctcatgcta ggctgactag tattcccact cattgcactc attgccacgt caacgatgtc
     5281 tactcctgct tttgaagcag cagaataagt gatgattcca ttcccgctgg tatcatgagt
     5341 atgcagatgg attggcagat ctgtcgtttc tttcaattca ctaatcaaac gataagctgc
     5401 ctgcggttta agcagcccag ccatatcttt gattgcaatg atatgcgcac ccagttgttc
     5461 tagttccttt gccatgtctt tataatactg aacattgtat tttgcccgac ttggatcgtt
     5521 gatatcgcca gtatagcaaa tagctgcctc tgcgatttta ccagtatccc gtacagcttg
     5581 gatacttttt tccatttgtg gagtccagtt caaactatca aagattcgaa atacatcgat
     5641 tccttgcgct gctgcttctt tgacaaattc caccaataca ttgtcaggat aattagaata
     5701 cccaacggca tttgaacctc tgaaaagcat ttgcagcaac gtattaggca ttaaagaacg
     5761 aatttttctc aaccgttgcc aaggatcttc attcaaaaag cggtaagcca catcaaatgt
     5821 tgctcccccc cacatctcac ttgaaaagag ttcaggaaga ccttctccag tcaaacgtgc
     5881 aatctgcttg aaatcctttg tccgaacacg agttgctagc agactttgat gggcgtctct
     5941 aaatgtcgta tccgtcaaca acaaattttc ctgtcctttg atccattcga ccaaagcatc
     6001 ggcacccttc gcatctaata cattcttagc cgttacgtaa tcagttcttg tgattagatc
     6061 tttaggcata cgtggttctt cataaaatgg tttttcgcca ctttcgatac ctggaaaacc
     6121 attcacagta atttcaccga tatatttcat cgttttgttt ccgcggtctc gtagtcgagg
     6181 aaattcaaat aatgttgctg tactatcaat gaaggttgtt tttgcatttc ctgattggaa
     6241 ttcaggatgt gtgatgacat tgagcataaa tggaatgttt gttttcactc cacggattct
     6301 aaattctttc aagcaacgtt ccattttctg aatggctgtt tcaaaggtag caccatgcgt
     6361 acatactttt actagcaatg aatcaaaata cggtgtcact acatagcccg cgtaagcatt
     6421 tccgacatcc agacgtacgc caaaaccacc aggtgaacga tacgtatcga ttttccctgt
     6481 gtctggtaaa aagttattca acgggtcttc tgtagtgatt cggcattgaa tggctgaacc
     6541 atttaattta attccttctt gctgaggcaa tccgatttct ttatgcagat ccaaaccttg
     6601 agcaatcaac agttgcgttg ttacgatatc aatatctgta atcatttctg tgatcgtatg
     6661 ttccacttgg acgcgagggt tgacttcgat aaagtaaaaa tcatctcctt caacaagaaa
     6721 ttctactgta cctgcgttga catacccgac atatttcatc aattggacag ctgcctggca
     6781 gattttttga cgttgctgtt cattcataga gacacaaggc gctacttcta ctactttttg
     6841 gtgacggcgc tgaacagaac aatctctttc aaaaagatgg atgacgtttc catgcgtatc
     6901 cccgagaatc tgtacttcga tatgcttagg attagcgatg tatttttcaa catacacttc
     6961 atcactacca aaggctgctt ttgcttcgct tttcgcacgt tcgtagccct ctcttgcact
     7021 cttttcatcg tgagctacac gcatgccgcg tccgccgccg cctagagctg ccttgatcat
     7081 aatcggataa ccgtattgtt tcgcaaactc caaggcatcg tctatagaag cgattggtcc
     7141 atctgtgcct ggaatcgaag caatccccgc ttcgatagct gcagcttttg ctttgatttt
     7201 atctccaaaa atatctaaat gatgcagttt gggtccaaca aaaatgatgc cttcttcttc
     7261 gcaccgttgc gcaaaattca agttttccga caaaagaccg taacccggat gaatagcgtc
     7321 agctccgcat tctttcgcga tagaaatgat cccttcaata tctagatacg catcaatcgg
     7381 tttttttcct tgtcctacta aatatgcttc atccgctttg aaacggtgta cggaatattc
     7441 atcttcttct gcatagattc caacagtttt aattcctaat tctgtacaag cacgaaatac
     7501 acgtacagcg atttcacccc tgttagcaac taaaactttt ttcatcgaac cactcctaca
     7561 tcttttttta ttataaacct aagctcttgc gtttttcatc cgcactgatg ttgaggacaa
     7621 agccgacaca aatcgaaagc atcagcaaac tggagcctcc ttgacttaaa aatgggaacg
     7681 tgatccctgt caaaggaata acgccagtaa ttcctccaag attgacaaat acttggatca
     7741 aaaacattgc gccgatccca atgcacaaaa gcgaattgaa cggatctttt gaacggatac
     7801 ctacaaggac aatgcgcaca atcataaaga atagcaacgc taatatcaaa atccccataa
     7861 tcactcccag ttcttccact acgattgcat aaataaaatc ggtttgtgct tcttgtaaaa
     7921 atcctttttt ctggatactg ttacctaatc ccctgccaaa tagtcctccg ttgaacatgg
     7981 catagtagcc gttgactagt tgatgccctt tatttaattc gtctgagaaa ggattttggt
     8041 aaatagcaaa tcgattatag atatactgta aacgtccggg taagatcttt ccgtttgtga
     8101 tattgatcag ccagatcgta aaagtactta gacaaaagcc tcctactcct acaatcaaag
     8161 tatacacata attgactccg ctggctaaaa gcagaaccaa aataatcaaa aagataactg
     8221 ccgcattacc aaaatcggga agaatagcga ctaatgcagt aagtccgata accattgcaa
     8281 gtggacgttt cacagtccca agaaaatctt tttgtacact gttttgtctt ttagataaag
     8341 tcagagacag gtaccagata gaaatgattt ttaggtattc ggcaggttgg atcgagaatc
     8401 ctgcaatttg cagccaacct ttcgccccat tgatctcctt accaaaaaac acaacaatca
     8461 gcagcaaaat cgtcaaaact gcgatggctg ccatgatcaa acgctggttt ttcaaaacat
     8521 ctgttttcat tttataaagt agtgcgatag caatcaagct aagcacccaa aaaatactct
     8581 ggttaatgac tgatgcggac ggattttgtc cgttttctaa taatagatac gacgtagaac
     8641 tatataccat gatcaatccc aatacacata aaattaaata gggaatcaag atgctatagt
     8701 ctaatagatg cctttttttc actttcttag gcaagtctat tcctccttgc caacttcttt
     8761 tttatcataa atttcttggt aaacctcact atataactga ttcaattctc gttctagctc
     8821 acttaacatc agctggccct cgttctttcc gattactcct atacggacag caaaaccgac
     8881 ttgccgggaa aagccgtaca tctgtgtatc taccacttct tcgaaggctt tgcattgtga
     8941 gatacagaga ctgttgtttt gatttcggat taatcgtttg atccgttctg cttcatcatt
     9001 taatacttgg atcgcaagtt ttttcgacaa agaatccatc tgtgcttacc tcccattcta
     9061 attccttaat tataacatat ttgattttca taaggagcaa aaaaaacaga tttaaccagt
     9121 tgtttttatt catttttacg tattcgttct ttctacttat ccagaacgtt agagtccgca
     9181 ttttttatcc gagataaaaa ggcttcgcca tatttatcaa gcttgctttg gccaactcct
     9241 tttatctgaa gcatctccaa tctgcttgat ggacgtattc ggctcatttc tttcaatgtg
     9301 gaatctgaaa atacgacata cgggggaacg cctgcttctg atgctagatc cgttcgcagt
     9361 tcccgcagca cttcaaatag tgtatcggtt tcttcatccg atagctgctg gactttcttg
     9421 ggttcctttc gataaacaga ttcttgtcct ttcaatactt gtatccctct ttctgttact
     9481 ttcaaaacag gatactcacc attgatggtc agaagatagc cattcgatat caaatattca
     9541 attaactgca tcgtttcttt ttgcgattgg tttttcatca gtccataggt agataaatgt
     9601 ccaaaaccta aagcttgcag cttttgttct ttcgatcctg ctaagacttt catcagcatt
     9661 tgtttaccat agttctcacc catacgtttt aagcaagaaa gaaccatctg tgcttctgtc
     9721 gttacttcta ctatttctct gtcatctaaa cagttaccac aacggttgca cggactgctt
     9781 gtctcgccaa aatagtttag gatgtattgc tgaagacagg actcaatata cacgtattct
     9841 gtcatcgctt ttaatttttc atattctttt tgcttttgcc cttcttctct ttgggactgc
     9901 tggataaaaa acttttggac ttggatatct tgaggagaga aaagcaggat ggcttcactg
     9961 gaaagaccat ctcttcccgc ccgaccagct tcctgatagt atgcttctag agagccagga
    10021 atttgataat ggataacaaa acggacattg cttttattta tacccatacc aaatgcattt
    10081 gtcgctacaa tcagctgcaa gcgatcgtat aaaaaggctt cttgcatttc tgttcgctca
    10141 ttctcattta gaccaccatg atatctaccc acggaaaaat cgaatttttt aagcaaatga
    10201 tagagacggt ctacttcttt tctcgttgct gcataaatga taccagattg atttttgttg
    10261 atctttaaat attcgattag atattgttct tttttttgat ctttaattac ttgaaatcgt
    10321 agattttctc gttcaaaacc agtctgaata tggttgtttt ctggaatttt taaaagtctt
    10381 tttatgtcag cagctacttg cacagtcgct gtagctgtca aagcaacaat tacaggacga
    10441 tttggcagtt gatctagctg gttcgccatc tgtaaataac ttgggcggaa atcatgcccc
    10501 cactgagaga tacaatgtgc ctcgtcaata gcgatcatac ggacgttcca tcgcttcatt
    10561 gcttggataa aatccggcat gataaaacgt tcaggagcga tataaagtaa acggtactgc
    10621 tgtctatcga tttcttggaa gcgtctagct gtttcgtaac catctaacga gctattgata
    10681 aaagtggcgg gtatacctaa ttgattcgcc gcgtccacct gatccttcat caaagagatt
    10741 aaaggggaca caaccacagt aagcccatct aatagtaaag ctggaagctg gtaacagatt
    10801 gatttcccac ttccggtagg catgattcct aatacatttt cctgatcaat gactttctgg
    10861 ataatttctt tttgaccagg tctaaactca tcataaccaa agtattgttt taataagtcg
    10921 ttcatcttcc cgctcctttc tcttgccatt ttacttcgat tttcttcgaa ggacaagttg
    10981 atctcctttc gtgaaacatt atcttattat
//
LOCUS       DASJOX010000081        10922 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJOX010000081 DASJOX010000000
VERSION     DASJOX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.082"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..281)
                     /locus_tag="U4A12_001996"
     CDS             complement(<1..281)
                     /locus_tag="U4A12_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774348.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="U4A12_001997"
     CDS             423..1541
                     /locus_tag="U4A12_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1774349.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="U4A12_001998"
     CDS             1669..3045
                     /locus_tag="U4A12_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1774350.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="U4A12_001999"
     CDS             3446..5026
                     /locus_tag="U4A12_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN1774351.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="U4A12_002000"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="U4A12_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="U4A12_002001"
     CDS             complement(5652..5972)
                     /locus_tag="U4A12_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1774352.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="U4A12_002002"
     CDS             complement(6120..8354)
                     /locus_tag="U4A12_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1774353.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="U4A12_002003"
     CDS             8833..9099
                     /locus_tag="U4A12_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN1774354.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="U4A12_002004"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="U4A12_002004"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN1774355.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DASJOX010000082        10864 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJOX010000082 DASJOX010000000
VERSION     DASJOX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.083"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            151..1410
                     /gene="lysA"
                     /locus_tag="U4A12_002005"
     CDS             151..1410
                     /gene="lysA"
                     /locus_tag="U4A12_002005"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN1774356.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1397..2197
                     /gene="dapF"
                     /locus_tag="U4A12_002006"
     CDS             1397..2197
                     /gene="dapF"
                     /locus_tag="U4A12_002006"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN1774357.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2200..3081
                     /locus_tag="U4A12_002007"
     CDS             2200..3081
                     /locus_tag="U4A12_002007"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN1774358.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3078..3794
                     /locus_tag="U4A12_002008"
     CDS             3078..3794
                     /locus_tag="U4A12_002008"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN1774359.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3800..4495
                     /gene="dapD"
                     /locus_tag="U4A12_002009"
     CDS             3800..4495
                     /gene="dapD"
                     /locus_tag="U4A12_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN1774360.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4511..5512
                     /locus_tag="U4A12_002010"
     CDS             4511..5512
                     /locus_tag="U4A12_002010"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN1774361.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5509..6870
                     /locus_tag="U4A12_002011"
     CDS             5509..6870
                     /locus_tag="U4A12_002011"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN1774362.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6830..7981
                     /locus_tag="U4A12_002012"
     CDS             6830..7981
                     /locus_tag="U4A12_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN1774363.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7956..9125
                     /locus_tag="U4A12_002013"
     CDS             7956..9125
                     /locus_tag="U4A12_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1774364.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9300..10679
                     /locus_tag="U4A12_002014"
     CDS             9300..10679
                     /locus_tag="U4A12_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1774365.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3609 a         1904 c         2428 g         2923 t
ORIGIN      
        1 aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa
       61 ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa
      121 cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca
      181 gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg
      241 agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg
      301 ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga
      361 ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct
      421 agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt
      481 gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa
      541 ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa
      601 gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat
      661 gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta
      721 gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa
      781 atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc
      841 ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta
      901 aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa
      961 ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt
     1021 ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat
     1081 aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg
     1141 gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta
     1201 attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg
     1261 ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt
     1321 tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta
     1381 aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt
     1441 attagattac gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga
     1501 aaaatatgat acagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta
     1561 caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata
     1621 cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac
     1681 aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc
     1741 agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat
     1801 agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcatacagt
     1861 tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca
     1921 tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga
     1981 attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg
     2041 ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt
     2101 tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc
     2161 agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg
     2221 gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg
     2281 atccaattcc atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa
     2341 tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg
     2401 gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa
     2461 aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac
     2521 aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg
     2581 attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta
     2641 ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg
     2701 gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat
     2761 gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg
     2821 aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag
     2881 caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc
     2941 aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc
     3001 ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa
     3061 gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag
     3121 tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag
     3181 aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag
     3241 ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca
     3301 cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg
     3361 tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag
     3421 cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa
     3481 aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc
     3541 tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca
     3601 gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa
     3661 tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag
     3721 cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg
     3781 acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc
     3841 taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag
     3901 taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc
     3961 ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata
     4021 cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat
     4081 tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa
     4141 tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg
     4201 agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga
     4261 accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt
     4321 gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt
     4381 agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag
     4441 tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag
     4501 atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca
     4561 aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc
     4621 ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga
     4681 attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt
     4741 atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc
     4801 agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta
     4861 tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct
     4921 taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt
     4981 ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc
     5041 agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga
     5101 aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca
     5161 gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt
     5221 ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag
     5281 ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat
     5341 tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga
     5401 gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc
     5461 cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg
     5521 ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc
     5581 cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa
     5641 cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag
     5701 catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga
     5761 gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg
     5821 gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct gactgctcaa
     5881 ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac
     5941 tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga
     6001 tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta
     6061 ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa
     6121 agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat
     6181 catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg
     6241 gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa
     6301 ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa
     6361 aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc
     6421 aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa
     6481 catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca
     6541 gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc
     6601 gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat
     6661 aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc
     6721 acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat
     6781 caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga
     6841 atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa
     6901 atatttatat gaacaagtaa aaaacagcgg ggccgttatc catgaaatcg gcgaaacagg
     6961 actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga
     7021 tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat
     7081 gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac
     7141 taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat
     7201 ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc
     7261 aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg
     7321 acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat
     7381 tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt
     7441 ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat
     7501 ccaaggtgga acgatccgca atgttttagc tggagaagtt attgtatccg gaagtatccg
     7561 ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca
     7621 gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa
     7681 ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc
     7741 agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt
     7801 tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt
     7861 tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa
     7921 aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta
     7981 gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt
     8041 cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg
     8101 tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag
     8161 gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt
     8221 atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt
     8281 tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat
     8341 atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag
     8401 ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca
     8461 aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat
     8521 tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat
     8581 atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa
     8641 caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta
     8701 tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg
     8761 ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata
     8821 gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc
     8881 ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag
     8941 ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg
     9001 cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg
     9061 ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac
     9121 agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga
     9181 gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt
     9241 aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa
     9301 tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg
     9361 aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata
     9421 tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg
     9481 atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc
     9541 acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata
     9601 acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca
     9661 atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg
     9721 caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg
     9781 cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat
     9841 tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt
     9901 tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt
     9961 ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc
    10021 tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact
    10081 tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt
    10141 atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg
    10201 ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag
    10261 taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc
    10321 attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag
    10381 tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa
    10441 aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat
    10501 tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac
    10561 tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat
    10621 ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag
    10681 aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca
    10741 gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    10801 gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc
    10861 caaa
//
LOCUS       DASJOX010000083        10817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJOX010000083 DASJOX010000000
VERSION     DASJOX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.084"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..162)
                     /locus_tag="U4A12_002015"
     CDS             complement(25..162)
                     /locus_tag="U4A12_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1774366.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="U4A12_002016"
     CDS             complement(159..1343)
                     /locus_tag="U4A12_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1774367.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="U4A12_002017"
     CDS             complement(1599..1853)
                     /locus_tag="U4A12_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774368.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="U4A12_002018"
     CDS             complement(1878..3020)
                     /locus_tag="U4A12_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN1774369.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="U4A12_002019"
     CDS             complement(3080..4432)
                     /locus_tag="U4A12_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1774370.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="U4A12_002020"
     CDS             complement(4503..4847)
                     /locus_tag="U4A12_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1774371.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="U4A12_002021"
     CDS             complement(4857..5231)
                     /locus_tag="U4A12_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1774372.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="U4A12_002022"
     CDS             complement(5244..5558)
                     /locus_tag="U4A12_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1774373.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="U4A12_002023"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="U4A12_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="U4A12_002024"
     CDS             complement(8976..9638)
                     /locus_tag="U4A12_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774374.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="U4A12_002025"
     CDS             10255..10485
                     /locus_tag="U4A12_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774375.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="U4A12_002026"
     CDS             10491..10790
                     /locus_tag="U4A12_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774376.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DASJOX010000084        10610 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJOX010000084 DASJOX010000000
VERSION     DASJOX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.085"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..180
                     /locus_tag="U4A12_002027"
     CDS             <1..180
                     /locus_tag="U4A12_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEN1774377.1"
                     /translation="YEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLS
                     LIMIRSNNSTQILTN"
     gene            complement(331..1310)
                     /locus_tag="U4A12_002028"
                     /pseudo
     CDS             complement(331..1310)
                     /locus_tag="U4A12_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1315..2082)
                     /locus_tag="U4A12_002029"
     CDS             complement(1315..2082)
                     /locus_tag="U4A12_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1774378.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            2572..2997
                     /gene="lacA"
                     /locus_tag="U4A12_002030"
     CDS             2572..2997
                     /gene="lacA"
                     /locus_tag="U4A12_002030"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEN1774379.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            3014..3529
                     /gene="lacB"
                     /locus_tag="U4A12_002031"
     CDS             3014..3529
                     /gene="lacB"
                     /locus_tag="U4A12_002031"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEN1774380.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            3540..4472
                     /gene="lacC"
                     /locus_tag="U4A12_002032"
     CDS             3540..4472
                     /gene="lacC"
                     /locus_tag="U4A12_002032"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEN1774381.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            4475..5455
                     /gene="lacD"
                     /locus_tag="U4A12_002033"
     CDS             4475..5455
                     /gene="lacD"
                     /locus_tag="U4A12_002033"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1774382.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            5483..5800
                     /locus_tag="U4A12_002034"
     CDS             5483..5800
                     /locus_tag="U4A12_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1774383.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5800..7506
                     /locus_tag="U4A12_002035"
     CDS             5800..7506
                     /locus_tag="U4A12_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1774384.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7648..9054
                     /gene="lacG"
                     /locus_tag="U4A12_002036"
     CDS             7648..9054
                     /gene="lacG"
                     /locus_tag="U4A12_002036"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1774385.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            9077..9349
                     /locus_tag="U4A12_002037"
     CDS             9077..9349
                     /locus_tag="U4A12_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEN1774386.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            9370..10269
                     /locus_tag="U4A12_002038"
     CDS             9370..10269
                     /locus_tag="U4A12_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1774387.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3356 a         1781 c         2108 g         3365 t
ORIGIN      
        1 tatgaagcaa aattattgag ttatccaaga acagatacac catttattac agagaacgaa
       61 tttgcttatt taaaagcgaa ttttggcaaa tatagcggtt ttttaggact tgatcttgaa
      121 atgttgttat cattgataat gatacgttca aacaacagca cccaaatttt gacgaattag
      181 tgaaactcta tgaaaaagac gtagtaaaac atgcaacatc ctattctaat cagctggtaa
      241 agcttaatta ggttatataa gcttgaaaat aaagggtttg tccaatatgg acaagccttt
      301 ttcttatgtt ttgccacaga tttgccacac ttattgtgta agagtatcaa ggataggtat
      361 aatttgatac tttgtttttt tagtgatgtg caaataaatt tctgttacac gactacctct
      421 agaatgccct acacggtctt gaattgcttc taggggtatt ccttactctg ctaagaaact
      481 aatatgcgta tgacggaata tatgggtcgt aatatgttta ttaaaactaa gtttattatc
      541 aatatcaatt cctgctcgtt ttggattacc ttttcgattt aattttccat tttcaggtga
      601 gttgaggaaa ttgttaagga cactataatg aagaggtgtt ccattatagt cgggtttatg
      661 acgacttttg gaagtaaaaa tccatccatt atctttataa tcaggattga atctcatatg
      721 ttggtgattt aactcaattt cctctttaag aatttctata gcccttttag ataattggac
      781 atttcggtaa ctttgtaagt tttttacatt accttcatag ctattttctg cagctcctcc
      841 gtgagtggac tgactaatga tgatattttg cttttcaaaa tattgatagg caccaatatt
      901 ttggagtttc gactctgtaa gtcaacaggg ccattagtta gaatacctat tgggatgcgg
      961 tttttatgta gagccagcag tagttgatgt atctcaggaa tcatcgtaat ctgatcgagt
     1021 tcatattcat agtcagcttg gaagtctaga gcttctgtcc tagaaatact atcgatgcca
     1081 aattcttcca tagtcttctg acagcgaaaa attctaagct gctcaatact gatgagtttt
     1141 ttcgtgtatt taggaaaagc aacatcagac caatatctga aacgtttgta aatgttatct
     1201 atttgttgga tatcaatcgt tggaaaacat ttctcaacgc tacgtttaaa ggggagttga
     1261 tggtcataaa gagtgtcatc aaggtcaaaa acaatatttt tataagtcat aattctagtc
     1321 cgcttttaaa atagtagtgt actggctaaa ttcctctaag tgttgagggc taatctggtt
     1381 atctgtaatg atggtatcga gttgatctag attatagaag ttaaagaaat cgtatcgatc
     1441 gaatttagta ctgtctacta ataagaattt ttctacagca ttgtttaggg caagttgttg
     1501 aatcacacct tccttgtcac tatatgtagc aatagaatta tgggtaacag catttgcact
     1561 aacaaaagct ttggcaaatc taattgcttt taaatttgtc gaagccattg aacctacaaa
     1621 agctccagtt atttctctat attcaccgcc aagaagcaat aaatcaatgg tttcgctatc
     1681 atttagaatc aagaacacag ggagactatt tgtaatgaca cgaattttat aacctttacg
     1741 acctttcaat tctaatgcca gttgttcaag tgtagttcca ggtccaataa aaatagtatc
     1801 gccatctttg attaaagagc aggctttttt agcaatgtca attttttctt ttgtatttag
     1861 acttttcttc tcgatatgtg tcttttcaag tggcttttta gaggaaagca attgtgcacc
     1921 accatgagta cgtgttaaaa gtccatcagc ttctagagca tcaaggtctc tacgggctgt
     1981 catatcggaa atatctagtt catctattat ttcttttatg gttatggtcc catcaatctt
     2041 taacatatct aaaatttttt ctaatcgtcg ttttttgttc atatgaagac ccttctttca
     2101 taaagtaatt tttttccaaa gataattctc ttttaattgt atcataaaag acaatatttt
     2161 caaggtaaaa caaacaattt caaacaaaaa caaatgttag atgatgaaat aagaacagag
     2221 gattgacgta tattagctta ggtcagattt tgtataagac gaaaataaag taggacctct
     2281 taatcagtaa gttatagaaa gtaaaagact tttgtaatac ttgaatagat atttcacgtc
     2341 cattttgtga tggattaaat gaacaaaaat gaacaataat ttaacggtgt tatctatttt
     2401 ttaaaaaaac aaataaaaaa aacaaaaaat aaacaaaaaa agtgttgctt tttgtttgaa
     2461 tgcttgatat tatataaaca aagaaatgat gaaaacgttt tttcgaacat tttgcaaaat
     2521 gttttctact tctacgtagc atttctttct aaaatttagg aggtggtcaa aatggctatt
     2581 gttgttggtg cagatctcaa aggtacgaga ttaaaagatg ttgtgaaaaa ttttcttgta
     2641 gaagaaggtt ttgaggtaat tgatgtgact aaggacggac aagattttgt tgatgttacc
     2701 ttggcagttg cctctgaagt aaataaagat gagcaaaact taggtattgt aattgatgct
     2761 tatggagctg gtccattcat ggtagcaact aaaattaaag gtatggttgc agcagaagtt
     2821 tcagatgaac gctcagctta tatgacacgt ggacataaca attcacgaat gattacggtt
     2881 ggcgctgaaa tcgtcggtga tgagcttgct aaaaacatcg ctaaaggttt cgtcaatggt
     2941 aaatatgatg gcggacgtca tcaaatccga gtagatatgc ttaacaaaat gtgttaagaa
     3001 aaggagttaa ggaatgagaa tcgcaattgg atgtgaccac attgtcacag atgaaaaaat
     3061 ggcagtatca gaattcttga aatcaaaagg acatgaggtc cttgatttcg ggacatatga
     3121 ccacgcacgt actcactacc ctatctatgg taaaaaagtc ggtgaagcag tagtaagtgg
     3181 tcaagcagac ttgggagtat gtatctgtgg tacaggtgtc ggcattaaca atgcagtcaa
     3241 taaagttcca ggtgttcgct cagcgttggt tcgtgatatg acatcagcta tatatgcaaa
     3301 agaagagtta aatgcgaatg ttatcggttt cggtggaaag attactggtg gtcttcttat
     3361 gaatgacatc attgaagctt tcattgaagc tgagtacaaa ccaacagaag aaaataaaaa
     3421 attgattgca aaaatcgaac acgttgaaac acataatgca catcaagcag atgaagattt
     3481 ctttacagaa ttccttgaaa gatgggaccg tggtgactac cacgattaag gtataacaaa
     3541 tgattctgac agtcacacta aatccttcag tagacatctc ttatcctcta gaaacattga
     3601 aaattgatac agtgaatagg gtgaaagatg ttagtaagac agctggtgga aaaggcttaa
     3661 atgttactag agtcttgtat gaatctggtg ataaagttac tgctacaggt ttcttaggtg
     3721 gtaaaattgg tgaatttatt gaaagcgaat tagaacaatc tcctgttagt ccagcttttt
     3781 ataaaatctc aggaaacact agaaactgta ttgctatctt acacgagggt aaccaaacag
     3841 aaattttgga acaaggacca acaatttctc atgaagaagc agaaggcttt cttgaccact
     3901 atagcaatct tattaaacaa tcagaagttg tgacaatttc aggaagttta ccttcaggac
     3961 ttcccaacga ttattatgaa aaacttatcc aacttgcttc ggatgaagga gtagcagtcg
     4021 ttttggattg ttcaggtgca cctcttgaaa cagttctaaa atcgtcggct aaacctactg
     4081 ccatcaaacc taataatgag gaactttcac agttgcttgg aaaagaagta acaaaagata
     4141 ttgaagaact caaagacgtt cttaaagagt cactttttag tggtatcgaa tggatagtgg
     4201 tctcattagg tcgaaacggt gcctttgcaa aacatggtga tgttttctat aaggtagata
     4261 ttcctgatat cccagttgtt aatccagtcg gatcagggga ctcaacggta gctggtattg
     4321 catcagcttt aaatagtaaa aaaagtgacg ctgacttatt aaaacatgcg atgacattgg
     4381 gtatgttaaa tgctcaagaa acaatgacag ggcatgtgaa tatgactaat tacgaaacac
     4441 taaactcaca aattggagta aaagaggtat aaaaatggta cttacagaac agaaacgcaa
     4501 atctttggaa aaaatttcag ataaaaacgg tgttatctca gctttggcat ttgaccaacg
     4561 tggtgctttg aaacgtttga tggcacagta tcaagataca gaaccaactg tagctcaaat
     4621 ggaagaacta aaggttttgg ttgctgatga acttacaaaa tacgcttctt caatgttgct
     4681 tgaccctgaa tatggacttc cagcaacaaa agcattggat aaagaagcag gtcttcttct
     4741 tgcctatgaa aaaacaggtt atgatacatc aagtacaaaa cgtttgcctg actgcttgga
     4801 tgtttggtct gcaaaacgta tcaaagagca aggcgctgat gccgttaagt tcttgcttta
     4861 ctatgatgta gacagctcag atgaacttaa ccaacaaaaa caagcttata ttgaacgtgt
     4921 tggttctgaa tgtgtcgctg aagatattcc tttcttcttg gaaatccttg cttatgatga
     4981 agaaatctca gatgcaggtt ctgttgaata tgcaaaagtg aaacctcgca aagtcattga
     5041 agcgatgaaa gttttctctg atccacgctt taacattgat gttcttaaag tagaagtacc
     5101 agttaacgtt aaatatgtcg aaggttttgc tgatggtgaa gtagtttaca gtaaagctga
     5161 agctgctgac ttctttaaag cacaagaaga agcgactaac cttccataca tctacctaag
     5221 tgcaggtgta tctgctaaat tgttccaaga aacattgcaa tttgctcacg actcaggtgc
     5281 caagtttaat ggtgtgcttt gtggacgtgc tacttgggca ggatctgtag aaccttacat
     5341 caaagaaggt gaaaaagcag cacgtgaatg gttgcgtact actggattcg aaaatattga
     5401 tgaacttaac aaagttcttg ttaaaacagc tagtccatgg actgataaag tatagtaaaa
     5461 acataaaacg gaggatattg ttatgaacag agaagagatg actctcttag ggtttgaaat
     5521 tgttgcttat gctggagatg ctcgctctaa acttttagaa gcgcttaaag cggctgaaaa
     5581 tggtgatttc gctaaggcag atagtcttgt agtagaagca ggaagctgta ttgcagaggc
     5641 tcacaattct cagacagcta tgttggctcg agaagcttct ggggaggaac ttccatacag
     5701 cgttaccatg atgcatggtc aggaccactt aatgactact gtcttattaa aagatgtgat
     5761 tcatcacctc atcgaacttt ataaaagagg agcaaaataa tgcataaact cattgaactt
     5821 attgagaaag ggaaaccgtt ctttgagaaa atctctcgaa acatctatct tcgtgctatt
     5881 cgtgatggat ttatcgctgg tatgccagtc atccttttct catctatctt tatccttatt
     5941 gcctatgtcc caaatgcttg gggattccac tggtcaaaag acattgagac cttcttgatg
     6001 actccttata gctattcgat gggtatccta gcattctttg ttggtggtac taccgctaaa
     6061 gctttgacgg actcaatgaa ccgtgaccta cctgcgacca atcagattaa cttcttatct
     6121 acgatgctag cgtctatggt agggttcctt ttgatggcag ctgagcctgc aaaagaagga
     6181 ggcttcttga cagccttcac gggaacaaaa ggtcttttga cagcatttat tgcagccttt
     6241 gtgacagtta atgtttacaa ggtctgtgtg aaaaataatg ttactattcg tatgcctgaa
     6301 gaggttccac caaatatctc tcaagtattt aaagacttga ttccattcac tgtagcggtt
     6361 gttctccttt atggatttga actcattgtt aagggaactc ttggtgtaac cgttgcagaa
     6421 tcaatcggta cccttcttgc tcctctcttc tcagctgcag atggttacct tggaattaca
     6481 cttatctttg gtgcttatgc cttcttctgg tttgttggta tccacggtcc ttcaattgtc
     6541 gaaccagcaa tcgctgctat cacttatgcc aatatcgatg ccaacttgca tcttatccaa
     6601 gctggacaac atgcagacaa agttatcact tctggtactc aaatgtttat tgttaccatg
     6661 ggtggaacag gagctacatt gattgttcca ttcttgttca tgtggatttg taaatcagaa
     6721 cgtaaccgtg ccatcggacg tgcctcagtt gttccaacat tctttggggt taatgagcca
     6781 atcttatttg gtgcaccaat cgtattgaat ccgattttct ttgtaccgtt cattttcgct
     6841 ccgattgtca acgtttggat ctttaaattc tttgttgaca cattgaacat gaactcattc
     6901 tctgccaacc ttccatgggt tactcctggt ccgttgggaa ttgtactcgg tactaacttc
     6961 caagttctat catttatctt agccggactc ttggtagttg ttgatactat catttattac
     7021 ccatttgtta aggtatacga tgaacaaatt cttgaagaag aacgttctgg taaaacaaac
     7081 gatgctctta aagaaaaagt agctgcaaac ttcaatactg ctaaagcaga tgccgttctt
     7141 ggaaaagcag gcgttgctaa agaagatgta gcagcaaaca ataatatcac aaaagaaact
     7201 aatgtccttg ttctttgtgc aggtggaggt acaagtggct tgcttgccaa tgccttgaac
     7261 aaagcagcag cagaatacaa tgttccagtc aaagcggcag cgggtagtta cggtgctcac
     7321 cgtgaaatgt tgccagagtt tgacttggtt atcttggctc cacaggtcgc ttcaaacttc
     7381 gatgatatga aggctgaaac ggataaattg ggcattaaac ttgcaaaaac tgaaggagct
     7441 caatatatca aattgacacg tgatggacaa ggtgctcttg catttgttca acaacagttt
     7501 gattaataaa tttgtaaaag ttatttgaga cgagattatt ctttcaattc cttataatat
     7561 tgttaaccat atcattgcat ttcatttcct tggtttccgg aactcagaat atcgtctcaa
     7621 atgctttttt tgaaaggatt tatagttatg actaaaacac ttcctaaaga ttttattttt
     7681 ggtggtgcta cagctgctta tcaagcagaa ggtgcgactc acacggatgg taaaggccca
     7741 gttgcatggg ataagtatct tgaagacaat tattggtaca cagcagagcc tgcaagtgat
     7801 ttttaccata aatatcctgt tgatttagaa ttagctgaaa aatatggcgt taatggtatt
     7861 cgtatttcaa ttgcctggtc tcgtattttc ccaactggct atggcgaagt aaatgaaaaa
     7921 ggtgttgaat tctaccataa tctttttgcc gagtgccaca aacgtcatgt tgagccattc
     7981 gtgactttgc accactttga cacaccagaa gttcttcact cagatggaga tttcttaaac
     8041 cgcgaaaata tagaacactt tgtagattat gccgctttct gttttgaaga gttcccagaa
     8101 gtaaactact ggacaacttt caatgaaatt ggcccaattg gagatggtca atacttggtt
     8161 ggtaaattcc ctccaggcat tcaatatgat cttgccaaag tcttccaatc acaccacaat
     8221 atgatggttt ctcatgcacg tgctgtgaaa ttgtataaag ataaaggtta taaaggcgaa
     8281 attggtgtag tacatgcgtt gccaaccaaa tacccttatg atccagaaaa tccagcagat
     8341 gttcgtgccg ctgagttaga agatatcatc cataacaaat ttatcttgga tgcaacctac
     8401 cttggacact attcagataa aacgatggaa ggggtcaacc acatcctagc tgaaaatggt
     8461 ggagaacttg atcttcgtga tgaagacttc caagcacttg aagcagctaa agatttgaac
     8521 gatttccttg gtatcaacta ctacatgagt gattggatgc aagcctttga tggtgagact
     8581 gaaatcattc acaatggtaa gggtgaaaaa ggaagttcta agtaccagat taaaggtgtt
     8641 ggtcgccgaa tcgcaccaga ttatgtgcct agaacggatt gggactggat tatttatcct
     8701 gaaggcttgt atgaccaaat catgcgagtg aaaaatgatt atccaaacta caagaagatt
     8761 tacatcactg aaaacggtct tggatacaaa gatgagtttg tagataatac cgtttatgat
     8821 gatggtcgta ttgattacgt gaagaaacac ttggaagttt tatcagacgc aattgcggat
     8881 ggtgcaaatg ttaaaggtta cttcatctgg tcacttatgg atgtcttctc atggtcaaac
     8941 ggatacgaaa aacgttatgg actattctat gtagactttg acacgcaaga acgctatcct
     9001 aagaaatcag ctcattggta taagaaagta gcagaaactc aagtgataga gtaatattac
     9061 ttgaaaatgt aaaaaattgt tatccgatac tttagaaaaa gtacaacact tatttagacc
     9121 aggctttgaa aaggtatata ttgttagttt cgaagattgt cctatgattc ctgagcttga
     9181 agctaccccg ttgctaaaat gtggtaaatg gtatgtttca actggaaaag agtggatttg
     9241 ccattctgat ttagagttat cagcatttga atgggaattt ttacaatcac tcgatgtaga
     9301 aatacgtgag actattcact tcgaggtaaa ttatttgcct ttccaataat aaaaagaaag
     9361 gaattagttg tgactatcga attaaaaaac gagtatctta ctgttcagtt taaaacactt
     9421 ggtggtcaac tgacttcgat taaagacaag gatgggatag agtatctctg gcaagctgat
     9481 ccaaattatt ggaatggaca ggcgcctatt ctatttccta tctgtggtag tttacgaaat
     9541 gattgggcta tttatagacc tcaagaaaga ccctttttta caggtcttat tcgtagacat
     9601 ggttttgttc gaaaagaaga atttattctg gaagaagtca atgataatag cgtgaccttt
     9661 agtattaaac caaatgctga gatgcttgat aattacctat atcagtttga actgagagtt
     9721 gtttatacct tgaatggtaa atcaattaga accgaatttc aggtaacgaa cttggaaatg
     9781 gaaaaaacaa tgccttactt tattggagcc catccagcat tcaatagtcc tttagtagaa
     9841 ggtgaaaact atgaggatta ttatattgaa tttagtgagg ttgagtcttg ctcgatacca
     9901 aagagttttc ctgagacagg attattagat ttgcaagatc ggacaccttt tttggaaaat
     9961 caaaaaattc tcgatttgga ctactcactt ttttcccatg atgccattac acttgaccag
    10021 ctcaagtctc gtagcgtaac gcttcggtca aggaagtctg gtaaaggtct tcgagtagat
    10081 tttgatgatt ttccgaactt gattctgtgg tctacaacta ataaaagtcc attcattgct
    10141 ttggaacctt ggagtggtct ctccacttca cttgaagaag gtaatttctt agaagataaa
    10201 agacaggtaa cgaaaatttc acctcaagaa acttctagaa aaatctatga tattactatc
    10261 cttaattaaa atttgttatt atttgagtct tcaataagat ttgaatactc ttaccttgtg
    10321 atgatagatt ggtcttctcc gtgccagtct tacatcgcaa ggtttttaag ttttttgtgg
    10381 gtctatgtca actgtagtgg gtgacgaaaa gctaatatct aaagacagtc tggtagtaaa
    10441 aaataagatt atttactact tcttgattta aaataatagt ggagccatta atttgtggca
    10501 aatttaattt aatattttgc ctcaaattag ccacactttt tatagaacta agaggaaaaa
    10561 tgaaattttc ttcctattaa tatagttgtt tgaaatctta aatatagcgt
//
LOCUS       DASJOX010000085        10482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJOX010000085 DASJOX010000000
VERSION     DASJOX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.086"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..657
                     /locus_tag="U4A12_002039"
     CDS             34..657
                     /locus_tag="U4A12_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774388.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="U4A12_002040"
     CDS             1107..2156
                     /locus_tag="U4A12_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1774389.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="U4A12_002041"
     CDS             2153..3034
                     /locus_tag="U4A12_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774390.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="U4A12_002042"
     CDS             3037..3807
                     /locus_tag="U4A12_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1774391.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="U4A12_002043"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="U4A12_002043"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN1774392.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="U4A12_002044"
     CDS             5118..6281
                     /locus_tag="U4A12_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1774393.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="U4A12_002045"
     CDS             6398..6808
                     /locus_tag="U4A12_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1774394.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="U4A12_002046"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="U4A12_002046"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1774395.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="U4A12_002047"
     CDS             9381..9665
                     /locus_tag="U4A12_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774396.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="U4A12_002048"
     CDS             9825..10061
                     /locus_tag="U4A12_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1774397.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="U4A12_002049"
     CDS             10058..10456
                     /locus_tag="U4A12_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1774398.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3655 a         1763 c         2221 g         2843 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca at
//
LOCUS       DASJOX010000086        10235 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJOX010000086 DASJOX010000000
VERSION     DASJOX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.087"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..1537)
                     /locus_tag="U4A12_002050"
     CDS             complement(29..1537)
                     /locus_tag="U4A12_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN1774399.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1696..1887
                     /locus_tag="U4A12_002051"
     CDS             1696..1887
                     /locus_tag="U4A12_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774400.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2090..3007
                     /locus_tag="U4A12_002052"
     CDS             2090..3007
                     /locus_tag="U4A12_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN1774401.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3000..4073
                     /locus_tag="U4A12_002053"
     CDS             3000..4073
                     /locus_tag="U4A12_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774402.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4221..4739
                     /locus_tag="U4A12_002054"
     CDS             4221..4739
                     /locus_tag="U4A12_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774403.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4720..5112
                     /locus_tag="U4A12_002055"
     CDS             4720..5112
                     /locus_tag="U4A12_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN1774404.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5188..6123
                     /locus_tag="U4A12_002056"
     CDS             5188..6123
                     /locus_tag="U4A12_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN1774405.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6256..7035
                     /locus_tag="U4A12_002057"
     CDS             6256..7035
                     /locus_tag="U4A12_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1774406.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7028..7345
                     /locus_tag="U4A12_002058"
     CDS             7028..7345
                     /locus_tag="U4A12_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1774407.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7589..9199
                     /locus_tag="U4A12_002059"
     CDS             7589..9199
                     /locus_tag="U4A12_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1774408.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9356..10204
                     /locus_tag="U4A12_002060"
     CDS             9356..10204
                     /locus_tag="U4A12_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1774409.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3217 a         1795 c         2067 g         3156 t
ORIGIN      
        1 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
       61 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
      121 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
      181 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
      241 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
      301 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
      361 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
      421 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
      481 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
      541 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
      601 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
      661 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
      721 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
      781 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
      841 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
      901 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
      961 catgatacag ttggttcaag caagtagcga gctttccact tcctgggcca ggagctgtca
     1021 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
     1081 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
     1141 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
     1201 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
     1261 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
     1321 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
     1381 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactaac ttcccaccaa
     1441 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
     1501 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
     1561 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
     1621 aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga tagtttttga
     1681 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
     1741 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
     1801 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
     1861 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
     1921 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
     1981 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
     2041 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
     2101 agatacaaat gcaaattatg agccagacga actagaggag acgaacgcgg atcaggatac
     2161 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
     2221 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
     2281 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
     2341 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
     2401 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
     2461 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
     2521 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
     2581 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
     2641 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
     2701 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
     2761 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
     2821 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
     2881 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
     2941 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
     3001 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
     3061 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
     3121 ctatgctaga ggaaacaaaa atacatgaaa aagaaaaagt aactccgacg aatacgaata
     3181 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
     3241 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
     3301 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
     3361 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
     3421 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
     3481 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
     3541 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
     3601 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
     3661 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
     3721 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
     3781 tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg
     3841 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
     3901 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
     3961 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
     4021 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
     4081 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
     4141 agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg
     4201 aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg
     4261 ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg
     4321 ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc
     4381 tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga
     4441 tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa
     4501 ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta
     4561 tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag
     4621 gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc
     4681 cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt
     4741 ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa
     4801 tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa
     4861 gaaaatggtc aaaacttttc ggaaaaagaa gtggtcttca aaaaaggcga tgatttactg
     4921 agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa
     4981 gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt
     5041 gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg
     5101 tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag
     5161 ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc
     5221 gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca
     5281 atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat
     5341 cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa
     5401 tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc
     5461 ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa
     5521 gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt
     5581 tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg
     5641 cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt
     5701 gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt
     5761 gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt
     5821 ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta
     5881 tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca
     5941 gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa
     6001 gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct
     6061 gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa
     6121 taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata
     6181 aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt
     6241 aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca
     6301 gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa
     6361 aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc
     6421 gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg
     6481 atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg
     6541 aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat
     6601 gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat
     6661 gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc
     6721 tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc
     6781 atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta
     6841 ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc
     6901 attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa
     6961 ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc
     7021 aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata
     7081 tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag
     7141 tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt
     7201 cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt
     7261 agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc
     7321 tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca
     7381 taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt
     7441 ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta
     7501 ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt
     7561 ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg
     7621 gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct
     7681 aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta
     7741 tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa
     7801 aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag
     7861 tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc
     7921 tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag
     7981 aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca
     8041 aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt
     8101 gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga
     8161 agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat
     8221 gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg
     8281 ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt
     8341 tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc
     8401 actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg
     8461 tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc
     8521 tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa
     8581 aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat
     8641 gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg
     8701 atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac
     8761 gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat
     8821 cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt
     8881 atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga
     8941 ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac
     9001 cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat
     9061 ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt
     9121 atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca
     9181 tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac
     9241 tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac
     9301 ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa
     9361 acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga
     9421 cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga
     9481 tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc
     9541 acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga
     9601 cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc
     9661 gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat
     9721 gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga
     9781 tgaaatactt gcacatatcg atcgaatccg agataatacc tatgcttatc tgattgtaga
     9841 tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag
     9901 cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa
     9961 aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa
    10021 cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat
    10081 tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt
    10141 cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca
    10201 ataattcctt ttattcaaaa aacataccaa aaata
//
LOCUS       DASJOX010000087        10084 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJOX010000087 DASJOX010000000
VERSION     DASJOX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.088"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            162..392
                     /locus_tag="U4A12_002061"
     CDS             162..392
                     /locus_tag="U4A12_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN1774410.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="U4A12_002062"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="U4A12_002062"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1774411.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="U4A12_002063"
     CDS             1803..1979
                     /locus_tag="U4A12_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1774412.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="U4A12_002064"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="U4A12_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN1774413.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="U4A12_002065"
     CDS             2474..2941
                     /locus_tag="U4A12_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774414.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="U4A12_002066"
     CDS             3026..3910
                     /locus_tag="U4A12_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1774415.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="U4A12_002067"
     CDS             4024..4260
                     /locus_tag="U4A12_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN1774416.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="U4A12_002068"
     tRNA            4348..4428
                     /locus_tag="U4A12_002068"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="U4A12_002069"
     tRNA            4435..4506
                     /locus_tag="U4A12_002069"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="U4A12_002070"
     CDS             4631..4801
                     /locus_tag="U4A12_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774417.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="U4A12_002071"
     CDS             5021..5728
                     /locus_tag="U4A12_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1774418.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="U4A12_002072"
     CDS             complement(5788..6432)
                     /locus_tag="U4A12_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN1774419.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="U4A12_002073"
     CDS             6559..6846
                     /locus_tag="U4A12_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774420.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="U4A12_002074"
     CDS             6954..7274
                     /locus_tag="U4A12_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774421.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="U4A12_002075"
     CDS             7355..8668
                     /locus_tag="U4A12_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1774422.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="U4A12_002076"
     CDS             8665..9384
                     /locus_tag="U4A12_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN1774423.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="U4A12_002077"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="U4A12_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN1774424.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DASJOX010000088        10063 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJOX010000088 DASJOX010000000
VERSION     DASJOX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.089"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(526..750)
                     /locus_tag="U4A12_002078"
     CDS             complement(526..750)
                     /locus_tag="U4A12_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774425.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            941..3718
                     /locus_tag="U4A12_002079"
     CDS             941..3718
                     /locus_tag="U4A12_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1774426.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3799..4218
                     /locus_tag="U4A12_002080"
     CDS             3799..4218
                     /locus_tag="U4A12_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN1774427.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4230..4700
                     /locus_tag="U4A12_002081"
     CDS             4230..4700
                     /locus_tag="U4A12_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774428.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4717..5496
                     /locus_tag="U4A12_002082"
     CDS             4717..5496
                     /locus_tag="U4A12_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1774429.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5483..6304
                     /locus_tag="U4A12_002083"
     CDS             5483..6304
                     /locus_tag="U4A12_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1774430.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6321..7370
                     /locus_tag="U4A12_002084"
     CDS             6321..7370
                     /locus_tag="U4A12_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1774431.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7383..8387
                     /locus_tag="U4A12_002085"
     CDS             7383..8387
                     /locus_tag="U4A12_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1774432.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9020..10039
                     /locus_tag="U4A12_002086"
     CDS             9020..10039
                     /locus_tag="U4A12_002086"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1774433.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3375 a         1656 c         2098 g         2934 t
ORIGIN      
        1 aagtaaatgt ttatagatga aactctattt acttcttttt taatttgttc ttgcaatctt
       61 ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga ttttcaaaga
      121 aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc ttagaggaat
      181 aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga tggaacgaag
      241 acaacctccc gacatcaagt caaaagctac ttgagatttt gatctgaaaa taaaaaaaga
      301 aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg aaaaaactaa
      361 gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta ctctctgaaa
      421 gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca catataagga
      481 tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt ttgtatggaa
      541 ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt cggctatata
      601 ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa gcaaaggcaa
      661 aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc aggaagtatg
      721 tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt attctttcat
      781 atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa taaaaattat
      841 aagggataag taggctgctg tctataataa gatatatttt tcaattcttt attaaagatt
      901 ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt tgttgaagaa
      961 ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac atatgaacga
     1021 ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca cagtcagtca
     1081 ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc gaccagtgat
     1141 ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga atttttattt
     1201 ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag acttattctc
     1261 ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca aaacagctct
     1321 ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca caggtaaaag
     1381 ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag aagattctgc
     1441 accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt tgactagcaa
     1501 tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa aaggggcttt
     1561 tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa caccagaagg
     1621 tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg gagaacctaa
     1681 tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga ttactagtca
     1741 tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg cacttaatca
     1801 gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag aacaaagaaa
     1861 aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa gtcagaaatt
     1921 atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa aagcatatgc
     1981 tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa aagaactact
     2041 tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc aagaccagac
     2101 cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta aaagttttga
     2161 gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg aaggaaaatt
     2221 aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta gacaagaaaa
     2281 acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac aaatggaaac
     2341 tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact atctctatca
     2401 gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa tcaaagaatt
     2461 agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac gaattctgga
     2521 attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt tactgacgat
     2581 ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga tgattgctgc
     2641 tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat tacaagagga
     2701 tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg cttcagaaat
     2761 ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag tagacatggg
     2821 atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc ttttgatttt
     2881 gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa aaaatgtatc
     2941 attagaagag atagcagaag agactgttcg acagatccag ccagaatggc gactgctcta
     3001 tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa tcggtacggc
     3061 ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt tgaaaatcat
     3121 cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat tttccatata
     3181 cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg actatctttc
     3241 attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt tggacactac
     3301 tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt tttcattgga
     3361 aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag aaatcgatca
     3421 ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca aaaaattagc
     3481 gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc caatcgaaac
     3541 ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg ctattaaatc
     3601 tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg agattgccta
     3661 tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag aattttgaac
     3721 ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta tgtgtaagtg
     3781 aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg aaaatttgct
     3841 tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat tactgtattc
     3901 gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct ttttatgagg
     3961 tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt caacaacgaa
     4021 ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa tttgccacta
     4081 ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt gatccagcag
     4141 gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa tcagaaaata
     4201 gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt
     4261 tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac tgtattttga
     4321 ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa ttagccaagc
     4381 cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg
     4441 gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat gcatataagc
     4501 tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag gcaaaacctg
     4561 gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa cgcttgaaac
     4621 gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg
     4681 atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc agtcttatta
     4741 ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt gctttctcgt
     4801 ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt
     4861 atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc ttcgattcca
     4921 cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc tggagccggt
     4981 acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat attgaaaaat
     5041 atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta tgcagaagaa
     5101 ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct ttccttgatg
     5161 cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg aaatctatta
     5221 aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt gattccagct
     5281 cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc atattttttc
     5341 ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc tatttttggt
     5401 gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc acttcaaaca
     5461 actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca aaataacgaa
     5521 aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt ggaattatga
     5581 acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa agttatatca
     5641 gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta acacgacccc
     5701 acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa ataaggatga
     5761 tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg gacctcttgc
     5821 agggttagga gattctttct tttggggaac tttgcgattg attgccacag gtgtgggtac
     5881 ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga ttttcaatat
     5941 tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag gaacgggttt
     6001 tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg cgtctattat
     6061 tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata ttcctttaaa
     6121 aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata tcgttccaaa
     6181 tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag aagtgaaacc
     6241 attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga tagggatttt
     6301 ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa gaaaaggaag
     6361 tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg aaaaaaacag
     6421 tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca gcgaagccag
     6481 cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat tttttcaatt
     6541 atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc gggaaaagtg
     6601 cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt gttgctatca
     6661 caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat ttaggtgccg
     6721 gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta cagttactac
     6781 tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata gaacaagact
     6841 acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa aaaacggaag
     6901 aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc accattggat
     6961 atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa acaatccgcg
     7021 ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg gaggcagatg
     7081 cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt
     7141 tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca aaaaaagcaa
     7201 gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct gtattagcgc
     7261 ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt attgacttga
     7321 acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa gaggtggaaa
     7381 tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac
     7441 gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac gctgtctatt
     7501 atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat atgaatggga
     7561 aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact ggaaacaaaa
     7621 gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact caagaagtag
     7681 tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt atcgataaag
     7741 cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct cctggaacag
     7801 aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga gaattcgaag
     7861 cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta gtggaagcag
     7921 agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc
     7981 attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc atgtgttact
     8041 gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt cttcacggga
     8101 cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa gatgaacaga
     8161 gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat tatacattga
     8221 tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt
     8281 ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag cagttgaact
     8341 gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat agactggata
     8401 aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt tttaaaatat
     8461 tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa cttataatct
     8521 gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact atttagttgt
     8581 tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac atagcgtttt
     8641 gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag agacaatctg
     8701 tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt attttaaatc
     8761 aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt aacattgtca
     8821 aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa atgtagacta
     8881 ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac aaacacttaa
     8941 aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa ttatcaatta
     9001 tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg gatcttgggg
     9061 cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt ggggaaatgt
     9121 acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt tacctgattt
     9181 gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg ataacgcaga
     9241 tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg aacttattgg
     9301 taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac aagatacgca
     9361 taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac aggcaattgt
     9421 tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca caacaattac
     9481 agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca tgaatgatta
     9541 tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag cactgaaaaa
     9601 tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg atgcaaaagc
     9661 agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga tgggtgccaa
     9721 tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt gtacaagtgt
     9781 tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc tggaagaagt
     9841 tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag cagtagagct
     9901 agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg ttttgtataa
     9961 tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa aaatggagaa
    10021 tgaattttct cttcgttaaa tagatgatca atagttaatc gtc
//
LOCUS       DASJOX010000089         9919 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJOX010000089 DASJOX010000000
VERSION     DASJOX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.090"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(495..881)
                     /locus_tag="U4A12_002087"
     CDS             complement(495..881)
                     /locus_tag="U4A12_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1774434.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(878..1399)
                     /locus_tag="U4A12_002088"
     CDS             complement(878..1399)
                     /locus_tag="U4A12_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1774435.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(1401..2435)
                     /gene="sppA"
                     /locus_tag="U4A12_002089"
     CDS             complement(1401..2435)
                     /gene="sppA"
                     /locus_tag="U4A12_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1774436.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(2468..3883)
                     /locus_tag="U4A12_002090"
     CDS             complement(2468..3883)
                     /locus_tag="U4A12_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN1774437.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3852..6005)
                     /locus_tag="U4A12_002091"
     CDS             complement(3852..6005)
                     /locus_tag="U4A12_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN1774438.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6263..6499
                     /locus_tag="U4A12_002092"
     CDS             6263..6499
                     /locus_tag="U4A12_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN1774439.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6518..6733
                     /locus_tag="U4A12_002093"
     CDS             6518..6733
                     /locus_tag="U4A12_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN1774440.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(7436..8188)
                     /locus_tag="U4A12_002094"
     CDS             complement(7436..8188)
                     /locus_tag="U4A12_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1774441.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(8203..9450)
                     /locus_tag="U4A12_002095"
     CDS             complement(8203..9450)
                     /locus_tag="U4A12_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1774442.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(9526..9672)
                     /locus_tag="U4A12_002096"
     CDS             complement(9526..9672)
                     /locus_tag="U4A12_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN1774443.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(9776..>9919)
                     /locus_tag="U4A12_002097"
     CDS             complement(9776..>9919)
                     /locus_tag="U4A12_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1774444.1"
                     /translation="YLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDK
                     SQF"
BASE COUNT         3259 a         1742 c         1380 g         3538 t
ORIGIN      
        1 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
       61 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
      121 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
      181 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
      241 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
      301 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
      361 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
      421 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
      481 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
      541 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
      601 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
      661 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
      721 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
      781 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
      841 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
      901 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
      961 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
     1021 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
     1081 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
     1141 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
     1201 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
     1261 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
     1321 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
     1381 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
     1441 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
     1501 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
     1561 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
     1621 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
     1681 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
     1741 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
     1801 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
     1861 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
     1921 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
     1981 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
     2041 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
     2101 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
     2161 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
     2221 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
     2281 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
     2341 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
     2401 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
     2461 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
     2521 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
     2581 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
     2641 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
     2701 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
     2761 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
     2821 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
     2881 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
     2941 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
     3001 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
     3061 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
     3121 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
     3181 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
     3241 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
     3301 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
     3361 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
     3421 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
     3481 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
     3541 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
     3601 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
     3661 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
     3721 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
     3781 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
     3841 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
     3901 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
     3961 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
     4021 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
     4081 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
     4141 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
     4201 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
     4261 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
     4321 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
     4381 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
     4441 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
     4501 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
     4561 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
     4621 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
     4681 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
     4741 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
     4801 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
     4861 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
     4921 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
     4981 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
     5041 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
     5101 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
     5161 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
     5221 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
     5281 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
     5341 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
     5401 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
     5461 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
     5521 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
     5581 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
     5641 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
     5701 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
     5761 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
     5821 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
     5881 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
     5941 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
     6001 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
     6061 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
     6121 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
     6181 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
     6241 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
     6301 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
     6361 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
     6421 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
     6481 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
     6541 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
     6601 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
     6661 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
     6721 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
     6781 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
     6841 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
     6901 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
     6961 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
     7021 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
     7081 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
     7141 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
     7201 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
     7261 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
     7321 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
     7381 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
     7441 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
     7501 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
     7561 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
     7621 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
     7681 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
     7741 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
     7801 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
     7861 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
     7921 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
     7981 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
     8041 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
     8101 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
     8161 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
     8221 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
     8281 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
     8341 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
     8401 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
     8461 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
     8521 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
     8581 tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa
     8641 ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta attcttttat
     8701 ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa
     8761 atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt
     8821 atattttttt agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg
     8881 cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat
     8941 taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa
     9001 taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc
     9061 aacaatccta tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga
     9121 atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat
     9181 atacgtcata accatggaag taataataga tctgaaatca tcaataagca tacataatag
     9241 taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc
     9301 gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat
     9361 caataaacat acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa
     9421 gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt
     9481 aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt
     9541 aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc
     9601 agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt
     9661 tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat
     9721 atacctattt ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa
     9781 attgagattt atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa
     9841 tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat
     9901 aagcagtaaa atatagata
//
LOCUS       DASJOX010000090         9894 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJOX010000090 DASJOX010000000
VERSION     DASJOX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.091"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..684
                     /locus_tag="U4A12_002098"
     CDS             205..684
                     /locus_tag="U4A12_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774445.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="U4A12_002099"
     CDS             717..2945
                     /locus_tag="U4A12_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1774446.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="U4A12_002100"
     CDS             3074..4717
                     /locus_tag="U4A12_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1774447.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="U4A12_002101"
     CDS             complement(4790..5335)
                     /locus_tag="U4A12_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN1774448.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="U4A12_002102"
     CDS             complement(5358..5843)
                     /locus_tag="U4A12_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1774449.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="U4A12_002103"
     CDS             complement(5833..6645)
                     /locus_tag="U4A12_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN1774450.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="U4A12_002104"
     CDS             complement(6784..8316)
                     /locus_tag="U4A12_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1774451.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="U4A12_002105"
     CDS             complement(8425..9456)
                     /locus_tag="U4A12_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1774452.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3292 a         1834 c         1904 g         2864 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttga
//
LOCUS       DASJOX010000091         9875 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJOX010000091 DASJOX010000000
VERSION     DASJOX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.092"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..548)
                     /locus_tag="U4A12_002106"
     CDS             complement(36..548)
                     /locus_tag="U4A12_002106"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN1774453.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(562..2859)
                     /gene="recJ"
                     /locus_tag="U4A12_002107"
     CDS             complement(562..2859)
                     /gene="recJ"
                     /locus_tag="U4A12_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN1774454.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3001..3444)
                     /locus_tag="U4A12_002108"
     CDS             complement(3001..3444)
                     /locus_tag="U4A12_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN1774455.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3445..4233)
                     /locus_tag="U4A12_002109"
     CDS             complement(3445..4233)
                     /locus_tag="U4A12_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1774456.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4230..5171)
                     /locus_tag="U4A12_002110"
     CDS             complement(4230..5171)
                     /locus_tag="U4A12_002110"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN1774457.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5348..5935)
                     /locus_tag="U4A12_002111"
     CDS             complement(5348..5935)
                     /locus_tag="U4A12_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN1774458.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6156..7463)
                     /gene="obgE"
                     /locus_tag="U4A12_002112"
     CDS             complement(6156..7463)
                     /gene="obgE"
                     /locus_tag="U4A12_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN1774459.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7757..8767
                     /gene="gap"
                     /locus_tag="U4A12_002113"
     CDS             7757..8767
                     /gene="gap"
                     /locus_tag="U4A12_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1774460.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8852..9787)
                     /locus_tag="U4A12_002114"
     CDS             complement(8852..9787)
                     /locus_tag="U4A12_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1774461.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2717 a         2263 c         1706 g         3189 t
ORIGIN      
        1 taagaataac atcaagaata gcttcttttt tctgcttaat attccattaa tgtaatgatg
       61 tcatatcctt tgattttgtc gcgtccatgg agatccatca attcgatcaa aaatgcgcag
      121 cctacgacta caccgcctaa ttgttcgatc agttcgatcg tcgctttgat tgttcctcca
      181 gtagctagca aatcgtcaca aatcagcaca cgttgacctg gtttaatggc atccttatgc
      241 aatgtcaatg tatctgttcc atattctaat ccataagtca cttcgatggt ttctcgaggt
      301 aacttccctt ttttgcggac tggggcaaaa ccgactccta actcataagc aacaggacat
      361 cctacgatga atccacgggc ttctggccca actaccatat caattcgttt ttcttttgca
      421 taatttacga tttgtttagt agcttcccga taagcgtcac catctgccat cagtggggaa
      481 atatctcgaa acacaattcc ttttgacgga tagtcgggaa tactggcaat ataatctttt
      541 aaatccactc tttgtttcct cctaggttgt taaccatttc tttatggtag gaatatcgct
      601 caaaagcaaa aattcctcaa ctttgatttt attgagccgt cgctggtata atctgctctc
      661 agtcaaagga tgactttctg ggctagcgac tttttgtaaa acaccgttct ctattgtaac
      721 aaatttcaat tcaaaaaaca cctgtatcat aaaaattaat aatttttcag ggatacgcaa
      781 atattgagct attgctttta ttttatgacg tatatcaatt cgttcttgag aatggatcaa
      841 tttaaacaat tttgcgtact gttctcttgt gcctacccca ttcaaatagg cctcatcagg
      901 tgaaatcccc atcagataaa cacgattgaa tgaataagac cgaaaaactt ctttcagatc
      961 cgctaaatca tctggacaat ccataacaac tagagagttt ggttttcttt tttcaattat
     1021 tgcctttaca gaatctagtc cttcgaaaac aatcgcagct tcctggataa tctcaggcac
     1081 gaatttcaac gtcgaagaat caaatgctaa ataaaggcta ccttccgacg ttcggctctg
     1141 ttcacggaaa cgttttgccc gccaatcaaa tacttgcaga ccacccacag aaaaatcaga
     1201 taccatcagc tgcggtttct tacgcccgtt ccattcattg atcgaaagct ttcctaccac
     1261 atctacctga ttattcagca gttcttcttc ttgagtacca aaaccaaaag cgaccgcatc
     1321 aagctgactc gatgcatcgg aaaggacaaa tttcagatgc tgttggtttg ctccgattct
     1381 tttgatattt tctgcttgga cttgtcgaaa taaaaactga ggcaaaggat tatccgtacc
     1441 aaatggcgcc aatagtttca gctcatcaat tcgctctact gttacttcat ttggcagcaa
     1501 tacttcatca attctcaact caggccctct catcaagtca atttgatgat cgactatata
     1561 ttggttcatc ttctcctgta gtatagtgac attttcactt ggcatcgtta aaccgactgc
     1621 tgcatgatgt cctccgaagt aagtaaataa atctcgcatc tggtcaagca tttcaaaaag
     1681 attcaaggct tcaatactac gtccagatcc tttagctgat ccatcttctt ttaaagtaag
     1741 gacaagcgtt ggtttacccg tttcattcat tattttccca gcaacaatgc ccaaaacgcc
     1801 ttcatgccat cctcggttta ccaatagatg gatatgattc tccttattga ccatcgctaa
     1861 tgcttcttgt gttatctgct ctacgattgc ttttctttct tcattgatct cattcaattt
     1921 ttttgcttga acggatgctt cctcttcatc aaacgtcgca agtaacgaga cggcaggacg
     1981 tggatcgccc atccgaccga ttgcatttag tctgggagca atcgaaaaac cgatcgttgt
     2041 ttcgtctgcg tccctcattt ttacgccgct ttcttcaaat agcgcctgaa gtcctatccg
     2101 ttctgaatga tgaatggcat cgattcctaa tgaaacaagg attcgattct catcagtcaa
     2161 tgaaactaaa tcggcgatcg ttcctatcgc tactaaatcc aaaaattctg caggcggttc
     2221 ttccaataat gcagaagcta ctttgaatgc tactccgact cctgccaaat ctggaaatgg
     2281 atagtgcccc gctggatgac gagggtgaac aatcgcataa gcgtctggta aattctcagg
     2341 tagttcgtga tgatctgtaa tgatcacgtc tacaccagct tgctgagcgt atgctaccgc
     2401 atcatttcct gctaccccgt tatcaacagt gatgatcaac tggattcctt cttcgatctt
     2461 ttcttggtat accgattggt ttggtccata cccatgctca aatcgatttg gcaggaatac
     2521 ttctacttct gcaccaagta attccagtgt ttctttcatt accgttgcac tggtgatccc
     2581 atctgcgtca taatcaccgt aaacgaggat tttttctcca ctgattacag cttcttggat
     2641 tcggctgatt gctttatcca tatcgtgtat cagatacgga tcatgtaatt cttgttcctt
     2701 tggatgtaaa aatcggttga tatccttttc ctcatggtaa cctctttgcc acagtaactt
     2761 accgacaaat ggagataatt tcagttcacg gatcatttgg agaaaggatt cagaaggctc
     2821 tgtctcgctt aacttccaat caaaatttgc ttgtctcact ttctcactcc taacttagtt
     2881 attatagcaa aggaaatcag caaaaaaaag cagaaagaaa gaataaaaac agatcagatc
     2941 cgctagacag atgttcagca ggacctgatc ttttgtacga ccatttatga aatatcccta
     3001 ttagtcaaaa taattgaagc tttgtttggg cgattgagtt ttttgttctt ccggcgcttt
     3061 caattgactg atttgtgcgg ttttcgcttg taactccgcc tgataagcag cttccaactc
     3121 cgcacgttcg tttctaaact cgcgttgctg ttcttctttt gcttcttcaa ttttcttttg
     3181 aagttctgtt tgatattctg acatttcctg agtcagctgt ttgatttgct ttttttgctg
     3241 ccatagtgtt gccgaagcag tcaaaaatac aatcagtgca ccaatcagcg cagaaccgat
     3301 gatcactaag atcaatggcg cagaaaagga agtgaagcca aaacttacag gaacattttt
     3361 attgttcaat acagcaaaaa gaacaataat caaaactaaa acaaatccaa gaatcacatt
     3421 gctttgcttt ttcaattcct tcacctactt tttgttaaaa attccgctgg ctaaataatc
     3481 accaatcgtt ggaaacagcg tataaaattt tgatgcaact tctaaaataa acggttgatt
     3541 gatttcccgt tttggatgtc tcatattgcg gacgatcatt tttgccaact tggcaggttc
     3601 taaaacgatt tgtccgactt tttccaaata gcttccgctt ggatcagcct tatcgaagaa
     3661 atttgtctcg attggacctg gattcactgt cgtaacagct acgccaagtg gtttcaattc
     3721 taatcgcaaa gcgttggaga agccaaggac tgcaaatttc gtggcagaat aaaccgttga
     3781 ttttgcagtt gccatttttc cagccataga ggcgatatta atgatatgac cttgtcttct
     3841 ttctgccata tcgatcgcaa atttttgtgt caaaaccatc attcctaaga tgttgacttc
     3901 aaacatatcg tatgcttttc caaggtcgaa tgtaaggaag tcttcgaaaa tcccaaaacc
     3961 cgcattgttt accagaatat cgacgcgccc gacttcttcc atcacctttt catataattc
     4021 gtccacgctt tcaggattgc tgacgtctac cttgaaagca taagcctctt tgccgctcaa
     4081 gcgtctgcac tcgtctctga caccttctac aaatgccatt cttcgtgcgc aacttataac
     4141 gattgctcct tgctttgctg cttcataaca gatttgtgcg cccagaccgc ctgaactacc
     4201 tgttactaaa acaactttat cttctaaatt catgcctctt cctcctcgcg aaatggaatc
     4261 tcaatgctat caaaatcttt cataatcgtc gtgttttcaa acacttcttg tgcttctttt
     4321 tcaagctcta aagctgcttt tcctaaatat ctcgcactga tatgagtaag tagcagccgt
     4381 tttgcctgcg cttcttttgc aacttcagca gcttgatgag tcgtggaatg gaaatagttg
     4441 tgcgccattc ttgcttcatc tttattaaat gtgctttcat gtaccagaat atcgctattt
     4501 tccgcaagca caacggaatt atgtgttttt ctcgtatcgc ctaaaatagt cacgattcgt
     4561 ccttttttat ctggacctac gaaatctttt ccattgatcg tccgcccatc ttctagttgg
     4621 atcgtttcac cttgcttcag tttgccgtac agtggtccag ctggaatacc taattctttt
     4681 agacgatcca cctgcaattc tcctttatga tcatgttcaa ccacacgata accaaaactg
     4741 tcgatcccat gatttagttt ttttgtataa acagaaaatt gctgatcagt aaaaatcggg
     4801 gctgtttcat ttagctcaat gaatttcagc ggataactta aacgcgtttg cgaaatacct
     4861 aaagagactt ttatgtattc ctctatccct tttggcccgt aaatctctaa tggcgtatcc
     4921 ccaccttgaa atgaccggct gctgatgagc ccgggaagtc caaaaatatg atcgccgtgt
     4981 aaatgtgtaa tgaaaatttt accgattttt ctcggacgaa tagtcgttcg caaaatctgc
     5041 atttgcgtgc cttcgccaca gtcgaacaac caaacttcat tgcgctcgtc taaaagtttc
     5101 aatgctatac ttgtcacatt tctatgtttt gcaggaacac ctgcacctgt tcctaaaaat
     5161 tgtaattcca taatctacct gactttcttt aagttcctct ttaattttag ggtaaaaaga
     5221 agggataatc aaccgtaagt cgagaatggg ttcaaattaa aaataacgtt ctaaagcgat
     5281 agcgttttct ttgaaaatta ctataaaaac agcagcgaac ccgtttgaga tcctctgctg
     5341 ttttttatta atttgaagat ccagaacttt cattgtcgtt ggaagtttgc ggatgtttgt
     5401 ccttccaagt cttttgtgca gcttctttcg catgattttc agcttctttc gcatcatgca
     5461 catctttttc gacatcttca taatccaagc gagtaatttc tccgcctaat tccatcatta
     5521 ccaattgatt cagtgggcga ttgtcctctt catcagcaat caagatcagt cctgtagctc
     5581 cttcaccgat tttatttcct acaaagtcga acacggtctg ggcatctttt atttctttgg
     5641 catcttgtgt cgcaccgatc attcctccgg caaaccatcc taataaaatc cctagcggac
     5701 cacctaatac accaaccaac attccgatta atccacccgt agaagatttg ttattaccgg
     5761 taaagtcgat aaaatcttct attttgaatt gatgtgtccc attaggatca tggctgacaa
     5821 ctgccatctg ttctccttta atcgaacgaa cagcatgtaa ctttttgatt tctgaaaatg
     5881 cctgatatgc ctggctttct acttgaaaat ttaaaatgat tactcgtttg ctcattaaaa
     5941 acgccccttt cataccttct tgttatttat tttaccttta attgaaaaat aagaaaagaa
     6001 aagcactctt tttttccaat aaaatagggc tgtgacaaaa gtcgtatcac agctcctttt
     6061 tccgaataaa cggcgttctt gaagctgatc acttctgtca caacctccct taaatctgaa
     6121 tctatatttt ttggtatttg tttttatttg cttgcctatt cgacaaattc aaattcaaac
     6181 tcaccgatac gtacaaggtc gccatctttc gcaccgcgtg cccgaagagc ttcatccaca
     6241 cccataccac gtaattgacg agcaaaacgc atcactgttt catcatgatc gaaattcgtc
     6301 atttggaata atttttcgat cttctcacca gataagatcc acgttgcatc tgaatcacga
     6361 tcgatttgga actcaggacc ttctggattg aatccatagt gtacagtttc ttcttccagt
     6421 tcttcttcgt acaatgggaa ttctggcgtt acttctaaca aatctgctgt agcattcaat
     6481 aacgcgtcca atccttgtcg agtcactcca gaaattggaa atactgggat gtcatcagca
     6541 aattcatctt ctttttcctt gttcagctgt tctttgaatt tcactagatt ttccggtgca
     6601 tccggcatat ccattttgtt tgccacgatg atttgcggac gctctagcaa gcgtaagtta
     6661 taagtagata attctttgtt gatggcaaga tagtcttcat aaggatctcg tccttccatc
     6721 ccactcatat cgatcacatg taaaattact cgtgttcttt cgatatgacg aaggaattgt
     6781 gtacccagcc caacaccttg cgaagctcct tcaatcaagc ctggcaagtc tgctgcggca
     6841 aaacttctgc catcacttgt agtaaccatt cctagattag gtacgagcgt cgtgaaatga
     6901 taagcgccga tttttggacg agcagatgaa ataacggata gtaaagtcga tttcccgaca
     6961 gatggaaaac ctacaagtcc aacgtctgcc aatactttca attctagttc gatttttcgc
     7021 tcttgtcctg gttcaccgtt ttcagcaatt tccggtgcag gattccgagg agaggcaaaa
     7081 cggatattcc ctcgtccacc gcgtccacct tttgccacaa ctagtgtttg accattttca
     7141 attaaatctc ctaaaagagc accagtctcc gcatctcgga cagtggttcc ttgtggtact
     7201 ttaacatagg tatcttctga accacgaccg tgcatccctt tactcatccc attttcacct
     7261 ggctgcgctt tgaaatggcg attgaaccga aaatccatca atgtacgtaa cccttcatcg
     7321 acgatcaaga tgacatcgcc gccacgacca ccgtctccgc cagccggtcc gccgtcagga
     7381 acgtattttt cgcgacgaaa tgcaaccatt ccgtctccgc ccttgccagc tttcacatcg
     7441 atcgttactt gatctaaaaa catggacata attaagtcct cctattattc atttatttat
     7501 ttgactgcct gaatttccac aataaacaaa agtcttactt attttatcgg tgaaatctga
     7561 ttttgtctat catttcagca aaaagattgt ttaccttctt ctttttttat catttggtaa
     7621 ggtttccaac cttttttctg ctaccaagta taattttttg acaaaatatt ctttgcgtag
     7681 cagaataaca gtgtattaat tgtattaatt gaggatgccg tttcacagca cccctaataa
     7741 aggaggaact tttgatatga cagtaaaagt aggtattaac ggatttgggc gtattggccg
     7801 tctggctttc cgccgcatca aagaagtctc agatgatatt gaagtagtag caatcaacga
     7861 cttaacaagt ccaactatgt tagcacaatt attgcaattt gactctactc atggtactta
     7921 tcctggcaca gttactgcaa cagaagactc aatcgtagtg gatggcgaag caactcgtgt
     7981 ttatgcagaa cccgacgcaa gcaaaatccc ttgggcaaaa gaaaacggtg tagatatcgt
     8041 actagaatgt actggttttt atacatctga agaaaaagca aaagcgcatt tagatgctgg
     8101 tgtcaaacga gtagttattt ctgccccagc tggtgctatg aaaaccatcg tctataacgt
     8161 caatgatgat acattagatg aaaacgacaa aatcatctca gcaggttcat gtacaacaaa
     8221 ctgtttagca cctatggctt atttcttaaa taatgaattt gggatcgaag ttggtacaat
     8281 gacaacagtt catgcctaca cttccacaca aatgctctta gatggacctg taaaaggcgg
     8341 taatttgcgt gctgctcgtt cagctgctga caatactatc ccacattcta ctggtgctgc
     8401 gaaagcaatc ggcttagtta tccctgagct acaaggaaaa ttacaaggac acgcacaacg
     8461 tgttccagtc gttgatggtt cattaactga attggtttca gtattgaaaa caaaagtaac
     8521 tgctgatcaa gtaaacgaag caatcaaaaa acacacagtt gacaatcctt cattcgggta
     8581 tgatgaccgt caaatcgttt ctagcgatgt cattggaaca actcaaggtt cgatctttga
     8641 cccaacgcaa acagaagtaa caactgctgg tgatttccaa cttgttaaaa ctgttgcatg
     8701 gtatgacaat gagtatggat ttacttgcca aatgattcgc ttactagaaa aattcgctaa
     8761 cttgtaattt gatagatcaa tcgataaaag aagcctttga ctggatccag tcaaaggctt
     8821 ctttcttttc atcccctact tttaaactcg ttcaatgaac atattggctt aaaaattgtt
     8881 ggactacttg ctgatatttt tcaggatctg tcttgtatgc ttccgcatgc tcggctcctt
     8941 ttacaacata cttttctttt ggtcccttcg ttgcacgata gaccttatct aacatttcaa
     9001 atggtacaaa tgtatcttcg tctccatgta taaagagcat tgggcgctgg tttttcttca
     9061 attgttccac tgcatttgct tcaccgaaaa agtagccagc acggactttt gtcactagac
     9121 tcgttacttg cataagcgga aatgctggaa gactaaacat atcgtctagc tgataagcaa
     9181 gctcatcatg aacggaagaa taaccgcaat cttcaatgat tgctttcaca ttatctggta
     9241 gtttttctcc gctagtcatc ataactgttg ctgctcccat gctgacaccg tatagaacga
     9301 tttcttgcga ttttccattg ttttctagca ctttgtttat ccactgtaca tagtctttac
     9361 gctctggcca tccaaatcca atgtaatccc cttcgctttt tccatgtcct cttgcatcag
     9421 gaacaaggac attgtaaccc atatcatgaa acatctttgc gtaattggtc atcgtttcag
     9481 cgtttcccat atagccatga gccacaagag ctgttttttc acttttcgtt tcagcaggaa
     9541 gataaatcgc tgataatttc agaccgtctt ttgaagtgat ggtccaatcg cttcggtttt
     9601 cttttgaact aaaccattta tgtgcatccg tttctttgga tgtctgtgtt gtaccaggtg
     9661 tatcttcgtc taaaaagtct ttctcagaag gcacaacagc ataattataa aaataattcc
     9721 ctgcaaacac taacgcaaca atcagagcta aagcgacgcc taaaaggatt ttcatccaaa
     9781 gtttcataaa tcatccatct catttctctt ttttcatata accttgaatt agtataatca
     9841 aagaatctct actatgcaat gttcattttt ataac
//
LOCUS       DASJOX010000092         9835 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJOX010000092 DASJOX010000000
VERSION     DASJOX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.093"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..307)
                     /locus_tag="U4A12_002115"
     CDS             complement(<1..307)
                     /locus_tag="U4A12_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1774462.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     "
     gene            580..3051
                     /locus_tag="U4A12_002116"
     CDS             580..3051
                     /locus_tag="U4A12_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1774463.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3154..4890)
                     /locus_tag="U4A12_002117"
     CDS             complement(3154..4890)
                     /locus_tag="U4A12_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN1774464.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4887..5591)
                     /locus_tag="U4A12_002118"
     CDS             complement(4887..5591)
                     /locus_tag="U4A12_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1774465.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5722..6225
                     /locus_tag="U4A12_002119"
     CDS             5722..6225
                     /locus_tag="U4A12_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN1774466.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6185..6523
                     /locus_tag="U4A12_002120"
     CDS             6185..6523
                     /locus_tag="U4A12_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN1774467.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6544..7962
                     /gene="ffh"
                     /locus_tag="U4A12_002121"
     CDS             6544..7962
                     /gene="ffh"
                     /locus_tag="U4A12_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN1774468.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8074..8652
                     /locus_tag="U4A12_002122"
     CDS             8074..8652
                     /locus_tag="U4A12_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN1774469.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8656..9177
                     /locus_tag="U4A12_002123"
     CDS             8656..9177
                     /locus_tag="U4A12_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1774470.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9256..9810)
                     /locus_tag="U4A12_002124"
     CDS             complement(9256..9810)
                     /locus_tag="U4A12_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1774471.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3174 a         1818 c         2036 g         2807 t
ORIGIN      
        1 gttcatcaac taagtccgtt ttcgcagaaa aggtccttcc acaatcatgg cagagaaaac
       61 gggtacgttt taattctaaa atagttaact tttctttgac tttcaatatc tgagtgcgtg
      121 tcgtatagga accgttgcgg ataatacgaa ccgatcgaca gtgttcacaa taggaaggag
      181 agtcatccaa ttttccttta ataaactgcg cttgaacgcc tttatattct ttttcaatca
      241 gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt ctagtatgat
      301 tatccattga gagactcctt tggtaggatg acgttttggt cgactaaatt ttaccaaaag
      361 tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt
      421 ccaccaacac aaaatattat agaacccttt ttttttgcta tatgacagga atgaaaggtg
      481 tttgcaagct taatgtgagg aacactttcc atatctgttc tatactggat aggagaagtt
      541 tggaaatatc cagaaaacga aatcaatcga ggtagccaca tgaagaataa atggataaat
      601 cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata aagtcatccg tctttttcca
      661 tcgtataaag aaggattaac ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca
      721 aataattcag tcgatcccac attcaaaaca aatcagcaaa tcgtattgga aaacatattt
      781 acgtatttca atttgatctt tcttatctta gccattttgc tttgtttagt agaatcctat
      841 aagaacttga cttttttacc agttatcata gccaatacag gaatcgccat ttttcaggaa
      901 attcgatcca aaaagatctt ggacgaacta aatgttttaa atacagcaaa agtcaatgtc
      961 atacgagacg ggatagaaca aacaatagat atagaagacc ttgtaataga tgacattgtt
     1021 attttagaga ctggatatca aattccagca gatgcagagg tcgtggaagg gaaactgcaa
     1081 gttaatgagg ctttattgac aggcgaagca gatgaaataa ccaaagaaat aggtgatcca
     1141 ttaatgtccg gcagtttcat tgtttccgga aaagcttacg cccgattaga caaagttgga
     1201 gcagattcgt atatttccca gctgacagtc aaagccaaga caatgggaga aggagaacag
     1261 tcggagatga ttgcttctct taatcaattg atcaaatgga ttgggatcat cattattcct
     1321 atcggtatcc tgcttttttc tcaaagttac ttttttaatg gaaacacgat caaagaaagt
     1381 gtcgttgcaa tggaagcagc attgatcggt atgatacctg aaggactgta cttgctgacg
     1441 acgattgcat tagcattaag tgcgactaaa ctagcaaagc aaagagttct tttacacaat
     1501 atgaaaagca ttgagacatt ggcaagggta aatgtccttt gtgtagataa aacaggaacg
     1561 atcacagaaa ataaaatgtc tgttcaaaaa atgatcgtct ctaaaaaaca agaaataatc
     1621 agtcagtcag atcaattgga agcatgcatt gctgactatg caaaagcgat ggcaggagac
     1681 aatgcaacaa tggaagctgt taaagcatat tttacaaaaa ccactgatca atcctatcac
     1741 catgttattc ctttttcttc tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg
     1801 tatgttttag gagcgcccga aatggttttg cgcgatcaat tgccggaata tgcggaagag
     1861 tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct ttggtgtgta cgatgggatt
     1921 cttgaagaac cagtattgac agaagcagtc acaccattag gctatattct gattgctaat
     1981 cctatcagaa aagaagcaag agccacattt gattacttca aaaaacagca agtagccatc
     2041 aaagtgattt ctggagataa tccgctcacc gtatccaatg tagctgtcca agcagggatt
     2101 tcacatgctg atcagtacgt tgatgcatct aaattatcag aagaagaata tgaagcagct
     2161 gtcgaaaaat atacagtttt tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa
     2221 ttgataaagc gtaacaatac agtagctatg acaggtgatg gcgtgaatga tattctagcc
     2281 atgaaagaag cagactgcag tatagcgatg gcttccggaa atgaagccgc catgcaggcc
     2341 tcacaagtgg tcttacttga ttctgacttt tcacgcatgc cagaagttgt tgcagaagga
     2401 cggcgcgttg tgaacaatat tgagcgatca gccagcttgt ttttagtgaa gaatattttc
     2461 tcctttttgc tctcactgtt ttcagtgatc tttgctctga cctatccttt agaaccatca
     2521 caaatcacat tgattagttt gtttactatc ggacttccat ctttcttgct tgctctagaa
     2581 gaaaacaaaa agcgaatcag agggaaattc atcatgaatg tgatggaaaa agctgtgcca
     2641 ggtggactaa cagatatgat cgtagtcggt gcgttggtga tctgcggggt gactttgaat
     2701 ctaaataaaa cagatgtttc tacagcatct accatgcttt tgattgcagt agggttcctc
     2761 gttctctata aaatctgttc accgctgaat aaattcagaa gtcagatcat tcttttctgt
     2821 gctagcggta actttttttc ggtcatcttt ttgcataagc tgttttcaat caccggtatc
     2881 tcagctgtat cacttttgct gttgagcatt ctatttttct cggcagattc gatttttagg
     2941 catttgacca cattggtaga atatttgttt gaacgtaaaa atgaaaaaga gcaaaagccg
     3001 tttagtcgcg tagctaaaaa atttctatcg atatttaaac gagaaaattg acgaaaagta
     3061 tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct ttcgaagcag gatgagtcat
     3121 ccaactttaa aacgcacgag cagttgctct tttttacatt ttcggaatgg taacggtgaa
     3181 agtcgaacct gttccaagat gactgtctac agtaatcgta ccattaagga gttgcacgta
     3241 gtcttttaca atcgctaacc ctaatccggt cccgcctgaa tgtcggcttc gcgccttgtc
     3301 tacgcggtaa aaacgctcaa agatccgttc ctgatcctca atgtcgatac ctataccaaa
     3361 gtcttggaca gaaaacgata gatcatctgt tgcctgatag cggataataa tctcgctgtc
     3421 gctttttgaa tattggacag cattttcaat tagatttttg ataattggat aaaggatatc
     3481 tgttttagta gtaaatggtt cgttctttcc aatcaatcga atcgtcaacc gtttcttttc
     3541 gatgattggc tgataatttt gaacgatttg ctggaaataa ggttctgtat aaattgtctg
     3601 gatctcgtaa gaattctcgc catcttttga cagctggatg atttcccgaa tcagtttatc
     3661 taaccggatc gcatcttttt gcatgatctc aagaaaagaa gtcagagttt gcgggtcatc
     3721 ttttgcacca tccagtaaag tctcggtaaa accaatcagt gaagtcacag gagtttttaa
     3781 ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt tctaaacgtc gcactttcgt
     3841 taagtcatac gccaccccta gaatctgtcc ggtaccttct gaattagtaa agtagcgcaa
     3901 actcaagtct aatgtctgcc gaccttctgt aagggtgatc tcctgatgga tcagcggatg
     3961 ctcaggagtc acttgatgaa tcagctgaat gagttttggc tcttgaatga cttctgtgta
     4021 ccgctgttca ggttgatagc ttaccacgtt taaatgtagc tgcatctttg gattaagcag
     4081 tagtaaacga gaatcagcat catcaatcag gaatacgcca atcatcagtt cgttcagtaa
     4141 cgtatataat tgctcttctg tggtagtaca cgcccgatac atcttactca tttgttcgct
     4201 caacgcatta atcgtctgat acaattcttc ccattgatcg ggtgtttgca tgattacttc
     4261 cgcctgatta ggattttgca ccattttttt tagtacgggc agcacggttt tcaatggctg
     4321 gtttttttga tagatcagat aatagatcat tcctgcaaga acaaggaaga aagacagaaa
     4381 gaagaaaaag acccaacggc gaaaactctc agttctcggt aaaaaaccgc ttgtcggttc
     4441 tgcgatacgt aaaatggctt ctaactggcc actttttttg actggtaatg cgacatataa
     4501 aagatcctca ttcaaagtag tacttttgcg caaagcgcta cctaggttcc caccattcaa
     4561 tacggctttg atttcgggac gggagtttcg ctgttcattc aatgtttcat tgtttgtgtc
     4621 aaataagatc tttccagtag catccattaa agtaatccgt tcgttcgact gatggacgaa
     4681 ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt tcgacatcta attgccggat
     4741 cagcagttgt gctttttttg tcaggtagct ttcttgctgt tccacgatct gctgttgaaa
     4801 ataatgggaa atcaattgcc agccaccaaa aaacaaacta actaaaagaa taagaaagaa
     4861 gagacgttca taaatttttt tccatttcat cgtttgggtt cctgaaactt atagccaaat
     4921 cctctaatag tcaccaagta ctttgggtgt tttggatctc gctcgatttt ttctcgcaaa
     4981 tgactaacgt gtacatctac gattcggctt tgtcctgcga agtcaaaatt ccaaatgcgg
     5041 tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt tcatgaaata aacaagtaat
     5101 tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta cggtcacttg ataattggtc
     5161 aaatcagcag tgatttgtcc aatcgataga tattcaggct cagcttcatc ttttgattgc
     5221 ttacgaggtt caattcgtct gaatattgct ttcatacggg ctaatacttc gcgaggacta
     5281 aatggtttgg tcaaatagtc gtcagcacct atttctaggc caatgatccg atccacttgg
     5341 tcatctttag ccgtcagcat caaaatcggt gtttcatttt tttcttgacg cagccgctga
     5401 gtgatctcca tgccatccat cgttggaagc atcacatcta aaatgatgaa atcaaatgat
     5461 tgttctaatg ctaattctaa tccttcgcct ccatctgtgg cactggttac cttgtagcct
     5521 tctttttcca aattgaacgt caagagcgtt acaatagaag gttcatcatc aacaactagt
     5581 atttttttca ttcaaatctc ctttaaatag gtttcattct gtttatattt tagcatagaa
     5641 tcagtacaaa gggctatttt acaaaagaaa taaatggtat aataggaagg cactttgctg
     5701 aatagaaagg aagagcagtc aatgattgga atcagtgcat gtttaggcgg tctggcctgt
     5761 cgctatgatg gacagaaaaa agaagtacca gaattgatgg aactagttga aactggagaa
     5821 gctgtcatgg tctgtccaga agtgatcggc ggattaccaa ttcctagaga tcccgctgaa
     5881 atcattggtg gaaatggatt cgatgtgtgg aaagatcaag caaaagtatg gacgacaagc
     5941 ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca aagcctatca aaaattgcag
     6001 gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg aacccattcg
     6061 atttatgacg gaagtttttc cggaataaaa aaaaatggtg ccggagtggc tactgcttat
     6121 tttattcagc aaaaaatgac tgtgctctct gatgaagaat ggctgaaaac tagaggtgag
     6181 cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc tttgaatttt attcgacctt
     6241 attgacagaa aaacaaatga actacatgga attatattat gcagatgatt tttcattagg
     6301 tgagatcgcg gaagaatacg aagtaagccg acaagcagta tatgacaata tcaagcggac
     6361 aagcaagatc ttggaggatt atgaaaaaaa actccatctt ttttctaatt atattgttcg
     6421 agaacaggta ttggaaaata tgactgcata tattacagat aagtacccgg aagataaaaa
     6481 actacttgaa tatgtacagc aaattcaagc aatcgaagaa taaataaaag gaatgaagaa
     6541 aatatggcat ttgaaagctt aactgaacgt ctccagcagg cgatgggaaa aataagaaaa
     6601 aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc gggaaatccg ccttgcctta
     6661 ctagaagccg atgttaactt gcaagtagtc aaagatttta ctaagcgcgt acgcgaacgt
     6721 gcggtagggg cagaagtgct ggaaagttta tcacctgcac aacaaatcgt aaaaatcgtt
     6781 gatgaagaat tgacgatcac acttggttct gaaacagcag agttaaataa atcaccaaaa
     6841 ataccaactg ttatcatgat ggctggtctt caaggggcag gtaaaacgac ctttactggt
     6901 aaattagcca attatttaaa gaaaaatgaa aatgctcgtc cgctcctaat cgcaggggac
     6961 gtatatcgac cagctgcgat cgaccaattg aaagtattag gtcaacagct agacgttcct
     7021 gtatttgata tgggaactga agtcagcccg gtagagatcg tccgtcaagg gatggagtta
     7081 gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg caggacgtct gcatatcgat
     7141 gaaacattga tggatgagtt gaaacaaatc aaagaattga cgcaaccaaa tgagattttg
     7201 cttgttgtcg atgccatgac tggtcaagat gccgtcaacg tagctgatag cttcaaccag
     7261 caattaggta ttacaggggt cgtcatcact aaacttgatg gggatactcg aggcggggca
     7321 gcactatcga tccgctcagt gacaggcgca ccaatcaaat tcatcggttc tggtgaaaaa
     7381 ttgactgact tagaagtatt ccacccagat cgtatggcta gccgtatcct tggtatgggg
     7441 gatatgctga cattgattga aaaagcgcag caagactacg acgagaaaaa agcggaagaa
     7501 cttgcacaga aaatgcggga aaattcattc gatttcaatg actttattga acagcttgat
     7561 caagtgatgg gtatgggccc actggaagat ctaatcaaaa tgatcccagg aatgaatcaa
     7621 gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg ttgaacgcaa aaaagcgatg
     7681 gtttattcta tgacgccagc agaaagagca aatcctgatc tgttgaaccc aagccgtcgt
     7741 cgccgaattg ctgccggttc tggaaacagt gttgttgaag tgaatcgaat gatcaaacaa
     7801 ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag gggatatgaa catcccaggt
     7861 atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag gcaaaatggc gatgaatcga
     7921 atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat aagatgaaaa aaacagcttc
     7981 ggtacagact gaaaatgtct actgccggag cttttctatc agcaattttt attctttact
     8041 aatacaatta ctctaaacga agaggtggca cagatgagat gtccgtggag tgaacaaaca
     8101 gaaagtatgc ggcaatatca tgatactgtc tggggtgttc ctgaatatga tgaccaaaag
     8161 ctgttccgaa aattgatgct tgatattaat caagccggat tgagctggca gacgatttta
     8221 aataaaagtg aagcattcga tgaagcatat gatcgattcg atatcaaaaa agtcgcaaat
     8281 tatggagaaa caaaaatcag tgaactaatg gaaaacaaag gaattattcg taaccgaaga
     8341 aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga aaattcaaga agaaatcggc
     8401 agtttttcag agtatctttg gtcgttttct aatggaaaaa tcgttgattc ttcttatcat
     8461 tcacaagaag aagtcccaac aacaagtcct ctttcagatg aattagcaaa agatctaaag
     8521 aaaagaggat ttaaatttat cggaagcaca acaatctatg cttttttaga agctgtcggg
     8581 atcatcaatg atcaccttga cagttgtttc cgcaaagaag aggtcgtacg tatcggcaga
     8641 aagggcgaat aaagcatgcg aaaaatcata ttttatggtg ctataagttt agatggctat
     8701 ttggcaacga aaacggatga tcttcaatgg ctttttgaca ctccaacagg taagcaaact
     8761 acttatgaag ctttttatga aacaatcgat acgaccatca tgggcagaaa aacttatcag
     8821 gaagcaaaaa aatatttgga gaccgaagca atttatccag agaaaaagaa tttcgttttt
     8881 tccacccacg cacaattaca attgccagat gctgaggtcg taaacgaaga tcctgttgct
     8941 tttctaaaaa atctacaaat gacagaagga aaagatatct gggttgtggg tggcggtcga
     9001 ttgatcaaac cgctattgga acatcaaatg attgatgaat ggtatatcca gattacccct
     9061 gtattattag gggacgggat tcctttattc caaaaaggaa catatgaagc gcgttttgaa
     9121 atgctagaca cgacacgatt cggtgaattt atcgaattgc attatgtacg aaagtagaag
     9181 taaataaaat aaaaaaggca actatatgca gaaatgtcct gtacatagtt gcctttttta
     9241 ttaccgttat gctttttact ctgttaattt gttagaaaaa tctagatacg catctaaaat
     9301 cgtttggaag aaacttaatg ttccttcaac gatattacca ttttcatcaa ttagattcgc
     9361 aacgtttccg atatatgctt ccggttgctg caaggttggc acattcaaaa agacaagtga
     9421 ttggcgtaga tggtggtttg cgccaaagcc gccgatccca cctggtgatg cagtaacgac
     9481 taaacctggt ttatgatccc aaacactgtg tccataaggt cttgagccta catctaaagc
     9541 atttttcaat actgctggca cagaacggtt gtattcagga gtaacgaaaa cgacaccgtc
     9601 tagtcccctc acttcctcac gaaaacgagt ccattcagca ggagcctgtt ctggagtttc
     9661 gagatcctca ttgtaaaatg gcagatcatc gatttttaca aatacacttt cgtacccttc
     9721 aggaagcaat ttcgcaaaag cttctgctac ttttttgttg taagaatctt ttcttaaact
     9781 accaataaaa aatccaattt tttttgtcat acgatttttc ctccttcaaa ctatc
//
LOCUS       DASJOX010000093         9630 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJOX010000093 DASJOX010000000
VERSION     DASJOX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.094"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..676)
                     /locus_tag="U4A12_002125"
     CDS             complement(14..676)
                     /locus_tag="U4A12_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774472.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(689..2191)
                     /locus_tag="U4A12_002126"
     CDS             complement(689..2191)
                     /locus_tag="U4A12_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774473.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2739..4418
                     /locus_tag="U4A12_002127"
     CDS             2739..4418
                     /locus_tag="U4A12_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1774474.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4545..5024
                     /locus_tag="U4A12_002128"
     CDS             4545..5024
                     /locus_tag="U4A12_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN1774475.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5243..5923
                     /locus_tag="U4A12_002129"
     CDS             5243..5923
                     /locus_tag="U4A12_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN1774476.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5931..7262
                     /locus_tag="U4A12_002130"
     CDS             5931..7262
                     /locus_tag="U4A12_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1774477.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7277..8239
                     /locus_tag="U4A12_002131"
     CDS             7277..8239
                     /locus_tag="U4A12_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN1774478.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8350..8688
                     /locus_tag="U4A12_002132"
     CDS             8350..8688
                     /locus_tag="U4A12_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN1774479.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8767..9474
                     /locus_tag="U4A12_002133"
     CDS             8767..9474
                     /locus_tag="U4A12_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN1774480.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3058 a         1735 c         2071 g         2766 t
ORIGIN      
        1 attatcctgt tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt
       61 catcacagct ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt
      121 catgggcgat ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc
      181 cagtaggctc atccgctacg attagttgat gttcacagca gatagcacga acgatagcga
      241 ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa
      301 ttcctacacg agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag
      361 tgatttccat tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag
      421 cttggaaaat aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg
      481 aacgatcttg atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa
      541 gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga
      601 aattcagttc tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt
      661 gttctgtccg taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc
      721 ttaatatccc tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg
      781 ctaatagtcc caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa
      841 cagcaaatgg attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc
      901 tgttacctgg catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct
      961 gttggtcaag tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa
     1021 ctcctaatgc aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac
     1081 ttctggattc tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa
     1141 tcataatgat cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt
     1201 tagaaaaact tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg
     1261 attgtaagct aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca
     1321 ttttatctgg atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc
     1381 catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt
     1441 tgaatttcat acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca
     1501 tttcataagt ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca
     1561 ggtcatttgt ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt
     1621 catctattcc ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg
     1681 cagttgctga cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca
     1741 ttcctccagc aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag
     1801 aactagtgat ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat
     1861 aactctttac attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa
     1921 gagaaaaact gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt
     1981 cctgtttttt aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt
     2041 tttgtgcttg attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa
     2101 aaataagaat ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg
     2161 tacttttcca tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt
     2221 gataatagac aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac
     2281 atgaggttct gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa
     2341 aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag
     2401 atgctacaga gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt
     2461 agtttatttg ttgtataata atggggaaca gctgaatact tatataagta ttctggctat
     2521 agcgtttcgc aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca
     2581 actgtcagaa cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata
     2641 gtgattttgc atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac
     2701 atctttaaac ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc
     2761 cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata
     2821 tttttatttt agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg
     2881 taatccctga ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa
     2941 cacatggaca tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac
     3001 cagtattcgg gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag
     3061 cagccaaagg atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg
     3121 aaacagtagt gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc
     3181 tgaaaacaaa agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta
     3241 tcccaatgta tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag
     3301 ccttactaag tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa
     3361 tagcagatga agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg
     3421 tagcaagtaa tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa
     3481 aagttgtctt gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa
     3541 aacttcaatt accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc
     3601 cagaagaact gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa
     3661 aaatggcaaa cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca
     3721 ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc
     3781 gagctggtgg aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc
     3841 ctaatgactt gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag
     3901 gagagtatcg tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca
     3961 aaaatatcta tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg
     4021 ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg
     4081 gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg
     4141 tcactatcag tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat
     4201 tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa
     4261 tcgttgagaa acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc
     4321 gagatcaatt aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag
     4381 tcattatgga agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct
     4441 ctctatagag tgctcgtctt gctctttttt tgcgaaaaac aaataatttt tgaatacagc
     4501 atccctcgct ttataataaa cgatagaata aagggagtga atcaatgatg agtcaaacaa
     4561 aagtcgaacg ttatttagaa gaacaaaaga tcagctatca accacttgat tttagtcatg
     4621 tggtagaaga atctttcaca gaagaactac aaaaaagagg gattgatcca gctttgatat
     4681 gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg attcctttga
     4741 acgatcactt agattacaaa aaaacgagac aagtgacgaa agatcgtaaa gttggttttc
     4801 ctggaatgga ttttgtatta atgcatacag gatatcccca cggagcaaac acccctattg
     4861 gaataaaact cgcacatcct gattattgtt ttttagcaga cagttcttta aaagaaaaag
     4921 aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt
     4981 tagaaaagat cgtccatcct atttacgctg atttaattag ttaagggacg agaagagaag
     5041 ctaagcagag cagagacctc aaacttgaga ttatatctgc ttggtacttg gtgcatcctg
     5101 tattatctgt tacaattgct ttgtacatac tgtactatca atccaatgaa aatatattta
     5161 gcagctatat tttttcattg atgtgcaagt gatgaaatcg tttttaataa aaaagataca
     5221 gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat ggacattcct gtttaacaat
     5281 tgaaatggaa aatggccaac gcgtgatgat tgacccgttt attacaggaa atccattgac
     5341 agacttaact gcagaagaag taaagacaga ttggttgctg attactcatg gtcatagtga
     5401 ccacatagga gatatgattc ctattgcaaa agaaaatcaa gccacaatta tcagtattgt
     5461 tgagattgct gattatgcga acagcagagg agtagattca ttcggtatga atatcggtgg
     5521 aaaacatgcg tttccattcg gcgtggtaaa attcgtacat gcacagcata gttcaagcta
     5581 tgaagtagac ggtatcgttc agtatatggg cgagcctagc ggaattatta ttcaagcaga
     5641 aggaaaaacg atttatcatg caggggatac tgcttatttc agtgatctag gattgcttgc
     5701 agaagaattc gatattgatg tcgctttctt gccgattggc gataactata cgatggggcc
     5761 agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc caatccatta
     5821 caatactttc cctgtcattg aacaagatcc cgaatctttt gtagagttac tggaagatag
     5881 tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg taggcagaaa atggcgacaa
     5941 aacatgattt gattttagaa tatattgaaa gtttacctgt tggcaatcga atttctgtaa
     6001 gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg
     6061 cagaaaatgt cggactagtt tcaacaattc aacgagtagg gaccatccgt atcgaacgca
     6121 aattaaaaaa acatattgaa cgtttgacat ttggcgaggt cgtcaggatc attgaagggg
     6181 acgttttagg cggggcggca ggattagaca aggtcttgaa taaattcgtc atcggcgcga
     6241 tgactaaaca tgccatggaa cgctatatta cgccaggatc actaatgatc gtcggcaacc
     6301 gggcggaagt ccaaaaatta gcattggaag atggcgccgc agtattgatc acaggaggat
     6361 tcaatacgac tgctgagatt gcaaagctag ctgatgatct tgaaatgccg atcttacgca
     6421 cctcttatga tacatttaca gtcgcaacca tgatcaaccg agcgttaagc gaccaattga
     6481 tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc agctgaaaag actcattatt
     6541 tgcagcagac agatacgatt tctgattatc agcgtttgtc agaagaaacg cagcattcgc
     6601 gttttccagt cgtcaaccgc catcatcgtt tgatggggat tgttacggca aaagatgttt
     6661 tagggaaatc gccaaaccag atcatcgatc gggtgatgac gaaagaacca atcagtgtaa
     6721 agaaaacaat gagtattgcc tctgttagcc atcaaatgat ctgggatggg ctagaagtca
     6781 tgccggtcgt ttccgatgac ttgacattgg aaggcttgat tacccggcaa gatgtaatga
     6841 aagcgatgca gcttgtccag cgacaaccgc agattgcaga tacgatttcg gatcagatat
     6901 ctggaaatat ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt
     6961 tcaaattcgt agtagcacca caaatggtca atggcgttgg aaccatttct tttggcgtac
     7021 ttagcgaaat catttctgat gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata
     7081 tcttgattga acaagtgaac ctacattatt tgcggctgat ccaattggaa agcgaattag
     7141 acattcgtcc acgtgtattg gaaatcggca gacgttccgc taaactggat atcgaagtgt
     7201 ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt
     7261 agaaagcagg agtgaaatga cagtacagca agaaatagta gatacaataa aaaaatacga
     7321 taaaatcatt atccacaggc atttgcgccc tgatcctgat gcattaggat cacaagtagg
     7381 attagccgaa ctattacggg aaagtttccc agaaaaagaa atacgtcaag taggtgaaac
     7441 gattgaaggg cttcgctttt tagcagaaat gcaagaagtt gaagatgtct tctaccaagg
     7501 agcgctagtc atcgtgactg atacagctaa ttcacccaga atcagtgatc aaagatatca
     7561 gttggcagaa aaactgatca aaatcgacca tcatcccaat gatgaccctt atggagatct
     7621 tgtgtgggta aatacaaaag caagtagctg tagtgagatg attgcagaat ttgctttgat
     7681 gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg ttactttatg caggaatcgt
     7741 cggagataca gggcgctttt tgtatccctc cacgacgtcc cgtacattag agattgcttc
     7801 gatattgaga aattatccat ttgatgcaag tgctttgaac agagagattg aacaaatgcc
     7861 gatgaaagtc gctaaacttt ccggttatct ttatcaaaat atccaggtgg atgaaaatgg
     7921 tgcaggaaga ataatccttc cgcaagaaat tcttgataaa tatggaatcg atgattcaga
     7981 gacagcttcg atcgtttcac ttcctggagt gattgacgaa gtgctggctt gggggatttt
     8041 tgttcagcag ccttcagggt attaccgcgt acgtttacgt tctaaggggc cgatcatcaa
     8101 tgaattggct aaacgccatc atggaggagg ccatccatta gcaagtggtg cgaatgcaaa
     8161 agatttagca gaagtagagg aaatctataa agagatccaa gatatatgcc aatcgtataa
     8221 gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa
     8281 atgtatcttt tcaagatctt ctttttccgt tacaatagtg ctcactaaag attttaaagg
     8341 agaaagaaga tgacattacc aaattgccca gaatgtggct cagagtacac atatgaagat
     8401 cgaggcttgt tgatttgccc agaatgcggg aacgaatgga acaaagaaga acaagaaact
     8461 gtcatagaag acggattagt ggtattagat gcaaatggaa accgtctgtt agagggcgat
     8521 agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt caggtgccat taaacaagga
     8581 acaaaagtca agaacatccg tttagtagaa ggcgatcata atatcgattg caaggtagaa
     8641 ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag aatattcaag
     8701 gtcttttcac attcttttcc tactactaat gataaaataa agtaaacgaa aaggagtgaa
     8761 acgaaaatgt atggttatgg aatgtatggt tttttagatc cgacatttct tttggtcatt
     8821 cttggattag ttatttccgg aattgcgtca gcgtacgtca atagtacttt ccgaaaatac
     8881 gatcaagtga gaagcaaaaa caatgtgact ggtacacaag cagcacaata tatattacaa
     8941 agtcaacaga taaataatgt cggtgttcaa caaattgccg gagatctcac agataactac
     9001 aattcaggta ataagatgtt gagtttgtct caagcaactg cacagtctac ttcagttgct
     9061 gcgataggtg tagcagctca tgaatgcgga catgcggtac aagatgcttc gggttatgtc
     9121 ccattgaaga ttcgtgcagc acttgttcca gcagctaact ttggttcgat gatttcgttc
     9181 cccttgatta tggtaggtgt attgcttagc tggaactcta cgttaatcaa tatcgggatt
     9241 ttcgcttttt ctttagcatt acttttccaa ttggtgacat taccagtcga atttaacgcg
     9301 tctcggcgag caatccaaat tttgagtgaa ggcggactac tgacggaaga agaagtaccg
     9361 atggcaagac acgtattatt tgcagcagca ctgacttatg ttgcagcagc gctttcgaca
     9421 ttcctgcagc tgttgcgctt gatcttattg ttcggcggca accgaagaga ttaaaataaa
     9481 gaagttgtga aatcagcgct ttgatctgag ctaataccga gagttagctg attgaatacc
     9541 atttatttgg aataagggct ctttgtcaat aaggactgat gggttgtaca aaatcaaatc
     9601 tgggtcagaa tgaacctcat tctggctcag
//
LOCUS       DASJOX010000094         9600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJOX010000094 DASJOX010000000
VERSION     DASJOX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.095"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            170..2098
                     /locus_tag="U4A12_002134"
     CDS             170..2098
                     /locus_tag="U4A12_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1774481.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2236..2691
                     /locus_tag="U4A12_002135"
     CDS             2236..2691
                     /locus_tag="U4A12_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN1774482.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3161..3430
                     /locus_tag="U4A12_002136"
     CDS             3161..3430
                     /locus_tag="U4A12_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774483.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3830..4084
                     /locus_tag="U4A12_002137"
     CDS             3830..4084
                     /locus_tag="U4A12_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN1774484.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4173..5219)
                     /gene="holA"
                     /locus_tag="U4A12_002138"
     CDS             complement(4173..5219)
                     /gene="holA"
                     /locus_tag="U4A12_002138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1774485.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5257..7530)
                     /locus_tag="U4A12_002139"
     CDS             complement(5257..7530)
                     /locus_tag="U4A12_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN1774486.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7523..8062)
                     /locus_tag="U4A12_002140"
     CDS             complement(7523..8062)
                     /locus_tag="U4A12_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN1774487.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8198..8416)
                     /locus_tag="U4A12_002141"
     CDS             complement(8198..8416)
                     /locus_tag="U4A12_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN1774488.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8413..8658)
                     /locus_tag="U4A12_002142"
     CDS             complement(8413..8658)
                     /locus_tag="U4A12_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1774489.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8774..9502)
                     /locus_tag="U4A12_002143"
     CDS             complement(8774..9502)
                     /locus_tag="U4A12_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1774490.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2970 a         1863 c         1665 g         3102 t
ORIGIN      
        1 agaaatgttc tggtttttta ttttctttaa taataatcat tataaataat aaaaacaaca
       61 tcaagcttaa tcaccaaatg ataaccatta tcgttctcat ttagctgtta ttattatcaa
      121 ttacattttt attatgctat ccttgttttg acaaggaggt ctaataacga tgactaatca
      181 gaaaaaagct tttgcagcaa caatcggctg ccttttattt atgatcttcg gttttatctt
      241 cgaccgattc tctcttacca tcaccccact tttttatatc agtgccatta tcatcggtgg
      301 atataaacaa acatcggaag gcttgaaaga attgtggaat gatcggacac tgaatgtgga
      361 tttattgatg gctttagctg ctattggtgc ctgtttgatc ggtaattact ttgaaggagc
      421 aatgttgaca ttcatctttt gtttaagcgg tgcattagag gaatacacaa ctaataaaag
      481 tcaaaaagaa attactgctt tgatgaatct tcaaccacaa aaagcccaat tattaaaaga
      541 aaatggacaa atgcaagaag tagatgtttc tcaactaaag gtggataact tggtatttgt
      601 cgctaaagga gcagctgttc cgatagatgg ctttttagaa agcacacagg caacgatcga
      661 tgagtctgca ttaagcggtg aatctgtgcc tgtagaaaaa tatttaggcg atgagatgta
      721 tgcaggaacg ctgaatcaag gaaatcccat cacgattcga gtaacaaaaa cgagttccga
      781 aactgtcttt gctaagatta tccaattagt agaagaagcg cagaatacgc caactcaaac
      841 agcttcattt atcgaacgaa tcgaaaataa ttatgtaaaa ttgattttgc ttgctgttcc
      901 tctaatgatc cttctccccc actttttcct aggttggagt tgggatgaaa gcttttatcg
      961 aggaatggta ttattagttg tggcttctcc ttgtgcgctt gtcgcatctg ctacaccagc
     1021 ttctctagct gctctttcaa attctgcaaa aaatgggatt ttaatcaaag gcggtatcca
     1081 tttagaaaag ctttctgaat taaaagcgat tgcctttgat aaaacaggaa cgctcactaa
     1141 aggaaaacca gttgtaactg acagcttttt ctttgatgaa aaggatttgg cacaacaact
     1201 tcttgttgct atggaacaaa aaacgacgca tccacttgct catgcgattt tgcaatattt
     1261 cgattgcaca atacctgaag aaatccaata cttaccaata gaagaaatta ctggttttgg
     1321 attacaaact acctatttag gtgcgcaatg gaaagttggc aagcacgcat atgatcctga
     1381 gacgatgata atcagcaaag agattgcgga aatgatcgaa cgtttggaaa atcaaggcaa
     1441 aaccgtgatt tatctttcta aagatcagca gttaattgct gttttaggtc tgctagatat
     1501 tccaaaagca aatacccaac aagtaatttc ctactttaaa tcccaaaaca tccatacaag
     1561 tatgataacc ggcgaccatt ctggcacagc aaaggccatt gctgaacaag tagggattga
     1621 acattactat gcaagctgta ctccggaaga aaaaacacaa ctagtcaaaa aagagaatga
     1681 actttaccaa gtaaatgcga tggttggtga tggagtcaat gatgcgcctg ctttagcagc
     1741 cgcttcttta ggtgtcgcta tggggcaagg gacagatgcc gctatggata tcgcagacat
     1801 cgtcttaatg aaaagcgact taaataaatt tgtctacagt catcggctat ctttaaaaat
     1861 gcggcggatc atcaaacaaa atattatttt ttcgattctt gttattctct tgttgattgc
     1921 ttctaacttc ctgcaatttt taaatctacc tcttggagtt gtaggacacg aaggaagcac
     1981 aatattggtt attctgaatg gcttgcgttt actaagacct ttacctgatc tttctgattc
     2041 ttcaaaaaaa tgtaagtctt gtccactata caaagcgcgg ctgtcttctg tcaaataagc
     2101 aaagtgtttt tttatgattt tgtttatttt atgaaaaaaa cagcttatga ataggtcttg
     2161 aaggatgaat tcctactttt gtaactttct aaaaacatgt tatgattgtt tcgaagaaag
     2221 tgaggaatat atttcatgga aagttggctt tttttaggaa ttattttact tgtagcaatt
     2281 ttagctaaaa atcaaagtct gatgatagcg gcagcttttg ttttgattat gaagctcata
     2341 ccagcctccc gttccttttt cccttggctt caaggcaaag gaatcaatat tggggtgacg
     2401 atcatcacag cggctattct aatccctatc gcaacaggag aaatcggtct acgtgatcta
     2461 ttagaagcct ttaaatcccc tattggctgg gtagctgttt tttgcggtgg gctagtggct
     2521 gtcttatctt ccaagggtgt aggattgatt gcttctgatc cacaaatcac tgttgcactt
     2581 gttttcggca cgattttagg tgttgtcttc ttaagaggaa tcgccgccgg accggtcatt
     2641 gcttctggta tcacttactg tatcttacaa gtatttgctt taggaaaata gaacaaattt
     2701 tgtctatttt aggagattct taacaccccc aatttactga tccgtgatat actgacaacg
     2761 aggtgataca atgttcgaaa aattaaagta tttcatagga acaatcggct ggtttcttct
     2821 agcggtactt ttgattctgg gcttagctta tctattattt cctgatttga tacaaaatgt
     2881 gtttgatact gctttttgat tgacctatta aaacgatata ggggatgctc ctgcgatgca
     2941 ggggcttttt ttaatcaatg ttttgtaata atttaggccg tgccaatgag acttcctata
     3001 gaattcgatg ctcttttttc tttttggctc ttctgctctt ttttgttata ctagattaca
     3061 gtggacaaaa gaaagaatga tcgcatccac ttcaacgaat atcaaaatgg aagaattagt
     3121 gcctgttttc aggttacttt gaaaagaaag gaagacgctt gtggaaactt ttgaaggtct
     3181 tttaattgat acgtacgttt tacaagaaca gccaatgatg ctgactggga tcaacttgca
     3241 gcttgaggac cgtgtgatca atggcctgct taaagatcgc tatttggcaa aatatatctt
     3301 acgtttgccc ctaaataaat atgtgctgga agtaagtggc aaatggaacc gtcggaacca
     3361 gttggtcata aaaaaaatgt tgataaaaaa tatcgatgaa tacattaaaa taatcggtac
     3421 accagaatag cctttactga tcaaaggata ctggttataa aataactgca gtttcttaat
     3481 agaaaaacaa agaacaacag ggaatcgtat cccttttcaa aaaaaacttt gcaaacggtt
     3541 ctctttgtta tataataact tagtaccaaa cattcccatt caaactttgt ttaaatagat
     3601 aaataaaaat ctagtttggt ttgacgctat tgcctcccac aatagcgtct ttttgatttt
     3661 tttcagcttg tactatccgt ttttactgaa aaaaaatcct aataaaagac tgttaaggaa
     3721 aaaaacttgt catgttcata ttgcaaagca cgctgccttt aggtataata atgaatgttg
     3781 aattatgcgg taacacgcgt aagaacttaa gttatcggag gtgaaactaa tgccaaacat
     3841 tgaatctgca atcaaacgtg ttcgtacaag cgaaaacgct aatgtgaaaa attcatctca
     3901 aacaagcgct atgcgtactg caatcaaaaa atttgaagat gctgtagctt caggtgctga
     3961 caacgtagat gcgttatata aagaagctgt taaagctatt gatatggctg aatcaaaagg
     4021 tctaatccac aaaaacaaag ctaaccgcga caaatctcgt ttaagcaaaa aaatcgctaa
     4081 ataaaagaat cggcttcggc cgattttttt atttgtcaaa aaaaggacaa aaacaaacga
     4141 ccttcccgca tgctttgccc tcgtctttct tcttatcgat ttcttaacaa ctgccctgtg
     4201 cgcaaaacga atagttgaaa acttaattct ttttcgattt gtcccgtttt gatttgataa
     4261 tccatctcaa ctaattgatc aaacaactgt accagcaaat gttcatcgaa tttttttact
     4321 tcctgcatgg caagttttac acgatatgga tggattttca atgtttcagc aatatttgcc
     4381 tgctgataac caatctttac aagaaatttt gtctgtaaaa ataaacgtaa ttgagaaagc
     4441 aaaatcgcat taattttgat tgtttcttcc ccttgcataa ctaaatcttc gtataaacgc
     4501 aaagcttgtt ccgtcttccc ttttaaaata tattgggtca tgtcaaaaat attatgttct
     4561 aacgttttag gaactaattc ttctacttct cttaccgtga tttgctgttg attttgtcca
     4621 taaaggacca atttctgtaa ctctctcatc attttggata aatctaagtc ggtcaacctc
     4681 aagaataaat caattgcact tcgttccatt ttgattgagg aattctctaa ggtattcatc
     4741 agatattgac gtacttcttt ttcattcaac tgttggacat tgatgataat tgctgttttt
     4801 ttcaattgtt tcgttacttt tttccgttca tccaactttt catatggcgc aaagaatact
     4861 aatacagtcg attctaatgg ttctttcaaa taagccgtca ataaatcagt attttgttcg
     4921 attccattgt ttactttttc acctgttaaa aaataaggat tctctacgaa tactaaccgt
     4981 tgatctccga aaaacggcaa cgtctctgct tcagctacta cctcatctaa acttgcattt
     5041 tccatgtcaa aacttaaaaa atttaagtca tcttcgccct ctaattggat ttttttgaca
     5101 atttctgtcc gaacttgatc ttggagaaac ttttctgttc ctaaaatcaa gtaacaagat
     5161 gcaagttttt ctttacgtat tttttgtaac tctgcttgta tactcaaatt actcaccact
     5221 ttcaaaaatt atcctaccat gagcagatga gaattactag tctatcattg tttccactag
     5281 cccatgagga tgaaaaactg gatgccagcg ataggtgatc attccttgtt gatccgttcg
     5341 ccatgttttt atctgatttt cttctagtgt ctccaatact tcgctgtgcg ggtgaccata
     5401 acgattattt acgccgcagg atataatccc atgttttgct tcaatatggg aaagaaaatc
     5461 tgtgttactt gacgtacggc taccgtgatg acctaatttg actacatcgg ctttcagatt
     5521 aggataaatc aaaagcaatt gtttttccat tgcttgattc aaatctccta aaaataaaaa
     5581 gcgcctattg ttatattcca tccatagacc aatcgaatct tcatttcttc cctctccctt
     5641 ttcaaaagga gtcaatacat aaagatcata ataaccgctg atcctgtccc catgtttgat
     5701 ttcaatgatc tcagtaccat ttttttgagc ttcagataaa tgcctttgta atggctcatt
     5761 tcgtccagcg ccccaaccga tataaatttt tttgatagta aaatgctgag ataacgtggg
     5821 taattctccc acatgatccg tatcgtcgtg tgtcaagatg agtttatcga ttacacttat
     5881 cccctgacct tttagatatg gaacgatatt tctttcagca ggaggacgta tcataccttg
     5941 cgcccacttt tctctttcaa agtgtaactg acctccagta tcgatcaaaa tcgtttcttt
     6001 tcgaaaagga ctttgcaaaa agatactgtc tccttgaccg acatcgataa aagcgatcgt
     6061 tgaagaaaag cgtaaaaatg gcaaagtcaa aaaaataaaa gaaaaaagac caacataaaa
     6121 caagtgtttc acaggttgtt ctttcaacct atccatacaa atcagtaatc cgaaaatata
     6181 catcccagat agcacaaggg gcaaacgtcc tataatgaga gacgaccctg aagaaaaatc
     6241 gattattcgc tcaaataaga ttaatccctc ttctatcagc aaaagtaaag tttccggcag
     6301 ccagcgattg aagagtgtga gaaataacac accaggaaga aaaagaaact tgaataccgg
     6361 tgcaatcagt aatgtaaaaa gactacctaa aataggccac tcataaaaat gccatacaat
     6421 caacggcatc gtgatcatag taaacaggac gtgagaggcc gtttttttca accaatctcc
     6481 atcaaaagaa atcaggataa acaaaaaact catgaaaaaa ctaagctgtc cacccgcatg
     6541 aaaaagaaat cgaggatcaa atagtaaaag aagccaaaag acaactgcaa ataagtccat
     6601 ggtggaataa tgcttattca gcaatttgac tagcaatcgc aataaaaaca gcaaactagc
     6661 acgcaaaaca gaaatagaac ttcctgcaat agaaacataa cagatagtta gtagactcaa
     6721 cggaataata ctgacgtgta gtggccatcc gatacgtcga aataaataat acagccaccc
     6781 taaataaaat tggatgtgta gacccgatag actgaacaga tgcagcaaac cactctccct
     6841 aaaaatttct tctggttctc tgaatcctgc atctttatag ccccataata aagaattaat
     6901 atatacactt gttttttgcg gaaattcaga ttggacaaag gagataaaag atcctctttt
     6961 tctctgaagc cagtttcgcc agtgattttg catggtgatc tgttcaatct tttgaatttg
     7021 gaaccgtcca gaaattctat ggttcctgaa gtaagcttct tggtcaaaat tataaagatt
     7081 ccgctttttt gctggagaga tgaactcgcc ttcagcagtc aattctattc cctttcctcg
     7141 gaagttaagc catgttttca attctgcttc tgtgtttact tgataagaaa gctggagctt
     7201 ttgtttattc tctgataacc ttccttccat tgtcaaaaaa ttaccatttg cacgaatcgt
     7261 atctgtaaat acagtaattt gcgcagatgt tgttttttcc tctaaaaatg aatactcttg
     7321 tagcctataa gaaatggata ccgttatgct acacaaaata cacaagtacg cgataaaacg
     7381 tccttctttt agcgaaaacc ataaaaaaat tccggcaaga agcaaacgat aaccccaatt
     7441 gtcagtgtaa tacaacaaac agataatcat cgacgtaatt gccggaaaaa tcaaccgatt
     7501 tcgaacatgc ctttctaaaa cgctagacat gttttcctgt taattcattt tgaagttttg
     7561 aaaaatgctg tgagtctaaa gcaacttggt gtacagatac acctaattgt tcgatcaacg
     7621 cttgggcata aggatcattt cgatagtcat ggagataatt gatccctttc acaccagctt
     7681 gcaacaaagc ctttgtacag gccaaacatg gaaaatgtgt gacatagatt tctgaacctt
     7741 ccgtcgggat accaagtttt gcacactgta ggagtgcatt catctctgca tggatcgttc
     7801 gcaagcagtg tccatccacg acgtaacacc cttcatctat gcagtggaca tctccactga
     7861 ctgctccatt atagccgcca gcaatgatcc ttttttctcg aacgagtgtc gctcctactt
     7921 ctaaacgtgt acacgtacta cgtaaagata acagtactgc ctgagccata aagtactggt
     7981 cccaaggaat gcgttcatta tgtgacttat cagtaacttt ttctttattc gtatcgcatg
     8041 cttttgaaga tttttctaac atgttttata ttcctccgtt ttttcaagtt gattaaaaag
     8101 tactcattgc ttaaacaaaa tattctttaa atataacgaa tcatcctgtc tgacaaaaga
     8161 tcctaccctt cactgccgaa atgaagtccg tcatccatta gcccattata aaaattcttc
     8221 gaatccattt gttcaagaaa ttccgtataa aaatcgatac ttcacaatca tggcgttttg
     8281 ctacttctgt gagaacagag atatattctt tgatcaactt gttattccga tatttttgtt
     8341 ttgtctcatc gactgctggt ggcgtgataa agaaagcatg atcagaacta tagtcttttt
     8401 tcagctgaac tatcatccac tgcagatttt ttggtacgtt tctttagaaa gttgtttatg
     8461 ctctgctaga tcattcgtcc caatgagaat aaatacttta tctgctgtat agctaagtac
     8521 atctttggca aagcgttctc gtagagcttg actgttattg ccgctgacag ccgtattcat
     8581 aatatgaatc ccagatattt tattttggag atagacattc agcattggtt tttttaatgt
     8641 ttctttgcga acaatcaagc tatttcctat ccaaaaatac tacctcctcg tcctgccaag
     8701 ctcttagaat tggattattc ttagtatcca ttagcgtcca tttattgctc gtgccagtcg
     8761 attttgaatc attctattta attgtaacga agttttttag tttttcaact gtcttttcac
     8821 cgataccaga aatctcctgc aggtcttcca ctttctggaa ggatccgttt tcttctctaa
     8881 atcgaatgat ttcttgcgct ttgactgatc cgatcccagg aatctgctga agttctgatg
     8941 gagtggctgt attgatattt attttgggag gctcggaatc tgctgtaggc tgttttcctt
     9001 ccgaggatgc caactgatca agatgttctg gcagctcttc acctacagca gggatatata
     9061 ggacttgctg atcttctatc ttcgcagaaa agttgattcg atcgctatcc gcttcttcta
     9121 ataatccacc tgcacgcttt agtacatcat agacccgttc ttctgaagaa aaggaataga
     9181 taccaggctc cttcacactt cctttgatat cggtgtacat gacctttgga cgagcatcct
     9241 cttctacatc gtttccttca gctgatggat cttctccact ggaactaaac gaagaaagta
     9301 tttcttttgc agcagtctct tctgtttctc tataaaatag caaatttgat aataaaaaac
     9361 cacttgcgat acaaaagata agaactatca tagacatcca aatgatcaat ttgggtgaag
     9421 ctagtatttt cttgctgatt ttttctatca ttgttctttt ttcaaagaat gaccagtaaa
     9481 aatcattcat tactcctttc atttaaaatt gaagaaacag tgagctctgg caaaatcgtc
     9541 ccttcaacat ctaattacgt caattttttc ttttgttttt atttttatcg tgaagatttt
//
LOCUS       DASJOX010000095         9598 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJOX010000095 DASJOX010000000
VERSION     DASJOX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.096"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..646)
                     /locus_tag="U4A12_002144"
     CDS             complement(<1..646)
                     /locus_tag="U4A12_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN1774491.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKK"
     gene            complement(648..1412)
                     /locus_tag="U4A12_002145"
     CDS             complement(648..1412)
                     /locus_tag="U4A12_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN1774492.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1420..2097)
                     /locus_tag="U4A12_002146"
     CDS             complement(1420..2097)
                     /locus_tag="U4A12_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN1774493.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2134..3519)
                     /locus_tag="U4A12_002147"
     CDS             complement(2134..3519)
                     /locus_tag="U4A12_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1774494.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3488..3742)
                     /locus_tag="U4A12_002148"
     CDS             complement(3488..3742)
                     /locus_tag="U4A12_002148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774495.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(3729..3813)
                     /gene="ffs"
                     /locus_tag="U4A12_002149"
     ncRNA           complement(3729..3813)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4A12_002149"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3836..4351)
                     /gene="tadA"
                     /locus_tag="U4A12_002150"
     CDS             complement(3836..4351)
                     /gene="tadA"
                     /locus_tag="U4A12_002150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN1774496.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4458..5315
                     /locus_tag="U4A12_002151"
     CDS             4458..5315
                     /locus_tag="U4A12_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774497.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5396..5731)
                     /locus_tag="U4A12_002152"
     CDS             complement(5396..5731)
                     /locus_tag="U4A12_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774498.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5798..7168)
                     /locus_tag="U4A12_002153"
     CDS             complement(5798..7168)
                     /locus_tag="U4A12_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN1774499.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7246..7782)
                     /locus_tag="U4A12_002154"
     CDS             complement(7246..7782)
                     /locus_tag="U4A12_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1774500.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8091..9572
                     /locus_tag="U4A12_002155"
     CDS             8091..9572
                     /locus_tag="U4A12_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774501.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3218 t
ORIGIN      
        1 tcttttttgt ttgtttcaag ctattttttc ctaagaaaag aaaaacagca aaaaataatt
       61 ctaataatac aaaaaataag ataaagctat gcatgaaaaa ttcttctgga agtacattga
      121 tgattggatc agtaacggga tcgaataacc agtcatcgtt gttgaaaaac gtttcatgaa
      181 aaaggataaa aaagcgatca aatcctatca tcatgaaaaa accgaaaaca actggcagta
      241 acatgccgat ttgaaacgga cgaatcaacc gccaaagccg atcattcttt tgcaaatatt
      301 tgataaacaa aatactcggg atcaaagtga tgaaaaacac agcataatcc actaaaaaca
      361 aaattttcac ttcataaaag tgatgggctc cgctagcaga aactgggaaa tctggcaagc
      421 tgaggatggt tttgaaggga ttattgagat aattcattag gtgatcaaaa tttttcagta
      481 acgtctcttg atctaaagaa gtataatcca aaatttgcaa atgtccgata tcaaatacgt
      541 atagtggtcg aaagttgatc gtcacaccaa ctgccagact gatcagtgtc agaaataggc
      601 tgaacaatcc agcatattcc atccactgcc atctcgaatt tttcatatta gaagctccat
      661 tcatccagtg attgtttcac ataggtagct gggataggca acccaggtac atcttcttct
      721 tttgtaaaac ctgataaaac gagcaaagta tctatattat tgcgtatccc agcttggata
      781 tctgtttcat agttatctcc aaccatgatt acttcttctt tttgcagccc taatacttca
      841 acggctttat ccataatgat ggcttctggt tttccgatgt agatcggcgg cgtttgggtt
      901 gctgtctcta caaaagagat gactgaccct gctccaggaa gaaggccgcg ttcagtaggt
      961 atattcttat ctgggttcgt tccaatgaaa gtagcacctt tttgaatagc aagtgttgct
     1021 ttaactactt tttcataggt cagataatta tctaagccta ccactacata gtcaggagcc
     1081 tcttcttccc atgtaaatcc ggcagctaaa atcaagtcaa tcaatcctgc ttctccgata
     1141 acatagactt ttttcccttt tccatctgct ttcataaaat cgattgttgc caaagtagct
     1201 gtgtagactg tctctggagc tacatgaatg tcaaactcat ctgctagacg attagctact
     1261 gtttctggtg acttagtcgt gttattggta acaaacaaga aaggtaattt tcttttttgc
     1321 aattcttcca caaatcgttt accggccggt atgggttgtg tcccacgata aatcgttccg
     1381 tctaaatcaa ttaaatatcc tttatagtcc attcatccac tactcttccc tttttctgat
     1441 cgtaaatgct gtttttgttt tttctacttg tttaggttgt tcttgttttt ttactttttc
     1501 agaggttttt gctgactctg tttttcgatt tttaataacc ggttttttgc gattagatgc
     1561 gactggtcct tttttctcat cgatatacgc acgattatgg ttttttttct tttttcggcg
     1621 ctgagttgtc ttttcttttt taccgccgat tctctcgatg acaaaatacg cacaaccaaa
     1681 attgcaatat tcgtaaagat agtcttccaa ggaatcgatt cgctgatctg gatgcgcccg
     1741 tcgattatcc gcttggaaaa atccttttag gcgtagttgt tcataacccc aatctcctac
     1801 gatataatca tatcgggcta gtacatcact gtaacgttct ccaagctttt ctgcattaaa
     1861 tccttcacga taatcggaca caagccgata atgtctttca cctatcaaaa aattactgtc
     1921 atctaacagt gtcactagct cgcctttgac tttttctgct ttaggctcct caacgatttc
     1981 ttccaaagca gctgtcagtt cttcggtgac tgttgtttcc tgttcagctg atgatttttt
     2041 cttagcaatt tctgttttat gctcaacgtt ctgctgttca ttttgttttg cagacatgcg
     2101 ttcaccacct atctttttta ttataccttg tttttatttg attgggtagt gagctttcaa
     2161 gtaatctcct accacacttc ggataaattc agggaacaaa aattcatttt ttccagcaat
     2221 ctcaatcgtt ggaaaaaatg gaacaaacat caaatgatct acggtagtaa aagtataatt
     2281 tttattcaga tcgattggct tgttcttcca aaagacttga tggttaatag aatcatatgt
     2341 gattccatta tagaccatct gcccaaaaat cttaccgcga aatcccattc ccttcatagg
     2401 atagttacgt aaaaagttac gatttttttc tatttctaaa actaagcgga tcagatcatt
     2461 tccacccaat gtgacattta acaagtgcat cggatgcggc aacgcagtgt gaagctgatc
     2521 ctgattgacc cgcccttttg gaatttcagt taaaaacaga ccactgttta atattgctgc
     2581 ttccgtcttt cctcgttctt tcaccgcctc aaaggctact tggataaacg aatgctcatt
     2641 gaagatatct agactcagag aaaatggcaa atctgctact tctttttctt ttaataattc
     2701 atgcccacgt tctagataac tatttatttc ttgttcatct tctgtaaaag ctgtcattgt
     2761 ttcggtaggg atggctttgg ctgaatgctt aaggattttt ttctcttcat cgatggttaa
     2821 atggacttca ccgacatact gcccgtactt tccagcggct gctatttgga catcattgat
     2881 tacgtgacct tctctgaata aatgatgggt gtgcgatcct aaaataacat caattgctgg
     2941 tatttctttt gcaatttgga aatcatcctg aatcccgaga tgactcatca aaactaaaac
     3001 atccacttgt gaacgtaaat cttcgactaa ttctggcaaa atatcataag gattccgtat
     3061 gtcccagcca ttcggattat aagtaagcgg aaatggagca gtaaatgcaa ttagtccgat
     3121 tttcgtctgg tgtttcgttg taatgatttt atatggtttc gtccatgccg gtggctggag
     3181 cgtattttta tcgaataagt tatccaggat gatatcaaac tcagcatgat cgtataaatg
     3241 attcaactct tctttggaat ttcctacgcc ttcattgttt cctatcgtcg ctgcatcata
     3301 accaacttca ttcatcagca agatattttc ctgtccattc gttgcttctg acaatggatg
     3361 ccaacgatcc acaaaatcac ctagatctac tgcaatcgtt gtcgtttttt ctttcttttc
     3421 gttttctcgt ttacgttgat ccaaaaacct tctgatcctt ggccaatttt ctaaatgaga
     3481 atgcagatca ttcgtatgta aaatcactat tttttccatg taccctctcg cctctcttta
     3541 tctttatcat accataggca gctttttctt ccattgaaac aagctctatt tcagagtttt
     3601 tcctgccttg aagcaaattg tataagtaca atattcgata aatcagtctg gctaaaaaat
     3661 caggaacatt catttttaca gtactataaa aataaaaaaa acagacccga aagtctgttg
     3721 tcgtttcatt ataatcagac acatggcgga acctgcttag tgctgcttcc ttccagacct
     3781 gacacgcttc acaagcccac cattgtccta gcctttcggc aaagaatagt ataccttatt
     3841 tttcattcgt tgaccagtct ttttttaatt tcttttttcg ctggcggagc tttcggaaaa
     3901 agtcagtcaa aatcatccca cattcgtctt ctaaaatgcc accttctaca tatgcttggt
     3961 ggttaaaccg ctcgtcctcc aaaagattca tcaaggtacc ggctgttccg ccttttggat
     4021 catacgcgcc gaaataaact tctgctacac gagacaaaat catcgcgccg ctgcacatag
     4081 gacaaggctc taaggtaaca aaaagtgcag catcttccaa gcgccagctt cccacttttt
     4141 cacaggcttc cttgattgca agcatttctg catgtgtagt agcatcttgc gtggtttctc
     4201 taaggttata tcctcttcca atcacttttc catccaatac aaccactgct ccaatcggaa
     4261 cttcatgaag catttctgct ttctttgctt ctgccaatgc aaggcgcatc catttttctt
     4321 tttctactgc atcaagattt gtatttacca attacccttc cctgcctttg ttttttcttg
     4381 ttttacttta gcatatgata aaaaggctgt aactaacttt tttttctatg gtaaaataaa
     4441 tacaagaggt gaacaagatg aaaatggata aattacttgc tctttaccca caagcacaga
     4501 aaaaaaactc tccagcatca gaagcggata tcctttcttt tgcaattgat gatgctttta
     4561 tctggatcaa acaagattca ttatctcaac aagaaaccag ccttttgaaa gcgttattcc
     4621 ctgtaatcaa cgatcaaaaa aaacacccat ggtatcgcta tctttttaaa gatgcttctt
     4681 gtgttcaaga aaaaagcttt cgtttgatcc agcttcacgt cgaatcaaaa ggagaattct
     4741 taaaaaaaga atggcaagat actattttgg aattatttcg ctattctgct gatttctttt
     4801 tttatacaga cacagatgcg ctcttagtcg aacaaataaa ccgtgattat cttgatgcta
     4861 ctgaaatcaa cggtattttt ctatctttag atgctgattt tgatacgaca acgtctgtat
     4921 tcgttggttc tttccattca cctggcaccg acctcgtccc tttgtttgct gaagaaagaa
     4981 aaattttttt aacagagcaa aacaatcttt atactcacag ccgtactttt tctatgcaag
     5041 atgtggcttt acattactat acaaaagata ctgtaaaaga tagtaagttg atcaaaagtt
     5101 acatagaact gatcaaacaa aatgaaatgg aagtgatcat tcaagaatta tggaatgaat
     5161 taggcaatgt cagctctgct gcaaaaacac tgtttcttca tcgtaatact ctaaaatatc
     5221 gaatcgaaaa atttcaagaa cagtctggtt tcaatctaaa aaaagccaac gatttactgt
     5281 tttgctatct tttattgctt catgaacata attaaagata gattaaaggg gttgtgacaa
     5341 taattttgtc acaacccctt atttcgaatg aatagtgttc aaaagctagt ttctcttaca
     5401 tcattttttc aacaaaatgc attagccggt cttttacatc ttcggttgaa tctttgattg
     5461 aatcaccata aacagagatt ttcttttttc ctgatttgat ttttgctaac attcccatca
     5521 aagaatccaa gtcactatct gaaagaccat ctacgatttc tagtaatttt tcatttccat
     5581 caaatttgtc atagacgaat ttttttactt tcgtccgagt agtcatatga tggatctttt
     5641 ccattacttt tcccgataca acgactgctg tcgctacact ggcagcggct gctacgctta
     5701 atccgattgt tacttttgtt gatgttttca taagtatcag ctccttttta tctatctcca
     5761 tcatagcata gacaaaaaaa taaacgaact aatccactca gtgaaatctc aatcctttag
     5821 ggaaaaagtt ctttactgtt ccttttactt gtttcccaaa tccgacaggt atcttatcta
     5881 atactagcaa aacccatcct tgattcccat cacgttgaaa tgtttctcct gctgtgtaag
     5941 attgccattc cttttgagtg atcggcaagc aaggtatatt ctctatcttt tttgtagcaa
     6001 gcgctaatgc atagctaggt tcaaatcgat tctttttgaa atctcctaaa tgcaaacctg
     6061 tacgtacgac ttttaaacca tcgagagaag gggctaactc aggaacttcc caaagatggt
     6121 cgttgaacac taaaagtctt ccagtagctt cataatgaaa atcatttgaa aactccgtcc
     6181 agagtttttc ctgctctttt gtcatttgca ctttactttt ttttcgtgtc ttcttttctt
     6241 tatgcatctg attttgtcca tgaaaagtca gctttgcaac aaaatgtcct tcaccctgat
     6301 ctttatgagg ccatatacgt attgtttttt ccaaaccagc tacgcttccc cattcagaac
     6361 gtcctgaact aacggattga gtcaaaggaa tctcctcaat cgttactgga taattttcta
     6421 caagccagct aatgatctct tcattttctt ctggtgcaaa cgtacaagtc gaataaatca
     6481 gttgtccttt attttttagc atttttattg ctgaagacaa aatctcttgc tgacgttttt
     6541 gacagtatag cggagattct tctgtccact cttttattgc atttggatct tttcggaaca
     6601 ttccttctcc tgaacaagga gcatcaacca cgatccgatc aaaaaagccc gaaaaatgag
     6661 ggacaagttc tgcgggtgca tgatttgtca ctattgcgtt cgaaactccc catcgttcta
     6721 tattttctga taaaatcttc gcacgtttcg gaaagatctc attcgtcact aataaccctt
     6781 tccccttcat ttgagcagct aattgggttg atttaccacc gggagccgca cacaaatcca
     6841 aaactttttc accgggtttt gctgcagcag ctgttccaac gatcattgca cttggttctt
     6901 ggctatattc atacccagct tgatggagga aagactttcc attgacagtg ccaagaaatc
     6961 cctcattgct gtagggagcg ggttgtaatt cttcgctatg gtaagtttgg accatgtcca
     7021 atcctgccgg ttttagtgga ttccaccgaa atcctttttt cacgcttcct tgttcaagcg
     7081 cagaaaaaaa atcggatgct tcttcgccta aaagaagacg atattttttg ataaattgct
     7141 gtggtaacgt tgtcgcttcc tccttcattc caatgtttcg ttagacattt tacaatcttt
     7201 tttacgaaaa ataaaggtgg ctgatctact ttttcatgtt gctgcctaag gtgactgttc
     7261 ctttaagata ttttccaagt attcatccgt cacctcgttt ttatcccaaa cagcgttcat
     7321 catttcaaga aaatcttgag aagaatcatc tgagcgcaaa cttcccaatg cattaagcca
     7381 taccaattca agcgactttt cttctttgat cttcgtgtga cctttgacat atgagaaaaa
     7441 tggccgctta aatagcgaat ccatgtctaa atcagatttt gcaatgattt cacgaatact
     7501 ttcatggata gaaaaaaaat ctttcaataa gtagacaatg cttaaaacat catcggaact
     7561 gcattcagct aagtagtttt caaaataaac agtgatttct tctaatatct tctcttttaa
     7621 cccgctgatt gatttgaaat gtgtataaat cgggtatgta gaacaaccgc agaactctgc
     7681 taactctctt gcagtaattg acttcattcc atattcttcc acgtatacca tacttttgaa
     7741 cagcaaatat tctttactat aatttgttac tctccccatc attcttacct ccgtctcttt
     7801 tcatctaccc ataaataaat aacataaata attctaaaaa tattattgat aattatcaaa
     7861 acatatttag ttatatccat taaaaatcaa tttttccgcc ttaaaaacaa gaatatcttg
     7921 acaaaatacc ctaggaaata aacaatagaa taaacaaggt aacaaaaaaa ttaaataacg
     7981 ttatataaaa aagaaggcga cactctgtga gaataacaca gactgaaatg actttccact
     8041 cacagttttt gtctaactat atacaaaaac agaataaaaa ggaggtattt atgctaattg
     8101 aagcaagaaa aaaagagtta cttgttctac aaatggtcat tcaaaaaaat aattgttctt
     8161 tacaagaatt atccaatgac ttacatattc caaaacggac aatcaaagaa ctgatccgaa
     8221 aaataaatgt atcactcaag caatttttat tgattgatga atttatttat tctaatcata
     8281 aaggagagat ccaaaccaaa gaaaccgatc aaactaaaaa gttagctgtt ttttctaagt
     8341 taaaattatg ctacctaaaa gaatccaatc gttttaacta tttgatgtta ctaatcaatt
     8401 atccacaaac tgctgtacca aaaaaatatt tacttgaaca actttatata tcgccctcct
     8461 atctcgaaaa actgacaaaa cagctcaatc attccttaaa aaaattccat ttacggatcc
     8521 tatctgtaaa aaattgctgt actcttgaag gaaatgagtt atctatccgt ctctatcttt
     8581 acttttttct ttcagataca tttggaggta acgattggcc attttcctca cttgaattaa
     8641 atgcactaaa aatgaacagt acagatgagc aaatagaaaa aatttcaaaa caagaaaaaa
     8701 atatctactt gctgttaact ttattcatat tacgaaacaa acaacaggca tattttccaa
     8761 aaacttcttc tgaaatcact gaattgatgg acttattgca gcatacacat gattattcag
     8821 aatactttac ttctttttca gtgagtcgcc cttgtgaaga gacagaaaca gagagagcct
     8881 actttaatta ttttttctat tgtttttttc caaatcaaat ctcttttcga caaaaacagg
     8941 agattggttt tttattttcc acaagcaaaa accttatttg tcgacaagtc ggtcaattga
     9001 tgacttactt aactcaacag tttcctctca accatttaga aaataaaata ccaatttatt
     9061 tttattttct tgtgttggca gttgtttttt tcgaactgtt gggtgaatcc agcagtagtt
     9121 tttctacact ttgctttcca gaagccacgt ccaatcattc tagttctttt atacaggaaa
     9181 agaaattaag agaaaaaatc gatacaattt tttcaaaaaa tggggaaaga aaccaaatca
     9241 gtcaatacct tagtcaattg atccaacact tactacaaac agaaacgaaa gagcagttac
     9301 tcatccattt gcagataacc aaacagttta caggaaatta tcatcttcaa agccatttgc
     9361 agcagttatt tcgcaaagat gctgttaaaa ttaccgcata ttcagaacat gctgatttga
     9421 tcattacaga tcagcatgag aaaatttcag atgaaacgga gactttctat ctggatacaa
     9481 cgcacaatac agtgttattt ggcaagttaa taaaagcggt tcatgaactg tatatgaaaa
     9541 aaggttatga gaacaaaaca aagaggatgt gacaaaattc ttgtcacatc ctcttatt
//
LOCUS       DASJOX010000096         9506 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJOX010000096 DASJOX010000000
VERSION     DASJOX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.097"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..294
                     /locus_tag="U4A12_002156"
     CDS             100..294
                     /locus_tag="U4A12_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774502.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            563..862
                     /locus_tag="U4A12_002157"
     CDS             563..862
                     /locus_tag="U4A12_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774503.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(959..1393)
                     /locus_tag="U4A12_002158"
     CDS             complement(959..1393)
                     /locus_tag="U4A12_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774504.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1625..2335)
                     /locus_tag="U4A12_002159"
     CDS             complement(1625..2335)
                     /locus_tag="U4A12_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1774505.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2325..3185)
                     /locus_tag="U4A12_002160"
     CDS             complement(2325..3185)
                     /locus_tag="U4A12_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN1774506.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3190..3828)
                     /locus_tag="U4A12_002161"
     CDS             complement(3190..3828)
                     /locus_tag="U4A12_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN1774507.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3843..4142)
                     /locus_tag="U4A12_002162"
     CDS             complement(3843..4142)
                     /locus_tag="U4A12_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774508.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4185..5669)
                     /locus_tag="U4A12_002163"
     CDS             complement(4185..5669)
                     /locus_tag="U4A12_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1774509.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5689..6150)
                     /locus_tag="U4A12_002164"
     CDS             complement(5689..6150)
                     /locus_tag="U4A12_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1774510.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="U4A12_002165"
     CDS             complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="U4A12_002165"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN1774511.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7453..8223
                     /locus_tag="U4A12_002166"
     CDS             7453..8223
                     /locus_tag="U4A12_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1774512.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8496..9323)
                     /locus_tag="U4A12_002167"
     CDS             complement(8496..9323)
                     /locus_tag="U4A12_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1774513.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2957 a         1943 c         1488 g         3118 t
ORIGIN      
        1 ttgattcttt ttatttatca taaagatata gatacataat cattttcact tacatatatt
       61 tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt agaagtaaaa
      121 acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata tcctttactt
      181 ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat aaagaacgag
      241 ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa gtaaatccaa
      301 tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga ttatagaatt
      361 agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat accatatgat
      421 tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga tgaatcaaat
      481 atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt tataatgtat
      541 cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg aactacttaa
      601 gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca aagtgacaga
      661 gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg caaaaggaat
      721 atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg ctttaaaaaa
      781 aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat ttgataaaga
      841 ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat actaagtcta
      901 tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt attatatgtt
      961 atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa gaaataccac
     1021 ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt ttctcagtat
     1081 catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc aaagcttcat
     1141 tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg atatcttgaa
     1201 taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc ttcccttccc
     1261 cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt gcagtgtctt
     1321 ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg atcataaggg
     1381 aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata atacagttct
     1441 ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat tccttaaaat
     1501 tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga taaattttct
     1561 tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc ttaccctagc
     1621 atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt ttatctagta
     1681 aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg gccatcagac
     1741 aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc ccccatgtga
     1801 acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat tttctttcct
     1861 taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt acttcttctt
     1921 tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga gtcgtgccat
     1981 aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa tgagtatgag
     2041 taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg agatctgaag
     2101 atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc accatttgat
     2161 ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact cctagctcac
     2221 ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc ggcaatgcca
     2281 gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa gacatcatag
     2341 ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc tcttcgaaag
     2401 tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc tagacgtttc
     2461 aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac atcacgaaac
     2521 tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc agtgatataa
     2581 tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga caaattgcct
     2641 aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc taaaaatttt
     2701 gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat gccatattta
     2761 gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga attcattgat
     2821 ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct tagattatgc
     2881 gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc tggatcagca
     2941 gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg tacaccggag
     3001 ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa tcgtgctaac
     3061 cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc ttttacaagg
     3121 gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat cccaatttga
     3181 gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg cggccttcat
     3241 cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat cgatcccttc
     3301 aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa agcctagttc
     3361 aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag ataacagcgc
     3421 atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat ccatggcttg
     3481 ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt tttgtgctgc
     3541 ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc ctgcttttgc
     3601 aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga ctagtttttt
     3661 atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta agcaaagaat
     3721 tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc ctaatgcatg
     3781 ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct tagttccttc
     3841 ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat ttctttgact
     3901 gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg agtaaacata
     3961 ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact ttttccctct
     4021 gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat tgctttttcc
     4081 acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac taaaattctc
     4141 atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac tctctgcttt
     4201 tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt tttccgatat
     4261 tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc ataacctagg
     4321 actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat cccgatataa
     4381 ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc tacttgtatg
     4441 attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt attggcataa
     4501 actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac gatcgttggt
     4561 gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc aaacaagaac
     4621 ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc aattcctgga
     4681 acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag ttcatcgacg
     4741 aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa agaagtcgtc
     4801 ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat caaataatct
     4861 ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga agtagcaacc
     4921 atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat ttttttgtct
     4981 tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc taattttgca
     5041 aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc ttctgtcaat
     5101 tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc taaaatcagt
     5161 cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag taaaatccaa
     5221 aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga acgcagtttc
     5281 gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag taataaaatc
     5341 atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac agcattttgt
     5401 ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag tccaaccaag
     5461 attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa gtaaccgacc
     5521 gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa aatatagcct
     5581 aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt tgcaaaatac
     5641 gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt aattatattc
     5701 tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct caaccaatcc
     5761 atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt ctggatcttt
     5821 tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt cactttcaaa
     5881 acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac catcactttt
     5941 ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac cgtatttttc
     6001 tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat cgatcaatac
     6061 aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg ggtaatgaat
     6121 caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta taaaaacgac
     6181 ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata aaaattaaac
     6241 tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca gatataaggt
     6301 ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat ctcttttgga
     6361 tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt tgcattcaat
     6421 gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc tcgtgggttt
     6481 tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt ggcaaataaa
     6541 ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc gaataaatag
     6601 tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc ttctggatct
     6661 tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt gatgtctttg
     6721 attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat tccccatttt
     6781 tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca attttgatga
     6841 actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac gattaaagcg
     6901 tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa attaggctgc
     6961 atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat gccattccca
     7021 tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc ttcatgactt
     7081 ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg atccactact
     7141 tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc tactaaccac
     7201 ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc atatcgttgt
     7261 tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt ttatatgacc
     7321 gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat aataaagaca
     7381 gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga tgtccctcag
     7441 gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa agaaattttg
     7501 gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc ccttgatgta
     7561 tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga gatcatacgc
     7621 aattacggtt attgttctta taattacaaa aaccagactg attttgatca atctggtcct
     7681 gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga ttatgatacg
     7741 ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca atctaaccat
     7801 ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga taattgtagt
     7861 tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg cgacatcgca
     7921 ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag cggagtcgat
     7981 gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga aatgatggtc
     8041 aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg actgacatca
     8101 aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa attttgttct
     8161 gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt agattcattt
     8221 taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa caaatttatc
     8281 gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg tatccatcca
     8341 atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc taaataggaa
     8401 tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt tcgaataaac
     8461 ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt ttcaatcacc
     8521 tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat atatctttct
     8581 aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag caagtattcc
     8641 aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca taattggatt
     8701 gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata tacaatgcag
     8761 accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc cacataaact
     8821 ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc agctgcttgt
     8881 aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata aaataatgct
     8941 gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa aatttgattt
     9001 aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt ataagattct
     9061 tggtattttg atacagaatt caatgattga atctcattta tttttttcct gattttgtct
     9121 tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca aagcgcactt
     9181 aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt tgcttgttca
     9241 caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta ttcttttagt
     9301 aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta tttctccttt
     9361 atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga ataaaaattt
     9421 taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga tttctttttg
     9481 tgttatctga ataattatcg tttaca
//
LOCUS       DASJOX010000097         9368 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJOX010000097 DASJOX010000000
VERSION     DASJOX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.098"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..404)
                     /locus_tag="U4A12_002168"
     CDS             complement(<1..404)
                     /locus_tag="U4A12_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1774514.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLN"
     gene            complement(394..2568)
                     /locus_tag="U4A12_002169"
     CDS             complement(394..2568)
                     /locus_tag="U4A12_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEN1774515.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2584..4926)
                     /locus_tag="U4A12_002170"
     CDS             complement(2584..4926)
                     /locus_tag="U4A12_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774516.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4948..7491)
                     /locus_tag="U4A12_002171"
     CDS             complement(4948..7491)
                     /locus_tag="U4A12_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1774517.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7501..7899)
                     /locus_tag="U4A12_002172"
     CDS             complement(7501..7899)
                     /locus_tag="U4A12_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1774518.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7980..8264)
                     /locus_tag="U4A12_002173"
     CDS             complement(7980..8264)
                     /locus_tag="U4A12_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774519.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8288..9286)
                     /locus_tag="U4A12_002174"
     CDS             complement(8288..9286)
                     /locus_tag="U4A12_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1774520.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2664 a         2005 c         1465 g         3234 t
ORIGIN      
        1 tgattcaatg tcttttcaaa aaagaatgtt tttctctcaa tttttttgac gtttctatca
       61 tgaatcacga gaaaacttgg gacactttta acatggtaac gttgtaaaaa agtttgaagt
      121 tcttcttgtc ttggttcttt tcttgtttca attcgatata cattcctaga gaattttttg
      181 tcgtatcttt ccaagaaaga ttcaacttct tggcattttt tacaatcatt tcttgctaga
      241 tacacaaaaa atgattgatt cgttttttga agttctagta cttcttctaa attactattg
      301 attagttttc catttgattc ttcgttagtt tttatcagtt taaaacaacc aaataagagg
      361 aaactaacaa taagtatcct aacaataact ttcttatttc gcatattgtt tctccaaatt
      421 tggtttccga atatagacga ctcgcggtgg ttcctggacg tcttcagctg ttcgatacac
      481 cacaaattcg cctgttctca atgctcgaat gcgattcgga ttgaatttaa agcggtctac
      541 gtctcgtttc gtgcctcttt ctcctgtcca atcaaccttt cccatatcag aataacctgc
      601 ttcttgtgtc gtcatttccg ttaattcaat gtcttcttct gtccccatta aatctgccca
      661 ataatcaatt tctgtcgggt catttgtctt cccaataaag aacgtattca cattcccaat
      721 caactgtttt cgaaggacag gatcaattct atcaatgtcc gcaagcgttt gaggggcata
      781 cgccgcatgc accccagcgc ctcgtccctt attgactaag tctataaggt tatcatttag
      841 ataagaagct ggttcgtcaa atattactag gactttcgag cgattggctg tttctcctgc
      901 ttgtttatac acttctgacg caaagaaatt aatatctccc acaagcattt gagccaatgt
      961 ccgaataaac tcactataaa tcaatccgtt taacgagata taaacaatct ttccttcttt
     1021 aacaacttct tgaatatcta attctctttt tcctccagaa gtatcaaaga gttcaccaag
     1081 ctcactatcc aataacatat tgatattggt ctgtaaattc tcagaagtcg catacaaata
     1141 aaacgggttc ttcttatgtt cgtaacgaac aaacaaccgt ttaaacaacg tttgctcgat
     1201 ttctgtcatg agagaaatgt tttcttttaa taatagataa tacgtttcta agtcaacggt
     1261 tttacgatca tgttcaacga ttcgctcttc acttaactgt tccacatcgg caaaatcacg
     1321 ttctgttttg tccatcgctt cattcttttt cgtttttttc ttcgtggttt tcttcttttc
     1381 tacgaccact ttttctttaa ataaatctgg atttttcaac tgaatacgat ccgcaaacaa
     1441 atgtaagacg tttcgtggca ataaataatg ttgtaaccaa actagatttc tttcaatttt
     1501 atggctcttt tggaactcgt ctaaaagctg aatcgttacc tgtaataacg ctttggctgc
     1561 tgtcgaataa tacacgcttt ctgtttctgc tagattggtt aatttatctc gaatggcaat
     1621 atcattgcca ttttttacag gattataacg caaatcgcca ccgtccacaa agacttcaat
     1681 cgggcgattg tatgcctctg ccacttcttt taacgcttgt aaagtctctc ctgctccttt
     1741 cccatcgact aagataacgg ggatacgaaa cttacacgca tgttctgcta gtaattggat
     1801 caccgtcgtt tttcctgaac ctgtagtcgc tggaataatc acatgttggt tcaattcact
     1861 aaagttcatc acataaggga gcccatactc atctgttcct aatagaatca ctttggctaa
     1921 tgaagccagt tgtttattcc gcaaagcaac gtcttttatg acaattgcta cctggtacgc
     1981 ttgctgatac tctgctgtat atttttggga ctcctgaaat ttattcttcc gacgttcctt
     2041 gtattcttta ctatttagca aagctgtttg ccgttctttc tttgaaacaa cttttctttt
     2101 ttcaataaaa gacgcatacg caatcaaaca acttcctgca cctgtcccac ctaaaaggag
     2161 ggcaaaaatc gtttgtccat taaactgcaa cgcatgagaa aacaagttta atggaaataa
     2221 taagtaacac gacaaatacc ctctaggaag aacaaaaata aaacaaaata gatttaatag
     2281 cgtaaaacct aacgcaaaaa taaaaaaaag agagcttctt cgttttaaga agctctcata
     2341 ctcaaagtca taaatatgtg cacgtgtctc ttttttatct atcataaaga caatcgctaa
     2401 aggaatactg cctacaataa aaaatggata tagacaaaca cctaataaag tgacgataaa
     2461 ataaagttgt tgaaaccgac tttttggcgt ttctttttct tgttttcttt tgcgatcgac
     2521 agaaaacatt tgctcaaaat gtgtcggtcg ttttggtgca ttcatcatac tatcgatttc
     2581 ctcctatcgt tttcgttgtt tcatttctct tcgataaatc ggttgttttt ctcgttttcg
     2641 tttcattttt gcttgagttg ctcgaaactc actccgtgtc ttcggtgctt gttttggtga
     2701 ttttgtttgt ttcatggttg agtatgcttt agatacggtt gaaggcgctt tggtttgtgc
     2761 tgttactcga ttgactgctg acatatgttc ccgaaccccc tctggacgtc gaacagattt
     2821 cggtgtggta gaacctgttg ctcgtggtac attccctttt cttagtggtt tttgtaccgc
     2881 atgtttttgt cctgcaactg tccgggtttt catcgaactt gtttttgtta cgggagtaag
     2941 tgcctccttc cgacgacgac ttgtttgtcc ctgacgacgt tttcgatcgt tcaattctgt
     3001 tctaggcgta tgcttctggc cataagactt gtgtttttgc tttggattcg ctaatgacac
     3061 caccattgca gaaccctgat tgcgatacgg actacgagac gcaccttttc tacttctttc
     3121 tcttagacta ggattgcctt gcaattctcg ttgtaataaa tcggctgttt ttcttgcctg
     3181 tttctccatt gctgttcgtt gcatacgttc ttgactttgc tcttctgctt gtttcgcttg
     3241 atcatgcaag tctcttgctt tttgtgccat acgttccata cgatttggtg ctttatgacg
     3301 aggttgttgt gcggattcac gccgaaaagc acttcgtgtg gcttgtttcg ccgtttgtgg
     3361 catacgaacg tctgagccac ccccacgttt tcgttgacgt tcttgtctct tccgtgcgtt
     3421 ttctttttct ttttgtttct gtgcttttct cgcttctttg cgttctttca accgttgttt
     3481 ggcttccctt ctcaattttg cttctgcccc aaatggattt cccgcaaaag ctaacgctcc
     3541 tcgaacaccc attcttccag taaatagact gcctaaaaag cctcggaaca aataaattcc
     3601 caacggcgca aacaaataaa caatgatcaa gagaattttt tcccatggat cgtcttgtgt
     3661 actacttaat tggaacccgt aactaagaaa agaagaaaaa aggatacatg caaaacctgt
     3721 cattcctttt aaaaacgtca aaataccaaa aactttggca tagttaccaa acacattcat
     3781 tgtgacaaga aacaatccta aaaaaagtaa gaacggcaac atcggtacta acaatagttg
     3841 catagttcca ataatcaatc gaaacatact taagacaaaa taaacaacgg tttgaatcag
     3901 attcacgaca aaataaaaca aggcaaatgc tgtatttttc caactattgt aatattgtat
     3961 gttcttgttt ttcagtgtat ctgcttcata ctctgtgact ttgtcatgaa ttttctcccc
     4021 tatttttgta cctaaatcat tgtccaataa aagattgatt cgatcatatt cttttccagc
     4081 atatttgact ttcttctcac gaattgtgtc tacattggtt gtgccataat ttaacaacag
     4141 atatggttca taaacattac tataaaagac ccgagccgcc atcaatttgc ctgccgtttc
     4201 cactttttta tccgctgtta cctcttctgt ttcttttgtt tcataatttg tttcttcttc
     4261 aggtgttaac actggattga ccttcacgaa cgccgtttcc actgtagaat ctaaagaaaa
     4321 aagaacatca aaaagattct tatttgtccc agaatctttt aatacgctta acccagtaaa
     4381 aataaggatc gtcataagaa agacacggag tgcttgtttc attcgagcct ctccagcata
     4441 tttgattgcc atcataattg ccgaaaaact gattccaata gttcctgcaa tacttaacat
     4501 attgtccgca attttttgtg ttaagttttg aatgggttct cgaatggcgg aaataatatc
     4561 taattcaaag agaaattcaa ccattgaagc attgaattct cctaacattt ttacgccact
     4621 ccaaacaaaa tttttcatca ctcctgcaac attgtacgat agcttttttt taaggttcat
     4681 tgactcatag tcattggtca tcaattcaaa cgaattcttt ttgtaatttt tatacaattg
     4741 atccacatca gtcgttactt cttttacttc tactttatcg ttggcattct ctccttcaga
     4801 acgatcggtt tcagtgacac ccgcaccatc attcatttga tcggcagaaa cagtagcccc
     4861 ttgtagacag aaaaaaagac cccctaataa aaggagcgtc caccatttca gagatttttt
     4921 tcgcaaaaaa accacctctt ttttgtttta tcgttgtttt tcattttcta acattgcttg
     4981 aattttttca tcttgatttt cagtagaaga aacagccaat aaccaactat caaacaacac
     5041 atcaatggca atcggttgat ttcttcccaa atgatcctgg aataaacagg ttcctgtttg
     5101 taattgttcc atcatttcta aattggctga attcacttcc atatcaaaga aggacaatag
     5161 tttctctcgt gcttcttttt gtttcggacg aaacgcaaat ttgtaagaaa ttaactcttt
     5221 tttatcttct tgatcgtaat ctttaaacgc ttgcacaaca aggtaaatat ccgtattatt
     5281 cgcacgtcct tttcgcaagc tatcatcaat caaatagctt ccttgtgcgg tatcttcata
     5341 cccttttgcc tcgtcataaa taatcgcagc gtcttcttct ttgtttgtag aaaagatata
     5401 cgtaaacttc ataatgactt ccataattgc cattcctgca agttgttcgc tatttaatcg
     5461 acccgaatca atctcttttt gtgtcggaat gcgtattcct tgtgtcccta acacgttgat
     5521 ttgtgaatca aaacgtatcg gcgtactgtg ctcattccca atgagaatct ttcctaaacc
     5581 aacgctatgc cctaaaatca atttagctag ttctttgtca ctttttcgaa tttcttcgat
     5641 aacttgactt aacgaatgct tacctggcat ggtgtaaact acatcgatgg cgtcttggat
     5701 caccgttttc ttgtggctcg ctgtttctcg attggtatct acttctccta acattcctaa
     5761 cacattttta gcggtttgaa ttgcctcttc ttttggtaaa aacaatagag gatcaagcat
     5821 ccctcgacag ctttcatctg aagataacga aataaaatta attcgttcat acatagccac
     5881 aaactctggt atgtgtccga atttttcaag tgcctttaaa aagaactttt gggtttcatt
     5941 tttcggatca atataaagaa ttttttgacc taaaaacatc agccataaaa aaatatattt
     6001 tacaaggacc gattttcctt tacctggtgg accaataatc aacgtatttc cattggtatg
     6061 ttccgcgcct tcaatacttt tctttgttaa attgggataa taccatacta gtttactaga
     6121 tagcgctaac gcttctttcg cagatttaaa cttttttgca ataacaatcc gtcctaaagg
     6181 aaatcctttc ttatttccta cttctttctt taagtccaag ccataatctg ccacaaaacc
     6241 tgtcgtagcg acctgctcgt aactacgaaa acgttgcgta cttccaatta agcattgatt
     6301 aaacaacgtt agctgatccg ctaccgatcg ataaaatttg aaatctgtag actcaatcgc
     6361 aaattcaata tctttgactc gttcttccaa ttcttctttt gaagaagcag atacaaccag
     6421 cgtgacggta gaataacaca atcgtttttc tttgcgcttc agcttatctt ttacatccat
     6481 taaccgttct gtaccaaaat ttaatacatc gtctgaatct aaaatgccgt caacggtctc
     6541 atattctttt tcttgttcat aaatccgtcc agccatttta ttaatttttc gttcatcaga
     6601 ttttgaatgc tcaaatcgaa gtcgaatatg cgtatcaatt ggaaaagata agctatcctg
     6661 aagattttga ataaagctac tcccatacat ggctaaagga gcgtccgttt gacttaaaaa
     6721 cgtcgaataa tgcgtttgat tcaactgttc aatcgcaatg tatcctgctt cattcgaaat
     6781 gacgccctcg caaatttcca ttggcgtaag ctgtctttca ggcaattttt cttctgatcg
     6841 atggaaaaaa taataataaa tttgtccaat ctcttgggca gataagcgct ctatcggctt
     6901 ataattggac aattccgtat agagctcctt ttctaatgtt tcatacgttg gtaacacact
     6961 acttatagga actcgttgtc ccgtaatacg atagataaac tttttaaaaa catgcttaag
     7021 caattgaaaa tattcgatcg gatcagacac tttcatttct gtagtgaaac gcacaaacag
     7081 atacgttcga tactcgttta attgcacttc gtcttttaaa atctttcctg cctgttcaaa
     7141 gtacactttc ccaagatctg cgcattctcc ctttaccaaa tctcgaatgg tttcttcaat
     7201 gtgcgtatct aggttaaatt gacgtggaat attgataaaa tgatagtcat attcatcatg
     7261 tccaagtatg ccttcgccat cttgaagata gttcttgaaa aaatcaatgt catttaaagg
     7321 aaatacttgg gaagaaagtt gatagcctac ccacacatca tcatttttgg tcaaaacaaa
     7381 atttcggata atggcgtcta tcggaaattc taatcgagga agcttcgttt ttttcttttt
     7441 gaactgccaa atcccgataa taaagacgac caaaagaaac aaaaacgcca tttactcgac
     7501 tcactccttc catttgtgta gggaatacgt cactttttca tggaaaaacg gcacatacat
     7561 ttcatgttca aagcgttgtt tccgtttctg ataataggac gttcgcccaa ttaaatactc
     7621 gaaaaagtgt ttattgtccc atttcattga aaacaatgtc caaacgagaa caccaatccc
     7681 aaaaatcata atcaaccagg actttcgaaa cagtgtctgc aagaaactaa tttgattcac
     7741 atacgcaatc aaaaatagga tcaaaagaat gaagagaaat accccaccta tgactaattg
     7801 ttccatgcga atgccattag aaatccaggg aattttttgt cctttaatcc cgtaaatttt
     7861 gtgagggtgc ttaaactcct ttgtatagtt gtattccatg ttcactctac ctccttttac
     7921 aaaaaaagaa gcgaccagaa gttctaatct tctgatcgct tttgcagttt gtctttttct
     7981 tatgataatt gtatccaggg cacaatcatt tgcgttaccc ccacgttctc ttcactatcc
     8041 ttaataaaga taatgttctt tagccattca ccaatatcaa cggcattcga taacaataag
     8101 acgagcatta tcaaaataaa gacgccaata atggcaaacg ctgcccgcgc aaagccttgg
     8161 gtcgcataag caccaaccat tagaacaatc gcaacgattg ccaatagcca aatgacttgt
     8221 ccagatacat catcaaataa cgatttaaga tcaaaaagtt tcatttccat tctcctctaa
     8281 gttcatttta ttcatttaac gtagtaacaa aataactatc gccacttgtt tttacttcca
     8341 ttgaaaagtt tgattggaac tcgtttcctt tctcaaaata gaaacgatac ttcccttgca
     8401 aaaagtagtt tccttcttta ttttttactg catgatccag ttgtacattg atcaattcgc
     8461 cgccttctaa ccctttttcg ttagaaatca atgccaattt ttcatcattt ttgacatata
     8521 actcaaaaaa ccgtttcaca aatgcttcca gcttgatttc ttcgctttca gtgaccgctt
     8581 gcccagaaga ataaaatcga ttttcctgat agttcacaga attggtattc ttactctcag
     8641 aaagattcaa aaattgaacg ccatcaagta cttttaaaac attgttttga taactgactg
     8701 gaaaagaaag ttgactgctt ttccaatgtt ctccttctgt aaaagtaacc tcataaaata
     8761 ggcgatacat tgcttgtgtc aaatcgattt cttctagttt aataaagcga acatctttcg
     8821 ctactcgatt ggtagccgtc gttacaaagc ctagatcaga cgcttccaat ccttgtgcta
     8881 aataatgatt gacggaagcc tcccattcgt ttcgtttttc tggcgaataa gtaaataacc
     8941 tttcaataaa atgttgtccc tcgtacttca attgttccgt agaagacaag gtttggttga
     9001 cttcagttaa caatgtttct ggattgactt gttttttcac tgctaaacgc gttaatgtat
     9061 ccactcgtcc aaagtttagg aggagcacga cacccattaa cagaaacaaa caaaaaaaag
     9121 cgcctgaaaa tacactcatg gcactttttt gcgaaaattg gatcattcga tctgacggtt
     9181 ctgttttctt tttttggaac aaaaaacgtt tcttttgttt cttttccttc tgttgtttcg
     9241 gtggcttagt ttggaacgct tgtggattaa attttgcatt actcatgaga aatgccatac
     9301 cgttctaaca ataatttcgg tattttgtcc acaatcacta cttcgtggac aaccacactt
     9361 gccccctc
//
LOCUS       DASJOX010000098         9361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJOX010000098 DASJOX010000000
VERSION     DASJOX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.099"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(92..760)
                     /locus_tag="U4A12_002175"
     CDS             complement(92..760)
                     /locus_tag="U4A12_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1774521.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="U4A12_002176"
     CDS             894..1181
                     /locus_tag="U4A12_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774522.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="U4A12_002177"
     CDS             1597..3135
                     /locus_tag="U4A12_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN1774523.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="U4A12_002178"
     CDS             3132..4097
                     /locus_tag="U4A12_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774524.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="U4A12_002179"
     CDS             4084..5406
                     /locus_tag="U4A12_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1774525.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="U4A12_002180"
     CDS             5647..6390
                     /locus_tag="U4A12_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774526.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="U4A12_002181"
     CDS             6405..7397
                     /locus_tag="U4A12_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1774527.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="U4A12_002182"
     CDS             7409..8146
                     /locus_tag="U4A12_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774528.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="U4A12_002183"
     CDS             8136..8858
                     /locus_tag="U4A12_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1774529.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="U4A12_002184"
     CDS             complement(8942..>9361)
                     /locus_tag="U4A12_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1774530.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DASJOX010000099         9358 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJOX010000099 DASJOX010000000
VERSION     DASJOX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.100"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="U4A12_002185"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="U4A12_002185"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN1774531.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="U4A12_002186"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="U4A12_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN1774532.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="U4A12_002187"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="U4A12_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN1774533.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="U4A12_002188"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="U4A12_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN1774534.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="U4A12_002189"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="U4A12_002189"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN1774535.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="U4A12_002190"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="U4A12_002190"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN1774536.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="U4A12_002191"
     CDS             complement(8053..8523)
                     /locus_tag="U4A12_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN1774537.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="U4A12_002192"
     CDS             complement(8569..8994)
                     /locus_tag="U4A12_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN1774538.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="U4A12_002193"
     CDS             complement(9061..9330)
                     /locus_tag="U4A12_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN1774539.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3287 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta attttttt
//
LOCUS       DASJOX010000100         9281 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJOX010000100 DASJOX010000000
VERSION     DASJOX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.101"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="U4A12_002194"
     CDS             <1..435
                     /locus_tag="U4A12_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1774540.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="U4A12_002195"
     tRNA            complement(759..842)
                     /locus_tag="U4A12_002195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="U4A12_002196"
     CDS             1370..1870
                     /locus_tag="U4A12_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774541.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="U4A12_002197"
     CDS             complement(2060..2404)
                     /locus_tag="U4A12_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN1774542.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="U4A12_002198"
     CDS             complement(2558..3478)
                     /locus_tag="U4A12_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1774543.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="U4A12_002199"
     CDS             3665..4156
                     /locus_tag="U4A12_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN1774544.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="U4A12_002200"
     CDS             complement(4180..6555)
                     /locus_tag="U4A12_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN1774545.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="U4A12_002201"
     CDS             6881..7507
                     /locus_tag="U4A12_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1774546.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="U4A12_002202"
     CDS             7530..8234
                     /locus_tag="U4A12_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1774547.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="U4A12_002203"
     CDS             8426..9259
                     /locus_tag="U4A12_002203"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN1774548.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DASJOX010000101         9152 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJOX010000101 DASJOX010000000
VERSION     DASJOX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.102"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1872
                     /locus_tag="U4A12_002204"
     CDS             <1..1872
                     /locus_tag="U4A12_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1774549.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="U4A12_002205"
     CDS             complement(3807..4472)
                     /locus_tag="U4A12_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774550.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4652..6634
                     /locus_tag="U4A12_002206"
     CDS             4652..6634
                     /locus_tag="U4A12_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN1774551.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="U4A12_002207"
     CDS             6638..7276
                     /locus_tag="U4A12_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774552.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="U4A12_002208"
     CDS             7304..7732
                     /locus_tag="U4A12_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774553.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..9100
                     /locus_tag="U4A12_002209"
     CDS             8189..9100
                     /locus_tag="U4A12_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1774554.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         3187 a         1399 c         1723 g         2843 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     9121 gaactattta aatcaataaa actgttcatc ta
//
LOCUS       DASJOX010000102         8863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJOX010000102 DASJOX010000000
VERSION     DASJOX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.103"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="U4A12_002210"
     CDS             278..787
                     /locus_tag="U4A12_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1774555.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U4A12_002211"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U4A12_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1774556.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U4A12_002212"
     CDS             2322..3302
                     /locus_tag="U4A12_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1774557.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U4A12_002213"
     CDS             3424..4842
                     /locus_tag="U4A12_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN1774558.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U4A12_002214"
     CDS             4913..6382
                     /locus_tag="U4A12_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1774559.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="U4A12_002215"
     CDS             6392..6859
                     /locus_tag="U4A12_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1774560.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="U4A12_002216"
     CDS             6895..7767
                     /locus_tag="U4A12_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1774561.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8863
                     /locus_tag="U4A12_002217"
     CDS             7848..>8863
                     /locus_tag="U4A12_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1774562.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEI"
BASE COUNT         3047 a         1322 c         1788 g         2706 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tag
//
LOCUS       DASJOX010000103         8851 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJOX010000103 DASJOX010000000
VERSION     DASJOX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.104"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..730)
                     /locus_tag="U4A12_002218"
     CDS             complement(<1..730)
                     /locus_tag="U4A12_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1774563.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFL"
     gene            complement(736..1842)
                     /locus_tag="U4A12_002219"
     CDS             complement(736..1842)
                     /locus_tag="U4A12_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1774564.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1842..2978)
                     /locus_tag="U4A12_002220"
     CDS             complement(1842..2978)
                     /locus_tag="U4A12_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1774565.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3010..4461)
                     /locus_tag="U4A12_002221"
     CDS             complement(3010..4461)
                     /locus_tag="U4A12_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1774566.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4505..5269)
                     /locus_tag="U4A12_002222"
     CDS             complement(4505..5269)
                     /locus_tag="U4A12_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN1774567.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5297..5995)
                     /locus_tag="U4A12_002223"
     CDS             complement(5297..5995)
                     /locus_tag="U4A12_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1774568.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6007..6786)
                     /locus_tag="U4A12_002224"
     CDS             complement(6007..6786)
                     /locus_tag="U4A12_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1774569.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6802..7746)
                     /locus_tag="U4A12_002225"
     CDS             complement(6802..7746)
                     /locus_tag="U4A12_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1774570.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7792..8571)
                     /locus_tag="U4A12_002226"
     CDS             complement(7792..8571)
                     /locus_tag="U4A12_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774571.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2522 a         1838 c         1316 g         3175 t
ORIGIN      
        1 ttaaaaaaaa gtctctaatt atagcaaaca tccatctaga ctcttgatta catgctaatg
       61 aataacccgc aaaatatcct gatgctacgg atgcgtgagc ataatcaggc ctcctaatag
      121 accaaatatc gatatcaaaa tactcatctg gtattggttt tgtacaataa aatgtggcgt
      181 ctaaccacaa acctccatgt tttgctagca aagataatct taataaatca gaataatttg
      241 ttctcgtaat gattcctttg tttttctttt cttccaccca ttttggtata tcaacatatt
      301 gtctataatt atcttctgtt aaaattataa cagtatgttt ccctgcattt cgttttattg
      361 attttacaca agcttttaca atttccggcg attgatccaa tccctgccac caacaaaccc
      421 aaatgcagtt agaatatggt gattttgatt gattaattat ttgattttga ctataatcat
      481 atgttttcaa aaaatctgaa aaagtctttt cataatactt cagcattacc tcatgttttt
      541 ttaataaatg ttccttctgt tttttacttt cattatatcc attacgattc atgacttgaa
      601 tatcaatttt agcttgtaac gtggcaaatg cttccttcca agatgttatc tttgcaattc
      661 gtcttgttgc attcaattct tcggatatgc gatgtataat tctttttata cttgaatgtt
      721 tctgtgtcat attctttaac ctctttgtat taattcaaat aactcataat attttgattc
      781 taagattttg tatgctgttt gaatattata atctgccttt attaaaatcg aacgttctga
      841 atctcgatga gagatatcta catttgcaat ctctctcgcc cattcttttg ccgattgatt
      901 taaacttatg aaattggtct tatcactgaa agcaacttct gtaggaactt tctccgaaaa
      961 gaaacacggt aaatctgcaa attgagcttc aatacccact aacggaaccc cttcaaataa
     1021 tgatggcaac attaatacgt caaatgcttg ataaaatttt tctatatcat ttctatttcc
     1081 taaaaattta atatcatcat ctatgccact tttctttgca taatttttta tttcttcttc
     1141 catatcacca gttccaataa ttaataatac ggaattattc ttaatagcgt gaaattcttt
     1201 aaaaatttca atcaaaaaac gatgattttt agaatatgaa attcttccaa tatgtcccat
     1261 tacaaacgtc tgacttgaaa ttcctaattt cattcgtaca tcatttctaa tactttcatc
     1321 ataataaaat tttttagcat ccactgcatt tggaatcaaa gtgaatttat catttcgaaa
     1381 caaaaaatca cctgctactt ttccacatga aaattcatgt gtcaaaacag acgttaactt
     1441 acatcgataa tattgcttta aaagatattt aaaatctcta tctatcgatg tattatggct
     1501 atgtgctatc cgtatcggaa tcccagcttt ttttgcagct tttaaaggta aataactcat
     1561 cgcatcaata tgagaatgaa caatctcata ttctggatga tccttaaaaa attgttccat
     1621 ccatttaaaa tatttaggat aattttgtgg ataaagtctc ggtgcataat agacctttcc
     1681 tccaagactt attatttcct catcataatc acttttatat ggacgatgtg tcaaaaaatc
     1741 aaactgaatt ttagttcgat ctatattcct ataatagttc attagcatcg tctccaaccc
     1801 tgcacgatgc atatcattca cacactgtaa tattcgaatc attatatatt tcccctatac
     1861 aaatttaatg tttcttgtac gattttatcc caattatatt tactacaaat aaactcagta
     1921 gcttgattct tcaaaacttt aaccatttcc gattgattgc atgcctcttc taatcttatc
     1981 tttaaatcac ttacatccga tttcttaaaa atcatagcct tgtcttctac tacttccgta
     2041 cactctgaga tatttgaaac tatacaacag ttcccataac tcattgcttc taacaaactt
     2101 aatggcattc cttctaaatc acttggaagt gtatagatat acgcattact atataattct
     2161 tctaattcct gtccttgtac aaatccagta aatataattc gctcatcacc ttttgctaat
     2221 tcctttagtt catttgcaaa ttcatctgta tcagagcttc ctccggctat tataagtttt
     2281 ttatctgtct gaacatcttt aaatgcctca attagatacc gaattccttt ttcgggaacc
     2341 aatcgcccca aaaacaaaat ataagaatct tttgttaatc catacttttc tgtaatcaat
     2401 cctgctgatt ttatttcaat ttttttaacg ccatttggta taaaccttgt ttttcttcca
     2461 tatatatctt caaaataatt ttggacgctt tcactaagta caattatttc atctgcaaat
     2521 tttacagcac acttttcccc taacatgatg tatgtactag caagcttgtt ccatttttct
     2581 ctttgatgat ctagaccatg aattgttgca acacatcttt tcccaaaaag ttttggcaac
     2641 cacaacatag cacatggtcc ttctgcatga aaatgaacaa catcatattt tccgaatgct
     2701 gctctaattc caccaaatac tgaagaactc attgctgcaa ttccttttat atttaatgta
     2761 aaaatagtct ttaattttac acctttatat tctttatttt tatgctggtc aaattccttt
     2821 ccgctaacat gatgaccact tcgattatag caagtcactt catgcccaag tgcaaccatt
     2881 cgcgttgcca attcttctac cactatctca attccacctt ctcttgatgg aattctttta
     2941 tgacctagca tacagatttt taattttttt cttgccatca cgttttactc cttctatagt
     3001 atctttatct tacatggaac cgtttctatg aagcacagtt ttgactgtcc taaacaaaat
     3061 tctaaaatct aatcccatac tccaattcct gatatattct gcatcaagtt ttacaacctc
     3121 ttcgaagtct gtgatatcac ttctaccact cacctgccac attccagtaa tcccaggttt
     3181 catcgcaatt ctcgcacgat gatgaaaatc atacttctca tattctgact tcaagcaagg
     3241 tctaaacccc accaggctca tttttcccat taggacatta taaaattgtg gaaactcatc
     3301 caatgatgta gatcgaataa attgtccaat tcctgtcttc tttgttccat caggaagtat
     3361 tttattgcca atgattcttg ggtcaaaatc catcttaaac atgagattat tactcatctt
     3421 attttctttc atcaactctg ctttgcgttc ttctgcatcc atatacatac tacggaactt
     3481 atacatctta aatgtcttac cattttgacc aatacgttct tgtgcaaaga aaatcggccc
     3541 tggtgatttg atgtaaataa ctggtgctac gaagataaaa ataacacctg ttgcaataca
     3601 tccaataatt ccagcaagaa tgtcaatcgt tctctttata aatgcttgcc tcatcgtcat
     3661 aacattaata ctagatgtaa taacagtata atcaccaact cggccaataa attgttttcc
     3721 acgtgtacta tcagacactt ttgtaagatt catatgtaca gcaagtccca tttccgaaca
     3781 tctggcgatt aattctttgg gatatggaac actttcgtca acattgatga atacttcatc
     3841 tacccaattc tgtaaaagat attccgctgc attatcagca tttgcaacta ctgggacacc
     3901 tccgatggta gttccataca tatctctgtc tactacaatc acaccactaa gtttatacat
     3961 gccataattc ttctctttaa gattacgaac tacatttttt gcaatctttt gagatgttac
     4021 aataactaac gatacatctt caccgttttt catgcggtga tgcaacaacc ctttccaaag
     4081 tatacgaatt atatatgtta ataatccata caccaccccc attataaata aagaaactct
     4141 ggaataatca tttccatcct gcattgtaat aaggtataaa ctgcacacaa gtaacagcat
     4201 aaaagattgc tttatagaag ctacagcctc tttataatat cctcttttta aaacattttt
     4261 atatgcctca aataaaaata taatcgtaag attctcaagt tctataaaaa ttgccatgtg
     4321 gcgataaata ggaatcacat acggattcca gtcaccctgt cgcattacat atgatatata
     4381 aaaagcaagc tggacacaaa ataagtctaa taaaataaaa tcaatatgtt tagaccaacc
     4441 acttgagttt tttctataca tctctcactc tctcctcatt tcctttttat ctacgaataa
     4501 agaactaaaa taaaccaaac ttcttctttt ttacctcttc atagattgga taattgatct
     4561 catccccgtt gatcagatcc tttgcgactt gtttcatctg tactactttt tccttaccaa
     4621 actccttggc aatctgctca tagcactctt ttaagtaaaa ggtgcgctta gagaccccgt
     4681 gtgcatcaga agcagccatc tgcacgagat tatgtttgac cattgttttg gctgttttct
     4741 ggatatcttt gccaaataca ccaacataac ttggcgcagt aagttgggcc aaacatccca
     4801 tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa atgcgcgttg cgttctggat
     4861 ggacgatcac tggtatcttt cctagttcta acaactcaaa aaaaagccgt tctgcatata
     4921 acggcacttc cagtgtcgga aattcgatca gaagatatgt atcatctaaa tctgtaaata
     4981 aaatttcatc tcggtggatg tcttcgatga gcgttccggt aatacgtact tcttgtcctt
     5041 ccaataacgt caaaggcaac tgtcttttct ctaattcggc ttgtaacgaa gcgactagag
     5101 aaatgacttg tgatttttca ttgctgtatt ttccattatt gtggtgtggt gtgcataaaa
     5161 tatgcgtaat gccttgctga atcgcttttt cagccattgc aatacttgct tctaagtttt
     5221 ctgccccgtc atcgattcct ggtaggatat ggcaatgtag atcgatcatt tctcttgtct
     5281 cctctcttct tattccttaa tttccgtagt aataaccagc atctttgctg tgttctgctc
     5341 cgttgtatac gacacctaag actcgtgctt gtaccatatc taacagttct tttgctttag
     5401 tcaatgattc ttttcgtgac gtattttccc gtacgaccaa taaggtgcca tccgtttttg
     5461 aagacatgat ctgtgcatct gttacggcaa cgactggcgg catgtcgaag atcactacat
     5521 caaatagttg tcttgcttct gctaaaatct gatccattct tggcgaactt agtagttctg
     5581 atggatttgg tggtttaggt ccacttggca gaatcgacaa attgtcgact ggtgttcgtt
     5641 ggatctcatc tgctacactt cctgatgaac taagaacagt acttaatccg cttgcgttgt
     5701 tcaacttgaa cgttttatag atgacgggct tacgtaagtc tgcatcgact agcagcaccc
     5761 gttgaccaga tttggcaaaa acaacggcaa tgtttgctgc agtggtcgat ttcccttctc
     5821 caggtcctga tgaagtcaca acaatcgttt tgatttgctg accagctgcg gaggcaaact
     5881 ggatattcgt ccgaatcgta cgatattgtt cagcgattgg tgaagaggga ttggtgagtg
     5941 tgaccaaact gactgcatga gtttgggctg ctttttgttt ctgtgttcgt gccattttct
     6001 ttcctcctag actcttgagc ggcttctgcg tgatggcgct gttttatcgt ttgtagcttt
     6061 tggttgtgtc tgagctggtc cttttttacg gataccagca ttcaattctt tcgctgacat
     6121 ttctggaact gttcctaaga ttgggaatcc taatgtatct gtcacatatt tttcatcttt
     6181 cactgtccga tcgagtaatt ccaataagaa tgccagacct acacctacca tcattcctaa
     6241 cattaagcca gtcattaaat tcaatttatt atttggcgaa acaggtgtcg tattggcaac
     6301 tgcacctgaa atgatcgaga ttttatcaac attcatcaca tcttttgcgt tctcttggaa
     6361 aacttgtgaa gttgtgttag cgatctgttg agcagccact gcattcgtac ttattgcctg
     6421 aatcgagaac atttgtgaat tttctgcctg attaactgag atggcatctt tgatttgttc
     6481 agcagtcatt ttcacattgt cttctgtttc taaacgatct ttgacttcat tgagcactaa
     6541 atcactgacg atcatatctt tatacgtatt gatcatttgc aggttcgtat tgacatcatt
     6601 gacatttgca gtgtctgatt gtggcagtgt cacgatcaat tgtgcttgtg aactgtattt
     6661 tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc aagccagcaa acattgagat
     6721 gatgattatt accaagtgtt tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc
     6781 ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt ttaattcttt
     6841 tttcacacga tcaagttctt gatcactgac tttttgatag gagatattat cttgcatgaa
     6901 gccttctcct tgcagctgat cttgcttaat cgtaccaaat gcctcccgat aatctaaagc
     6961 aatttttttc atttcatcaa agttcatgtc tgttttcata ttcgactcta gagcattcaa
     7021 aacggattga tagttgctga caccgtttag actcaatact tttttcgcta cgccttctac
     7081 gatctttctt tggcgccctt gtctaccgta atcaccattt gggtcttcat aacgcatacg
     7141 agaataagat aatgcctgat ccccgttcaa tgtaatttta ccgattggaa agtcatagcc
     7201 atcttgtgag aaagtgaggt cattattgac ttcgatacca ccaactgcat caacgagttc
     7261 ctttagccct ttcatattga tagagacata gtgatcgatt gggatgtcta catatttttg
     7321 tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt
     7381 tgtgtcacga cctacgatgt cgacatacgt atcacgagga atgctgacga tcgttgtctg
     7441 tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt gatcgacccg
     7501 acctaaatct ccagtatcaa tccccatcaa tagaacagag aaagattctt gtttgtccag
     7561 atcaactggt ttcgttcgtt ctccttgggt tcgttccact gattcgtaag ttttctcaat
     7621 agaatgtgag agatccagat ataattttgc tcctactcct acaattgcta atactaatac
     7681 gataagaact gctaaaataa tcagaaatat ttttttccct cttgattttc ttcttcgtct
     7741 tgccatagtt tcctccgttt gctaaccttg cttgaatcaa cgttttaact gtcaatagct
     7801 tatattgcta tagcccattt gctcagcaac agtttgtgct tttgttgcgt atgctgcgct
     7861 tattttttca ttccggatct gctctatttg actgagcacc gtcaaatatt gatcgtaagt
     7921 agcggtggca gtcacttgac catcctttaa catcgtatcg aataactgat ctagttgcgt
     7981 gacttgcgcg atctgaatat cagcgaatcc tagatattgc ataacaaccg ttttccaagg
     8041 actatcttta aaaaagctca tattttcacg tacacgagcg acatctgcac ttgtcacctt
     8101 ttctttgatg atgacatcgt ctacagcttg ctgccagttt gatacatttt tagtaaataa
     8161 atcgttgaca gctgtctgga tcttcagttt tgattctgca ctatctaact gtgtcacgat
     8221 tgctttcttc tcatcttgga ttgcaacagc ttctttaggc aattctgatt ctttgatgtc
     8281 gtaatcatct gctgaaagtt tgacagagga tagcttattt gacaaacttg tgattgtttc
     8341 ttctgttatt tcagggttta gaaaaatttt ttcttttgta ctatacagct tgtctacttc
     8401 ttttgataaa gaagtcaagg tgtctttttg attggtgatg gcagattctg cataattgat
     8461 ccgttgctga tgttgccgct gattattgat atggtatcca aacaaacaga tcgcaatagc
     8521 acctgcacct accgccacag cagtcaatga cttgacgaat ggcttttcca tagattcact
     8581 cctaagtatt gatgatagat aaattaagat ataattcact tacataaaaa tgacagcttt
     8641 ttatttaaaa aaacataatt aaactaatat attcacagaa atatttgtaa acaagttttc
     8701 cctatataat cataccacaa actgttttct tgactagggc aatttacgat ttaaaagtaa
     8761 ataaaacatt aataagataa aaattaatcg atttcatttt tatttccata attttcatag
     8821 gtgtttttaa aagttacaca aaaaatagaa t
//
LOCUS       DASJOX010000104         8784 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJOX010000104 DASJOX010000000
VERSION     DASJOX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.105"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..2857)
                     /gene="rnr"
                     /locus_tag="U4A12_002227"
                     /pseudo
     CDS             complement(14..2857)
                     /gene="rnr"
                     /locus_tag="U4A12_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(2860..3615)
                     /locus_tag="U4A12_002228"
     CDS             complement(2860..3615)
                     /locus_tag="U4A12_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1774572.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(3718..3954)
                     /gene="secG"
                     /locus_tag="U4A12_002229"
     CDS             complement(3718..3954)
                     /gene="secG"
                     /locus_tag="U4A12_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN1774573.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(4072..4350)
                     /locus_tag="U4A12_002230"
     CDS             complement(4072..4350)
                     /locus_tag="U4A12_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1774574.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(4463..5431)
                     /locus_tag="U4A12_002231"
     CDS             complement(4463..5431)
                     /locus_tag="U4A12_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1774575.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      5646..5690
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5714..6688
                     /locus_tag="U4A12_002232"
     CDS             5714..6688
                     /locus_tag="U4A12_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN1774576.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            6693..7268
                     /locus_tag="U4A12_002233"
     CDS             6693..7268
                     /locus_tag="U4A12_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1774577.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(7335..8474)
                     /locus_tag="U4A12_002234"
     CDS             complement(7335..8474)
                     /locus_tag="U4A12_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN1774578.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         2344 a         1892 c         1572 g         2976 t
ORIGIN      
        1 aaggataatg aaattatcta gagaaatttt ttaatgttcg atcaatttgt cgatcgatat
       61 cttttcgttt caaatcttcc cgtttatcat aagatttttt ccctttcgcc agaccaatca
      121 ataccttggc atagccgtta cgaatataga cttttaaagg aacaacagtg attccagtat
      181 ttttcaattc attttcaagc cggatgattt gttttttgtg taataataac tttctcgtgc
      241 gcaacggatc atgattaaaa atatttcctt gttcataagg actgatatga acattatgca
      301 agaaagcttc tccgttgcgg acgcgaataa atccatcttt tagattgatc cggctgtttc
      361 gtatcgactt gatctcggtt ccttgcaaga ccatccctgc ttccatcgtg tcgatgatcg
      421 aataatcatg gcgagctttc ttgttttgtg caattaattt tccctcgcct tttggcatag
      481 ctgtttcctc caactcattt tattttttct ttttcttctt tttaacacct ttgtaaaatg
      541 gcttcttccc ttttttctta ccgaacgttt gcttactatc gctatatcgt ttgttcttct
      601 tgccatttct ttctgggcgt tttcctgctt gttttccttc gcgttttcct gattttccat
      661 ttcgtgtttt tggccgttcc aacgctgtaa cctcttctgc ggaaacaagt tcaaaatcta
      721 ctgctcgttc atctggatca gctttgacta cttttactcg cactttctgt ccgattttaa
      781 aagtcagacc agttctttca cctacaagag ctaaatgatt ttcaacaaaa tggaagtagt
      841 cctgtttcaa ttcattgata tggatcaatc cctctactgt attcggcagt tcgataaaca
      901 ggccgaattt cacaacagag ctgatgatcc catcgaattc ttcccccact ttgtccatca
      961 tgaattctgc tttcttcatg gaatctactt cacgttctgt ttctactgca cgacgttcca
     1021 tcttagaact atgatctgca atttcaggaa gttccgtagc ccacgaactt tgattgcttt
     1081 cacttttatc ttcactgtaa gaacggatca gacgatggac gattagatcg ggatagcgtc
     1141 gaataggaga tgtaaagtgt gtgtagtaat ctgccgctaa gccataatgc ccaaaattgt
     1201 cttctgaata gcgtgcttgt tgcatgcttc ttagcaacat agtattgata actgcagctt
     1261 ctggtttgtc ttctactcct tcgatcacat gctgtaaatc ttttggtgtg atcgttcctt
     1321 tcgttcctcg gaccagaatg cccaaagctg aagcaaaatc aaagaagcgc tgcatttttt
     1381 cttcttttgg ctgttcatgg attcggtaaa taaacggcag atttaattta ttataatgtt
     1441 cagctaccgt ttcattggcc gcaagcatga aagattcgat caagcgttca ccaattcctc
     1501 tgcttcttag ctggatatca tttgggtgtc cttcagcatc aacaaggatt tttgcttcgc
     1561 gatcctcaaa agaaatcgcg ccacgtttga ttcgcatcgt ttcaaggatt cgatgcaatt
     1621 cacccatttc ttggaacata ggtacgagct gagcatagcg ttgcatcgtt tcttcgtctt
     1681 gatcttctaa gatttgattg acagctgtgt aggtcatccg ttctgttgtt tggatcacac
     1741 ttgggaaaat atcatgagag actacttgtc catttggatc aatctccatt tcgcaactca
     1801 tggtcagcct tggaacatgg ggattcagtg aacagatacc attcgataaa cgttgcggga
     1861 tcatcggaat cactcgatca gtcaaatata cacttgtccc tctttcatat gcttctctgt
     1921 ccaattgact gccttgtgtc acatagtaag aaacatctgc aatgtgtact ccaagaaaat
     1981 aattaccgtt atctaatttt ttaaccgtaa ctgcatcatc tagatctttt gcatcttctc
     2041 catcaatcgt aacaatgagc tgatttctta aatctctacg tccagttata tccttttcgc
     2101 tgatttgatc tggaacttca tctgcttcag acaatacatc atcaggaaat ttcgtaggga
     2161 taccattagc cacgacaatt gagaggatat ccatgccagg gtcattctta tgaccaatca
     2221 cttgcttgat tatcccttct aaactagtcg catacccttt ttcagggtaa tgggtcagtt
     2281 cgatcagcac aatacttccg tcaactggac gaactccctc tgctgctgca tatactttaa
     2341 attggttgag ctttctatct ttcgggataa caacgccgta aagatccgtt tcttgtattt
     2401 cttcttctga ataagcaacg aattcccctg ctaactgagt agtcgcccgg tctcgaatct
     2461 cgactacttt cccctctgct ccgcgatcca tatctgtatt tgctcgcttc atgatatcga
     2521 ttgctacggt gtctccatcc attgcaaaac cagtcgcttc ttttgggata tagatgtcat
     2581 cttcttcggg atcaatcgtt acaaatccaa atcctcgttc atttgctcga aatgttcctt
     2641 cgatcaagat tggttttaaa ggaagtttca cttttccttt tttattgaaa actatactct
     2701 gttctctttc catttgagca atcgtttgta ctagtaattt aaaatcatcg cttttttgga
     2761 accctaaacc ttcagcgatt tcttccatag aaaaactgcg tttgcgactg ctttccatga
     2821 aaaagagaat tttctctttt atcgtttctt ttgtcatatt tattcctcat tccaaggtaa
     2881 tttttctaaa aattccaaga cgtctttttc taattcgcgt cttgctgtac caactgtaat
     2941 cacatgacca ctttcaggat accatcgaag tgtcactcgc tgacggctca acttgcgtgc
     3001 agtttcaaat acgccattag ggtcgatcat ttcatctttc ccagcttgag ctaaaaatat
     3061 cggctgttgg atatctgaca acctactttc tacgattgct gcctgatctt gtatatccat
     3121 taattgttgt tcagctaacg agcggtaagc aactgctttt tcattgattt ctttctctga
     3181 tttacctgct gtcttcagca cttgacgtac gtatttttca aagttttcag gcacgtttgt
     3241 ttttacagga gacaaaggcg aacagaaaaa accgccgcca atcactgatt cttcttctag
     3301 tgctcgtaca gcaaagatgc cgcccattga taatcctaat acggcaatat cagaaaactg
     3361 ctcagactgt aaaaaatgga tggctttttt agtgtctgcc caccagcttt ctgctttttc
     3421 ttgcaaaata tcatatggct ccattgttcc gtgtccttta aaaagcggcg cataaactgt
     3481 ataatctgct ttttccaaaa atcgtgcgag cattcgaaca tcgtttggac ttcctgagta
     3541 agcgtgaagc agcaaaaccg ctcgctttcc atgttttgca taaaggggtt ttggtaattg
     3601 tctcactcgt ctcacctctt tcgcctcatt ttaccatagt ttctttcata acttcttctt
     3661 aagcgttctc tttgcaataa aaaaagccgc ccgccgtttt gcgagaggct tattcaacta
     3721 cttcgaagat aaatatgcta ggacaaataa taagatcatc cagatagctc ccataactgc
     3781 agttgatcgc tgcatgacag cttcaaagcc gcgagctttt tgtttgccga atagttgatc
     3841 cgcaccgccg gtaaatgcac tcgctgcact attttgcttg cttggctgca tcataatcgc
     3901 aatgaccata acaatcgata aaacgatcac gattcccaaa attaaattat acatatcgca
     3961 gcctccttcc caaaagatat cgtctcttta actttatcat agataaaagt ccgtgacaac
     4021 tctatataaa tggaataaca gctgaaagtt caaaactttc agctgttatt cttatgactg
     4081 acgtaagacg tcttgatatt ctggtttctt ttcaaactct ttttttgcat aagggcataa
     4141 aggaatgatc tttttcccgt ctcgtcttgc taattctact ccagtataca ctagcttttc
     4201 agctaacttt tgaccacgat atgctggatc tacaaatgta tggtcaatga tcatgatgtc
     4261 tgggcccgca tctgaccatg tcatttctcc gatctcttgt tgttcatcat tgaataaagc
     4321 aagacgattt ttttcttctt tgatttccat atgccgcact cctcaaataa ctgatatttt
     4381 tttattccta ccttatttta gcagtctcct gatctataaa caattattta tcctcgaatt
     4441 acttacaaaa aagaagagaa aattaattca tcgttattct ttgttcaata aatgaaaaaa
     4501 tcactgtcca ataggttttc ggatctttca tcgatgcttc caagtgttct gcttctggaa
     4561 caagcagcag tgctttatcc cctgccgtag cattttgaat cgtgtacgct gcttctgtag
     4621 ggacaaaatg atctctttca ccatgaatga ccaataaagg cagatggttg ctgccaagct
     4681 gtctcgtagc tgatgcttgc tttaaggagt aaccggcgta tttctgggca taatgattcg
     4741 catatctcat gaccattttt ttaggaaaga cagtgatttt acttaacata tatcggaatt
     4801 ctgcataaac agaaacatat ccgctgtcta aaataaatcc tttgaccgct gatggaagtt
     4861 tttcaccact tgccatcatg atcgtagaag ctcccattga tccgccgtgt aaaataatgg
     4921 acgcattcgg ctgttcatct aagatgagtt ggatccaggc aattaaatct agccgatcta
     4981 gccatcccat tccgatgatt tctccttcac tttctccgtg cgcacgtaaa tcaggtacaa
     5041 gaacgttgaa tcctttctct gcatatcttt tacctatatg agacatgtct cttttccctg
     5101 tgcttcggta atcatgtacg caaatgaccc atttattctg cttatttgga tggatgaaca
     5161 tctgaccagc tagtttcaac ccgtcataac ttttgattgt tttgggtgtt ccctgctccc
     5221 aaaagctttc acactcttct tctaattttt gtcgtttttt ttctactgac gtgactgcag
     5281 cagggtccat caaacgatga ccagtgtctt cataccagtc atatcctctc tgtactcccg
     5341 ctcttactaa tttctttgca taaaaataag aaagaaaatg gtaactgatc cctataatta
     5401 tcagaataag aatgaaataa aaaataacca ttgtacaccc gccgctttct gccaaactat
     5461 aatctaacta ttatccaatg taccgatcca taggattctt tattttcggc acacgtaaat
     5521 tccatttctt tttcattgta tcatttactt aaagttctcc tcaatagatt tacttgtaag
     5581 agagcattta cattcgcaat aaaagccagt atactaattt cgtgcattat aatcaagacg
     5641 ctcgtcttgg actggttcgg aaacttccca gaataaaaaa ctaagtatct ctaaagaaag
     5701 gagattggaa ataatgcgaa aagtaattat tgattgcgat ccagggatcg acgacacttt
     5761 agctttatca ttagctgtca aatcccctga tatcgaagta gttgctatta ccgtagtttg
     5821 tgggaatgtc cctgcagata taggtacgca gaatgtgctg aaatgcctgg aacgctgtga
     5881 tcgtttagac attcctgttt atcaaggaat ggaaaaacct ttgaagcaac ctttcatcag
     5941 tgcacaagac actcatggta tggatggttt aggggaaaca aatttcccga tgatcttgag
     6001 aaaacaggca gaacctctcc atgccgttga ttttttggct gattatttca aagaaaagac
     6061 aaatacatca gtcattgctt tgggtccttt gaccaatatc gcttttgctt tgaaagtcaa
     6121 cccaaatatt gggaaacata tggatcgttt tgtctcgatg ggtggaactt acaaaagcca
     6181 tggtaactgt tcgccagttg ctgaatacaa ttactggtgt gatcctgaag cagcttctta
     6241 tgtattcgag aatctcaaac aaacgataga aatggttggg ttagatgtga caagagaaat
     6301 cgtcttgaca ccgactatcc tcgaatactg ctgtcaaatg aatcctgaag agggagagta
     6361 tctaaaagcg atcactcgtt tttattttga cttccactgg aaacaagaaa gaattttagg
     6421 atgtgtcatc aatgatccgt tagctgttgc ttattttatc gagggggcca tctgtgaagg
     6481 tttcaaaagc ttcactgctg tggaaactca aggaataagt cgtggacaaa cattggttga
     6541 tcgttatgaa ttttggcaga aacctgctaa cagtaaaatt atgacaaaag tagatactcg
     6601 tctttttttc cgaaagtttt taaccgttct tttaaatgca caagaagaaa cgattatgaa
     6661 agatttggaa aggttaaaga tgggctaaca aaatgaataa aaacaaaatg gcaactcgcg
     6721 tacttacagt gatggctttg tgtattggga tcaattatat tggaggaacg attgctttat
     6781 ggcttcgttt gccaatctat ctcgattcta tcggaacgat ttttgctggt gcattgcttg
     6841 ggcctgtacc tggaatgctg acaggattgt ccagcggtat tttaagtgga gtgactacag
     6901 atattttttc tctgtattat tcgccaatcc aaattctcac aggcttgctt gctggattga
     6961 ttttgccaag atatctcggc aaagataaaa acagcttgaa actgttatta ctcgctttga
     7021 ttttgtcggt acctggaacg atcgtttctt cgatcattac cgttcagtta tttggcggga
     7081 tcacctcctc aggatccagt atgatcgttc agctattaca tgggttaggt ttaagtcaga
     7141 caacaagtgt gatcttggta caggcaggta cagactacct tgatcgtttg ttgtctatcg
     7201 tcgttgtttc attagtcatc actatgttgc cacaacgctt gatatattca aaaagaaaaa
     7261 taggctgaat aaacagagct ggattagcaa aataatgcta atctggctct gttttttcat
     7321 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcaatcata
     7381 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
     7441 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
     7501 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
     7561 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
     7621 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
     7681 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
     7741 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
     7801 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
     7861 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
     7921 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattagtt
     7981 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
     8041 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
     8101 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
     8161 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
     8221 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
     8281 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     8341 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     8401 tcaccttcgt ttccattcac tgatttaatt cggatgatct cttgtaaaat cgcgattttt
     8461 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     8521 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     8581 atatgctgct acttccctcc tattttattt atacgagagg acttgctcat agattcctat
     8641 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     8701 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     8761 aaaaaactct ctacctgctg atat
//
LOCUS       DASJOX010000105         8675 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJOX010000105 DASJOX010000000
VERSION     DASJOX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.106"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1101
                     /locus_tag="U4A12_002235"
     CDS             <1..1101
                     /locus_tag="U4A12_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1774579.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="U4A12_002236"
     CDS             complement(1280..1609)
                     /locus_tag="U4A12_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1774580.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="U4A12_002237"
     CDS             complement(1599..1871)
                     /locus_tag="U4A12_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1774581.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="U4A12_002238"
     CDS             complement(2322..3125)
                     /locus_tag="U4A12_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1774582.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="U4A12_002239"
     CDS             complement(3103..4227)
                     /locus_tag="U4A12_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774583.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="U4A12_002240"
     CDS             complement(4243..5058)
                     /locus_tag="U4A12_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1774584.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="U4A12_002241"
     CDS             complement(5048..5920)
                     /locus_tag="U4A12_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1774585.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="U4A12_002242"
     CDS             complement(6005..7294)
                     /locus_tag="U4A12_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1774586.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="U4A12_002243"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="U4A12_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DASJOX010000106         8572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJOX010000106 DASJOX010000000
VERSION     DASJOX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.107"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="U4A12_002244"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="U4A12_002244"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1774587.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="U4A12_002245"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="U4A12_002245"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN1774588.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="U4A12_002246"
     CDS             complement(3070..4323)
                     /locus_tag="U4A12_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1774589.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="U4A12_002247"
     CDS             complement(4329..5252)
                     /locus_tag="U4A12_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1774590.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="U4A12_002248"
     CDS             complement(5473..7965)
                     /locus_tag="U4A12_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1774591.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="U4A12_002249"
     CDS             complement(7972..8439)
                     /locus_tag="U4A12_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN1774592.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DASJOX010000107         8494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJOX010000107 DASJOX010000000
VERSION     DASJOX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.108"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U4A12_002250"
     CDS             complement(<1..173)
                     /locus_tag="U4A12_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774593.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U4A12_002251"
     CDS             complement(593..1714)
                     /locus_tag="U4A12_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN1774594.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U4A12_002252"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U4A12_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN1774595.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U4A12_002253"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U4A12_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN1774596.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U4A12_002254"
     CDS             complement(4304..5215)
                     /locus_tag="U4A12_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1774597.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U4A12_002255"
     CDS             complement(5232..6239)
                     /locus_tag="U4A12_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1774598.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U4A12_002256"
     CDS             complement(6236..8365)
                     /locus_tag="U4A12_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1774599.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="U4A12_002257"
     CDS             complement(8370..>8494)
                     /locus_tag="U4A12_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1774600.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DASJOX010000108         8237 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJOX010000108 DASJOX010000000
VERSION     DASJOX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.109"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            473..1336
                     /locus_tag="U4A12_002258"
     CDS             473..1336
                     /locus_tag="U4A12_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1774601.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1465..3087)
                     /locus_tag="U4A12_002259"
     CDS             complement(1465..3087)
                     /locus_tag="U4A12_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1774602.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3336..3857
                     /locus_tag="U4A12_002260"
     CDS             3336..3857
                     /locus_tag="U4A12_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1774603.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4043..5773)
                     /gene="spxB"
                     /locus_tag="U4A12_002261"
     CDS             complement(4043..5773)
                     /gene="spxB"
                     /locus_tag="U4A12_002261"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN1774604.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5938..6756
                     /locus_tag="U4A12_002262"
     CDS             5938..6756
                     /locus_tag="U4A12_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN1774605.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6859..7056)
                     /locus_tag="U4A12_002263"
     CDS             complement(6859..7056)
                     /locus_tag="U4A12_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774606.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7225..7803
                     /locus_tag="U4A12_002264"
     CDS             7225..7803
                     /locus_tag="U4A12_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1774607.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7898..8035
                     /locus_tag="U4A12_002265"
     CDS             7898..8035
                     /locus_tag="U4A12_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774608.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2473 a         1480 c         1626 g         2658 t
ORIGIN      
        1 ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
       61 aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct cacaacctct
      121 tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
      181 ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat ttcacaacct
      241 ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt
      301 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
      361 ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga
      421 tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa atgtgtatca
      481 aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag aaaaagttga
      541 ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc tcggaaagac
      601 attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta cgacatcaca
      661 aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac agattcctgt
      721 tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc ttcaagcggt
      781 tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata caaaagctgc
      841 aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag
      901 catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt catgggtaac
      961 agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa gtgctactct
     1021 tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa ggctgacgaa
     1081 tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg agacggtaaa
     1141 aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg atctagagag
     1201 cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg tgaagcttta
     1261 tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga ttttttcgat
     1321 gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa
     1381 caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt cgctgttttt
     1441 ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt tttgaatatc
     1501 tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac ctttatccga
     1561 aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg acgcaaataa
     1621 gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt ctaagtctaa
     1681 atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca tttttgaaag
     1741 catgcaacgg actttctctc caccagataa aacattcact ggtttcaata cttcttcacc
     1801 agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt cttctttact
     1861 tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc
     1921 ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag tatctggcgt
     1981 aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa
     2041 agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt tctttccatc
     2101 aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac gttcaggttt
     2161 gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag taattttttc
     2221 aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac taaagcgggc
     2281 aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag
     2341 tttagccgct agttgactag attccaacca aaaatcataa tttcctacat aaagtttgat
     2401 tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt gacggtcatg
     2461 ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc aattgatcga
     2521 ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt taccaaatag
     2581 tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca tttgctgatg
     2641 gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg gttcagcttc
     2701 ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac catcttcgtc
     2761 agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat acagacgctt
     2821 gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat ggttctgttt
     2881 caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag gctgcagatc
     2941 ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa tcaagccata
     3001 gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt ctgaaaagtg
     3061 caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt attagttttg
     3121 acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc gaacattctt
     3181 tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga aaatagctac
     3241 tataggattt attagtttgc aacagcataa tttttgacgt tatttataag agggagtact
     3301 ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga accatcgact
     3361 ggggctcatt gattttggga attttatttg tacttgtctc attgttatca tttcaagacc
     3421 ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt attaaaggga
     3481 tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa ggaaagatgc
     3541 ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc aatattggtg
     3601 caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat tcagttttag
     3661 cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg tttacgatca
     3721 tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca atttcttctg
     3781 cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc acacatatcg
     3841 tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg attacagttt
     3901 tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga ggtgattttt
     3961 aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat tttatcttaa
     4021 tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc aatactggca
     4081 aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa tattttcttg
     4141 gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc acatcgatta
     4201 ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc gagtattctg
     4261 tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct gcatcttcta
     4321 aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag
     4381 aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt gtaatgatat
     4441 cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt ttttctgggt
     4501 agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg aaccatccag
     4561 atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg atagtcgtgt
     4621 taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt tctttataga
     4681 cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac caatttaccc
     4741 atttttcttt attttcttga tttgctaggt accagctatc agcagggcga gcatccccta
     4801 actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa gatgtttcat
     4861 ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca gttggattaa
     4921 aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg gcttctgcca
     4981 atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag ttttcataat
     5041 catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa aaatgttctg
     5101 agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa agaactggtt
     5161 tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct ggtaatatca
     5221 cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa
     5281 gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct ttaatggctt
     5341 catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat acgctaacat
     5401 ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg gatgtagaag
     5461 caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca tacaaaccat
     5521 tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact ttccccgtta
     5581 attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt
     5641 tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat cctccgggaa
     5701 tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg attcctgcat
     5761 tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt ttttgtatct
     5821 agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa aaatgtagtc
     5881 atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg gaatggcatg
     5941 gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac agcttttact
     6001 tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat caaaaaagaa
     6061 gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag tttttggtct
     6121 ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg gctagttgta
     6181 agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac actgccccat
     6241 atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa acggacgatc
     6301 ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt gggggtagca
     6361 ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc tgtttcggtg
     6421 gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat cccgttgcga
     6481 caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg aatcgttgaa
     6541 ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct gcttccttat
     6601 gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa
     6661 gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat gagtggattt
     6721 atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata tatgatatat
     6781 caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat atcataatga
     6841 agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt tgatccagcg
     6901 aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc ataaaagccc
     6961 gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca ctaaaagaac
     7021 catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc ttgttcattc
     7081 tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg aagactgatt
     7141 tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat catctaaaat
     7201 agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct
     7261 gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa cggagacacg
     7321 tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag ttttatcatt
     7381 ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa gataaatgga
     7441 cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc gtttgcaact
     7501 tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg ttttgttacc
     7561 ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc
     7621 cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct atcgattggt
     7681 ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt gctgatcgca
     7741 aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg
     7801 taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat aaacgatcaa
     7861 atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt
     7921 aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc atttgacttg
     7981 gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa aataaacatc
     8041 tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag tatgattctg
     8101 ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat cgaaaaatag
     8161 atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa attagtcaaa
     8221 gaagggcgta tagattt
//
LOCUS       DASJOX010000109         8196 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJOX010000109 DASJOX010000000
VERSION     DASJOX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.110"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(607..2238)
                     /locus_tag="U4A12_002266"
     CDS             complement(607..2238)
                     /locus_tag="U4A12_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN1774609.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2252..3925)
                     /locus_tag="U4A12_002267"
     CDS             complement(2252..3925)
                     /locus_tag="U4A12_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1774610.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3938..4255)
                     /locus_tag="U4A12_002268"
     CDS             complement(3938..4255)
                     /locus_tag="U4A12_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1774611.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4280..5680)
                     /gene="lacG"
                     /locus_tag="U4A12_002269"
     CDS             complement(4280..5680)
                     /gene="lacG"
                     /locus_tag="U4A12_002269"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1774612.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5917..6702
                     /locus_tag="U4A12_002270"
     CDS             5917..6702
                     /locus_tag="U4A12_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1774613.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6705..7397
                     /locus_tag="U4A12_002271"
     CDS             6705..7397
                     /locus_tag="U4A12_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1774614.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7671..8042)
                     /locus_tag="U4A12_002272"
     CDS             complement(7671..8042)
                     /locus_tag="U4A12_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1774615.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2497 a         1806 c         1411 g         2482 t
ORIGIN      
        1 ttattgcact tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc
       61 ttgatagtat cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag
      121 ccaacataat atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt
      181 gattttcatt aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg
      241 tttcattctt atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt
      301 ctatccatct aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa
      361 acaaacttta tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg
      421 ttgaactctc tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg
      481 taaaaatgac tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt
      541 cctgccttct acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc
      601 cgcagtctat tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc
      661 cttgctataa accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat
      721 ttttgtccat tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt
      781 gatatataac tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta
      841 agttcctttg cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg
      901 ccaaaaagtt agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt
      961 agcggttcct tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct
     1021 ttcgaaactt gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa
     1081 gcttggcaag gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat
     1141 agtggattga acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt
     1201 gtaccaaaga ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat
     1261 ccgatcggca agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc
     1321 cgcagctgct tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg
     1381 cccataagct gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat
     1441 attggtttga agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa
     1501 atcaaatgac acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc
     1561 gccaccatca gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc
     1621 ttggatacca gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca
     1681 agacttgcca taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc
     1741 aggataaacg tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc
     1801 cgtattcatt tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg
     1861 atcaacccaa aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc
     1921 tcgttttgca aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc
     1981 attactatta taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt
     2041 taaaatctct aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc
     2101 catatctgac aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac
     2161 agtttttgaa acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa
     2221 ccaaattccc attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa
     2281 cagctttttc aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct
     2341 tgattcctaa gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg
     2401 ctaagaccac catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca
     2461 ttgcactgat cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg
     2521 cacttgtccc tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga
     2581 taacttgact cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg
     2641 ttgcgatcac ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc
     2701 aaaatggtag atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta
     2761 agatacccat gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa
     2821 cgtacataaa gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta
     2881 caacaggtgt taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa
     2941 atagcaatgg ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct
     3001 tcaactgctt agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag
     3061 ttcctcccat agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt
     3121 caccggaatt gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg
     3181 ccggctccac gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc
     3241 agataatagc gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa
     3301 tgattgcttc tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg
     3361 ccgcaaatac tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag
     3421 gaacttcttt aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa
     3481 ctgttaaaaa tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg
     3541 aaaatccacc ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca
     3601 tcacagaggt ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca
     3661 agcagcgagc agtggttgct gaaacgagta accctacaac acccatcgaa taattataga
     3721 ctttccatag ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg
     3781 cagcaatcaa aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt
     3841 cacgaacggc tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt
     3901 cgatttgttt aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca
     3961 gtacaataaa gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc
     4021 catggataaa aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt
     4081 ttgtttgcga attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa
     4141 catgttcaaa gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt
     4201 cgcctgaata tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt
     4261 cttcctccat tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt
     4321 aagctgattc ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc
     4381 ttttgttata cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct
     4441 tcacatttac cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt
     4501 cgattctcgg ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt
     4561 tctcagtgat atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt
     4621 catatagacc ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat
     4681 aaatacgttc tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt
     4741 tatgatggat tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga
     4801 tccctaagta gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa
     4861 aatgatagct ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg
     4921 aataatagcc taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa
     4981 aggcagcatg cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa
     5041 ctacaccaat ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag
     5101 caaccatcat attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg
     5161 ggaaaacacc taacaaatat tgattggttg caactggata gatttcatta aaggtactcc
     5221 acaccttgac ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta
     5281 ttgtttctcg attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat
     5341 gcaacgtcac aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa
     5401 aagcgattcc ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa
     5461 tggataatct tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt
     5521 gatgataaaa atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat
     5581 cccaagcaac agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg
     5641 tggctccgcc aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct
     5701 ataatcagtt ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca
     5761 acaaaaacaa acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca
     5821 aacataaaaa ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt
     5881 atatgataga ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt
     5941 actatcgtag aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa
     6001 ttagatgttt ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag
     6061 ctgctgcgtg tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct
     6121 cacactgaaa aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc
     6181 tccttgattc acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg
     6241 gcgacgcatt tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat
     6301 attcttgctc aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat
     6361 accggcgcat ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa
     6421 gcatttatca gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa
     6481 ggagaagcac agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat
     6541 aaaaaattca atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc
     6601 attacggatc gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg
     6661 caaattgctg attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat
     6721 ttgatttcaa cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata
     6781 tgatacatga tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg
     6841 gtcggacgaa tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga
     6901 tcgccgtatt gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa
     6961 acgagcttca gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga
     7021 ttccattaac gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt
     7081 tcgatttagc ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg
     7141 ggaaaccgca accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg
     7201 attgtcttgt tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg
     7261 gaacaattat tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc
     7321 taaataaaga tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca
     7381 catccgttaa ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac
     7441 ctccttttat aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt
     7501 gaaatatagc agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc
     7561 tatctataat taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa
     7621 tcatcaggag tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt
     7681 tccactagtt cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac
     7741 aaagaggaaa taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct
     7801 actaggtaag tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct
     7861 tttatcatgc gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata
     7921 tagtcactaa tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat
     7981 tcatttaatg actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc
     8041 attatcctat cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca
     8101 acttcgtttt cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac
     8161 caacccgttc tctgagtgtt gattctataa tatttt
//
LOCUS       DASJOX010000110         8083 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJOX010000110 DASJOX010000000
VERSION     DASJOX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.111"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(123..971)
                     /locus_tag="U4A12_002273"
     CDS             complement(123..971)
                     /locus_tag="U4A12_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1774616.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1195..3135)
                     /gene="thrS"
                     /locus_tag="U4A12_002274"
     CDS             complement(1195..3135)
                     /gene="thrS"
                     /locus_tag="U4A12_002274"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN1774617.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3472..3651)
                     /locus_tag="U4A12_002275"
     CDS             complement(3472..3651)
                     /locus_tag="U4A12_002275"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN1774618.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3698..5521)
                     /gene="cadA"
                     /locus_tag="U4A12_002276"
     CDS             complement(3698..5521)
                     /gene="cadA"
                     /locus_tag="U4A12_002276"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1774619.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5799..8078
                     /locus_tag="U4A12_002277"
     CDS             5799..8078
                     /locus_tag="U4A12_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1774620.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2372 a         1710 c         1460 g         2541 t
ORIGIN      
        1 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
       61 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
      121 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      181 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      241 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      301 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      361 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      421 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      481 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      541 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      601 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      661 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      721 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      781 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      841 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      901 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      961 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     1021 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1081 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1141 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1201 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1261 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1321 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1381 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1441 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1501 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1561 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1621 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1681 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1741 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1801 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1861 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1921 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1981 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     2041 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2101 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2161 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2221 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2281 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2341 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2401 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2461 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2521 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2581 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2641 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2701 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2761 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2821 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2881 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2941 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     3001 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3061 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3121 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3181 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3241 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3301 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3361 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3421 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3481 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3541 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3601 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3661 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3721 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3781 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3841 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3901 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3961 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     4021 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4081 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4141 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4201 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4261 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4321 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4381 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4441 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4501 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4561 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4621 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4681 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4741 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4801 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4861 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4921 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4981 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     5041 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5101 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5161 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5221 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5281 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5341 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5401 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5461 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5521 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5581 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5641 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5701 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5761 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5821 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5881 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5941 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     6001 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6061 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6121 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6181 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6241 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6301 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6361 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6421 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6481 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6541 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6601 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6661 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6721 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6781 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6841 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6901 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6961 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     7021 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7081 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7141 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7201 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7261 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7321 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7381 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7441 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7501 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7561 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7621 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7681 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7741 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7801 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7861 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7921 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7981 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     8041 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaa
//
LOCUS       DASJOX010000111         7923 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJOX010000111 DASJOX010000000
VERSION     DASJOX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.112"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..995)
                     /locus_tag="U4A12_002278"
     CDS             complement(21..995)
                     /locus_tag="U4A12_002278"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1774621.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="U4A12_002279"
     CDS             complement(1075..1950)
                     /locus_tag="U4A12_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN1774622.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="U4A12_002280"
     CDS             2080..3987
                     /locus_tag="U4A12_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN1774623.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="U4A12_002281"
     CDS             complement(4528..7806)
                     /locus_tag="U4A12_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN1774624.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2250 a         1665 c         1466 g         2542 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ata
//
LOCUS       DASJOX010000112         7649 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJOX010000112 DASJOX010000000
VERSION     DASJOX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.113"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..179)
                     /locus_tag="U4A12_002282"
     CDS             complement(<1..179)
                     /locus_tag="U4A12_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1774625.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="U4A12_002283"
     CDS             complement(192..1295)
                     /locus_tag="U4A12_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1774626.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="U4A12_002284"
     CDS             complement(1385..2341)
                     /locus_tag="U4A12_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN1774627.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="U4A12_002285"
     CDS             2622..4556
                     /locus_tag="U4A12_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1774628.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="U4A12_002286"
     CDS             5013..5429
                     /locus_tag="U4A12_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1774629.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="U4A12_002287"
     CDS             5443..6009
                     /locus_tag="U4A12_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN1774630.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="U4A12_002288"
     CDS             6032..7018
                     /locus_tag="U4A12_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1774631.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="U4A12_002289"
     CDS             complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="U4A12_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1774632.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2192 a         1466 c         1578 g         2413 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DASJOX010000113         7644 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJOX010000113 DASJOX010000000
VERSION     DASJOX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.114"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..2348
                     /locus_tag="U4A12_002290"
     CDS             21..2348
                     /locus_tag="U4A12_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1774633.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2474..2803
                     /locus_tag="U4A12_002291"
     CDS             2474..2803
                     /locus_tag="U4A12_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1774634.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2843..3847
                     /locus_tag="U4A12_002292"
     CDS             2843..3847
                     /locus_tag="U4A12_002292"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1774635.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3844..4290
                     /locus_tag="U4A12_002293"
     CDS             3844..4290
                     /locus_tag="U4A12_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1774636.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4440..5255
                     /locus_tag="U4A12_002294"
     CDS             4440..5255
                     /locus_tag="U4A12_002294"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1774637.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5461..6633
                     /locus_tag="U4A12_002295"
     CDS             5461..6633
                     /locus_tag="U4A12_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1774638.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6795..7289)
                     /locus_tag="U4A12_002296"
     CDS             complement(6795..7289)
                     /locus_tag="U4A12_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774639.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2572 a         1450 c         1524 g         2098 t
ORIGIN      
        1 attttttatt gagaggaggg atgaaaatga ttaagaaaac tcaaaataaa atccaacgta
       61 tatgtcaaaa acattttgtt tctagactca aagctgtatc taaaagaaaa tacttacatc
      121 ccattatgat ttcgatcatc ttgattttta ctttcttagg gagcttatca agtatttatg
      181 taaacgttta tacagaaacg tcaaattttg ctaaccaaac gacagaggag acaaatagtc
      241 caccctattc tgaaatacag tccatagaaa gcaccaatca gtctagcaac aacgagacaa
      301 cctcaaccac atcctcgact caactgattg atccgacaga aaaagaaatc acagcagcaa
      361 aagcagcagg ggagaaagct tattcagaaa caggtagaag ccagacattt agtactgttg
      421 cggccactgc gaatttcaac ggacatacca taccagatgg atatacagtg atatatgcca
      481 aagatggagt cattgttgcg gttaacggat cgatgagtgc ggataaaact gcgttacaag
      541 gcgtaaccaa agcaatcgct tttattgata accgtccaga tatccataat ctgtttattg
      601 gctccacagt aaatggtact actatcacat tcccaaaaag tatctatatg ggggtggcta
      661 ccgctggaaa tacgatcgat tcagttacac ccaatgcgga taaaagttat tcttttacac
      721 cctcaacatg gggagggctt cctgccggag gaggtatgag tactttttca atcggtatac
      781 agttagcaaa tgatattcca ttcacggggt actcgatcaa aaatgggcag atcgttgatg
      841 gtaagggaaa tgtttcaaat tttccaacct atcaagcagc aacggttcag gtcaatgcga
      901 atcggacagg aactttttct tatacagtca atggtggagc agcaaaaatc ggaacgtacg
      961 ggactacatt atctggaatc catgtgggtg acaaagtggt tatcactcca acagcagtaa
     1021 cgggctattc ttttgcacaa acaaacggtt ctttcacagc cctagaaagt aacgtcgata
     1081 cggtgaccta tacaggcaaa caaacaccaa tcactattaa gtatcaagac tattttggac
     1141 agatgattgc tccttctaaa ataatgaatc ttacttacgg aagtgctgca caagacctga
     1201 cgactaatac gcctactatc aacggatata cctttacagc tgtttcagcg atagaaacaa
     1261 agaaccaatc agcaatgagt gttttagcta gtttagatac aaatggaaat gtaatcgtaa
     1321 aggacgcaaa tggaaaacag atttctgaag tcatcctata ttataagaca aatgcaacag
     1381 tatcaatcaa tgcaaatggc agtaagtact atgatggtct ttctgttgta ccgatcgtta
     1441 aatatgcctt taatgataaa acagaatcaa catccctttt aaacaatgat ctcagtattg
     1501 atcaaatcac atggaatcca actgatttca aagcgatgaa tgaaaaggga gttgaagtaa
     1561 ctgcgcctac tgaaattgga acctatacgt cttggcaatt gacaaaaagc ggactagcta
     1621 aactagctgc cagaacaaac tatttattta ctatcgtgca gacatctgat gtctatacga
     1681 ttaagcaaat cagcggtgcg gtagagctca gtggcagtaa gacatatgat ggaaaagcag
     1741 gtgtcccgtc aatccatgtc aagctagcag agggtgtcac tagttcgctt actccagtac
     1801 aagtggcact tagcagtatg gattacacag cagatgcaca atcagcaaaa aatatggtta
     1861 atgctggcag ctatacaatt aaactaacga atagcggaat agacaaagtc aaagcagcaa
     1921 atcccaagtt gtcatttact gatatagcca acacatcagg aacttatacc atctataaag
     1981 ctaacgcagt gattactgtc gatgacgctg cctttaatta tgatgcccaa agtcactcga
     2041 tttcggttgg aaatctccac gttaccggtg ttgtcttagg cgaatcattg gattataccc
     2101 taacagttaa cagccggaca gatattggat cacaaatagt aacgattagt ttaggcgaag
     2161 gaagtgtaaa caataattat acgattatcg caacagatac cgctaagttg acgatcaatc
     2221 cgactctctt gcttccttcg acaggtggga taggtctact gccctttgtc tttttaggac
     2281 tgatctttat tttttctggc ttcttttatt ttatccatcg taagaaaaaa gcaggtgaac
     2341 agcgatgaaa aaaacaaaaa atgtacgtgt ggatatattt gctgttctct tttttatcct
     2401 actggtcttt tttataggtt ctccgactgt gtcagctaga gaatcaccta ttacatttac
     2461 tttgcataat cgaatgttcg aaagcgataa gatgccaagt atcagtcaaa acgacggaat
     2521 aaatatcccg gataactttc aaggagtaaa agggttgaac ggcgttactt tcgctatata
     2581 tgatgtctcg gatgaatttt acaagctgcg gtcagaagga agtagtgtgg aagacgcaca
     2641 aagaaaacta gcacaaaaaa gtgatagtga aaaaatactg gcagaaaatg tgacaaaaac
     2701 agtagacgag gaagagggca ttgcttcttt tagtgcatcg gacaaagatg agcaaggtcg
     2761 tgacgctgtt taccgattta cggaaataaa gacttcagat taagtaaaag aaaaatcagc
     2821 accatttgtc gttgtgcttc ctgtgacaga ttcatctaat cataaattga cgaccatcca
     2881 cttgtatcca aagagcgaac aaaaaatacc tgctgcactc acattaacaa aaacagtaga
     2941 gaataagcag acagattttg cagatgggga taaagtccct tatctcatca ccgtaacaat
     3001 tcctgagaac atcaacgaga taggaaccta tacgatcaaa gatacagctg atcctcagtt
     3061 atggctagaa ccagaaacga tcgatttttt gattggtgga gacaagattc atacatttca
     3121 cacacaaaaa ataaaacatg gttttgtctt atcagattct ggaaaagatc ttagttcgtt
     3181 tgctggaaaa aaattaacga tccgttacca aatgacattg aaagaaaaca gtgaaaaaac
     3241 gacatttgat aacgatgtac gtttgacgac tgacaatcaa acagtagaaa cacatcttgc
     3301 catccaaaca ggaggcaaac aatttgtcaa agtggatcgg caaacgaaac aaaaactagc
     3361 agatgctcaa tttctagtca aaaaaaatga ccaatactta gcgcaagaaa acgggataaa
     3421 tcattgggta gccaaagatg attcaagagc aacgattttt acttccagta aagatggaac
     3481 attttctgtc catggattgt cttttggaag ttacgaacta gtggagatta aaccgcctaa
     3541 aggatatata ttgagtcaat caaccattct ctttgaagtt gaaaaaggca gttatgatcc
     3601 gcttcatcct ataccattgg aaatcatcaa cgaaccaaaa gcaaatccga cgaataatgg
     3661 aaaaacgcca gttcagatgc agacaaatgg tccatcaaat gaaaaaaatg gaggaagctt
     3721 tccaaaaaca agcgaagaga tgcttggagg tttctcaatt ttagggttat tgctagtcct
     3781 ttcaacaggg acagcttggt tttataaaaa acaacaaaaa ggaaacaata ggagggaaat
     3841 aaaatgaaaa acatcaaaac aaaaatatgg acgtttttag gaactgctat catgctgctt
     3901 cctttcgttc tcggcctagg aactgcagag gtttctgcag ccgtatcacc tacaccagaa
     3961 aatgttacgg ttaatttgca taaattgaaa ttcacttcag caccagaaaa tcaaataaac
     4021 aacggaacgg aattgacatt tccgaatagc gaaccgttga acggcgttga attcaacgta
     4081 tatgacatca ctgctacgta ctatccaagt aaagacacag cagttccagc agacgctaca
     4141 cccttcgcaa gtgtaacgac ttctggtgaa ggattagcta acctgactct tccaggtaaa
     4201 tctgatggga aagatgctgt atacgtcttt gtagaaacac caaagcccgg tgtcgaaacg
     4261 tcgccaaata tcgtgttaag cttaccctag accctgacaa acctttagac acgattcacc
     4321 tatatgctaa aaatgtcatc aaagaaagca atatcaaaat ccagaaagct gggaactttt
     4381 caggggtaga taagttagca ggggcgcaat tccagatcaa aaattcagag aatcaattcg
     4441 tgaccggttt tgatgaaaac actggtgtcg ctctctatga tcctgctgag tcagcgttga
     4501 cattcacaac tgatactgaa ggatccatca gtgtcaatgg attaatagac ggcaattata
     4561 ctttagttga aacaaaagca ccagatggtt atcaattaac caatccacaa aaagaaacta
     4621 cttttatagt aaaaaacgga aaattgcaac aaacaaccgg tatcatccat ggcgcagatg
     4681 aagccaatct tgggaatcca aacggtgtag tcaatcggga aaatacaact acggataaca
     4741 cgattactgt agaaaatgtc caaaactacg gtgatttcca cttcatcaaa caagatgtga
     4801 acacgcaaga aaaattagcc gatgcagaat tttctattct aagagcaaaa gatccaactg
     4861 ttaaattaat gaaaaaaata gcaaaccttg cctcaggaga atacccatat gcttggtcag
     4921 atgaagtcac aggttctgac tgggaagaag tgaaattgat ttctgattca gaagggaaat
     4981 tttcaatcga agggctgaaa caaggggcat actgccttca agaaacaaaa gcaccagaag
     5041 gctacgcatt acctaagaat gaagctgcgt acactgaatt tacagtagat gatgatccaa
     5101 caacttctga tgaaacaacg atcgataacc aacctaaagg aatcttgcct tcaactggtg
     5161 gtatggggat catcgtattc atcttggtag gtactgcttt agttggagga gcagtgatct
     5221 atttcaaaaa acgccatcaa gaaacagaag cttaaactat tggaaacaaa aatcatacga
     5281 taaataaacg atagtgattc aaatggggag aaaggttgtg acaagtggtc ggtctcttgg
     5341 tattcgctca acaaacggca aaataggagg aactattttg ccgtttgctt cgctaatact
     5401 caggtcaagg cgctgatttc acaacctctt catatacata ttagaaaata ggtggacatg
     5461 atgaataaaa gcagaataag aatactggac atcttcatgg caatcatttt agtcgttggg
     5521 atcgggatct tttcttatcc ttttgtagaa gattcactga atgattttct tgcccagcaa
     5581 atgatcatcc attatcaaaa gcaagcatcg aataagaatt cagaggaaat aaaaaaacag
     5641 caggaaaaaa tgacaaagaa aaaccaacag ttggcagagc aaaacgtatc accagggata
     5701 ggcagtttca accaagcagt tgatgcaaaa gcattaaaag atttacctag taatgccttt
     5761 tttatggaac atatgcttgg agtcatcgaa atcccaaaaa tcaatgtcag cttacctata
     5821 ttcgatcaga caacggaaat atttttgcaa aaaggaacgt cattattgga aggaagttct
     5881 tatccgactg gcgggaaaaa cacccatgcc gttctttctg ggcatcgtgg actcccagaa
     5941 gcaaaattat ttacagattt gcctaagttg aaaaaaggag accagttttt catccagatc
     6001 aatggaaaga cattggcgta tcaagtagaa aaaatccaag tggtcttacc tgacgaagta
     6061 gattctttgg gtatccaaaa aggcagagat ctcgtgactt tgcttacctg cactccttat
     6121 atggtcaata cacatcgact gcttgtgacg gggcatcgta ttccttatca agcaaaagag
     6181 gcaaaaaaag cgatccaagg aattgatcaa tggaaaaaat ggaaattttt tatttggttt
     6241 atcggtatac tacttggatc aatcggctta gtttggctac tcatcgctta tttagattct
     6301 ttggccatcg ctaagcgaaa ctatccttta tctttttatg taaagaacac aaacggacga
     6361 ccaatcgaag gaatggtctt ttctgtaaaa acgctcaacg gcaaacacta tattactaga
     6421 gaaaaagttc catttgtgaa agcttctgat gaatacggac tagtgagatt ttctgattta
     6481 aaaggcagga actatcgctt gcaacatgag gagctcctgc ttaaaatcca tgtaaaacat
     6541 aaacattcga aacagttttc aatgaaattg aaaaaaggaa gatataaact gcgaaaagaa
     6601 aaagaggtgt actatttgat tgagaaagaa tgaatgaaga tgtccaaaaa agcagaggga
     6661 acagactaaa tgaaaaaaga gagaagctga cgaaatggac gtgtaaactg ctatttttgg
     6721 tttactatct ggaacgccag cttctatttt tttatgtttc agaagaattt tattattata
     6781 gtataacttt tgaattagtt tccataacat ttggagcaag gggttaaacc acgagcttga
     6841 gcttcgctta aagtggcagg ggtatacgta ccatttccac atttatgtgt atggtatttt
     6901 gaaccggtcg gtgtgacata gacagtttgt tcattatttt gctcaggagc tgcggcttgt
     6961 gcctgctgag aagcctgttc agctgctgct tgctgtcgcg cctgttctgc ttgttgctgt
     7021 gcgattgctt cttgcctcgc ctgttctgct tgttgctgtg cgattgcttc ttgttgcgct
     7081 tgttctgctt gttgtcgtgc aatttcttct tgctttttcc gttcttcttc tgcttctttt
     7141 gctttgattg tggaatcaat tgcagcaaga cggcttgtca actcttgttt gttttcaggt
     7201 agcgcttgga tagctgctga agcagttgca tagttttctt ttgtaggatt tgcttcagct
     7261 tgtgacaaag cagattccgc ttctgtcact tttttttcta gtgcagcttt ttccgctttt
     7321 tgtttctctt cttctttttt cttttcaatt tgttctttcg atgaatctgc cgtactttcg
     7381 gagatcgtgg tactgctgct ggaagcctga gctgaacttg tgctttgagt agtagacgta
     7441 gtagcaccac acccactcaa caaacttcct gcgaccaaaa acaacgcaac taattttttc
     7501 actaaatttc ctccttaagt aaaaaacaac cgttttcaat aaatgtcctt ttcaattgtt
     7561 tacttacttg ttataataat tgtgttatga atagtgattg aggaaactta atctaatccc
     7621 gtgttgatac acgggatttt ttgc
//
LOCUS       DASJOX010000114         7625 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJOX010000114 DASJOX010000000
VERSION     DASJOX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.115"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(108..632)
                     /locus_tag="U4A12_002297"
     CDS             complement(108..632)
                     /locus_tag="U4A12_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774640.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(625..1098)
                     /gene="tsaE"
                     /locus_tag="U4A12_002298"
     CDS             complement(625..1098)
                     /gene="tsaE"
                     /locus_tag="U4A12_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN1774641.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1221..2201)
                     /gene="pta"
                     /locus_tag="U4A12_002299"
     CDS             complement(1221..2201)
                     /gene="pta"
                     /locus_tag="U4A12_002299"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN1774642.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2354..3034)
                     /locus_tag="U4A12_002300"
     CDS             complement(2354..3034)
                     /locus_tag="U4A12_002300"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1774643.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3160..4029
                     /locus_tag="U4A12_002301"
     CDS             3160..4029
                     /locus_tag="U4A12_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1774644.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4055..4570
                     /locus_tag="U4A12_002302"
     CDS             4055..4570
                     /locus_tag="U4A12_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774645.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4617..5210)
                     /locus_tag="U4A12_002303"
     CDS             complement(4617..5210)
                     /locus_tag="U4A12_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774646.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5235..5414)
                     /locus_tag="U4A12_002304"
     CDS             complement(5235..5414)
                     /locus_tag="U4A12_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774647.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5491..6000)
                     /locus_tag="U4A12_002305"
     CDS             complement(5491..6000)
                     /locus_tag="U4A12_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774648.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6096..6476)
                     /locus_tag="U4A12_002306"
     CDS             complement(6096..6476)
                     /locus_tag="U4A12_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1774649.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6806..7555
                     /locus_tag="U4A12_002307"
     CDS             6806..7555
                     /locus_tag="U4A12_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN1774650.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1580 c         1355 g         2522 t
ORIGIN      
        1 gtttattcaa aaaaaatgga ctgcgacacg acttttgtca cagtcccttc gtgtgttttt
       61 ctgccttctc aaaaggatga cagtttctca ctcttttcct atttcttcta ttcaatcaaa
      121 taactcattt tatatacatc taaaaattca cctagatctg ttcttgcccc tctaggcatg
      181 accgcttcga tttggaagcc catttttcgg taaaggtgta cggctctttc attacgaact
      241 tgaacgtcaa gctccaatcg aaaaagtaca cccatttcct tcgcccaact gatgatttct
      301 tctagcataa gagtgcctag tcccattccc caatattcct gcagaatact gataccgact
      361 tctcccacgt gagacaaacg aaattgactg tctgccttga cagatgctgt tccaataatc
      421 gtcccttcat agattgcaag cagcaataaa ttattattgc tttcgtatat ataatccaac
      481 tcttcttcta aggtttctgg tggtaacagg agttctgctt catccaaaac taaaaattct
      541 gtctccttat taacttgtcc catcatcaaa agaatgtctt ttgcatctcc tggcactgct
      601 tcccgcaaga tgatcccaac ttcttcactc atgattttca gcccattttt ctagaattcg
      661 ctgtttgaag gtttcacttt gctccccgta tgagattagt ttcacatacc gcttttctaa
      721 atctgtatca cttttttcta aaatcagttc caaataatcc tccggcagtg cttcttctaa
      781 tagatttccc cattcaacaa cggaaagccc atcaccttca aaatattcat ctaaccctaa
      841 atctgctccg ctagctgcta cccgataaat atccatatga tacaacggaa tccgaccttg
      901 actatattct ctaataatcg tgtatgtcgg acttttgatc atttgcgtta tccctaatcc
      961 ttgggcaatt cctttcgtca acgttgtttt acccgcacct agatcaccag tcaatatcag
     1021 attgtcacct ggcattgcgg aatgaccaat tgcctctgcg aattgttctg tcattgttaa
     1081 tcctgatagt tcgatcattt ttcatcttct cccgctcttc tttctttttg ccagtatatc
     1141 gcgtaatgaa gtttttttaa agaaaaaatg aactagtcaa gaacagcagc tgctgttttt
     1201 ggctagttcg cgtgtatttg ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat
     1261 ttatatacat cttcttcatt tgctccacga gacaaatcag aaaccggttt gttcaagcct
     1321 tgtagaattg gtccgattgc ttcaaagtta ccgaaacgct gagcaatttt atagccgata
     1381 tttcctgact gcaattctgg aaatacaaag actgttgctt gaccagctac ttctgaatct
     1441 ggcgctttca gttgtgcaac tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg
     1501 atcatgtatt gtggcgctag ttttttagcg attttagttg cttcaactac tttgtctact
     1561 tctggcgatt tcgctgagcc ttttgtcgag aagctcatca ttgctacttt tggttcgata
     1621 tcgaacaatt ctgctgtttt tgcagaatct acggcgattt cagccaattc ttgcgcagtt
     1681 ggattcacgt tgatcgcaca atccgaaaag acatattttt cttgatctct tccacgtacc
     1741 atgatcatcg ctccgcttgt ccgactgatg ccaggctttg ttttgatgat ctgaagtgct
     1801 gggcgaacag tgtcgccagt cgaatggatc gctccagaga ccattccgtc tgcaatcccc
     1861 atatacgtaa gcatcgtacc aaagtaattc acatctttta aaatcttttc tgcttgttct
     1921 ttggatgcct ttccatttct acgttcaaca aaagtatcca ccatttcatc ccacttatcg
     1981 taattatcag ggctgatgat cgtaaagcta gatgttttga ttcctctggc atgcgctgca
     2041 tttcttattt cttcttcgtt ccctactaaa atgggttcca caagttcttc tgctttcaac
     2101 cgggcagctg cgcctaaaat acgagcatca gtagcttccg ggaaaacgat tttgatccca
     2161 cggcgaacaa ttttaaatct taaactatca aacaattcca caagatgacc tccagacgtt
     2221 tttatatgta ttatcataca ctattccaga aaaaaagaac agtttatttt atctgtatca
     2281 gtcaaaaatg ctggaaaaat taagtttttc acatacaaag cgaactgcac tataacagga
     2341 aagcgctgtt ttattacact gtttcaggca gttgccaatc aatcggttct tctcctaacg
     2401 caaggagcgc atcattcgtt ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag
     2461 ataacggact gggatgtgga gatgttatga tgatatggcg actcttatcg atcattttga
     2521 ttttttcttg tgccggtttt ccccacaaga taaacacgat tggtttttcc cgttcattta
     2581 atttttcaat gatcgtgtct gttaatcttt cccaaccttt cccgcgatga gaatacgctt
     2641 gcccttcccg aacggtaaga acggtgttga gtaaaaggac tccctgttta gcccatgaca
     2701 ctagatttcc atgttgaaca ggtgcgattc caagatcatc ataaagctct ttatagatat
     2761 ttcttaacga tggcgggatc tttacgccag gttgcacaga aaaagataag ccatgagctt
     2821 gattttctcc atgataagga tcttgtccta aaatcaccac tttaacttct tcataaggag
     2881 tcaattccaa tgcttcatag atatgataca tatctggatg gatcttttgg gacgcatatt
     2941 ctttttttaa aaattctctt agatgcagat aataatcttt tgaaaattct gaggacaaaa
     3001 cttcttgcca actgttatga atgatttctt tcaaaaactc cacctcctaa taaaaagaat
     3061 aactcacatt ttcttcaatc acaagggaca atcttcaccc tcagcacttg attaatatgg
     3121 taaactagga acaagaagca gaaaggaacg ataatcatta tgatcaaatt aattgcatca
     3181 gatatggacg gcacattatt agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg
     3241 atacgttttg caaatgatgc cggtgttgag ttcatggtcg ctactggtcg aaactatcaa
     3301 gaagctcgcg cagctttaga tgaggtagga atcgattgtg cgatgatcac tttgaacggg
     3361 gcacaggtat ttgataaaga aggaaattca ttgtttaccg tgtcgattcc aaataatcaa
     3421 gcaatctctg ttttagatat tctagatgct aacggtattt actatgaagt agcaaccaac
     3481 gatggtcttt attctgaaaa tcaagcgaaa cgaattgaaa gttttgcttc catggtggct
     3541 acccatttac ctcaccttac gtataaaatg gcgattgcca tggcctcagc taagctggag
     3601 ttattgcaca tcacatacgt ggacagtatt cgtgctattt tggaagaaaa gaatctggaa
     3661 gttttaaaaa ttatttgttt tcatacagaa gggccagctg tattaggtcc agttggaaag
     3721 cagatttctc ttttaggcga tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg
     3781 aaccataaaa acgctcaaaa aggaattgct gttgcccatg tcgctcacga gcgtgggatt
     3841 tctttagaag aagtcatgac gattggcgat aacttcaatg atgtcagcat gctgcaagta
     3901 gcaggagtca gtttcgcaat gggaaatgca gaaatcgaag taaaagatta tgccaaatat
     3961 acaacggaca caaaccttga atctggcgta ggaaaagcca tattgcgcgc gattgcagaa
     4021 gacttgtagt tttatctaga aaagagggat atagatgtca gaatttttca ttcaggaaaa
     4081 gcagcttgga cgtgcaactc ggacaattgt caaaaacgaa aaaggacatt ccctgttttt
     4141 gatggttggt cgctggggaa caaagggcga tgcattgtct ctttatgcca tgaatggcga
     4201 tctcgttgct cacatcaagc agataagcct gacattcggt acgcgttttg agctgtataa
     4261 agagttcgaa aaggtgggaa ccatgcagaa aatattcaat tggcccggtg acttttatta
     4321 tattcgtaga cttcactgga cagtccaagg tgatatctat aatcatcaat atcaaatcca
     4381 tcatttcaat cagcaaatca tgaaaatgga taaagcaaca cttttttcag gagattacta
     4441 tgtattggat gtctcaaatg atgaagatgc tccagtttgt atttgtattg ctgcggtttt
     4501 agattactgg ctctaccgta aagacaaaag tcgcgataag cctgtttctt accgacttaa
     4561 cttaagttaa aaagatgtac ctgaaatgcg gaaatacgca tttcaggtac atctttttag
     4621 atattctttt gcatatgatc ataaagatga ccactgatca tccgctgacc gacaactttg
     4681 aagtcatggc gttcgtataa ttttttagcc tttggatttt tttcatccac acttagaccg
     4741 atgacggagc gattagcttt cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg
     4801 atgccttgtc ctctaaaatc ttctgataca ctgatagagt ccaaatacca ttcgttggga
     4861 aacgtttctt tatcaatgaa caaacgtgta gacgcatcta gctgatattt ctctaagata
     4921 ggtaccagtg gctgatcgat gattgattct tcttcatctg tataaccgta agcaactcct
     4981 gcaactttac cgtctatttc ttctacgata cctcgtgaat aactgtaacg ataatttgga
     5041 tcagtgattg cttcttctaa aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat
     5101 ggcagttcca tatcttttag aatcactaag atcaatgggg cgatttctgg agcatcttct
     5161 tttctcgcaa aacgaatcat agtttcctct ccttttctcc attctaacat gcagaaaaag
     5221 aaatcgcaaa atcgttattt taaaaaatct tcaatctttt cagtaatgaa ataaccatga
     5281 gtaacgattc ttgccgcttc agttcggcaa taagggcaga cgactggcat ttctttatca
     5341 ttatgttcga ctacattatt gacttcttcg tatgcaaatt tttttccgca attagggcat
     5401 ttcatttttt ccatgttatc tcaacttcct ttcatcttta ctctcttagc ttactcttat
     5461 attgtaaaaa agagaagcaa tatccttctt ctatgcagat atccatacct gacggtcctg
     5521 ttctccatct attaatcgga ctttataata aggacgttca tctttgacta gaagtgtcca
     5581 ctctttcact tttgcttttt tcccgtagta gctgactgct tccttcatcg ctgcttgcgg
     5641 ttgtttgaac tcttccgttt tttctatatc aaaaagatgt tctgcggcct tttccgtgtc
     5701 atttcgtacg gttgttttcc cagtggatgg attgactgct atgacttgtc cttcacaaaa
     5761 aaactggtat tcatatgttg cattcgtagg attctccaaa tctttttcaa ctgcaaaatg
     5821 aatagcagat atgttatgtt cttttgtttc gttttttacg attgctgctg cttttttcaa
     5881 tgagatccga tattcctggt cactaatatc atccatgtga aaagcggcat tgagcagtcc
     5941 cgtcataccg gtaatcccta ttacgatgag ggcagcaaca ccaaacatca tttttttcat
     6001 tcttcattct cctgtttctt ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg
     6061 aatgaaacga aaaaaaagtg acaattttgt tatctttatt cgtgcagttc taatggcaat
     6121 ccgtctggat caaagaaaaa cgtcattttt tttcctgtaa aggtatcttc tctaatcggt
     6181 tctgattcga tccctaagga ctgcaattct gcaattgttt cttcaatatc gtctactttg
     6241 aaagcaagat gacgcaagcc acaagcttct ggatagtttg gtcgttgtgg cgcattagca
     6301 attccaaaaa tctccaattc agcacttcct agtttcagat ctaatttata gtcatctcgt
     6361 tctttgcggt aattttctct gattacttca aatcctaatt tattaacata aaaatcacgt
     6421 gatttctgat aatctgaaac aatgatggct atatgatgga tcgttgtaag gttcatatga
     6481 gcactcctat tcttcgttta agctcggatc tatattaatc tccagttcca ttgtaaatgt
     6541 gtcagattga aaaaaatagc tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga
     6601 aagtaggtcc tccaagacac tcttcctgtt cccaatataa taaatgaaag taagaaatta
     6661 tattttcaag aaaacaaaca gaaaattatc ataacacaaa ttatttataa ttgaaagact
     6721 agtttttttt caagaaaagc atacaataga ttgtgttagt aaatacagaa cacactacat
     6781 ttagtgtctt aaggagtcct catacatgaa gtcatatttt cagtttttag ctcatcagct
     6841 aaaaggctgg ccgcaacaaa attattatct tttctttttc agtcttggct gccaggtcat
     6901 gactcttgtc aatagtccca tcacttggct gtccgttatt acatttatcg gtaccacgct
     6961 tggtgtattg tgtattttat ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat
     7021 tttgtctgcg atttgtttta ttatcgttgg gttttcagca aagaactatt taagtatcgg
     7081 tgaacagatt gcttatatga tcacattgga tatccccgta ttgttaagta aagaatggaa
     7141 tcataatatg gcttcaaaaa tccgaaaatt cactggaaaa agctggttta tcgcaatcat
     7201 cagtactttc cttgtctatc ttgtttcagg attcctgatt ggtcgttata cagatgatcc
     7261 tcggccgtgg gtagatgcca ttgctttttc tattagcttg actgctggta tcatcagttt
     7321 cttgcgttac aacaaccaat attactggtg gttagcttca gggcttgctc aaatggtcct
     7381 ttggtatatt tcatttacgc atggatctgc aagtttggcg atgttcgtga acagctctgt
     7441 ttacttgctg aatgatgtcc ttgcatttac cgtttcacct tggtataacc aaaaagaacg
     7501 tgaacgtatg caaaaaatgg aagaagaata ctacaacgca caacaacacg cataaaaaaa
     7561 ctgagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
     7621 cggaa
//
LOCUS       DASJOX010000115         7613 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJOX010000115 DASJOX010000000
VERSION     DASJOX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.116"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..706)
                     /locus_tag="U4A12_002308"
     CDS             complement(14..706)
                     /locus_tag="U4A12_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1774651.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="U4A12_002309"
     CDS             complement(1143..1712)
                     /locus_tag="U4A12_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1774652.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="U4A12_002310"
     CDS             complement(1786..2109)
                     /locus_tag="U4A12_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774653.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="U4A12_002311"
     CDS             complement(2263..3075)
                     /locus_tag="U4A12_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774654.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="U4A12_002312"
     CDS             3429..4277
                     /locus_tag="U4A12_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN1774655.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="U4A12_002313"
     CDS             4422..5363
                     /locus_tag="U4A12_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1774656.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="U4A12_002314"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="U4A12_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2269 a         1505 c         1371 g         2468 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt ttt
//
LOCUS       DASJOX010000116         7560 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJOX010000116 DASJOX010000000
VERSION     DASJOX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.117"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..787)
                     /locus_tag="U4A12_002315"
     CDS             complement(59..787)
                     /locus_tag="U4A12_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1774657.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="U4A12_002316"
     CDS             complement(800..2080)
                     /locus_tag="U4A12_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1774658.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="U4A12_002317"
     CDS             complement(2221..2823)
                     /locus_tag="U4A12_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1774659.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="U4A12_002318"
     CDS             3068..3274
                     /locus_tag="U4A12_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774660.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="U4A12_002319"
     CDS             3586..4305
                     /locus_tag="U4A12_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1774661.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="U4A12_002320"
     CDS             complement(4386..5069)
                     /locus_tag="U4A12_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN1774662.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="U4A12_002321"
     CDS             5287..7473
                     /locus_tag="U4A12_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1774663.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2710 a         1276 c         1421 g         2153 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
//
LOCUS       DASJOX010000117         7420 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJOX010000117 DASJOX010000000
VERSION     DASJOX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.118"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(193..1641)
                     /gene="cls"
                     /locus_tag="U4A12_002322"
     CDS             complement(193..1641)
                     /gene="cls"
                     /locus_tag="U4A12_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1774664.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1793..2686)
                     /locus_tag="U4A12_002323"
     CDS             complement(1793..2686)
                     /locus_tag="U4A12_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774665.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2670..3356)
                     /gene="ftsE"
                     /locus_tag="U4A12_002324"
     CDS             complement(2670..3356)
                     /gene="ftsE"
                     /locus_tag="U4A12_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN1774666.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3461..4562)
                     /gene="prfB"
                     /locus_tag="U4A12_002325"
     CDS             complement(join(3461..4489,4491..4562))
                     /gene="prfB"
                     /locus_tag="U4A12_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN1774667.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4688..7222)
                     /gene="secA"
                     /locus_tag="U4A12_002326"
     CDS             complement(4688..7222)
                     /gene="secA"
                     /locus_tag="U4A12_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN1774668.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1990 a         1645 c         1292 g         2493 t
ORIGIN      
        1 tttgaaatgt gtctctttta atagcatata ttagcttatt tcttgctttc cccaaaaaac
       61 aagagatacc ccaaagttaa tttcgctcat gcgagacgct ttctccattt ctgacgtact
      121 ctcagaaatg gccccgttag aaaaggacac gagatctcca tctgttgatg gttctcatgt
      181 cctttttatg ttttaaagga gcggcgaaat caatcttgct aatccttctt tcatcttgat
      241 gattattcct cggttttcat acatttcttt tgtgagatgc gtagaaattt tcgagtcttc
      301 aaaaaaagct tttcttacac gtgaagccat tcgttcatca taaaccagtg tattgacctc
      361 aaagtccaag cgaaatgaac gaacatcgat atttgctgag ccaactgaaa caatgccacc
      421 atcgataatc atcgtctttg catgaataaa tccattctca tatgtttcga tccttgctcc
      481 atactcgatt agctctgcag cgaaagaata cgttgcccag taaacgagaa gatgatctgg
      541 tttgttaggt atttggatat gaacttttac gcctgaaaga agagccaact tcagtgcttc
      601 atgaatcgat ccatccggta tgtaataagg cgtttggatc aatatctccc gtttggctaa
      661 cgaaatcatt ttcaaataag tcattttgat ttgttcatgt tcggtatctg gtccgcttgt
      721 cacgatttga acagctatat caccagtaga gtgaatggac ggaaaaaatt tgggagaata
      781 gcctacttct tttgtatgct gagaattcca gtccatcaaa aaacggtttt gcaaggtata
      841 caccgcttct ccgtaaatac gcaaatgatt gtctcgccaa taaccaaatt tctttaccaa
      901 tcctagatat tcatttccaa cattgaaccc acctgtatat ccgattttcc catcaataac
      961 tacgatttta cgatgattgc gataattgat ccgaggatta atatacggaa caaatagagg
     1021 aaagaaaaat gcgacttctc cgcctgcttt tttcaaattc tgaaaaaagc tggaagaaac
     1081 ttgtgtggaa ccccatgcat caagaaggac tctgactttt acccctcgtc ttgccgcctc
     1141 tgtcaatgca tctcgaactt cttcacctaa tgcatcactg cgataaatat aatactggaa
     1201 gtggatatgg tcttctgcct gcttgatatc ttcaatcagc gcatcgaatt tttcacgacc
     1261 atctgtaaat aaagtgattt catttttagt cgtatagagc gtactttcaa ataccgtgag
     1321 catatagatc aattgtttca catctacttg tccagtaggc ggatgaggat aaagccctcg
     1381 catcaatgcc tgtttgtctt cttcaatttc caggttctta ccgattttcc cttggatctt
     1441 caaatcaaag attttctctt tggaaatccc tcttcctaaa aaaatatata aaataaatcc
     1501 tacaactgga atgaacatca aaacgagcac ccatgcccat gtttgagcag tttcctttct
     1561 ttctcgaaaa acgatgataa aggaaagcaa aatattcata atcaaaagga tcgtaaaaaa
     1621 attgtcaacg ataattttca cggcttcacc tcttcttcta ccaacttaag aatacccaag
     1681 ttgtttttta aagtaaaggg aaaaaagccg cttccttcta ttctcctagc caataaaaaa
     1741 agaaagctgg aacgaaactg tccaactttc tcattttatt tatattgtgg tattagaatt
     1801 tcaagaaacg tctcatagaa atgatcgagc caagtgaacc aatgatcaca ccaatgccga
     1861 ccattaaaag attgattttc caaacgatat tttgtggatg aatcaatgaa taatgggaac
     1921 gtaataatga tggattgatc aattggaaaa accatgcata accaaatgtg atgatcaaaa
     1981 tcggaagaat cgcaccgatc agaccgatcc agccaccttc aaggaaaaac ggccagcgaa
     2041 tatatccatt ttttgcaccg actaaacgca tgatttgaat ttcacgctgt ctagacaaaa
     2101 tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac gaacagcagc aatgccgctg
     2161 caactaatcc ccatgtccga actgcaccag cgatttggaa aatccgatct gaggaagctc
     2221 caccgtaatc agaacgagaa acattcttca aatctgctgc ttcttctgct acttgttttg
     2281 tataagaagg atcagtagca cttactacat acacatcata taacgggtta ttgtcacctt
     2341 caaacaattt ccaggaatca cccatttcgt tttggatttt tttcaattcc tgttctttgc
     2401 ttgaaaattc aactttttta acatgatcta agtcattcaa ttctttttct agctttttca
     2461 tctcttcgtc ttttgtaccg atatctacga atacagagac atcaacgttt ccttctatat
     2521 cttgagctaa tttagttgcg ttcatgataa ctgccatgaa tacaccgact aaagtcaatg
     2581 tgatcgttac cgcactgact gaagcaactg tcatccagcc gttacgtttc aaactcttga
     2641 tactttctaa taaatggcgg aaaaaggttc taatcatcgt atccatattc cccttccgct
     2701 tgatctcgaa taatgcggcc gttttcgata gcaattactc ggtgccgaat cgtatttacg
     2761 atcgtactgt tatgtgtagc catcacgaca gtagttcctt gggcattgat ccgatctaat
     2821 aatttcatga tttcccatga gttttctgga tccagatttc ctgtcggttc atccgcgata
     2881 agtactttag gcgtattcac tattgcccga gcaatagaga cacgttgctg ttccccaccg
     2941 gataattcgc taggaaagac tcgaactttg tgtttcaaac caacaaggtc cagcacttcc
     3001 attactcgtt ttttgatatc ccgaggtttt ctgccgataa cttgcatagc gtacgcaaca
     3061 ttttcatata ctgtttttct tggtaataat ttatagtctt ggaacaccac gccgatttct
     3121 ctgcgtagat aaggaacttc gcggtttttg attgctatta gatcatgacc tgcgacgttc
     3181 aaagttcctt ttgaagcttt ttcttctcga tacatcaatt tgatgaatgt agatttaccg
     3241 gctccagacg gaccaaccac atagacaaac tctccttgtt cgatgacaat tgagagattg
     3301 cggatagcgg tcgtaccatt tgagtacttc ttcattacat ccttcatttc aatcatggcg
     3361 ttctctccat atctttttca gtctttagct attatagcat ggcaatgttt caacatgctt
     3421 gcaaaatctt acaattttat ttcaatttaa caatgggttc ttattgtaga tgcattttta
     3481 aatagccgtc aataaataaa tcaaggtcac cgtccataac tgcttggaca tttcctgtct
     3541 ctacgtttgt ccgatggtct tttaccatag aatatggatg gaaaacataa gaacggatct
     3601 gtgaacccca gccgatttct ttttgttctc ctcgaagtgc agctgcttct tgttctttct
     3661 tttccacttc taattgataa agcttagctt ttaacatccc catcgcttgc tcacggtttt
     3721 tcaattgtga acgttgtgcc tgactagcta ctactgttcc tgtaggcaag tgagtgatcc
     3781 gtacagcaga ttctgtttta ttgatgtgct gaccacctgc accacttgct cggtacgtat
     3841 cgattttcaa atcatctgga ttgatttcta tatcgatcgt ttcatcaagc tctggcatca
     3901 catctaccga acaaaaagaa gtatgacgtc gtttcgctga gtcaaaaggc gagatacgta
     3961 caaggcgatg aacacctttt tctgatttca gataaccaaa tgcgttgtgt cctttgatca
     4021 gtagggtcac actcttgatt cctgcttcat cacctgcttg ataatctagt gtctctactt
     4081 gatacccatg ctgctctgcc cagcgtgtat acatacgtaa aagcatactg ccccaatctt
     4141 gggactccgt tccgccagcc cctggatgca attcaacgat ggcgttgttt cgatcataag
     4201 gaccatctaa caacatagat aattcatatg tcttcatttt ttcatctagc gcattgatac
     4261 gtgtttctgc ttcttcttgc atggctgcat caggttcttc ctgcaacatc tccaccatca
     4321 attcgatttc ttccagttct tcagcaagag aatggaattg atcataagtt tctttatttg
     4381 cattgttttc actgatcact ttttgagcag cttcgctatc atcccaaaaa ccaggttcag
     4441 ccatttttgc ttctgcttcc gcaatgtctt cttccagctg gtctaagtca aagagacctc
     4501 ctgaagcttg tgaccttttc ctgcatttct gtcaggagat ttcgaatttc ggatatttcc
     4561 atgttttttc cttctttcac tatacttttt tcattatatc tagtctaaca gaatcagcaa
     4621 ataacagaaa gaaggtgaga cgaattctta aaaaaagaat ttgtctcacc aatcctgctg
     4681 tcagaaatta gtttgcattt tttccatgac agttcttata ttttttcccg cttccacaag
     4741 gacaaggatc atttcttccg accttgttca catggattgg ctgttttttc gcgctagtat
     4801 tactattttc tttggcatct ccgtcttctg agtgagaagc tgttccttga gaaacctgtt
     4861 cacgctgtac gttttgacgg atctcagctt tcataaagat tcgagtgact tcatactcga
     4921 tggcgcctac catatcttca aacatcttat agccttctgt ttgatattcg actaatggat
     4981 tgttctgtcc ataagcacgt aagccgattg actgacgtaa ttgatccatt gcatcaatat
     5041 ggtctgtcca tttagagtcc acaacacgca agatcacgac tttttcaaat tccaacagtt
     5101 gctctggacc atttagttga gccgctttgg tattaaatac ttcttgtgct cgttcgttta
     5161 gatactcttt gatctcctga gctgtttttc cttcgatatc tgcaagagta atactatctt
     5221 catggacaag agtactgccg gcaaaatcga tgatactttc caaattccaa ttttctttct
     5281 ctaattgtgt atgcgcatct actacacgac tgattgtccg tttgaccatg ttcattagtg
     5341 gcttggataa gcttttttct tccatgatta cttcttgtcg ctgtttatag attacttcac
     5401 gttgttcacg catcacatca tcgtattgta agacgttttt acgtgtgtca tagttatttc
     5461 cttctacacg tttttgtgca gattctactt gtttcgtcag catcttgctt tggataacag
     5521 catcttcctc accgatcttc atccgatcta agaaagattt gattcgttct gaaccgaatc
     5581 gtttcatcaa atcatcttcc aatgacaagt agaactgtga cataccaggg tctccttgac
     5641 gtcctgcacg tcctcgaagc tggttatcga tacgacgaga ttcatgtcgt tctgtcccga
     5701 taactgctag accaccaagt tctcttaccc caagtcctaa cttgatatct gttccccgac
     5761 cagccatgtt ggtagcaatc gtcacagcac ctttttgacc agcattcatg atgatctctg
     5821 cttctttaaa gtgatttttc gcattcagca cttcatgagg aatacgttct tgattcagca
     5881 tatctgataa taactcagat gtttcaaccg caacagttcc gactaacaca ggttgacctt
     5941 tgcggtagcg atctttgata tcttgcacga cagcgtggaa cttgctttgt aatgttgggt
     6001 aaagcaaatc tggacgatca tcacggataa ccggacgatt tgtcggaatc tggtatactt
     6061 ggatgttata aatttctctg aattcttctt cttctgtttt agctgtacca gtcattcccg
     6121 caagtttctt gtacatacgg aaatagttct ggaaagtgat cgtcgccatg gttttcgttt
     6181 cgtcttcgat ctctacgcct tctttagctt cgattgcttg gtgaagtcca tctgaataac
     6241 ggcgtccatc catgatccga ccagtgaact ggtcaacaat cagtactttt ccgtcttgga
     6301 caacataatc gatatctaaa agcatgatat agttggctct caatgcttga tccaagtgat
     6361 gggtcaatgc agtattttca atgtcgtata agttatccaa accgaatgtt tcttctgctt
     6421 tttcgatccc tgcttcggtt aaactgattg tttttgattg tacatcgatt ttataatctt
     6481 cttcttcttt caaacgtttc acgaagttgt ctgctcttgt atataaagct gtggattttt
     6541 ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc aatcaagatt gagtcaactt
     6601 catcgacaat ggcataattc agcggacgtt gtaccatttg atggcggtaa acgaccatat
     6661 tgtctctcaa gtagtcaaat cctaattcat tattcgtact ataagtgata tcacaattat
     6721 aagcctcgcg tttttcatcc gaagattttg agttgatatt caaccccact gttaatccta
     6781 agaaattata aagttcgccc atttccgtcg agtcacgggt tgctaaatat tcattgacgg
     6841 taactacgtg taccccttcg ccagtcagag catttagata tactggcatc gttgcggtca
     6901 aggttttacc ttcaccagta cgcatttcag ggatatttcc atcgtgaagc acgattccac
     6961 ccatcaattg tacgtggtgt ggatataacc ccaatacacg ttttgctgct tctcggacga
     7021 ctgcaaaagc ttccggaagt aattgatcta atgtttctcc tttttggtat ctgcccttga
     7081 attcagcagt ttttgcctgt aattgttcat cagttaatgc tgccatttgg tcagcgtatg
     7141 cttcaacttt ttttgcaatg ctatctaaac gttttagttc tttcttatcg ttttcgataa
     7201 gtgaacgaat caaattggcc atgcgttgtg taatctcctt ttccttctgt gtattatcca
     7261 gactatgtag actttacttt tcattttatc attagttata gataattgaa atatcttaag
     7321 cgtattaaag taagaacttc ataatttatt catatttcat ctaaaagagg aggaaactca
     7381 aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
//
LOCUS       DASJOX010000118         7284 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJOX010000118 DASJOX010000000
VERSION     DASJOX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.119"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..2678
                     /gene="clpB"
                     /locus_tag="U4A12_002327"
     CDS             69..2678
                     /gene="clpB"
                     /locus_tag="U4A12_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN1774669.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2829..3515)
                     /locus_tag="U4A12_002328"
     CDS             complement(2829..3515)
                     /locus_tag="U4A12_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774670.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3493..3987)
                     /locus_tag="U4A12_002329"
     CDS             complement(3493..3987)
                     /locus_tag="U4A12_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1774671.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4007..5227)
                     /locus_tag="U4A12_002330"
     CDS             complement(4007..5227)
                     /locus_tag="U4A12_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1774672.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5374..7209
                     /gene="lepA"
                     /locus_tag="U4A12_002331"
     CDS             5374..7209
                     /gene="lepA"
                     /locus_tag="U4A12_002331"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN1774673.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2394 a         1393 c         1498 g         1999 t
ORIGIN      
        1 gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa ttaggagggt
       61 atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt gctgaagctc
      121 aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta tggaaaatct
      181 ttttacaatc taatcatttt ggtcgaaatt tctatacgga tgccggattg gatgtcgaat
      241 cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct ggtggaaacg
      301 tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa gcagaccaaa
      361 tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta gcgttgatga
      421 aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct gaaaaagaat
      481 tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa aaccaagaag
      541 aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc aaaagcggaa
      601 aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt attctttcac
      661 gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa acggcaatcg
      721 ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta aaagataaaa
      781 cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt ggtgaatttg
      841 aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt atcctcttgt
      901 tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt atggatgccg
      961 gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt gccacaactt
     1021 tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc tttcaaaagg
     1081 tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga ttaaaagaac
     1141 gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca gcagctactt
     1201 tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat ttgatcgatg
     1261 aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta gatcaagtta
     1321 ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa tcagatgatg
     1381 caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt gaagaagcaa
     1441 atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt tcagcaaaac
     1501 gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac tatgatttag
     1561 aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa ttgaaggaat
     1621 tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt accgaaaatg
     1681 agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt gtagaaggtg
     1741 aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt ggtcaagatg
     1801 aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg caagacccaa
     1861 atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa accgaattag
     1921 cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg atcgatatga
     1981 gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca ggctatgtag
     2041 gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat acaatcgtct
     2101 tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta caagtcctag
     2161 atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat acggttctga
     2221 tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct gatggaacga
     2281 ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc aaaccagaat
     2341 tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac aacgtgaaag
     2401 gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa gaaatcctgc
     2461 tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa ccagcatatg
     2521 gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta gctaaagaaa
     2581 tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt gatggacaac
     2641 ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag actataaaag
     2701 aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca
     2761 gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac
     2821 ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta acgctcatct
     2881 tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc
     2941 aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca
     3001 gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa
     3061 aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt
     3121 tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg
     3181 accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc
     3241 atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct
     3301 gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc
     3361 cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata
     3421 aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc
     3481 tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag
     3541 caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa
     3601 gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg
     3661 ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata
     3721 agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat
     3781 cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc
     3841 ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga
     3901 agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa
     3961 cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc
     4021 taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg
     4081 tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag
     4141 tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt
     4201 gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt
     4261 caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt
     4321 ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt
     4381 caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg
     4441 aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca
     4501 tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat
     4561 ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact
     4621 cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat
     4681 aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga
     4741 tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc
     4801 atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat
     4861 tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat
     4921 attctggata agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat
     4981 tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga
     5041 acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa
     5101 acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga
     5161 attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt
     5221 gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa
     5281 aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg
     5341 gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc
     5401 caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg
     5461 gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg
     5521 ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa
     5581 ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg
     5641 cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta
     5701 gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta
     5761 gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc
     5821 gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta
     5881 gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt
     5941 cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat
     6001 gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga
     6061 gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc
     6121 tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca
     6181 agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct
     6241 gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat
     6301 ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat
     6361 gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt
     6421 ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat
     6481 ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg
     6541 actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa
     6601 gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa
     6661 ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta
     6721 aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa
     6781 tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag
     6841 ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt
     6901 caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc
     6961 ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt
     7021 tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct
     7081 cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc
     7141 tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga ggacgaaccg
     7201 aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt
     7261 tgccaccggt ttgccaccgg tggt
//
LOCUS       DASJOX010000119         7120 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJOX010000119 DASJOX010000000
VERSION     DASJOX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.120"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            243..962
                     /gene="atpB"
                     /locus_tag="U4A12_002332"
     CDS             243..962
                     /gene="atpB"
                     /locus_tag="U4A12_002332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN1774674.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            1015..1230
                     /gene="atpE"
                     /locus_tag="U4A12_002333"
     CDS             1015..1230
                     /gene="atpE"
                     /locus_tag="U4A12_002333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN1774675.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            1321..1845
                     /gene="atpF"
                     /locus_tag="U4A12_002334"
     CDS             1321..1845
                     /gene="atpF"
                     /locus_tag="U4A12_002334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN1774676.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1832..2374
                     /locus_tag="U4A12_002335"
     CDS             1832..2374
                     /locus_tag="U4A12_002335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN1774677.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            2397..3953
                     /locus_tag="U4A12_002336"
     CDS             2397..3953
                     /locus_tag="U4A12_002336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN1774678.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            3967..4869
                     /locus_tag="U4A12_002337"
     CDS             3967..4869
                     /locus_tag="U4A12_002337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN1774679.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            4892..6298
                     /gene="atpD"
                     /locus_tag="U4A12_002338"
     CDS             4892..6298
                     /gene="atpD"
                     /locus_tag="U4A12_002338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN1774680.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6312..6734
                     /locus_tag="U4A12_002339"
     CDS             6312..6734
                     /locus_tag="U4A12_002339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN1774681.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         2308 a         1278 c         1544 g         1990 t
ORIGIN      
        1 tagcatttaa tctgataaaa catgattatc ataggttttt tcttagatca ggagcaccgt
       61 gtataaagaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
      121 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
      181 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
      241 gattggatga acgctcgcta acgttccaca ttggaccaat ttggtttgat ggtacagttt
      301 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
      361 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
      421 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
      481 ttacattgtt tttatttgtt tttgttgcaa acaacatcgg tttagtaaca aaaatcgttt
      541 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
      601 tggcatttat aatgatctta cttactcact tatttggggt gaaaaagtta ggcttcaaag
      661 gatatttcgt aaattcgttc ttgaaaccat atagttttat gtttccgatg aaactgattg
      721 aagaatttac aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
      781 aagttttgct aaccttgatt gcaaaaatgg taataagctt aggctggttc tcattgccgt
      841 tagcaattcc attagagatg gtctggatcg cattctcctt gtttatcgga agcatccaag
      901 catttgtctt cgttacttta tctatggttt atatgagcca taagattgaa gtggaagaat
      961 aatattcgtt ttaaaaacaa actactattt atctaattta ggaggaaaac aattatgaat
     1021 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     1081 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     1141 cgtacaacaa tgtttatcgg ggtagctcta gtcgaagctg ttcctatctt aggggtagtt
     1201 gtcgccttga ttctagtttt tggtgtttaa ttgaatcagt attaaggcag aaacaatttg
     1261 gtttgttact gcttttttac atgaaaacta gaagtacttc aaagaaagga aggctcgcct
     1321 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggaaa tgtcatcgtt
     1381 gtcagcggct catttttgat actactggct ctcttgaagc attttgcttg gggaccaatc
     1441 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttggattc tgcagaacaa
     1501 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     1561 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     1621 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gtaaagccca agctgatatc
     1681 actgtagaac gtgattcagc gctgaactct gtaaaagacg atgttgcaga actatctctt
     1741 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcacga atcattaatc
     1801 aatcaatata tcgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     1861 acgttacggg aaagctttat ttgaactggc gatcgattca aataaagcag aagaaattta
     1921 tcaagatctc gtaagcttac gtcaaatcta tgaacaagta cctggattag gtaatatgtt
     1981 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgttagtgg
     2041 ctatgatgga atcgttaaaa actttttaga agttgtttat gcgtataacc gaatggatga
     2101 tcttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag gtttgctgtt
     2161 gggaagtgtc acaacagcag ttccactttc agacgaacaa cgaatacaat tagaaaagaa
     2221 tgttgcaaaa acaatgaact atcaatcagt cgaattaaaa caaatcgttg attcttctat
     2281 tattggtggc gcgattgttg aagctaacca tcgagtaatc gacggaagca ttcgcacgca
     2341 attagaaaat atgcgtaatc aattaaatag atagtacaaa gaggtaggtg aatcgaatgg
     2401 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     2461 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     2521 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaatggc tcatacggaa
     2581 tggcgcaaaa cttagaatca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     2641 ttcgtgaagg agataaagta aaacgaacag gaaagatcat ggaagttcca gttggagagg
     2701 ccttgattgg acgggtagtt aatccgctag gtcagccaat tgacggacta ggtgaaatcg
     2761 ttactgacaa agctcgtcca gtagaagcaa tggcaccagg cgttatgcaa cgtaaatctg
     2821 ttaacgaacc aatgcaaaca ggtctaaaag caatcgacgc tcttgtacca atcggacgcg
     2881 gccaacgtga attagttatc ggtgaccgta aaacagggaa aacttccatt gcgatcgata
     2941 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     3001 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     3061 tcgttgtgaa tgccggtgcg tctcaaccag cgccgttgct ttatatcgca ccatatgctg
     3121 gtactgcaat gggtgaagag ttcatgtaca acgggaaaca tgtcttgatc atttttgatg
     3181 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag
     3241 gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag
     3301 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
     3361 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
     3421 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
     3481 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
     3541 tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
     3601 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
     3661 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
     3721 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
     3781 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
     3841 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
     3901 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
     3961 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
     4021 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
     4081 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
     4141 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
     4201 atgttgctca gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt
     4261 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
     4321 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
     4381 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
     4441 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
     4501 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
     4561 aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat
     4621 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
     4681 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
     4741 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac
     4801 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
     4861 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
     4921 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
     4981 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
     5041 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
     5101 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
     5161 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
     5221 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
     5281 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
     5341 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
     5401 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
     5461 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
     5521 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
     5581 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
     5641 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
     5701 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
     5761 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
     5821 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
     5881 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
     5941 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
     6001 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
     6061 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
     6121 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
     6181 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
     6241 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
     6301 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
     6361 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
     6421 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
     6481 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
     6541 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
     6601 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
     6661 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
     6721 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
     6781 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
     6841 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg
     6901 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
     6961 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
     7021 aataagggac tgcgacaaga cttttgggct ctttgtcaat aaggactgat gagttgtgca
     7081 aaatcaaatc tgagtcagaa tgaaccacat tctggctcag
//
LOCUS       DASJOX010000120         7046 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJOX010000120 DASJOX010000000
VERSION     DASJOX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.121"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..1248
                     /locus_tag="U4A12_002340"
     CDS             94..1248
                     /locus_tag="U4A12_002340"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN1774682.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="U4A12_002341"
     CDS             complement(1270..2022)
                     /locus_tag="U4A12_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1774683.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="U4A12_002342"
     CDS             complement(2386..5052)
                     /locus_tag="U4A12_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1774684.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="U4A12_002343"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="U4A12_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1774685.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2073 a         1455 c         1277 g         2241 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttc
//
LOCUS       DASJOX010000121         6936 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJOX010000121 DASJOX010000000
VERSION     DASJOX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.122"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U4A12_002344"
     CDS             complement(<1..182)
                     /locus_tag="U4A12_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1774686.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U4A12_002345"
     CDS             517..951
                     /locus_tag="U4A12_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1774687.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U4A12_002346"
     CDS             974..1294
                     /locus_tag="U4A12_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774688.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U4A12_002347"
     CDS             1435..1626
                     /locus_tag="U4A12_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774689.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="U4A12_002348"
     CDS             complement(1697..1954)
                     /locus_tag="U4A12_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN1774690.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1981..2124
                     /locus_tag="U4A12_002349"
     CDS             1981..2124
                     /locus_tag="U4A12_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774691.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            2112..2375
                     /locus_tag="U4A12_002350"
     CDS             2112..2375
                     /locus_tag="U4A12_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774692.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="U4A12_002351"
     CDS             2399..2587
                     /locus_tag="U4A12_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774693.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="U4A12_002352"
     CDS             complement(2670..2855)
                     /locus_tag="U4A12_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774694.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="U4A12_002353"
     CDS             3070..3441
                     /locus_tag="U4A12_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1774695.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="U4A12_002354"
     tRNA            complement(3591..3663)
                     /locus_tag="U4A12_002354"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="U4A12_002355"
     CDS             complement(3709..4137)
                     /locus_tag="U4A12_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774696.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="U4A12_002356"
     CDS             complement(4197..4955)
                     /locus_tag="U4A12_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1774697.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="U4A12_002357"
     CDS             5250..5447
                     /locus_tag="U4A12_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774698.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="U4A12_002358"
     CDS             5553..6035
                     /locus_tag="U4A12_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774699.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="U4A12_002359"
     CDS             complement(6093..6689)
                     /locus_tag="U4A12_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1774700.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2350 a         1066 c         1357 g         2163 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacagctgat atataattat taaatctcta tcttct
//
LOCUS       DASJOX010000122         6935 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJOX010000122 DASJOX010000000
VERSION     DASJOX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.123"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..1151)
                     /locus_tag="U4A12_002360"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="U4A12_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="U4A12_002361"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="U4A12_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN1774701.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="U4A12_002362"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="U4A12_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="U4A12_002363"
     CDS             complement(2435..2665)
                     /locus_tag="U4A12_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEN1774702.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="U4A12_002364"
     CDS             complement(2895..3713)
                     /locus_tag="U4A12_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1774703.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="U4A12_002365"
     CDS             3874..4563
                     /locus_tag="U4A12_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1774704.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="U4A12_002366"
     CDS             4577..6079
                     /locus_tag="U4A12_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1774705.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="U4A12_002367"
     CDS             6092..6568
                     /locus_tag="U4A12_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1774706.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="U4A12_002368"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="U4A12_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2326 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DASJOX010000123         6871 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJOX010000123 DASJOX010000000
VERSION     DASJOX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.124"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            114..389
                     /gene="rpsP"
                     /locus_tag="U4A12_002369"
     CDS             114..389
                     /gene="rpsP"
                     /locus_tag="U4A12_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN1774707.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            401..652
                     /locus_tag="U4A12_002370"
     CDS             401..652
                     /locus_tag="U4A12_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN1774708.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            777..1568
                     /locus_tag="U4A12_002371"
     CDS             777..1568
                     /locus_tag="U4A12_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774709.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1738..2259
                     /gene="rimM"
                     /locus_tag="U4A12_002372"
     CDS             1738..2259
                     /gene="rimM"
                     /locus_tag="U4A12_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN1774710.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2249..3010
                     /gene="trmD"
                     /locus_tag="U4A12_002373"
     CDS             2249..3010
                     /gene="trmD"
                     /locus_tag="U4A12_002373"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN1774711.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3146..3493
                     /gene="rplS"
                     /locus_tag="U4A12_002374"
     CDS             3146..3493
                     /gene="rplS"
                     /locus_tag="U4A12_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN1774712.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3625..4761)
                     /locus_tag="U4A12_002375"
     CDS             complement(3625..4761)
                     /locus_tag="U4A12_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1774713.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(4877..5791)
                     /locus_tag="U4A12_002376"
     CDS             complement(4877..5791)
                     /locus_tag="U4A12_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN1774714.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(5882..6310)
                     /locus_tag="U4A12_002377"
     CDS             complement(5882..6310)
                     /locus_tag="U4A12_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1774715.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(6315..6689)
                     /locus_tag="U4A12_002378"
     CDS             complement(6315..6689)
                     /locus_tag="U4A12_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1774716.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         2189 a         1200 c         1249 g         2233 t
ORIGIN      
        1 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
       61 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
      121 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
      181 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
      241 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
      301 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
      361 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
      421 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
      481 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
      541 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
      601 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
      661 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
      721 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
      781 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
      841 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
      901 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
      961 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
     1021 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
     1081 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
     1141 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
     1201 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
     1261 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
     1321 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
     1381 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
     1441 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
     1501 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
     1561 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
     1621 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
     1681 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
     1741 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
     1801 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
     1861 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
     1921 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
     1981 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
     2041 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
     2101 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
     2161 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
     2221 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
     2281 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
     2341 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
     2401 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
     2461 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
     2521 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
     2581 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
     2641 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
     2701 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
     2761 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
     2821 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
     2881 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
     2941 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
     3001 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
     3061 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
     3121 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
     3181 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
     3241 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
     3301 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
     3361 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
     3421 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
     3481 aatccgtcgt taatcatcgt tatcagactg agaaagaacc cttggtattc aagggttctt
     3541 tttttgttct ctgttttttt atattaacga tttctatttg ggacattttt gattaataaa
     3601 aaggggcaaa aaaggggcaa caagttacag tttttctata tcttctttca atttttcttt
     3661 tactcccttt gtcacatgca aataaatctt ttcagtgata tcactatttt cgtgcccaac
     3721 gcgatcctga atagcataca gaggagttcc tagttccgct aattttgaaa tatgggtatg
     3781 tctaaaaata tgtgaactaa gttttttatc aatcttcatg tcagctttat gatttctcaa
     3841 ataggtgtta attgctgtta gttggaaagg agttcctttc gttgtttgaa atagaaattg
     3901 accattcgga tttaattcta gaagctcatt gtaaatagct atcgcttttt ttggtaaatc
     3961 aatttctcgc attccagcag cagtttttgt agaatcagat tttttcatat cagctattga
     4021 acgttctcta tacatcatcg tcccatttat aactactgat gcattattat tggtaatgtg
     4081 tacatcgtct ttacttaatg cgattgcttc tccaggcctc attccagtca aatatagcca
     4141 ctggaaaagg agagaatatc ttttattgtg tgaagttgtg aaatttacta gtcttttata
     4201 ttcatcatct tctaaaaact tatctttaat ttttatcgtt tttgactctc gtttataatc
     4261 aataactact tcgttgatag gatttttttc tacatagcct tttttcatag cgtatgaaaa
     4321 aagaagattc aatttggatt taattacact gacgtactta tttgacaagt catctttata
     4381 tatcatatct tcaaaaatat tatttaaatc tactgtatta ataccagaaa cgatgtaagt
     4441 ctcaggtatc tttttcttta tagtgtttaa gatattattc gtaggataat atgtagactc
     4501 cttaacttgt cttttgtaaa taactaacca ttcttcgaca agctcatgaa atgttagatc
     4561 aggcttctga agtgtttttt cgtttaattt tatatcaatt tttttattta actctagcaa
     4621 tgccactttc tgagtttctc gagatttatt tttataagta atgctcactt tttttctttt
     4681 tctagttttt ggatcaatat atcgctcatt atatttatat actttttttc cgtttttatc
     4741 agttttggtt tcaatccaca ttgttttcat ctcctatttg ttgctataat aggaatggat
     4801 aagtaagcca attatagcag gtttattttt catcacgtcc acaaactttg gcgagggagg
     4861 gggcgtgttt ttttgtttag tagctacctt tccatgaagt atctgcgaag aacacttcaa
     4921 atgttgttcc atcatttttc acttcaaaat atgctgttcc ttgtgcggat ttacccggtt
     4981 ggatggtttc cgaaaagtaa tctctactag ctaaatcaga tttaatatct gatgaatcat
     5041 aaaattctaa ataacttgaa cttgcatcaa aaggtgtagt ccctgtgttt tctacaagaa
     5101 aatctacctt tgcgtaaaat aggccttctg gtttatagaa atcatcaccg taatcttttg
     5161 atacagactg gacagttacg ttaatagagt taccattttc agaagtaaaa ggaactgctt
     5221 ctcctatact atgtgcaact gatgccgtct ggggttctga agacgaagtt gtagttgtag
     5281 ttgtagtaga gcttgaatct ttggattttc catctttatc taagaaagtt cgttgattat
     5341 aatatacaac ctgttcagtt tttggactaa cccaaattaa cattgtttca tcatcagaat
     5401 cagttgaata agtatattgg agcatctcta atttttttat atcttttgca ctatttccat
     5461 gttcaattaa ttcagagtgc tctttataat aatctccttg tgcatccttt gaaatagata
     5521 caattaattc atcccgtgtg atagcattgt ctaattcttt gtaaacaaaa tcatttgtcc
     5581 atttacgtaa cggtgaacca agtgtctgat aaacttgagt taaatcatca ccaactttta
     5641 ttttatgaac gtctgatgaa gttagttttt ttgtttcata ttcagatgtt gatgaaacag
     5701 tattttcttt agtgctagca gaattaggac tagtggagca tccgcttaat accaagccta
     5761 aaaaaagcaa cccaaaaaca atttttttca tttttaattc ctcacttcta tgatatgatt
     5821 tttttgtagg atcttagaaa taaggttttt agtccgtgtt gcagcacgga ctttttttac
     5881 attatataag aaagcgataa gcgctttctg gaagtccgta aagattcttt aattcctcaa
     5941 ttcgtttagg atattgatca ttgtcttctt tgtatagaga aacaatgaga ttagcagcaa
     6001 agcaattagc ttcgctttca gatttgcttc tagatgttct tgttgataca tagtaactgg
     6061 ataagccacg atgaaaaata gcgtgaccta attcgtgagc gcaaatgtag aatctttcct
     6121 cagagtctct cagttcatca tttaagaaga ttattgcacg acctctaatt tcttgaaact
     6181 gtcctttggg attctcgata aaaggaacgt attgaatttt aatgcccatc ttttcacaaa
     6241 tataaaaagg attagcggac tggtatttcc gcttcaactc ctcgactaaa ttaatcgtat
     6301 ccatctccat aagctcacat ctttttgcct ttttctttgt cttctttcac aatatcccag
     6361 aaggttccaa taaggatatc ttttacgcgt tggacctgtt caggcgtcag tgtttctccg
     6421 ccataagaca tattgacatt tgaatctagt aatttatcaa gttcaactac ttcatctttt
     6481 gttgcccagt caggaacttg gtttctaccg agtaaataat cagtagttac attaaaataa
     6541 tctgctacaa gttgtagtct tttagtacta ggagttctat ttttccattg ataaatcgtg
     6601 tttttaggta tatttaattc ttcttccaat tgagttatac ttatgcctct tttatgagct
     6661 aattccttta ttctatctag taaattcatt ttcgcacctc acaagctacg aaaacactaa
     6721 taaaaaaatt agttaaaggt gttgacaact aataaaagtg ttagtatact gttttcgtaa
     6781 gctaaaatat tagctaaaaa gacttgataa ctaataaaca cttcacggtc ggcaaactct
     6841 gaaatgtaaa ttactaggca tttctgtgtc t
//
LOCUS       DASJOX010000124         6677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJOX010000124 DASJOX010000000
VERSION     DASJOX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.125"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..413
                     /locus_tag="U4A12_002379"
     CDS             <1..413
                     /locus_tag="U4A12_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774717.1"
                     /translation="KKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHA
                     MILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPL
                     EYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(539..1216)
                     /gene="nth"
                     /locus_tag="U4A12_002380"
     CDS             complement(539..1216)
                     /gene="nth"
                     /locus_tag="U4A12_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN1774718.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1223..1921)
                     /locus_tag="U4A12_002381"
     CDS             complement(1223..1921)
                     /locus_tag="U4A12_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1774719.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2189..3373
                     /locus_tag="U4A12_002382"
     CDS             2189..3373
                     /locus_tag="U4A12_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN1774720.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3379..3822)
                     /locus_tag="U4A12_002383"
     CDS             complement(3379..3822)
                     /locus_tag="U4A12_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1774721.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4048..4335)
                     /locus_tag="U4A12_002384"
     CDS             complement(4048..4335)
                     /locus_tag="U4A12_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774722.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4506..5858)
                     /locus_tag="U4A12_002385"
     CDS             complement(4506..5858)
                     /locus_tag="U4A12_002385"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN1774723.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6131..6577)
                     /locus_tag="U4A12_002386"
     CDS             complement(6131..6577)
                     /locus_tag="U4A12_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1774724.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1813 a         1313 c         1092 g         2459 t
ORIGIN      
        1 ataaaaaagc tgaaaaattt tgtttattat taaataaatt tactttaatt gctcctatcc
       61 ttgcatttat tattttttct gtacttttaa gcactactct taaaggaaaa tttatggaaa
      121 gaagctctca tgcaatgatt ctcacttttt tatggttgtt gttcaccaga atctatattt
      181 ttctgatgag cctgaaaccg cccaaatcca ttagtttatg tttagttata aatggtattt
      241 ttttattatc acttatcatt tttattacac ctttagatag atacgtcact tatttatata
      301 atcctttgga atactggact tattttatag gtatattgga aggcattatt ttctacatcg
      361 ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg taataagtta
      421 ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag
      481 cttttctcca taataacaag ataattcata catttatttg caattaaaga aaccacgtct
      541 acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt
      601 gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc
      661 taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca
      721 tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg
      781 agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata
      841 cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg
      901 gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt
      961 tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg
     1021 ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata
     1081 catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct
     1141 tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt
     1201 tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca
     1261 attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt
     1321 tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa
     1381 tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct
     1441 tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga
     1501 tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt
     1561 tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat
     1621 catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta
     1681 tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga
     1741 ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca
     1801 tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt
     1861 taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca
     1921 tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt
     1981 ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt
     2041 ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg
     2101 ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt
     2161 taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt
     2221 ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact
     2281 tccgttttta acgctgcctt attctcactc catacatcag caagctttct atatcacttt
     2341 gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag
     2401 tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata
     2461 tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg
     2521 attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa
     2581 tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg
     2641 tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc
     2701 actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat
     2761 aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca
     2821 attattcaag atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc
     2881 agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc
     2941 tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt
     3001 cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt
     3061 cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt
     3121 gtttttattt gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa
     3181 tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca
     3241 aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc
     3301 agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta
     3361 caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa
     3421 acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg
     3481 tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa
     3541 ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc
     3601 tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa
     3661 ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag
     3721 agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc
     3781 taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc
     3841 tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa
     3901 cccaatctga aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga
     3961 ttaaaagata gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag
     4021 gatgagcggc cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg
     4081 ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg
     4141 tctactgaaa ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta
     4201 atattcctct tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt
     4261 gatcgttaat ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa
     4321 atattcttta ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt
     4381 cttttatgta acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa
     4441 ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt
     4501 tttctttatt gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa
     4561 ttccatgggc ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca
     4621 gtcattttat tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg
     4681 gacatgaact gagcacctac gatacgattc gttccttttt catagactag ctccatcatt
     4741 actttttcag tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg
     4801 acttctagat cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag
     4861 ccaaataggt aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc
     4921 aagttatttc ccacaagcaa accttgacga acggcatttg ttgccaaagg aatatagttt
     4981 tgcgtgccac ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga
     5041 ttacttgtct gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt
     5101 ccttcgatca gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca
     5161 aatgtatctg ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca
     5221 gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata
     5281 tctgtgaatg gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc
     5341 acttgttttc cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca
     5401 acaattacta cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag
     5461 tttttgcata ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat
     5521 gaaccagttg tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga
     5581 tcttttgccg ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta
     5641 actgttgctc ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc
     5701 tttactactc cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt
     5761 ctttcatata ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca
     5821 gtacctgcat gagtacagcc aattactact actttcattt atggtccctc cgattaacat
     5881 tttgtccttc gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg
     5941 tgaattcaca aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct
     6001 acaagattct tattttaata atattcactt ttatatctaa gattgtgaat aaaagacaaa
     6061 aaaataaaaa agctagcgtt ttttttacac agctagcttt ttttaggtat aacacttgta
     6121 gaaactgttt ttacatttgc gccttttttg ctgactcttt acaatccgga cagagtccat
     6181 acaattccaa ccgatgttca ttaatctcaa aatcagtcag tctgcttgca gccatttcta
     6241 tatcttcaag ccctgggtaa tgaaaatcaa caatcttacc gcagttttca caaattacat
     6301 gataatgacg ctttgaactg aaatcaaatc ggctagaagc atcgccgtac ttcatttctt
     6361 gaacaaatcc aatcgaagta aataagcgaa ggttattgta aacagtcgca acactcatat
     6421 ttggaaaccg atgttcaagt gctttataaa tctcatcagc cgttggatgg gaatgatgtt
     6481 caatcaaata ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta
     6541 acttttcgat aacctcttcg actaacttat tttccatgta aacaccccac gttgttattc
     6601 tcgtcttatc atactatttt tacattttaa aatccactta acatgaaaag aaaattcaat
     6661 tttattgtat tttaatg
//
LOCUS       DASJOX010000125         6630 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJOX010000125 DASJOX010000000
VERSION     DASJOX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.126"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            146..2821
                     /locus_tag="U4A12_002387"
     CDS             146..2821
                     /locus_tag="U4A12_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEN1774725.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3003..3242
                     /locus_tag="U4A12_002388"
     CDS             3003..3242
                     /locus_tag="U4A12_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774726.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3258..4631
                     /locus_tag="U4A12_002389"
     CDS             3258..4631
                     /locus_tag="U4A12_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1774727.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4635..6269
                     /locus_tag="U4A12_002390"
     CDS             4635..6269
                     /locus_tag="U4A12_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1774728.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6432..>6630
                     /locus_tag="U4A12_002391"
     CDS             6432..>6630
                     /locus_tag="U4A12_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774729.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2323 a         1071 c         1331 g         1905 t
ORIGIN      
        1 gtgatttcca aaatcacaac ttttttcttt tgtcaagatt ttgagtggga tatatttttg
       61 tagaactgaa gatcatgaaa ttatgagagg ttggaggtaa tgtagtcata caaataaaaa
      121 ttttttagaa agtgagtggt aattaatgaa aatgaatcac acttcaattc agaagcattt
      181 acaactgtta aatacactgg ggatttctta tgttaatttg agaacttttt ctacattaaa
      241 caagaaaacc gtttctcata ttttttctac tgcagattct caagcaatta ttaactattt
      301 agaaaaacag aaattgtctg aaggtacagt aaacataact tataatccta ttaaacaaga
      361 ggtgattaca caaaaaaagc attctgtgag agacaatgat attgaaaaaa ttcggtttgt
      421 atttattgat attgacccta aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa
      481 ggcagaaaat gttatggaac aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt
      541 tgtaaaagtt gatagtggga atggctacca tatttttctt cccataaatg aacaaccaaa
      601 taatcacgag acgattctta ctattcagaa ttttcttcaa ttacttcacc gaagatttgg
      661 agtagaagat gaagttgata ttgatacttc tgtttataat ccttcaagat tatgcaaatt
      721 agttggtaca ccagcggtca aaggaaagtc cacaaaagag cgtccgcatc gtttatctag
      781 tttcctttct attcccgata acatagatag aagtaattgt tttaaaattc ttgaacagat
      841 agttgaagaa aattccatga tgtcaacctc tagagtaaaa agccatccat cttttagaga
      901 agtgttacct tttattcaag cagactataa acaatggtta gctcactatc ctgaattaac
      961 ttataccata aaagagggcg atcataaagg agttacctta cttatatttg acgaatgtcc
     1021 attaagaaaa cacacgaata attctaatgg ttcttgtttg tctgttacgg ctgataataa
     1081 gattcgattt cattgtctgc atgcttcgga taaaaataaa gatattcaag actttgtaaa
     1141 aaaatatccg ttgccaaaac ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga
     1201 atattatttt gaccagttta aactgaatca agaaggagta tttttggtga aagaaggtca
     1261 agaagaaaaa gttgcttgtc cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa
     1321 agtcattaca cttgttcttg cctattatgt cgatcattgt tgggatgaaa tagaaataga
     1381 aggtggatgt ttacaaacta ataattttaa acaattagca aaacatggct taatcattaa
     1441 agctagaaga gaagctgaag tcgttgactt tttacagatt caacgggaga atgctccaat
     1501 cttttttgaa catgaatctc ttggctggaa aaaagaaaat gaagcaatga tttttcagtt
     1561 acaagattct tttggacgtg aaattccttc tgtattaact aaaaactcat gctaccaatt
     1621 aacaactaat ggtacagaag cggaatttga cgcattggtt cataagtatg ttttaggaac
     1681 gaacttagaa cttgcttggt cgattggtat tgcttctatt gtgttaggct atttaaacgt
     1741 tacgaaggct aatacttttt tgagtttact aattaaccta cagggcaaga gcacaacggg
     1801 taaaaccact acgttacact ttattgcctc tttatttgga aatccaacta cggttcttag
     1861 aaatatgaat gctaccaata aagccattat aaagctagca tcaaacaatc agggaggtgt
     1921 tccactaata ctagatgagt taggagcagc aactcaaaat aactttagct cgttgatcta
     1981 tcaattagct tcaggagaag aaaggctaag attagattct aattgtcagc ttatgccaca
     2041 agagactttt tctgttttga cccttacctc ttctgaagag cgtttggaaa attatttcaa
     2101 tgagaaatta actggtttga aagttcgata tatcgaattt tctgatatca agtttacaaa
     2161 ggatgcgaat cacgcagaga aagttaaaag tctttcttct aatacttttg gttggggtat
     2221 aaagttattt cttttgaaac tctttgaaca agggattaca aaaattgaag caacttttgt
     2281 cgaagctcaa aaaaaactat gggctgtttt acctgaacat tctttgaagc aacgcattgg
     2341 taataatcta gctttgattt atagtggagc cctcctaatt caagatatac tgggctatcc
     2401 tttacgatta gagcatatac agacggtgct tattaattcg tacgaacagt taattgtaga
     2461 tactagttta ggaagtgagc aaacggtatt tgaccgagtg aaaacattgt tggtgatgaa
     2521 tagccataaa tttttaacaa acagtgacaa caaacatacg cccagtacga tttggggaaa
     2581 agtatcaatt caaaaaaatg gggatattaa ggtaaatatt tttcctatcc cgtttgagga
     2641 cttgttaagg cgagaatttc aagtaaagga tctaacctat ctttttaggc aattgcttga
     2701 ttcgggtaat atgtgtgcag aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca
     2761 agttcttcca acatacgaga ttctattacc ttcttcactc aaatcgtatt ttcgattgta
     2821 atttagaata aaccaaagta tgaaagtgag gcaccatgtt atggaaatat ttcttattga
     2881 tttagcaaaa caactgatta cttatctgta tgatagcgag caaacccaaa cggaagtaaa
     2941 tgaagcttga tctatgcttc atttacttca ttcaatcaaa catgaaagaa aggaaaattc
     3001 taatgactaa aaataaaatg acactaaaag cagaggttct tctgtatatt caagaacatt
     3061 tttcaaatca agcctttttt acaaaaccaa tttacttgga tttcgaaata agaggggtat
     3121 ctgctggatc tatcgggggt actcttcaag cgttgaagaa cgaaggatat ttagaaaatc
     3181 actttgtgca acgttcgttc aatagaagag tcgtgaaaaa atggtattta gttcattcat
     3241 agagagggga atcaagcatg cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag
     3301 cgaaaattag taatgacgct caatatgatg cagtgaaaga gtatgtttta gagaaatatg
     3361 aacaaattcc ccaacgattt tcagacaaag gagaatcagg ggaaaaagcg gagcgtccag
     3421 cttttcgaca gcttagagct tgggtttgtg cgtcagaaga agagggggtg atctatgttt
     3481 ggcgttatga tcgattggca cgtcaagcca aaatagcctt agaatttatt gagttgtgcc
     3541 aacaacatca ggttcaagtg tattcgattt ctgaacctct tcctgcttct ttctcacacg
     3601 cgtcaacgaa taaactattt gtgcaattat tgttcacttt tgcagaacaa caacgaaacg
     3661 tcattattga gaatgttcgt gccggacttt cctataaaaa gcaacaacgg caatatttgt
     3721 cttctgcggt accatatggg tatcgtttag tgaatgggaa aatccaacaa gaagtacacg
     3781 aagcaaggat tgttcgctat atttttcaac tttatttgac caaaaaatat ggctacaaaa
     3841 agctttgtca acgattgacg caacaaaagc ttttcttccg cgaacgtcct tttcagccct
     3901 atcatattta tagtatccta aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt
     3961 tggggaagta tctaggaacc tttgaaccga ttctctcgaa agcaaccttt ttacaagccc
     4021 aagagattcg ccaatcaagg tgtacagcta aaaaagatac gtatctgtac ttattacgtc
     4081 aaaaaattcg gtgtcccttc tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa
     4141 agaagacgaa gacgcttcat tattatcatt gtaccgaccg aagctgccaa ggaatttttc
     4201 ttcgggcaga aagggttgag tcagaagtat tggaaaagat acaggctttt tttcaatacg
     4261 aaggcgtcta tcaagccttg attgcccaat taacgcaaca attagccgaa aagcgaaaag
     4321 atgaaaaacg caaagtaatc caacaaaaga aacaagaagc acaatggatt aaggcatttg
     4381 aagagggcaa ttgttcactg gaagaactca agcaacaact gcaagctctt caaaagccga
     4441 tgtctacttc ttcgtttctt tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc
     4501 aactaaaaga cctctctagt cgacaattat tatgtgacta tgtttgtcgg attgaggtaa
     4561 caaaggataa aacagtttgt gggatttact tccgacaact gacacaaaat attttagaaa
     4621 ggaacgaatg atctatggaa atgatacaaa ccaaacgagt cgcattgtat attcgcgtga
     4681 gtacacaaga acaagcttta gaaggacata gtattcaagc acaagaacaa aatggacgac
     4741 agtttgccga acggatgggc tatgaagtgg tagaagttta tgccgatgaa ggaatcagtg
     4801 ggaaatccac aaaaaatcgg ttcgcctatc aacgaatgat gaacgatgct cggaaccatc
     4861 gttttgatct actagtgatt tggaaattga cccgtctcgg acgcaatatg ttagatgtct
     4921 tacaaacagt cgaggaatta ttgcgatatg acgtaggatt acattcgatc tcggaacaat
     4981 ttgacgtgac cacttcgaca ggtaaattga tgttacaact ccttgggagt ttcggtgaat
     5041 ttgaacgcaa ccaaattgcg gagaatgttc aaatgtcaat gcaaagctta gtgaaagata
     5101 aaaagcgtta tgcaggaggc cgacgtttag gctatgtcag tggtattgac acgaatggtc
     5161 aaaaacaatt ggtcattgaa ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat
     5221 atttaggcgg agcagggtat cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa
     5281 cagtcaaaaa gaatgctttt tctaccacgg cagtcaaaga tatcttacgc aataaaattt
     5341 atgcagggta tttagaatat gcgcgctaca tcaattggga aaccaaacga cggaagggga
     5401 agaatccgag accgatttta gtgaaaggaa cccatgaacc gattatttcg gaagaagatt
     5461 atcaaaaagt acaagaacgc cttgctttag aaggaaaaca acccaaatgg aaccatacag
     5521 gtgaaaatgt attgacaggc ttgttacgtt gtccagaatg tggcgcaccg atggcagcga
     5581 gtaatgtcac aaatacccta aaggatggca caaagaaacg gattcgatat tattcttgtt
     5641 cagtctttcg gaataaagga gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag
     5701 cggaaacatt tgtcgccgac cgattaaagg agatcgttca gctaccagaa gttcttcccc
     5761 gattggtggc agcgttaaat gaagaaatag tgcgacaaag ccaaccattg gaacaagaac
     5821 tcgtggtgtt actggaacga aaggaagaat tgaagactaa aattgaaaaa tgggaagctg
     5881 ctttggaaga tagtcctgaa ttgtttccga tgttaaaaga ccgcttagac gaattgactg
     5941 aaaaacgacg acagttgcac attcgagaaa atgaaatctt agggatcttt caacaaggag
     6001 agccgattca agtcaaagat gtccaacgga tattgactaa tttggatcgt tttcttgccc
     6061 agagtgagaa gaagcaaatc aaagccttgt atcgtacctt tatagagaaa ataacctttg
     6121 atcctaacca taaggagatg ttggaaatta cgatgaaatt cacgcatgcc gtggtacaac
     6181 aattgaatga gcaatatcaa atagccgtat ccaaaacaaa ggatacggct atttttgtgt
     6241 taaaaacacc ctttacgtta acgatctagt ctaggttata tcaaacaacg ttagaggggc
     6301 gctaacgctt ctctagcgat ttgaaatcgt tagagaagta gttagggagg tccttatcat
     6361 aaatagggat atttattaat gaatatttga aatataattg tatataagaa ttaaaaagag
     6421 tggggataga tatgaaaaag atgggtaagt gtattttatg tgaaaaatac actgaactta
     6481 ctaaagaaca tgtaccacct aaaaaatcag ggaatactgg aggtaaaaag accagaactg
     6541 gtaatttaga tgatttcctt aagagcgatt ttacaaaagg tgattttccg aaggggatta
     6601 aaaggaaacc tcaaggaaat gtttattata
//
LOCUS       DASJOX010000126         6564 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJOX010000126 DASJOX010000000
VERSION     DASJOX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.127"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..1164)
                     /locus_tag="U4A12_002392"
     CDS             complement(7..1164)
                     /locus_tag="U4A12_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774730.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="U4A12_002393"
     CDS             complement(1167..1523)
                     /locus_tag="U4A12_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774731.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="U4A12_002394"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="U4A12_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN1774732.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="U4A12_002395"
     CDS             complement(2991..3830)
                     /locus_tag="U4A12_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1774733.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="U4A12_002396"
     CDS             4015..4902
                     /locus_tag="U4A12_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1774734.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="U4A12_002397"
     CDS             complement(5486..6181)
                     /locus_tag="U4A12_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN1774735.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="U4A12_002398"
     CDS             complement(6165..6563)
                     /locus_tag="U4A12_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1774736.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1300 c          966 g         2233 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catc
//
LOCUS       DASJOX010000127         6526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJOX010000127 DASJOX010000000
VERSION     DASJOX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.128"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1170)
                     /locus_tag="U4A12_002399"
     CDS             complement(<1..1170)
                     /locus_tag="U4A12_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774737.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKT"
     gene            complement(1181..1849)
                     /locus_tag="U4A12_002400"
     CDS             complement(1181..1849)
                     /locus_tag="U4A12_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1774738.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1979..2563)
                     /locus_tag="U4A12_002401"
     CDS             complement(1979..2563)
                     /locus_tag="U4A12_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1774739.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2894..3127)
                     /locus_tag="U4A12_002402"
     CDS             complement(2894..3127)
                     /locus_tag="U4A12_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN1774740.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3142..4092)
                     /locus_tag="U4A12_002403"
     CDS             complement(3142..4092)
                     /locus_tag="U4A12_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1774741.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4089..4931)
                     /locus_tag="U4A12_002404"
     CDS             complement(4089..4931)
                     /locus_tag="U4A12_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1774742.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4933..5652)
                     /locus_tag="U4A12_002405"
     CDS             complement(4933..5652)
                     /locus_tag="U4A12_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1774743.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6344..6502
                     /locus_tag="U4A12_002406"
     CDS             6344..6502
                     /locus_tag="U4A12_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774744.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT         2217 a         1152 c          833 g         2324 t
ORIGIN      
        1 agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc
       61 aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg
      121 atctttacta actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa
      181 ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat
      241 tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc
      301 cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc
      361 ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc
      421 gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt
      481 ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac
      541 ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc
      601 tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt
      661 tgaagacggt tcatccaata taattaattt agagtttagc gataaactac atccaataga
      721 aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag
      781 atctaataat tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa
      841 gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg
      901 atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga
      961 gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc
     1021 attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag
     1081 actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata
     1141 tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga
     1201 aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt
     1261 aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt
     1321 cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag
     1381 gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt
     1441 cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg
     1501 gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca
     1561 accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa
     1621 atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg
     1681 aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg
     1741 tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg
     1801 gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc
     1861 caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac
     1921 agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt
     1981 aactcagtaa ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca
     2041 tagtcttttc ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt
     2101 aaatgtagaa gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag
     2161 tctttaactt atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa
     2221 caatatcttg atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat
     2281 tctcaaaaat ataattaata aatggatcta attcattgaa gttctctctc caacttttct
     2341 ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa
     2401 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
     2461 gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt
     2521 gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta
     2581 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actttattat
     2641 cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc
     2701 taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag
     2761 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
     2821 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
     2881 aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat
     2941 cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac
     3001 ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt
     3061 ttctttttga taattcctga attgctagta aataaattag tatagtagtc gttttaattt
     3121 gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca
     3181 tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa
     3241 aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc
     3301 cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg
     3361 ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg
     3421 cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt
     3481 atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc
     3541 aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa
     3601 ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg
     3661 atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc
     3721 tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc
     3781 atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt
     3841 ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact
     3901 gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt
     3961 aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga
     4021 acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca
     4081 ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga
     4141 aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta
     4201 aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata
     4261 aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac
     4321 attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat
     4381 agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat
     4441 ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa
     4501 accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat
     4561 aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct
     4621 aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt
     4681 ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta
     4741 atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc
     4801 ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata
     4861 actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa
     4921 ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa
     4981 attttcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
     5041 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
     5101 ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc
     5161 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
     5221 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
     5281 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
     5341 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
     5401 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
     5461 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
     5521 aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag caccttcttg
     5581 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
     5641 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
     5701 aaaaattata ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag
     5761 attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa
     5821 aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa
     5881 attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa
     5941 attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac
     6001 ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa
     6061 actatttttg atcattgaca attaaataca atttttgaat aacgatccct gctattcgag
     6121 ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt
     6181 tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc
     6241 cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat
     6301 aactactatt ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac
     6361 ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta tatataattt
     6421 cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat
     6481 gaatacattt aaaataactt gaaattttct tctcgccgca tatact
//
LOCUS       DASJOX010000128         6525 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJOX010000128 DASJOX010000000
VERSION     DASJOX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.129"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..778
                     /locus_tag="U4A12_002407"
     CDS             41..778
                     /locus_tag="U4A12_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1774745.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            792..1607
                     /locus_tag="U4A12_002408"
     CDS             792..1607
                     /locus_tag="U4A12_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1774746.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1620..2267
                     /locus_tag="U4A12_002409"
     CDS             1620..2267
                     /locus_tag="U4A12_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1774747.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2283..2939
                     /locus_tag="U4A12_002410"
     CDS             2283..2939
                     /locus_tag="U4A12_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1774748.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3101..3922
                     /gene="racE"
                     /locus_tag="U4A12_002411"
     CDS             3101..3922
                     /gene="racE"
                     /locus_tag="U4A12_002411"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN1774749.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3925..5280
                     /gene="rph"
                     /locus_tag="U4A12_002412"
     CDS             3925..5280
                     /gene="rph"
                     /locus_tag="U4A12_002412"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN1774750.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5281..5799
                     /locus_tag="U4A12_002413"
     CDS             5281..5799
                     /locus_tag="U4A12_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1774751.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5906..6406
                     /locus_tag="U4A12_002414"
     CDS             5906..6406
                     /locus_tag="U4A12_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1774752.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2150 a         1095 c         1442 g         1838 t
ORIGIN      
        1 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
       61 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
      121 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
      181 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
      241 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
      301 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
      361 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
      421 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
      481 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
      541 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
      601 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
      661 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
      721 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
      781 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
      841 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
      901 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
      961 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
     1021 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
     1081 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
     1141 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
     1201 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
     1261 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
     1321 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
     1381 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
     1441 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
     1501 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
     1561 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
     1621 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
     1681 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
     1741 aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa ttttttagaa
     1801 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
     1861 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
     1921 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
     1981 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
     2041 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
     2101 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
     2161 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
     2221 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
     2281 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
     2341 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
     2401 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
     2461 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
     2521 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
     2581 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
     2641 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
     2701 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
     2761 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
     2821 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
     2881 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
     2941 agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc tctttccatt
     3001 cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat tcacgctaaa
     3061 aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat tgacagataa
     3121 tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa aagaagccct
     3181 gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct gcccatatgg
     3241 ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt atctggtgga
     3301 gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg ctttagaaga
     3361 aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta ctagagcggc
     3421 agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta cggtaaaaag
     3481 tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga caagtcttgc
     3541 ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg tggcgaaaaa
     3601 aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat tgattttggg
     3661 atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag aaaatgttca
     3721 actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag attatttcaa
     3781 tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa ctggctctgc
     3841 caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa atgtagaaca
     3901 tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca agagcttcgc
     3961 aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt gatccgtttt
     4021 ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc ttttttgaga
     4081 gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc aacgagtacg
     4141 agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga aatccagcgt
     4201 cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga acgtagtatc
     4261 atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag tatcactggg
     4321 gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga attgtcagaa
     4381 gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga tgggacttgc
     4441 gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa tcttgtaatg
     4501 acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac gtttgatggt
     4561 gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt gatcaaagag
     4621 caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga agaaacaaaa
     4681 acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa tatgttcaaa
     4741 gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga tgtggaagaa
     4801 actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc acaattgctt
     4861 gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg cggtatgcca
     4921 ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa taacgccaaa
     4981 ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt ccattgtacg
     5041 ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg gcccggtcga
     5101 gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt tatcccagaa
     5161 ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat cagtcatcgg
     5221 gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg agagagatag
     5281 atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt tgacttagta
     5341 gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga attagaagct
     5401 tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta tggaccagga
     5461 ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac tcacggtcat
     5521 ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc tgcaactgcc
     5581 gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca gaaaatcctg
     5641 tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc atcatatgct
     5701 cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc ccatcaggca
     5761 gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact ctaattttta
     5821 ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata gtagtgttga
     5881 atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa tgctgctgga
     5941 aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt atcatcatcc
     6001 attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc ctgtattagg
     6061 aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata aaatgattga
     6121 cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg tgacagaaac
     6181 agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt tgttagtcga
     6241 ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca ttacaagaaa
     6301 agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta atatattgtc
     6361 tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa cgagaaacta
     6421 aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt atcttgaaaa
     6481 agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaat
//
LOCUS       DASJOX010000129         6482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJOX010000129 DASJOX010000000
VERSION     DASJOX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.130"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="U4A12_002415"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="U4A12_002415"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN1774753.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="U4A12_002416"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="U4A12_002416"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN1774754.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="U4A12_002417"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="U4A12_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN1774755.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="U4A12_002418"
     CDS             complement(4503..5270)
                     /locus_tag="U4A12_002418"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN1774756.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="U4A12_002419"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="U4A12_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN1774757.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         1654 a         1430 c         1141 g         2257 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccgatgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 ta
//
LOCUS       DASJOX010000130         6429 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJOX010000130 DASJOX010000000
VERSION     DASJOX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.131"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..438
                     /locus_tag="U4A12_002420"
     CDS             <1..438
                     /locus_tag="U4A12_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774758.1"
                     /translation="LSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLT
                     KNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQ
                     NHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            452..1141
                     /locus_tag="U4A12_002421"
     CDS             452..1141
                     /locus_tag="U4A12_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774759.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1266..3617
                     /locus_tag="U4A12_002422"
     CDS             1266..3617
                     /locus_tag="U4A12_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN1774760.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3697..4092
                     /locus_tag="U4A12_002423"
     CDS             3697..4092
                     /locus_tag="U4A12_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774761.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4417..5034
                     /locus_tag="U4A12_002424"
     CDS             4417..5034
                     /locus_tag="U4A12_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1774762.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5075..5374
                     /locus_tag="U4A12_002425"
     CDS             5075..5374
                     /locus_tag="U4A12_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN1774763.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5408..5575
                     /locus_tag="U4A12_002426"
     CDS             5408..5575
                     /locus_tag="U4A12_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1774764.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5620..6225)
                     /locus_tag="U4A12_002427"
     CDS             complement(5620..6225)
                     /locus_tag="U4A12_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1774765.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2213 a          891 c         1267 g         2058 t
ORIGIN      
        1 ctaagtattt tttcagtatt tacaatatta attgggggct attttctttt gaatttagat
       61 tctgttaatt caaacgctca aataaaagat caaagagtag ctgaacataa taataaaaaa
      121 atgagtttga ccaaaaatat ctattctatg tcagatgaag aattgtataa agaatttggt
      181 gatttatccg gggaacagtt agaagaaagt attccaggaa ttagtgactc agataaaatt
      241 gttgtcttgt ctgctggtgg gcaattaatg ggagatggca aatccgaatt agttagttat
      301 aaacaaaatc acagacagtt tattgatgtt aatactgatt ccaacgctat ttcgattaag
      361 gatctaaaag agcttttaaa tagtaaaaga aatgaattaa agcagataaa agaaactaat
      421 tcagtggaat tagagtaaag gaagattcat tatgaaaaag atttttggtg tttctgttgg
      481 gctacttatg atgttaacat ttggaaatgc agtatatgct gctgagaaag aagttatttc
      541 agatccagag cagtacgcct cacgatactt tgctgatttt gaagaagaag ataaagtact
      601 tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa attgtatcta tggataatcc
      661 agatataatt caaggttcat ataattcaga aactgatcct gcttcagttt cagtgacaga
      721 agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt gaaactactc caattatgcc
      781 tcgaggagca tttccacccg atgttggtac tagcggacac cttcttgtac tagcaggagg
      841 aggaagctat aaatcacagc catttagtgg tgcgggatgg cgttttggag gtatgggtgt
      901 aaaaccagct gatggtacag gcaacttttt acgttggaaa tcctacattg acgatggaag
      961 agtaggaaat gtaaatcaag ccaacgcaac taaatctggt tctattcaag gaatggcatt
     1021 gtatgctcgt gaaggattca aatactttgc accaaacggg cctggttgga caatgaatat
     1081 aggtatttat tacacttata atcctgttgc aggtacgtat tatgttgttc aaaatattta
     1141 ataaaacagc acaaagagat ttctttgtgc tgttttatta tttttagttg ttgacaaaca
     1201 ctaaaatata aaatataatc aacacataaa cgaacgtatg ttcggcaaga aagcagggga
     1261 tttttatgga tgagatggtt ttaagtactc agaaatggct aaacaagaaa tacagtaatg
     1321 ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg gtggcctaca atctatggac
     1381 ttattgaagg actacaagta gaacttggta ttactaatct agtagctaat tttggaccta
     1441 ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg gggcaaaaat ctacctaaaa
     1501 atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg gattaatcca ggtggcttcg
     1561 atggggtata tactccatat ttagatactg ctgtaaaaga gttgcagact gatgcagggt
     1621 ttaatggaag cgctgtaact ggagttttgg atgcgaaatg ggccaaagcg ttatttgata
     1681 tgagtgcttt tgttcttgtt cccggcggag atagtaaaat tagaaaaatg cagcaatggt
     1741 taaatgtaaa ttatttagaa tacacgggaa taatgccttg tgatggtatt tatcaaagaa
     1801 attcaaatca agcattaatt tttgcattac aagctgaact aggatattct cccagtgaag
     1861 caacagggtc ttatggtaat gggacaacta gtaaaactta tcctgtgtct gaaggtaact
     1921 cgggaaacta tgtaagaata attcaatatg gattatatgt taatggttat tttgaagacg
     1981 gtacattcga tggtattttc actaaatata tgggtttaga agtactggca ttccgaaaat
     2041 ttatggtatt gcctgaatat actgaaatag cagatgttgt tgtaataaaa ggactattgt
     2101 ctagcgctgg agatattaga agatctgcag atggcgcaga tacttctacg caattaacta
     2161 gatctcaaat ccaaacattg gttgataatg atataaaaat agttggtaga tatcttactg
     2221 gtactgttgg aattggaaaa gatgaacgaa ataagtatct aactacagag gaattgaata
     2281 atattttttc taaaaattta tctgtgtttc ctatctatca agatggaggt gcagcattag
     2341 cttattttac ttatgatcgt ggcttatcag atggtaagaa ggctatagat gcagcaaaaa
     2401 atctaaatat accattaaca acagttattt attttgctgt tgatctagac atgttagggg
     2461 aagaaattct tgcatatgct ggagaatatt ttaagggggt atctgctgta atgtcatata
     2521 gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg ttctcaaata atcaataacg
     2581 ggtatgcttc gtttgcattt gtttcagata tgtcgactgg ttatagtggg aatttaggtt
     2641 ttcctatgcc tagacaatgg tcttttgatc aatttgttga atttaccata ggaagtggca
     2701 acggagctgt tggtatcgat ttaatcgcta catcgggtag agatagcgga tttaatgagt
     2761 tatcaaatga tactaataat gataattaca ttgccaaata caatcagaag gttataaatc
     2821 agatggtacg tgcgtatcaa tatctatcac aagctggtct tgataatcct tggaaccctc
     2881 atttgacatt ttatagatat gtaaattatt ctggtttgtc atgggacatc atttcttctc
     2941 ctgtaacaga gcatgataga aaaatttatg atgaatatag gaataaatta actaatagcg
     3001 agggcctgta taattatttt atagatccta atactggttc aataataggt ttgcctcact
     3061 taattgtgac gttacaatca cagatgtttc tagccgacac aatcaatgat agtgtttctg
     3121 actttgcagg ttggttagga gatttaatga cttgctgggg tgaagttaag aaattaggaa
     3181 tacaaatgga gcaaggtgta tttgcattag taggtacggc tgcaggtgga acatttagtc
     3241 tagaagattt ataccaagat gttgacgcgg taaatgttta taccttagca aaaagaaatt
     3301 ctgattcaac agctttatcg gctttaaaaa cgtactattg tgataagggt gctattgata
     3361 gatttaatca atttgttaag aacagatttg gttcttcaga taaaatcgct tcagtaacaa
     3421 atagtttatt agaaaatgac gaaacagttg atatcttagg tgtagggata atttcatttt
     3481 tcattgataa aatagttaat aattcacttg ggacagattt tacttatctt gattttattg
     3541 gagatgaggg gaaagttgca aatggatttt ctgaaaaaat tattcaattt caagaaaata
     3601 gaagtaaact taattaagaa tcgtttacta tttaaacagt gtccttttcc tttttaaata
     3661 tatacgataa aataagtata tgaattgtaa aaggacatga tgattatgga agaaatagaa
     3721 tggaataatc tgactcaaaa taataaaaat aattctggat gtggaattat ctttatgttt
     3781 gttatagttt tgggaatagt agtactttta ataactgtca agatagtaga ttactttgcg
     3841 aatactcctg aagaacaaat acagagaaaa cttcctgagt ttgaaagagt accaccttat
     3901 gatccaagag ataattcatt tcaagtaaaa aaattaaaag agtctccaaa tgaaatgtgg
     3961 tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa agaaagaata caattatatt
     4021 gtaaaggcaa aagtatcaaa aagatttatt tttaacccta aattagaaat atatagtgtt
     4081 gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa gaagtcaaat tctgatgtga
     4141 aaaataaaat ataccatgga agacataaaa gaaaaactgg ataactagtt tatttaatgg
     4201 cagaccgtta aggcctgcca tttttatgta ttttagcgga ataaatataa tcatgcggga
     4261 gtgtaaactt actgtttcaa aaagtatact ttttgacatg ccttgatttt ctttgtttat
     4321 gtatggcata ataaccacat aatacaattt gaaacgtttc tgatttaatc tgattgtttt
     4381 gaaacaattt tagtgaaaaa agggtagata aaaacgatga gaaaattttt ttatatccgg
     4441 gtcagtagtt atgatcaatc gatcgaacga caactgattt ccgcgaagga attaggaatc
     4501 cctgaagaat atatatttgt agaaaaagcc agcggcaaag attttaaacg ccctgaatat
     4561 caattaatga aacggatgtt tcgaaaagga gatttactct atctacaatc attagataga
     4621 ctaggcagaa ataaacaaat gattttagaa gaatggcgag aattaactaa ggaaaagcag
     4681 attgatattt ctgtgttaga catgcctctg ttagatactg ctagttacaa gcaactagat
     4741 gggttagaga cgcttatctc tgacttaatt ctgcaattac tcagctatat ggccgaagac
     4801 gagcggaaac gaattaatag ccggcaaaga gaaggcgtag aagctgcgaa acaaaagggt
     4861 gtaaaattcg gcagaaagaa aattgcttta cctcctgaat ttcctgcgat ctattctgac
     4921 tggaaagcag gaaagataac tgcagtctat gctatggatc aattgggcat gaaacgaaat
     4981 accttttatc gacgggtgaa agaatatgaa cataccttgc accacttttc ttgatagata
     5041 taacagctat tagacgggaa gggagagttt gaaaatggac ttaacgaatc aaagggtcgt
     5101 ttttactgga acgctgcagc aaatgacacg aacccaagcc atcggtttgg ttcactcttt
     5161 aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat ttcctggttt gtggtcagta
     5221 ttctaagtcg ttgtttgatg attctctcta tacaaaaaag gaacaaaccg cgcgggactt
     5281 gattgcgtta ggtcatgaga ttaccatttt atcagaagtc gaattttatg cgttaattag
     5341 tcaacagttg aaagaatggc aacggtattt ataatcgtta gtaaaattta caaggaggaa
     5401 aatttttatg aagcgaaaat cttatgatcg ttggggcatt gaaataacta gttttaatcg
     5461 aaatattgct tttcacgtgg agtgtgactg tggggaatta gcaaaaagtg tttatcaaaa
     5521 aaattacttt tactgtccta aatgccatcg ccgatacggg gtacatggaa catagaaacg
     5581 aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc tacaaatcat tcgctttctt
     5641 tattttataa atagttggtc gagagatccc gttttctttt gcaatctctg caactggaat
     5701 gccttgcttt aacatgtcca ccacggtttt aaaaactaag cgtttttgcg gatctgttga
     5761 gttaatagaa tactcgattg gtcgtccctt atatcggccg gctttttgag ccaataatac
     5821 tccttgccgc tgacgttcac gaattttctc acgctcgttt tgtgcaatat aacttagcag
     5881 tgataaaaac atatcgaaca tagcggtatc caggagttca tttccagtat taaaatctaa
     5941 aaactgtgca tctaatattt ttaacgctac ttttttctgt ttcataaaag caactgtatt
     6001 ttttatatct tcataatctc ttcctaaacg atctagtgat gtaacgatga cgcaatcacc
     6061 ttcacggata taatttagta atttttgaaa ctcaggacgc tcagtatctt taccactggc
     6121 tttttcttgg aataatttat caatgttaag tccttccatc attgccaatt gtcgatccaa
     6181 attttgatct tgtgaagata ctcgaatata gccaattttt cccataaaaa actccttttt
     6241 aaaagtgata taatttacat gtaaaattta cttgtagttt tactgctatc atagcatttt
     6301 taaaaataaa ttttatgtag acttaaaatt tacttcatgg agggtttaaa tggatgaaat
     6361 caaaagtaac aaaaaagcat ttggcattta tttaggagaa cctagtacag aacaattaaa
     6421 ccaattttt
//
LOCUS       DASJOX010000131         6303 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJOX010000131 DASJOX010000000
VERSION     DASJOX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.132"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            334..714
                     /locus_tag="U4A12_002428"
     CDS             334..714
                     /locus_tag="U4A12_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1774766.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="U4A12_002429"
     CDS             696..1610
                     /locus_tag="U4A12_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1774767.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="U4A12_002430"
     CDS             1855..2544
                     /locus_tag="U4A12_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774768.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="U4A12_002431"
     CDS             complement(2665..2751)
                     /locus_tag="U4A12_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1774769.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="U4A12_002432"
     CDS             3427..4377
                     /locus_tag="U4A12_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN1774770.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="U4A12_002433"
     CDS             4398..4931
                     /locus_tag="U4A12_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1774771.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="U4A12_002434"
     CDS             4947..5117
                     /locus_tag="U4A12_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774772.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="U4A12_002435"
     CDS             5567..6100
                     /locus_tag="U4A12_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774773.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DASJOX010000132         6285 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJOX010000132 DASJOX010000000
VERSION     DASJOX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.133"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..177
                     /locus_tag="U4A12_002436"
     CDS             <1..177
                     /locus_tag="U4A12_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774774.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="U4A12_002437"
     CDS             190..690
                     /locus_tag="U4A12_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1774775.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="U4A12_002438"
     CDS             787..2634
                     /locus_tag="U4A12_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN1774776.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="U4A12_002439"
     CDS             2637..3533
                     /locus_tag="U4A12_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1774777.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="U4A12_002440"
     CDS             3583..3972
                     /locus_tag="U4A12_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1774778.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6285
                     /locus_tag="U4A12_002441"
     CDS             3959..>6285
                     /locus_tag="U4A12_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1774779.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         2118 a          932 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaa
//
LOCUS       DASJOX010000133         6248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJOX010000133 DASJOX010000000
VERSION     DASJOX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.135"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            232..1500
                     /locus_tag="U4A12_002442"
     CDS             232..1500
                     /locus_tag="U4A12_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEN1774780.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="U4A12_002443"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="U4A12_002443"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN1774781.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="U4A12_002444"
     CDS             3076..3351
                     /locus_tag="U4A12_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN1774782.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="U4A12_002445"
     CDS             3357..4124
                     /locus_tag="U4A12_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN1774783.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="U4A12_002446"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="U4A12_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN1774784.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         2131 a         1113 c         1407 g         1597 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaag
//
LOCUS       DASJOX010000134         6173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJOX010000134 DASJOX010000000
VERSION     DASJOX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.136"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U4A12_002447"
     CDS             725..811
                     /locus_tag="U4A12_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1774785.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U4A12_002448"
     CDS             complement(932..1621)
                     /locus_tag="U4A12_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774786.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U4A12_002449"
     CDS             complement(1628..1906)
                     /locus_tag="U4A12_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774787.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U4A12_002450"
     CDS             complement(1866..2780)
                     /locus_tag="U4A12_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1774788.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U4A12_002451"
     CDS             complement(2762..3142)
                     /locus_tag="U4A12_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1774789.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U4A12_002452"
     CDS             complement(3592..3879)
                     /locus_tag="U4A12_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1774790.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U4A12_002453"
     CDS             complement(3899..4123)
                     /locus_tag="U4A12_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN1774791.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="U4A12_002454"
     CDS             complement(4578..4748)
                     /locus_tag="U4A12_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774792.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U4A12_002455"
     CDS             complement(4763..5296)
                     /locus_tag="U4A12_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1774793.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U4A12_002456"
     CDS             complement(5317..6159)
                     /locus_tag="U4A12_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1774794.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASJOX010000135         6169 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJOX010000135 DASJOX010000000
VERSION     DASJOX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.137"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     regulatory      164..261
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            377..958
                     /locus_tag="U4A12_002457"
     CDS             377..958
                     /locus_tag="U4A12_002457"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN1774795.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            961..2268
                     /locus_tag="U4A12_002458"
     CDS             961..2268
                     /locus_tag="U4A12_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN1774796.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2561..3049
                     /gene="purE"
                     /locus_tag="U4A12_002459"
     CDS             2561..3049
                     /gene="purE"
                     /locus_tag="U4A12_002459"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN1774797.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3042..4166
                     /gene="purK"
                     /locus_tag="U4A12_002460"
     CDS             3042..4166
                     /gene="purK"
                     /locus_tag="U4A12_002460"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN1774798.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4179..5474
                     /gene="purB"
                     /locus_tag="U4A12_002461"
     CDS             4179..5474
                     /gene="purB"
                     /locus_tag="U4A12_002461"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN1774799.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5654..6031)
                     /locus_tag="U4A12_002462"
     CDS             complement(5654..6031)
                     /locus_tag="U4A12_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774800.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT         1932 a         1052 c         1386 g         1799 t
ORIGIN      
        1 gcattaaaaa tcattagatt ttacaaaaaa ataagtaaac cacgttgtca gtttcagaaa
       61 gtagtgttag actgttttca atatttgaaa caacattttt tattcaaata tggcaaactg
      121 ccttcacgtg caaaacagtt gcaagaaaat gaatttacac tattattagt tttacatacc
      181 tacttatata tcgtcataat atggatgaca gtttctagcc agtaccgtaa atgctggact
      241 ataagtaaaa gattggctat ttcacccttc ttttcttatg ataaagtgga gggtgttttt
      301 ctttaacaga aacatagtag gtttttgcac aaaagcggtt atttcggtat taataaatga
      361 taaaggagca aataaagtga aagaattagt tgaacgtatt caaaaagacg gacgtatatt
      421 aggcgagggc gtattaaaag tcgacagctt cgtcactcac caagtagacc ctgtattgat
      481 ggaacaaatg ggaaaacggt ttgcagaagt atttaaagaa caaggaatca cgaaagttgt
      541 gaccattgaa gcttctggga tcgcaccagc tttatttgct gcaagagaat tagatgttcc
      601 aatgatcttc gctagaaaag caaaaagttt gacgatggat gaagaattat tgacagcttc
      661 tgtttattct tttacgaaac aagtgacaag tacgatttct atttctcgta aatttttatc
      721 ttcagaagac aaagtattga ttatcgacga tttcttggca aacggacaag cggcaaaagg
      781 attgatcgaa ttatgccagc aagctggagc gcaagtagaa ggaatcggga tcgtcatcga
      841 aaagtctttc caagatggac gtcagttact tgaagaaatg ggattgagag tcgtttctct
      901 tgcgcgtatt gcttccttga cgaatggaca agttgagttt cttgaggagg acgcataatc
      961 gtggaaaaag aaacacaaaa tggaaaagct gcggttttag ggctgcagca tctgctagcg
     1021 atgtatgcag gagcagtagc ggtgcctctt ttgatcggta caggactagg ctttaatcaa
     1081 gaacagatga cttatttgat ttcaattgat atctttatgt gtggattagc gacattattg
     1141 caattgacag ttaaccgatt tttcggaatt ggcttgcctg tcgttttagg atgtgcgatc
     1201 caagcagtag ctccactgat cttgatcgga accaatgaag gagtaggagc tatctacgga
     1261 tctattatcg cttctgggat ctttgttgtg ttagtttctg gtgtcttttc taaaattaaa
     1321 aaactattcc ctcctatagt aacaggaacg gttatcaccg ttattggttt gactttgatt
     1381 cctgtagcaa tcgagaaaat gggtggaggt aatgcaacag cagctgattt tggtgacaaa
     1441 acaaatctgc tacttgcatt tgtgacgatt ctcctaattg taggcgtgca aatgctggca
     1501 aaagggttca ttcgttcaat cgcggtattg attggtttag tcggaggaag catcttagct
     1561 gctttcatgg gaatgattga tctgagcgcg attggagatg cgccgttgtt ccatgttcca
     1621 caaccctttt attttggtaa accaactttc gacgtatggt cgattttact aatgatcatc
     1681 atttccatgg tcagcatggt agaatcaaca ggtgtatatt ttgcacttgg tgacattact
     1741 gggaaaaatg ttggagaaga agaattgaaa aagggatatc gtgcagaagg acttgccgtt
     1801 atcttaggcg gtattttcaa tacattccct tatactggct tttctcaaaa tgtcggattg
     1861 gttcaacttt cgggaatcaa aacaagaaaa cccatctatt tctctgcatt cttcttgatt
     1921 gttttaggat tattccctaa aatcggtgcg ttagcacaaa tcatccctga gccggtactt
     1981 ggtggaggaa tgttagtcat gttcgggatg gtggcagtcc aaggaatccg tatgctgttg
     2041 gaagttgatt tcagcaatga taaaaatcta ttgattgttg ctgtttctgt tggatttggg
     2101 ctgggtttca atgttacgcc gacattattc agtcaaatgc cgcaaacatt gcaaatgttc
     2161 actggaaatg gcatcgtaat gagtagcatt acggctatca tattgaattt gatctttaat
     2221 ggattgaaaa aagatgaaga aatattggaa gaagccttca agaagtaata gagcaaaaga
     2281 aaggttgtga aatcagcgtt ttgacctgat tgttagagaa acaaacggaa aaatagttcc
     2341 tcgtattttg ccgtttgttt gagctaatac cgagggtcag ctgattgaac accgtttatc
     2401 cataaaaagg aatgtgacct aacttttgtc acattccttt ttttcatctt taataaacac
     2461 gaacaataaa atgtttttct ttgttaacat tcgaaatttc tttgacatca aaaaaagtga
     2521 ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca atgacagcaa aaatctctgt
     2581 gattatgggg agtacttcag attgggaaac aatgaaagaa gcatgtcaaa tattggatga
     2641 attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt
     2701 tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc attattgcag gagcaggggg
     2761 agccgcgcat ctgccgggta tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc
     2821 ggtccaatca cgaacattaa atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg
     2881 aggagtacct gtagctacta cagcaatcgg aaaagcggga gcaattaatg ctggactact
     2941 tgcgatacag atgctttcca tgtatgattt agaattggag gctaaattgg ctgaaagacg
     3001 ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa cttggttaaa cctttgttgc
     3061 ctggttcaac gatcgggatc gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg
     3121 caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac
     3181 aagttgcaga ttggcacatt gaaagtgtct atgatgacac ctttgcgctg aaagagcttg
     3241 ccagaagaac agatgtcatt acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg
     3301 cgattttacc catgtcattt attcctcaag ggacagactt gcttgcgatt acgcaagacc
     3361 gcctgttaga aaaatctttt ttggaaacga ataacatcgt gattgctcct tatgcaacga
     3421 tcgtcagtcc gacggatatc caagatgcga ttgacgggat cggctatcct tgtgttttga
     3481 aaaccactcg aggcggctat gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc
     3541 tagctcctgc catggacctt ttaagagaag gaacctgtgt tttagaagca tggatcccgt
     3601 ttgaaaaaga gatttcgatc atggtggcag gaaatggtca aggagatttc acgacttttc
     3661 cggtcgtgga aaacatccac cacaacaata ttcttcatga aacgattgct ccagccgcga
     3721 ttgatcagga tgtgatcgag gaggctgagc gtattgcccg tgtgattgca gaagcagtga
     3781 gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga
     3841 atgaattagc tcctcgtcct cacaattctg gacattattc tattgaagct tgttctatga
     3901 gtcagttcga tgcccatatt cgaggtattt gtggttggcc attaggagaa aaagtacgtt
     3961 tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca actgctggaa agctatcgct
     4021 tgattgagaa taagccagac tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag
     4081 gtcgcaagat gggacatatc actatcttga cagaagacat ccagcaaacg ctggaagaaa
     4141 tcaaacaaac gaatatttgg gattagagga gaaaaaacat gttagaacgt tatacacgcc
     4201 ctgaaatggg gaatatctgg acggaagaga acaaataccg tgcgtggctc gaagtcgaga
     4261 ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa
     4321 ttcgtgagca tgcatcattt gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc
     4381 atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt gggagaagaa agaaaatggg
     4441 tccattatgg tctaacaagt acggatgtcg tagatactgc ttatggctat ctcatcaagc
     4501 aagcaaatga tattttacgg gaagacttga aacgttttac ggaaatcatt ggcgaaaaag
     4561 caaaagaaca caaatataca gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa
     4621 ctacttttgg attgaaactt gccacatggt attcagaaat gaaacggaac atcgaacgat
     4681 ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg
     4741 caaatatccc gccatttatt gaagagtatg tatgtaaaca attggggatc cgtccacaag
     4801 aaatctctac tcaagttctc cctcgggatc tacatgctga atatttctca gcgatggcgc
     4861 ttattgctac aagtattgaa cgttttgcaa cagagatacg cggattgcaa aaatccgaaa
     4921 cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca
     4981 aaaggaatcc gatcggttca gaaaatatgg ctggtctagc cagagtggtg cgcggacaca
     5041 tggtcactgc ctatgaaaat gtcacattat ggcatgaaag agatatttct cattcttcag
     5101 cagaacgaat catcttgcct gataccacga ttttattgaa ttacatgtta aatcgttttg
     5161 gcaatatcgt taaaaatcta acggtctttc ctgaaaacat gaagcggaat atgtctgcaa
     5221 catttggttt gatttatagc cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa
     5281 gagaagaagc ttatgatctt gttcagccaa aaactgcgta cgcttgggac aaccaagtgc
     5341 agttccgccc gctgttagaa gaagatccaa ccatcacatc ggtattatct aaagaagaaa
     5401 tagacgatgc ctttgattat catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag
     5461 taggtttgga ataaagaaga gtgagttgca aaattcaatt aaaatagaat agcaaaaata
     5521 accaaaaaag aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta
     5581 tcaataatag gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca
     5641 aaaaatagag gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga
     5701 gatgttgcag caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct
     5761 ggatacgata atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc
     5821 ctttgtgttc tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg
     5881 ctcggcggat ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt
     5941 tgatcgacag caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa
     6001 aaagctgacc ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc
     6061 aaatttttag ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg
     6121 tataagattt tcgtttctga taaaacacga acattaaaag aatctaatg
//
LOCUS       DASJOX010000136         6135 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJOX010000136 DASJOX010000000
VERSION     DASJOX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.138"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            102..2543
                     /locus_tag="U4A12_002463"
     CDS             102..2543
                     /locus_tag="U4A12_002463"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN1774801.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="U4A12_002464"
     CDS             complement(2873..3577)
                     /locus_tag="U4A12_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774802.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="U4A12_002465"
     CDS             complement(3764..4009)
                     /locus_tag="U4A12_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774803.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="U4A12_002466"
     CDS             complement(4026..4877)
                     /locus_tag="U4A12_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1774804.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="U4A12_002467"
     CDS             5043..6047
                     /locus_tag="U4A12_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN1774805.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1964 a         1063 c         1297 g         1811 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaa
//
LOCUS       DASJOX010000137         6100 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJOX010000137 DASJOX010000000
VERSION     DASJOX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.139"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(312..797)
                     /locus_tag="U4A12_002468"
     CDS             complement(312..797)
                     /locus_tag="U4A12_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774806.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(936..1151)
                     /locus_tag="U4A12_002469"
     CDS             complement(936..1151)
                     /locus_tag="U4A12_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN1774807.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1375..2175
                     /locus_tag="U4A12_002470"
     CDS             1375..2175
                     /locus_tag="U4A12_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1774808.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2194..4062
                     /locus_tag="U4A12_002471"
     CDS             2194..4062
                     /locus_tag="U4A12_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1774809.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4055..4546
                     /locus_tag="U4A12_002472"
     CDS             4055..4546
                     /locus_tag="U4A12_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN1774810.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4534..5088
                     /gene="srlA"
                     /locus_tag="U4A12_002473"
     CDS             4534..5088
                     /gene="srlA"
                     /locus_tag="U4A12_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN1774811.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5106..>6100
                     /locus_tag="U4A12_002474"
     CDS             5106..>6100
                     /locus_tag="U4A12_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN1774812.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2157 a          968 c         1221 g         1754 t
ORIGIN      
        1 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
       61 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
      121 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
      181 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
      241 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
      301 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
      361 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
      421 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
      481 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
      541 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
      601 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
      661 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
      721 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
      781 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
      841 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
      901 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
      961 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     1021 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     1081 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     1141 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     1201 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     1261 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     1321 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     1381 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     1441 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     1501 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     1561 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     1621 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     1681 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     1741 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     1801 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     1861 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     1921 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     1981 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     2041 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     2101 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     2161 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     2221 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     2281 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     2341 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     2401 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     2461 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     2521 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     2581 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     2641 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     2701 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     2761 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     2821 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     2881 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     2941 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     3001 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     3061 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     3121 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     3181 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     3241 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     3301 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     3361 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     3421 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     3481 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     3541 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     3601 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     3661 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     3721 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     3781 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     3841 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     3901 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     3961 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     4021 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     4081 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     4141 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     4201 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     4261 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     4321 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     4381 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     4441 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     4501 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     4561 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     4621 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     4681 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     4741 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     4801 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     4861 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     4921 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     4981 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     5041 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     5101 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     5161 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     5221 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     5281 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     5341 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     5401 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     5461 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     5521 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     5581 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     5641 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     5701 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     5761 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     5821 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     5881 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     5941 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     6001 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     6061 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DASJOX010000138         5918 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJOX010000138 DASJOX010000000
VERSION     DASJOX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.140"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1055)
                     /locus_tag="U4A12_002475"
     CDS             complement(552..1055)
                     /locus_tag="U4A12_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1774813.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1414..2439
                     /locus_tag="U4A12_002476"
     CDS             1414..2439
                     /locus_tag="U4A12_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1774814.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2762..3640
                     /locus_tag="U4A12_002477"
     CDS             2762..3640
                     /locus_tag="U4A12_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN1774815.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4116..4232
                     /locus_tag="U4A12_002478"
     CDS             4116..4232
                     /locus_tag="U4A12_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1774816.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5160..5498
                     /locus_tag="U4A12_002479"
     CDS             5160..5498
                     /locus_tag="U4A12_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774817.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5592..5822
                     /locus_tag="U4A12_002480"
     CDS             5592..5822
                     /locus_tag="U4A12_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774818.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2014 a          887 c         1086 g         1931 t
ORIGIN      
        1 aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct tttgttattt
       61 tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta ctacttctaa
      121 aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca gaatatactg
      181 gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat ataaagtaat
      241 tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga
      301 cggaattcct ttctagattg agatattgac taggaagaat gttaagattt taagccttta
      361 agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc
      421 aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa
      481 tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt
      541 tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga
      601 aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga
      661 tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc
      721 aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag
      781 cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc
      841 aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc
      901 gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg
      961 agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa
     1021 taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta
     1081 taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt
     1141 atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag
     1201 atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg
     1261 ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa
     1321 gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag
     1381 ctttttttcc aattactagg aaggagggat agattgttca agacttattt tcgtttcaat
     1441 aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta
     1501 tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt
     1561 acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc
     1621 ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt
     1681 gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat
     1741 atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag
     1801 ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc
     1861 ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa
     1921 gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac
     1981 agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat
     2041 tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat
     2101 cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca
     2161 tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca
     2221 caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc gatggcgttg
     2281 atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta
     2341 gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg
     2401 caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa
     2461 aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt
     2521 ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata
     2581 tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc tgagatggtc
     2641 aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta agttttatag
     2701 ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag tcatttaaac
     2761 aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca atgaaatcca
     2821 aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga ttgtacaagc
     2881 aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg atcctaataa
     2941 agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt ttgcagcatt
     3001 agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac cattgaagat
     3061 tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc atgaacattt
     3121 ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt acactagtgg
     3181 cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga aagggtacac
     3241 ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg atgcagattt
     3301 atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg aactttattt
     3361 tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac ccaatcttac
     3421 taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc gattattatc
     3481 agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag atgcccaaat
     3541 acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat tgcttgcagg
     3601 cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact tcgaaatagt
     3661 ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     3721 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     3781 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     3841 ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa caatagatat
     3901 taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat gaaaatttat
     3961 ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt tgactttcat
     4021 tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc
     4081 atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat atctcaagtg
     4141 cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg aagatcatca
     4201 aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag
     4261 ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt tccgaaagaa
     4321 ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc ttttcaaatt
     4381 gataccattc cctaattcca gttatatagt taactacaga accacaaagc acgtacgttt
     4441 ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt ttgaccagta
     4501 gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt tagtcaagaa
     4561 tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata tttcatgtag
     4621 aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac gaaagctccc
     4681 aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag tgattcgtat
     4741 ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa
     4801 aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt ttggttttca
     4861 gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt atttttcatg
     4921 aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt aaggaggaac
     4981 ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag actcaaagtt
     5041 gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga agggcttttt
     5101 tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta acattttcta
     5161 tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca gaagagttta
     5221 agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca attactgata
     5281 ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta aataatgact
     5341 caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg agttcattgt
     5401 taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg gattactcaa
     5461 aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa tactttatga
     5521 tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc aaaagcagga
     5581 agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa
     5641 aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca attaactcga
     5701 ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt tggactaaag
     5761 aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt atgcaacagt
     5821 aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt ttcgatatct
     5881 ttgctgttaa tattactaac tttaatgcag tataaaga
//
LOCUS       DASJOX010000139         5886 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJOX010000139 DASJOX010000000
VERSION     DASJOX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.141"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..1170)
                     /locus_tag="U4A12_002481"
     CDS             complement(76..1170)
                     /locus_tag="U4A12_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1774819.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="U4A12_002482"
     CDS             1359..2120
                     /locus_tag="U4A12_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1774820.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="U4A12_002483"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="U4A12_002483"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1774821.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="U4A12_002484"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="U4A12_002484"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN1774822.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="U4A12_002485"
     CDS             complement(3760..4773)
                     /locus_tag="U4A12_002485"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN1774823.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="U4A12_002486"
     CDS             4938..5858
                     /locus_tag="U4A12_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN1774824.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1789 a         1176 c         1054 g         1867 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcac
//
LOCUS       DASJOX010000140         5850 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJOX010000140 DASJOX010000000
VERSION     DASJOX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.142"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(92..1390)
                     /gene="eno"
                     /locus_tag="U4A12_002487"
     CDS             complement(92..1390)
                     /gene="eno"
                     /locus_tag="U4A12_002487"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN1774825.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1506..2261)
                     /locus_tag="U4A12_002488"
     CDS             complement(1506..2261)
                     /locus_tag="U4A12_002488"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN1774826.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2365..3555)
                     /locus_tag="U4A12_002489"
     CDS             complement(2365..3555)
                     /locus_tag="U4A12_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN1774827.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3665..4666)
                     /gene="gap"
                     /locus_tag="U4A12_002490"
     CDS             complement(3665..4666)
                     /gene="gap"
                     /locus_tag="U4A12_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1774828.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4707..5798)
                     /locus_tag="U4A12_002491"
     CDS             complement(4707..5798)
                     /locus_tag="U4A12_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN1774829.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
BASE COUNT         1518 a         1236 c         1096 g         2000 t
ORIGIN      
        1 atttcagcaa gtagagagtt cgattactta actaagtgat catactcgac tatctttcaa
       61 tagtcgatca tgtatcttca ttagaaaaga attatttgtt ttttaagttg tagaaagatt
      121 tcaaaccttt gtattctgca acttcaccaa gttggtcttc gatacgtagt aattggttgt
      181 atttagcaat acggtcagta cgtgatagag aaccagtttt gatttggcca gcgtttgttg
      241 caacagcgat atcagagatt gttgaatctt ctgtttcacc agaacggtga gatacaactg
      301 ctgtgtagcc agcttcttta gccatttcga tagcttcgaa agtttctgtt aatgtaccga
      361 tttggttcac tttgataagg attgagttag caattccttt ttcgatacct tcagataatt
      421 tttgagtgtt tgttacgaac aagtcgtcac caactaattg aactttgtcg cctaatacgt
      481 cagttaattt cttgaatccg tcccagtcgt tttcgtctaa tccgtcttcg attgagatga
      541 ttggatattt agaaactaat tcttcgtaga atttgatcat ttcgtcagtt gttttttcgc
      601 cttcgcctga atcagctagt acgtaaacac ctttttcttt gtcgtagaat tctgaagaag
      661 cagcatccat agcaagaaca acgtctttac caggtacata gccagctttt tcgattgctt
      721 cgatgattac ttcaaaacct tcttcgtttg aaccaaggtt tggagcaaat ccaccttcgt
      781 caccaacaga agttgctaat ccgcgagctt tcaagattga agctaatgcg tggaatactt
      841 cagcacccat acgtagagct tctttgaatg ttggagcgcc aacaggcatg atcatgaatt
      901 cttggaagtc gattgagtta tcagcatgtg atccgccgtt gatgatgttc atcattggtg
      961 ttggcaatac ttttgtgttg aatccgccta ggtagtggta taaaggtact tctaggtagt
     1021 cagcagctgc acgtgcaaca gcgatagaaa cacctaaaat agcgttcgca cctaatttac
     1081 ctttgttagg agttccgtct aaagcgatca tagctttgtc gatagccatt tggtcacgaa
     1141 catcgtagcc aatgattgct tcagcaatga tgttgtttac gttgtcgaca gctttagtta
     1201 cacctttacc gccataacga gctttgtctc cgtcacgtaa ttcaactgct tcgtattcac
     1261 cagttgaagc acctgaagga accattccgc ggccaaaagc tccgctttct gtgtatactt
     1321 ctacttcgat tgttgggtta ccacgtgagt ctaagatctc gcgagcgtaa acatcagtaa
     1381 taattgacat gttttgtctc tcctttgagt ttgttttttc ctaagggatt tccctcatat
     1441 acgattttag ttaattttgc tcattagtgc aaacatttct tgcatttttc gcaaattttc
     1501 aacaattatt ttacagcgtc taacaatgac aagaatgaat ctgcttgcaa gcttgcgcca
     1561 ccgactaaag caccgtcaac attttctttt gccatgtatt cagcaatatt ttctggtttt
     1621 acagaaccgc cgtattggat acgaactgct tctgatactt cttttccata aagtttttca
     1681 actgtttgac gtacaactcc acaaatttcg tcagcgattt gtgcgtcagc tgatttacca
     1741 gtaccgattg cccagattgg ttcataagca ataaccatgt ttgacacttg ttcagctgaa
     1801 agatcagcta aaccagctgt aatttgtcct tcgatccatt cagcagtttt gcctgcttca
     1861 taggtttcta aagattcgcc acagcatagg attggtgtca taccatttgc aaagatagct
     1921 ttcgcttttt tattgatgtc ttcgtctgtt tcatggaaat attcacgacg ttctgaatgt
     1981 ccgatgatca cataatgaac acctagatca gcaagagctg ctggtgaagt ttcaccagtg
     2041 aacgcacctg agttttccca gtagcagttt tgtgctgaga tttttagttc tgtaccttca
     2101 gctgatgtca ttaattcttg taagaataat gcaggagatc cgatgactga atcaactttg
     2161 tcatttgaag ggattttgtt ttttactgct tcagcaaatt cttttgcttc tgatgcagtt
     2221 ttgttcattt tccagttacc tgcgataata ggtttacgca ttgaggaaca cactccttaa
     2281 tttattgaaa aatggctagg gagtcatgaa aactcccttc accttttgat cgattactcg
     2341 tcagaattga gcaaacttgg tcgtttattt atcgttgatt gctgctagtc ctggtaattc
     2401 tttaccttca agcaattcca agcttgcgcc gccacctgtt gagatgtgtg tgaatttgtc
     2461 agcaaatcct aattgttctg ctgctgcggc tgagtctccg ccaccgatga ttgttgttgc
     2521 gccttctaag ttagcgattg cttcacaaac accgattgta cctttagcaa agttactcat
     2581 ttcaaataca cccataggtc cgttccaaac aactgttttt gcaccttgta atgtatcagc
     2641 aaataatttt actgatgctg ggccgatgtc taaagccatc aaaccatcag gaatgtctcc
     2701 ttcgattact tgtgtttcaa catcatttga aaattctggt gcacagacat tgtcgattgg
     2761 caagacaagt ttgtcgcctg ctttttcgat taattctttt gctagttcca ctttatcagc
     2821 ttctactaga gagttaccga tcttgattcc ttttgcttcg tagaatgtgt aagtcattcc
     2881 gccaccgatc aagattttat ctgctttagg aatcaagttt tcgattacgc cgattttatc
     2941 agaaactttt gcgccaccaa ggattgcaat cattggtcgt tttggttctt ctactgcttc
     3001 accgatgaac ttgatttctt tttccatcaa gaatccagca actgttggga taccagttga
     3061 agcgatacct acgttagaag catgtgcacg gtgagctgta ccgaaagcat cgttaacaaa
     3121 tacatcgcct aatgaagccc agtatttacc tagttctgta tcatttttgc tttctttttt
     3181 accgtcaata tcttcaaatc gtgtattttc gaatacgaca acgtcgccgt cattcatgtt
     3241 gcggattgct tcttctaatt gttctccgcg agtttcaggt acgaatgtta cttctttacc
     3301 taataattcg cctagacgtt ttgcaactgg agctaaagat tttccttctt tatcttcttc
     3361 tgtttttaca cgtccaaggt gagagaaaag gattgctttt ccgccttgtt ctagaacgta
     3421 gttgattgtt ggcaatgctg ctttgatacg agtgtcgtca gtgatcacgc cgtctttcaa
     3481 aggaacgttg aagtcaacac ggacaagaac ttttttgtct tttaaatcga tatctttcac
     3541 tgttttctta gccattttat ttcctcctga ataaacttat tatcaaaaaa agcggaagaa
     3601 gcgcgattgc ttccccgctt ttaaatgtac atgtctataa tttacacttg tacgtttgtc
     3661 tatcttataa gttagcgaag tattctaaag tacgtactaa ttgtgcagtg tatgacattt
     3721 cgttgtcgta ccaagcaaca gttttaacta attgtttgtc gccaactgtc attactttag
     3781 tttgtgtagc atcaaataat gaaccgtaag tcataccaac gatatctgaa gaaacgattt
     3841 cgtctgtgtt gtatccgtaa gattcgtttg aagcttcttt cattgctgca ttgatttcgt
     3901 caacagttac ttctttttca agaactgtta ctaattctgt taatgaacct gttggtacag
     3961 gaacacgttg agcagctccg tctaatttac cgttcaattc aggaattact aaaccgatag
     4021 ctttagcagc acctgttgag ttaggaacga tgtttgcagc agcagcgcgt gcacggcgga
     4081 agtcaccttt aggatgaggt ccgtctagag tcatttggtc acctgtgtaa gcgtggatag
     4141 ttgtcattaa tccttcaaca acaccaaatt tgtcgttcaa tgttttagcc ataggagcta
     4201 aacagttagt agtacatgaa gcacctgaga taactgtttc tttaccagtt aatgtttcgt
     4261 ggtttgtgtt ataaacgatt gttggaacat cgtttccgcc aggagcagag ataacaacgc
     4321 gtttagcacc agctgttaaa tgtttttcag ctgctgattt agaagtgaag aaaccagtac
     4381 attctagaac gatatctacg cctagttcgc cccatggtaa ttcttctggg ttgcggttag
     4441 ctaatacttt aacttctttt ccgtttacgt tgaatgaacc ttcatgaact tcaactgttc
     4501 cgttgaaacg tccttgagtt gtgtcgtatt ttaacaagtg tgccaacatt ttagcatctg
     4561 tcaagtcgtt gattgctact acttcgattc catctacatc ttggatacga cggaatgcta
     4621 gacgtccaat acgtccaaaa ccattaatac ctactttaac tgtcatttaa gatttcctcc
     4681 ttatgaaaat caaaaaaata tttattttaa agggttaccc cttttaaaat ctcatttgca
     4741 gcagcttcat cagtgatgag ccacgtttgt tttggtgcat ttttcatata agctcgaatt
     4801 gctttggctt ttgattttcc gcccgcgatt gccatgatga tcgggacttt ttccaaatct
     4861 tgcaattcta tgccgattct agggacctta taaacgactt cacctttttc attgaaaaag
     4921 tagccaaaag attccgcaac agcatttgct tgtttcagca tgactaattc tttttcagtc
     4981 atcttacgac gtgctgccat gtgtaatgca cgcccaatac tatgaataac acaattcgct
     5041 tcactaatca agtttaagac ttcttggata gatggctcat tgagtaaaga attgtaagtt
     5101 gccagactta attgttccgg aacataaagt gcacggtggt taccgtttgc tttagcagcc
     5161 attcttgcac tgacagaatt tgcctgtaca gttatcgctt caccaatccc gcctcttgca
     5221 gggacaaaca ggttatgccg tttcttattt tccagattag acaattgttc ggctaccaca
     5281 gccatagtag ttcctcccat cacagcgatg atattctcgc cttctggaag tagtcggtct
     5341 agggcttcgc tcagcgcttc gccgaatgca tcagccactt tggtctgctc ttcgctgttt
     5401 cctgaaacaa cgatacagcg ttggttacca aaatattctg ccaactgttg ttcggtttga
     5461 tgcgtaccta aaagctgatc catgaagttt tcaaggcttt gataaacttc ttcgccctta
     5521 gttgtcagcg tcataccgct ttttgaggtg tcgatcaaat tcagattttt caaaagatct
     5581 gtttctgttc gcaatactcg ttctgtgatc cccatacttt ccgataaact tcgacgtcca
     5641 atcggctgca tccaatagat atttcgtaaa attttatagc gttcttgcaa cacttcgata
     5701 atatctggtg caacagattc aatcattttt atttcatcca gcatagcgat ctccttagtt
     5761 ggacacttta cgtccaatgt agacttaaaa cgacccatat acgataaaaa aagaaattaa
     5821 ctattcgaat ttcacatcaa taaagggaaa
//
LOCUS       DASJOX010000141         5790 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJOX010000141 DASJOX010000000
VERSION     DASJOX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.144"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4A12_002492"
     CDS             complement(99..1229)
                     /locus_tag="U4A12_002492"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN1774830.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="U4A12_002493"
     CDS             complement(1877..3079)
                     /locus_tag="U4A12_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1774831.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="U4A12_002494"
     CDS             complement(3139..3342)
                     /locus_tag="U4A12_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN1774832.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="U4A12_002495"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="U4A12_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="U4A12_002496"
     CDS             4879..5691
                     /locus_tag="U4A12_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774833.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DASJOX010000142         5788 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJOX010000142 DASJOX010000000
VERSION     DASJOX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.145"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..2197)
                     /gene="copB"
                     /locus_tag="U4A12_002497"
     CDS             complement(26..2197)
                     /gene="copB"
                     /locus_tag="U4A12_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN1774834.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2217..4403)
                     /locus_tag="U4A12_002498"
     CDS             complement(2217..4403)
                     /locus_tag="U4A12_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1774835.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="U4A12_002499"
     CDS             complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="U4A12_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN1774836.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4625..5065)
                     /locus_tag="U4A12_002500"
     CDS             complement(4625..5065)
                     /locus_tag="U4A12_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN1774837.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5139..5678)
                     /locus_tag="U4A12_002501"
     CDS             complement(5139..5678)
                     /locus_tag="U4A12_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1774838.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1496 a         1302 c         1009 g         1981 t
ORIGIN      
        1 tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta
       61 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
      121 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
      181 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
      241 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
      301 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
      361 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
      421 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
      481 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
      541 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
      601 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
      661 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
      721 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
      781 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
      841 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
      901 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
      961 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     1021 tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag
     1081 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     1141 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     1201 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     1261 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     1321 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     1381 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     1441 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     1501 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     1561 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     1621 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     1681 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     1741 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     1801 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     1861 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     1921 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     1981 aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     2041 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     2101 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     2161 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     2221 tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc
     2281 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     2341 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     2401 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     2461 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     2521 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     2581 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     2641 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     2701 tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca
     2761 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     2821 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     2881 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     2941 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     3001 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     3061 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     3121 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     3181 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
     3241 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     3301 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     3361 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     3421 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta
     3481 acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga
     3541 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     3601 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     3661 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     3721 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     3781 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     3841 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     3901 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     3961 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     4021 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     4081 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     4141 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     4201 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     4261 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     4321 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     4381 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     4441 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     4501 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     4561 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     4621 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     4681 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     4741 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     4801 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga
     4861 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
     4921 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     4981 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     5041 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     5101 atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
     5161 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     5221 cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc
     5281 cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc
     5341 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     5401 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     5461 tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat
     5521 tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa
     5581 aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat
     5641 tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg
     5701 gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag
     5761 tcgtgtcaaa gtcccttgtt tcgaataa
//
LOCUS       DASJOX010000143         5787 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJOX010000143 DASJOX010000000
VERSION     DASJOX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.146"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..450
                     /locus_tag="U4A12_002502"
     CDS             34..450
                     /locus_tag="U4A12_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774839.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
     gene            1107..2261
                     /locus_tag="U4A12_002503"
     CDS             1107..2261
                     /locus_tag="U4A12_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN1774840.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2255..2677
                     /locus_tag="U4A12_002504"
     CDS             2255..2677
                     /locus_tag="U4A12_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774841.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3800..4732
                     /locus_tag="U4A12_002505"
     CDS             3800..4732
                     /locus_tag="U4A12_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1774842.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4749..5264
                     /locus_tag="U4A12_002506"
     CDS             4749..5264
                     /locus_tag="U4A12_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1774843.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            5560..>5787
                     /locus_tag="U4A12_002507"
     CDS             5560..>5787
                     /locus_tag="U4A12_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774844.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLN"
BASE COUNT         2219 a          955 c          928 g         1685 t
ORIGIN      
        1 aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac
       61 gttacccttt atcccagtca cattgacgtt ttagattcca tcatcgaaaa aaatccagat
      121 atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc tgataccaat
      181 acaacaacta ccagtaacca agccaaacta aatgcgatgg gaaaagaaat ttctatcatg
      241 accgaaatta tcgcagaatt tgctgatttg tatttaaaag atacaggcgt tcttatcggt
      301 acaaactcga aagtatatca agaagtaaaa gaaaaaattg aaaaacagct aaatccctcc
      361 caacgaaatc aaccaaagaa tcgtcaaaaa gaaacgagtc aaacattcac ttctaaaaat
      421 ttcgacccaa ccaactttct aaaaagataa aaaaataaac caggaaattt ttaatttcct
      481 ggtttatttt ctagagagcg gtcttccaat aagatttgca aatcgatccc aatttgcgta
      541 aaaaaaaagc tgctcgtacg agcagctaaa caagaaaaag tcagcttgtt ttaagggtaa
      601 aataagtgta acaaattttg cgaatgcaac gatctttttt attatacgca aacaatcaaa
      661 aaaaaagact gctcgaaaga gcagtctcaa gcaaaaaata aacgaacaaa cgcaattact
      721 cacgaacaca aacaaataca ataaaatacc taaataatga aaataagtga taaaaaacaa
      781 gggtatttat agtttaacaa gcgctcataa aaataacaag tctttaaaaa atgacagata
      841 aaaatgcaca aagaatcgga cacagataac cgcgaaaaat caagctttag aaagttgttt
      901 ttagctagac caatgaaaaa tcacaaagtc cgcccctgta tccgttggta gaggggctac
      961 ttggtttagt atacattatg ttaactagct cgttaaataa cgattatgtt cccaaaacaa
     1021 tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt
     1081 atatcactta attttaacac aaaggcgtga ttattttgaa acccaaaaat aaaatttatc
     1141 taggtctcct cttttccttg atgattggaa tttttagtat aggaattaca aatacctatg
     1201 cttcagaaaa ttctaatttc aatatcaact cccaaccaat tgaagaacaa gcaattaatt
     1261 ttgcgaaaac tgattttgaa cactccataa cttcaattct tttaatggaa aataacactg
     1321 aaactgatat taactatact cttggttcgc catttaaaat gctcgatgga gaaaatacgg
     1381 caaatagttc tattttcttt ccagtatttt cagacgctga taatacgatt aaatatgttt
     1441 ataccgtcaa aaaaaataca aatggagaat attcaggaag tatttcacaa tttttagcaa
     1501 aagaacttca aaatctagta gatagcaata taacagatac aaccttattt tttcaagata
     1561 atagcattta ttattccgat aatggccaaa ttaaactact ttggaaatca cctttaagta
     1621 cagaaaaaaa tggtgtctct tcctttacag aaaaaaatga tcttatatcc gtaaatataa
     1681 aaaataaaat taaaaatttc tctaaaaaac aaaattttcc tgcggttaaa cgtgtaaaaa
     1741 gtgtctcctc cggagataac tttattatga ttaattggcg tatagcagaa acccaaacaa
     1801 gttttccgtg gtgcgctgct tacgtagaag ctgctatctt atgtaataaa acagatgtac
     1861 gcgctacttc agctagagcc attatgcatt atacctatcc taatttaaac aacagtcaaa
     1921 ttgaatcaaa aagtcttaat caagatcaaa taataaaata tgcaaattac tgtggtgttt
     1981 atcctaaaaa agtagcacga atactaaacc tttcagaggt ccaaacacaa ctaagaaaag
     2041 gaaatgccat ttatatggga actgctggtt atggaaatta tagtaacagc cgtcatgcac
     2101 ttgcattatt tgggtgggtc aatcaaggtg gttatcaaac ttactatgtg tggaatcctt
     2161 ggtataacta cccaactgta ataagtgcaa atgcagggcc agttacaatt cctgtacctg
     2221 gaggtggttt tacttgggac aataccatta caaattggta aataaaccca ttgttttagt
     2281 tatgggtcta gtgttagttc ttttcttaac tagttgttca aaaaaacaag aaatacaaaa
     2341 gactgagctg attccttttc taggaacttg gcaaactaaa gatcactcag aacgtctagt
     2401 aatcactaat ttaaatactt caaaaaatat agaacaatat accatcttag taaaagagaa
     2461 caatcataaa attcaaatca ttgataaaaa tttaaatata aatgaacaag ttttcattta
     2521 caaagcaaca aataatttac taacactgcg ctctaataaa aaacaattag attacacttt
     2581 tagtttggat tctccaaaaa agctcgtatt tattttgaca acgaatgatg ggacagaagg
     2641 gacagctaaa ccaattgaat tttttaaagc tgattgatga atagttgttt gatattacaa
     2701 aacaataatt tcaccgaagg tgaaacaagc gaaactttac aaaaagtggc tagcatttgc
     2761 tagccttttt tgtattgcgg agcgtgagtt ttgaggggga ttcaaggggg agtttctccc
     2821 ctttgacaag cggttatttg caccaaatac gaaggtattt ggctagcaaa tatagagctt
     2881 gccaaaccct ttagtgtgtt ttgtagaaag ggaccttata gtcactttca cattacaaac
     2941 gatacgtcct atggacgtat caaaagggtg ggacttatga gcaaattaaa gcaaaatttg
     3001 gctagcaatc cttctaaaaa atacacctat tgatcagagt caaaataaat cagttttcga
     3061 gtgagcgaat ccgaatttgc aaatctgaag ctttgcaaat gagtgtgccg gcttccgtaa
     3121 aagtctaggt aacattagtc gatatataca taaggctcta atttgcgttt taaggcgttt
     3181 taaacgttct ggtatgtaat ttataccaaa gcgactttaa aacgccttaa aacgaatttt
     3241 ataggtcttg taggacgttt cagggttgtt agggcttgcc ctgaccgcct gtaaggcgct
     3301 ttaaaaacaa gataagggat tttatctaga cttgtcttaa aacgccgtag atgagcgctc
     3361 agagcttctt aaaggactcc ttacgaactg tttttaagca aaggagcctt taggcgactg
     3421 cgcagttcaa tgtgagagcg tagcgatcac acctttaaag cgaccgtagg gagcagattc
     3481 ccagtttagg aaatttcgcg aacctgtttg gagaaaaaaa gtaggaaatt tttaggcggg
     3541 aaattttgcc cccctctttt ataatctctt ttataacctc tttttaaacc tctttaccgt
     3601 cggctgtatc cttactctcc caaggcctgt agctttttta gggcatgaaa ttatctgttt
     3661 ataggcatga aattatctgt ttataggcat gaaattatct gtttataggc atgaaattat
     3721 ctgttttagg tattgtttta agacataata taccttaaaa ttatgtcaat aaaattagtt
     3781 agactggagt atcaaataaa tggcaaatga aatcgtaaaa tataaaccag aattaaatac
     3841 cattccttta aggaaattta ctccaataga aatgaacttg tttttttcta ttgtttctag
     3901 aatgcgtgat aaaggtaatc aaaaaatacg cttttatttc gatcagctta aagatttaag
     3961 tgcttataag caaactgctg gagaagtctt tataaaagat ttaacaagaa catacgatca
     4021 tttaatggat ttacgttttg gaaaaaaaag caatggtgga ttaaccatag aacgatttac
     4081 aatgtttact gattttgaaa ttcatggaga agccgataac ccttatgtgg atataaaagt
     4141 tcacgaaaaa gctttgcctt tacttaacaa tttagaaagt tgggtacgtt tctctttaac
     4201 agaatttcga gatttaaata gcagttatgc taaaacaatg tttagattac taaaacaata
     4261 ccgaacacaa ggatgggctg aattttccaa ggaagatttt ttagaactat tagatattcc
     4321 taaaagctat agacaaacaa atataaatca gtttgtactc aaacccataa aagaagaact
     4381 cacaccatta tttaaaggat taacgattcg taaaaaatat ggtaaaggcc gaggcaaacc
     4441 tgttattggt tatcgtttta cttggaaagc tgaaattaat catgctgacg atttttcaaa
     4501 agggaagcaa gaagatttac gtacaaaatt atttaatatt gaacacaatg gagaattaac
     4561 gcaagaagaa aaatggcgcg ctaaagatcg aatactagat cttccgttag gtactcatga
     4621 agctaatttt aaaaaacaac aacaaactga aaaagaagaa gtggaaaaac aaacactttc
     4681 aaacgaactt aaacaagatt tattaggaaa tttacaaaac ttattcgatt aaagtataag
     4741 gagtatttat ggcagataaa acgatcaaag accttgctga agaattagga gttagtaaga
     4801 cagctattaa caaaaaagtt tctgacgaaa atcgaaaact ttggtttgcg aaaatcggaa
     4861 acaagtttgt gattaacgaa atcggtcaaa aagctataaa atcaatgttt tttaccgaaa
     4921 acgaaaactc aaatcaacaa ccagttggcg aaaaatcgaa aaccgaaaac cataaaaacg
     4981 ccaaccaagt ttccgaatta gaactctttt ttttcctaga aaaacaatta aatcaaaaag
     5041 atatacagct acaagaaaaa gacgaacaat taaaacaaat gcaaaaatta ctcgaccagc
     5101 aacaacaact caccttacaa gccaatcaac aaatcaaaaa acttcacgaa caactccagc
     5161 taacctatgc agaagaatct tcagaaaaag aacccctttc tgttactgaa gaaactcaaa
     5221 aaggacaaaa caaggagaaa aaatggtggc aattctggca ataacccaac aatttttatt
     5281 cataaaaatt gttgggttac tttctttttt tgcttttaca agcatagcga catgatctca
     5341 cgtcgctatt ttttaaagag cataaggaaa cgcggtatca ctttcttacg aaaggtgcta
     5401 ggtaggctgt gcctaccacg tttctaagaa aaaaatttgc tatcactttt tcgccattgt
     5461 ggctagctta tgtatagtat ttcggtaaaa aacagctata caaatactat atttgtatga
     5521 tatacataaa ttatacacac ataagaaagg acgttttcta tggacatttt agttcgaaat
     5581 atcaatccaa aaattattac aagagtagat aaacgttgtc aggaacttac aaccaaaaca
     5641 ggagaaaaat ggagtcgaaa taaatattta aaagtgctaa tcgaaagtga ttttgaccgt
     5701 caacttatga attacaaaaa agagcaattc gatttattac tcgaaaaatt tatcgatatt
     5761 caaacaaaaa atactgaaac attgaac
//
LOCUS       DASJOX010000144         5731 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJOX010000144 DASJOX010000000
VERSION     DASJOX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.147"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            362..1873
                     /gene="gadC"
                     /locus_tag="U4A12_002508"
     CDS             362..1873
                     /gene="gadC"
                     /locus_tag="U4A12_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEN1774845.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1886..3286
                     /locus_tag="U4A12_002509"
     CDS             1886..3286
                     /locus_tag="U4A12_002509"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEN1774846.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3451..4891)
                     /locus_tag="U4A12_002510"
                     /pseudo
     CDS             complement(3451..4891)
                     /locus_tag="U4A12_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5284..5731
                     /locus_tag="U4A12_002511"
                     /pseudo
     CDS             5284..5731
                     /locus_tag="U4A12_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1836 a          919 c         1089 g         1887 t
ORIGIN      
        1 acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac
       61 cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga
      121 atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt
      181 tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga
      241 attttgcttg ctatactttg tcttgtagaa aagaggtgga caaaataatg caagctcaaa
      301 taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa
      361 tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt
      421 gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt tctttcttct
      481 tttgggaggt attttatggt ttctaccagt tgctttatgt gctgcagaaa tggcaacagt
      541 tgaaggatgg aaagatggtg ggattttcag ttgggttagt caaactttag gagaacgttt
      601 tggttttgca gctatttttt tccaatggtt tcaaattaca gtaggtttta ttacaatgat
      661 ctacttcata ctaggagcgc tgtcgtatgt aattaatttt ccagagctta atacagatcc
      721 atttatcaag tttattggtg tattagtcat tttctgggta ctgacttttt cacagttggg
      781 cgggaccaat cgtaccgcta aaattgctaa agcaggattt gtcattggaa ttgttattcc
      841 ttcgatcatt ctctttggtt tagcagcagc ctactttatc ggcggtaatc cgattcaaat
      901 ggctattaat gcgaaagctt ttattcctga cttttctcag ctttcaactt tagtagtatt
      961 tgtatctttt atattggctt atatgggcgt agaagcttca gcatcacata ttaatgagct
     1021 ggaaaatccc caaagaaact acccattggc aatgttttta ctagtgattt tggccatcgc
     1081 cttagatgca atcggcggtt tctcagtagc agcggttatt ccacaacatg acttgtctct
     1141 cagtgctggt gtaatccaaa catttgaaac gcttatttta cattttaaca gtcatttaca
     1201 ttggttggta aaagtgattg ctctcatgat tgcctgtggc gttatgggtg aagttagttc
     1261 ttgggtagtt ggtccatcac gagggatgtt tgcagctgct cagcgaggtc tgttaccagt
     1321 tttcttccgg aaaactaata aacaagacgt tccagttccg ttgattatga tacaaggaat
     1381 tatcgttact atttgggcag cagtcttaac ttttggtgga ggaggcaaca atttatcatt
     1441 cttagtagca atctccttaa ctgttgtcat ttatctcgta ggatatttac tatttttcct
     1501 aggttacttt gtattagttt ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg
     1561 aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt attttatcag tgttcgcatt
     1621 gcttatctct tttgtaccac cagcttcaat tgcagctagt gagcaccgaa cataccaaat
     1681 catactactt attagctttg tagtgactgt tcttttacct tttatcattt atgaagtaca
     1741 tgataaaagt gctcacacaa ctattgagga acctactcac gtgaaagctg aagacgttaa
     1801 tcccgctgtt tatccagctg ctagaggaga gcaccacgtt acaaaaaatg atgaacatat
     1861 tttaaaacat taggaggaat tcattatgtt atacggaaaa gataatcaag aagaaaaaaa
     1921 ctatttggaa ccaatttttg gctctgcaag tgaggatgtt gacttgccta aatataagtt
     1981 aaacaaagaa tccattgaac cacgaattgc ttatcaatta gtacaagacg agatgttgga
     2041 tgaaggaaat gcgcgattaa acttagctac tttttgtcaa acgtatatgg aacctgaagc
     2101 agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt gataaatcag aatacccacg
     2161 aacaacggaa attgaaaacc gctgtgtaaa tatgattgct gatttatggc atgctccaaa
     2221 taatgaaaaa ttcatgggaa cttcaacgat cggctcttca gaagcctgca tgctgggtgg
     2281 tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc
     2341 aaaaaaacct aacctggtga tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt
     2401 atattgggat gtggaactga gagaagtccc aatggatgaa aaacatatgt caattaatct
     2461 agatactgtc atggattatg tggatgagta cacaattggt attgtaggta ttatgggtat
     2521 tacttacact ggtcgttatg atgatatcaa gggtctgaat gatttagttg aagctcacaa
     2581 taaacaaact gactataaag tatacattca tgttgacgct gcatcgggtg gcttttatgc
     2641 accatttact gaacctgatc tagtttggga ttttcaattg aaaaatgtta tctcaattaa
     2701 ttcttcaggt cacaaatatg gtttggtata tccaggtgtg ggttgggtct tatggcgtga
     2761 ccaacaatac ttaccagaag aattagtatt taaagttagt tacttaggtg gagaaatgcc
     2821 aactatggct atcaacttct ctcatagtgc agcacaacta attgggcaat actacaactt
     2881 tgtacgctat ggctttgatg gttatcgtga tattcaccaa agaactcatg atgttgctgt
     2941 ttatttagcc aaagagatcg aaaaaactgg tatttttgaa atcattaatg atggatcaga
     3001 attgccagtt gtgtgctata agctgaaaga agatcccaat cgcgaatgga cactatatga
     3061 tttatctgat cgtctgttaa tgaagggatg gcaagtccca gcctacccat tacctaaaga
     3121 cttggatcaa ttaattattc aacgcttagt tgttcgagca gactttggaa tgaacatggc
     3181 tggtgattat gtacaagata tgaaccaagc aattgaagag ttgaataaag ctcatattgt
     3241 ttatcataaa aaacaggatg tgaaaacgta cggctttact cactaaaatt cttcagagaa
     3301 atataaaaga agttaattaa ctaataaaag gagttgtgat taccacacag ctccttaaat
     3361 ctatttgaat aaatatatga tattcatttg ttacactctc aatcttagct gttgttctat
     3421 tcatcattac tcttattgta agtagttcag tcaaatttgc ttgttcaatt gatcgataac
     3481 agtatgaatt gtttgaaaag taatcggata tataattgtt ttctcactac ctaagccttc
     3541 ttggtataaa gaactatcgc aaagtataat ccgctgagta caaaagaaag tgtcgtctac
     3601 tatttctcta agttgtttac aaatgatatt actaatagaa aagtgttctc ccaaaatgtc
     3661 ttctaactgt tgtcgatagt ataaatacct aaagtctgac ggagctacta tgaacacttc
     3721 aattggagat ttccgtttat gctcatttaa aagacttaaa ctaatggtta gtttacgaat
     3781 agaattctca ttccaacgca gaaataaatt gttttgtttt cgccattgtt caagaatttc
     3841 atgaacttct aagtacaaag cgcgttgctc ttcatttaac aagtaagtct tatcaggttg
     3901 aaatatctga atatcagccc aaagtgagcg gataaacggg agaagtgctt tatcaaaaag
     3961 tatctgattt tcttcagata tattcagaga taagataagt ttttgactga gctcaatcac
     4021 caaagggtta tctgctacat ggttttgata aacaacgttc aagtcttttt gtaacaattc
     4081 aaagttatcg catagataat ttctagagtt gaatagaaag tagatataaa ggcattcatc
     4141 ttcgtcatga cataaagaga aagaaagttg ctctaatcct ttctgtatta gctgaaacaa
     4201 gggagtatgt cgggcaactc ttttttcttc ctgactaaaa tatattgggt tagtcttaga
     4261 tctctgcaaa ccgatagcga tacctaataa aataaattgt ttttcaactg tagggtaact
     4321 tcttgaaaaa aaatgttttt ctacataatc aatcaattgt tgaccgcttt gaataattga
     4381 tttatttagc tggatcaact ctgtccgatt tgtctcatag attatcgtta gtaacaaact
     4441 tcgataatcc ttttcaggaa tttcaaaatg gccatcagca tttaagtact gcatttgttt
     4501 aaaaaaatta atcaactcat ttcgtaagtt gtaaactttg cttaacgaaa tgaaggtagt
     4561 atcagctata ttactccaat tgtgttcccc tgataaataa tgtcctagta cattgaaaaa
     4621 gtcagagcgc tgatagatga gttgagtgag tgccaacctc gaaaacgaag agtcgaatgc
     4681 gactgtaaag ctacttctag atttctccac tgaaataatt tgctgatgga gtatgtcggt
     4741 aatgcattcc aagtcgttag tgacagacag tgagtgggca aacgttcaat tgatctgcta
     4801 tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa
     4861 aaatactttt ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa
     4921 ctattataac tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta
     4981 taaaataatt agatggggta tttggtttaa gtatccctta tatagtaata aatttatagg
     5041 tgtgattctc gaattaatac ccgaaaatgt gagagaattt ttatggattt tttaggatac
     5101 aaattaatta tttttgcttt tatcctgatt accagtccag tactgtcggt atcccatttt
     5161 gcagcaaaat catgttgata aagtgacaag aagactgatg agatatatta tccaaattga
     5221 gaagataatt aatcggattc ttgagtatac cctacttttt aagaaagtag ggtatacaag
     5281 cgtatgaata aagacataac tttccctgtg atggacgttc ggcttgttcc aattaccaaa
     5341 atacaggcga acgattataa tcccaatcga gtagcgtctc cggaaatgaa cttattggag
     5401 ctttctatca ttgaagatgg ctttactcaa ccattagttt gttattacaa ggaaaaagaa
     5461 gagaagtata tcttggttga tgtgcttcat aggtatcgtg ttgctaagga aagactcaac
     5521 ttaaaagttg taccggtaac agttattgat aaacccatat aagaacgtat ggcttcaaca
     5581 attcgacaca atcgtgcaag agggacccat gaaataagag gaatggaaat tgtcatccaa
     5641 agattagtaa aaaatggatg gtcagacaag agaatatcca aagaattagg catggacatt
     5701 gaagaaattt ttcgctttaa gcaagtgtct g
//
LOCUS       DASJOX010000145         5561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJOX010000145 DASJOX010000000
VERSION     DASJOX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.149"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..230)
                     /locus_tag="U4A12_002512"
     CDS             complement(9..230)
                     /locus_tag="U4A12_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774847.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(227..517)
                     /locus_tag="U4A12_002513"
     CDS             complement(227..517)
                     /locus_tag="U4A12_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774848.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(514..1698)
                     /locus_tag="U4A12_002514"
     CDS             complement(514..1698)
                     /locus_tag="U4A12_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1774849.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1880..3283)
                     /locus_tag="U4A12_002515"
     CDS             complement(1880..3283)
                     /locus_tag="U4A12_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1774850.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3305..4078)
                     /locus_tag="U4A12_002516"
     CDS             complement(3305..4078)
                     /locus_tag="U4A12_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774851.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4088..4465)
                     /locus_tag="U4A12_002517"
     CDS             complement(4088..4465)
                     /locus_tag="U4A12_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1774852.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4486..4800)
                     /locus_tag="U4A12_002518"
     CDS             complement(4486..4800)
                     /locus_tag="U4A12_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1774853.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5000..>5561)
                     /locus_tag="U4A12_002519"
     CDS             complement(5000..>5561)
                     /locus_tag="U4A12_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1774854.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1646 a         1096 c          939 g         1880 t
ORIGIN      
        1 aatatctgtt aagcaccaat gattcggtta aataagttta ataacacatc tttcacgcca
       61 gctgcattga acacaagtcc gacggcgatt agagcaataa tcaagaagcc aatgagtttt
      121 gaaaattctc gtttaaaacc taaaaacagt ccaatgacaa caatcgctaa caataccaat
      181 gactgtgcat tatccaagaa ccagttgtac aaattctgcc caaaattcat gtgattgtcc
      241 tcctttcctt aagatatttt tctcgataat agatatagct ttttacacgc cttaataatt
      301 gaaaaggggt taaatgctct ccatagacgg ttactcgatt ggtataatta ttataaaatc
      361 tcaccttgta aaaatcatag gtatagatgg tatcaccagc ttgccgtttt ccctcaataa
      421 tgagagacgt atatttccct gtattttgta tatacgcttg aaactcaatg ccacactgaa
      481 accctaactt ctttaattct tttgttaaaa ggctcatgta atcaaatcct ccatggctag
      541 atgttgttgt tcaattaatt tctcatgttt ttctgtcagt ttggcttcct gaatcatggt
      601 ggagataatg gttgtaccgt tgagaacatc taaaatgctt gcgattttta atgtaggggc
      661 gacttgatga tgtaaccagt tcaatgttcg ttcaaaagaa taaggctctg gctgtgtggt
      721 taggcgtaat gctccacgat ttttaccgat aaaccattcc caacgttcat tagttttcca
      781 atcactccga cgtttagtgt cgtctttatc tgcaaagcgg atatagcgat tgataatttc
      841 aaaggctgtt ttttccgcct gttgatgtgc caataaatct ttcatggctt gggtggctcg
      901 gtcatttttt aaccgaattt caaaccgatt ctttacttct gcgtcttcta agggaacatc
      961 atttttcacg tattgctcat aatctttctc atatatacag aaatagactt cactttttaa
     1021 tgagccaata taaagtgtgt ttcccatgta tggtttttca tcacggtgaa ccaattcacc
     1081 actgcgataa tttttaaagc ttcgaaagac ggaaatgcat tcttcctgtt tacatttctt
     1141 tgccaaatct ggaatattca aaatccctgt tttatcgttg atagctaaat ccaagcgttt
     1201 gaaaatacct ttatgttcca tgcaatcttg aaagaaatca taccagctcc gatgttgagc
     1261 caatagaaag ttttcaaatt gccgacagcc ttttcctttc agttctaata aaacaccttt
     1321 acttacatca tgagagacta ggacaacaat atcaccgaaa cgatagtgtt cgggataaga
     1381 ataatagcca aagtcttcgt gtagcatata atccatattc aaacgtaaaa cctcaccgat
     1441 aatttgagcc acatcattgg tagggaatcg aatccgtaca tagtcgaata acatctccaa
     1501 tgggttatcg ggattgaagc gttccaaagc gttcagtaaa tcttctttca cctgttgagt
     1561 agggactgcc ttgcctgttt caatatccga taggtaaggt ctggtaatat gagaagcaac
     1621 cgccagttta ttttgagaaa cgccataagc cagtcgcttt tctttcaatt ccttaatcca
     1681 aattttttcg ttttccattc gcaaacctcc aatctaaaaa agatgtaaac tatgaccctt
     1741 gagtttacac ctttcatatt ttccgcaatc ctttgtacca tcaacattgg ttgatgagaa
     1801 ttgcctgttt tcagttgtat ttgtgccccc ctgttagata gagggggcta aaggattggc
     1861 gtggctaacg ccacaccaat caaagcaagt ctgaacctgt acctttcact tcgtgcggta
     1921 caggctcaga ttgctttttt tgaatgactt ttcctatttc tcctaagaaa tcgtaccttt
     1981 ttggtacaag gggtgtataa aactcactaa tcacactttt tcctgtatcc acgtaacctc
     2041 ttcccttgat gggttttaag aaaaattgtt tttgtgtatc gcttccgaac atcataccaa
     2101 agcccatttc agacatacga cccaaagcca cacggaagtt aaagttgtcc cgaatcccat
     2161 cactaaaata ttttgcgtct ggacgttgac aggcaagaat taaaaagaag cctgcttgtc
     2221 gtcctaacat tacaatttgt ttgagtttat gaataacggt ggtactttca cgacctagca
     2281 tatccatcat tgccacatat tcatcaaaaa taagaaaatt ggcagacaaa ccaagataag
     2341 cataattttc tcctgtttta tagttagggt gttgtttcat ggtttcactg cgatcaatca
     2401 tatcttcgta aaattgattg atacatgcca acatatcttc tttacggtaa tagacattat
     2461 tcatgaccgc ccctaagtct gccaaatcag catttttagg gtcaagaata taaagctttg
     2521 catttgtatg caggagagat tcaatcaatg tgagaataaa ataggatttt ccactaccag
     2581 tgcccccagc aattaacatg tgagggagag aatcgaactc ccaatagacc gattccatca
     2641 atttgacacg tccatcttga gctaccacgt cttcaatgcc aattcgattg ccaatagtat
     2701 cataaagtag gacatactct acataagaat ctttcaacgt tttttccact aattcacaat
     2761 acaatcctgt ttctagtttg ttttctaggt gcaaaagctg atcttgatat ttccctaagg
     2821 tgatttctac atgaatatgg attagtccat ctttcattcg ataatatatt ttaggaaaat
     2881 ggcggattcg ttgtttggtt ttcttgctgg gtaaatcttt aaagaatccc tctgattgag
     2941 tttcttcggc ttcataccaa tgattttcta aaatcatacg agctaacttt tgacggtgct
     3001 ctaactgttt tatagaatct atacgatagt gataataaag ccagctgata agtccgcata
     3061 caaaaagagc agtgagaata ctgctccaaa agtatggcgt atgaagttga tagaaaaatg
     3121 tatctaactg aactgcttgc cagttcaccg attgtaacgc tctccaatga aaagggagta
     3181 ggacgactag aaaaactacc agcaatgaaa ttagtacaaa atgatagacc aagtgcttat
     3241 cgttggcttt gattcgctct cctctgtcat gaagcaatgg cataaaaatc ctcctttcca
     3301 cctatcaaaa taattgtgta ttgtagatac gagcctgttt atcaaaagtt ggttgagaaa
     3361 tggcttcata tttctgataa gcgattcgct tttcaaagcg taaatttttt tctttcggat
     3421 aataatgacg tcgatagaga gtgtgtccta acagtttttt cgcttctttt gtcaacggat
     3481 agagatagcg aaacatgcgt ccattgattt tctcaatccc tttgtattcg caaaatgcct
     3541 gggaaagcca atgctttttc tctacctgtt caaagcgagc attttcctct aataaaaggc
     3601 gagcacttcg agggtgtatc ttttcccatg actgcttatc acgatagaca cttgtcttga
     3661 aatacccgca gtaaaagaag ttggacgctt gatagacata gccacatttt ccctcaattc
     3721 catctgccag tgtatataaa aaatgacagt ccgtatgttc tttcaaccac ttaattagag
     3781 ctgaaagagc ttgactgcca aaataattgg tttgattcat ttctggaaga aaacacatct
     3841 ttccaatttc taaataatct gtggtttgaa gggaactgtc gggaaagagt ttgcgtatgg
     3901 tttgtagggg ttgcgtaccc cagcctaatt ccacaacgcc taataatttt tcttctgaaa
     3961 aaatccctag aaagtattta cacaatctag ggagtatctt tgaataatgg tattgccgaa
     4021 taaaagaaat ggcttcatcc ttcggaattt ctttcaaaat caatggtgtg tttgtcataa
     4081 gcaacaccta tttttcactt tcggacgctt cttttttata tttctgatga ggttttgtct
     4141 gtccttttaa tacaagatcg tctgctttca cgtaccaatc aacgtctgcc cctcgaaaag
     4201 tagccgtcgc tacggtatct gccacaggat tgattaattc cacttctgcg ttgtagtcaa
     4261 attctttcaa aggaacagaa gcggggatac tgacttgtat catgcgtcct tgtccttttg
     4321 attttaagtc ataggtacgt tctttaattt cttcggaaac cgtaccatct tcattctgga
     4381 tacgaacctc acgacgcaag gcggaaaatt tcaatgtgcc aaatgtctgg tctttttcaa
     4441 tcacaatacc ttctgctaat ctcatatttt ttcacctttc ctttcttatg ctttcaccat
     4501 atcgtctgcg tgcataatgt aattggtaaa tcctcgattg ccaattttat aaccttctgc
     4561 ggtaatatgt gggttgatga gtttgacttt ttcgtcggat tcaaaatgtt tctcgccagc
     4621 ttcggaggga agaatgacaa caatatcatc tgctcgttga atcgttgagt ataaattgta
     4681 actacgagag agaatggttg gtctgccatt tattcgacgt tgttcaatgt tcccttcacc
     4741 agcatattct aattgtccaa atgttttttc catattggga atgacatatt taagttccat
     4801 atttttacct atcctttctg tttttatgtt tgaatgaatc ttatttgtgt gatggtttta
     4861 aaggggggag agcgaccttt tgattcttga ttttttattt tcataagttc acttcctttc
     4921 aaaattgggt aaaaaaatag acgctccgta ctgagcgtcc gattcctact atatttaaaa
     4981 caacgactac ctaggtattt taaatattat aatcaaattc tattttatct aataattgag
     5041 tgggataaag caagtctcct ttactcgtaa aataacccac ctctgagaaa agaaataaat
     5101 tattttttgc tcttgaacaa attacgtata gtagttttct cgctgaacta attcctttat
     5161 cagaaacatt aaaaatttca ttccaatgag gaattttccc gtttagaaga ttaatagcga
     5221 taacagtatc atactcattt cctttaactt tatgtgctgt agtaagtgtg ataccatctc
     5281 gagatttaaa catcttctta aaactagata cactactaga taatgaatta ttttttatcc
     5341 gatcttctac ttcgttaata aaaaatagat agtgcttaaa aatagacggg taatttttct
     5401 cgtcaatatt aagattaaca aatatatgtt tggtaacaaa tttgtataaa tcaacaccgt
     5461 tgtcgtctcc actatacatt cttttcacac tgtttattag ctgtaataaa tctaagtcaa
     5521 taaaataatc tttaacttct attccaaatt cattttttaa t
//
LOCUS       DASJOX010000146         5534 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJOX010000146 DASJOX010000000
VERSION     DASJOX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.150"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..988)
                     /locus_tag="U4A12_002520"
     CDS             complement(<1..988)
                     /locus_tag="U4A12_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN1774855.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="U4A12_002521"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="U4A12_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1774856.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="U4A12_002522"
     CDS             1813..2967
                     /locus_tag="U4A12_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1774857.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="U4A12_002523"
     CDS             3182..4150
                     /locus_tag="U4A12_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1774858.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="U4A12_002524"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="U4A12_002524"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1774859.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="U4A12_002525"
     CDS             5180..>5534
                     /locus_tag="U4A12_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEN1774860.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DASJOX010000147         5525 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJOX010000147 DASJOX010000000
VERSION     DASJOX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.151"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            160..1092
                     /locus_tag="U4A12_002526"
     CDS             160..1092
                     /locus_tag="U4A12_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774861.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1313..3076
                     /locus_tag="U4A12_002527"
     CDS             1313..3076
                     /locus_tag="U4A12_002527"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN1774862.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3160..3618
                     /locus_tag="U4A12_002528"
     CDS             3160..3618
                     /locus_tag="U4A12_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN1774863.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3727..4410
                     /locus_tag="U4A12_002529"
     CDS             3727..4410
                     /locus_tag="U4A12_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1774864.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4557..5171
                     /locus_tag="U4A12_002530"
     CDS             4557..5171
                     /locus_tag="U4A12_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN1774865.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5187..5393
                     /locus_tag="U4A12_002531"
     CDS             5187..5393
                     /locus_tag="U4A12_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774866.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1978 a          863 c         1123 g         1561 t
ORIGIN      
        1 atcgtattgg atttttttaa agaaaatgat tatttgaaaa ataaagaaat cattatccca
       61 gaatttgact cgcggaacat cttgctagct ttagtacgtt atgtcaattg ggaaggctat
      121 gaaaataaaa atccgtatgt agaagaagct tgtaacgagt tgattgatta tgtagagact
      181 cattttttta aaagaagata ttcggaagat gaaaagtcct ttatcatacg aggaatagag
      241 atcgcaatcg gaagaaaggc atttcccctt ggactgaaag aagaagataa gaaaagcgca
      301 gaaacgaagc cgttatttca attagtctgt caaggattgg aagagcaaaa aggaattcta
      361 gacttacaag aagaagatgt ctattatatt ttttctttgt tcaacagtag aaattatacc
      421 aatgagaaca tggagctgtt aagaaaagat ttcgaagttg tttataaaaa ttttgttctc
      481 aatgacctat cactgaatga attgatttta cagattatta gtatcgctga aatcgacgat
      541 gcagaaaatt ttattttcaa gcaatccttt ttacaatttg tccgtacgtt gtgggcagat
      601 ggacaagtat ttttaccgga aaggatctat cttctatcag aaaaagaaca acttctgtac
      661 ggaaaaattg tagaaatatt ggaagagtgg aaaaagaaaa atcaaatcca actaagatgg
      721 aatgaaaatt tcattcgaaa attcgtcaaa agtctttctt tgatcaacag tgaacaagcg
      781 gggagacagg aaatagaaat ttttgttgtt tcagaaagtt cagtcaaaca atttttttat
      841 cgaacccaat ttcatttgta tgcggaagaa ggcatcgctg aaataaatcc tattttattt
      901 cgttcattag aagaattgcc tgatgaatgt ctatgtgcga aaaaaagagt ggttttgtgc
      961 gatgctgcta gctatcaaaa tggattagaa actgaaaaga caaagatcta tccaatctca
     1021 ttaaaaggtg caaacatcca tttgtatcat attgctcagg aacttcacct attaaaaaaa
     1081 catatttctt gatgagacct taattaggag accttctaca aagcttttta attgtctgta
     1141 gaaggttttt atttgagtcc tgattcgtgc gaagatctac ttatataaca agctttctct
     1201 gaaatttttt taaaatgcta ttgacttaga gtcaactcta agtagtagaa tagataaagt
     1261 gtaacaaatc tattttcatt aaaagaagat agtacggagg agagaatgta ttatgagtac
     1321 agtattacat gtattggatg caatcaatag ccccgcagat gtcaaaaaat tatctgttga
     1381 ggaaatgaaa cagttggcag aagagatgcg ccaaaaaatc ttgcaaaagg acagcgatgt
     1441 aggtgggcac gttggtccga atctaggtgt agtagaatta acgattgcgt ttcattatgt
     1501 atttgattca ccaaaggata aaatcgtttg ggatgtttcc catcagtctt atggtcataa
     1561 gcttttaact ggtcgaaaaa atggttttga aaaaggacat taccgtgaag tgagcggata
     1621 ttccaaccaa catgaaagta aacatgacta ttttacagtg gggcacacat ctacatctat
     1681 cagcttagca acaggtttag ctaaagcgag agatctgaag aaagaatcag gaaatgtggt
     1741 agcatttatc ggtgacggct ccctaagtgg tggcttagct tttgaaggat tgaacaatgg
     1801 tggtgtttta caatccaatc taatcgtatt agtaaacgat aatgagatgt ctattgatga
     1861 gaatcatggc agtttatata aaaacttagc tgagcttcgt caaaccaacg gtaaaagcag
     1921 caataaccta tttaaagcaa tgggatttga ttatcgttac gtggataatg gaaacgatct
     1981 ggaaacggtg atccgtttat ttgaagaagt aaaagatatt gatcatccga ttgtacttca
     2041 cgtccatacg gaaaaaggtc gcggttatca gccggcaatc gataataaaa tgaaatatca
     2101 ttggcatgta ccatttgatc tgtccaccgg ggaggcaaag caagtaactt ctggagaaac
     2161 atttaatatg atcatcatgg attatttgga taagaaaatg caaacaagta cagctcctat
     2221 tgccgcagtc aatgcagcga taccaggcgc ctttggactg aaaacattcc aagaaaaaca
     2281 tccagatcat tattttgacg tggggatcgc tgaacagcac tctatcactt tcgcttctgc
     2341 tttggcttct cagggagtac gtccagtcgt cttccaatca agtactttct tgcaacgggc
     2401 atatgatcaa ttatcacatg atttagcgat caacgagaat ccagtgatta ttttagtcgg
     2461 tggcggaacg atctcagcta gtgaccgaac acaccaagga acatttgaca ttccttactt
     2521 atcaagtatc ccgaacatca aatatcttgc tccagcaaca aaagaggata tgatctctat
     2581 gctagattgg gcgttcgaac aaacagcgca gcctgtagca atccgaattc ctacacatgg
     2641 tgtagaacat ggtacagcta tcgcaaatga ttatagtatt ccagcctatc aaacagtaaa
     2701 taatggagga aaagtagcaa ttttagcttt aggcggtttt cttgaattag gaaaagaaac
     2761 agtaaaagct tttagaaaaa aaggaatcga agcaacgttg atcaatccag gaacgatcac
     2821 agaaaatgac gtagaaacat tagataaatt aaaagaaaca cacgaattag tcatcacatt
     2881 agaagatggt agtttgtcag gaggatttgg cgaaaatatc agtcgttatt atggtgattc
     2941 ttcaatgaac gtattaaatt ttggtgcaga gaaagaattc acggatagcg ttcctgtagg
     3001 gcagctttat gaacgttatc atttaacacc aaaacaaatc gttgaagata gtctgcgtgt
     3061 cttgaatatt caataattca ttatgctcac catccagata attgtttata ctagtatctg
     3121 gatggttttt tcattataaa aagcaaaggg gtggatgtta tgtatcagca agtagaagaa
     3181 tatgtcaaaa agttccaaga aaaatatcct agttatcaat cagaaaaatt aggtttcttt
     3241 tcatttgggg atacagaaaa aatggcagac gagttgagcg aactagttgt aaaaaagata
     3301 aagactggta catcaagtgg ttacgatctg tataaacaag agaatgagcc tttaccaaaa
     3361 gttggtcaat tagacgtggt cctgaatgga aaaaatgaac cagtttgtat catcaaaaat
     3421 ataaatgtga ccatcttgcc ttttagtgaa gtcacgaaag agcatgcttg gaaagaagga
     3481 gaaggggatc ggacattagc ttattggcgg aaagtccatg ctgagttttt taaacatgct
     3541 tataaagaag cgttgaacat agaatttaca gaagagaagc tggtcgtata cgaagaattt
     3601 gaagtaattt tcttttagta agaaaggtct gctgaaagaa atagctcatt attgtctcct
     3661 gaatagaaaa gggttacatt tttatatgaa gctgtatttt tatataacat tactggagga
     3721 gatattgtgg atacactgga acaaatcaaa ggcggagtcg tcgtttcttg tcaagctttg
     3781 gaaaacgagc cgctacacag ttcttttatt atggggagaa tggctttggc tgcaaaagaa
     3841 ggcggagcag ttggtattcg agcaaatacg acagaagata tcaatgaaat caagcgaatc
     3901 gttgatttac cggtaatagg gattatcaaa agagattacg atgggtcaga agtgtttatt
     3961 actgcgacag aaaaagaagt ggaagaactt ttggagacgc aagcagaaat cattgcactg
     4021 gatgcaactt ctcgtaaacg tccgacagaa aaaacaactg ctgaacttgt ccaaatgatt
     4081 cacgaacacg gtcggttagc tatggcagat atttcgactt ttgaggaagc gcttcaagcg
     4141 caagaagatg gttttgatct tttgtctacg actttagcag gctatacaga atattcacga
     4201 aagacgcaga cacctgattt tgatttattg aatcaaatca tagaacaagt cgatatccca
     4261 gtaattatgg aaggacatac tgactctcct gaacaagtag agaaagcata tgaattaggt
     4321 gctttttcag tcgtagttgg atctattatc acccgtccgc aattgatcac taaaagatat
     4381 gtcgaagccg caagtaaagc aaataactaa aaaacacgca cggcacagat tcttgacaaa
     4441 aaagaaaaat tgatagatag tttaaagcag attctaagaa gatgaaaaaa gcattacttt
     4501 tcattttgtc ttcaagatct gctttttaac gaattttata gaaagaaggc gaaaccatgt
     4561 ttgaacggag tccttcagaa ttgacagaac gtgaaaatta caagttgctg attggttcag
     4621 tcatccctag accagtagca gtagtaacga ctaaatcagc aaaagatata gtgaatatcg
     4681 cacccttcag ttacttcaat atcgtcagtt cgaatcgacc catcttatcc ttggcgatcc
     4741 agagaaaaca agatgcagta aaagacacag caaagaatat tcttgagaca agagaagctg
     4801 ttatccatat ccttgatgag gacaacgtag aagaagtaaa taaaacagca gccaatctgc
     4861 ctaatgagga aagcgaactg tacctgactg acttgacttt aactgattca gtaatcattt
     4921 cagtaccagc attgacagaa gcaaaagttc gttttgaaac atccttatat gaacatgtcg
     4981 agatacggaa taaaaataaa gtaacagctg atttattttt gctagaagta cagaaatatc
     5041 atttaaatga aacagtctat gatgataaga caggaaggat cgatccaaga aaattgaatg
     5101 ctgtaagccg tttggcagga aatgattatg cagcaatagg agaaattttc acgattgcac
     5161 gtcccgaata aaaaggagca ctagttatgt taaaaataag ctataaacca tcgacagatt
     5221 caaaagaaat gaaaaaagaa tatgaaacag tgaatgattt tctgcaaggg cagtacttag
     5281 aagttccgcc attgcaagac cattttgtcg ttactactgt tactttagat ggtaaggaaa
     5341 tagaaatgcc agatcaaacg attagcggat tgtttaatta ctttaacaaa taaatattta
     5401 ttccgaaaag tgaaaaaaca tataaaaaaa cacataaaac taaaaaatta tcaaagaatc
     5461 ctggtgcaac tgaaagaatt tgcaacggga ttctttcaac ttatttgtta tttttttcta
     5521 aaaaa
//
LOCUS       DASJOX010000148         5450 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJOX010000148 DASJOX010000000
VERSION     DASJOX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.152"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            326..883
                     /locus_tag="U4A12_002532"
                     /pseudo
     CDS             326..883
                     /locus_tag="U4A12_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            941..2980
                     /locus_tag="U4A12_002533"
     CDS             941..2980
                     /locus_tag="U4A12_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN1774867.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3089..4240)
                     /locus_tag="U4A12_002534"
     CDS             complement(3089..4240)
                     /locus_tag="U4A12_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1774868.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4394..4924)
                     /locus_tag="U4A12_002535"
     CDS             complement(4394..4924)
                     /locus_tag="U4A12_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1774869.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5023..5373)
                     /locus_tag="U4A12_002536"
     CDS             complement(5023..5373)
                     /locus_tag="U4A12_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN1774870.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         1968 a         1005 c         1031 g         1446 t
ORIGIN      
        1 atagaatata gtaataacct tgttaggtga ggctcctata caaacatagg ctgttgccca
       61 gaaacgtcga aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc tttttccaat
      121 agctaagaat gttttcttta cgttgtatag tgctaaaact cggacgatga gatgatgcta
      181 ggtcagaatt cttgttcgaa aaggtttctg accttttctt tatatttttg tgactgactt
      241 atttgatgag acaaactcgc tcctcacctg acaagtggat tgctgtgtga ggaggagatg
      301 cggtgaaaaa agatcgattg gtggaggtcg ctcggattca cttctggttg cacaatacaa
      361 ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg tcgatattcc
      421 aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg atttattaaa
      481 tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta tcacatttca
      541 agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa
      601 atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg atggtaatac
      661 tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga ggatcagaag
      721 gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt caatattcaa
      781 gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc cagaaagtgt
      841 gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac aggtataaat
      901 agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca
      961 cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag cagctgcggg
     1021 tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag ctggagaaaa
     1081 gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag ctgcagaaat
     1141 gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct tagataaata
     1201 ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc taaaagtaga
     1261 taaaaaagac gattctactt acagtttttc atataaagca aagatgaata cctcattagg
     1321 tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc aaaccacgat
     1381 caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag taagcctgac
     1441 cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat tagcaacaac
     1501 aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac
     1561 agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta tcaatcaggc
     1621 gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca ttgatggagc
     1681 aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat attatccttt
     1741 gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag aagatattga
     1801 taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt
     1861 tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg cagacggtgt
     1921 agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca aattgacaat
     1981 cgatgcgaag gcacaaaaaa tagcttttga cagtctagga ggaaaagctg catcaactgt
     2041 tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa gctatgatcc
     2101 aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag aaaatcctga
     2161 acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat
     2221 tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt tgacgatcaa
     2281 cgggcttaaa tggcaaaaag acagttcttg gggatctttt aaaacaacgc gtgtcagtag
     2341 tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata tatatgcggc
     2401 acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata aattcatttt
     2461 tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta atgaagatag
     2521 ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac ttctaattac
     2581 tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac ttgtctatcc
     2641 taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg aaacagcagt
     2701 acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta cagcacattc
     2761 tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa
     2821 acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg ataaccaagg
     2881 ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa ccaaacgagc
     2941 atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca
     3001 gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt tttcacgatg
     3061 tctctttcac aacttctttt tgatttactc acacttcata aacttttttt gtaaaatatt
     3121 tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa ggtgaatagt
     3181 actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa gccatttctc
     3241 cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa ccggcaatct
     3301 gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa
     3361 taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa
     3421 atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact tcttctttaa
     3481 cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag ctaagcccaa
     3541 caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg ataatagaag
     3601 aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata
     3661 agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa gaagcattgc
     3721 tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg actaaaatgt
     3781 catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact acgtttagtt
     3841 cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc ccaaagaaaa
     3901 tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga
     3961 agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc aaaagagata
     4021 aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc gaaaagtcat
     4081 ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa acaccaacaa
     4141 gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag ccaatcgttg
     4201 ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt cctttgtata
     4261 aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa tatactcaca
     4321 aaaaaaccaa caatccaaaa gatttttttg gattgttggt ttttatacgg tctattttaa
     4381 taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct
     4441 ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga
     4501 atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc
     4561 aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag
     4621 ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca
     4681 gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc
     4741 ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga
     4801 tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa
     4861 taatataaac agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa
     4921 acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa
     4981 atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt
     5041 ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt
     5101 catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga
     5161 acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg
     5221 tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac
     5281 ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc
     5341 aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat
     5401 tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc
//
LOCUS       DASJOX010000149         5393 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJOX010000149 DASJOX010000000
VERSION     DASJOX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.153"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(202..660)
                     /locus_tag="U4A12_002537"
     CDS             complement(202..660)
                     /locus_tag="U4A12_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1774871.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(673..2004)
                     /locus_tag="U4A12_002538"
     CDS             complement(673..2004)
                     /locus_tag="U4A12_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN1774872.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2015..3457)
                     /locus_tag="U4A12_002539"
     CDS             complement(2015..3457)
                     /locus_tag="U4A12_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1774873.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3444..4208)
                     /locus_tag="U4A12_002540"
     CDS             complement(3444..4208)
                     /locus_tag="U4A12_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN1774874.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4312..5187)
                     /locus_tag="U4A12_002541"
     CDS             complement(4312..5187)
                     /locus_tag="U4A12_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1774875.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1663 a         1121 c          775 g         1834 t
ORIGIN      
        1 atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa aataagtttt
       61 ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat gattaaatct
      121 agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa tatcacccta
      181 tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct ttacttcttg
      241 gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga tcaccgtatc
      301 tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc cttcttttac
      361 ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa ctgtatctaa
      421 acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg catgtcgggt
      481 aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat catgattcgt
      541 tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta ctgctgttaa
      601 tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa agaaatgcac
      661 tttttatcac ctctacttta atgctggcca atattttttg tttactttga tcatttcatc
      721 tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa atgcttgtaa
      781 agctttttga taagaacctt caaagtaagc atctactagt aacttctcta ctgcaacttg
      841 tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa ttggctccac
      901 accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta gattcggaat
      961 cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca acaaggaaat
     1021 agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata cttcttccac
     1081 aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc catccatgac
     1141 catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag gatataagta
     1201 atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga ctgacatcat
     1261 ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt gcatttcttt
     1321 tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt tatcataaat
     1381 cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat tcttacgatc
     1441 ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac aggcattaat
     1501 catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact ctggatttgt
     1561 ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa tttccaataa
     1621 acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt cttgtcctac
     1681 taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc ctccaacacg
     1741 aatttgtgag aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat ccgttgcacg
     1801 aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca agtacatatc
     1861 catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca accgtttttc
     1921 attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc cagctatagt
     1981 gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat ttattgaatt
     2041 ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt tcagttggtt
     2101 taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg gtgggatcca
     2161 ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg gcttttcctc
     2221 caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct aaccctaact
     2281 ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt cctaatgtct
     2341 gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca aaaaagaaaa
     2401 ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg agataatcaa
     2461 caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag acaatccctg
     2521 ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac atgaaactaa
     2581 attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt cccatgattc
     2641 ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga catatagctg
     2701 gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc tgaggatctc
     2761 cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat gtatagcttc
     2821 ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg atcagcaaac
     2881 gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc agttttgcaa
     2941 aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca gctgcgattg
     3001 tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa gaaaaaacta
     3061 ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa ccggaaataa
     3121 ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct tttttcaagg
     3181 tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt ttgacaaaac
     3241 cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca tttccagctg
     3301 caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc aatcccgcta
     3361 atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca tttgatttaa
     3421 cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc aatcaaagga
     3481 tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg gtgggtacga
     3541 ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc gtgacacatg
     3601 acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata ttcttccaca
     3661 ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa acaaggctca
     3721 aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac cggcaaatca
     3781 tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg agtatgatga
     3841 tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat ctgagcattc
     3901 cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata agataaggat
     3961 ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt gccacacgtc
     4021 aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt cacagcatgc
     4081 tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg ctgataagca
     4141 tccataatcc cataattcac accatggcta tagccaaaat catccgaatt aaaaatcact
     4201 tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa taatcatagt
     4261 ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat tttaatagat
     4321 tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa gttgaaattg
     4381 ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat gtttaaatgt
     4441 ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca agcgtttaac
     4501 caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat cataaacgtt
     4561 tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta tttttaagct
     4621 ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga tgaatcctac
     4681 ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca cttctactgc
     4741 tatatcatat tcatttggat aaaggaatct catttctggc aacataattg ctgtgacatc
     4801 tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg taatatagat
     4861 atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt cttccgcaaa
     4921 atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga tcttttcttt
     4981 agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac tcactttagc
     5041 agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat cgatcaatac
     5101 agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc tatatgataa
     5161 aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat ttttaaagac
     5221 aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata gtagcaccct
     5281 acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc ttttttactc
     5341 gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa aca
//
LOCUS       DASJOX010000150         5291 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJOX010000150 DASJOX010000000
VERSION     DASJOX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.154"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(406..780)
                     /locus_tag="U4A12_002542"
     CDS             complement(406..780)
                     /locus_tag="U4A12_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1774876.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="U4A12_002543"
     CDS             complement(803..2203)
                     /locus_tag="U4A12_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN1774877.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="U4A12_002544"
     CDS             complement(2207..2617)
                     /locus_tag="U4A12_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1774878.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="U4A12_002545"
     CDS             complement(2635..3126)
                     /locus_tag="U4A12_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1774879.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="U4A12_002546"
     CDS             complement(3136..3927)
                     /locus_tag="U4A12_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1774880.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="U4A12_002547"
     CDS             complement(3920..4693)
                     /locus_tag="U4A12_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1774881.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5291)
                     /locus_tag="U4A12_002548"
     CDS             complement(4849..>5291)
                     /locus_tag="U4A12_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1774882.1"
                     /translation="LENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLR
                     FLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKE
                     GAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1690 a         1064 c          694 g         1843 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa t
//
LOCUS       DASJOX010000151         5122 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJOX010000151 DASJOX010000000
VERSION     DASJOX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.155"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..620
                     /locus_tag="U4A12_002549"
     CDS             414..620
                     /locus_tag="U4A12_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN1774883.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(822..1823)
                     /locus_tag="U4A12_002550"
     CDS             complement(822..1823)
                     /locus_tag="U4A12_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1774884.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1828..3741)
                     /locus_tag="U4A12_002551"
     CDS             complement(1828..3741)
                     /locus_tag="U4A12_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1774885.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3909..4415)
                     /locus_tag="U4A12_002552"
     CDS             complement(3909..4415)
                     /locus_tag="U4A12_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1774886.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4575..5015
                     /locus_tag="U4A12_002553"
     CDS             4575..5015
                     /locus_tag="U4A12_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1774887.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1412 a         1025 c          781 g         1904 t
ORIGIN      
        1 ttatttttgt ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc
       61 ttgataacac tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt
      121 gatatgattg ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct
      181 tttgggggta tcctaaatct tttctattat attcccatat tttctattat tggtctagac
      241 gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc
      301 atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat
      361 ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag
      421 ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt
      481 gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac
      541 cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac
      601 gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt
      661 gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca
      721 agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg
      781 agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt
      841 actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg
      901 ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca
      961 aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag
     1021 aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct
     1081 gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta
     1141 gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata
     1201 aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct
     1261 tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca
     1321 atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct
     1381 tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata
     1441 tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt
     1501 ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt
     1561 tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt
     1621 tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact
     1681 tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg
     1741 atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc
     1801 actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat
     1861 aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc
     1921 agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg
     1981 aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc
     2041 tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa
     2101 aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa
     2161 ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag
     2221 ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt
     2281 tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg
     2341 gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc
     2401 tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc
     2461 atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt
     2521 atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc
     2581 atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc
     2641 tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc
     2701 aataattcct ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc
     2761 tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt
     2821 tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct
     2881 attaccagtc gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt
     2941 gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac
     3001 tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg
     3061 tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt
     3121 aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa
     3181 agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc
     3241 ataatcatta acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc
     3301 tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc
     3361 attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac
     3421 tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg
     3481 gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt
     3541 ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt
     3601 ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt
     3661 ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg
     3721 ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa
     3781 acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct
     3841 cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa
     3901 gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa
     3961 gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac
     4021 atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct
     4081 gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa
     4141 ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata
     4201 gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt
     4261 tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat
     4321 gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa
     4381 tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt
     4441 gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt
     4501 acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc
     4561 tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac
     4621 gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc
     4681 aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg
     4741 aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata gatgaactca
     4801 gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta
     4861 tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc
     4921 tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg
     4981 attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc
     5041 tggatctata atatctagtg ttggtgacgc cttctgttgc caatactttt tcttttttct
     5101 aataaacgca aacagacacc tt
//
LOCUS       DASJOX010000152         5119 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJOX010000152 DASJOX010000000
VERSION     DASJOX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.156"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            110..532
                     /locus_tag="U4A12_002554"
     CDS             110..532
                     /locus_tag="U4A12_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774888.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(698..1891)
                     /locus_tag="U4A12_002555"
     CDS             complement(698..1891)
                     /locus_tag="U4A12_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1774889.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2115..2492
                     /locus_tag="U4A12_002556"
     CDS             2115..2492
                     /locus_tag="U4A12_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1774890.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2480..2818
                     /locus_tag="U4A12_002557"
                     /pseudo
     CDS             2480..2818
                     /locus_tag="U4A12_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2808..2921
                     /locus_tag="U4A12_002558"
     CDS             2808..2921
                     /locus_tag="U4A12_002558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1774891.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            2952..3395
                     /locus_tag="U4A12_002559"
     CDS             2952..3395
                     /locus_tag="U4A12_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN1774892.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3656..4471
                     /locus_tag="U4A12_002560"
     CDS             3656..4471
                     /locus_tag="U4A12_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1774893.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4481..>5119
                     /locus_tag="U4A12_002561"
     CDS             4481..>5119
                     /locus_tag="U4A12_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1774894.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRK"
BASE COUNT         1711 a          884 c          975 g         1549 t
ORIGIN      
        1 aagagaaata ataacaggtt tatgcactat tgacggtgtg acaatatgat atttgtatcc
       61 gtttttttct tgtatgtagt atagtaaagt agatacagga ggagaaaata tgcagacttt
      121 tttaacaaaa gtttctcaaa agaaaattaa aatgattcat ttactgcttg aaacagaacg
      181 ttggtgttca atagaagaac tacaaaacga attgaaggta tcaagtaagt ctatcttgaa
      241 ttatctaaca gatttagaag aattgttcca acaatatcct gataaggtca ttttgaagaa
      301 tgagaataat cggcgctttt ctatggaaaa gcaggagaac ttcccaatct acattattta
      361 tcttcatttc tacagaaaat catataatta tcatttgatt gaattcatgt atcaacatcc
      421 tgagaaaaat ttggaagatt atgcagacgc tcaatatacg agtgtatcca cagtttttcg
      481 ttatgctaag ttattagtaa agtactttga aatgacggac gtcaaagaat agtcgccgcc
      541 agatagaagg aaattagcag ttgaaaaagc tgccctgcat caagtaatac gaccagccaa
      601 acggaaacag tgtctcatgt ttttgatttg gctggtctta ttaccgcaga tgcaagcttt
      661 ttccacacct ctttcttgtc atttacagac tcaactatca atgctgcaag ctttccctta
      721 atgctgtaac accagtaaga gttgtcaaga ccagcagact gactaggtaa ataccgctca
      781 aaaacaagat tacggcagtt aaactataat gatccatcaa gaaaccgatg acgatagaag
      841 aaaaaccacc aacagctcgt ccaaggttca taacgaaatt attcgccgtc gcgcggattt
      901 ctgttggata caaactacta atcactgcgc catatccacc atacatgcca ttgataaagt
      961 agccgactat cacagcggct gctaccaaac tccactggct atgggctaac acaagtagat
     1021 agacagataa ggatgaacaa attaaaaaca aagaaaaggc tagtttaggc cccaatttat
     1081 ccatgattat gccaaaagtc atcatcccca aagacatgcc taaaatagta ctgaccatcc
     1141 acaatgaaga cccagaaacc gaaagcccta atcgattttg cataattgaa ggtagccaat
     1201 tcattaaccc aaaatagccg gcaatttgga cagttaccat gatactaagg ccacatgttt
     1261 gccaagcaag atgttttgat tcaaacagtc tgctaagttt ggttttcttt tcggcagatt
     1321 gtacagattt gaattccttt ggttcagtca agccgcgacg aatgaacaag accatcaaca
     1381 ccggcagtac acctacgaca tacaacatct tccaaccaaa cgttggaatg atcacagcag
     1441 ctaaaatcgc agccaaagcc gcaccgacct gcgcaccaat accagcaacc gaagaagctc
     1501 gtccaatttg tttttttgaa aaactttcgg aaatcaagga catacacgcc ccatattctc
     1561 ctcccgctcc tacacctgcg ataaagcgaa agagataaac caagtagatg tttgaagcaa
     1621 aatacatcaa taaagaagca atcgaaaaca gaaggaccgt ttgagaaaat actttcacac
     1681 gtccatattt atcagctatg atgccaaaaa aaatacctcc aatgagcatc cccaaattag
     1741 tgatcgttga aatgaaaccc gcttgcgtac cactaatatg aaaagaagta atcattgaag
     1801 ataatgaaaa agctaaaaac ataatatcca tattctctaa agcaattccc gccgatgtcg
     1861 atactaatac ctttcgctga tacgttgtca tttttatacc ccttttccta aaatttccct
     1921 aaattgttaa aagtatatcc aaaaaagaat aaaaaaacaa gactttttta tttgaaataa
     1981 tacacaaaca accgatatag ataatccagc ttagttttaa tagaaatgag tattgacttg
     2041 gagtacactt aaagttgtag aataaataaa atacataagt aaagtggcaa gaaattaaga
     2101 gaaagagtga tcagatgaat atcaaacaag tcagtgaaga aaagggcatt tcggccgata
     2161 cattgcgcta ttatgaacga atcgggttga ttccaccagt taatcggaca aaaggcggaa
     2221 tccgtgatta tacagaagaa gacctaaaat gggtcgactt tacgatctgt atgcgaaatg
     2281 ccggattatc gatcgagtct ttgattgaat atattcgcct atctagcgaa ggagaagcaa
     2341 ctgtatttga gcggcgtaag cttttgattg aagaaagtga acttttgacg aaaaaaattg
     2401 ctgaaatgca agaatgtttg gaacgtttac aaggaaaaat caagaaatat gatcgtgtgt
     2461 tatctagaaa taaaatggaa tgtatcaagt aaaaaaatca aggagcatcg ctatggagtt
     2521 acgcttacta aaatattttt ggacaattgc agaagaagga acaatctctc aagccgcaga
     2581 agttctacat cttacacaac caaccttaag ccgacaactg aaggagttag aagagtaact
     2641 aggaacagaa ctgtttgtac gcgaaaaccg caaaatgatc ttgacagaag caggttattt
     2701 tctaaaaaag ccgagcagaa gaaattttag atttaacgaa tcaaaccact caagaattcg
     2761 agaacagaaa aaaacaatta ttcagcggcc gatttacgat cggctgtgtg gaagctgata
     2821 gttcgaatac attggcacta ttactagaaa agttgatccg tgattatccc agcgttcaat
     2881 ttcatatcca cagcggaaca agtgatgata tcacggagtg actagaaaaa agcctgttag
     2941 atttagcgat tttgctagat ccagtagata cagagaaata cgaaacgatt ttgttaccaa
     3001 gaaaggaaaa atggggattg ctcattttag aaaattctgt tttagcaaaa aaagacgtga
     3061 taacaaaaga agactttgcg aatgtacctc ttttgatctc aaagcgtcca gaaatacagc
     3121 gaatgtttgc tgactggtta ggcataaaaa aggagcagtt gctgatcaca ggaacgtaca
     3181 atttgatttt caatgtattt gcgctaattg agaatcaggt aggtgctgct ttagtcatag
     3241 aaggtgctgt aagtcatcat tcaaagcgag tgacattcgt tccgcttgac ccaatgattg
     3301 aaacgaactg catgctagtc tggaaaaaga atcggatttt atcacctgta gtcaatgaat
     3361 tgattcaccg tttcaaagaa gcttttgaac agtaataagt atctctaatc gaagaaattt
     3421 caatacaaaa gcgagacgta tgacagtctt gcttttgtat tttttcattt ttaaatcaaa
     3481 aaaataaaga gatttacata acttgttaaa taagcgctta caaaaataag aagaacggaa
     3541 atgaatcagc tataataacc gttgttctgg attgtaatca ttacaaagaa atgaagatta
     3601 taactttgga ggtgttcgaa atgaaagaaa aaaagcaaaa attcgttcaa aaaacatgcc
     3661 ctttattcaa tggttgggta tgattctgct gattgtatta gctttattga cgatcattcc
     3721 ttttttatgg atggtttctg cttcattcaa agcaaataat gaagtgttca cgattccgat
     3781 tcaatggatc cctaaaactt ggcatttaga gaattatctg gttatttggt ctcgtattcc
     3841 tttagtgaca ttttttaaga atacagcttt tttaagcgtt gtgatcacgt tgattcagtt
     3901 gtttacttct agttttgctg cttacgggtt ttctaaacta aattttaaag ggcgcgatat
     3961 tttattttta gcatatatcg gcacgattgc tgtcccttgg cagtcttata tgatcccgca
     4021 gttcattatg atgagaaaag tgggtttaac agatacgttg tggtcgttgg tgctattaca
     4081 agcgttcaat gcttttggtg tgttcttgtt gaagcaatat tacagtagta tcccagattc
     4141 tttatgtgag agtgcgcgga tcgatggctt atcagagtgg ggaatctatc gcaagatcat
     4201 tttacctctt acaaaaccgg cgattgccag tttgacgatc atcacattcg ttaatacatg
     4261 gaacgattat atggggccat tcatttattt gtcttctacg gaaaataaaa caatccagtt
     4321 agggttgaaa atgtttattg ggctatttga tgcagagtac gcattaatca tggctgcctc
     4381 agttgtttct gttcttcctg ttgcttttat atttttagcg atgcaaaaat attttgtaga
     4441 agggatcgct tcatcaggaa tgaaggaata ggaggtgatt atgaaagagc agcgttttga
     4501 caaaaatgtt tatatggaat tcttttattg gttgtatcaa tcattggcaa gtagcctttg
     4561 tttttggtgt gtcaatatct tgtatatagc agctgcgttc cttttgtcga tagatggaag
     4621 gaatgtcttt tcctttattt tttctttact ttttattggt ccaggttgca tcgtgatgat
     4681 aggtatgaca gaagaattag caaaagaaaa tagtttttca ccagtacgtt tgttcataaa
     4741 gatgtttcgc caattttggc taaagggatt tttttattgg ctatttgctt ggatagttag
     4801 tgtgataatg atttttgatt gtttcttttt tatacgtttt tcttatggaa aatggttgat
     4861 tccgttgttt gttttacttg cttgtttatc agtttccttt agcaccaact gctggtattt
     4921 tcaggtgaga aatccagcgt cgaaaccaaa tcaagttcta cggatagcgt tctactatac
     4981 gttgaaaaaa tggtatgtga gtttactaga ttttttacta ttgacaagtt tgtttctctt
     5041 ctttttcgta aagccccaat ggtgtattct actaggtcct tctatcgtat ttggtttgat
     5101 ttacttcaac aatcgtaaa
//
LOCUS       DASJOX010000153         5085 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJOX010000153 DASJOX010000000
VERSION     DASJOX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.157"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            120..938
                     /gene="purR"
                     /locus_tag="U4A12_002562"
     CDS             120..938
                     /gene="purR"
                     /locus_tag="U4A12_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN1774895.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1441..2814
                     /gene="glmU"
                     /locus_tag="U4A12_002563"
     CDS             1441..2814
                     /gene="glmU"
                     /locus_tag="U4A12_002563"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN1774896.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2908..3879
                     /locus_tag="U4A12_002564"
     CDS             2908..3879
                     /locus_tag="U4A12_002564"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1774897.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3885..3987
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4053..4622
                     /gene="thiT"
                     /locus_tag="U4A12_002565"
     CDS             4053..4622
                     /gene="thiT"
                     /locus_tag="U4A12_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN1774898.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4703..>5085)
                     /locus_tag="U4A12_002566"
     CDS             complement(4703..>5085)
                     /locus_tag="U4A12_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774899.1"
                     /translation="QLNRIYYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEK
                     ISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIAT
                     LIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1664 a          893 c         1090 g         1438 t
ORIGIN      
        1 ttctttttat tttaataaat accgaactat tttcttattt tttcataaaa tgctcgaatt
       61 tttgaagaaa aacttatata atgtaaaacg ataaaatttc ttaaaaggag ttggaatagg
      121 tgaaagtacg tcgcagtgaa cgtctagttg acatgacaca gtatttattg aatcacccgc
      181 atgaattgat ttccttaaca agctttgccg aacgttatga atctgctaaa tcttctatca
      241 gtgaagattt gggaatcatc aagaaaacat ttaaagaacg agggtatgga accttggaaa
      301 caatcccggg tgctgcaggt ggtgtattgt tcatccctga aatttcatat gaagaggcga
      361 aaaaatatat tgaatctcta gcagaacgtc tatcagaaca agaccgtctg cttccaggag
      421 gatatgttta tctctctgat ttgttaggag aacctgaact gctgcgtcaa gttggaagaa
      481 tcattgcttc taaatacctt ggtgaaaaaa tcgatgctgt aatgactgta gccacaaaag
      541 gcgtacctat tgcccaagct gtgtcctatt atctgaatgc gccattcgtt attgtacgcc
      601 gggactcaaa aataacagaa ggatcaacag taagtgtcaa ctatgtgtct ggatcttctg
      661 aaagaattga aaagatggaa ctgtctaaga gaagtttgaa acgtggttca cgtgtccttg
      721 tagtggatga tttcatgaaa ggcggcggaa cagtcaacgg aatgaagagc ttgattgaag
      781 aattcgaatc agaacttgtt ggcatcactg tatttgccga atcaaaattc aacggtcatc
      841 gcgccatcga tgattatacg tcactgcttt atgtcaaaga tgtagatact caaacgaaaa
      901 atattacggt tgtgccagga aattattttt cagaataaga ggttgtgaaa aagccgctta
      961 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acattttttc ataaatttgg
     1021 cttaataccg agaaactggc ttttgaacac cgtttatcta gaggttgtga aatcagcgtc
     1081 ctgtcctgag tattagctca ataaacggaa aaattgctcc tcatattttg ccgtttattg
     1141 agctaatacc gaaggtcagc tgattgaaaa acgtttaatg aagaggttgt aagcctaccg
     1201 tttaactcta agtcacaaga aacgatttca aaaaacagct tttcatgttt tatgagtagt
     1261 gtgacttgtg accgaggagt tggcaggaga gcaccggtta ccaaatagat aggcccgtta
     1321 tggtctatct atttttttat ccagaaaacg ctgggataaa agaaaatcac ttgttttgtt
     1381 gaaaaaatgg gtatactggg ataaactgaa atcagcaaac tatatttagg agtgacgcag
     1441 ttggacgcac gttatgcaat tattttggcg gcagggaaag gtacccgcat gaagtcaaaa
     1501 ttatataaag ttttacatcc cgtttctggt aaacctatgg tagaacatat cattaatcgg
     1561 gtcagtgaaa cgaaaccaga tgaagtgatt acgatcgttg gacacggagc agaacaagtt
     1621 aaagcacaac taggagaacg cagtaaatat gcgttacaag cagaacaatt gggaacagga
     1681 catgctgttt tgcaagctgc ttctttcttg gaaggaaaaa aaggaacaac gttagttatc
     1741 agtggcgata caccattatt gactactgaa acgctgaaca atctttttga atatcaccaa
     1801 ggaaaaaatg caagtgcaac gatcttgacg gcacaagcag aaaacccaac aggatatgga
     1861 cgaatcatcc gtgaccatat tgggatcgta gaaaaaatcg tggaacaaaa agatgcaaca
     1921 ccagaagaag ctcttgtaca agaaatcaat acaggaactt actgttttga caacgaagct
     1981 ttgttcgatg cgttaagcaa agttggaaca aacaatgctc aaggtgaata ctatctaaca
     2041 gatatcatcg aaatcttaaa agaagaaggc catacagttg ctgcttatca aacggatgat
     2101 tttgaagaat caatgggagt caatgatcgg atcgcgttag ctaaagcaaa tgagatcatg
     2161 agaaagcgaa ttaaccagat gcatatggtc aatggcgtaa gctttgtcga ttcagcaacg
     2221 acatacatcg atgcaggcgt agaaatcggt ccagacacat tgatcgaagc aggtgtacaa
     2281 atccaaggaa atacagtcat tggttctgat tgtgtcatcg ggtcacattc aaaaatcgta
     2341 gacagtagga ttgaggatca cgtcgtcatt gaaaactcag tcattgaaag cagccatgtc
     2401 aagaaacatg cagatgtggg accttatgca catttacgac caaaagctga aattggcgaa
     2461 aacgtccata tcggaaactt tgtggaagtg aaaaatgctc aaatcggcaa aggaactaaa
     2521 gtcggtcact tgacttatgt tggtgatgct acgctgggag aagaaatcaa tgtcggatgt
     2581 ggtgttgtct tcgtcaacta tgatggaaag aacaaacatc atacgactgt cggcgaccac
     2641 agctttattg gttctagcac gaatatcatc ggacctgtcg aggtagctaa aaacagttcg
     2701 attgctgccg ggtcaacaat tacagacaat attccagaat atgcgctggc aattgcgaga
     2761 gcaagacaag tcaataaaga aggatatgca aaaaaactcc cttatctaaa ctaagacttt
     2821 tgcaaaatca cttgatttat ttaaaaaata tgactactat ttattaggta gaattctaga
     2881 gttgtgaaaa agaaagtgga ggtccccatg tcaaaacatt actttgaccc aagattaaaa
     2941 atttttgcgt taaattccaa tcgcccatta gcagagaaaa ttgcagcagc agtcggcgtc
     3001 gagttaggaa aatcatctgt tactcaattt agtgatggag aaattcaagt aaacattgaa
     3061 gaaagtattc gtggatcgca tgtctatgtt attcaatcaa cgagcagccc agtcaatgat
     3121 aacttgatgg agttgctgat catgattgac gcactgaaac gagcaagtgc aaaaacaatc
     3181 aacgtagtca tgccttatta tggctatgcg cgtcaagacc gtaaagcacg tgcacgggaa
     3241 ccaatcacag cgaaattagt agcaaacatg attcaaaaag ctggtgcaac aagaatgctg
     3301 actttggacc ttcatgcagt ccaaatccaa ggtttctttg atattccagt cgatcatttg
     3361 atgggtgcgc cacttattgc caattatttc ttagaaaaag gaatcaaagg cgatgatgtt
     3421 gtcgttgttt caccagacca tggtggtgtc acacgggcac gtaaattagc ggaatttttg
     3481 aaatctccga ttgcgatcat agacaaacgc cgtccaaaag caaatgttgc agaagtaatg
     3541 aatatcattg gacaagtcaa aggcaaaact tgtgtactga tcgatgatat gatcgataca
     3601 gcaggtacga tcacattggc tgccaatgct ttgaaagaag ctggtgcaac tagtgtgtat
     3661 gcttcatgta cacatccagt attatccggc cctgctttac aaagaataga agattcagct
     3721 atcgagcgtc tagttgttac agactcgatc tatcttccag aagatcgtaa aatcgacaaa
     3781 atcgatgaag taagtgttgg cggactaatg ggagatgcaa tcaaacgtat ccatgaaaac
     3841 aaaccagtta gtccattgtt tgaaacaaaa aataaataat aaataaactc acaaagggga
     3901 gtccaattgg gctgagattg aatctatttc taaacccttc gtacctgtat cggttatgcg
     3961 agcgtaggaa ttgtgaataa acagctgttt gactattttc gaaagctgct tcctcctttg
     4021 tgactttttt attcaaagga ggaagttttt ttatgggaaa aaatagagtt tgggtagaag
     4081 gaacgattgt tgcagcgtta gcgatggtgt tgtcgttgat tccgatccag atcggcagca
     4141 gtttttctat ttcattaggg cagattccat tgactctttt tgcgttaaga agagggacaa
     4201 aggcagggat gctggcaggt ttcatttggg gcatcctaca ttttccgttg ggacaagttt
     4261 attatttaag tgtcgttcaa gtgttgattg agtacccgat tgcctatact tttgcaggcg
     4321 cagcagggct catggcttca aatgtccaaa aaggctatgt agacgataat acgaaacaaa
     4381 ttagaaaaag catcgtgttt ggtgcattga ttggcgcact ttccagatat ttttggcatt
     4441 ttattgcagg agtggttttc tggggagcgt atgcactatg ggggatgaat ccttggctct
     4501 tttctttcgt aatgaatggt gccagcgggt tgagtacagc tattgtaacg atcatcgttt
     4561 tattagggat ccaacaaacg actccttcga tttttgtgcc agataaacga caggagctgt
     4621 aaataaaaaa gaagcaagaa acttgagaca gaatcatgtc ctagtttctt gtttcctttt
     4681 tattttttga taagaaatag atctacttca atggcagata agaaagatcg gcatcttcta
     4741 ccaattgcca gtgtcgatcg gtgacagagt agattaaagt agcgatgatt tcctcttctc
     4801 gataccgggt gccttttaat ttccaaatat aatcttcaat cacatagatt gggatacgat
     4861 atttaccatc gatcaaacca acttgatcaa aagaaaaaat gatcccttcc tgcatcaaat
     4921 tacgcatttc tttcaataat tccacactga ttttttcaac aggtgtttct tttttgaaaa
     4981 aagatttttt tacattgatc ttatctgaca agatcgaact taatttacta gcaaaaatcc
     5041 tataaaattc atctaaatag cgataataaa tccgattcag ctgat
//
LOCUS       DASJOX010000154         4952 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJOX010000154 DASJOX010000000
VERSION     DASJOX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.158"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(354..716)
                     /locus_tag="U4A12_002567"
     CDS             complement(354..716)
                     /locus_tag="U4A12_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774900.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(790..1170)
                     /locus_tag="U4A12_002568"
     CDS             complement(790..1170)
                     /locus_tag="U4A12_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN1774901.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1234..2427
                     /locus_tag="U4A12_002569"
     CDS             1234..2427
                     /locus_tag="U4A12_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1774902.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2504..3088)
                     /locus_tag="U4A12_002570"
     CDS             complement(2504..3088)
                     /locus_tag="U4A12_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1774903.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3289..4287
                     /locus_tag="U4A12_002571"
     CDS             3289..4287
                     /locus_tag="U4A12_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1774904.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4399..4908
                     /locus_tag="U4A12_002572"
     CDS             4399..4908
                     /locus_tag="U4A12_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN1774905.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1571 a          925 c          861 g         1595 t
ORIGIN      
        1 ttcgcaagtg gttctttttt aacgactcag ttatcttatc atgtcgttag taacatgtca
       61 ataactattt tttaaatttt ttagaaatgt gatataacat ccacttcaga atcaacgctg
      121 ttaaatttat catgtatttc ttccttggtc aactataaat taagcattac tttgtgaaat
      181 gatttttcga tcaattatta ttattcgtta tacaaccgtt ttcaacatat tttgaaaata
      241 aaatgcacta aaaaactatc tttagcagaa catttcattg tattttttgg accaaacaat
      301 aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt attttagaag
      361 aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc tggttcttcc
      421 caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc aaagacaaac
      481 aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact aatcttgcta
      541 acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc agtctttcct
      601 tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac caaaaatttt
      661 ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct tgccaaaatt
      721 tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt ttcagaaaaa
      781 atacactttt tatttcttat tttctttccg ttctagttca gataatgctt cttctatcca
      841 aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat tcgtgaaata
      901 cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta aagataaact
      961 gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct gtccgacttt
     1021 taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat gaataagtcc
     1081 ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg tcacttttcc
     1141 ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat ttatcattat
     1201 actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa
     1261 tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc aattgctgag
     1321 acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt cttgtctccc
     1381 ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg ttatgccact
     1441 ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca tgctgtggag
     1501 ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta
     1561 caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta tcctccattt
     1621 tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat
     1681 aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa
     1741 gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa agaagaattg
     1801 aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga cgagatccat
     1861 caagatttaa ctttattcga tcatacattt acttccatgc taacgattga tcctaattta
     1921 gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc tgcaatcaaa
     1981 aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa acatttagtg
     2041 atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc ggcttatgag
     2101 acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt
     2161 aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga aggaacttac
     2221 ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga gaacactctt
     2281 gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc cggtcatggg
     2341 catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact taatcgaatc
     2401 aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga cgagacctta
     2461 ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct gaaatttcaa
     2521 ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt acatttgcga
     2581 tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt cggaaatcta
     2641 gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat ccagcacctt
     2701 ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag aattggcttc
     2761 cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg aacagttcac
     2821 gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt cctgttccag
     2881 ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc acgccatcat
     2941 aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct agttcaggga
     3001 ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt cctttttcac
     3061 tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc atacttcata
     3121 ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt cttttatttt
     3181 tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac atttttgaga
     3241 agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat ggaaatctat
     3301 gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta cgcaggaatg
     3361 cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca attagcaact
     3421 ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa agcaagtgaa
     3481 ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca tttaaaagaa
     3541 gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa taaaggcatc
     3601 cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc aagaaaattg
     3661 aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt gaatgatttg
     3721 atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc tatcgattgg
     3781 acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt
     3841 cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt gcttactccc
     3901 tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg gttaaaaaaa
     3961 gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa ttatggattc
     4021 tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa tgccattact
     4081 gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga catctgtacc
     4141 tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac tgcagtgaac
     4201 aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag cacaagctta
     4261 tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc aagttaaacc
     4321 cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa
     4381 taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa
     4441 ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat gtttttacaa
     4501 aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa cagcgtgcta
     4561 acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat attagcagaa
     4621 aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg cctttacggt
     4681 atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat
     4741 tatggttata tcgacaaggt gaaacctggt attttaaaag aactaaacag ccacgatggc
     4801 gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc tgctgccagt
     4861 cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa
     4921 agaaagaaaa cgtctattta gacgagttct tt
//
LOCUS       DASJOX010000155         4783 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJOX010000155 DASJOX010000000
VERSION     DASJOX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.159"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..1234
                     /locus_tag="U4A12_002573"
     CDS             44..1234
                     /locus_tag="U4A12_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEN1774906.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1368..3557
                     /locus_tag="U4A12_002574"
     CDS             1368..3557
                     /locus_tag="U4A12_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1774907.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3554..>4783
                     /locus_tag="U4A12_002575"
     CDS             3554..>4783
                     /locus_tag="U4A12_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1774908.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1720 a          735 c          863 g         1465 t
ORIGIN      
        1 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
       61 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
      121 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
      181 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
      241 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
      301 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
      361 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
      421 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
      481 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
      541 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
      601 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
      661 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
      721 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
      781 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
      841 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
      901 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
      961 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
     1021 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
     1081 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
     1141 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
     1201 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
     1261 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
     1321 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
     1381 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
     1441 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
     1501 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
     1561 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
     1621 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
     1681 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
     1741 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
     1801 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
     1861 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
     1921 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
     1981 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
     2041 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
     2101 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
     2161 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
     2221 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
     2281 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
     2341 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
     2401 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
     2461 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
     2521 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
     2581 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
     2641 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
     2701 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
     2761 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
     2821 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
     2881 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
     2941 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
     3001 tatcctccct ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg
     3061 aaattgagtt tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag
     3121 atgacgcatt caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac
     3181 aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt
     3241 ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta
     3301 atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac
     3361 tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa
     3421 aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag tactttggta
     3481 aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg
     3541 ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca
     3601 aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa
     3661 aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa
     3721 aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag
     3781 tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct
     3841 tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat
     3901 tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa
     3961 tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa
     4021 tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga
     4081 aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa acagtaacgt
     4141 tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc aagatactag
     4201 agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat
     4261 gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag ctaaatcagt
     4321 tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac atggctgtta
     4381 caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc cttacgagat
     4441 tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt gcaaagttac
     4501 taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg aaagcggcat
     4561 tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt cactgggtga
     4621 gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat caccaattaa
     4681 ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg aatcaggtga
     4741 aaaggttcga agaagaaagc taattgcaaa cgatatttta att
//
LOCUS       DASJOX010000156         4775 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJOX010000156 DASJOX010000000
VERSION     DASJOX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.160"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1128)
                     /locus_tag="U4A12_002576"
     CDS             complement(<1..1128)
                     /locus_tag="U4A12_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1774909.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="U4A12_002577"
     CDS             complement(1164..1874)
                     /locus_tag="U4A12_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1774910.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="U4A12_002578"
     CDS             complement(1942..2757)
                     /locus_tag="U4A12_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN1774911.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="U4A12_002579"
     CDS             complement(2768..3736)
                     /locus_tag="U4A12_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1774912.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>4775
                     /locus_tag="U4A12_002580"
     CDS             4499..>4775
                     /locus_tag="U4A12_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1774913.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT         1473 a          983 c          726 g         1593 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagt
//
LOCUS       DASJOX010000157         4768 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJOX010000157 DASJOX010000000
VERSION     DASJOX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.161"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(182..1144)
                     /locus_tag="U4A12_002581"
     CDS             complement(182..1144)
                     /locus_tag="U4A12_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1774914.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="U4A12_002582"
     CDS             complement(1146..2585)
                     /locus_tag="U4A12_002582"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN1774915.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="U4A12_002583"
     CDS             2770..4716
                     /locus_tag="U4A12_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN1774916.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1520 a          814 c          785 g         1649 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatata
//
LOCUS       DASJOX010000158         4720 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJOX010000158 DASJOX010000000
VERSION     DASJOX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.162"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1097)
                     /locus_tag="U4A12_002584"
     CDS             complement(<1..1097)
                     /locus_tag="U4A12_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1774917.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="U4A12_002585"
     CDS             complement(1227..2603)
                     /locus_tag="U4A12_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774918.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="U4A12_002586"
     CDS             complement(2603..3259)
                     /locus_tag="U4A12_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1774919.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="U4A12_002587"
     CDS             complement(3269..4546)
                     /locus_tag="U4A12_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1774920.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="U4A12_002588"
     CDS             complement(4565..>4720)
                     /locus_tag="U4A12_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN1774921.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DASJOX010000159         4709 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJOX010000159 DASJOX010000000
VERSION     DASJOX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.163"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            106..357
                     /locus_tag="U4A12_002589"
                     /pseudo
     CDS             106..357
                     /locus_tag="U4A12_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="U4A12_002590"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="U4A12_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1774922.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="U4A12_002591"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="U4A12_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="U4A12_002592"
     CDS             1786..1869
                     /locus_tag="U4A12_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEN1774923.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="U4A12_002593"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="U4A12_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1774924.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="U4A12_002594"
     CDS             complement(2994..3857)
                     /locus_tag="U4A12_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEN1774925.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="U4A12_002595"
     CDS             complement(3859..4131)
                     /locus_tag="U4A12_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1774926.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="U4A12_002596"
     CDS             complement(4148..4357)
                     /locus_tag="U4A12_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1774927.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4709)
                     /locus_tag="U4A12_002597"
     CDS             complement(4455..>4709)
                     /locus_tag="U4A12_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEN1774928.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          795 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgat
//
LOCUS       DASJOX010000160         4598 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJOX010000160 DASJOX010000000
VERSION     DASJOX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.164"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="U4A12_002598"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="U4A12_002598"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN1774929.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="U4A12_002599"
     CDS             1085..2005
                     /locus_tag="U4A12_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1774930.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="U4A12_002600"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="U4A12_002600"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN1774931.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..>4598
                     /locus_tag="U4A12_002601"
     CDS             3311..>4598
                     /locus_tag="U4A12_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774932.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGF"
BASE COUNT         1607 a          746 c          897 g         1348 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggattta
//
LOCUS       DASJOX010000161         4519 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJOX010000161 DASJOX010000000
VERSION     DASJOX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.165"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(85..339)
                     /locus_tag="U4A12_002602"
     CDS             complement(85..339)
                     /locus_tag="U4A12_002602"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1774933.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="U4A12_002603"
     CDS             complement(450..704)
                     /locus_tag="U4A12_002603"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1774934.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="U4A12_002604"
     CDS             complement(897..1172)
                     /locus_tag="U4A12_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN1774935.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="U4A12_002605"
     CDS             complement(1144..2097)
                     /locus_tag="U4A12_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1774936.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="U4A12_002606"
     CDS             2709..4202
                     /locus_tag="U4A12_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1774937.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4519
                     /locus_tag="U4A12_002607"
     CDS             4337..>4519
                     /locus_tag="U4A12_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN1774938.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSL"
BASE COUNT         1464 a          767 c          775 g         1513 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtcctta
//
LOCUS       DASJOX010000162         4511 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJOX010000162 DASJOX010000000
VERSION     DASJOX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.166"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..974)
                     /gene="lacD"
                     /locus_tag="U4A12_002608"
     CDS             complement(<1..974)
                     /gene="lacD"
                     /locus_tag="U4A12_002608"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1774939.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(971..1429)
                     /locus_tag="U4A12_002609"
     CDS             complement(971..1429)
                     /locus_tag="U4A12_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1774940.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1426..2889)
                     /locus_tag="U4A12_002610"
     CDS             complement(1426..2889)
                     /locus_tag="U4A12_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1774941.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2871..3803)
                     /gene="pfkB"
                     /locus_tag="U4A12_002611"
     CDS             complement(2871..3803)
                     /gene="pfkB"
                     /locus_tag="U4A12_002611"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1774942.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3945..>4511
                     /locus_tag="U4A12_002612"
     CDS             3945..>4511
                     /locus_tag="U4A12_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1774943.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGDKTNKNIKNKIYVFIFQ"
BASE COUNT         1351 a         1017 c          723 g         1420 t
ORIGIN      
        1 tacttgttaa cataaataga agttgctgtt tcttctaata gtgtacttaa ttctttaata
       61 ttttgttttc cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa
      121 ggtgctactc ccttcgccca tgttgctcgc ccacataaga caccattgaa tgtcgaacct
      181 gattgttttg caaaacgcaa tgtatcttga aatagtgcag cgctgactcc cgcacttaaa
      241 aaaataaacg gttgttcagt cactttcgat tgttcataga aatacgcgga tgcctctgct
      301 tgactataac aagtatcttg cccatatcct tccacaaatc tcatattcac tggtacttct
      361 actttcaata catccacttg ataacgttct tttgaaaatt ctcgcatggc ttcaatcact
      421 ttatgcggtt ttacttttgc atagtcaaga gaagacggat cgccaatagt tgcatcgtaa
      481 gaaaggagct ctaaaaagaa aggtatgcct tctgcttcgc attctgaacc aatccgttct
      541 ataactattt gtttttgcac attaatctct actggttcat caacgtcata gtacaataaa
      601 aatttacaag catccgcacc cacttctttt aaccttttca ctgaccaact attcagtaaa
      661 tctggtagtc ggcctggcat tttcgcatca tagccggttt tttcataagc tagcaacaaa
      721 ccacattctt tctctcgaag cgctgctgca ggcagaccat attctggatc gagtaaaata
      781 gcggaagcat acggagtcag gtactgagaa accatctgtt taaattggat gatatcttct
      841 gatgtagctt ctttatattg gttgatcatt ttcttcaaag caccacgttg gtcgatagct
      901 agcgcgctaa taattccagc ttcatccgtc aatcgcttca aacgttcctg tttttctata
      961 ctcattttct tcatatatct tcacctattt ctttagttaa tattgtagct atttctattt
     1021 ttgattttgc agctctcaac tgttctgtaa cctcttttcg catcaatgat cgtgcaacga
     1081 gagacaataa tttcaaatga gtcgttcctg cttctgaatc tgggataaat aacgcaaaaa
     1141 taaaatcaac tgactgtcta tctaaactgt tccattcaat tccttttttc agtgtgacga
     1201 taatcaaact aggttcattg attgtttgag acttcgcatg agggatagca aaacctgaca
     1261 ttattcctgt agtaccttct ttttctcttt ccagaagttt ttgatatact tctttttcgt
     1321 catgagcgat tcctaaacag aatgtttgct ttgctaaaaa cttaaatgct tcttcttgat
     1381 tatctaatgg ctgttgtaaa aaaatgttct cttcatgaat gattgtcatg ataaacctcc
     1441 tgctcttcct tcttcgataa ctgtacgatt tgttgcttta ttatccctct tcttgtcttt
     1501 atcagttact cttttctttt ggatcaaacc tagtatcact ccaccaacga tcgaaccaat
     1561 aagaatcgct gtaacccatt ggatttttcc cgtcactaca ggtaacacta aaaaaccgcc
     1621 atgtggggct ggtacttgga caccaaacag ataggtcaag acagcagcaa ttgatgagcc
     1681 taacatcatc gttggtaata ctcggatcgg gtctttcgca gcaaacggaa tagctccttc
     1741 cgtaatatga gttgatccta aaataaaatt aaccaaacct gcattacgat cattagtatc
     1801 aaaatattta ccaaacaata aagtagcaaa accagtaatc aacggtggtg caatgcaagc
     1861 tgctgacgca ccagccataa aagtcgtgtt tccttgtgtc aaaagagctg ttcctgttac
     1921 atatgccgcc ttattaaccg gtcctcccat atcaaacgca cacatacagc caacaatcac
     1981 gccaagaaca agtggattcg aatattcaaa tccagctaaa aagctcatca ttccttcatt
     2041 tatagccttc attggacctg ccagcaaaga catcacgatg ccaataaatg ctacaccaat
     2101 aactggatat aagacgattg ccttcaagcc atctagttgt ttaggtaaaa ttatcagtag
     2161 cttttgcaaa aataagataa aataacctgc taagaaccca gctaaaatac ctcctaaaaa
     2221 tccagtaccg ccattcatgg ctacgattcc accaacaaat ccaacgacta accctggacg
     2281 tttcgcgatc ccttctgcaa tataagctgc taaaactgga accatcattc ccattgctgc
     2341 atcgtcaact gcttttacca ttgccgctgt agcattgtat tgtgcatgat ctggatcaga
     2401 agaatagatt ccccaaacgg caaatgaaat agcaatcaat actccgccag ctaccacgaa
     2461 aggcaacata tgagagacac cgttcatcaa atttttatag atgctacgac caaaattagc
     2521 accacttatc gcttctggtt ctaaagtatc agcagcttca ttatctgcta agacagtacc
     2581 ttttcctgcc aatgcttcgt caatcagccg ttgtgcatcc ttgattcctg tactgacaga
     2641 aacatcaatg actcgttttc ccacaaatct ttctgaatgg acatctttgt ctgcggcaat
     2701 aatgacagca tctgcctgtt tgatttcttc agccgttaat tcattctcga tcccaatttg
     2761 tccatgggtc tccactttga tcgtgatgcc ttttttccta gctgcttgct ctaatgcctc
     2821 ttgcgccatg taggtgtgcg caattcctgt cggacaaccg gttgctgcaa ttagttgata
     2881 ttttcccatc tttcgaacct ctcttccttg attttgattt gtttacttaa ttcctccaca
     2941 tccaaaaaat cagtcagtcc tttacgaaaa gcagtagagc tgcctgctgc tatgctttta
     3001 cgtaacgttt catcaagcga tcgtcgattc atgtatcctg ctaaaaaagt gcctaacatg
     3061 gcatcacctg cacatgctgt attgacaact tttccttttg gcgagtttcc tcgaatcacc
     3121 ctgtctttcg taaaaagaat cgctcctttc tcaccgaggg ataacaaaac attttctgcg
     3181 cctgactcta ctagtcgttg tccataaaca aagtaatctt catctgtaac catttttcgt
     3241 ccgaaccaag aagaaagttc ttcctcattt ggtttcagca aaaacggtcg atagggtaaa
     3301 caatccatga cttcttgatc actactatca ataatcaaac aaatccgact gatttctatt
     3361 aaaacctttg gagacagccc tcttggtaaa ctcccagaaa cacaaagata atcccctgct
     3421 tgtaatgctc ttatttgatt taaaaaatta tgaacagctg tctgcggtat ttcaggtcct
     3481 tgattcacta atttgtattc ttctcccgtt tgattgactt gtgtaaatac attgatacgc
     3541 gtcataccag gcacttcaat aaactctgtt tggatttgtt tctgctgtaa atagtcttct
     3601 atatactttc ctgtgaaccc agcagaaaaa cctaatgccg tacttgggaa atctagcatc
     3661 tttaatacta gtgacacatt cacccctttt ccgtttgcct gaatgtcatc ctcttttgtg
     3721 cggtttacca taaaaggatg catctcatct gtttcaataa atagatcaat tgccaaattc
     3781 atcgtgcatg tgtagataga cataaccaac gacctccttg attctattat gaaatacgcg
     3841 aaatagaaaa tgttttctta atcgtcccaa cacaaagaaa acctttacat ttataaactc
     3901 gtcgtgcttt ttttaactaa cttttggtga aagaaggaaa gaatatgaat cacaaattaa
     3961 gtgaaacaga gcagtacctc tggaacttta tcgaacacct catcctagag atccctaact
     4021 attccatcgt gaaactaagt gaacaagcaa atgtctcaac agccaccatt gtgcggacaa
     4081 tgaaaaagaa aggctatgaa gggtttactt cgtttaagca tcatctaaaa gaaggagaaa
     4141 acaagaacat caatttttca tttttagata aagtagacaa aggaatccgc cgagccattc
     4201 taaaaaatga acaagaagtg gtacgtacaa tcaatatgtt ggaaatcggg aacattgaag
     4261 atgcaataca aagaatcaaa tttgctgacc gggtttttgt ctttgcccga ggattttcag
     4321 aaatgattgg ccaagaaatg ctggtaaaac ttcaattaac gggaaaaaat tgtcagatgc
     4381 atactgatcc agaaattatc aaaagcatca gtcggaaact aactaagaaa gacagcgtta
     4441 tttttgtttc tttgaacggt gacaaaacaa ataaaaacat caaaaataag atttatgtct
     4501 ttatttttca a
//
LOCUS       DASJOX010000163         4507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJOX010000163 DASJOX010000000
VERSION     DASJOX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.167"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            547..1458
                     /locus_tag="U4A12_002613"
     CDS             547..1458
                     /locus_tag="U4A12_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEN1774944.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1945..2211
                     /locus_tag="U4A12_002614"
     CDS             1945..2211
                     /locus_tag="U4A12_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774945.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2283..2624
                     /locus_tag="U4A12_002615"
     CDS             2283..2624
                     /locus_tag="U4A12_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774946.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2679..3098
                     /gene="ssb"
                     /locus_tag="U4A12_002616"
     CDS             2679..3098
                     /gene="ssb"
                     /locus_tag="U4A12_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1774947.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3175..3471
                     /locus_tag="U4A12_002617"
     CDS             3175..3471
                     /locus_tag="U4A12_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774948.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3486..3917
                     /locus_tag="U4A12_002618"
     CDS             3486..3917
                     /locus_tag="U4A12_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1774949.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4099..4404
                     /locus_tag="U4A12_002619"
     CDS             4099..4404
                     /locus_tag="U4A12_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774950.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1658 a          707 c          959 g         1183 t
ORIGIN      
        1 cttatgagag aaaatggtca aaaacctaag cgggaagtct ccgaataaca taccaagcgc
       61 cgacagccaa cagataaaga atacactact aaaaatagta gtagcataaa atttaaaagg
      121 gaaaaaatca acccctaccc cagtcttggg gtctcaaaac taactccccc ctaaccccct
      181 ctttcagcct cttttgaaag agggggagaa tcaaccccac ggccaacgcc ccagtgttct
      241 tggggctgtc cagaacagaa acttgcgaga gagtgggggt aaaacaagtg tagaggaaac
      301 ttgaagactt gttcgaacag aaacttgcga gagagccgga gtaaaacaag tgtagaggaa
      361 acttgaagac ttgttcgaac agaaacttgc gagagagttg gagtaaaaca agtgtagagg
      421 aaacttgaag acttgttcga acacaaactt acaagagagg ggagtaaagc agttgcagag
      481 gaatcaggaa gatttgttcg aacagaaact tgcaagaaag cattattaaa ttgaaggtga
      541 tgaaatatga agtatttatt gcatggctat tttattgatt attctactcg caatagtagt
      601 actttagcga ttctatgttt gcttcatgaa ttgagagtgg ttactagaaa acagctatac
      661 aaactattga attttgacag atcaattagt aaacaaggag taacgaatgc tttagtgaga
      721 ttatatgaaa aacagttaat cgatcgaaaa aaatatggaa cagaaatgta ttattatttg
      781 acgaaagagg gacatcaaag cataggtggg tattatacgt tgccgaaagt tcctgaatat
      841 aatttgaatc accatttaca aattaacaat tatttgatta aaggaatggc attggcaaaa
      901 gaccacccac atttaaaacg gattatttca gaaagacgta ccgtctatga aacgaaggat
      961 ttaaatccta agacaaaagg aagaaaattt tttgtgccag attttatttt tcgttttcga
     1021 gacaaagaaa atcatgagat caattggtcc tttgaaattg agctaacgtt gaaaacacgt
     1081 cgtcgctacc gtgaagcgat ttttccgaaa tatattcgtg agttgcgaac acgtcctgaa
     1141 tcacatttaa tttatgtgac acctagtggc gcaattcaag aagaacttca tgcgtttaag
     1201 cgtatttttt tcaagcgcga aactgaaaaa gatgcgagtc aacaagaatt gtttgagcgg
     1261 ttgcatattt tttcagcttt agagtttgaa gagggattaa aagcattatt agagaatgat
     1321 ccagcagtca attggcggac agtagaacga gaaaaaattt atccgaatgg cgctgttcaa
     1381 agtcgtttga atttatttga agaatcaact gaaaatgagg aaaaaccaaa agtgaagttt
     1441 acgcctgaag tattgtaaaa gagggtgttc gcttcgctct ttttttgtgc cagtttgctg
     1501 gcaccgttca gacgcaagcg tctgaaccgg ctcgttttta ggaaggacta acttgtttga
     1561 tttatccgtt gtcaaagaag tggacgttcg agagtcccta atcgtatagc agtcattcgg
     1621 cgagtataac ctaaaacata acctgccaat tgccaagggt aaaatgcacg ctgcgcgccc
     1681 ttggcaatcg tcagaacatg ttttcgagcc ggttatgccg aacgaccgct atacgaaaag
     1741 ggcctctaaa cagaagtcca ctttgatcct tgacaactga aacccaaaat caaacaagca
     1801 ggccttcgtc taaaaacgag ccgaatgaag accttggggg ttgcaccccc aaacccccgt
     1861 gcccccgaca cactaccgaa aaggtggggg tgggtggcga aaaaatcaaa taaaacattc
     1921 tattcaagaa aggaagaaac aaccatgaca actaaaaacg agcaactgaa agaacaggca
     1981 agaaaagagt tgcaagaaag aggtttgatt attgagggga gctttgaagg agattttgaa
     2041 acctatattg gttgttatgc aagaccgata aacaaaccta cagccttaga cccaatgaac
     2101 gaacaagaag taaaagaaca agaaaaacat gcagtgaatg gttttccgca agatttcact
     2161 gaatggtatg agtgggaaat taacaacggg aagcttgaaa acttcttata aaagtcagat
     2221 gacgtgcaaa gagaatgatt ctctttgtac ggtaaaaata taaagaaaga aggaaaaaga
     2281 aaatggcaaa aaacacacct tatcaagtag gagacatttt ttatagtagt tggggctacg
     2341 aacaaacgaa cgtgactttt tggcaaattg tgaagttaac agaaaaaaca gcatggtttc
     2401 agcctattca aaagaaaacg gtcgagcatt attcgtccat gagtggaaaa gttgttccaa
     2461 tgaaagacaa gtttatagag tatcctttag caaaaactaa agacagtatt gtaaaaaaac
     2521 gaattgatcc agaaacctat cctgatatgt tatgtggtta tcgtagttgg gacacgtttc
     2581 atagatataa aaatcaagct gtttatgaaa gtagttatta ttaaggggat agtttcccct
     2641 ttcaaaaata ataaatagaa gaatggagag aaaaaatcat gatgaattca gtgaatttag
     2701 taggacggtt aacaaaagaa gtggatttaa aatacacacc aaaaggcaat gcgacaggca
     2761 catttatttt agctgttaat cgaaattata cgaatgcaaa cggcgagcgt gaagcagatt
     2821 atatccgctg tgtcatttgg cggaaagctg cggaaacttt agcaaaatat actggaaaag
     2881 gaagtttgat tggcattaat ggacgtcttc aaacgaggag ctatcagaat cagcaaggac
     2941 agaccatgta tgttacggaa gttctagtga cagatttcta tttgttagag agtaaagaaa
     3001 tcaatgagca acggtcaaaa tcaatggcga ataatcaaaa taattcttca attccaggaa
     3061 atgagattca aatttcagat agttatttgc ccttttaagt gaaaaataat tttgttctat
     3121 acctgatttc ttcaggtata gaacaaaaaa taaatagaga tgaggcgatt ttttatgaaa
     3181 atcattgaca gttacgatca acgaaccatg caatatttgg gctcgtttga ttatacagat
     3241 gaaaatatta ttaattatgt tgcaagttta cctccttatc aaagcgttcg cttggtagat
     3301 tttgcaagtg atgaattggt attaacaacg atgggcaact ttttagatta tgtcggagac
     3361 aaaaaatggt tggaaaaaat tttgtctaaa ttgattgcga aacaaacagg agaagaaccc
     3421 ataaatgcga ttcaattgtt caatcgatat gaacaagaaa acaatgatta aagaggagag
     3481 aaaaaatgaa aagattaaaa cgaagtattt ttattgtgct agcattgatt gattttgttg
     3541 ggacaggttg tgaagcaatt gacgttcaaa aagctccctt atcggaaata aaacaagcaa
     3601 taacaaaaca agatgaagaa caaccgctaa atacacaagt agaaatagtg aatcaaaata
     3661 aaccgagttt ttctgaagaa gatttttcgt tagaaaatgg aaccaggcaa tttttttcag
     3721 atttggatcg tttaaatcgg gtaggtgtag caagtgcact tttgcataaa agcaggatgc
     3781 caacagaaga acgagaccga ttatacatta agccaactgg ctggaaacaa aagaaaatga
     3841 agaatggcga ttatctttat aatcgttgtc atttgattgg ctatcaacgt gcgacaagtt
     3901 tccccataag tatgtaatac gaaacgggag gattaatctt aagattaatc acaactaacc
     3961 tatttgacag ttggaatgaa agctatcatg tctgctgaat caactggtct gatactccta
     4021 catgcaataa agtctactag gaagttattg tatgaagcta ggtaaaggcg gcagaacaat
     4081 ttctaagtgt ggaatcatat gaagttggat atgccgggac gctataaatt actcagggtg
     4141 agactgtttt taattaaact gacgaacttg cgaatgtacg ggtctataac cgacttatat
     4201 tcgaaagata taagattgcg aatgcaaaga acattatcga aaagtaagaa tgttggtcat
     4261 gaaattcgag atggaagact agcggtgtat ctgtcaagag ttggtaaaac atgtttgatt
     4321 gattcagatg agagtgggaa tgcgacaaaa cgttttacag aagaaataga gcaactagca
     4381 gaactgaata tacgatattt ttaaatactt tagcggagaa taaaaaaatc ctctacagaa
     4441 aactacggag ggcaacatgt gctattgttt caacaataca tttttaattt ttaccataac
     4501 aaaaaag
//
LOCUS       DASJOX010000164         4416 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJOX010000164 DASJOX010000000
VERSION     DASJOX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.168"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            264..995
                     /locus_tag="U4A12_002620"
     CDS             264..995
                     /locus_tag="U4A12_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1774951.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1140..3167
                     /locus_tag="U4A12_002621"
     CDS             1140..3167
                     /locus_tag="U4A12_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1774952.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3275..4372
                     /locus_tag="U4A12_002622"
     CDS             3275..4372
                     /locus_tag="U4A12_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1774953.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1548 a          714 c          918 g         1236 t
ORIGIN      
        1 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
       61 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
      121 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
      181 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
      241 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
      301 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
      361 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
      421 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
      481 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
      541 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
      601 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
      661 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
      721 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
      781 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
      841 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
      901 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
      961 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
     1021 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
     1081 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
     1141 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
     1201 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
     1261 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
     1321 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
     1381 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
     1441 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
     1501 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
     1561 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
     1621 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
     1681 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
     1741 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
     1801 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
     1861 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
     1921 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
     1981 agcaaagaag tcttacttat gatttggaag gtgtcggtca ctcttctcgg attatacctg
     2041 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
     2101 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
     2161 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
     2221 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
     2281 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
     2341 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
     2401 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
     2461 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
     2521 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
     2581 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
     2641 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
     2701 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
     2761 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
     2821 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
     2881 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
     2941 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
     3001 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
     3061 ctacagctca tcgtggaatc agaatagata ttcctgtaga aaaccaaaga atcggaacct
     3121 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
     3181 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
     3241 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
     3301 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
     3361 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
     3421 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
     3481 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
     3541 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
     3601 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
     3661 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
     3721 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
     3781 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
     3841 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
     3901 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
     3961 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
     4021 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
     4081 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
     4141 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
     4201 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
     4261 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
     4321 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
     4381 taacaaatag taaattttta ttattgtagg atggtt
//
LOCUS       DASJOX010000165         4397 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJOX010000165 DASJOX010000000
VERSION     DASJOX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.169"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..672
                     /locus_tag="U4A12_002623"
     CDS             <1..672
                     /locus_tag="U4A12_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1774954.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
     gene            1423..2400
                     /locus_tag="U4A12_002624"
     CDS             1423..2400
                     /locus_tag="U4A12_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1774955.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            2410..2907
                     /locus_tag="U4A12_002625"
     CDS             2410..2907
                     /locus_tag="U4A12_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1774956.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            3283..3564
                     /locus_tag="U4A12_002626"
     CDS             3283..3564
                     /locus_tag="U4A12_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774957.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            3566..3982
                     /locus_tag="U4A12_002627"
     CDS             3566..3982
                     /locus_tag="U4A12_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774958.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1655 a          744 c          748 g         1250 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt agctaagtgg tgagtagttc acaacgcctt
      841 agatctaatt ttagaggtct tatagaacat tttagggttg ttagggcttg ccctgaccgc
      901 ctgtaaggcg ctttgagaac aagataaggg ttttatacta agactgtctt aaaacggctt
      961 aaacaaacgc tcagaacttt tcaaaggagt tctttatgaa ctgtttttaa gcaaaggagc
     1021 ctttaggcga ctgcgcagta taatgtgaga gcgtagcgat cacacatttt tagtgaccgt
     1081 agggagcgag tttcccagtt tagaaaaatt cgcgaacttg tttgaggaaa aaagcaggag
     1141 ttttttaggc gggaaatttt gcctcctctt ttataatctc tttttaaacc tctttttaaa
     1201 cctctttacc gtcggctgta tgcttactct cccaaggtgt gcagaatctt ttgggggtct
     1261 ttttgtctgt ttttaggggt ctttttgtct gtttttaggg gtctttttgt ctgtttttgg
     1321 gggtcttttt gtctgttttt aggggtcttt aattaggggc ttgaccccta ttagaaagct
     1381 ctttataatt ttgttaagta gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg
     1441 aaataccacc atgaattaaa tacgattcct ttacgcaaat ttacgtcagt agaaatgaat
     1501 ttattcttct ctatagtatc tagaatgcgt gacgtaggcg ataaaaaagt acaatttaca
     1561 ttcgaacaat taaaagattt aagcaattat aaagcaactg caaatgttcg attcatagat
     1621 gatttagaaa caacgtatga caaattaatg gatttacgtt ttggtagaag aagtgctgac
     1681 ggtttgcaac gtgaacgatt tgttttattt aatcaattta aaatcgatgg aaaagctgat
     1741 attccttttg ctgaaataca agtccatgag aaagccttac ccttacttaa taatcttgaa
     1801 gaatgggttc gatattctct ccaacaattc aatgaattag aaagcagtta ttccaaaacc
     1861 atgtttagat tattaaaaca atacagaacc aaaggaattg cctatttttc aaaagaagac
     1921 tttcatgaat tacttgatat ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt
     1981 ataaagccta ttcgccaaga actaaccgca atcttcaaag gtctgacaat caaaaagaaa
     2041 tacggaaaag gaagaggaaa acctgtcatt ggctatcaat tcacgtttaa acctgaaata
     2101 aaaaaagcag acgactttaa taaacacgca ccaacaaaag aagaacaaaa taaagcttta
     2161 gaactagtta atcaacaaat ttactctgaa aaagacccca cttcattaca agaaaaaatc
     2221 aaagaacaga aacgaaaatt agaactttta caacaattag aagagctaga aagaaaacaa
     2281 aataatctga ataaagaaaa taaggagatt aacgaacaaa taccagataa caattccgat
     2341 attcccccag aattaaaaaa aggcttacta gataatcttc aaaacttatt taaagaataa
     2401 ggggttttta tgacagataa aactatcaaa gaattagcag atattctagg tgtttcaaaa
     2461 gataaagtga agtatcaact gaggaaatta cctagtaatt ttacctacaa aaaaggaaat
     2521 attatctacc tgaaaaaaga agcagtaaca acaattacaa aggttttatc aggtaatgag
     2581 ccgggtaact tacctagtga attacctgat tcattaccta ccgattacct actcaaggag
     2641 attgaaacta agaataatca tattgaagaa aaagataggc aaattgaaaa acttcaacta
     2701 gctttagaaa cacaacaaaa attactcgac cagcaacaac aactcaccct gcaagccaac
     2761 caacaaatcg agaaactgca ggaacaacta cagttgactt atacggaaga atcttcggaa
     2821 aataaatcaa cgactctttc tgaagaaaca gaatcgatag aaaaacaatc aaagacggag
     2881 aaaaaatggt ggcaattctg gcaataaaac cagcaattta aattaataaa aattgctggt
     2941 tttattcttc tttttgcttt tgcgagcaga gcaacatggg cccatgttgc gatattttgg
     3001 tggcgcccca atccccgttt tttcgttgaa tcttactcgg actgcactcg ttttacagct
     3061 gatttttctg aattttgaaa gagcataagg aaacgtggta tcactttctt acgaaaggtg
     3121 ctagataagc tgtgcctatc acgtttctca gaaaaaaatt tgctatcact tttttgccat
     3181 tgtggctagg ctatgtatag tgttttggcg aaaaatagct atacaaatac tatatttgta
     3241 tgatatacat aaattatata ctgatacgaa aggaaaattt ccatggacat tttagttcga
     3301 aatatcaatc ccaaaattat tacaagaata gataaacgtt gtcaggaact aacaaccaaa
     3361 acgggcaaaa aatggagtcg aaatcaatac ctaaaaattt taatcgaaaa cgattttgac
     3421 cacgaactta tgaattacaa aaaagatcaa ttcgatttat tattcgaaaa atttattgcc
     3481 attcaagcag aaaatacgaa catactaaat gactatgttc acacaaacaa ccaactcatt
     3541 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgtaaccct
     3601 ttatcccagt cacgttgacg ttttagattc catcatcgaa aaaaatccag acgtcaacaa
     3661 ttattcagaa gccattcgct ttttatgtat gagttatgaa cctgctacca atacaaccac
     3721 aaccagtaac caagcaaaac tcaatgccat gggaaaagaa atttctatca tgacagaaat
     3781 catggcagaa tttgctgatt tatatctaaa agatactggt gttcttatcg gtacaaattc
     3841 taaagtatat caagaagtga aagaaaaaat tgaaaatcaa ctcaatcctt cccgacgaaa
     3901 tcaacccaaa aatcgtcaaa aagaaaccaa tcaaaccttt acttctggaa attttgaccc
     3961 aacaaacttt ctaaaaagat aaaaaaagaa agcgaaaaat aaaaaaattc ctcgatttct
     4021 ttttagggat cggtcttcca gcaagatttg caagttggta ccaacttgcg aaaaaaaaaa
     4081 gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc taatccctaa
     4141 gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg aataaaaaaa
     4201 aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc ccttactgag
     4261 aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa aaagtgaccg
     4321 gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat tagaagctag
     4381 taactagcta gaccaat
//
LOCUS       DASJOX010000166         4348 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJOX010000166 DASJOX010000000
VERSION     DASJOX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.170"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(387..1853)
                     /locus_tag="U4A12_002628"
     CDS             complement(387..1853)
                     /locus_tag="U4A12_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1774959.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="U4A12_002629"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="U4A12_002629"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1774960.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DASJOX010000167         4321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJOX010000167 DASJOX010000000
VERSION     DASJOX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.171"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(851..1807)
                     /locus_tag="U4A12_002630"
     CDS             complement(851..1807)
                     /locus_tag="U4A12_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774961.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1916..2161)
                     /locus_tag="U4A12_002631"
                     /pseudo
     CDS             complement(1916..2161)
                     /locus_tag="U4A12_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2306..2728)
                     /locus_tag="U4A12_002632"
     CDS             complement(2306..2728)
                     /locus_tag="U4A12_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774962.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2908..3279)
                     /locus_tag="U4A12_002633"
     CDS             complement(2908..3279)
                     /locus_tag="U4A12_002633"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1774963.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(3613..3876)
                     /locus_tag="U4A12_002634"
     CDS             complement(3613..3876)
                     /locus_tag="U4A12_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774964.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1328 a          878 c          651 g         1464 t
ORIGIN      
        1 tttaaaaata tataactgct aaatttcttt tttttaattt atttacattt ctgattacta
       61 cagaaaacta actacttttt ttatgtaaag ttctgtcaaa tatagtcact ttatattact
      121 ttctcttgag taaaaaaaca tgtgaggtcg taaacaaaga ttgaactatc tacattcata
      181 ctctataatc tgtttatttg cagactgata atagcaaaag aaaacttata atcaaataat
      241 atcaacattt gatcacataa tttgagattg aatgctgatt tacctatgtt ttacgtaatg
      301 gtctttagtt atttttgctc ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa
      361 tttcttggct ctataatttc taagactgat aaaaatattc tatcggttct ttttgcaaat
      421 aaaaaaacat atagaagctc cattagattg aatcaccaca acacaatcaa aggagcattc
      481 cgtatgcttg attttcattc tataaaacta cttttaggaa tcaaagaaaa tcacaacggt
      541 tcaaactaaa agcaagaata tccgaagtac cattgtttca tcgatcatga gaaaagcatt
      601 cacctctgtc tgcagatgtt cttatatcgc tctgtcagtc ctattaataa agtactgttt
      661 tgttttcaaa aaaactatta cgagtacttt ttaagagaat gccttgcaaa tattcatcac
      721 acaccgttat ataagtcaaa tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt
      781 ggcattatca gttaagtagc aacttaattc tcggacttgt tctactctag caaaatttat
      841 tttcatcaac ttacgtccaa atgttttttt gaataattac ttcatcgcgg gtctggaaga
      901 cttgcctcat tcgataagtc actagctttt ggcgtccttc aggatttcta gtatacacca
      961 actgacattt gggcagcgac gattgattta attcgtttag aaaaaggtta gcgatttgct
     1021 tatttcggcc aaagcgttct ggtaccgtca gaactatctt tttcccttcg attaaatacc
     1081 gcggattctc cagcggtgct aaaagctccc tcatagcttc agtaaaaagc tgtccttctc
     1141 gatagccagc attctcaaga tagcatctaa attcctgctg atcagccgct acgattactt
     1201 ttctatcact aacttcacca aggcgctgca acgtcgtttg aacgcatatg gccaagcttt
     1261 ttttgcgggc acattccttt tcccatagct tttgtcgcct acgaatttgg aaatattcca
     1321 gcaagttacc tttaaatata gctgaccaga agcttagagg ctctatattt tttactacta
     1381 tcggcaatga agtttcttgt ttttgcttta cctgatcgac tttcctaacg acgattggca
     1441 tagataggtg ccagccatca gctaaagcag tactccattg ttctttcaac cgacttcgtt
     1501 cacaagaaaa atagaacatc gactgagtat aagtttccat cgtggtggtc gtatattcca
     1561 gtggtaaagc aaagcgatcg ccaccactct caatttttgg tgaagattca aaggtcagac
     1621 ctgaaatatt gcccaaacgc cgtagcattc ttttcaaatc aggctgttca gacagaggaa
     1681 ctcccggtaa tccaatcgct aaatgccaaa tgttcgttac tttattttgt gcacctaggc
     1741 gtaagccccg ccctcgtagt tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga
     1801 tcaacagatt aattgcagga caatcccagc cttctcctag caaactaacg gttcctacca
     1861 aaatattcaa cttcttttcg gttaataggc gagtaactgc ttgcatcaat gcttggcggt
     1921 ttttgtcatc caccgtcaaa taaaaataac ccgacatata aggatgttca ttcatacccg
     1981 tcgctagttc tgccagctca acagttacta gcattttcgg taacagtaag aactctccac
     2041 agatggcacc gatttgctga gtgttaccta atagacgaac aacttcatca aacaaaggaa
     2101 ccacgccata agctaaggta ccatcactgc ctgctaatac ctccttgcaa atacgatcca
     2161 tcaaaatgac cgctgtcagt tcttgcttca gctgaacttc ttcttttaac aaaatttgac
     2221 aaactgcttg ctttttctca ggaatcgcct gactaccaac agtgtctgga tactctggaa
     2281 acaaatgcag cgactcatca aacaatcatc cagctttttt caattgataa aaaataggca
     2341 attccggttg atcttcaaac atctgctgat aaatagtgat cagctctttt ttacctaaag
     2401 atggaactga agctttttta ccttgctgct tagaactgcc taatagctgc caatgttcgg
     2461 gtatcaattc atagttatga tgattcaagt aaattaatgc actagtgaac agctcaaaat
     2521 tttcataaat ttccttcgaa tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat
     2581 aatccaccgt ctcttggctt tccattaacg cttccaccag ctcattttgt ttctctaaaa
     2641 aggtaaaata atgctgagta gtttcctgat tagccggaat caaatataga aaatcctgat
     2701 gaggacatag aacctcctcc tttaccaact cgggaatgct aatttcttcg tcaatcaggc
     2761 cattttaact caatatattg cttccactcc gtctgagagc tatcaatcgg aggcgtggct
     2821 gtcaaggaaa tcgtcaacag attcggttgt tttttcttca agtcaatcaa caattcaccc
     2881 cagctttttt ttaaatgatg agcttcatca aaaaactaac agctccagct gatcaccata
     2941 agttggctcc tccttcagtc ggttgtaaat ggtctgatac gtttccactg taatatgact
     3001 aggatgttca atcgccccag aaagatagca actatctgct ggatttacta agaaatattc
     3061 ctcgattgtt gttaaccact gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt
     3121 acaatccaaa cgtcggatca tctcaattcc taaaaaggtc ttgccagccc ctggtggagc
     3181 cactagatgg actgaattgt tatttaaata agtagcaaag ttagtcaata tacgcttctg
     3241 atagctacgc cactgaattt tttcatcaga tagccacatc ccaggtcccc ctctcacgta
     3301 ttttctctat cataatctaa atgaatacag caaaaaaaag atttcttcaa gaatatgtgt
     3361 ttttatagta atattttact tactctaaac tcaaatgaaa aggtaaaaaa actggtagta
     3421 caatctttta aattctcatt gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg
     3481 cgtttctacg cgtgaccaaa atttttagct ctataatttc taagactgat agaaaatcat
     3541 tctgtttttg aagtcttttt tcataaatac tacttgattt attgtgtata ttgtataaaa
     3601 gattgttcat aattattttt tataattaaa atttggtatt gatgaccagc gattccgtat
     3661 ataatgcgca atcaaattaa gctttctgtc tctggtaaat attcctgttc tgttttcttg
     3721 tactcgtaag atttcttcac ttatcgcaaa attcataaaa ttccacactt tttcataaac
     3781 aaaatgtaca catttatcta ttacttcata atagtctact tgatattctt ctgtaaacat
     3841 ggttgctact gtatctgcac catatttgat aaacatttgg gctactccct taaattgaga
     3901 aaagcccatt agtttatcta ctaatacttc taaaatttct tgctttcttc tttaacatcg
     3961 tcccgtaagg aatcatgtag tatgctggag gaagttcatt ttctaaataa ttgtatcgct
     4021 tcaattgctc aaaggctatt ctctacaatt aattacacca attactttat cttcacttat
     4081 ataagatttc taaacatttt tcgtcaaatg aagagaaaat atttttttaa atgatcttgc
     4141 tcaagatttg tcaacgaatc tttgaattcg ttttgaagtt ctgaggaagg tatcatttaa
     4201 aaactcaatc tgactttcat catttcgttt actcccattt tgatcacctt ctaccaacaa
     4261 tttattaaag tgattatact aggtttttat ttttttaatt cacaattgtt tattcacttc
     4321 a
//
LOCUS       DASJOX010000168         4302 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJOX010000168 DASJOX010000000
VERSION     DASJOX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.172"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..338)
                     /locus_tag="U4A12_002635"
     CDS             complement(<1..338)
                     /locus_tag="U4A12_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774965.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="U4A12_002636"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="U4A12_002636"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN1774966.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="U4A12_002637"
     CDS             complement(1495..2922)
                     /locus_tag="U4A12_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1774967.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="U4A12_002638"
     CDS             complement(2953..4167)
                     /locus_tag="U4A12_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1774968.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1349 a          917 c          768 g         1268 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tc
//
LOCUS       DASJOX010000169         4282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJOX010000169 DASJOX010000000
VERSION     DASJOX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.173"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="U4A12_002639"
     CDS             complement(37..1257)
                     /locus_tag="U4A12_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN1774969.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="U4A12_002640"
     CDS             complement(1277..1888)
                     /locus_tag="U4A12_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1774970.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="U4A12_002641"
     CDS             complement(1931..2863)
                     /locus_tag="U4A12_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1774971.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="U4A12_002642"
     CDS             complement(3284..4144)
                     /locus_tag="U4A12_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1774972.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DASJOX010000170         4252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJOX010000170 DASJOX010000000
VERSION     DASJOX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.174"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..858
                     /locus_tag="U4A12_002643"
     CDS             <1..858
                     /locus_tag="U4A12_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1774973.1"
                     /translation="QNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            875..1309
                     /locus_tag="U4A12_002644"
     CDS             875..1309
                     /locus_tag="U4A12_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774974.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1926..>4252
                     /gene="esp"
                     /locus_tag="U4A12_002645"
     CDS             1926..>4252
                     /gene="esp"
                     /locus_tag="U4A12_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN1774975.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1562 a          590 c          798 g         1302 t
ORIGIN      
        1 caaaatttga ctttgtattc tttaggagac attcgagata ttctgatttt agttcattat
       61 ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata tacagaaaat
      121 ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca
      181 gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt
      241 cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga
      301 gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg
      361 tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa
      421 gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt
      481 tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct
      541 gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat
      601 tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg
      661 atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac
      721 catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga
      781 gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat
      841 ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt
      901 ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag
      961 ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga
     1021 caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca
     1081 tattaatggt agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat
     1141 atcaagttat ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca
     1201 gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt
     1261 aaacaactac aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta
     1321 gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat caataattta
     1381 atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata
     1441 actccaatca ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt
     1501 tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac
     1561 ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac
     1621 tctgaagttt aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg
     1681 aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt
     1741 taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc
     1801 atgaaattct ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata
     1861 atacgtatca gatttatctt ttattcttac taggaaagta attattaagg gagttgattt
     1921 gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat
     1981 tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc
     2041 ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac
     2101 ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc
     2161 aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag
     2221 aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata
     2281 atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta
     2341 aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct
     2401 ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc
     2461 gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg
     2521 gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc
     2581 tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa
     2641 ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata
     2701 gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat
     2761 atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta
     2821 taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg
     2881 gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta
     2941 tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa
     3001 caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt
     3061 caataactaa taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag
     3121 tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta gaagatatga
     3181 aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt
     3241 ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt
     3301 tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc
     3361 catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga
     3421 aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag
     3481 gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag
     3541 atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat
     3601 taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta
     3661 aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta
     3721 aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag
     3781 agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa
     3841 atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg
     3901 atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta
     3961 catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata
     4021 ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag
     4081 ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc
     4141 aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa
     4201 atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DASJOX010000171         4233 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJOX010000171 DASJOX010000000
VERSION     DASJOX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.175"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            119..625
                     /locus_tag="U4A12_002646"
     CDS             119..625
                     /locus_tag="U4A12_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN1774976.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="U4A12_002647"
     CDS             642..1541
                     /locus_tag="U4A12_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN1774977.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="U4A12_002648"
     CDS             1628..2512
                     /locus_tag="U4A12_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1774978.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="U4A12_002649"
     tRNA            complement(2704..2775)
                     /locus_tag="U4A12_002649"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="U4A12_002650"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="U4A12_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN1774979.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
BASE COUNT         1531 a          771 c          850 g         1081 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gca
//
LOCUS       DASJOX010000172         4215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJOX010000172 DASJOX010000000
VERSION     DASJOX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.176"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..612)
                     /locus_tag="U4A12_002651"
     CDS             complement(<1..612)
                     /locus_tag="U4A12_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1774980.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWL"
     gene            complement(654..1712)
                     /locus_tag="U4A12_002652"
     CDS             complement(654..1712)
                     /locus_tag="U4A12_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEN1774981.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(1831..2286)
                     /locus_tag="U4A12_002653"
     CDS             complement(1831..2286)
                     /locus_tag="U4A12_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774982.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(2870..3673)
                     /locus_tag="U4A12_002654"
     CDS             complement(2870..3673)
                     /locus_tag="U4A12_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1774983.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1250 a          810 c          565 g         1590 t
ORIGIN      
        1 tagccattgt ctcaaaatat cgacgacaac atcgcttttt agttcattca attctaacgg
       61 ttgtagccgt ctccctaagg catcatctct tttgatatag ttttcatatt cttttcttgt
      121 agtagcggta atcatttgaa gtgattcatt tgctaacggt tcttttagcg aatcaccccc
      181 aattttattg ccactcccaa aagagctaac aactttatga aactcgtcaa tgaagaggac
      241 aaattcatta tctttgtcct cagcttttaa aattcgctca tattctttca atcttggaat
      301 taatgtttct aaacgtgttt ggaggacatc atctccccca acacgcaatt gtccgattga
      361 taatttatat acttgtactt tctttccatc ttgtcgctgt ctttcagccc attcacttgc
      421 aagtgctgtt ttacctgttc cggcttcacc taacaagata gcttgcggtg tttccggtct
      481 attcaagatc agtgacaact catggagttc gtcatcacgt cccactaact tatgttttgg
      541 tggtttcatt ggtgtgagag catgattaaa ttcggtttga atattcttaa aaaactcggt
      601 actattttcc atctgtttgt gttcacttcc tctaaaataa gaataattta cttctagtca
      661 gtcaaagtta ggtacttaaa tgtcgtcttc atgtctccaa aagaatcttt gttaaagtca
      721 gcatttttgt attgatcaat caatttttgt gtatcattga ttgctttaat ttcggtctcc
      781 attcgactgt ctagttgacg aatactttct tgcgaatctt tatcgtcagg attttgttgt
      841 aaacgttcca gatactcagc ttttgtggct tcaattttct tgatgttagt atttgaagca
      901 tttaactgat cttctagctt atttacaaca ttattcttac ttgtaactgc tacaactttt
      961 gaataatcaa tatcgccatc tttggtaaga aagcttcctt gatagacatt agttgaatct
     1021 gaatatggat ttattctgaa attgatatta tcaggagtat taacaggttt tgcttttttc
     1081 ccaaaagaaa ttactccttc attattaaca tcattcatag aatattgaga aagtgctttt
     1141 gtaactgaat ctgcttcatt cacattgact ttggtatcat cgtcagctgt atcatcacca
     1201 gaaattagtg atacttggtt tgcaataaaa acttgataag ctctttcctc taatttacct
     1261 tttaggaata agaaagcatc cccatcagta cttaacatcc catagctcat ttcaagcttc
     1321 ttaggacgat ctttttcttt tgttgccata tataagttat agttttttcc ttgcgtagat
     1381 aacaactttc tagcgttttg ggagtaacca agttttacta ctgttacatc tctattttga
     1441 tctgtccaca catctgaaat agtgatttct gctcctgatc gagcaaactc caatgaagac
     1501 cctataggtg ttgtattgtt cacgagataa taattatagt ttcctaattg ataaatgtag
     1561 ctacccaagc ctgttaataa aaaaagcact cctagaaaag ttgcaagcat aatgcgtaat
     1621 ccgtgacgtg ttctcttatc cttatcacca agcaaccctc caataatact gtattgatct
     1681 ttggtagatg ttgaattgac gtttttttcc atcagtttgt ctgccccctt tcataaaaga
     1741 gcaaacaatc agacgttttt tcggtaattc taggttcggg attttgaatc atttcggtat
     1801 attcttcccc attaatattt aacacaaaaa ttatcttcct ttcttcgttc ctttcttttg
     1861 ctctttattt tccaaataat caatgatttt tgctattgta ccaagatagt aattcccctc
     1921 agctttgttt tttcggattt tgctaaccat tggagtaggt acaatctctt catctctcaa
     1981 ttcactgaaa gattttttac ctagatattt tccaataaca gattttaaaa aacgttcaaa
     2041 atctttctcg taagttgttt tcttgccttt tagttcatgg ttcatattgt caacgacttt
     2101 tgcggtttca ataattgttt tagttctcag cttaataatt tcatctggaa ttttcaatcc
     2161 tcgtaatttc atcgttgtac taactggtaa cccatgcttt actgcatcag caatactaaa
     2221 actttttaga tcatctagta agctctccat atagttaaca aattcatgcc aattaatagc
     2281 attcattgtc tcaccccttc atttttttaa aatgaggttc gggcgtaaca catttatgtt
     2341 attataatat cataataaca taaataaatc aatcagcaaa tttagatatc ccttaaagta
     2401 tcagcaatta ttcaacaaga tatttatttt agctatttca gtcatcataa aagactaatt
     2461 agcaataaaa taactaatat tatccattta tcagacgaaa agaaaaaact tatctaatta
     2521 ctctatatta acttttaaca aatagttgac ttgtccaaaa atgttacctt caatctcaca
     2581 aagatattaa gatgtttcct accaaaactg atatcaccgg actttttatt ctgtgttgtc
     2641 caaaagtgtt acctttagtc gatcaacaaa ttaaaaaaaa ttgttttttt cttgacttgt
     2701 ccaaaagtgt tacccatagt tgttaaaatt tgtatagatt agccattctt tcgaatatta
     2761 tttagactga aacttgctta caaaaaaact tcttgcattt attgtccaaa agtgttaccc
     2821 atactttttt aaagaagcag gttgtattaa aattacaacc tgcttcttct caatcttctt
     2881 ctaaccaatt atgcattgaa atggttggca agtttgattc tggctcacta aaagtgaatc
     2941 tgagccgatc aactcttctt cctttctttg catagatttt ttcataatca aaagtttcaa
     3001 atacctgtat tccgttttca tcaggagcta aaaattcttc tttcgcctgc tctagtattc
     3061 ttttgtcaat atttgtcatt ctataacttg aaggtatact taatgctctt ctaaaatctt
     3121 caactttgac tacccaataa ccatttcgcc actctttatt tctgtattgc atgagatgac
     3181 gatacaattc ttttgtgtag ctacttctta gttttgtaaa attatcaagt tcaaaacgag
     3241 taaagttttt gccaattgag tttaagataa attcaaattc ctcatttgcc ttaattgtaa
     3301 ctgtttcctt ctgtttatct atccggaatt tttgaaaaag ccacagctgt tcatagacgt
     3361 ctgaatcttc aaaacggtag gttaaagtac ctagtttgtc actcaaagct tttaacacag
     3421 aataaaaact gtccttttct cttctgtcgt atttgaccaa ataacgaaag aggttgaaat
     3481 caaaagttac ttctgctgtt ccttgacgtt tcatttttga taaaatagcc caaaacacat
     3541 ccatttcaac aggcttaaat cctcgcaaag ctgttgtatt cagaccattg ttataacgca
     3601 caattgcatt tgtttgttct gatactaaat ctacatcact atcaataatc ttttcggtaa
     3661 cttcttgaga cattgattac tctccaatct tctttttgcg ctcctttaat catgttaatc
     3721 tggtactaat taaaggaaac aattttttct gcttgttacc taaattaaat cacaactaaa
     3781 ggtaacagtc aacaaagatt tgttactaat ctaattttct attttttcgc cttccaaatc
     3841 aagaaaaccg aaggtaacgt cttatcctag tgtgatacct caaatgtcca cttttgttac
     3901 ctttgctgtc caaaagtgtt acccttactg tccacttttg ttacctttac tgtccaaaag
     3961 tgttaccttt ataccccttt aatacttact ctcccaagag attaagcctc ccctaacaga
     4021 tatattaaca gatatttaac agatatatta acagatattt aataaaggag gattactcct
     4081 ctcttatttc aaaatcttaa aaataaaaaa tcttaaaaaa aactaacact atctaggagt
     4141 ttattttgct tgtgtaactt tgaataattg aaaaaaagga aaaggaggaa gcttcagctt
     4201 ccccttttat ttata
//
LOCUS       DASJOX010000173         4181 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJOX010000173 DASJOX010000000
VERSION     DASJOX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.177"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..1348
                     /locus_tag="U4A12_002655"
     CDS             47..1348
                     /locus_tag="U4A12_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1774984.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="U4A12_002656"
     CDS             1375..1635
                     /locus_tag="U4A12_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1774985.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="U4A12_002657"
     CDS             complement(2634..3869)
                     /locus_tag="U4A12_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1774986.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1206 a          672 c          870 g         1433 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataatt
     4141 atattaattg ataggtgtgc tgtcgtcatt atacacacgt a
//
LOCUS       DASJOX010000174         4139 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJOX010000174 DASJOX010000000
VERSION     DASJOX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.178"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            402..734
                     /locus_tag="U4A12_002658"
     CDS             402..734
                     /locus_tag="U4A12_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774987.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            859..1116
                     /locus_tag="U4A12_002659"
     CDS             859..1116
                     /locus_tag="U4A12_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEN1774988.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            1348..1752
                     /locus_tag="U4A12_002660"
     CDS             1348..1752
                     /locus_tag="U4A12_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN1774989.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1771..>4139
                     /locus_tag="U4A12_002661"
     CDS             1771..>4139
                     /locus_tag="U4A12_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1774990.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPT"
BASE COUNT         1535 a          546 c          747 g         1311 t
ORIGIN      
        1 cgttatgtag taaatgtaat agtttttttg gtagtgagta tgttgaggaa tatattagat
       61 ttgcagaaga caataaaaat ttcctttata acaatacaag tttaaagaat ggtaagtctg
      121 atttaacaca ttcaataaag aaaatgaatt cacttagagt tgcaaaagca attgtaggaa
      181 tgttttttag tttaaatggg gatgaagatt caatggataa gccattctta gattcggtac
      241 gagaatatct tagcaatcct aaaagtactt tatttcctca tgaagactac aaaattataa
      301 tgaattatta tcttccaata ggtgctccac agaattctaa aatcaaaatt cctaatgaca
      361 ttgtaaatga tggtgacact cacaatgtaa taaaaggttt tctgcccctt gatctatcaa
      421 ctgggagaaa aattcgattt tcagaaattc agtatgaagg aattggtttt actcttatag
      481 atttaaaaaa ttcaactttt gaaataaaag aaggattaga tttaaaaaat ctcctttgtg
      541 cagatgataa aatcaaaaaa atacaattgt acaaagtccc tatattaaca ccaacagtac
      601 ttacaatgtt ggagatggaa aataatatta attatattga ctcaggttca gatttgctga
      661 ttaggtggca ggtaataact aattttctta tggaattaaa aaaatacagt aatactcaaa
      721 cgaagttctc ttgaagttat aaagaataca ataaatataa atttccttta gactatatta
      781 aaatcagcaa tcgaaggaaa ataaaagaag aaaatggtac gcaaaatcta actattagag
      841 caaaaacagg aagctgcttt ggaagatagt cccgaattgt ttccgatgtt aaaagatcgt
      901 ttaaatgaat tgactgaaaa acgaagacag ttacaaattc gagagaatga aatcttaggg
      961 atctttcaac aacaagggga accgattcaa gtcaaagatg tccaacagat attgactagt
     1021 ttggattgct ttcttatcca gagtgagaag aagcaagtta aagctttgta tcacaccttt
     1081 attgagaaaa tcacctttga tcctaatcag aagtagatat tgccgatccc gatgagattc
     1141 acacctactg tgatttgata attgaatgag taatatcaaa tagccgtatc caaaccaaag
     1201 gatatggcta tttttgtgtt agggatatac gtcacgtagc ttgttatcaa gaataagttt
     1261 ctttattttt ttgctttttc taaaactgag tatgctaaaa taagagggta tatagcaact
     1321 ttaaataaga gataggagtg tatctttttg ggtgcagaat taaatcagaa gttatttagt
     1381 gcggcagata atctccgtag taaaatggat gcgtcagaat ataaaaatta tttattgggg
     1441 ctgattttct ataaatattt atccgaccgt ttgttggaac aagtggttct tttagcagat
     1501 gaatccttag aggaatatga cacagtaagc aaacaaacga tgttgtatcg tgaattactt
     1561 tcagatgaag aatcaaaaga agatctgatt gcaacaatcg ttgatatttt ggggtatgcg
     1621 atttcgccag agtatctttt caacgtctta gcggatcaag caaaacaagc aacgttccaa
     1681 ttgaatgatc taaacaaagc ctttttagca agtcatattt atggaaaaat actgtcggca
     1741 aaattcgaat agtgtcgagg aggtgctgtg atggaagaat taaaagaacg cattgtcaat
     1801 tatgtcatag agaactctga gattaaaaat aaatggggga acaatacagc acagaaaata
     1861 tctgaaaaat taaatgttag tcgtaatatt gtctctcaat atttgaatga attcgtaaaa
     1921 gatggagaat ttggaaaaat caatactcgt ccagtaatct tcttttcttt accggagaaa
     1981 gttccaaatc aacaaatttt tagctcttca gaggaagcgg aaaaatggta tgatagctct
     2041 tttagggttg gaatagaaag ggttatcgga agtcaaggaa gtctgcgaaa tgtaattgct
     2101 caaattaaag cagcaatcta ctatccgcca aatggcttac ccattttact tcatggcgag
     2161 actggaactg gaaaaagcta tttagcaaat tatgtgtata aggatttgtt ggccaataat
     2221 tatatagaag agaaggcatc tttcatttca gttaattgta gtgaatatgc caataatcca
     2281 gaacttttct tgtccaattt ttttgggcat gtaaaaggtg catatacagg agccgaagat
     2341 ggaagaaagg gtttagcaga attagctaat aatggagtct tatttttaga tgaaattcat
     2401 gcattgagca atgaatgtca ggagaaactt tttcagttta tggatacgag gaaatttcat
     2461 cggatgggtg ataatgaaaa ctggtacaca tcaagttgct ggattatatt tgcaacatca
     2521 gaaccacctg aggataaact attatcaact ttgttgagaa gaatacctgt aaagctaaca
     2581 cttccaacat tagacagtcg ttctctttat gaaagaaaac agttaatcaa gatatttttt
     2641 aaatcagaag aagaaagact agcaaagaaa atactgatat ctcaacaatt attagatttt
     2701 ttattaaact atcgctttag tgaaaatgtt ggcaatttga aaaatatcat tcaattgacc
     2761 tgtgccaatg ccttttattt cttagatgat ttggaagaac tttatattca gatgtatcat
     2821 ttaccacaat gggtgaattt caacggcatg aaggttatga aaagacagaa tgaattaaaa
     2881 tatattcaaa tggatgattt ggaaaaagta gacgaagttt ttaatatcaa cgaacttata
     2941 gagtatcttt atcaaacgtg gaagaaagac actgataagc agcagctttt tgagaaatat
     3001 gatagatatt ttagacaaaa gaatgttgat atccaaaata attcagcttt aacatatccc
     3061 acctatcaag agattcgatt tagacagtat gttaaggaaa tagagagaga ttttcaaata
     3121 gaatttgact attattatct tcaaataacg tctatctttt tgaattcttg gatttatcaa
     3181 atccaattaa catcggacga taaattatat tccttcatca atgatattaa tagttggtgt
     3241 agtcgagaag ttcaattagt caatcgtttt attcaaatag gggatattta tttaacaaaa
     3301 attcaaaaaa atgttttggt tattttattt aagttaatgt gtagtaaaga atttcagcaa
     3361 aaacgtttag cactgatttt ggctcatgga gatctaacgg caacctgtat agcaagtact
     3421 gctaatagga tcgttgagag atatatatac gacgccattg atatgccact aatgacttca
     3481 agtagtgaga ttgcagacaa aattaataga tacttacaag agtgtaccgg atatgaagaa
     3541 attattttac ttgttgatat gggttcactg gagaaaattt atctaaattt ggaactagac
     3601 gctcaaaaaa aattcggact actaaacaat gtttcaacaa gaatggctat tgatgttgca
     3661 aatcagatat tattagattt accgcttaaa cagattcttg aacaagtaaa agaaaatcaa
     3721 aatgtggatt atatttatga atcatcaaat attaagaagg atatgattgt ctgttcttgt
     3781 gcaagcggat taggaacttc gtttaaatta aaaaagatac ttgaagatag ctttccaagg
     3841 gatactgatc ttgtcataga aacgtgtgac tattactctt tagtgaatga aaactattta
     3901 tcggagcttc gtaaagagaa caatattctt tttattgttg gaacactaaa tccacatcta
     3961 gaagatgtag aattcttttc aattgaagat atgattttag gaatgaatat aagtaaattc
     4021 tccaatcaat tgaatagaca ctttaatgat aatgaactga aagttttgaa caataatatt
     4081 ctgaagaatt ttagtctaac gaatttaatg gagcaattga ccatattaaa tccgactaa
//
LOCUS       DASJOX010000175         4069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJOX010000175 DASJOX010000000
VERSION     DASJOX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.179"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(182..1687)
                     /locus_tag="U4A12_002662"
     CDS             complement(182..1687)
                     /locus_tag="U4A12_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1774991.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1684..2370)
                     /locus_tag="U4A12_002663"
     CDS             complement(1684..2370)
                     /locus_tag="U4A12_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1774992.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2563..3984)
                     /gene="gndA"
                     /locus_tag="U4A12_002664"
     CDS             complement(2563..3984)
                     /gene="gndA"
                     /locus_tag="U4A12_002664"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1774993.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1120 a          859 c          694 g         1396 t
ORIGIN      
        1 atattacagc attttattgt tttttcgttc atctaaaaga tacttacatt taattaacgg
       61 atgttattct atttaattgt tcgctagttt ttttataatg acatccctga atttctcact
      121 tgcttgtatt ttgtacctta ttctttgttg gatatataaa aaaacaagta aagcacatgc
      181 tttacttgtc ttctgccttt gcgataggaa taaagattct aaaaattgtc ccttgcccta
      241 ctacactttc tgctaagata cgtcctttat agttttcaac cagttgttta gcgatagaca
      301 accccagacc atttccaccc ttcgttctag ctcttgcttt atccactcga taaaaacgat
      361 tgaagatctt attaaggtct tctttgggta ttccttcgcc aaagtcctga atcgcgatct
      421 caaattcatt cagagtggaa gaaatagaga tatggacttc ttttcgtgta gttgaatact
      481 ttactgcgtt atccaatatg ataatgatta attgttcaaa gtgattacga taaatttgca
      541 gggtcacttc ttttgtcaaa tcatcatcta aagtgatcac atagtctgga tataatatct
      601 tgaaattatt ataaacttga taggtgactt cttttccttt agttgtttca ttcgcataat
      661 gaacatctac ttgttctgct cttgataagt ctagcatctc ttgaactaag cttttcatac
      721 ggctgatttc ttgcatgctg gcgcttaatg attcttctaa tatctcagga tcatctttcc
      781 cccaacgatt tagcagacta agatgccctt caataattgc aacaggtgtc cttaactcat
      841 gagatacgtc ttccacgaat tgctcttgtt gttcaatata caaacgcata cggtcaagca
      901 tctcattgaa tatttcagct aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt
      961 ggatatctga ttgcggatct ttacgtatag tatccattgt atctctgagt accttgagtg
     1021 gttttaagaa ataagaagat aaaaagaaac ctaaaatact acttaagatc aaagaaacaa
     1081 cttctaaaac aataagagtc aataatagct tatttcgaat atcatagaaa gaagataatt
     1141 catagaagct ttgcgcatag ccgatctttt ctcttgtacc tttagaatat ataggctgga
     1201 tagataaaaa gccagttttt ccttctagtg taacgatcgt tggaacttta cgtgtagtct
     1261 gaatcaggtt tcgactgttt tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa
     1321 cataaagatc cattgatggc tgccctagct cagctataaa ggaatcgatc ttcattaagt
     1381 ttccttcgac cagtgaagta tgatctacga cctcatctcc gtcatttgtc gctttcgtca
     1441 gattacgata cgtatttact aatgtcagtt cttcatccga gttagccaaa cgagaagcta
     1501 cttctgaaat cgtacgctct acattgtttc tttcttttgc cacaatcaga ttgatagatg
     1561 atttataggt aatgacagcg aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa
     1621 aggcccactt gattgtcaag gagggccctt tcagttctct tttttccgat tttttcggct
     1681 ttatcacgag cgcatcacat aacctgttcc gcgtacagtt tggatatagc tttcctcccc
     1741 gggaacgtca attttgttgc gaagataacg aatatatacg tccactacgt ttgtttcaac
     1801 ttctgtttcg tatccccaca ctttatttag taaaacgtca cgtgctaata ccacgttcac
     1861 gttttccatt aaagtaagca gcaactcgta ttctcttttt gttagttcaa ttacttcatt
     1921 tccgcgacgt acgacacggt tttctttttc gatagtcaaa tcgcgataag taatagtagt
     1981 ttgtttagcc acatttttat cgccttcaat atcgattcga cgcaacaatg cacgaagacg
     2041 tgctaataat tcttcgatag caaatggttt gacgatgtag tcatctgctc cgtggtcaag
     2101 tccagataca cgatcgatca cagaatctct tgcagtcatc ataatgattg gtgtattttt
     2161 gacttggcgt acacgtctgc agacttctaa accgttcaat tctgggagca tcagatccaa
     2221 caatatggca tcccactcat tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg
     2281 gacttctgcg ttataccctt catgcttcaa ctctagctcg acaaatcgtg ccaagttctt
     2341 ttcatcttca atgattagaa tattgctcat ttattatttt atcctttcta ttctactggc
     2401 tttttaaaaa ctctatcatt ataccgataa tatgcttaaa aatctatagt cattttatca
     2461 tggatagcaa ttttaaaaaa gagctgaagt ctactcataa caatcgactt cagctctttc
     2521 gtcctctttt tattttctca tttcctaaca gccgaaagac tattattctt catggtacca
     2581 tgagtaatgg aagattcctt ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc
     2641 gcgttgggct tggattatat ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc
     2701 gatggctgat gagaaagtcg gtacaggtac gccagcttgg acagccaagg caacgacgtc
     2761 tctgactgat tgctgatatt ttttcgtgat ttcaacgaag taatcatcta gcaataaatt
     2821 ttcgatctca ggatttttgt cataggcatc cgtaattttt tgcaagaatc gagctcggat
     2881 gatacatcca gcgcgccaga tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc
     2941 ttttgaagcg acacgtaatt gagcaaatcc ttgggcataa ctcatgattt tactgaaata
     3001 caacgcttct cggatttttt cgaccaattc ttttttgtct cccgtgaact caaaatcgtt
     3061 cgtacgagac aaaatcttgc ttgcttgtac gcgttcttct ttataagcag aaatatagcg
     3121 ggcaaataca gattcagtga tcaatggaag tggcacacct aagtcaagcg cgctttggct
     3181 tgtccattta cctgttcctt tattcccagc tgcatcaagg atcacatcta ctactggttt
     3241 acctgttcct tcatcgtctt tacgtgtcag aatgtcagca gtgatttcta tcaaatagct
     3301 gtctaattcg ccttgattcc attctttaaa gatatcagcc atttcttcta ctgaaaggtt
     3361 taaaatattc ttcattaagt cataagattc agcaatcagt tgcatatctc catactcaat
     3421 accgttgtgt accattttca cataatgtcc ggctccattt gggccaatat atgtgacaca
     3481 tggtgctccg tcttctgctt ttgcagaaat ttgctctaag attggtgcaa ctagcgcata
     3541 ggcttctttt tgtccaccag gcatgattga aggtcctttt aaagctcctt cttcaccacc
     3601 ggaaacgcct gtaccgataa agttgatgcc agagttggct aactcttcat tacgacgcat
     3661 cgtatcttta aagaatgtat ttcctccgtc aatcaaaata tcgcctttat caaggtgagg
     3721 aagtaactct tggattgttg catctgttgc cggtcctgct ttgaccatta gcaagatccg
     3781 acgtggtttc tcgatagatt ctacaaattc ttcgattgta tatgttgctt tgaaattttt
     3841 atctgggtgt tcttctacta cagcggttgt tttagcccct gtacgattga ataaagccac
     3901 tgaatagccg cggctttcta tgttcaaggc tagattttta cccataacgg ccataccgac
     3961 aacgccgatt tgttgttttg acatatgaac tcctcctaca tacgggattt ctccctaatc
     4021 tcttacatta tagcttaaca cggctacatt gcaaactaaa gaacaacat
//
LOCUS       DASJOX010000176         3987 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJOX010000176 DASJOX010000000
VERSION     DASJOX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.180"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(194..343)
                     /locus_tag="U4A12_002665"
     CDS             complement(194..343)
                     /locus_tag="U4A12_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774994.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            805..1017
                     /locus_tag="U4A12_002666"
     CDS             805..1017
                     /locus_tag="U4A12_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774995.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1295..3094)
                     /locus_tag="U4A12_002667"
     CDS             complement(1295..3094)
                     /locus_tag="U4A12_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1774996.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3218..3814)
                     /locus_tag="U4A12_002668"
     CDS             complement(3218..3814)
                     /locus_tag="U4A12_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1774997.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1426 a          705 c          684 g         1172 t
ORIGIN      
        1 taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt ttgtttaggt
       61 ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag acgtttgtcg
      121 cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt cgccatttgg
      181 aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg attgtaatac
      241 caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta gaagttgtat
      301 caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc atctcctttg
      361 taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc aagtttagca
      421 tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta ttttttctta
      481 aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg ttacaaatac
      541 tattgcttga atataaatca tatgctcacc tctttataaa taacttagca atataaagaa
      601 aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg tctaagatcg
      661 tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat aaaaaaatgg
      721 ataaaaacgt tttcaaatac aaggtaattt gttattataa aataaagaaa aaacaaaata
      781 aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc ttatatatgc
      841 aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata tatcaacacg
      901 gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa tgaaagcaag
      961 tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt ttcttaaaca
     1021 gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga agggagcgag
     1081 aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca gtgatctctg
     1141 caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa agttaagcac
     1201 acattatgag cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat gcgttttttg
     1261 ttgtataaag acttttttca ttaacgattt ttgattagcc gactcccaaa acaaaattta
     1321 gcagtttatc tgaataagtg atttcttcat ctgtttgttt cacactttca tttagtagtc
     1381 ccatctcatc ggttatgata ccatccacac cataaaacat catgcgactc atagcatctg
     1441 aatcgttgat tgtccataca tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt
     1501 tacggttgat cgtactatat tccattgtca gaaaatcagc aggcgtcaca gggggcccaa
     1561 caatattgaa aggtaagatg tagcccacat aaaagtttgg ttctgcttct tttaaatctg
     1621 aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata actttgcggt
     1681 atttatggac aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa
     1741 taagtaattt ttggtttagc ttatgtgctt tttccaaata agaatcaaaa gaacaaacag
     1801 gggcttgcac tccgttttca gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct
     1861 gtggtgttcc atttacccct gttaagtgac gaagatttgc atcatgcatc actacgaaat
     1921 gaccatcttt tgtttcctga acatccatct ctacataatc aggatgatag tctctgcttg
     1981 ttttttcaag tgcttctaaa gtattttgcg ccccattttg ggtactcaca cctctgtgag
     2041 agacagtcac tggttttgtc tgaacagctg aagtcagata atccatattg taaagacaga
     2101 cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac
     2161 gcaagttggg ggcttgtggg ataaaccact tagggatctc aggcaagaag ccttcgtcat
     2221 ccatgaaatc aacaatgata taaaaaatcc ctacggtaga catcacgata ttgaataata
     2281 aaatcccttg taaaagcgtc atagcaaaca cagcgctgat caatgcatag tctgggaaaa
     2341 attgttcaac catactttgt gccaaaataa caacgatata tcctaaagaa gaaaggagta
     2401 aaatcgtacc gccaatcaca ataaattggc ccgtgatggc caaaagacgc gatttggtca
     2461 atgaccagct ttcccgaatg gctgctcgaa aaggacgatc tcgtagaatc atttccggta
     2521 aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat ataaacgagt aaaaaggaac
     2581 tgacgatgat ccaacgatta gcaaaaataa aatccataat gaaagcgggg atttttattt
     2641 ttgacagtag atcagaatta aaacttaatc cgctgatcgg tagaatcaat aaaaaataag
     2701 caaggaaaaa caaaaaagta atgggtctca ctttcttcag ctgaagtatc gtcaagtgta
     2761 agagttgctt caaagagatt ggttctttct ttttaataaa aaaaacactc atcaaaagaa
     2821 aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa aactacgatt aacgagagta
     2881 gaaccccagg atgttgagaa aaaattgaag gaatattgtc ataagacaaa tagtcaattg
     2941 cccctcggtg taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa
     3001 taaagccatg catcagcaag acatcacgga aataagcttg tgttcctttt aaaaagtccc
     3061 aagcattacc taagctacgt ttcaaataat tcatcctatc acctgctctt cacattgtag
     3121 cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg cagacccttt cccagattta
     3181 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
     3241 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
     3301 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
     3361 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
     3421 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
     3481 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
     3541 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
     3601 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
     3661 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
     3721 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
     3781 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
     3841 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
     3901 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
     3961 aaagacttat ttaataactt gtttttt
//
LOCUS       DASJOX010000177         3962 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJOX010000177 DASJOX010000000
VERSION     DASJOX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.181"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1420
                     /locus_tag="U4A12_002669"
     CDS             <1..1420
                     /locus_tag="U4A12_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1774998.1"
                     /translation="IFCYQKEDDEVFFDEILNSIARGGGKNGFMSARDSFFISPLYGV
                     RNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMAIVNNVTNSIFSYRTNNP
                     KTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTFYNGTNGHVREGFYDKML
                     ERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPMFEEDSAYSKRLFSTVMK
                     EYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATKQELPNLNGRSCVAGFDY
                     ASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPIKDWEKKGLVTIVDEPSI
                     DPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYNFEFIRNIRGVQSKVAPI
                     IEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKEPVRRKTDGFHAFLAALY
                     KREEITDMDYEEAFDMLDEIEF"
     gene            1455..2537
                     /locus_tag="U4A12_002670"
     CDS             1455..2537
                     /locus_tag="U4A12_002670"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1774999.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            2581..3753
                     /locus_tag="U4A12_002671"
     CDS             2581..3753
                     /locus_tag="U4A12_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1775000.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            3740..>3962
                     /locus_tag="U4A12_002672"
     CDS             3740..>3962
                     /locus_tag="U4A12_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1775001.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYN"
BASE COUNT         1424 a          498 c          814 g         1226 t
ORIGIN      
        1 cattttctgc tatcaaaaag aggacgacga ggtgtttttt gatgaaattc taaatagtat
       61 tgcgcgaggc ggcgggaaaa atgggttcat gtcagcaaga gactcatttt ttattagccc
      121 tctctacggt gtcagaaatt atgatgtaac gattacagcg aattctgaga agcagggaaa
      181 ggtatctttt aaagaagttt atgaaatggt acaaatgcgc aaactggaaa aacaatttta
      241 tttgactaag atggctatag tcaacaatgt aactaattcg atttttagtt ataggacaaa
      301 caatcctaag actatggata gtgcaagaga tggctgctta gaatttgatg aaatccatca
      361 gtttgaatct tcagacctag ttgatattca gcgtagtggg ctagggaaaa taaaacatcc
      421 tcgaactttt tataatggta caaatggtca tgttagagaa ggtttttatg acaaaatgtt
      481 ggaacgagca caaaagattt ttagtggaga gaacaagaat gatcgcttgt ttccctttat
      541 ttgtaaactg gataacattg atgaaatgga taatcctaaa atgtggtcaa aggctaatcc
      601 aatgttcgaa gaagattcag catattcaaa acgtctgttt tctacagtga tgaaagaata
      661 taaaaaacta gaagatgagc catcaggtag gcgtgaattt gttgttaaac gaatgaattt
      721 tacagaaggt gatgcagaat cagatattac gactcatgaa aaattaatgg ctactaaaca
      781 agaattgcca aatttgaatg gtcgttcttg tgtggctgga tttgattatg caagtattcg
      841 tgattttgca acagtgggtt tgctttttaa aattgatgat aaatttatct ggcagcaaca
      901 tagttttgct aggaaaaagt ttctagatac attcaaactg aaagcgccta ttaaggattg
      961 ggagaaaaaa ggtttagtga caatcgttga tgagccatca attgatcctc gtcatttaat
     1021 tgattggtta aatgaaaaaa gaaaaatcca tcaaatagaa atcgtttgtg ccgacggatt
     1081 tcgtatggat ttgctgaaac ctcttctaga gaaagaatca tataattttg agtttatcag
     1141 aaatataaga ggtgtgcagt caaaagttgc tcctattatt gaagatggtt ttgcaaatga
     1201 acggtttatt tttggtgatg atcctatgat gcgttggtat acaaataatt cgtttatcaa
     1261 agaagacaaa ttagggaata aaacattttt gaaaaaagag ccagtaaggc gtaaaacaga
     1321 tgggtttcat gcttttttgg cagctttata taaacgagaa gaaataactg atatggatta
     1381 tgaggaagca tttgatatgt tggatgagat tgaattttaa ggtatactcc ttattgaaag
     1441 gggtagttat agaagtgaaa aagagatatt ttctatttgt tacaatattt ataataatag
     1501 caatttgtgt tttgacagta tttttaccac aaattactga gtatttaaat tatcggatat
     1561 ttaccacaaa agtagatgat agcgttacta gtttaagtag gctagaattt ataaaaatat
     1621 gtgcatctgt gtttggtcct attttgactg taatggtatt tgtgaatact ttaaatatgc
     1681 agaaaaaaac agagcaaaag caaaatgagc ttgaagtaaa gcaagctaag gctgagaacg
     1741 agagagcaaa aaaagagctg ttaacacaga tagatcgtga gttctatttt ttattaaata
     1801 tgtttattga aatacaaaaa gatcaaaatg ccaaacaagc tgttattgct ttacagaagt
     1861 tggcaatttt taacgaaaag ggttttaatc aagactatgc atttacttct aaagatttat
     1921 cagatagtga aattgggagt acaattgaac aatcaggacc gctgaagggc acagttgaat
     1981 atataaaatt tgaaacattt gatgtaagca agtcgatggg gaattatcaa acaaaaacaa
     2041 aagacgttgc tacaagttta cacgatcaga aagtagctat attgaatgaa caatatgaaa
     2101 agatgcaaat atatcttgga cggtacttca aaatgtttca ccgaatagtt aaaacattaa
     2161 atgagtatta tgacgattat aatgattttg atgtgaagag gtatacaaaa tatataggga
     2221 cattaagaac acaaatttct ccagctgaat tccaagtgat tttattcaat tctctatata
     2281 taaaacgagg ttttggctta ggaattcaac ttattggaag tggttttttt ggagatgatt
     2341 ttgattttga aactaatcaa cattttgaaa caagtatcaa tgaacagtgg tttctatcac
     2401 tatcaactgt tgacagtgat aactctataa agcgacaaaa attatctgaa tatattaaag
     2461 aattaggatc cgtagaatat aaaaaaatag ctaattttga aagtttatat aaactcttta
     2521 atgaaactaa gctctagaaa tagggctttt ttatttttct ttgaaaggtg gtgaaaacat
     2581 gtgagcttat ttgatatctt taagcagtca ataagaaatg aagagccttc tgagtggata
     2641 ccggattttg tatatggaga ggaagtatct gctcgggcat acctcaaaat tatggcaaaa
     2701 aatactgttt tagattttgt tgcaagaact atgtcaacgt tagaagttaa gtttaaaaat
     2761 aaagatggga catcagactg ggattatatt ctaaatgttc gacctaattc tgatatgtca
     2821 gcagctacat tttggcaaaa atttttttat cggctcttag atgacaacga agtgttagta
     2881 atatttacag atgataatca attgctgatt gctgatgatt ttagtcgaac ggagtatgcc
     2941 gtgtatgatg atgtttttac aaatgtgatg gtaaaaaatt ttgtctttga acgttcatgg
     3001 aacatgtctg acgtcattta tatggaatat aataacgaag aattagaaag gtttacacga
     3061 ggtttatttg aagattacgc cgcgttgttc ggacggattc tagaagttgc aatgcgtaac
     3121 aaccaaattc gtgggtctgt atccattgat tcaacgggaa cggcgaatga tgaaaaagga
     3181 aaagatggta agactagatc tcaaagatta caagaatata ttgataggat ttggaactct
     3241 tttaaaacta actcagttgc aattgttccc aaagtgaaag gctttgagta tgaggaatac
     3301 accaataaac aaggcgtatc gaatcaatcg ctagatgaac tgaataagat gaagtcgtct
     3361 ttaattgatg atgttgccaa cgccatagga gtacctacgg cgcttattta tggtgaaaaa
     3421 gctgaactgg attcaaatat taaagctttt cgtaaattgt gtattgctcc actagttaaa
     3481 aaacttcaag acgaattggt ggcaaaaata attagtcgtc aagagtacaa aaacggggaa
     3541 cgtatagaag ttaaaaaagt tctccaaaaa gatctattag agtatgctac tcaaattgat
     3601 aaaataacat ctggtggtgc gttttatatt gacgaagttc gtgatgaagt tgattatgat
     3661 gagttgccaa ataatgaagg aaaaattcgt attcgtacga aaaactatga agaaactgtg
     3721 aagggaggtg agaacgacaa tgccgaaagc taaaaaagta ccatttcagt ttactaacga
     3781 aattcagaat ggtaagcaca ttctcacctt gagtggaaat gtacagaaaa aatattggcg
     3841 tgatgatgat gtcataaacg ctaaagatat tcgtgactct ctagatactg taaccgatga
     3901 tattgtaatc aagttgaaca gcccaggcgg tgatgtgttt gaagggattg agatttacaa
     3961 tt
//
LOCUS       DASJOX010000178         3902 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJOX010000178 DASJOX010000000
VERSION     DASJOX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.182"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1080
                     /locus_tag="U4A12_002673"
     CDS             <1..1080
                     /locus_tag="U4A12_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEN1775002.1"
                     /translation="EKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIA
                     NIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSI
                     PLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAI
                     KEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLI
                     ANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVIC
                     SYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIIN
                     ISYIKQLCYRGRNIISMIKSREHKR"
     gene            1116..1832
                     /locus_tag="U4A12_002674"
     CDS             1116..1832
                     /locus_tag="U4A12_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1775003.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1851..3434
                     /locus_tag="U4A12_002675"
     CDS             1851..3434
                     /locus_tag="U4A12_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEN1775004.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3569..>3902
                     /locus_tag="U4A12_002676"
     CDS             3569..>3902
                     /locus_tag="U4A12_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775005.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGR"
BASE COUNT         1440 a          432 c          740 g         1290 t
ORIGIN      
        1 gaaaaatata tttttttgta ttatatatcc tatgtatgga atcaatttct aatacaattt
       61 gctaaagggt tagaaaaagt taaagctatg ggcatagcag gtattatcag cacaattgta
      121 acaataattg ccaatatttt cttcttgctt attctgaaat ggggattaat tggttttttc
      181 attgcaaata ttttatcaca gattatttca gctacttatc tgtcagtatt agtgagaata
      241 tgggaatata tcgattttga ggctaaagat cctcagctaa agagagaaat gctaaattac
      301 tcgattccat taattgcaac tgttgttgga tggtggatta atagtacagc tgataggtat
      361 gtggtttcct ttatagtagg tgtctcggcg aatggcttat tgtctgtggc atataaaata
      421 cctcaaataa ttaatacttt gcagggaata tttatacagg cctggcaaat ttctgcaatt
      481 aaagaatatg gagataatga gactgcaaaa ttttatggaa atacatttgt tatgattaat
      541 ttgttaatgt gcataacctg ttcaagtctt atcatattaa ctagaccgtt ttcacatatt
      601 ctttatgcga aagactttta tgaagcgtgg aaatatgttc catttttatt aatagctaat
      661 gttttaaatt gtgcatcagg tctgttagga cctatcttat cagcaaaaaa agattctaaa
      721 tccatgatgt ggtctgcaat cataggggca ttagttaatg tgattatgag tgtagtcctt
      781 gtttataaga taggggttca aggtgccaca atagccactg ttatatgttc atatgtaata
      841 tatgttgttc gtaaacgagc tgtgggaaaa gatattttta taaagcatta tcatattgtt
      901 gtggaatctt ggggattatt gtttgttcag gcaatcatag aaatatattt taaaaattat
      961 gtaatagaaa ttataattat gattatttta attattataa atatcagtta tattaagcag
     1021 ttgtgttata gaggaagaaa cattatttca atgattaaga gtcgagagca caaacggtag
     1081 acgatgttat ttttataaaa agatataatt tattaatggt cgataattat gaaaggaaaa
     1141 atattcttaa aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga
     1201 agttaaataa caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat
     1261 tgtgttatta tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct
     1321 attgtagtaa cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat
     1381 ctagttcctt agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag
     1441 aagtagaggc tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat
     1501 ctattgaaaa aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa
     1561 aaaaattgca agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata
     1621 ttcctagaac acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata
     1681 atcgcaatgt gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag
     1741 taggtgagat tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga
     1801 tttataatca tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg
     1861 tacagatttt agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat
     1921 ttgataatac aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat
     1981 gtgggtttct cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag
     2041 aagaattagc acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga
     2101 actatggcga atataatata gaggatcttc cagattatga tggttcatct attgatggaa
     2161 ttaggattgc gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa
     2221 agaaaaaagg ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag
     2281 agtttttagc agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg
     2341 atagttttgg aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata
     2401 atctagaaaa agatatacag ataggttttc actcacataa taacatgcag cttgcatatt
     2461 ctaatgcaca aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg
     2521 tgtacggaat gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga
     2581 atgataatgc aggaaaaaaa tatcaattaa aaccattatt atatataatt gatgaaatac
     2641 taaatagttt ctatcagaag agttattggg gatattcatt accaaactat atttcagcag
     2701 ttcataatgc tcatcctaat tatgcaagct atctgtcata taagaatact ttaacaattg
     2761 aagcaatgaa tgaaatattc gaaatgatgg atgaagaaaa aaaggtatct tatgataaac
     2821 aatatataga agatttatac tacaaatata tggaaactgg gaaaatacag gaggaatata
     2881 tggaagaact gagaaaattt ctctcagaca aaaaaattct actgattgca ccaggaaaaa
     2941 gttctgtaga tgaggtagaa agaataaaag agtttgttac caaagaagaa gtcgtaataa
     3001 ttagtgtaaa ttttgaatat tctcatatag atatagatta cgtattttta agtaatttaa
     3061 gacgttttca tgaattaccg aaagaaagca gaactaaatg tattgttaca tcaaatatac
     3121 aagcatcgga tgtatattta cggacgagtt ataaaaagtt gttatctgaa agggaagttg
     3181 taagagacaa tgcaggatta atggcaatta gatttttagc cgatatgaat gtggatgaaa
     3241 tattcttagc tggatttgat ggatattcac ataatgaaga tgagaattat ggtgagcaat
     3301 ccatggaaat tataacaaaa tttgcattat tagatgcaat gaacgtgcaa atgagagatg
     3361 aattgtcaga attagctaaa aaagtaaagg tgacattttt aacggatcca cgttgggttc
     3421 gaattgataa gtagtagtaa taatgttttt aaaattttga taaagagata gtgagagaat
     3481 aaaaatgggt aaagtcttaa ataaaaaaga agaaaaatat ttaagggacg atggggcgag
     3541 atgaaactct aattttgaac ttttaagaat catttctata ttgtgtattg ttgcttacca
     3601 tgctaatttg cgtttaaatt ataatgcaga cggaattata gtacatggtt cattaactgc
     3661 taaccagatt ttcttgaatt atattggtat ttggggtcag ctaggagtta attgttttat
     3721 atttatttcg gcatattttt taagtgagaa aagaaacatt cactttgaaa atgttttggg
     3781 aattgtatta gaatgtattt tttatagtgt attagtttat ggaattcttt gtgcatgtgg
     3841 aatagaaaca ttttcattga acagctttgt aaaagaaata ttatcatttg ctctaggtag
     3901 at
//
LOCUS       DASJOX010000179         3890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJOX010000179 DASJOX010000000
VERSION     DASJOX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.183"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..741
                     /locus_tag="U4A12_002677"
     CDS             55..741
                     /locus_tag="U4A12_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN1775006.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            743..1687
                     /locus_tag="U4A12_002678"
     CDS             743..1687
                     /locus_tag="U4A12_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN1775007.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1886..2530
                     /gene="pcp"
                     /locus_tag="U4A12_002679"
     CDS             1886..2530
                     /gene="pcp"
                     /locus_tag="U4A12_002679"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN1775008.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2609..3886
                     /locus_tag="U4A12_002680"
     CDS             2609..3886
                     /locus_tag="U4A12_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775009.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1242 a          675 c          861 g         1112 t
ORIGIN      
        1 tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa atatatggaa
       61 aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt taaattagac
      121 tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg tcttggcatt
      181 caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat ggcgattttt
      241 atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga atcagcggct
      301 tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat ttatgggatc
      361 tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg atttgttcgt
      421 ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga agtccatcct
      481 aagcatgaag aagaactaaa aggtatggct tcagctatgg aaaatgtctg ctggttcttt
      541 ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac actgaaagat
      601 ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt ggctatcacg
      661 gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc gaagaaatat
      721 tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt tattaggcga
      781 taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca ctggagtaag
      841 aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct ggacggtctt
      901 aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg gcttgatctt
      961 tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc ggcttccatc
     1021 aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta ttccagcttt
     1081 gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg ctttagtcgg
     1141 cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc gagataatcg
     1201 accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc cgttaagcat
     1261 gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag ttgggacagt
     1321 gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg gcatcatcat
     1381 ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg cggccatcac
     1441 cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga atccagcgtt
     1501 aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac cgatggctgc
     1561 taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg gagtgatcaa
     1621 aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata tgattctatt
     1681 taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc taacattgta
     1741 gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt atttaaacta
     1801 ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa ccatatgtta
     1861 aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg atccatttgg
     1921 tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg aaatcagcgg
     1981 tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc aagtagtcaa
     2041 agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag caggtgggcg
     2101 gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa tccctgataa
     2161 tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg cttactttac
     2221 ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac cagcgtacat
     2281 ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc ttcatttgat
     2341 cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct cacccgatca
     2401 agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg cttcttcttt
     2461 gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa atgccggaac
     2521 aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag tagccagctt
     2581 tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct ttgaagaatg
     2641 gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat acacaagcag
     2701 tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag tcgataagca
     2761 ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg caacagattg
     2821 ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc agcaaaaatt
     2881 gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc caaaaaccgg
     2941 aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct ctccgacagt
     3001 ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg aattattatg
     3061 gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact gttttgtctt
     3121 gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag ctcagcggct
     3181 cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag cattggaaaa
     3241 tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac agataaaaaa
     3301 agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat tcttgatcaa
     3361 ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag tgccagtaca
     3421 tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac gtcaacgaac
     3481 accagaagtc actgctcaga tggaatattt tgcgaaaaac caacgattca tccaaccaaa
     3541 aatctatcta tttatttgga agcaatattg tcgctatggt tttacagaag ggttcttcga
     3601 cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag aacgagaagc
     3661 tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc aactagtagt
     3721 gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag gaaaacatta
     3781 tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag aagaatcact
     3841 gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa
//
LOCUS       DASJOX010000180         3875 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJOX010000180 DASJOX010000000
VERSION     DASJOX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.184"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..2257
                     /locus_tag="U4A12_002681"
     CDS             158..2257
                     /locus_tag="U4A12_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1775010.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="U4A12_002682"
     CDS             2453..3316
                     /locus_tag="U4A12_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1775011.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="U4A12_002683"
     CDS             3421..>3875
                     /locus_tag="U4A12_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1775012.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DASJOX010000181         3873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJOX010000181 DASJOX010000000
VERSION     DASJOX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.185"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U4A12_002684"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U4A12_002684"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN1775013.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="U4A12_002685"
     CDS             complement(2261..2512)
                     /locus_tag="U4A12_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN1775014.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="U4A12_002686"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="U4A12_002686"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN1775015.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="U4A12_002687"
     CDS             3462..3680
                     /locus_tag="U4A12_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775016.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1074 a          820 c          717 g         1262 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tag
//
LOCUS       DASJOX010000182         3825 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJOX010000182 DASJOX010000000
VERSION     DASJOX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.186"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1256)
                     /locus_tag="U4A12_002688"
     CDS             complement(<1..1256)
                     /locus_tag="U4A12_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1775017.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="U4A12_002689"
     CDS             complement(1270..2487)
                     /locus_tag="U4A12_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1775018.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3825)
                     /locus_tag="U4A12_002690"
     CDS             complement(2477..>3825)
                     /locus_tag="U4A12_002690"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN1775019.1"
                     /translation="IVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYN
                     QFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQF
                     ASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHP
                     DNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMN
                     PPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVL
                     FRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASR
                     EFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFE
                     EEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1053 a          826 c          648 g         1298 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattg
//
LOCUS       DASJOX010000183         3789 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJOX010000183 DASJOX010000000
VERSION     DASJOX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.187"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1314
                     /locus_tag="U4A12_002691"
     CDS             <1..1314
                     /locus_tag="U4A12_002691"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN1775020.1"
                     /translation="EYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDL
                     QSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEF
                     YTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELN
                     TTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADT
                     TFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQ
                     KLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKL
                     SEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVG
                     NDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1304..2521
                     /locus_tag="U4A12_002692"
     CDS             1304..2521
                     /locus_tag="U4A12_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1775021.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2534..>3789
                     /locus_tag="U4A12_002693"
     CDS             2534..>3789
                     /locus_tag="U4A12_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1775022.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1308 a          651 c          791 g         1039 t
ORIGIN      
        1 gagtatcttt tcaacgtctt agcggatcaa gcaaaacaag caacgttcca attgaacgat
       61 ctaaacaaag cctttgtgca attggcatct acctacaatc aatttaatgg gttatttgat
      121 gatgtggatc tccaatcaaa aaaactagga acagatgagc aacaacgaaa tgtgacgatt
      181 accgaagtca tcaagaaatt gaatgatgta gatgttttag aacacgatgg ggacgtgatc
      241 ggggatgcgt atgagtttct gatcagtcaa tttgcttcag aagcagggaa gaaagccgga
      301 gaattttaca cacctcatat ggtttctgat atgatggcgc aaatcgtaac gctcgatcaa
      361 aaggaacgac gattctttag tgtttttgat ccaacgatgg gttcaggttc attgatgtta
      421 aacgtacgta actacttaac acacccagat aacgttaaat atcatgggca agaactaaat
      481 acaacgacat acaatctagc aaaaatgaac ttgattttac atggtgtcga cgccgaagaa
      541 atgaatctcc gcaatgggga tacgttgaat aaggactggc caacagatga accttacaca
      601 tttgatgcag tagtaatgaa cccaccttat tcggctaatt ggtcagcaga tacgactttc
      661 ttagatgatt ctcggtttaa tcgttatggg aaattagcgc caaaatccaa agcagatttt
      721 gcgtttcttc ttcatgggtt ctatcatttg aaggaaacag gtaccatggc catcgtctta
      781 ccacacggag ttttattccg tggcgctgca gaaggggtca tccgtcaaaa attactagaa
      841 gatggcagta tttatgctgt gatcggtatg cctgcgaact tgttctttgg gacatcgatt
      901 ccaacgacgg tgattgtgtt gaagaagaat cgtcaaactc gagatgtatt attcattgat
      961 gctagtcgtg aatttgttaa agggaaaaat caaaataaac tatcggaaga aaacattcaa
     1021 aaaatcctcg aaacctacgc tgaaagaaaa gatgtcgaga aatacgctca tttggcaacc
     1081 tttgatgaaa tcaaagaaaa tgactataac ttaaatatcc cacgctatgt agatactttc
     1141 gaagaagaag aaccaattga tatggttcat gtcggaaatg acatcaagaa gatacgacaa
     1201 gaacagcaag tgctagaaaa aaaactttta gaagctatct cttccctgca aacaacacct
     1261 gaaaatgaag cgtggttaca aggtgcgctg gaggtgttca aacatgagca atgatacaca
     1321 accagaaatc cgtttcccag gatttacaga tgattgggaa cagaggaagt tgggggactt
     1381 cgttgtagat tatgttgaaa agacaagtgt acaaaatcaa tttcctatgc taacttcatc
     1441 tcaacaaaaa gggatagttt tgcaagaaga ctattttgca aatcggcaag taactacaga
     1501 aaataatatt ggttactttg tactacctag aggatatttc acgttccgta gtcgaagtga
     1561 taatgatgtg tttgtgttta accgtaatga cattattgat agagggatta taagctattt
     1621 ttatcctgta tttactttaa aatctgctga ttccgacttc ttcttaagac gtattaacaa
     1681 tggaatccaa cgacaattat ctattcaagc agagggaaca ggtcaacatg ttctgtctct
     1741 aaaaaagttt aaaaatatag tagcaatgtt tcctagtgag gaagaacaac aaaaaatcgg
     1801 caccttcttc aaacaacttg acgacacgat cgctcttcat cagcgtaagt tagatttgct
     1861 gaaggaaacg aaaaaaggat tcttacaaaa aatgttccct gaaaacggag caaaagtgcc
     1921 agaaatccgg tttcctggtt ttacggaaga ttgggaagag cgtaagttgg gagaattagg
     1981 taaaactcaa tcgggaattg gatttcctga tgccgagcaa ggtggttctg aaggtactcc
     2041 attttttaaa gtttccgaca tgaataacat tggtaatgag tatgaaatga gaaatgcaaa
     2101 tcattatgta tctaatgagc aaattgagcg taaaaaatgg aagccaataa aagatgtgcc
     2161 agcgattata tttgctaaag ttggcgcagc aataatgctt aatagaaaac gtttggtcac
     2221 aagccctttc ttaatagata acaataccat ggcttattta tttaataata cttgggatat
     2281 ctattttggt aaaatattgt ttgaaactat aaatttgcca agatactccc aagtaggggc
     2341 attgccttct tataacagtt ctgatattga gaatatcagt gttaaggttc ctgtaaagga
     2401 tgaacaacaa aaaatcggta ctttcttcaa acagctagat gatacgatcg ctcttcatca
     2461 acgtaagtta gatttactaa aagaaacgaa aaaaagcttc ttacaaaaga tgtttgtcta
     2521 ggagggaaga agaatgaaaa ttagtcaccg tgaggaagca gaagtcgaag aacagttgat
     2581 tcgtgtactt ggagaagggc ataatcagtg gacgtatcgt ccggatctaa aatcagaaga
     2641 agacctttgg gtcaatttac gccaaaaaat tatctcaaat aatcaagcag aactgaatga
     2701 ttttcctttg accgataaag aatttgaaac gatcaaaacg gaattactgc tgcgaacgaa
     2761 aaccccattt gatgctgcga aatggttaaa aggtgaaaat gggatggctc ggatcacgat
     2821 tgaacgggaa gattcgcaat tgggttctgt ctcacttatc ctttattcca atcaagacat
     2881 tggtggagga atctctactt atgaagtggt ccatcaaatt gcaaaacgtg gaagtaacat
     2941 agaagcacgg gatcgacgtt ttgacgtcac cttattaatc aatggattac cgattgtcca
     3001 aatcgagctg aaacaagtaa ctgcaaaaga tggtgtttat caggcattta accaaatcaa
     3061 aaaatatgca gaagaaggta tgtttcggaa caatatcttt tcgaccttac agttgtttgt
     3121 ggtttccaat gaacaaacca ctcggtattt tgcaaatgcc ttaccgaaag acttgcaccc
     3181 taagttttta tttagttggc gaacgaaaga caatgaaaaa gtagaaaact tatatgagtt
     3241 ttgtaaacaa gtgttaaata tcccggatgc tcatcgtttg attgcggatt atacaatcgt
     3301 cagtgaagat caagacaata aaaccctcat ggtgttacat ccttatcaag tacatgcgat
     3361 tcaagccttg tttattgccg caaataaaca tcaatcaggg tatgtgtggc atgcgactgg
     3421 ttcaggaaag acgttgacta gttttgtatc tactaagctt ttagcacgaa aatcggggat
     3481 cgatcgaacg atcatgttag tggaccgcaa agatttagat aatcaaacca ccacagagtt
     3541 tacaaaattt gcttcggaat ttaatactgg gatttcttcc gggaacgcca aagcgaatag
     3601 cttgatcgtt ggaacgggaa gtgccaaaga attaagtgaa accctgttgg cagatgccaa
     3661 tgcaaatgtt gtaatcatta caacccgtca aaaactagat gcggcgttga aatatgcaaa
     3721 gaaacaagaa gagaaaaaag gaaccaatcg cttccaaaaa ttaatgggcc aacatatcgt
     3781 gtttgtagt
//
LOCUS       DASJOX010000184         3710 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJOX010000184 DASJOX010000000
VERSION     DASJOX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.188"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..489
                     /locus_tag="U4A12_002694"
                     /pseudo
     CDS             108..489
                     /locus_tag="U4A12_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            620..1570
                     /locus_tag="U4A12_002695"
     CDS             620..1570
                     /locus_tag="U4A12_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1775023.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2543
                     /locus_tag="U4A12_002696"
     CDS             2088..2543
                     /locus_tag="U4A12_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1775024.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2854..3210
                     /locus_tag="U4A12_002697"
                     /pseudo
     CDS             2854..3210
                     /locus_tag="U4A12_002697"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3259..3710
                     /locus_tag="U4A12_002698"
                     /pseudo
     CDS             3259..3710
                     /locus_tag="U4A12_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1321 a          579 c          652 g         1158 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac
//
LOCUS       DASJOX010000185         3669 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJOX010000185 DASJOX010000000
VERSION     DASJOX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.189"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..470
                     /locus_tag="U4A12_002699"
                     /pseudo
     CDS             75..470
                     /locus_tag="U4A12_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="U4A12_002700"
     CDS             complement(465..683)
                     /locus_tag="U4A12_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775025.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="U4A12_002701"
     CDS             790..1104
                     /locus_tag="U4A12_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1775026.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="U4A12_002702"
     CDS             1208..2386
                     /locus_tag="U4A12_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1775027.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="U4A12_002703"
     CDS             2789..3082
                     /locus_tag="U4A12_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN1775028.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1255 a          538 c          715 g         1161 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactcttt
//
LOCUS       DASJOX010000186         3645 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJOX010000186 DASJOX010000000
VERSION     DASJOX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.190"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..717)
                     /locus_tag="U4A12_002704"
     CDS             complement(133..717)
                     /locus_tag="U4A12_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1775029.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="U4A12_002705"
     CDS             complement(928..1131)
                     /locus_tag="U4A12_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775030.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="U4A12_002706"
     CDS             complement(1141..1563)
                     /locus_tag="U4A12_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775031.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="U4A12_002707"
     CDS             1801..2415
                     /locus_tag="U4A12_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1775032.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="U4A12_002708"
     CDS             complement(2580..3404)
                     /locus_tag="U4A12_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775033.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="U4A12_002709"
     CDS             complement(3428..3583)
                     /locus_tag="U4A12_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775034.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT         1209 a          636 c          518 g         1282 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatc
//
LOCUS       DASJOX010000187         3583 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJOX010000187 DASJOX010000000
VERSION     DASJOX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.191"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..1900
                     /gene="yfcC"
                     /locus_tag="U4A12_002710"
     CDS             161..1900
                     /gene="yfcC"
                     /locus_tag="U4A12_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1775035.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2057..3241
                     /gene="iadA"
                     /locus_tag="U4A12_002711"
     CDS             2057..3241
                     /gene="iadA"
                     /locus_tag="U4A12_002711"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN1775036.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3352..3555
                     /locus_tag="U4A12_002712"
     CDS             3352..3555
                     /locus_tag="U4A12_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1775037.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1152 a          594 c          828 g         1009 t
ORIGIN      
        1 gagaaaaaaa taatcataca taggagaaaa aaaacaaaat agaaaaaagt gattttctaa
       61 taaattattc atgtggaaat gtcattttta ctgtataatt catttgatta tccatttttt
      121 attcatcaaa attactgaaa aaagaaagga agaaataaat ttgaagaaaa caaattcttc
      181 aggaaaaccg aaacgcgact tcactaaatt atcgacaccc catacgtatg tcattatttt
      241 tggtgtagtc atctttgcat ggatactgac ttttgtcgtt ccagcaggaa agttcagcac
      301 gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt actcgtactg ttttacgtca
      361 agattccttc cgttatgctt acgagctgga taagtcgtat gtgtttgatc aattagaaga
      421 actgcaagat catccagcag aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa
      481 agtcattgct gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac
      541 agatgatgtg ctttacgatc agtacggcga aaatatctat gatacttcta aaaaattgca
      601 taaaacagcc aaaatatggg gaacagatga ttttggcggc tttggtttct taaattttgt
      661 ttttgaagga ctagtatctg gagataagta tggatctgct gtagggatcg ctgcattgat
      721 ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg acgcaggtat
      781 ctatgcgttt atcagtaaaa ctaagggact ggagcgttta gctttaccgc tattattctt
      841 tgctttttca ttcggtggcg ctacattcgg gatggcagaa gaggtaattc cattctcaat
      901 ggtgatggtt ccatttgtca ttgcgttagg atatgactcg atcgtagcag taaccgtgac
      961 ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg agcccgttta gcgtagcggt
     1021 ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg acattccgat tgattatgtg
     1081 ggtagttgtt actgcattag ccgctggtta tatgatgata tatgcagaaa aaatccgtaa
     1141 aaaaccagaa agttcgttga cttacaaatc agatgattat ttccgttctc atatcaaaaa
     1201 gacatctgat gaaaataaaa aattcatgct tgggcataaa ttgatcttat tagagatgtt
     1261 agttgtcctt gtctggatcg tttggggcgt gactcaaaaa ggctattaca ttccagaaat
     1321 cgcttctcaa ttctttgtga tgggattagt tgctggaatc attgctgttc ttttcaaatt
     1381 agatggaatg aaagtcaatg acatcgcttc ttctttccaa tctggtgcag ctgatttagc
     1441 tggtacagca atcgtagtag gtatggccaa agggattctt cttgtcttgg gaggatctga
     1501 tgcatctgtt ccttcagctt tgaatacgat tttgcatggg atcgggacgg cgttgaccgg
     1561 tgttccagct gtcattggtg cttgggcgat gtacatcttc caaagtttgt tcaacttggt
     1621 tgttacatca aattcaggac aagcagcttt gactatgcca atcatggctc cactggcaga
     1681 tttagtaggt gtatccagac aagtagcagt tcttgcttat cagcttggtt caggttttat
     1741 ggacgctttt acacccgtat cagccagctt gattggtgtg ctaggtgttg cacggatcga
     1801 atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac
     1861 tattttcatc atgattgcca ttgcaatagg tctgcagtag tactggagac ctgtaaataa
     1921 aaaagtaatc caaacagtag ataaggactc caagaaccgt cattttacgg taaaatcaaa
     1981 ggagtaaaga tcctatcgtt tggattattt tttaagatcc tatcatcagt tatacttaaa
     2041 gaaagaggaa aaatgtatga aaatcattcg acaaattgaa gtgtttgcac cagattactt
     2101 aggaaaaatg gatgtactga ttgccggtgg gaagattttg gctgtagaag aacagctaga
     2161 aggcggctac gaaggagtgg aagtagaaga actttcaggt gagggaaaag tactaacccc
     2221 gggtttcatt gactgtcatt tccatatttt aggtggagga ggagaaggcg gttatcaaaa
     2281 tcgaacgcca gaagtcacat tgagccaatt gactacagct ggtgtaacga cagttgtcgg
     2341 ttgtttggga acagacggag aaggtagaga tatgactgcg ttgatcagca aagcgaaagg
     2401 tctggaagca gaaggaatct ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa
     2461 aacagtaaca gattctatca tcaaagactt tttgacaatt gataaaatta tcggaatcgg
     2521 agaaatcgca gtatccgatc accgttcttc tcagccaagt tttgaagagt tcgctcgtgc
     2581 agtagcagat gctcgtgtag gaggcatgtt atccggaaaa gctgggatca tcaatgtcca
     2641 cttaggcgga ggaaaaagaa agctagagct attgacacga atcgtagaag aaacagaaat
     2701 tccgatcaca caatttttgc ctactcatgc aaatcgcaca ccagaattat ttgaagcttg
     2761 cgtggcattt gccaaacgtg gtggcacgat tgacttcact gctagcgaag atcctgattt
     2821 ttgggagaag acagatggtg aagttcgctt cagcaaagca ctgaaacgat tgatcgagga
     2881 agatgtttct ttagataatt tcacgatgac ctcagatgga caaggaagct tgccttattt
     2941 tgatgaaaac aatcatttcc tagggttagg agtaggaagt gccaaggcat tgctagtagg
     3001 gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa atcgccttac gagctataac
     3061 cagtaatcca gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga
     3121 tgcagatctg tgtatcttgg ataaagaaac gcttgatatt gatacagtta tcgctaaagg
     3181 agaaataatg gtccaagaaa aagaagtcaa agtatggggg actttcgaaa aaagttttta
     3241 atcaacagct ctaactatct tttttagaaa acgagcgttc tgtttgtctg tatctgttgt
     3301 tgatgttacc ctgaaggtag atcaataaac aaactaagag gaggaagcac aatggcagat
     3361 ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac
     3421 ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga aagcacaatc actaggtgct
     3481 gacatcaaag aaaaagctcg aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa
     3541 gacgaaaaaa aataaaaacc acttattact aattgaatca agc
//
LOCUS       DASJOX010000188         3453 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJOX010000188 DASJOX010000000
VERSION     DASJOX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.193"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U4A12_002713"
     CDS             complement(<1..2232)
                     /locus_tag="U4A12_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775038.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U4A12_002714"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U4A12_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          969 a          763 c          547 g         1174 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaa
//
LOCUS       DASJOX010000189         3404 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJOX010000189 DASJOX010000000
VERSION     DASJOX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.194"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..1193
                     /locus_tag="U4A12_002715"
     CDS             153..1193
                     /locus_tag="U4A12_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1775039.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2017..2349
                     /locus_tag="U4A12_002716"
     CDS             2017..2349
                     /locus_tag="U4A12_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN1775040.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2968..3309
                     /locus_tag="U4A12_002717"
     CDS             2968..3309
                     /locus_tag="U4A12_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN1775041.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1265 a          460 c          646 g         1033 t
ORIGIN      
        1 ggtagtacat aattcatttt atttctgatt ttaggtagta tttaattaaa aaacagtttt
       61 aatgcgtttg cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat
      121 aaataaaaca acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg
      181 atagagttta caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat
      241 acaaaaaaat gaaagattca actaagattg cctatatgct tttaaaagca agattagaga
      301 tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa
      361 atgaactatg tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt
      421 tggaatcctt tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa
      481 ataatcctaa tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac
      541 tcgaaaaatt tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca
      601 tacccaccct cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa
      661 tcataccgtg ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata
      721 ataatatatt agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc
      781 aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc
      841 ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa
      901 ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtggt atttcgatag
      961 tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt
     1021 taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg
     1081 tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac
     1141 gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta
     1201 ctgacaaagt gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct
     1261 aattatatta cgaatattac aaaataatag ctattttaat agaatttata agttaaaata
     1321 ttatgataaa aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat
     1381 ttcaaatata gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata
     1441 cgcgaagaga gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc
     1501 aggcaatacg aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt
     1561 gtgatgaatt gattttcgtt ctgcactttt ggatgataaa tttggacaat tgaaaataca
     1621 aaaaaaagaa aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg
     1681 gcattaagat ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg
     1741 atttagtata acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa
     1801 atacgtgata ttacgatgaa tctatgccta agcacaatgt tataaaaatg tccgtgtggc
     1861 tctttgccgt atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct
     1921 tttttgtttg tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt
     1981 gttaaaaata tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt
     2041 ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa
     2101 ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac
     2161 aaagtctcag aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat
     2221 cctatcccct taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta
     2281 ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg
     2341 atcggctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
     2401 attgtcgaga cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct
     2461 atcaacaatt cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat
     2521 tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata
     2581 agcgcagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta
     2641 atcatgagca gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg
     2701 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
     2761 acttgcgagg acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc
     2821 tcgtttcgga cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac
     2881 gataaaatat gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat
     2941 gaaatttacg taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc
     3001 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
     3061 agaggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat
     3121 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
     3181 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
     3241 tggtcatatt tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa
     3301 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagga gtcacgatga
     3361 ttaataaaaa ggtagtatta ccttaggcat tgagtaagga attt
//
LOCUS       DASJOX010000190         3228 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJOX010000190 DASJOX010000000
VERSION     DASJOX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.195"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..2165)
                     /locus_tag="U4A12_002718"
     CDS             complement(552..2165)
                     /locus_tag="U4A12_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1775042.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="U4A12_002719"
     CDS             complement(2306..3214)
                     /locus_tag="U4A12_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1775043.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          556 c          571 g         1063 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttt
//
LOCUS       DASJOX010000191         3221 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJOX010000191 DASJOX010000000
VERSION     DASJOX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.196"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4A12_002720"
     CDS             301..585
                     /locus_tag="U4A12_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775044.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4A12_002721"
     CDS             588..1004
                     /locus_tag="U4A12_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775045.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4A12_002722"
     CDS             complement(1141..1860)
                     /locus_tag="U4A12_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1775046.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="U4A12_002723"
     CDS             2535..2630
                     /locus_tag="U4A12_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1775047.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="U4A12_002724"
     CDS             complement(2932..3177)
                     /locus_tag="U4A12_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775048.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DASJOX010000192         3206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJOX010000192 DASJOX010000000
VERSION     DASJOX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.197"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..505)
                     /locus_tag="U4A12_002725"
     CDS             complement(8..505)
                     /locus_tag="U4A12_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN1775049.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(635..1345)
                     /gene="budA"
                     /locus_tag="U4A12_002726"
     CDS             complement(635..1345)
                     /gene="budA"
                     /locus_tag="U4A12_002726"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN1775050.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1358..3022)
                     /gene="alsS"
                     /locus_tag="U4A12_002727"
     CDS             complement(1358..3022)
                     /gene="alsS"
                     /locus_tag="U4A12_002727"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN1775051.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          898 a          705 c          527 g         1076 t
ORIGIN      
        1 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
       61 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
      121 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
      181 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
      241 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
      301 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
      361 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
      421 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
      481 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
      541 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
      601 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
      661 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
      721 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
      781 catgtccgcc aaatgtatgt gtatcatcga taaaatgcag gtgaaatccc gctgcagcaa
      841 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggata ttgctttgtt
      901 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
      961 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     1021 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     1081 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     1141 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     1201 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     1261 tatgagcaat cgtttctgtt ccatccatca agccggccat caaagctcct aatgtcccgt
     1321 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     1381 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     1441 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     1501 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     1561 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     1621 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     1681 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     1741 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     1801 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     1861 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     1921 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattttgctt
     1981 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     2041 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     2101 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     2161 agtttcttgc ttcatattcg attggatcat agccaattgc aataactaga tcacttcgtt
     2221 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     2281 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     2341 tttcaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     2401 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     2461 aagcagaacc taatttagga tcgctcaatg gcttgatgac attaccttgg acaggtgcat
     2521 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     2581 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     2641 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     2701 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     2761 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     2821 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     2881 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     2941 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     3001 cttgtttcat tttttcagcc attgaacatc gtccttttta tgctttattt ttaattatta
     3061 gttagaaata atcacaaata agtgatacag tttctaaacc tgtttcatac aaaagtatag
     3121 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     3181 tacataaaaa aaactgtcat tcttaa
//
LOCUS       DASJOX010000193         3052 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJOX010000193 DASJOX010000000
VERSION     DASJOX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.198"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..352)
                     /locus_tag="U4A12_002728"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="U4A12_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="U4A12_002729"
     CDS             complement(612..1160)
                     /locus_tag="U4A12_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775052.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="U4A12_002730"
     CDS             complement(1161..2018)
                     /locus_tag="U4A12_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1775053.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="U4A12_002731"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="U4A12_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="U4A12_002732"
     CDS             complement(2580..2909)
                     /locus_tag="U4A12_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1775054.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          892 a          620 c          384 g         1156 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct ta
//
LOCUS       DASJOX010000194         3042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJOX010000194 DASJOX010000000
VERSION     DASJOX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.199"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..1077
                     /locus_tag="U4A12_002733"
     CDS             202..1077
                     /locus_tag="U4A12_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775055.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="U4A12_002734"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="U4A12_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="U4A12_002735"
     CDS             complement(1642..2973)
                     /locus_tag="U4A12_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1775056.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1074 a          553 c          466 g          949 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aa
//
LOCUS       DASJOX010000195         3034 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJOX010000195 DASJOX010000000
VERSION     DASJOX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.200"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(251..865)
                     /locus_tag="U4A12_002736"
     CDS             complement(251..865)
                     /locus_tag="U4A12_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1775057.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1471..1848)
                     /locus_tag="U4A12_002737"
     CDS             complement(1471..1848)
                     /locus_tag="U4A12_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775058.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2148..3029)
                     /gene="galU"
                     /locus_tag="U4A12_002738"
     CDS             complement(2148..3029)
                     /gene="galU"
                     /locus_tag="U4A12_002738"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1775059.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          637 c          468 g         1011 t
ORIGIN      
        1 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
       61 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      121 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      181 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      241 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      301 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      361 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      421 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      481 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      541 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      601 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      661 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      721 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      781 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      841 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      901 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
      961 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1021 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1081 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1141 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1201 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1261 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1321 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1381 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1441 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1501 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1561 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1621 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1681 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1741 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1801 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     1861 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
     1921 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     1981 cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2041 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2101 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2161 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2221 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2281 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2341 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2401 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2461 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2521 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2581 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2641 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2701 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2761 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     2821 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     2881 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     2941 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3001 gcaggaatga ctgctttttt gaccttcact tgct
//
LOCUS       DASJOX010000196         3024 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJOX010000196 DASJOX010000000
VERSION     DASJOX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.201"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1243
                     /locus_tag="U4A12_002739"
     CDS             <1..1243
                     /locus_tag="U4A12_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1775060.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="U4A12_002740"
     CDS             1256..1888
                     /locus_tag="U4A12_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1775061.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="U4A12_002741"
     CDS             2093..2320
                     /locus_tag="U4A12_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN1775062.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="U4A12_002742"
     CDS             2389..2655
                     /locus_tag="U4A12_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1775063.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="U4A12_002743"
     CDS             2645..2914
                     /locus_tag="U4A12_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1775064.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DASJOX010000197         2980 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJOX010000197 DASJOX010000000
VERSION     DASJOX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.202"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1386
                     /locus_tag="U4A12_002744"
     CDS             1..1386
                     /locus_tag="U4A12_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775065.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
     gene            1373..>2980
                     /locus_tag="U4A12_002745"
     CDS             1373..>2980
                     /locus_tag="U4A12_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775066.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLF"
BASE COUNT         1011 a          442 c          508 g         1019 t
ORIGIN      
        1 atttgtacga ttctgattag tttttattat ggagaaaagt tttttaaaaa gatagatgct
       61 gcttttttat ttagttgttt atatactttt gcaacttatc gaacagttga tatctattat
      121 cgatccgcaa ttgcagaagc aatcgctctt acgattatcc cagctgtttt atattatgct
      181 tatggattga tattcaatag agaaaaaaaa gtcatcgctt tatctgtcag tataagtcta
      241 cttgtgtata ctcatgtctt aactacacta ttgtgtagtg cattgattgc ttgtatgatt
      301 atagttcgtt tgtttttaag caagcaaata aaaaacgata taaaatttgt ggtcgtgaat
      361 ttagtaaagg ctgttttttt gacttgtgca ttaactagct atttttggta tcctatgttg
      421 cagcagatga attatcaaga aatacacagt cccttcaaaa cagatttgca gctagaagca
      481 ctttctattt cagatagtat cattcaagcg ttaaataacg atattacaac atacactatg
      541 gggttattag gattggttgc gttgattttt cctttattca tactgaaaga aatggagaaa
      601 aaagaaaaaa tcatctatgt tggcgtagtt tctacatggg tatgcgtaac taatttattt
      661 ccatggtttt tgtttcaaaa aacaccgata agcttactcc agtttccttg gcgtatctta
      721 ggtatccaaa tctttttttc ttcaatgatc ataacgcttg tttttatgaa aaagactatg
      781 aataaaaaat attcatggtt aatcattggc gctactgtgg tgtttctact cgtcacgtct
      841 atagcagcaa aagaaaatta cgcaaaagtt atccaatcaa aacacggtca tattgttatc
      901 aatgaagaaa cactcccacg ttatacaaca tcagatatgg gaggattata cgattacgcc
      961 ccaacagaag cgataaaaga aaggcctcac ttagaaaaac atgaagtaaa agtaggagaa
     1021 tcgtgggaaa aagggaagta taaagctgca gataataaca ttacttacac agtggcaagc
     1081 aacaaaaagc aaaaagtgac agtaccagta tatgcttatt taggtacaca ggtaaaagta
     1141 aacggtaaag tcgtaaatga ctcttataaa aaacatggct tagtgaatgt ggatgtaaat
     1201 gctggagaaa ataagataga aattacaaca aaatatactc caactgctct catggcttta
     1261 tggatcagta ttctgactta ttgtttagtg gtgtattcaa gttttaaacg atttgcacct
     1321 aataagttga aagattctcg atatgtgact aaaaaggaga taaacaagaa gaatgaaaat
     1381 aaataaaaaa gtagtaatat tgagtggatt aattatagtt agtagtatat attctttcat
     1441 ctttttcaaa aatacacaga gtatttatac cactggagat ctttcttttc acttatctcg
     1501 aatcaagggg ttatcgacta tttttaccag tccgattaat tatgagacat tcaattatac
     1561 tggatatggt gtaaattatt tttatccctt tctgactttt tttccagctg taatgttata
     1621 ttggatgaca aaaaatttga ttgtaagcta tattatttac gtttggttat tgaacttgtg
     1681 tacaacactc gtagcttatc attatggcga gcggttttta aaacaaaaaa aagcggcatt
     1741 tttatttagt tgtctctata ttttctctgc ttatcggaca gtagacatct actatcgttc
     1801 cgcaattgcc gaagctatcg caataacact tgttatccct gtattatttt atgcttatca
     1861 aatcatctct ggaaaagaag aaaagtatcc gtctgtaaag ctagcattga gtatgagttt
     1921 gctagtgtac agccatgtac tatcaacatt aatgagtaca gcattaatta tcatttttat
     1981 ttttattcgt ttggtttcta aaggtttcaa gaatgcagat tttattgcta tatttaaaaa
     2041 attatttagt gcggcaggaa tgacattggt tctgacgagt gctttttggt atccgatgtt
     2101 tgaacaaatg ctctaccaaa aaatcaacaa acctagcgtg acaaatttat atgctcatgc
     2161 atctaatgtg ttcgatagtc tgactgaagc tatgaacaac gacttaacta cttactcaat
     2221 gggactagtt ggtctgttat ctttatgtat tcccctgatt ttatttaaaa aattaacaaa
     2281 catagaaaaa aagatttatt atggtacgtg tttgacttgg ctagctacaa ctagtttggt
     2341 gccatggtat cttctgcaaa atacgccagc caaattgtta caatttcctt ggcggatctt
     2401 aagcttgcaa ataatattct cttctctgat attaacgatg atatttttca aaaatagaag
     2461 atacaataag acaagagaac tattttattt atttatgact gttttgttat taatcacttt
     2521 aactgtttct agtaaaaaaa atttcaatca aaaaataatt tctcagccgg gacatattgt
     2581 tgtaaacaaa gaaacagtag aggcgtatac cactaaagga atggattatt ttttatatga
     2641 ttattctcct atcgcttcat tggagaataa ggagcatata ataaaacacg aagcaaagat
     2701 tggcagtcaa tgggtaaacc ttccttataa aacaactggt agttctatca tatatagagt
     2761 aactaatgag aagagccaag taatcacact tcctgtatat gcttatttag gaacgaatgt
     2821 agaaataaat catcaacctg taaaggagca ggtgaatgct aatggattgg tacaaataag
     2881 cggagtcaaa ggagttgctg atattaaagt aagcacaagt tatacagtaa cgactattat
     2941 ttcttatctt gttacattaa ctgcatattt atgtttattt
//
LOCUS       DASJOX010000198         2747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJOX010000198 DASJOX010000000
VERSION     DASJOX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.203"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            177..359
                     /locus_tag="U4A12_002746"
     CDS             177..359
                     /locus_tag="U4A12_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1775067.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="U4A12_002747"
     CDS             462..746
                     /locus_tag="U4A12_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775068.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="U4A12_002748"
     CDS             762..998
                     /locus_tag="U4A12_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775069.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="U4A12_002749"
     CDS             1020..1274
                     /locus_tag="U4A12_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775070.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="U4A12_002750"
     CDS             1356..1541
                     /locus_tag="U4A12_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEN1775071.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="U4A12_002751"
     CDS             1608..2003
                     /locus_tag="U4A12_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1775072.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="U4A12_002752"
     CDS             1993..2370
                     /locus_tag="U4A12_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775073.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..>2747
                     /locus_tag="U4A12_002753"
     CDS             2392..>2747
                     /locus_tag="U4A12_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775074.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDK"
BASE COUNT          963 a          465 c          486 g          833 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acgctgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaa
//
LOCUS       DASJOX010000199         2731 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJOX010000199 DASJOX010000000
VERSION     DASJOX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.204"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            175..519
                     /locus_tag="U4A12_002754"
     CDS             175..519
                     /locus_tag="U4A12_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN1775075.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="U4A12_002755"
     CDS             520..939
                     /locus_tag="U4A12_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN1775076.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="U4A12_002756"
     CDS             complement(1031..1381)
                     /locus_tag="U4A12_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775077.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="U4A12_002757"
     CDS             complement(1347..2567)
                     /locus_tag="U4A12_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN1775078.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1013 a          491 c          528 g          699 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg a
//
LOCUS       DASJOX010000200         2662 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJOX010000200 DASJOX010000000
VERSION     DASJOX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.205"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            634..888
                     /locus_tag="U4A12_002758"
                     /pseudo
     CDS             634..888
                     /locus_tag="U4A12_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            958..1470
                     /locus_tag="U4A12_002759"
     CDS             958..1470
                     /locus_tag="U4A12_002759"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1775079.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1467..2504
                     /locus_tag="U4A12_002760"
     CDS             1467..2504
                     /locus_tag="U4A12_002760"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1775080.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          974 a          469 c          534 g          685 t
ORIGIN      
        1 cttaaaatgc aaaataaatg caaataaggg ggtaactcct tatgaaaaaa caactaatag
       61 tattataaca cgtttttatg ccgagaaaac ttattggttg gaatgggcta tgtgttagct
      121 aacttgttag cgagttggtt ggacttgaat tgggattaat cccaagaaag taccaactca
      181 acaacacata aagccctgta ggttccgacc aataaggaaa ttggaataaa gcaacgaaag
      241 gagttgagta aaatgagtga gaaaacgaag aaaatcattc gagtatcatt agctttttca
      301 caagtagtag tgcaatcatt gatagtttat tatacttacc agcaatataa attatctaaa
      361 gagaattaag ttttgattat gtaataagtt ttggtactaa aaaagacttg atctaattag
      421 accaagtctt ttgttagtgt tatattaata acaaaataaa aataagaagt cgctcactcc
      481 ccgaccaaag tttgtgagca aaataaacag tcgatgtttg tttcttaatt gtatatcttg
      541 atattaaacg atttaagatt attcttcaag atatatattc gcaaatgtaa ttggaaaagc
      601 gacttcttaa acaaaattaa ggagtcgctt tttatggcaa aaataacgaa aaatgagcaa
      661 gaaaatttgg tggaaaatta cacaaaaaaa ccggcgtact ctaatagccg gttagacgca
      721 catttcgtgt gcacttctaa tccgaaattg agctttgacg ctatgacgat agtcggaaac
      781 cttaataaaa acagcgctaa aaagctatcc gattttatga gtttagatcc acaaattaga
      841 ctttgggata tacttcaaac aaaatttaaa gctaaagcac tacaagaccc cctatgttag
      901 gctagttgtc gtataggata ataagttata ataaagacta tacggaggat gattaccatg
      961 acaaataaaa acaatgaacg ttactctacc gaactgaaag cgacagccat caagaaaatg
     1021 atgccacccg aaaatagggg gattcgccaa ttaagtcaaa ctttgggcat tccagaagcg
     1081 actctttatt cttggcgtaa aaaagctaga attcaaggga atactacacc cggaacgaaa
     1141 aaaaatgtcg aaaaatggtc aagtgaagat aaatttttaa ttgtgatgga atcattctca
     1201 atgaaccaag tagaacttgc ggaatattgc cgaaagaagg gcttatttaa agaacaaatt
     1261 gaggcttgga agaaaacgtg cttatctgcg aatgaacagg aagaaaatcg aacgagagaa
     1321 cttgcaactg aactaaaaga agagaaaaaa caagcgcgtc aattagagaa agaattacgt
     1381 cataaggaaa aagcattagc ggaagccgct gctctcttat tactaagaaa aaaagcacac
     1441 gcgatttggg gggaccaaga ggaagaatga tttcatcttc agatcgcaaa caagctgttg
     1501 aacttattca agaagccaat cgcaatgggg cccgtctcac ctatgcctgt aatgagctga
     1561 atattagcgt tcgaacctac gaacgatgga cgcgtgaagg tacgatagcg catgatcaac
     1621 gccctttggc gaaacgtcct gtacccaaaa ataaactgac ggatcaagaa cgcgaaaaaa
     1681 taatagaaac cgtttcaaaa aaagaattta tgaatttgcc acctagccaa attgtaccaa
     1741 aacttgcgga ttgctcgatt tatattgcat cagagtctag cttctatcgt gtattgcgag
     1801 aaaaaaatat gcaacatcac cgtggccgaa gtcaagtttc acaaaaacgc ataccgccaa
     1861 gtcaccttgc gacgaagcca aatgaagttt ggacttggga tattacttgg ctaaaaggtc
     1921 caataaaagg actcttttac cggttatatc tcatcattga tttatttagc cgtaaaatgg
     1981 taggctggga gatttgggaa tcagaagaag ctcgtcatgc cgaaaaactt attaaacaga
     2041 cggtgcttaa ggaacgtatc caaggacagc cgttggtttt acactccgat aatggcagcc
     2101 ctatgaaagc cgcgactttt caagtcttac tcgaaaaatt aggaattcaa agttcttatt
     2161 caagaccacg agtgagtaat gacaatcctt attcggaagc ggctttccga acgttaaaat
     2221 atcgaccaga atatcccaat aacggcttta aaacaataga tgatgctcga aaatggacga
     2281 aagagtttgt cgattggtat aacgattctc atttgcatag tgggattcaa tttgtgtctc
     2341 ctgcagcgtg tcatgaagga cgacatctcg ctattttaga gaaacgaaag caggtttacg
     2401 aggacgctaa aaagcgccat cctgaacgct ggtcaaaagg tgttcgaaat tggactccac
     2461 atcaatcggt ggcgttgaat cctttaaaag aaacagaaag ttaacaggag cataatcttt
     2521 caagattatc aaataaccct cgtcacccaa taggggaaaa agttattctt cccctattgg
     2581 ataacgaggc caaccgacca tagggaggtt ctgctttttg ataggtggaa aataaaaata
     2641 cgacaactat attgacaaac ac
//
LOCUS       DASJOX010000201         2620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJOX010000201 DASJOX010000000
VERSION     DASJOX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.206"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..581
                     /locus_tag="U4A12_002761"
     CDS             <1..581
                     /locus_tag="U4A12_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN1775081.1"
                     /translation="KEKYHLKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQ
                     PIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGII
                     EAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIY
                     GDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            554..1231
                     /locus_tag="U4A12_002762"
     CDS             554..1231
                     /locus_tag="U4A12_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1775082.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1221..2363
                     /locus_tag="U4A12_002763"
     CDS             1221..2363
                     /locus_tag="U4A12_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN1775083.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2376..>2620
                     /locus_tag="U4A12_002764"
     CDS             2376..>2620
                     /locus_tag="U4A12_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1775084.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEA"
BASE COUNT          983 a          343 c          509 g          785 t
ORIGIN      
        1 taaaagaaaa atatcatcta aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc
       61 cagttgtaac cgaactagaa aatatagaaa aacaaatttt agctttgatc aattcaatgg
      121 aacaacaatc tcaaccaatt atttgcattt tacctaatag tgatgctggt agcgaaatta
      181 tcagaaaaat gtaccagaaa tatttaaaaa cggatattat ttatattacg aatttagagc
      241 cggaggaata cctgactata ttaaaagagt gcaaatttat gatcggaaat tcttcttctg
      301 ggataattga agctgcatca ttcaaaatac cagtaataaa tttaggaacc agacaaaatg
      361 gacgagaaca atctaaaaat attataaatg caaatctaaa tgaaaatgaa attgaaactg
      421 ctttgaataa aatacaagat cctcagttta taaaaagctt gaaaaatgtt aagaacatct
      481 acggtgacgg caacagttct agaagaataa caaaaacatt gaatcaaata ttgaatgatg
      541 gggaagatat taagtggatt caaaaacaaa tagcttatta aaatatacta gtgatccata
      601 cattattatt ggggctggcg gacacgcaaa agtcattatt gaaaccttaa gattagaaaa
      661 taagttaata tatggatttt ttgatgataa gcaaaataaa atgcaagatc tgaaatatct
      721 tgggaaaata aacgaattga atagctgggc ggatagcaaa aaaaaaatct ataaatttat
      781 tgtagctatt ggtgactgtg tagtaagaaa aaagattata aaaaaattga atatgccaag
      841 tgagcaatat ggaatagctg ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg
      901 ttatggaact gttatttttg cgaatgctgt aattaaccca gatgctgtaa ttggagagca
      961 tgctataata aacacaggta gcattgttga gcatgattgt agaattaata actatgttca
     1021 tttatctcca ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg
     1081 aattggttct caatgtatcc agatgaagaa aattggatct tggtgtatga taggcgcagg
     1141 gagcactatt gttaaggata ttccctcaca ttccttagct tatggcaatc cagctaaaat
     1201 taagaaagaa ggaattaatt ttgaattcta gaattttatt atcaagtcct catatgggag
     1261 gaacagaaga aaaatatgtt gctaaggctt ttaaagataa ttggatagcg ccattaggtg
     1321 aaaatgtgga taaatttgaa gaaagtatta caaaatatac tggttctaag ggcggagctt
     1381 ctgcgttaag ttcaggtaca gctgccatac atcttgcctt aagactgctt aatgttggaa
     1441 aaaacgatat tgtctttgct ccttcattta cttttatagc tacagtaaat cctattttat
     1501 atcaaggggc tgtaccagtg ttcattgact cagaacctaa tacatggaat atgagccctg
     1561 ttgcactaga gagcgcatta gaagaagctg aaagaaacaa ccaattacct aaggcaatca
     1621 tcgtagtgaa tttatatggt caaatggctg attatgaacg aattttagct attgcaaacc
     1681 aatttaatgt tccagtgata gaagatgcag cggaatcgtt aggttctact tataagggaa
     1741 tttcgtcagg cttatttggg acaattggga tctattcatt taacggtaat aagatcatca
     1801 caacctcagg tggaggagct ctagtgtcta gtgataaaaa atatgttgag cattcaagat
     1861 ttttagcaac acaagcaaga gatcaagcac cgtattatca acatagcgaa ataggttata
     1921 attaccgtct aagcaatatt tctgccggta tcggacgtgg acaaatggaa gtattgaatg
     1981 atagagtagc taaacgaaga gaaattttca atcgatataa ggaagctttt tctggaattg
     2041 atagaattga aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt
     2101 taacgatcaa tgattataat tctattgatc aagtagttaa cgtaataaat aaactaaatg
     2161 aaaaaaacat tgaaacaaga gctttgtgga aaccactaca tttgcaacca gtatttcaag
     2221 gggtaaaaat gtattaccat gataataatc ttagtgtgtc agaaaagtta tttaatagcg
     2281 gcttatgctt accttctgga agtaatatga cagatagtga acaagaattt gttattgatg
     2341 agtttaaaaa aatattcaag taggtgtgat attttatgaa cataggaatt attggtttag
     2401 gttccatggg gaaaagaaga cttagactca ttcaagaaca ctttaatgat attttactat
     2461 gtggtgttga tactaatgag gcgagacgaa aggaagttga aaaggaattt ggaataccaa
     2521 cttataatag tctaacagag gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta
     2581 catcccctat aacccatgaa tttattataa aagaggcttt
//
LOCUS       DASJOX010000202         2413 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJOX010000202 DASJOX010000000
VERSION     DASJOX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.208"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(248..469)
                     /locus_tag="U4A12_002765"
     CDS             complement(248..469)
                     /locus_tag="U4A12_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775085.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="U4A12_002766"
     CDS             complement(567..812)
                     /locus_tag="U4A12_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775086.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="U4A12_002767"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="U4A12_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..>2413)
                     /locus_tag="U4A12_002768"
     CDS             complement(2036..>2413)
                     /locus_tag="U4A12_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1775087.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          846 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tgg
//
LOCUS       DASJOX010000203         2407 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJOX010000203 DASJOX010000000
VERSION     DASJOX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.209"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1150
                     /locus_tag="U4A12_002769"
     CDS             5..1150
                     /locus_tag="U4A12_002769"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN1775088.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="U4A12_002770"
     CDS             1321..1779
                     /locus_tag="U4A12_002770"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN1775089.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="U4A12_002771"
     CDS             1938..>2407
                     /locus_tag="U4A12_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN1775090.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DASJOX010000204         2398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJOX010000204 DASJOX010000000
VERSION     DASJOX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.210"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U4A12_002772"
     CDS             207..2069
                     /locus_tag="U4A12_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1775091.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="U4A12_002773"
     CDS             2069..>2398
                     /locus_tag="U4A12_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1775092.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DASJOX010000205         2392 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJOX010000205 DASJOX010000000
VERSION     DASJOX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.211"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..238)
                     /locus_tag="U4A12_002774"
     CDS             complement(<1..238)
                     /locus_tag="U4A12_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1775093.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLF"
     gene            457..1827
                     /locus_tag="U4A12_002775"
     CDS             457..1827
                     /locus_tag="U4A12_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEN1775094.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1848..2132
                     /locus_tag="U4A12_002776"
     CDS             1848..2132
                     /locus_tag="U4A12_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775095.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          812 a          356 c          495 g          729 t
ORIGIN      
        1 gaaataagac ggaacaaacc gctctatacg tggctctttg gctagtatag ccattaatta
       61 tgccccatat aacacaagca ataatcactg tatcgcgttg cttcagttga tcagtatttc
      121 ttcggttttt aatcttatct ggtacacaat tgtggtatat gttagaaaca gttctcataa
      181 tttccttgaa tgttataaaa gtatctgtat aatgttcagt atattttgat gaacgcatat
      241 gtagtcctca tttcttggga gttttgagac tactatatgc gttttttgtt gatttaccaa
      301 catgtttaga cattttcctc taggaaaaaa ttaactagca ccacgcgtat tgtttatatt
      361 aaattagatg accgtgataa tgcacagcaa atttttgaaa gtatcaattc taccggtgaa
      421 agattgactg cctcggattt gattcgaaac ttcatcatga tggataaatc taatgaagag
      481 cagactacgc tttatactaa atattggcgt aggttggaag aagtctttga taattctaaa
      541 ggaatggaag actttttcag atactacctt gctgcaataa ctggggagta ttcagcaaag
      601 catattcttt atcaagcatt caaagactat tggcataaag agcgcgatgt atcaagtgat
      661 gccgaattgc tccaaaaatt agtgcgatat gctggttatt ttgcgagact ttactataac
      721 aaacctgaag gaaaatactc agaagtattg agcgatttcc aaagtatgga aagtatgatg
      781 cctgcacctt ttgttttggg attatctgaa tggtactatc atgaccaatt tatcactgaa
      841 gaccaatata ttgatgcgat taaggtagtg aatgattatc agcttaggag atattttaat
      901 ggggatgata caagcagagt gtcaaaagca tttccttcat atttgaaaag tgttaggaag
      961 tacgctaaag ctaatggata tgaaaatatc gttgatattg taatttatgt tcttgttact
     1021 agaaatcaat caaataatat ggcacttcca tctgacaagg cattgaaatc taatctattg
     1081 aatgccaatg cttatgcgat gagattggcc cgttggttac ttgaaaaaat agagaatcga
     1141 gaaaattcag caacacttga tatgagtaac ttgtccattg aacacatcat gccacagact
     1201 agtacttcat attgggaaga gaaggctggt acatcggggg aagaatacac aggattagta
     1261 aataccattg gtaatttaac actcgttaca aatcctgata attcggccgc gggaaataaa
     1321 gactttgaga ccaaaaagaa aatctttgaa gatacccttc atattcgtat gaataaagat
     1381 ttgtacgaat tgactgagtg gacatcatcg gatatttctg ccagaagtga agctctaatc
     1441 aatgaactga ttactatgta tccctatttg agatcgtcgg gcgactatga acacgatggg
     1501 aatagggaaa ttttcttaga ggcgcaagga ataaaagcaa ctgggtattt gaacgaagac
     1561 gagaccgtga ttatacattc aggttcagaa atttattcta agattaaaga tattgcatct
     1621 gattcgctag atgaaactcg ccaagagctg ttggataatg gaatcattga agaaacaatt
     1681 gggggactac aatttgttca agattacact gcctcatctg tttcgaatgc agcagctctg
     1741 ctattagggg gtagccggaa tggttgggac tactggaaat atgataacgg aataagtatt
     1801 aatgattcgc taagaaataa gaaatagagc atagaggtgg tgttaggttg gataagaaca
     1861 aactaagata tgcaattctt aaattgcgtg atgaaaaaaa gaatcctttt attgagctta
     1921 aagggcaagt tccagaagaa gatattttag aacaaactaa atttctttcg cgcaatggct
     1981 tactagaatc catggtttgg gcggataaca ctgttcatat ctgggggagt gtttctctgg
     2041 aaggggaaaa atatttagtt gaaaactcag gtcttgctaa ggcgtatgca ttggcaaaag
     2101 aagttaaaaa ttggattcca ttttggggct aagataagtt tttaaacgga gaaggtagca
     2161 aatcataatt ggtttgttgc cttttttgat tgcccaaaaa gaaaaatgac aaagtataga
     2221 gataaatgac atagtacacg ggaaaaatga cattgtatca ccgacaacgt ctaaatagat
     2281 gttgatatat atacgttccg tacaaataat tataaaaatt taaatgattt gagtagaagt
     2341 atcagtgaat atctcaaggg gaatagctat ctcaagattc gattttcaca ta
//
LOCUS       DASJOX010000206         2361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJOX010000206 DASJOX010000000
VERSION     DASJOX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.212"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            163..513
                     /locus_tag="U4A12_002777"
     CDS             163..513
                     /locus_tag="U4A12_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1775096.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            927..1745
                     /locus_tag="U4A12_002778"
     CDS             927..1745
                     /locus_tag="U4A12_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1775097.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1818..2177)
                     /locus_tag="U4A12_002779"
     CDS             complement(1818..2177)
                     /locus_tag="U4A12_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1775098.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          777 a          344 c          450 g          790 t
ORIGIN      
        1 aagctgcttc gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac
       61 aagctcgttt ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta
      121 gcgggtatta caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata
      181 caaacggcgt tttataaagc aatcattcct gagaacttag atgaggttaa actttattta
      241 tgggaagata acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg
      301 ctatttcttg atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg
      361 attgaaactg aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat
      421 ttttatttaa gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa
      481 ccatacccta taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga
      541 acatgaataa aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag
      601 atcaaatttg tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac
      661 tagctgacca aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct
      721 tatgtcaaca ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt
      781 aatacaaaaa atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt
      841 tttaacggag taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag
      901 atatcaaagg cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag
      961 cagtagatat ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca
     1021 aggctgtttt atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc
     1081 aatactacca attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg
     1141 aaaatggtgc ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa
     1201 aagcgatcga ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt
     1261 gtggtctgaa tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa
     1321 ccaatatcta ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa
     1381 ttttaaaatt tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc
     1441 gtgaaaaatt agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga
     1501 ttctttctga gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta
     1561 atcctgacca gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt
     1621 gtggtctgag ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc
     1681 aatgtccttc aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca
     1741 cgtaaacact aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa
     1801 ctatatttag aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta
     1861 tctaaaatac cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat
     1921 actaaatgcc cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg
     1981 caaggtattg ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga
     2041 tcaccaatca tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt
     2101 aacaagaatc catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa
     2161 aataaattat cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt
     2221 atttaacaca cctattcccc cttaactata actcaattct atataaaaaa tattctattc
     2281 tcaaataatt gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata
     2341 tcaagagcga ttaacgctgt a
//
LOCUS       DASJOX010000207         2348 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJOX010000207 DASJOX010000000
VERSION     DASJOX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.213"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..556
                     /locus_tag="U4A12_002780"
     CDS             <1..556
                     /locus_tag="U4A12_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1775099.1"
                     /translation="NIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKS
                     MGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIH
                     LDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFEN
                     VVTGKENWNDLYHANEVLKIAEEK"
     gene            557..1309
                     /locus_tag="U4A12_002781"
     CDS             557..1309
                     /locus_tag="U4A12_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1775100.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1314..2261
                     /locus_tag="U4A12_002782"
     CDS             1314..2261
                     /locus_tag="U4A12_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1775101.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          832 a          301 c          454 g          761 t
ORIGIN      
        1 aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc
       61 tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa ataattattc
      121 tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat tggattatat
      181 tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat attcgaatct
      241 tgaaattaac agtaatgatt tagcaacata tatattgaga tacgatgata aaattataga
      301 aattcattta gattattttg gaagagttcc tacaagaaaa tttcaattgt ttactttaga
      361 tgaatgtatc aatgttgact tattaaataa taaaataagt tttgaagaaa acattgttaa
      421 agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt tctttgaaaa
      481 cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag tattgaaaat
      541 agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg ggtctaaggg
      601 gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt tcgctttagc
      661 aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt gtttgaatac
      721 ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga aaattgtaga
      781 acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac gtgattcatt
      841 cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag atttgacttc
      901 acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa aaaatttaga
      961 taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta atatggtaaa
     1021 aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta ggcaacagac
     1081 acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa
     1141 aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt
     1201 agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga
     1261 agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct
     1321 gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat catttaggag
     1381 aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata
     1441 tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga
     1501 tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag
     1561 attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt
     1621 tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg
     1681 ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga
     1741 cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat
     1801 atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga
     1861 attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag
     1921 gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata
     1981 tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac
     2041 aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg
     2101 aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg
     2161 actctctagc agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt
     2221 taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt
     2281 agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg
     2341 ggatagaa
//
LOCUS       DASJOX010000208         2299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJOX010000208 DASJOX010000000
VERSION     DASJOX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.214"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(205..1104)
                     /locus_tag="U4A12_002783"
     CDS             complement(205..1104)
                     /locus_tag="U4A12_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN1775102.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="U4A12_002784"
     CDS             complement(1114..1470)
                     /locus_tag="U4A12_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1775103.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="U4A12_002785"
     CDS             1825..2121
                     /locus_tag="U4A12_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN1775104.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          717 a          436 c          399 g          747 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacat
//
LOCUS       DASJOX010000209         2293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJOX010000209 DASJOX010000000
VERSION     DASJOX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.215"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..750
                     /locus_tag="U4A12_002786"
     CDS             <1..750
                     /locus_tag="U4A12_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1775105.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          833 a          302 c          463 g          695 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     2221 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     2281 caaaggagtg taa
//
LOCUS       DASJOX010000210         2256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJOX010000210 DASJOX010000000
VERSION     DASJOX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.216"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            106..>2256
                     /gene="acm"
                     /locus_tag="U4A12_002787"
     CDS             106..>2256
                     /gene="acm"
                     /locus_tag="U4A12_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN1775106.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQK"
BASE COUNT          884 a          348 c          447 g          577 t
ORIGIN      
        1 tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg
       61 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
      121 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      181 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      241 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      301 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      361 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      421 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      481 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      541 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      601 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      661 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      721 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      781 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      841 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      901 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      961 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
     1021 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1081 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1141 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1201 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1261 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1321 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1381 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1441 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1501 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1561 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1621 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1681 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1741 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1801 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1861 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1921 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1981 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     2041 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2101 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2161 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2221 atagtaacta tctgtgctgg gatattctta caaaaa
//
LOCUS       DASJOX010000211         2233 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJOX010000211 DASJOX010000000
VERSION     DASJOX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.217"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..544
                     /locus_tag="U4A12_002788"
     CDS             <1..544
                     /locus_tag="U4A12_002788"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1775107.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="U4A12_002789"
     CDS             complement(900..1472)
                     /locus_tag="U4A12_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1775108.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPRDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="U4A12_002790"
     CDS             complement(1488..2093)
                     /locus_tag="U4A12_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN1775109.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          741 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc taggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DASJOX010000212         2181 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJOX010000212 DASJOX010000000
VERSION     DASJOX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.218"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1012
                     /locus_tag="U4A12_002791"
     CDS             <1..1012
                     /locus_tag="U4A12_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775110.1"
                     /translation="QNFYRESKGDLFIYSEFENARRYSERIKELGLFNDVVFVNHRKI
                     GSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTKILFSNVYKKNPDVKLNYIED
                     GLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKI
                     DENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKET
                     LIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTI
                     NSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYG
                     II"
     gene            999..>2181
                     /locus_tag="U4A12_002792"
     CDS             999..>2181
                     /locus_tag="U4A12_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1775111.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VF"
BASE COUNT          747 a          274 c          344 g          816 t
ORIGIN      
        1 tcaaaatttt taccgagaat caaaaggaga tttatttatt tattctgaat ttgaaaacgc
       61 tagaagatat agtgagcgta taaaagaatt agggctattt aatgatgttg tttttgtgaa
      121 tcaccggaaa atcggttctt cctttaaacg taaaattaac ttgttaacta accataatca
      181 atttttctcg gatgatttat ccgtttatga tgaaattttt atacaggggg aaaattattt
      241 cacaaaaata cttttctcca atgtttacaa aaaaaatcca gatgtaaaac taaattatat
      301 agaagatggt ttgggtactt atttgaagaa gagtatattt tcaacaagta aaaacgttca
      361 aagagttttt aaaatattaa ataaaaatag catcttcaaa aaaaaaattg acgattattt
      421 tgtctattgt ccggaattgt tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat
      481 agatgaaaac agtaaattga tgaaaaacat acaatcaatc tttgggatag aagaagtttt
      541 tgtcggagat aaatgtatct tttttgatca acctttatta tctgatggtt atggcataga
      601 tgaaacagat attttcgcta aagtaaatag tatattttct ggaaaaaaag aaactttaat
      661 taagcttcat cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat tgaattccag
      721 tttaccgttt gaagtcttgc tacttgaaaa tgacttctct tcaacacttt tgattagtcc
      781 tatatctaca atttcttttt caccatctat gatgtttgaa aagacgatca actctattat
      841 gttggctaaa ttaattttaa atgaatatac agcattatct gaagataaaa aagaaacatt
      901 gacaaatatt gttcttttct gtgaacatta taaccgtcta tcagaagaaa aaattttact
      961 cccagaaaac tgggcagaat taagaaagtt ggtagagtat gggattattt aacacacttt
     1021 ccaagtcaaa attagttaag tccagtttct ggtatactat agggaatttt tttattaagg
     1081 gaatttcatt cataactatt cctatttttg taaatattac tactcaagca gaatatggtt
     1141 tgattaataa tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa
     1201 attcggcaat taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat
     1261 cggtactttc tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt
     1321 ttttgttttc aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac
     1381 aaagttttgc tagttttgta attagttata tccagtctta ttttacgatt aactttaacc
     1441 attttaaata tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag
     1501 ctttgatgca gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac
     1561 tgtcatttgt gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt
     1621 tttttgataa aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt
     1681 ccttagcaaa tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc
     1741 ctgctgcggg gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat
     1801 ggatgtctac taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg
     1861 aaaaaaaagt gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga
     1921 ttgtaatggt agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg
     1981 ggttatcttt agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac
     2041 cagttaatgc tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa
     2101 gtgctatagt taatatacta ttaaacatga ttttcatacc taggttagga ataattgctg
     2161 ctggatacac aaccgtattt a
//
LOCUS       DASJOX010000213         2177 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJOX010000213 DASJOX010000000
VERSION     DASJOX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.219"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U4A12_002793"
     CDS             complement(<1..914)
                     /locus_tag="U4A12_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN1775112.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U4A12_002794"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U4A12_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN1775113.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          739 a          384 c          417 g          637 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaa
//
LOCUS       DASJOX010000214         2138 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJOX010000214 DASJOX010000000
VERSION     DASJOX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.220"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1987
                     /locus_tag="U4A12_002795"
     CDS             <1..1987
                     /locus_tag="U4A12_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN1775114.1"
                     /translation="LGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTN
                     KANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEAT
                     KRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNS
                     GVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKY
                     LAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLML
                     SMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSK
                     EITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFN
                     RSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKE
                     FGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQL
                     AQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILRE
                     GFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNP
                     EIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          733 a          390 c          486 g          529 t
ORIGIN      
        1 attgggttat ttgcagatcg tgaacggcga agagatggag gcgcgagtga agtctacttc
       61 tacgattacg gtccaagaaa gtactcctcg cggaatgatc tacgactcca caggaaaagc
      121 tttagttacg aataaagcga atcaggccat cacatatacg agaggaacac aagttacggc
      181 taaagatttg cttgaaatcg caacgaagct gaactcgctg atcgatgttc cggtagatga
      241 aaatctgaca gatagagata aaaaagattt ttggctggct gatcccaaac atttgaaaga
      301 agccacaaaa agactttcgg caaaagaaaa gcaattaggg aacagcgaac aatatagtgc
      361 gatggtcaat aaagtcacag ctgatgagat taatttaaac gaggagcaat taaaaattgc
      421 taccatcttt aaacggatga atagtgcata tgaattaaac actgtctata tcaaaaactc
      481 tggtgttact gatcaagagt tagctgttgt ggcagaacgc gcttctgaac ttccaggagt
      541 ttcgactggc acagactgga cacgagaata taatgcagca agttcactga aaagtattct
      601 tggcagtgtg acgactgaaa agcaaggact tccagcagat gaagcagaga aatacctagc
      661 aaaaggttat tctcggaatg accgtgtagg acaaagttat ttggaaaaac aatatgaaga
      721 tgtcctgcaa ggaaccaaaa cccaatatga agtttccctt gataatgaag gaaatgtttc
      781 gaatcaaaaa gaaatcttct ctggggaaaa aggttctaat ttaatgttat ctatgaatgc
      841 agaattccaa tcaaaagtag aagaaatttt gaaaaggaat tatcaaacat tgatcaacaa
      901 cggcaaagca cagtattcgc ctggtgctta tgcagtggct atgaatcctc agacaggaga
      961 agtgctagca atgactggtt tcagtcatga acaaggttcc aaagaaatca ccgagaatgc
     1021 tttaggcacg attactagcg cctttgatcc agggtctgta gtcaaggctg gtactttgac
     1081 agcaggctgg gcaagtaacg cgatcagcgg aaatcaagtt ttgattgatg agccgattcg
     1141 tctgcaaggt gcttcagaaa aaagctctgt ctttaatcga agcggacaag ttgctcttga
     1201 tgcagtcaaa gcatcggaat tgtcttctaa tacgtatatg atcaaagtgg cgttaaagat
     1261 gttaggacta gactatacac ctggaatggg actacctagt ttagatgaag aagctaaagc
     1321 ttatcagcaa ttgagagact ctttcaaaga gtttggtctt ggtacaacga ctgggatcga
     1381 tttgccgaat gaatctcctg ggatctcacg ttctgttgat tatatgaaga agttcaatgc
     1441 ggataatggc aaagaatggt atgctccagg taactttacc gatttggcat ttggacagtt
     1501 tgacacatat acaccaatcc aattagctca atatgcttca acagtcgcta atggcgggaa
     1561 acgagtacag ccgcacctag tcaaagcgat ctatggcaat gacgaaaatg gaaacttggg
     1621 tgaagtgaag aaagaaattg gcacaacagt agaaaatacg gtcaatattt ctgcagaaaa
     1681 tatggcgatc ctccgtgaag gattccacca agttgtccat ggtaccgatc cttacacaac
     1741 agccaaacca ttagccagtg cgaaaatgga cctttccgca aaaacaggga ctgcagaaac
     1801 ggttgcggaa ggtcatccag atattgcgac agtcaatagt aatatcgtgg catacggacc
     1861 aacagacaat ccggaaattg ccatcagtgt tgtcttgccg aacttattag acgaacaaga
     1921 ccacataaac ttgacgattg ctaaagaaat aatggacgcg tattatgata tgttcatggc
     1981 aaaataaaaa agaggtcgtg aaaaagccgc ttagtttcga gtatgaagaa aaattttgtg
     2041 aaaatagctt ctcacatttt tgcttaaatt tgacttcata ccgagaaact ggcttttgaa
     2101 caccgtttac tcggcaaaaa aggttgtgac aaaaattg
//
LOCUS       DASJOX010000215         2132 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJOX010000215 DASJOX010000000
VERSION     DASJOX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.221"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            157..675
                     /locus_tag="U4A12_002796"
     CDS             157..675
                     /locus_tag="U4A12_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775115.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            683..1723
                     /locus_tag="U4A12_002797"
     CDS             683..1723
                     /locus_tag="U4A12_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1775116.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1972..>2132
                     /locus_tag="U4A12_002798"
     CDS             1972..>2132
                     /locus_tag="U4A12_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1775117.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          750 a          258 c          431 g          693 t
ORIGIN      
        1 tctaagtccg tatttaaata tcatgcttaa caatctggaa aaacttcagt tgcaaaaaat
       61 aagtatgtta ttgtttatag gtgtatgctg ttatcgtatg ttatttaaaa catctttgtt
      121 tggggattgt gctgttttaa ttgcactata tgtcatatgt tatacactga aaaaaacgat
      181 aataatattt ttgagagaca cagaatcata ggaacaattg tatgtataac agttatacca
      241 cttctcatat ccataggaat acttttcaaa tttataccaa atgcaatggt taaccagatg
      301 gttcatattg gttctccatt gatggttata gcaagtatat ttttatttta tgtatttaaa
      361 aatttttcat tttacagtaa gtatataaat aaacttgcga gaaccagttt aggggtatat
      421 attttacatg aaaatcatta tttcattgat ggtatgaagt ctagaggctt aatatgggat
      481 gggatatttg gattaggcaa aaggttttta gatagggatt tttggatttg gtttataatt
      541 gcagttgtag ttgtgtgcat aatttgcatg gctatcgatg ttgtaagaga aaaatttgaa
      601 agacagattt ttaaaattaa gagtgtaaag aacctgtgta aacttatgaa tcatgttata
      661 aatatatacc cataattaat aaatgaggag aaaagaagtg aaagttatat cattcggaga
      721 attattattg agacttgcag cacccggtta ttcaaagtta tttcaaagag atagtttgga
      781 gactacattc tgcggagggg aagctaatgt agcggtatcc ttagcaatat ttggatatca
      841 atcagaattt ataacaaagc ttccggattc agatgttgga tatgcggcag ctaatgcttt
      901 gcgttatttt ggagtaaatg ttgataatat tatatttgga gagggacgca tgggactgta
      961 ttatttagaa aaaggagcag ctcagagacc atctaaagta atttatgaca gaaaattttc
     1021 tgcaatttcg ttagctgata gtgatgaatt taagtggaag gagatcctaa acgatgcaaa
     1081 ttggtttcat tggacaggga taaatccagc attgtctgag aatctctttg agacgtcctt
     1141 agatgcatgt caggctgcca aaaaaagagg tataacaatt tcttgtgatt taaattatcg
     1201 atccaagctt tggacatcag aggaagcaaa agaaaagatg gaacagatac taccattggt
     1261 tgatatttgt atttgtaatg aagaagatgc agaaaaagtg ctaggaatta aagctgagca
     1321 tacaaatatt aatagtggag aattaaataa agatggctat gtgcaggtgg ctagggagat
     1381 tcacaagaag tttggatgta ggtatgtagc tacaactctg cgtagaagtt attcggcaag
     1441 tagaaatggt tggagtgcaa tgttatacga agcagaaaaa gataaagcgt atttttccac
     1501 agaatacaac atacaaattg ttgatcgcgt tggtggtgga gacagctttt ctgctggtat
     1561 aatatatgcg ttatgtgaaa atatgtccgg acaaaaggct attgaatttg cgactgcatc
     1621 tagttgttta aaacatacta tagaaggtga ttttaatcga actacaattg aggatgtata
     1681 taatttaatg aatagcggtg gaaatggtcg cgtgttgcgt tagtcaacat ataaagataa
     1741 atcacagtaa tttttatggt gattatctgt cttagtagtg attagaataa cgtgaaaata
     1801 aattgttgag cgtttatact gttttgaaaa ataaatttaa catattgaaa aatacaaata
     1861 tatacgtata ttataaagcg ccgattgtaa aattaagcta gacaactaaa acagtcattt
     1921 gtgctacact caaaatagtt ccgaccaaag aattataagg agtgagtgca aataacctat
     1981 acctatctta caacggatga acttgtaata atagagtctt atttgaaaat taaccaatct
     2041 gttgctaaaa ctgcgcattg cttgaatcgt tcaagtcaaa cgatccataa agtatatctg
     2101 ttcttcaagc aaagaaaatc agccttagaa ta
//
LOCUS       DASJOX010000216         2065 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJOX010000216 DASJOX010000000
VERSION     DASJOX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.222"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1008
                     /locus_tag="U4A12_002799"
     CDS             <1..1008
                     /locus_tag="U4A12_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775118.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="U4A12_002800"
     CDS             1025..1174
                     /locus_tag="U4A12_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775119.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="U4A12_002801"
     CDS             1171..1617
                     /locus_tag="U4A12_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775120.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="U4A12_002802"
     CDS             1619..1756
                     /locus_tag="U4A12_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1775121.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="U4A12_002803"
     CDS             1794..>2065
                     /locus_tag="U4A12_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775122.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DASJOX010000217         2056 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJOX010000217 DASJOX010000000
VERSION     DASJOX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.223"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            42..989
                     /locus_tag="U4A12_002804"
     CDS             42..989
                     /locus_tag="U4A12_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1775123.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1119..1859
                     /locus_tag="U4A12_002805"
     CDS             1119..1859
                     /locus_tag="U4A12_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775124.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          726 a          340 c          429 g          561 t
ORIGIN      
        1 attgatagta agaatgacaa catgttagaa aggaagagag catgcccact ttagcagatg
       61 ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat catccggaac
      121 aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg gattaccgtc
      181 caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa ttatttatac
      241 tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga atcgctaaac
      301 ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc gacgtcggtt
      361 cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt
      421 tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat gtagacagcg
      481 ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat tatcaaacga
      541 ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa actggttatt
      601 tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca aatcattcga
      661 cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa acttgttttg
      721 tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat gctggaggac
      781 acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac caaatcagtt
      841 cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta tgtggagaaa
      901 tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg
      961 aactagttat cagagagaca acgaattaag tcttattact atatatatat ttatgaaatt
     1021 catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat
     1081 tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac
     1141 atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc
     1201 gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac
     1261 agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg
     1321 caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat
     1381 taccggatta taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt
     1441 ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta
     1501 cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg
     1561 tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg
     1621 aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg acgccgtatc
     1681 aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg
     1741 aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta gaacgctcat
     1801 tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag
     1861 tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt
     1921 ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt aagcgaattt
     1981 tttctcccaa taagtaagaa aataatggca aaataaaaaa acaaactatt ttcgcagatc
     2041 gtttgcaaaa atagtt
//
LOCUS       DASJOX010000218         2003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJOX010000218 DASJOX010000000
VERSION     DASJOX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.224"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U4A12_002806"
     CDS             <1..378
                     /locus_tag="U4A12_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1775125.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U4A12_002807"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U4A12_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1775126.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U4A12_002808"
     CDS             1859..>2003
                     /locus_tag="U4A12_002808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775127.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASJOX010000219         1964 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJOX010000219 DASJOX010000000
VERSION     DASJOX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.225"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..414)
                     /locus_tag="U4A12_002809"
     CDS             complement(<1..414)
                     /locus_tag="U4A12_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1775128.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKD"
     gene            complement(411..1064)
                     /locus_tag="U4A12_002810"
     CDS             complement(411..1064)
                     /locus_tag="U4A12_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1775129.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1352..>1964
                     /locus_tag="U4A12_002811"
     CDS             1352..>1964
                     /locus_tag="U4A12_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1775130.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          617 a          350 c          252 g          745 t
ORIGIN      
        1 atctttcgca tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc
       61 atcattaagc tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt
      121 aacatttata ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac
      181 ttttatttca ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt
      241 tccattcata ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc
      301 caaatatgtt ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc
      361 aagaacagaa aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc
      421 tccaatttat atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta
      481 agttctttta tatatacatc tattactcga tcataaggag tttcttcgct atctttccat
      541 acataatcaa taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt
      601 aatacttcta gttcttttgc atttacatct atttcttcac catttatttt agctgtatag
      661 ctattaaaat taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc
      721 ttaattaaag aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat
      781 ccgtctacta aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag
      841 attagaattg gtaaattgct tttctttcta atttctttca acacttctaa accatttata
      901 aaaggtatct gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct
      961 tctcttccat ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt
     1021 ccctctctta tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa
     1081 gcctatacac tagatattat acagcatatt atggctctat aatatctagt gttggtgaca
     1141 ccttctgttg ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc
     1201 tttagaataa agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac
     1261 taacactttt tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac
     1321 caaaacatca ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg
     1381 attaacagat aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg
     1441 cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg
     1501 tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt
     1561 caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg
     1621 gcagaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact
     1681 gaagcaggcg attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa
     1741 gtacttcgtc tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca
     1801 gccacgtatg aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc
     1861 tgtttctggt tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat
     1921 ccttgaagat cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DASJOX010000220         1962 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJOX010000220 DASJOX010000000
VERSION     DASJOX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.226"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..272)
                     /locus_tag="U4A12_002812"
     CDS             complement(<1..272)
                     /locus_tag="U4A12_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775131.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="U4A12_002813"
     CDS             complement(309..446)
                     /locus_tag="U4A12_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1775132.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="U4A12_002814"
     CDS             complement(448..894)
                     /locus_tag="U4A12_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775133.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="U4A12_002815"
     CDS             complement(891..1040)
                     /locus_tag="U4A12_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775134.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..1386)
                     /locus_tag="U4A12_002816"
                     /pseudo
     CDS             complement(1054..1386)
                     /locus_tag="U4A12_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DASJOX010000221         1848 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJOX010000221 DASJOX010000000
VERSION     DASJOX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.227"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..249)
                     /locus_tag="U4A12_002817"
                     /pseudo
     CDS             complement(31..249)
                     /locus_tag="U4A12_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(515..709)
                     /locus_tag="U4A12_002818"
     CDS             complement(515..709)
                     /locus_tag="U4A12_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775135.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(809..895)
                     /locus_tag="U4A12_002819"
                     /pseudo
     CDS             complement(809..895)
                     /locus_tag="U4A12_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          645 a          264 c          245 g          694 t
ORIGIN      
        1 atgtcggtcg gttgattgat atagctaaag ttataactca accgaagccg attcatctac
       61 acgaataaag ttgcgagtgt tctcggctaa ttcataaaaa ttgttatgtg ttccatatag
      121 caatactgga ataactttat tttcttgaat ctcataagca actctataca cggtgaccgc
      181 atacttaaat ccattagacc aattacctga taaacctcca gttttttgct cttacttaaa
      241 tggatcaatg gcaatttcat cacaaaccta tagaggatga gtgacttctg cctaattacg
      301 ttaaaaaata ttgaaagtaa ctctatgtag caaaaaatat ctagaatttc atttcctcaa
      361 aactgatcga tattgtatct aaaaatatat actacctaaa ataggtagta tatattaaaa
      421 tatgtgcttt tgttagaaaa atcaatacat aaattatcat atctgtatat gtcatttagg
      481 tatttcagtt atgttataat agttttgctg tctgctaccc cggcctaatc tggcaagggg
      541 aggaggagat ataatgaaat acctttttga ttttgttttt gcaatcaagc cacttgcaga
      601 tgtagtgtgc agttacctct gcaaatggct tgattgcaaa attgccaaaa aagacagtaa
      661 gcctacccgt taattctata atatagaaac gtaaaaaaaa ccccatcagc ctgcacgctg
      721 atggggtttg gtgatataat gaaatacctc tttgttactc ataattgtac cataattagt
      781 cacgaatgca accaatagaa aaaactactt agaacggtag atcatcgcta ataacaatgc
      841 attgtccacc aaatggattt tgtgaagcat taaatacatt gttattaatt gtcgctgctt
      901 ttaactaggc ctctcccctt ctattataaa agaagatata tatcatttct taattaccta
      961 ctaccttaaa ccaaaattta agtactacct aatttaagta ttacttaaac aatctaaaaa
     1021 atagttatcc caattgttat gcttttataa tacgattatt tgtaacaaaa tgaatattat
     1081 ataattaaac actaccatat ttaggtagtg tttaattatt agcaaaacaa aaaatattta
     1141 ttctattctc ttaacttcaa ctaagttatg ctgcacacct ttatataaat catatgcaac
     1201 tcattagcta gacaaactac aagcatgtag ttttatctgt taattatgta ctacctaaat
     1261 aaagtagttt ataattagca agtatatata tttaaaaatt taagattaat ttttttatga
     1321 cttgtacaaa tttatctgat attctcttat taagaactac ctaatttggg tagttcttat
     1381 tttttacatt ttaagtagta tttaattgca atttggtagt atttttttat tatcttattc
     1441 ttagaacggt agtacttaat ataaatatag gtagtatcta atttaagtat taatggtagt
     1501 atctaaatta acaggtagta tgtaactact ttaaagtaat ttttttcaga ttaacacagt
     1561 agtatcttat taaataagtt aattttaatg gtagtacata attattatat atatctgaca
     1621 ttggtagtac ttattgtatt ttttagtagt atataatcta ttgttttcta gcctaaagcc
     1681 ttgtcttata agaataatat aatatatact actgaaataa ggtagtatat attactagta
     1741 atggtagtat gttttatatg cataaggtag ttcttaattt attttttgtt ttgacactgg
     1801 tagtacttaa ttatctttaa ttttaaaact aaattttatt catacgtt
//
LOCUS       DASJOX010000222         1847 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJOX010000222 DASJOX010000000
VERSION     DASJOX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.228"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            145..711
                     /locus_tag="U4A12_002820"
     CDS             145..711
                     /locus_tag="U4A12_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1775136.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            856..943
                     /locus_tag="U4A12_002821"
     tRNA            856..943
                     /locus_tag="U4A12_002821"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:888..890,aa:Ser,seq:cga)
     gene            1277..>1847
                     /locus_tag="U4A12_002822"
     CDS             1277..>1847
                     /locus_tag="U4A12_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN1775137.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          646 a          277 c          356 g          568 t
ORIGIN      
        1 tttttttaat tttgcaaaaa aaagttaaag aagactgaaa aaaaatttaa aatataacgt
       61 tttgagtaaa atgtttgcaa aaaatgctaa aaatagggta caataaaaca acatatactg
      121 cagtaaagaa gtgggggagt tttaatgaga agaaaagtct ataccaagga tcaaattcta
      181 aaagctgctt atgatgttgt tgcaaaggaa gggtttaaag gatttactgc gagaaatatt
      241 gctaaaaaga tggggatttc gacacagccg atttatctgg aattcaaaaa catggaagat
      301 ttaaaaaata cgttgctgga ttctatcttt gaacaactaa aagaagatgt tttccctatt
      361 gaacgtactg gtgataaaat cattgatctc ggtttgaact atatctactt tgcaaaaaag
      421 catcatagtc tttacatggc attgtacatt gatgaacatg gggacggaaa gaaaatgcat
      481 gatttttcat acaattattt ccgtgaaata gttaaagaag atgaaagata caatcaatta
      541 tcggatgaat atgtcgatgc tctgcatact ggtacatggg tagcagtgac tggcttagcg
      601 tcattgatgt catcaaacat catgcatcca acagaagaac aaatcattgc actgatcaaa
      661 cggacaattg actcgatttt agcattagat aatcctgaaa taatcagata gtgttctatc
      721 agtatgaatt tgatcggtaa gtccgttggc tgcaaactgt caatggactt ttttgctaaa
      781 aaaagcgatt tctgttattt atctattaga aataggcttg acatttcgtg aaaaaagttt
      841 aaaaatagta cttacggaag gttggcagag tggtaatgca ccggactcga aatccggcga
      901 accggctaat accggcgcgc aggttcaaat cctgtacctt ccttatatca acgattagag
      961 gttgtgaaga agctgtcctg catcaagtaa taagaacggc caaacaaaaa tagcttttcc
     1021 tattttttcg tttgattggg cttattaccg cagatgcaag cttttgaaca ccgtttgttc
     1081 ggaataaggg gctgtgacaa gacttttgtc acagcccctt attgttgtac ttttaaccta
     1141 caatattttt tgattccccc tgagtactat tttttataaa tgctagtaaa tcaatcttct
     1201 ttggcctttt tttgataaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     1261 tttaggaggg gaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     1321 aaggaatttt gattatgaga tgtcttataa tgaagtggtc actattactc caagaaatta
     1381 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     1441 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     1501 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     1561 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     1621 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     1681 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     1741 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     1801 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaa
//
LOCUS       DASJOX010000223         1845 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJOX010000223 DASJOX010000000
VERSION     DASJOX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.229"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..375
                     /locus_tag="U4A12_002823"
     CDS             10..375
                     /locus_tag="U4A12_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1775138.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..1815
                     /locus_tag="U4A12_002824"
     CDS             376..1815
                     /locus_tag="U4A12_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEN1775139.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          772 a          137 c          313 g          623 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
     1141 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
     1201 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
     1261 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
     1321 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
     1381 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
     1441 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
     1501 agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga
     1561 gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa
     1621 gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag
     1681 aaagcaaaag aatatcaaga tatagttgaa gaatattatc ctattgaaac tattgtttat
     1741 ggaattaaaa atattaaaca ggaatttatc gaaaatggta gaaaagaaat ttataaaagg
     1801 acttataaag attgattata taatatatga aaagctatta taaaa
//
LOCUS       DASJOX010000224         1800 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJOX010000224 DASJOX010000000
VERSION     DASJOX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.230"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            30..1382
                     /gene="brnQ"
                     /locus_tag="U4A12_002825"
     CDS             30..1382
                     /gene="brnQ"
                     /locus_tag="U4A12_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1775140.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          310 c          353 g          631 t
ORIGIN      
        1 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
       61 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
      121 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
      181 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
      241 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
      301 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
      361 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
      421 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
      481 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
      541 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
      601 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
      661 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
      721 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
      781 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
      841 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
      901 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
      961 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
     1021 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
     1081 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
     1141 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
     1201 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
     1261 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
     1321 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
     1381 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
     1441 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
     1501 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
     1561 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
     1621 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
     1681 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
     1741 ttcttttata atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DASJOX010000225         1791 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJOX010000225 DASJOX010000000
VERSION     DASJOX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.231"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            60..586
                     /locus_tag="U4A12_002826"
                     /pseudo
     CDS             60..586
                     /locus_tag="U4A12_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            741..1316
                     /locus_tag="U4A12_002827"
     CDS             741..1316
                     /locus_tag="U4A12_002827"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1775141.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1359..1526)
                     /locus_tag="U4A12_002828"
     CDS             complement(1359..1526)
                     /locus_tag="U4A12_002828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775142.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            1543..1725
                     /locus_tag="U4A12_002829"
                     /pseudo
     CDS             1543..1725
                     /locus_tag="U4A12_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          629 a          288 c          353 g          521 t
ORIGIN      
        1 gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata
       61 tgaacgattt tactacagaa atcctaaaga ctctagcgaa caaaggcgat ttgaatggat
      121 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
      181 tcctcgatta cgaaaagtac gatcgcattg gttttaacac gggtaattct cgtaacggct
      241 cctatgaccg tacggtcaag accgagtatg gggaacttca tctccagatt ccgcgcgacc
      301 gcaatggcga gttcaagcaa cagactgttc ctgcttatag acggacgaat gacacattag
      361 aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga
      421 ttgagaaaat gtatgggcat cactacacgc cccaaaccat gtccaacatg actaaagtgc
      481 tgactgaaga agtaaatgca tttaaagtta gaactctaaa tgataagtat gtcgctattt
      541 tcatgattcc ttcagttttt cagatgatat accactcaaa aaacaatatg agagatgttc
      601 aggaatacca tctctaatgg cttataaatt ggttttaggg atagtagatg ttatagacat
      661 atcaaggaat aaacgagcag ctgcataaaa gcagatggaa ttaaggaata attgaattgt
      721 aaaagaaggg aataaattta atggcacaat tattttacag atatggaaca atgaatagtg
      781 gaaagagtat tgagctactg aaagtggctc ataattatga agaacaaggg aagagagtta
      841 tcataatgac aagcgcaata gatactcgat caggatttgg aaaagttgag agtcgtatag
      901 gtgtttcgaa accagctatt gcaattactt ctgaaatgga tgtgtacgga gaattagtgg
      961 agattattga tcagtacgga aaagttgatt gtattttggt cgatgagtct caatttttaa
     1021 gtagagagaa tattattgat ctatcactta tcgtaatgaa tgaggaagta ccagtaattt
     1081 gctatggatt gaagaatgat tttcaaaata atctcttcaa gggatccgaa gcattattga
     1141 catttgctga taaaattgag gaaattaaaa ctatttgcca tttttgcaat aaaaaggctg
     1201 taatgaatgc tcgtattgtt gatggtattc cagtttattc aggtgaacag attgtagtag
     1261 gaggtaatga agattacctt tcggtatgtc gcaaccacta ctacaacgaa gattgaaata
     1321 aaaaacgtgt aatcaaataa ttgattacac gttttttatt attctgttaa aggttgagga
     1381 tcagaaattt ttaggtatct ataatcctca cctaaaaatc cgttaaaacg aagtaaaaca
     1441 tcatgaccat ttaaatcagt tggaactaaa attggaatta atttatcaac agtagttttg
     1501 ttgtaaagtt caaaagtgtt ctccattcct tctggttcta caatcgaatc tttcaataaa
     1561 caaatcaaga aatacagccg cagaaaagag cagtttcaaa atgaagaatc actagagcgt
     1621 ttcctagtat ccatctttga tacatacaat caaaaattct taaatagaag ccataaaggc
     1681 ttccaacaag tgacggatac attagcttca atgtttactg agtaacccat tattttgcag
     1741 aaggacgagt tatttacaca aaattattga cgctccctgt ttggagtggt a
//
LOCUS       DASJOX010000226         1789 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJOX010000226 DASJOX010000000
VERSION     DASJOX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.232"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..161)
                     /locus_tag="U4A12_002830"
     CDS             complement(<1..161)
                     /locus_tag="U4A12_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1775143.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQE"
     gene            370..582
                     /locus_tag="U4A12_002831"
     CDS             370..582
                     /locus_tag="U4A12_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775144.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            744..1262
                     /locus_tag="U4A12_002832"
     CDS             744..1262
                     /locus_tag="U4A12_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN1775145.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1269..1781
                     /locus_tag="U4A12_002833"
     CDS             1269..1781
                     /locus_tag="U4A12_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775146.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          685 a          233 c          273 g          598 t
ORIGIN      
        1 tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg atcatataag
       61 atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac ggctacaata
      121 atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc tcctgctatc
      181 tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca gaaccctaaa
      241 aacactatat attccgaaaa gtgagtatct ccattcataa aataatcaac atatttaaaa
      301 aatctaatta gcttttttag tcaatctaaa gttctttatt ctatgatata atgttactgt
      361 agaaagtttg tggcggtttg gcaaatccaa ttgaaggagc gtggtgccta tgggcttatt
      421 agcttatcgg acgtactctg tagaaaggaa atgcctgatt tgttctcatt ggatcaaatc
      481 attgctatta tcggagcagc agcagtagtt attactgccg tagcgaaagc tagcgattcg
      541 attttagctt ggttaaagtt caaaaaagaa caaaaaaaat agagccgtct caacttttgg
      601 cgaggtagac gagctctaaa gctttattta catgccatcc gctttgaacg gactacactg
      661 ggcatgtgac agcatgccct ttcatttata ttatatctca ttcttttata taatgaaagt
      721 aaataaatca tggaggataa aatatggata aatttaatag attaacatta ataaatcagt
      781 ttgaaatttt aaaagcttta aatcctaacg aagaaaaata ctatgctgaa aaaatagaaa
      841 tactaacaga aggttacgaa tatcactata gtgaaatatt tgaaaatatt tctgatccat
      901 tgccagaaga agattcgcgg tttgttctag acatattaaa tatgtataga gatattactt
      961 ttagtaaaaa taaattaaaa gatataaatt caattgataa ttattatata caatttaaag
     1021 gttttgactt taatagctct accgaattta agttagcact ttatgcacaa ttttttattg
     1081 aaaaattaaa taggtttcaa gaaatagttg aagatagtga ttttaacact tttaatagcc
     1141 ataaaccgat gagaggtact tatataaaat ttctcgaaaa ttataatgat ttaaaatcta
     1201 ccaataatta tcaatttgga aacttgtctt atgagatgat aagtaaagtt ctaagtttgt
     1261 gatgataaat gaaattaaat agtataaatg atttatttgc tattataggt gttattgcta
     1321 acatactttt ttcgtgtttg aattttagat ttactcaaaa gaacaaaaag aacaaactca
     1381 gtatagaaca aatgaaaaaa gacattgaaa ctgatttaaa aaatgaccat aaagaaagaa
     1441 gaaaaattga agaatatact tctttaatgg aaaagtgtaa agcaactgat gcaattttta
     1501 gctttttaag aaaaagtgat gagataatca cacgtgaaaa tatagattta gaaaatgaga
     1561 ttagtaaact tgaatctatc ttttccgata taaattctct taagtattta atcgtcgaag
     1621 aaaattcgga tagcttttta gaatttgaaa aatatctaac agatcttata gataatagaa
     1681 ataatgaatt aatgagacaa aacattgcaa gattccttac taaattagtt tctgaatgga
     1741 aaagtattat ttctcaaaat atggataaat taagatatta aggatgtta
//
LOCUS       DASJOX010000227         1760 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJOX010000227 DASJOX010000000
VERSION     DASJOX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.233"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..351
                     /locus_tag="U4A12_002834"
     CDS             <1..351
                     /locus_tag="U4A12_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1775147.1"
                     /translation="NEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYL
                     PIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDI
                     EIDILDEYKHDILI"
     gene            407..625
                     /locus_tag="U4A12_002835"
     CDS             407..625
                     /locus_tag="U4A12_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775148.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            696..1511
                     /locus_tag="U4A12_002836"
     CDS             696..1511
                     /locus_tag="U4A12_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775149.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            1575..>1760
                     /locus_tag="U4A12_002837"
     CDS             1575..>1760
                     /locus_tag="U4A12_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1775150.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVF"
BASE COUNT          683 a          203 c          301 g          573 t
ORIGIN      
        1 aatgaagatt ataccaagat agattgggga gatccaattg atcatatcat ccgacacaga
       61 actttattga aagatttttc gaattttgtt tctattaggt cagacggagg attaatggca
      121 tcaccttact tgccaattca agttggaaat attaaatgtc acagtctttc taaatattgg
      181 cgagcaggac tttataaagt tttttcttta gatttagttc aagatatagc tactcaagtt
      241 tcttcaatac aagatatgag taaagaatta aaaaaaggac atattaaatg gcttaatgaa
      301 gatatagaaa ttgatattct cgatgaatac aaacatgata ttttaattta ggtttatcat
      361 tggaaagttt agataaacaa agtactttta gaaaggaggg aaaaaaatga tttctgaacc
      421 tcaaatgaat aatatgtatg aaagcccaac tataatggga gctggtgaag ctgcgccaag
      481 agcttgttca gcaattatgg tgtgtgtgtt attagcatct gcaggagtag gtatccaagc
      541 aggagcggtg tatacttatg ctgtagttgc tacagtagct gtagctgcag ccgcagtttg
      601 gaaaacagta ggattagttg gttaggaatt aataggagac taactgtctc ctattgattc
      661 caatgattaa gctaaggagg aattttaaga aaataatgaa atacaataat ttaaaaccga
      721 aattacttaa tgagaacata gaatatataa gagaagaaaa ttatggatat ttaaccatag
      781 ttcccaaaaa tggaactgaa ttgaaagata acgttttcaa tccaacagta agatatatat
      841 tggctaactg tactggaaaa aacagtgtaa aaaaaatagc gaatggttta aaaaagaagt
      901 ttcctagttt agaatatgaa aaattctatc aagatgttgt aggaactatt aaatttttca
      961 aacactataa tattattgat ataggagatt atagtatgga agaaaagaaa atatataaaa
     1021 ttgaacaaaa tttagaatta gaattactaa atgaatcaca ttatgaagaa atttataatt
     1081 ttatcacaga tcatcctgaa agtatgtatt ttcatgaaga tatttatcat caaggaaata
     1141 gtaaagaatt agaagttgtc cttagatata atttattttc gtttacgagt gaattttact
     1201 ttttaagaca taaagagaga attgtaggaa tggtttctat aattgttaat ccatttcaaa
     1261 acttatccta cagtatagta aattacctca tatgtgagcc tgcatatact acgactattt
     1321 tgacaacagt attagaaaat attaacgaag tttcaatttt agaagtaaag aagctagtga
     1381 ttcatttgct aaattcaaca gaagatgaat cactaatccc ctatttaaaa gattcacatt
     1441 tttttaaaga agcggtacta tctaaagaat atagagagca tgatgttttg agatacgctt
     1501 acatttatta aataaaattt ctgatagtat tttaataatt cggatcatat cataagatat
     1561 aggagggaaa agaagtgatt ttattgattt tgtctctcgt attactagtt gtatctaatc
     1621 taatgaaaaa taatcctcct acaaaaatta attcttttta tggctttaga actaaaaagt
     1681 caatgaaaaa tgaaaaaagt tggcatttag cacaaaaatt gttgggggat ttttcaaaaa
     1741 aaaaagttat cccagtattt
//
LOCUS       DASJOX010000228         1674 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJOX010000228 DASJOX010000000
VERSION     DASJOX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.234"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1438
                     /locus_tag="U4A12_002838"
     CDS             <1..1438
                     /locus_tag="U4A12_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1775151.1"
                     /translation="NRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDV
                     TPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIG
                     GTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPV
                     EVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGN
                     KKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKE
                     DLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPI
                     TEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDES
                     GNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDS
                     NIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          660 a          256 c          359 g          399 t
ORIGIN      
        1 caatcgttcc gatgccgata aatatgaacc aacagtagaa ggcgaaaaag tagaagttgg
       61 gggcacagta gatctaacag ataatgttac taacttgcca gcgttaccag aaggaacaac
      121 agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggta attacgaagg
      181 tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt
      241 gacagacaat cgttctgacg ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga
      301 aattggaggc acagtagatt taacagataa tgttactaac ttgccagcgt taccagaagg
      361 aacaacagta acagatgtta ctcccggcgg tacaattgat accaacacac caggcaatta
      421 tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa gatacggtga aagttccagt
      481 agaagtgaca gacaatcgtt ctaatgcaga taaatacaat cctgaaggac aaaaagtgac
      541 tacagactta aataaagagc cagatgcatc tgagggaatt aagaacaaag aagatttacc
      601 aaaaggaact acttatgctt ggaaagagaa agtagatgtt agcacagctg gaaataaaaa
      661 aggtactatt gtagtgacat atccagatgg atcatctgat gaagttgaag tagacgttac
      721 agtgacagat actcgttccg atgcagataa atataatcct gaaggacaaa aagtgactac
      781 agacttaaat aaagagccag atgcatctga gggaattaag aacaaagaag atctaccaaa
      841 aggaactact tatacttgga aagaaaaagt agatgttagt acagctggaa acaaaaaagg
      901 tactgtagta gtaacatatc cagatggatc taaagaagaa gtagaagtta ctatttctgt
      961 agtagataaa aaagcaccga ataaacctca agttgatcct attacagagg gtgaccaaat
     1021 tgttactggt aaaactgaac caaatgcaga ggtgacagtt acattaccag acggaagtca
     1081 acaccatggg acagctgata aaaatggtaa ttttacagtt aaagttccta aattagaggc
     1141 agggacaaaa gtgatagtaa ctgcaactga tgaatctggt aatactagtg aacctactaa
     1201 tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac ggtagtaaag gtagtaagac
     1261 tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga ggcaaaagcc agtcctcaaa
     1321 agtttttcct aagacgggag agagtgacag taatatcttt actattagcg gtggtttaat
     1381 actgttagga acgttaggat tattagggta taaaaatcga aaaaaagaga atgaatagaa
     1441 aatcaatttc ttaagtcttc tctgagaaga ggaagaatct ttttcttcct ctttttgctt
     1501 ttttatgatg ggaaataagt gttagttaaa gatgaatata ctttttatag aaaggaaaag
     1561 aatgtaatta agtggaaaaa atagtaatta ttggttcaaa ccattcaggg ctagccgcag
     1621 ctcgatttct aaacttaagc gattcaaatt atgaagttat tctaatcgat aaaa
//
LOCUS       DASJOX010000229         1672 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJOX010000229 DASJOX010000000
VERSION     DASJOX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.235"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..1622
                     /locus_tag="U4A12_002839"
     CDS             75..1622
                     /locus_tag="U4A12_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN1775152.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          560 a          305 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DASJOX010000230         1642 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJOX010000230 DASJOX010000000
VERSION     DASJOX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.236"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..>1642)
                     /locus_tag="U4A12_002840"
     rRNA            complement(1..>1642)
                     /locus_tag="U4A12_002840"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          348 a          507 c          350 g          437 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
      961 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcaa ttcgtacctt
     1021 cgcttgcgct aagcactcct cttaaccttc cagcaccggg caggcgtcag cccctatact
     1081 tcatcttacg attttgcaga gacctgtgtt tttgataaac agtcgcttgg gcctattcac
     1141 tgcggctgat cgtatgatca gcaccccttc tcccgaagtt acggggtcat tttgccgagt
     1201 tccttaacga gagttctctc gctcacctta ggattctctc ctcgactacc tgtgtcggtt
     1261 tgcggtacgg gccgctgttt tctcactaga agcttttctc ggcagtgtga catcaggaac
     1321 ttcggtacta ttatttccct ccccatcaca gcttgtcctt aagaaaagaa gcatttgact
     1381 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
     1441 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
     1501 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
     1561 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
     1621 ttctcacttc taagcgctcc ag
//
LOCUS       DASJOX010000231         1634 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJOX010000231 DASJOX010000000
VERSION     DASJOX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.237"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..868
                     /locus_tag="U4A12_002841"
     CDS             101..868
                     /locus_tag="U4A12_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1775153.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="U4A12_002842"
     CDS             1060..1458
                     /locus_tag="U4A12_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1775154.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DASJOX010000232         1603 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJOX010000232 DASJOX010000000
VERSION     DASJOX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.239"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..819)
                     /locus_tag="U4A12_002843"
     CDS             complement(<1..819)
                     /locus_tag="U4A12_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1775155.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSM"
     gene            complement(852..>1603)
                     /locus_tag="U4A12_002844"
     CDS             complement(852..>1603)
                     /locus_tag="U4A12_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1775156.1"
                     /translation="AHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGES
                     LFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLL
                     EIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDV
                     FVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIK
                     EPYANKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT          523 a          266 c          188 g          626 t
ORIGIN      
        1 catggaagga tcaataatat gaatatttgc taatttcgaa gtttgtaaag tagtttcttc
       61 aatatttttc aattcaagtg gtaaatctgg tcctccaata acatttttat ttttgaacga
      121 ggaattaaaa aaaagtgtat gaaaaaattt ttcatctggg gcaaacatac ctattttaaa
      181 attcaggtag tcttgttgat tttgttcata catttgtatt aattctgtta ggcattcttt
      241 cgacaaagcc caccattgag aaccatgata caaatcaaat ttatggtgtc taatttttgg
      301 gggaagttta atcatcctca gacaacgatt aattatagct ctaaaaattt taaatgcagt
      361 tgtgttattc ctattaattc taggataatc atgtttttga tatacatcta tatgtttata
      421 ataaaattct ttatgtttaa tttgatctag atctattcca cgaataaact ccactgaact
      481 attcttttta aaataattat aaatatactc attatctttg attggataat cagcaccaga
      541 taataataca tattttaaat atttctcatt ttgtaatgct ctctttacta aattgatttc
      601 tgcttgtaca atggaaaaac caccccaata aattttaaca cggtttttat tacataaaaa
      661 ttcaacatta tcatatgatt taaatgcttc aataaatggt tctatatcag tttttttatc
      721 tatatgaata ataaaaactg cattttcgtt caatcgattg accaatctct ttaagttgcc
      781 aatatcattg tatgccaata tcaaataaca tattttcaaa aataatcact cctaaaatat
      841 cttgaacatt tttaatgatc tctactatat atactctcca tttgttcagt agtgtacgaa
      901 acatcataac tctttagttc ttgtgttatt ttattagcat acggctcttt tattactttt
      961 tttgcccatt cttgtggtgt ttcatctaat gatacaaaat ccacattatt tgttaacttt
     1021 gtatctttgg aaatagtatc tgctaagact aattttaatt ttgctgcttg tgcttctaat
     1081 ccaaccattg gcagaccttc ataaagagaa ggcattacaa aaacatccat cacagataat
     1141 aactcgggaa tatcgtttct ttctcctaaa aattttactc ttttctctat atccaattct
     1201 actgctttgg ctttcagttt atttctaagt gacccttcac ctactaaaat caagtaagaa
     1261 tcagaattca tttttagtat ttctaaaaaa atctctaaca aataagattg atttttttgt
     1321 ttctctaatc gtccaatgtt tcccacaaca aaagtactca ttgagatatc caattctttt
     1381 ctcttattaa ccttagcttc tgaattttta ctataaagct ctaaatcaat accattatga
     1441 actatctcaa agggatggtt ttgaaacaga ctttcaccag cttcttgact aacggcaagg
     1501 tagtctgttg cattttgtaa caacaatttg gttaaaaatt tacgcaatac attcattaca
     1561 atattttgag attttccatc ttcagttgca tggctatgag caa
//
LOCUS       DASJOX010000233         1562 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJOX010000233 DASJOX010000000
VERSION     DASJOX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.240"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4A12_002845"
     CDS             131..505
                     /locus_tag="U4A12_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1775157.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4A12_002846"
     CDS             complement(619..1248)
                     /locus_tag="U4A12_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1775158.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U4A12_002847"
     tRNA            complement(1382..1454)
                     /locus_tag="U4A12_002847"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          536 a          232 c          271 g          523 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 ta
//
LOCUS       DASJOX010000234         1539 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJOX010000234 DASJOX010000000
VERSION     DASJOX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.241"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..433
                     /locus_tag="U4A12_002848"
     CDS             179..433
                     /locus_tag="U4A12_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1775159.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            434..751
                     /locus_tag="U4A12_002849"
     CDS             434..751
                     /locus_tag="U4A12_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1775160.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1385..>1539)
                     /locus_tag="U4A12_002850"
     CDS             complement(1385..>1539)
                     /locus_tag="U4A12_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1775161.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT          546 a          230 c          289 g          474 t
ORIGIN      
        1 agtgtatggt gagtagttca ctcgtatttt gatatcgttt gtatatgttt tcagcgttct
       61 aagtggctta ttttgtccaa taataatatg gagtggaatc ccttaagaaa caccgttggc
      121 aattgttgtt ataacatgtt ataatttatt catcaaaaac taaaaggagt gtgataacat
      181 gggaaaagaa ctaaaggtta gaaagatcgg caatagcgtg ggagtgatcc ttccaagctc
      241 tcttggatta aaaagcggag acattatcca agccaaacaa gaagacaact tattcatatt
      301 agatactact caaatagcaa aagaacacga tcgcaaattg attgaagaaa gtttccaaga
      361 ctttgagaaa ggtttaacag tttcagaaat tgaaatggta aaagctttcg gaaaatacgg
      421 ctggagtgaa tagatgaaag agtacacgct agtttattct aaaagttttc agaccagcct
      481 tgaaacgata atagaagaat gggaaaatga gcttgctctt tctccagcca gcataaaaag
      541 atttgtaggt gcgatccaga aggctttaga actaaccaaa aattttccaa ctatgcatga
      601 ggaagtttcc gaaatctatg gaatggatat cccaacatat cggatcgtga tcggcaaaag
      661 ctatgcctta ttttatcgga tcgaccatga aaaaaaagag atacttgtag gacgtatttt
      721 ccaatctaaa caaatgaaac taggctttta acaataatta ggaaaaagtc gctcatcctc
      781 tacaggtggg tgattaatgc cgctcgatat ggttgttaac cgtcggttac tcgaagattg
      841 atatataaga cttagtacct ttttattttg ctccatgtta tggccgaagg tctaaagata
      901 caatcattag tctagagaaa aattaagaac cttaggaact acggggatcg cttggtgaat
      961 aagagacact gctattagtg aaaaaataag atatcaagta cgctctattc ccaaaaattt
     1021 cgtgacttta ctcatgagag gttcaagtag tatattttta atcaaaattg acgtaacttt
     1081 taataaatac tacctaaata aggtagtatt tatttttgtg ttttaaaaga ggaagtccaa
     1141 tatatctatg ggaaacttct taaatattat gttggtacat gaaaatatac ttccctgcta
     1201 gactatttac aacattttac tttgtacgta ttcttgtttt aaaaggaatg ggataaaata
     1261 taggtacaat aactatttga tgctaaatat aattaatgga ggagagatag tgaacatact
     1321 taatgaaaaa agatatgtat ggtttgctgg taaaaaatat attaacccgt ggtgctagtt
     1381 aattttaata tcctaaagaa tatctcaaag ttccttcaaa acgtcgttgt gctcgactta
     1441 atagaacact gtaagccagt aatatgcttt caaatctact ctctagccct ttaacagaac
     1501 gtgagttgaa attttgacaa cctaattttt ctaaggaag
//
LOCUS       DASJOX010000235         1527 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJOX010000235 DASJOX010000000
VERSION     DASJOX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.242"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..1483
                     /locus_tag="U4A12_002851"
     CDS             17..1483
                     /locus_tag="U4A12_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN1775162.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          419 a          289 c          324 g          495 t
ORIGIN      
        1 aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg
       61 attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc
      121 attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc
      181 aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg
      241 cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt
      301 cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc
      361 gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg
      421 acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg
      481 tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa
      541 ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat
      601 catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac
      661 tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt
      721 attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag
      781 tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt
      841 aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat
      901 tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac
      961 acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat
     1021 tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca
     1081 accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct
     1141 attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt
     1201 gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt
     1261 gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc
     1321 tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga
     1381 atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac
     1441 tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac
     1501 aggaattgct tatttgcagc agttcct
//
LOCUS       DASJOX010000236         1467 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJOX010000236 DASJOX010000000
VERSION     DASJOX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.244"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            286..693
                     /locus_tag="U4A12_002852"
     CDS             286..693
                     /locus_tag="U4A12_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775163.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="U4A12_002853"
     CDS             707..1108
                     /locus_tag="U4A12_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775164.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..>1467
                     /locus_tag="U4A12_002854"
     CDS             1110..>1467
                     /locus_tag="U4A12_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775165.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT          562 a          178 c          258 g          469 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataa
//
LOCUS       DASJOX010000237         1454 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJOX010000237 DASJOX010000000
VERSION     DASJOX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.245"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..1246
                     /locus_tag="U4A12_002855"
     CDS             158..1246
                     /locus_tag="U4A12_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775166.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1243..>1454
                     /locus_tag="U4A12_002856"
     CDS             1243..>1454
                     /locus_tag="U4A12_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1775167.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFL"
BASE COUNT          562 a          166 c          242 g          484 t
ORIGIN      
        1 acactcaaat gatttttctc ttagtaccta tgatagtttc gtgggttgat aatcaacaac
       61 gaatatcgga atagatagct aaagtatatt ttaattatga atttataatt agttgtaaaa
      121 gttaatattt aacggacagg aagtagggtg aaaaaatttg aaaaaaattt tagtgttagc
      181 aaatacatat tatcaaataa tagtagcaat acaaatgaaa aatacaattt tttccgatga
      241 gtatgttgaa attattattt ctgatcattc taaaaacact aaagaaattg cgaaaaaaat
      301 agaagaaaaa aaagtgtttg atggctgtca ttatttagaa agcaaatctt ttgatgataa
      361 tattgggtat tttcaaaaga ttaaggactg tcttcagatt tcttgtggta aatcaaatcg
      421 atatgcctat tatttgaaaa actgtaaaag tgaatatttt gatgaattta tttgtttttc
      481 aatgggagag gtttggttgg aaggaatata tgcttgctta tatcgaaata ataaaaatgt
      541 acaggtatca attttagaag aaggtatttt aagctatgga gttgacatta ttccaacagc
      601 aagaacaaaa gctatcgcag tactcaatta cttaaaaaaa aggactccac tatataacaa
      661 aagctataat ttttattgtt tttttcctaa tatatataca ggaagagaga atacaataca
      721 aattccatta gttacagctg ggtcaagaac cgcattgcaa ctttcggata tttttaaccc
      781 tatgatagat atttataaag aaaaaaaata tatatttttt acgtctgttt atgattttga
      841 gggcggagaa cctgtcggag aatatgaatt agtatgcaaa atagcagact tagtaggaaa
      901 agataatcta attgttaaga cgcatcctag agatgtaaga acaatttata aagataatgg
      961 atttatcgag gataaaaatt catcaatacc ttgggaggcg attcaattgt caggcgattt
     1021 taacgataaa atatttatga caattaattc aggaagtgtt atttcaggta gtacaatgtc
     1081 tgcaaaacct gtacgcacgt attatatgta taaattgtgt aacatagaag gaaatccgtc
     1141 ttgcaaaaag aatgcgtatg atattgagcg attgcttaag caaaaaacga tgaaaaacat
     1201 tttaaagaat gtgaaaatag cagaaagctt agatgatata ctatgagcaa aactaaatat
     1261 ttaattaaaa atatgggaat attaacaata agtaattttt cttcaaaaat tttagttttt
     1321 ttgttagtcc ctttatatac aagtgtatta tccacaacag aatatggcac atatgatttg
     1381 acaatttcaa cagccacatt attgtttcca attttaacct taaacattgt agatgctgtg
     1441 atgcgctttt taat
//
LOCUS       DASJOX010000238         1451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJOX010000238 DASJOX010000000
VERSION     DASJOX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.246"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            105..1406
                     /locus_tag="U4A12_002857"
     CDS             105..1406
                     /locus_tag="U4A12_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1775168.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DASJOX010000239         1451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJOX010000239 DASJOX010000000
VERSION     DASJOX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.247"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..625)
                     /locus_tag="U4A12_002858"
     CDS             complement(<1..625)
                     /locus_tag="U4A12_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1775169.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="U4A12_002859"
     CDS             complement(784..1050)
                     /locus_tag="U4A12_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1775170.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="U4A12_002860"
     CDS             complement(1040..1396)
                     /locus_tag="U4A12_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1775171.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DASJOX010000240         1445 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJOX010000240 DASJOX010000000
VERSION     DASJOX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.248"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(933..1346)
                     /locus_tag="U4A12_002861"
     CDS             complement(933..1346)
                     /locus_tag="U4A12_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN1775172.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          532 a          237 c          215 g          461 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg ttagctttgt
     1441 gaata
//
LOCUS       DASJOX010000241         1435 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJOX010000241 DASJOX010000000
VERSION     DASJOX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.249"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..989
                     /locus_tag="U4A12_002862"
     CDS             117..989
                     /locus_tag="U4A12_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1775173.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1285..>1435
                     /locus_tag="U4A12_002863"
     CDS             1285..>1435
                     /locus_tag="U4A12_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HEN1775174.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGT"
BASE COUNT          449 a          269 c          320 g          397 t
ORIGIN      
        1 aaaaagaaaa aatagtagtt atttacttgt catgttacta aataaaaaaa taatttaaat
       61 atgatatata gtcatttatt tagtgatttc attctaaaat aaagggagaa atgtctatgc
      121 tatacggtgc aattgaagca ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa
      181 tgacgatcaa ggaaagagtg agctttccga caacgacacc tgaggaaaca atgcctttag
      241 tcattgactt cttcaaacaa taccaagcag atttagcagg gattgggatt ggctcatttg
      301 ggcctatcga tatccatcgc gattccgcca cttatgggta cattacctcg accccgaaat
      361 tggcatggca aaattttgat ttcatcggta ccatgaaaaa agaatttccg atcccgattt
      421 cttggacaac agatgtcaat gcggctgcct atggtgagta tgtcttcggc agtggcaaag
      481 gattgagcag tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt gccttacaag
      541 aagggcgctt tatcgaaggg tttagtcatc cagaaatggg gcatatgctc gtcgtccctc
      601 atccaaaaga tgactttgct ggcagctgtc catttcatgg caactgttta gaagggatgg
      661 ccgctggacc cgccatcgaa aaacgcttag ggaaaaaagg acaagaggtt gcagaagatg
      721 atccttattg ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat acaaccttga
      781 tgttttctcc agatgtgatc atttttggcg gtggtgtgat gaaacaacgc catatgctgc
      841 aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa
      901 gcaaatatat cgtgacccca gcattagaag acaacgctgg aacgctcggt tgtttggcat
      961 tggctcgaga agcggtctta cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg
     1021 gtagagaaag atacaactga aaatttagtc ataaccagtt aggcagagtg cttaactggt
     1081 ttttgtttta cttggaagat caagccttga atgagataaa ttcttgctcg aaagttatgg
     1141 aaatttcgat agccgtaggc cacttgtatt gtgcaatagt tttttacacg ccgtttgtat
     1201 aattaagcga gacaaataaa aaagccattt atgttacact caaatcgtaa tacccgctaa
     1261 agcatacaag gagagtgaac ataaatgact tatacccatc ttacaccaaa cgagcttgta
     1321 atgatagaag catattttca tcaagaaact ccggttgcta tcgttgcgaa gcagcttaaa
     1381 cgtggacgcc aaacaattta caatgtctat aactttctca aatgtggtgg aacag
//
LOCUS       DASJOX010000242         1424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJOX010000242 DASJOX010000000
VERSION     DASJOX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.251"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..276)
                     /locus_tag="U4A12_002864"
     CDS             complement(<1..276)
                     /locus_tag="U4A12_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN1775175.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAI"
     gene            401..1186
                     /locus_tag="U4A12_002865"
     CDS             401..1186
                     /locus_tag="U4A12_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEN1775176.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1179..1421
                     /locus_tag="U4A12_002866"
     CDS             1179..1421
                     /locus_tag="U4A12_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775177.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          534 a          231 c          216 g          443 t
ORIGIN      
        1 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DASJOX010000243         1417 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJOX010000243 DASJOX010000000
VERSION     DASJOX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.252"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4A12_002867"
     CDS             complement(150..1337)
                     /locus_tag="U4A12_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN1775178.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DASJOX010000244         1398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJOX010000244 DASJOX010000000
VERSION     DASJOX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.253"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..>1398)
                     /locus_tag="U4A12_002868"
     CDS             complement(59..>1398)
                     /locus_tag="U4A12_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1775179.1"
                     /translation="NTSSVKDDPQASFRLSQRIPDELLQEIVQANKLYREDLSEPAKS
                     IAVMEDLIAKDRVDGNAYNRESLLQIFGKYNIQLDSDFDMEKLQEELKSIKGQPLAVY
                     TITMLFKRMKLGFDRFKGRKQPKATALFTGSTGVGKTAMAKAVARGLYGDENKVLLVN
                     MPDYKTKDDAMRFRRLIGTHVRHDPSAIIVCDEYEKAGVETLDAFLSILDEGRISFTE
                     RGWDDREVHDTVSLSNTILFATTNAGAEMFDSLNRYSSLSRVTNKENSNEYSTELKRE
                     YRKVASGIKQALIGEGLKPEVINRFGRIVPFWALSEQTLLEIAYNEMLKSAELVRKHL
                     NIDLRLPPRKERVYGQNFYADDLSMHLVFDMASQEDSKSGGARNILHLVESEFEEAIT
                     EAIITYPNAKIFDVKLSEAGAFEGKGDSNAEIIAKPSVAESKENKIVFSIGGA"
BASE COUNT          404 a          298 c          212 g          484 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtattta
//
LOCUS       DASJOX010000245         1368 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJOX010000245 DASJOX010000000
VERSION     DASJOX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.254"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1368
                     /locus_tag="U4A12_002869"
     CDS             82..>1368
                     /locus_tag="U4A12_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN1775180.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DASJOX010000246         1334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJOX010000246 DASJOX010000000
VERSION     DASJOX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.257"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            245..733
                     /locus_tag="U4A12_002870"
     CDS             245..733
                     /locus_tag="U4A12_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1775181.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            720..>1334
                     /locus_tag="U4A12_002871"
     CDS             720..>1334
                     /locus_tag="U4A12_002871"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1775182.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQ"
BASE COUNT          500 a          123 c          216 g          495 t
ORIGIN      
        1 cttattcttt tgaatctaag ataaaaattc aaaagaataa cataacccaa atatgattat
       61 acttgagatt ctaaatattt atcgaaaagg aatttgtttt aaggaaaaat gattctatgg
      121 acggttattg atttatattt accaaatggc taataaaata gtatttctta tattgtatag
      181 tttttgtttt tatgtgaaaa catgagcaat agaattgtac tagtaggata aggatggatg
      241 gaaaatgaaa aaaactattt ttgttttagc gatttttata tgttttgggc tacttttggt
      301 aggctacttg ttgtttaatt ctaatgaaat gagaactatt tctacgaatg acttgttaaa
      361 aaaaagtgaa ttaaaggata aaaaaaaaga atctcttgcc tttataatgt ttactcaaga
      421 agagtgtgag tattgtatag caataaatcc aatcatagat agtgtaaata aggaaacttt
      481 gaataagata aagaaatatg atatatctgt agattttcaa tctaaacaaa agtgtaaaat
      541 taagtttgtt ccgactattg ttgcatatag taagaataaa gaagtgggga gaattgacaa
      601 tgaattagat aatttgtatc gaaaaaagca agatggttca attgtaatag acaagaaaaa
      661 aacaaagaca ttgcttatag attggatgaa aaaaatggat aaacaaggag aatatttgta
      721 tggagaagat taaacagaca tttccgttaa ttaataaata ttggaataag aaaaaaattt
      781 ttatattttt tattacgttt ctgctgtcat ctttattttc tctaattttc ccgtatctat
      841 ttagtaatgt tattgattta tatttagaga atgagttatc acagaatatt gtgcgaatag
      901 taacgattct tatatttcag ttatttttag tcttaattaa agaagtgtat ggatatataa
      961 aaactattta tgcatttgaa gttgatttat caatgaaaat tgattttctt aaggttttac
     1021 aaaaaaaaaa tctttttttt tcgcaaacga agcattcagg agaaattcaa tttaggatgt
     1081 tttcggatat ttctactatc gtaaatacta tctttatttt ttctgttgaa ttacctgtga
     1141 taggcgtttt agttcttgta gcggtattta ttataagtaa agtgaatctt atttttctga
     1201 ttttttcact aataatgatg ggagctatgg tgttacagta taatttattt caattaccgc
     1261 ttaagaaaac aatcgagaat acaaaaagtg ttaatcaaag tattacagga gaattgaatg
     1321 cacattttga tcaa
//
LOCUS       DASJOX010000247         1330 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJOX010000247 DASJOX010000000
VERSION     DASJOX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.258"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..1121
                     /locus_tag="U4A12_002872"
     CDS             9..1121
                     /locus_tag="U4A12_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1775183.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          461 a          252 c          219 g          398 t
ORIGIN      
        1 tcgttgtcgt gttgtttagc cttagtgcgt tctctacagg aactattctt ggatggcaat
       61 tttacaataa tgaacactct cagatagttg cccatgctca atcagctcaa aaaaagttga
      121 tactgactac caatgaacaa cagctcgttg aaaaaatcga tcaactatta agaaaaaatg
      181 attatattgg ctcaatttac gtaaagaaaa ataatcgcat catcatggaa aaaggctatg
      241 gctatgcgaa taaactcacg caacaaccaa atgatccttc tctttattat cagattggtt
      301 cggttcaaaa agcattgact actcttttga ttttaaaaca agtcgagcgt gggcaccttt
      361 ctttagatac taagctggct caattctatc cacaaattgc tggcagccag caaattacta
      421 tccgtgactt gctgtatatg cgctcaggct tgcaccgaac tgcttctcca accataccga
      481 tgactgatga agaagtcgtt caatttgcga ttcagcactt aaaatttaca gattatcaga
      541 cctctcatta tgattcattg aactttacca tattgactgg aattttgatc aaattaacaa
      601 atcaaccgta tgaaaaatta ttgaccgatg atatcatcga acctttagga ttacagcata
      661 caaaatttta tgaccaagtc aaaaattcat ccaatcatgc actatcctat caaatgtcta
      721 aagaggatga ttactttaaa ccactgacag aatctgaaac agagatccat aacgaattag
      781 ggactggtaa tatcagtatg tccgtctatg atttaaatac gctgtttaca caagttttat
      841 ctggaaaact agtttccaaa gacttactct tctcattatg gcgtgaaaat gcgaatggac
      901 atccatatag tggtggggta tatagcggag ataattatat cctgtcgcaa ggaaatatcg
      961 actcttttga tacatctgct atatttagca aagattcaca ggatgctatc atcatggaaa
     1021 gcaatataaa agctgacaaa actatcaaac tgcctactgc tgatctaaga aaccaaatct
     1081 atgacctgat ggaaggaact actaagttgg caaatagctg atatgatgaa aagatagcaa
     1141 atactacttc tttgaatagc atttgcttaa aaaaagtact cgactaaacg taggaaagag
     1201 tgtgggacaa atctaaaaat caatttttgc tacagacttt aattccaaat aaacagaaca
     1261 gaaagaggtg ttcttctttc tgttctgtcc aaattattct aagatttctc tttaccaaca
     1321 ttatttgaca
//
LOCUS       DASJOX010000248         1319 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJOX010000248 DASJOX010000000
VERSION     DASJOX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.259"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U4A12_002873"
     CDS             7..624
                     /locus_tag="U4A12_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775184.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          498 a          168 c          206 g          447 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacat
//
LOCUS       DASJOX010000249         1305 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJOX010000249 DASJOX010000000
VERSION     DASJOX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.260"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1184
                     /locus_tag="U4A12_002874"
     CDS             <1..1184
                     /locus_tag="U4A12_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1775185.1"
                     /translation="NLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGT
                     RFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIG
                     AGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESP
                     KKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQT
                     NIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGF
                     SIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIV
                     GVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIE
                     T"
BASE COUNT          522 a          152 c          211 g          420 t
ORIGIN      
        1 gtaatttaga agatcttttt tatcaaaata gaatgacact aaattctgag ataaaaaagt
       61 tatttctaga gacagaagtt gacaaaattg atattcaaaa taaaaagatt agttgtgtaa
      121 aaaaagatgg tacaagattc tctcaagaat atgacaaagt tatattagct acaggttcaa
      181 agcaaattat gctagatata ccggggaata atttagctaa tattttttcg ataaagaatt
      241 ttccaaatgc aaaagagatt tttaaagctt tggatgatag tactattaaa aaagtaggaa
      301 ttattggagc aggatatatt ggagttgaaa ttgctgaagc cataagaaaa agagggaaag
      361 aagtatatct tttcgatgtt gcagatagag tgttgtcgac ttattacgac cgtagttttt
      421 cagataaagt tgaagaaata ctatcaaaaa atggaataca tctttgtcta tcagaaagtc
      481 ctaaaaaata tttaggaaga aaaaagattg aaaaaatagt cactacaagt ggtacccaat
      541 actctatgga tatggtcgta caagctatag gatttctatc taatagtcca attggggaaa
      601 aggaactctt gagagatgtt agtggagcat atctaactaa tgaataccag caaacaaata
      661 taaaagatat atatgctatt ggggattgtg ctacaacttt ttttacgtcc attaatagaa
      721 gtagtgttga tttttctatt tctaatgctc taagaacagc ttatattgca actcaacata
      781 ttaacaatca agattttaca ccttctggtt cccaatttac aaatggcttt tctatattta
      841 attacaattt ttacgcagca ggtttgaatt taaaaacagc acgattatta agtgagtcta
      901 tagattatat agaaatagaa gatttcatta gacctgcttt tttgagaaaa aatagtcgtg
      961 taaaattaag aattagcttt gaaaaaaata caaagagaat agtaggtgta caactttgta
     1021 gtcaagaaga tttatctgga ctattatcaa tgttttctct tgctatagaa gaacaaatta
     1081 cattagacaa attgaaaatg ttagattatt tatttcatcc tagttttagc caaccctata
     1141 attttatttt aaaggcagta acaggattaa acattgaaac ataatcgtta ctaaatgaaa
     1201 tcaagaaata tactgtatta ctcaattcat tgaaaatatt tatcataatt tttaatagaa
     1261 aacactatta aagagaaaaa agagaggtaa atcaaatgaa aaaat
//
LOCUS       DASJOX010000250         1296 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJOX010000250 DASJOX010000000
VERSION     DASJOX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.261"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..1111
                     /locus_tag="U4A12_002875"
     CDS             104..1111
                     /locus_tag="U4A12_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1775186.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1123..>1296
                     /locus_tag="U4A12_002876"
     CDS             1123..>1296
                     /locus_tag="U4A12_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN1775187.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQ"
BASE COUNT          506 a          163 c          243 g          384 t
ORIGIN      
        1 tcgttacgtt ttgacatcat tattgataat tagtttagtt atatattttt taaagattaa
       61 gaatcgaatt atagatgact ataattcata ggaggagata taagtgttta aaggaaaaac
      121 aatattagta actggcggaa ctggttccat tggaagtgaa attgttcgtc agcttttaaa
      181 atatgaacca aaagctatca gaatttatag tagaggtgaa gaaaaacaat tttatatgca
      241 acaagagctc tcaaatcaca ctaatttgag atttttaatt ggtgatatca gagactctca
      301 aaggattgat tatgcatgtc gtgatgtgga tattattttt catgctgccg caatgaaaca
      361 cgttccagca agtgaatata atcctatgga agctgtaaaa acaaatgtga tgggaactca
      421 aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga
      481 taaagtagtg agtcctacta acaccatggg tgcgacaaag ttattgagtg aaaaattaat
      541 aatagctgcc aatcagtata aaggaaaaac agaaacaata ttttcttgtg taaggtttgg
      601 aaatgtcatg gggtcaagtg gatctgttat tcctcttttt agtaaacaaa taaaaaacaa
      661 taaagatatt actttgacag atgttgatat gacacgattt atgatgtcaa taccggatgc
      721 tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt ggagaaacat atgtcttaaa
      781 aatgccggca atcaatattt taactttagc tcaatcatta ataaaaatat ataatgatac
      841 acatgatgaa aaatatagag gtgaaataaa agttgtaggt atcaggccag gagaaaaatt
      901 atatgaagag ctaatgacta cagacgaaat tgaacgctca tatgaaaatg ataaaatgta
      961 tatcattcca ccacactatc atgctactga cgaactgtac aaagattatg aaaaaattaa
     1021 aatcactgca tactcttcag atgcaacaga attaatgagt ggacaggaat ttattagaat
     1081 cgcaaaagag aataatgtcg tgacttggta aggagtttta tgatgaaatc agaagtatat
     1141 gtaattgcag aaattggtgt aaaccataat ggaagtctag atttagcttt agaaagtatt
     1201 gataaagcta aagaatgtgg agctaacgcg gttaaatttc aaacatttaa aacgaataag
     1261 ctagtttcca aaaaggctcc aatggcaaaa tatcag
//
LOCUS       DASJOX010000251         1292 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJOX010000251 DASJOX010000000
VERSION     DASJOX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.262"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>1292
                     /locus_tag="U4A12_002877"
     CDS             10..>1292
                     /locus_tag="U4A12_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1775188.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          401 a          282 c          258 g          351 t
ORIGIN      
        1 tgtttcaaga tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat
       61 cttacctttc atgaagatgc ctttcgcaaa gagcgcatta atgacgaaac atgttttgtt
      121 tttgatggca cattgaccta tcaaccagag gaatgcttcc attgccacta tcaaaataag
      181 caaacgatca ttaaatgggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac
      241 tacaagacca ttgtgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact
      301 tttttagcag aagattcagt tagcgatcgt cgctgctcca ttgcccgcag agtcaaacaa
      361 gccattcttg agctattaag tgagcctatt tctatgtctt tgattgcacg tatgaagcac
      421 atttctccga ccactgtcat tcgaatactt cgcagccttc ggcctaaaac cgtttctttg
      481 aaccagccgt taccagaagt ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg
      541 ggagccatga gctttgtcat gatggacggc aagacccatc aactgattga tattgtggaa
      601 aatcggcaac tcaatccttt acgcgactat ttcttgcgct accctagaaa agtccgtgaa
      661 caagtacgtc tagtggtttc agatttctat acaccctatc gcacattggt gaaagagtgt
      721 tttccgaatg cccgaatcgt cgccgatcgc tttcatatta gccaacacat cggtcgggcc
      781 tttaccaacc accgaattca agtaatgaag acctttaaaa aaggggatcg tcggtcgaag
      841 cacttaaaga aatactggag actacttcaa aagaatgcgt gggaattgaa tggtcagcat
      901 cgctactggc gtccgtcatt tcgagatcat ctgacagaag cggagatagt cgatcggcta
      961 ttatattatg atgactcctt aaagcgaggc tacgaagtct accaggtttt tctttccgct
     1021 atcagaagac aagatgttcc tgaattcgtc gcgctcctaa aagaagacta caaggagttg
     1081 ccagagcact atcaaccagt gttcacgacc ttcaagaaat atcgaacaga aatcaaacga
     1141 gcgttgcgag ttccctattc caacggccct atagaatgtt tgaacaacca tatcaaagta
     1201 ctaaaacgaa ttgcctatgg ctttagaaac tttcaaaact accgagaacg aatcttttta
     1261 tatcgaggaa aatatttcaa gaaaactaag aa
//
LOCUS       DASJOX010000252         1287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJOX010000252 DASJOX010000000
VERSION     DASJOX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.263"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..848
                     /locus_tag="U4A12_002878"
     CDS             <1..848
                     /locus_tag="U4A12_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1775189.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DASJOX010000253         1272 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJOX010000253 DASJOX010000000
VERSION     DASJOX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.264"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            265..957
                     /locus_tag="U4A12_002879"
     CDS             265..957
                     /locus_tag="U4A12_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1775190.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          391 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc tt
//
LOCUS       DASJOX010000254         1265 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJOX010000254 DASJOX010000000
VERSION     DASJOX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.265"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..1103
                     /locus_tag="U4A12_002880"
     CDS             24..1103
                     /locus_tag="U4A12_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1775191.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          335 a          343 c          356 g          231 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg
       61 gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt
      121 ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca
      181 aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt
      241 atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact
      301 gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga
      361 tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt
      421 tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca
      481 tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc
      541 atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg
      601 ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca
      661 agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga
      721 agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg
      781 ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg
      841 cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt
      901 cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg
      961 gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg
     1021 attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta
     1081 ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg
     1141 cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat
     1201 tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca
     1261 ccatg
//
LOCUS       DASJOX010000255         1246 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJOX010000255 DASJOX010000000
VERSION     DASJOX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.266"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4A12_002881"
     CDS             200..742
                     /locus_tag="U4A12_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775192.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="U4A12_002882"
     CDS             898..>1246
                     /locus_tag="U4A12_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN1775193.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DASJOX010000256         1246 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJOX010000256 DASJOX010000000
VERSION     DASJOX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.267"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            142..567
                     /locus_tag="U4A12_002883"
     CDS             142..567
                     /locus_tag="U4A12_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775194.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            597..761
                     /locus_tag="U4A12_002884"
     CDS             597..761
                     /locus_tag="U4A12_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775195.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
BASE COUNT          401 a          207 c          214 g          424 t
ORIGIN      
        1 ctagtttgtt ataacaatgc cactctaaca aaaatgtaag cattatcctt attaaaagct
       61 ccttgacaag gactatacct tttattttat gatttttaca tagaaaacgt tttctctatt
      121 ttttgtttag aggtgatttt tatgatttgc aagaacaaga aaaaaattat tgctattatt
      181 gctagtttct ctttgatttt tagtactgca tgtactgtct tattaacaaa taatgctaat
      241 gttgaagctg caagctcttc ttacaaacat aatcatgctg gttatagatg ttacaaaatg
      301 agttattatg taagtccttc taaatgcaaa caattggctc gccaaactaa taaacttaat
      361 accgtctcag ctattgcaaa ctttattgga ctatctggat taacaccggg gattatatca
      421 ttagttttcg gatcagcagt tagtgctaat aatgtatttg ttacagccgc tagaagagga
      481 aaaggcgttc aattaaatta tattgcccat tttagtaata ccactaccta taattatagt
      541 tctaaccaaa attatgttat aaaataatta aaactttaaa ctaggaggaa tgctttatga
      601 aattaacaaa aattgctgta attttagctg ttattgctgg cattatttta tattttgtat
      661 tgcctgctaa tcaattcttc ctttactttg gaatacctgc gattttaata gtaaatggcg
      721 taagaatctt tgaacaacga aaaaaatgta gctctaccta atcgttacaa aacaacaatt
      781 tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc tagccttttt
      841 tgtattgtag agcgtgaatt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
      901 gcggttattt gcaccaaata cctccgtatt tggctagcaa tccttctaaa aaatacacct
      961 atcgatcaga gtcaaaacaa atcaattttc gagtgagcga atccgaattt gcaaagctga
     1021 agctttgcaa atgagtgtgc cggcttccgt aaaaatacat atggctataa tttgcgtttt
     1081 aaggcgtttt aaacgttctg gtatataatt ataccaaaac aactttaaca gaattaggca
     1141 gaagtcgccc atcctctata ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg
     1201 gtaactcgaa gaccgacaac taaggcgtat tgccttttta ttttgt
//
LOCUS       DASJOX010000257         1230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJOX010000257 DASJOX010000000
VERSION     DASJOX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.268"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..712
                     /locus_tag="U4A12_002885"
     CDS             <1..712
                     /locus_tag="U4A12_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN1775196.1"
                     /translation="TPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLL
                     STGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSD
                     HSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLG
                     DGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGK
                     KVKRPISADSVLLEEDVE"
     gene            709..>1230
                     /locus_tag="U4A12_002886"
     CDS             709..>1230
                     /locus_tag="U4A12_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN1775197.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          406 a          165 c          268 g          391 t
ORIGIN      
        1 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
       61 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
      121 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
      181 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
      241 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
      301 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
      361 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
      421 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
      481 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
      541 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
      601 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
      661 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
      721 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
      781 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
      841 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
      901 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
      961 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     1021 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     1081 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     1141 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     1201 tcttggagag tttttcccgt cggagctcct
//
LOCUS       DASJOX010000258         1215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJOX010000258 DASJOX010000000
VERSION     DASJOX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.269"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="U4A12_002887"
     CDS             414..947
                     /locus_tag="U4A12_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN1775198.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DASJOX010000259         1193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJOX010000259 DASJOX010000000
VERSION     DASJOX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.271"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U4A12_002888"
     CDS             <1..1076
                     /locus_tag="U4A12_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1775199.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DASJOX010000260         1179 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJOX010000260 DASJOX010000000
VERSION     DASJOX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.272"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1179
                     /locus_tag="U4A12_002889"
     CDS             <1..>1179
                     /locus_tag="U4A12_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEN1775200.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     "
BASE COUNT          390 a          231 c          230 g          328 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaa
//
LOCUS       DASJOX010000261         1177 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJOX010000261 DASJOX010000000
VERSION     DASJOX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.273"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..186
                     /locus_tag="U4A12_002890"
     CDS             <1..186
                     /locus_tag="U4A12_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1775201.1"
                     /translation="PSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQ
                     KDDKVTFYGMLVENKLL"
     gene            208..>1177
                     /locus_tag="U4A12_002891"
     CDS             208..>1177
                     /locus_tag="U4A12_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1775202.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLEC"
BASE COUNT          479 a          115 c          229 g          354 t
ORIGIN      
        1 cctagtaaca atccattatg tgacgaattg attaaaaatt taaataatat atatgatgaa
       61 aaagtatgga acttattaaa gaaagatact ttactgttta agttgacatg gaaacaatct
      121 tttaaaaagc aaaaagatga taaagttact ttttatggga tgttggtaga aaacaaatta
      181 ttataaatat tagaggtata aaaagttatg aaacacaagg taagtgtgat agtaccaatt
      241 tacaatgttg aaaaatattt aagaaaatgt gtggatagtt tattgagtca gacattaaaa
      301 gagatagaaa tcgtattagt tgatgatggt tcgccagatg cttcggggga aatagcagat
      361 gagtatcaaa aaaaatattc taatgtaaaa acaatacata gagaaaatgg ggggttagga
      421 ccggcaagaa atacaggaat tgagaatgcg acaggagaat atgtggcttt tcttgattca
      481 gatgattggg tagaaagtga tatgtatgaa aaattatact tagttgcgtc caaagaaaat
      541 gctgatatag tagttagtgg acattgtgat tttgcgaatg acaaagctat ggtgaaaaag
      601 caacatcctt tgagtgggaa tgtatatgat acgcctaata gtattaaaaa tgtacgaaaa
      661 aatttatttg gacatagtcc aaaagatcaa gaagtagagg ccttcccgat gtcagtatgt
      721 atgtctttgt ataataaaaa tctgctagat aaatatagac taagatttga aaaaatttta
      781 tctgaagata caatttttaa cttgaatgca tatgatgtcg caaataaaat tgcttttaca
      841 gaatatacag attattgtta tagaaaagaa gaacaagagt ctattacaaa aagtttatct
      901 ccaaataagc agacggaatt taaagaattt ttaataactc taaaagctat agcatcgaaa
      961 gaagatttag aatgtcaaat cagagcaaaa cgtatggcaa ttgattattg taggctttat
     1021 gtgggcttag ttgatgatgc aaatttggga attaaagaga aaagaaagta tataaaaaaa
     1081 tttattgaag atgagggatt aattcaatgt tggaagggat atcctacaaa tacgctacca
     1141 tttcaacaaa aattttttca taaaatgctt gaatgta
//
LOCUS       DASJOX010000262         1164 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJOX010000262 DASJOX010000000
VERSION     DASJOX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.274"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="U4A12_002892"
     CDS             <1..969
                     /locus_tag="U4A12_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1775203.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          207 c          232 g          331 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt accc
//
LOCUS       DASJOX010000263         1133 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJOX010000263 DASJOX010000000
VERSION     DASJOX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.275"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="U4A12_002893"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="U4A12_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN1775204.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          336 a          231 c          194 g          372 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cat
//
LOCUS       DASJOX010000264         1131 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJOX010000264 DASJOX010000000
VERSION     DASJOX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.276"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(17..1075)
                     /locus_tag="U4A12_002894"
     CDS             complement(17..1075)
                     /locus_tag="U4A12_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN1775205.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          438 a          191 c          137 g          365 t
ORIGIN      
        1 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
       61 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      121 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      181 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      241 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      301 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      361 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      421 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      481 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      541 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      601 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      661 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      721 aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg
      781 taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta
      841 tttttgaaat taatacacta attaaactta aaataataga agtatatcca aacatagctc
      901 caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa
      961 gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag
     1021 atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac accatttttt
     1081 tctttttttc ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc a
//
LOCUS       DASJOX010000265         1123 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJOX010000265 DASJOX010000000
VERSION     DASJOX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.277"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..963
                     /locus_tag="U4A12_002895"
     CDS             4..963
                     /locus_tag="U4A12_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1775206.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          386 a          214 c          221 g          302 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gat
//
LOCUS       DASJOX010000266         1123 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJOX010000266 DASJOX010000000
VERSION     DASJOX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.278"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(78..1004)
                     /gene="cysK"
                     /locus_tag="U4A12_002896"
     CDS             complement(78..1004)
                     /gene="cysK"
                     /locus_tag="U4A12_002896"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN1775207.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          298 a          266 c          177 g          382 t
ORIGIN      
        1 tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg
       61 gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca
      121 ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc
      181 gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact
      241 tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa
      301 gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct
      361 tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga
      421 tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca
      481 acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc
      541 tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct
      601 tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa
      661 atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc
      721 atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta
      781 tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct
      841 ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca
      901 agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc
      961 gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg
     1021 tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt
     1081 tgactagcaa aaaaaagaca aagatatacc atatatggta att
//
LOCUS       DASJOX010000267         1123 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJOX010000267 DASJOX010000000
VERSION     DASJOX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.279"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..492)
                     /locus_tag="U4A12_002897"
     CDS             complement(136..492)
                     /locus_tag="U4A12_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN1775208.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(506..916)
                     /gene="spx"
                     /locus_tag="U4A12_002898"
     CDS             complement(506..916)
                     /gene="spx"
                     /locus_tag="U4A12_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1775209.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          360 a          206 c          168 g          389 t
ORIGIN      
        1 tgaccacata aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat
       61 aacacgcttg atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat
      121 taaaacagtt aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc
      181 aataaataga acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt
      241 gcataaaaaa atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa
      301 agcctcagac caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc
      361 ttctgcttct tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg
      421 atggtgttct aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac
      481 tttcacttcc acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga
      541 tcagatttct ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat
      601 taaatccgat tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat
      661 attctttgaa taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct
      721 tttcgtaaat tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca
      781 agatcttttt gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt
      841 caagttgatt cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag
      901 tatatacttt tatcattttc tcactcattt ttaattataa tccaattcta cagtaatttc
      961 ttttaaaata ccaattatta gctaataata tataaaaaaa aatcaaaata actgattatt
     1021 tacgtttgaa taatgagaat ttttctatat attctctatt ttgataatag tattctcatt
     1081 ttcaaaataa ataagggagg tgtgacaaaa ttcttgtcac acc
//
LOCUS       DASJOX010000268         1116 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJOX010000268 DASJOX010000000
VERSION     DASJOX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.280"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="U4A12_002899"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="U4A12_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN1775210.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DASJOX010000269         1105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJOX010000269 DASJOX010000000
VERSION     DASJOX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.281"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="U4A12_002900"
     CDS             <1..1087
                     /locus_tag="U4A12_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1775211.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DASJOX010000270         1103 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJOX010000270 DASJOX010000000
VERSION     DASJOX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.282"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..633)
                     /locus_tag="U4A12_002901"
                     /pseudo
     CDS             complement(1..633)
                     /locus_tag="U4A12_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          340 a          190 c          190 g          383 t
ORIGIN      
        1 ggtaaaaaat aagtctgatt ccttgtctct aaaggcttcg gtcagctcat ggaaaaaagt
       61 ataggcgtcc tttaaaggcg ttgaaaatgc cagcaatcga tcaatcatca ttgcttcagt
      121 gagtagcggg tatttaggtg cacgaaaact gcgccatgtt ttgtaatcat agtgattgat
      181 tttttgacgg ttttttagaa gaaagcgcca gttctttttc actttctcgg cttcccgtcg
      241 ttgccctaac tggtgtaatc ttttcatttc ttgtacacga aaatcattga acgcctgatt
      301 caaatgtttg atgatatgaa aacaatcaat gacgactttc gcatttggta agacacgttt
      361 tgtcagttgg aagtaggcgg cgttcatatc tgtcactaaa tattccaccg cttcaggatt
      421 tgcacagcga ctaaaatagt gtgttaatcg agacaatttt cgtgtaggca aaatatcaat
      481 gagttttcca gtctcgccat ccgcacaaat aaagctcatt ttatccacaa ttgaagcgtg
      541 cgaacgaaat tcatccacca tcaatacttt tggcagggtc ctttttgacg gcttcggaag
      601 atagtttttc aactctttca atgtacgaat catataccta aaataacaat gggactgaag
      661 aatcaatttt gattcatcag tcccataaat tatagagcct atattttgcg aattgcgcta
      721 cggtttcttc ttttcttttg tctgtaatat gaatatatag atccataatg atttgaatag
      781 aagagtggcc taaacgctct tgcacatctt taatatttgc actagcttgc taaaagcaag
      841 ctagcatgtg tgtgtctaag accatgaata gtaattcgtt taagattgtt tttcctgatg
      901 attacatcta accattttcg agatttagat aattgaaggt gttcatttct ttctggtaaa
      961 aatttaaata tataactaca ttttctctac taactttgaa acactacttt tgtaatataa
     1021 aaaatatttt attaataata aaattaatca cattttatat agtgagtaat ctagtgtttt
     1081 aaaaaaataa atgatactat atg
//
LOCUS       DASJOX010000271         1095 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJOX010000271 DASJOX010000000
VERSION     DASJOX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.283"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            266..>1095
                     /locus_tag="U4A12_002902"
     CDS             266..>1095
                     /locus_tag="U4A12_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1775212.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          386 a          152 c          186 g          371 t
ORIGIN      
        1 gtataattta aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga
       61 aagtttaata agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat
      121 ctttagtgat ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta
      181 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
      241 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
      301 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
      361 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
      421 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
      481 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
      541 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
      601 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
      661 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
      721 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
      781 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
      841 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
      901 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
      961 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
     1021 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
     1081 tttcaaatta gttta
//
LOCUS       DASJOX010000272         1063 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJOX010000272 DASJOX010000000
VERSION     DASJOX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.285"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..153
                     /locus_tag="U4A12_002903"
     CDS             <1..153
                     /locus_tag="U4A12_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1775213.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="U4A12_002904"
     CDS             159..575
                     /locus_tag="U4A12_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775214.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="U4A12_002905"
     CDS             681..944
                     /locus_tag="U4A12_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1775215.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1063
                     /locus_tag="U4A12_002906"
     CDS             944..>1063
                     /locus_tag="U4A12_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1775216.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPY"
BASE COUNT          407 a          132 c          215 g          309 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tat
//
LOCUS       DASJOX010000273         1058 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJOX010000273 DASJOX010000000
VERSION     DASJOX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.286"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..992)
                     /locus_tag="U4A12_002907"
     CDS             complement(36..992)
                     /locus_tag="U4A12_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775217.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DASJOX010000274         1051 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJOX010000274 DASJOX010000000
VERSION     DASJOX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.287"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="U4A12_002908"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="U4A12_002908"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1775218.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DASJOX010000275         1010 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJOX010000275 DASJOX010000000
VERSION     DASJOX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.288"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            66..>1010
                     /locus_tag="U4A12_002909"
     CDS             66..>1010
                     /locus_tag="U4A12_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775219.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKITY
                     IMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFSTL"
BASE COUNT          320 a          140 c          138 g          412 t
ORIGIN      
        1 ctgtaaaaat atttatagtt tatgcatttg ttagtttatt atatttattt ccagcattta
       61 aaaacatgga aatatttggc ttttatgata cggcatttca cattaatcgt gcgttaagtt
      121 tagaatctat ttttagctcc cctattaatt ttgaaacgtt tagatcatat ggcatgcaag
      181 taaataattt ttacccatgg ttaactctat atcctttatt tttattgatc aagtttacta
      241 atttagcaat aggctataac ctttttcttt atattgttac gctcattact ctatttatat
      301 gtcattatgt aatgtatgaa attaccaaaa aacatgttac tagctccttt ttcgctatta
      361 tatatacaac atcttctttt aggagtgtag aaatatttct tcgaggggca atgggggaat
      421 tactcgcaat gtcaatactt cctcttattt tactcgggtt tataaagctt tacgactcaa
      481 aaaaagaatc ttgggttatg ttggcgatta gcatgacttt attaatttac actcatgttc
      541 tttctgtagc tatgactatg gtaatgatta taattgcttt aattattcaa ttttatcgga
      601 aaaagaaaat agaaatcttc ttaattatta aattaataaa agctgctata gttactttac
      661 ttctttcact agcgtttata ggtcctatga ttgagcagac attgtatcaa gatctgaata
      721 gaccttatgt ggatatatta caaaaaagag caatttatct gggtaaagag tttttgattg
      781 gaagtataga ttcagaatta ctatcatttg gtattggctt aactttgctt atttgtttaa
      841 ttatactaac atttaacttt aaaaaattag gaatcttatc taaaatcact tatataatgg
      901 gtataatatc tattctactt tgtacaaaaa tatttccatg gtttgcctta caaaatactc
      961 ctttaaatat tattcaagct ccttggcgtt ttatgatatt ttcaacattg
//
LOCUS       DASJOX010000276          997 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJOX010000276 DASJOX010000000
VERSION     DASJOX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.289"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..913)
                     /locus_tag="U4A12_002910"
     CDS             complement(26..913)
                     /locus_tag="U4A12_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1775220.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DASJOX010000277          995 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJOX010000277 DASJOX010000000
VERSION     DASJOX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.290"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(66..136)
                     /locus_tag="U4A12_002911"
     tRNA            complement(66..136)
                     /locus_tag="U4A12_002911"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Cys,seq:gca)
     gene            complement(182..253)
                     /locus_tag="U4A12_002912"
     tRNA            complement(182..253)
                     /locus_tag="U4A12_002912"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Gln,seq:ttg)
     gene            complement(265..337)
                     /locus_tag="U4A12_002913"
     tRNA            complement(265..337)
                     /locus_tag="U4A12_002913"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:His,seq:gtg)
     gene            complement(340..413)
                     /locus_tag="U4A12_002914"
     tRNA            complement(340..413)
                     /locus_tag="U4A12_002914"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Trp,seq:cca)
     gene            complement(420..500)
                     /locus_tag="U4A12_002915"
     tRNA            complement(420..500)
                     /locus_tag="U4A12_002915"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(464..466),aa:Tyr,seq:gta)
     gene            complement(502..577)
                     /locus_tag="U4A12_002916"
     tRNA            complement(502..577)
                     /locus_tag="U4A12_002916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Phe,seq:gaa)
     gene            complement(613..685)
                     /locus_tag="U4A12_002917"
     tRNA            complement(613..685)
                     /locus_tag="U4A12_002917"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Asp,seq:gtc)
     gene            complement(695..767)
                     /locus_tag="U4A12_002918"
     tRNA            complement(695..767)
                     /locus_tag="U4A12_002918"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Val,seq:tac)
     gene            complement(783..854)
                     /locus_tag="U4A12_002919"
     tRNA            complement(783..854)
                     /locus_tag="U4A12_002919"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Glu,seq:ttc)
     gene            complement(869..958)
                     /locus_tag="U4A12_002920"
     tRNA            complement(869..958)
                     /locus_tag="U4A12_002920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(922..924),aa:Ser,seq:gga)
BASE COUNT          276 a          261 c          228 g          230 t
ORIGIN      
        1 actggatttt gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt gatttattag
       61 tactaaggcg gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc
      121 acttggctat accgccatta tactattatc aatctataaa ataaagaata aattatagaa
      181 actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct
      241 tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg aatgccggag
      301 ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca gcaggaattg
      361 aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc cctataaaat
      421 ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc cgtcgcgttt
      481 agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg acacacggag
      541 cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt attaattgaa
      601 taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      661 atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat cgaaccgctg
      721 accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac attcaattaa
      781 aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc
      841 ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc cttgagacag
      901 tttggcacca tctacatgat ttccaatcat gctccttcgg cctctcggac aactctccag
      961 agtgaaagaa gctcaatact ctttctcaaa atgag
//
LOCUS       DASJOX010000278          994 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJOX010000278 DASJOX010000000
VERSION     DASJOX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.291"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>994)
                     /locus_tag="U4A12_002921"
     rRNA            complement(<1..>994)
                     /locus_tag="U4A12_002921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DASJOX010000279          986 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJOX010000279 DASJOX010000000
VERSION     DASJOX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.292"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..704
                     /locus_tag="U4A12_002922"
     CDS             <1..704
                     /locus_tag="U4A12_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1775221.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DASJOX010000280          971 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJOX010000280 DASJOX010000000
VERSION     DASJOX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.293"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            102..917
                     /gene="thiD"
                     /locus_tag="U4A12_002923"
     CDS             102..917
                     /gene="thiD"
                     /locus_tag="U4A12_002923"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN1775222.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          349 a          156 c          221 g          245 t
ORIGIN      
        1 aggtagtggt atatttactg agggatactt tgaaaaacat atgatagaaa cagtaacagt
       61 attatgaaaa atctttttat aaaaatggag gaatcgattt gatggaagat cagtatgtac
      121 aagcaatgac gatcgcagga tcagatagcg atggaagcgc agggatgcaa gcagatatgc
      181 atacattctt tacaagaaat gtttacggtg tttctgtcat gaccgcatgt gttgcaggta
      241 attcttacgg aatcggagca agtgtgacat tgccaacaga ttttatcgac aaagaatttg
      301 aactgattgc gaaggacttt caaatacgtg cagctaaaac aggtatgcta gcagattcaa
      361 agttgattga gacagttgta aaaaattacc aaaaatatga ttttggacca ttagtcgtgg
      421 atcccgtaat cgtaacgaaa catggaaatc ttttactaga agaatctgct ttgcaaagct
      481 tgaaagaaaa gcttgtccct ttggccgaag tactgacacc taatttttat gaagcagaaa
      541 agctgactgg acggacatta gcagctgaag aagattttgt gcaagcagca aaagaactgc
      601 aagatatggg tgcaaaaaac gttatgatca aaggacatca tgctaaaaat gcgcagcaag
      661 aaacagtcaa tgactacgta ttgttcgaat caggaaaatc attttggtta aatgcaccat
      721 tttatcctac cgaccggatc aacggaactg gcgattcgct atctgcatgt attgcagctg
      781 agttagcgaa aggaaaaaca gttgaacaag cgatccgatt ggcaaaagac tatgtaaacc
      841 gtgcaattcg tgaagaaata aatgtagggc acaaatatgg ccccatcaat cattgggcag
      901 ctggtgaatt agagtaaaag aaagaagttg tgaaaaagtt gccctgcatc aagtaataag
      961 aacagccaag t
//
LOCUS       DASJOX010000281          945 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJOX010000281 DASJOX010000000
VERSION     DASJOX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.294"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..356
                     /locus_tag="U4A12_002924"
     CDS             87..356
                     /locus_tag="U4A12_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1775223.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="U4A12_002925"
     CDS             349..606
                     /locus_tag="U4A12_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1775224.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          359 a          101 c          176 g          309 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg aatagaataa
      901 gcaataggtt taataatcta ttttaaattt tttgtactag tttta
//
LOCUS       DASJOX010000282          941 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJOX010000282 DASJOX010000000
VERSION     DASJOX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.295"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..482
                     /locus_tag="U4A12_002926"
                     /pseudo
     CDS             1..482
                     /locus_tag="U4A12_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            516..>941
                     /locus_tag="U4A12_002927"
     CDS             516..>941
                     /locus_tag="U4A12_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HEN1775225.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIA"
BASE COUNT          344 a          105 c          177 g          315 t
ORIGIN      
        1 ttgaactttt tcaaaatcac atttcaattg aagaaagcat gagtcaatta agagatagaa
       61 tacttaaaga gtgtgcaggt tcttctgagt ataattattt agaaagtgac gaatttatag
      121 cattaagaaa ctatgttgtt aataaaatat caccaattat tgattcgaca ggtctatgtt
      181 tattagatga attcttggcg gatatcagtc tatttataat gtttattgat gtaattgaag
      241 aaacatcaag aaattttttg ctgtctaagt tcagaaaatt tcgtatgaaa aatgataaac
      301 aaaaattatt ggcaaatgag atttggagta gtttagaagt cgaagaaagt aaacaagaac
      361 ttttgttgaa atggatactt tttcttgtaa agaagttcta tgtgaaaata ccagaaaccc
      421 attgtctgat agttatgcac ggtaaaataa cagcttctgc gatcgcaagg gaaacaaata
      481 agttgctgaa tacatatgtt tatgaggctt tcgatatgcc aattgaaggt gagactagtg
      541 acttaattaa cctaatcaac aatttttgta aaagtataga tacgacgaat ggactcattc
      601 tgctagttga tatgggatca ttggaacaaa tgtatgaaaa aattgaatcc aatgtaatag
      661 gtgatttggt aattttaaac aatgtttcta cagctttagc gattgaatgt gggattcaag
      721 tttgccaaaa aaagccaatt agtcattttt accagatgga ttttgattcg tttcaagtta
      781 aagttcagta ttataaagga ttatcacaaa agaaaaatgt aatagtatct tgtctctctg
      841 gtgaggggat ttctgaaaaa gttaaggata ttttaaaacg gtatctcgat aatcaagtgg
      901 agattttgat ttttgatttt aacgcattaa agaaaatagc a
//
LOCUS       DASJOX010000283          931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJOX010000283 DASJOX010000000
VERSION     DASJOX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.296"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>931
                     /locus_tag="U4A12_002928"
     CDS             <1..>931
                     /locus_tag="U4A12_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1775226.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DASJOX010000284          905 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJOX010000284 DASJOX010000000
VERSION     DASJOX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.297"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            265..888
                     /locus_tag="U4A12_002929"
     CDS             265..888
                     /locus_tag="U4A12_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1775227.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          334 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaaa
//
LOCUS       DASJOX010000285          826 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJOX010000285 DASJOX010000000
VERSION     DASJOX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.298"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          297 a          143 c          111 g          275 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaatttattg
      661 caaaaaaaat agaagtgaca aaattatcaa tcatttcaag gtaatataac tacctgagtt
      721 tattatcgat taaaatgcta actacatttg caagagaagc gagagttaaa ggtaaatata
      781 gaatttctac ttttttagca atagaaatag gaataaaata tttaaa
//
LOCUS       DASJOX010000286          818 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJOX010000286 DASJOX010000000
VERSION     DASJOX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.299"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>818
                     /locus_tag="U4A12_002930"
     CDS             <1..>818
                     /locus_tag="U4A12_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775228.1"
                     /translation="TLIIWGIGSNAEFENFIKLCSTLLYLILAYGYACTAFYLFKAEG
                     IDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGETNTANFAMEVHDLTFAMG
                     VFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALSGAILVYYVLLKWGKKIQ
                     IRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGY
                     VGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWL"
BASE COUNT          250 a           80 c          151 g          337 t
ORIGIN      
        1 tactctaata atatggggaa ttggttcaaa tgcagaattt gaaaatttta taaagttatg
       61 ttccacgttg ctttatttaa ttcttgcata tggttacgca tgtacagcat tttacttatt
      121 taaggcagaa ggaattgatt ttattttttg gtcaggtgtt atttcatata tgagtggttc
      181 agttttttat ttaattgtat cttatggttt aaatggttta atttcttata taaaaagtct
      241 tataacaggt gaaacaaata ctgcaaattt tgctatggag gtacatgatt taacttttgc
      301 gatgggggtt tttttccttt attacgtatt ttttgaaaat aaaaatacaa aaaaacataa
      361 gataaaaatt atcgtatctt taattttaat tttcatggga ttgaagagaa ttgaactgtt
      421 agctttaagt ggtgcaattc tagtgtatta tgttttatta aagtggggaa aaaagattca
      481 aatccgtgca ttagtgtgta ctatttgtgc aactgttatt tcgttagtgt ttatttacat
      541 tatagataca ggaattttgg cattgcttat gtcaaatttg ggcattacag atgacggtgc
      601 gaggctttca ttttatctat attcatctaa attttatgaa cttggtttag gatatgtagg
      661 aacaggatgg acatggtttt caaaatactt ttttaatctt tatttatcgc actttaggtt
      721 aaatggtgga agaattgcag caagcctaca cagtgatata ttaactattt atatagaagt
      781 tggatgtata gtttttatta tatggatgtt ttggttat
//
LOCUS       DASJOX010000287          809 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJOX010000287 DASJOX010000000
VERSION     DASJOX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.300"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>809
                     /locus_tag="U4A12_002931"
     CDS             <1..>809
                     /locus_tag="U4A12_002931"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1775229.1"
                     /translation="LWIGGIFLIDVILLAKGTISITNFVIVFMYANMVPTLWTTLISI
                     IWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNISFST
                     EMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISIATQ
                     IPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKILLSG
                     GEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYII"
BASE COUNT          339 a           83 c          125 g          262 t
ORIGIN      
        1 ttatggattg gaggaatatt tcttattgat gttattttat tagctaaagg aacaataagt
       61 attactaatt ttgtaatagt atttatgtac gctaacatgg tccctacttt atggactact
      121 ttaattagta ttatttggaa atggcaagac tattcagtgt cattaaatag atattacgaa
      181 tattattcaa ataacaacaa agtggctggc acaaaaaaaa taaataaaat aaattctatt
      241 actggtcaat cattaaattt ttcatataac aaaagaaagg tattaagcaa tataagtttt
      301 tcaactgaaa tgaataaact attcatgata tcaggagaaa atggaacagg gaaaacaaca
      361 ctgatgaaat tatttactaa acagttgccg gttagtaata atcaattatt cgtcaacggg
      421 atagatatta atatgattga taacgaatca ttaagaaggt taatttcaat cgcaacacaa
      481 ataccatata taattaatag ctccgtgaag gagaatattt taatggggac taaaaactat
      541 gataaaaaaa aattcagtaa attaatagaa ctattgggaa taagtaaatt tatgacgaaa
      601 ttaggtatta ctttggacga caatatttat gatgaaaaaa tattattatc tggaggagaa
      661 aaacaaaaaa taagtttagc tagggcgctt attaaatcag cttcagttat tatattagat
      721 gaaccattta gaaatttaga taatgaaact cgcgataatt tgagggatta tatactaaat
      781 acaaaaaaag aagtttgtta tataattat
//
LOCUS       DASJOX010000288          733 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJOX010000288 DASJOX010000000
VERSION     DASJOX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.302"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>733
                     /locus_tag="U4A12_002932"
     rRNA            <1..>733
                     /locus_tag="U4A12_002932"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DASJOX010000289          732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJOX010000289 DASJOX010000000
VERSION     DASJOX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.303"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..>732)
                     /locus_tag="U4A12_002933"
     CDS             complement(109..>732)
                     /locus_tag="U4A12_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEN1775230.1"
                     /translation="STYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYV
                     RVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKE
                     FDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLEL
                     FDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          207 a          166 c          138 g          221 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta ga
//
LOCUS       DASJOX010000290          713 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJOX010000290 DASJOX010000000
VERSION     DASJOX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.304"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            103..471
                     /locus_tag="U4A12_002934"
     CDS             103..471
                     /locus_tag="U4A12_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN1775231.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          254 a          117 c          108 g          234 t
ORIGIN      
        1 aatatagcgg ttacaacatt acttgtgttt tgttcgtttt ttatacataa ctattgtttt
       61 tctggtaaaa taaatcaaac cacttaatgg aggcgtttta gaatggaaaa atttcagccg
      121 atcatcaatc aaatcaaaga aaaagaacat caacaaaaga tcaaagacct ttttgctaag
      181 ataacagaaa cgtatcccca gctagacaca actgtaaaat ggaaccaacc tatgtttact
      241 gaccatggta cctttattat tgcatttagt tttgctaaaa atcatttttc tatcgcaccg
      301 gaaaaagcag cgattcgtgc ccttgaaaaa aatatccaag aagcaggcta cgtatatacg
      361 gataacgtca tcaaagttcc ttggaaaagt gtaatcaact gggaattgat tgaacaattg
      421 atttcattta atattgaaga taaaaaaggt catgataaat tttggcgata aagctttata
      481 cgaataaacc ttttactggc gaggaaacta ttcatcacct tatacaggtt ttgttcaatc
      541 ctatcctttt tatcatccct ggttaaacac ttaattgaca aagtatctgc tatttttata
      601 aggagtcttt cttgtgctga aaaaaatcac tgattgaatg gctttgaatt ttctacatag
      661 taaaattatt ttttcagcaa ttttcctgca aaacttttga ataaggaaaa aat
//
LOCUS       DASJOX010000291          702 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJOX010000291 DASJOX010000000
VERSION     DASJOX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.305"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..436)
                     /locus_tag="U4A12_002935"
     CDS             complement(<1..436)
                     /locus_tag="U4A12_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1775232.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          214 a          120 c           95 g          273 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc atttttatta
      661 ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DASJOX010000292          685 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJOX010000292 DASJOX010000000
VERSION     DASJOX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.306"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(156..>685)
                     /locus_tag="U4A12_002936"
     CDS             complement(156..>685)
                     /locus_tag="U4A12_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1775233.1"
                     /translation="WHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVM
                     TQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPD
                     SLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFE
                     KDKYIINLINDIEVN"
BASE COUNT          229 a          125 c           71 g          260 t
ORIGIN      
        1 attttgaata tacgttattt cttcaaactc tttttttcca ctgctactat aagctgttat
       61 atcaaaatta atagaattct tttttccata gcttattaga ttttttagat gtctctttcc
      121 aattgaccct aacccaaaaa aaattacttt catcattaat ttacctcaat gtcgttaatt
      181 aagttaataa tatacttatc tttttcaaaa gtatattggg gttccatttt cttttccaaa
      241 agcgtgaaaa attctttaat ctcctctaaa tatgcatctt caataatagt atgtgaataa
      301 ttattatcat gcataacctc atcgtacaat tgaatatctg tcatactaga agtttcttca
      361 ttccatgaaa atagagaatc cgggttacct tcccattcta ttaaaatctt ttcaccaatt
      421 atagttaaat ttcttttagc tactcttgaa acaacatcta cacttaaagt tccaactata
      481 ccagtttcat gctcaataat aagagtgaaa ctgtccgaat aatcaatatc aagattggat
      541 aatttctggg tcattacagt aaacgattta actttcccaa atgtatctat aatccaaggt
      601 aattcaatag cgaatatttc tctacaccca ttagtttttt tgtttgaaac aaaaaaattg
      661 cgataatctt cccaaggatg ccagt
//
LOCUS       DASJOX010000293          679 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJOX010000293 DASJOX010000000
VERSION     DASJOX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.307"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..>679
                     /locus_tag="U4A12_002937"
     CDS             34..>679
                     /locus_tag="U4A12_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1775234.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYR"
BASE COUNT          253 a           73 c          127 g          226 t
ORIGIN      
        1 aaatattata tttaaataag gagtatgata ataatgaata gcaaaaaact tcagatgggt
       61 gaaattgtta gaggtcctag tcaaatatct attgatgtta catataaatg taattttaga
      121 tgtaaacatt gttacaatgc gagtggtgga gacaattgtt ttatggataa tgagttgact
      181 gatgatgaac tgttagaact tacaaaagat atatgtaatt taaagccata taatatttgt
      241 ttctgtggag gagaaacttt actgagaaaa aaaatagttt tatcttgtgc cactttatta
      301 aaagaggcag gtattcctaa tattgctatt gttactaatg gatcattagt aactcaggat
      361 gttgcaaaag aattattcaa agcaggtatt aatcgatttc aagtaagttt agatggaaaa
      421 aatgctagta gccatgaagc acttagatta tatccaaaca gttttgaaaa ggcaattaat
      481 gctatcaaat tatttagaga attgtccgaa gaagtggatg ttgctttttg tcctactagt
      541 tttaattaca atcaattacg agaagtatat caactagtaa atactcttaa agtgaagtct
      601 ttaagaattc aaccatttat gcttagtggt aggggaatgc aaaataggga aatagtgcca
      661 actgtttctc aatatagaa
//
LOCUS       DASJOX010000294          632 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJOX010000294 DASJOX010000000
VERSION     DASJOX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.308"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..271
                     /locus_tag="U4A12_002938"
     CDS             68..271
                     /locus_tag="U4A12_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEN1775235.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            271..621
                     /locus_tag="U4A12_002939"
     CDS             271..621
                     /locus_tag="U4A12_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1775236.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          261 a           66 c          112 g          193 t
ORIGIN      
        1 attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa
       61 gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg
      121 tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga
      181 aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa
      241 tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa
      301 aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta
      361 ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct
      421 gaaattcttg taaataaatt agttaattat atttacatcg tgggttttga taataaaata
      481 cgctttattg ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct
      541 ggaattaata gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca
      601 gaaacaatgc ctcaacgata aaatatagta ta
//
LOCUS       DASJOX010000295          618 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJOX010000295 DASJOX010000000
VERSION     DASJOX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.309"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..437)
                     /locus_tag="U4A12_002940"
     CDS             complement(<1..437)
                     /locus_tag="U4A12_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775237.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGIS"
     gene            403..>618
                     /locus_tag="U4A12_002941"
     CDS             403..>618
                     /locus_tag="U4A12_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775238.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIF"
BASE COUNT          227 a           82 c           82 g          227 t
ORIGIN      
        1 gaaattccat aaaaaacgac tgcaggaatt attgtaagaa aaggataaaa atagttcagt
       61 ccatctccat aattgttaaa tgttgtatag ttgattggcc cttcaaaaat agaagataac
      121 cctttgattc gtgaaagatg aaaagataaa tcacctgttg tataaatttc ttgagcattt
      181 ctcataaaat ttactgaata taaagtacta agtaagataa gaactactaa aagaaatgct
      241 tttttactta ttttcataga tttgttgttc tcctttttta attctatttt cagtatacta
      301 gaattgtctt tttttaataa gttttgcgtt aaatttttca tagcactagt atgatatact
      361 agaaaagaat caatttttat tacttttaaa taggagaaaa aaatgaaaaa aaacaaaaac
      421 caaataatag atatcatttt tatgttattt ctaagttttg cttacgtcat ccctctattc
      481 atgtctaagg gaatctatca tagtgtcaat caggatactt actttcattt atctcgaatc
      541 ataggactag ataatgtatg gagttctcca gtaaacttca ataacttcga tcatcatgga
      601 acaatgatga atattttt
//
LOCUS       DASJOX010000296          580 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJOX010000296 DASJOX010000000
VERSION     DASJOX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.310"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          190 a           89 c           90 g          211 t
ORIGIN      
        1 ttctgtgtta gaattttttt gaagagtatt atacaagcta aagcttttct tcattgccac
       61 tcaaatgagt ggctttttta tgtatccttt tatggattaa tgaaaggatg tttcacatag
      121 ttatacttct gtatatttga aaagttttac tttgattttt aaatagaaag acatttgggt
      181 taaattgtga gataataata aagaagagtt taaagcgcac cccaaaccac ttccccataa
      241 gtgtgttacg ctttaaactc ttttatattt gaagccatta aaaagcatac catatttttg
      301 aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt taattagtga cttatttttg
      361 attttatagc actgatacta taaaatatag atatcatcat attacacaat cttaatacta
      421 acttaaaaaa tatctccttt cacaagtatg gtgataaaat tcgttccggg ctaccttttt
      481 aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact ttatatagtc
      541 ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc
//
LOCUS       DASJOX010000297          561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJOX010000297 DASJOX010000000
VERSION     DASJOX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.311"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..454
                     /locus_tag="U4A12_002942"
     CDS             <1..454
                     /locus_tag="U4A12_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1775239.1"
                     /translation="DLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLE
                     LFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          171 a          107 c          121 g          162 t
ORIGIN      
        1 ggatctgttc aatcgagaaa ttctcggcta ttcttgtgga gaacataaag atgccgttct
       61 agtaaaaaaa gcatttagcc gtatcaaaca acctctgaca gaggttgaga tttttcatac
      121 tgatcgtgga aaagagtttg ataaccaagc tattgatgaa ttattaacaa cttttgacat
      181 caatcgatca ttgagtcata aaggctgtcc ttttgataat gccgtagctg aatcaactta
      241 taagtcgttg aaagtagaat ttgtctatca atacacattt gaaaccttac aacaattgga
      301 tttggagtta tttgactatg tcaattggtg gaaccacctt cggttgcacg gtacacttgg
      361 ctacgagaca ccggttggtt accgtaacca gagattggcg cagcgaatcc ttgataatga
      421 gctcggatgt gctaacgcta gcgaggcagt ctaactttaa ctgttagctc ctgccgaaga
      481 tcgtcacatc cgaggaggct cattgtcaag gacaatcgga atagcatacg gaaagaagtt
      541 ctgcacctta taaaatttgt c
//
LOCUS       DASJOX010000298          546 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJOX010000298 DASJOX010000000
VERSION     DASJOX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.312"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..516
                     /locus_tag="U4A12_002943"
     CDS             <1..516
                     /locus_tag="U4A12_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN1775240.1"
                     /translation="KLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLT
                     LDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVC
                     TEIKVLLGIPA"
BASE COUNT          197 a           97 c          104 g          148 t
ORIGIN      
        1 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
       61 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      121 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      181 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      241 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      301 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      361 cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg catggaagcc
      421 attcgaggat tatataagaa aacccgaaaa gaaggcactc tcttcgggtt ttcggtctgt
      481 actgaaatca aggtattatt gggaatccca gcttaaatca tagataccgt aagggatttt
      541 attctt
//
LOCUS       DASJOX010000299          526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJOX010000299 DASJOX010000000
VERSION     DASJOX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.314"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="U4A12_002944"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="U4A12_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN1775241.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="U4A12_002945"
     CDS             complement(349..>526)
                     /locus_tag="U4A12_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775242.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DASJOX010000300          515 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJOX010000300 DASJOX010000000
VERSION     DASJOX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.315"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..>515)
                     /locus_tag="U4A12_002946"
     CDS             complement(107..>515)
                     /locus_tag="U4A12_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN1775243.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DASJOX010000301          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJOX010000301 DASJOX010000000
VERSION     DASJOX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.316"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U4A12_002947"
     CDS             complement(<1..132)
                     /locus_tag="U4A12_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1775244.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DASJOX010000302          510 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJOX010000302 DASJOX010000000
VERSION     DASJOX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.317"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>510
                     /locus_tag="U4A12_002948"
     CDS             <1..>510
                     /locus_tag="U4A12_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775245.1"
                     /translation="KFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLL
                     VNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENS
                     QWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEK
                     RIDNIINYI"
BASE COUNT          209 a           55 c           92 g          154 t
ORIGIN      
        1 ctaaatttgc aaactcattc tggtttttaa aaaatattaa taaaacagtg gaaaagaatg
       61 gagtttataa aggtgatttt cattttcaaa atgctgagac tattgaacga gaacatcatt
      121 atttcgattt attagtgaat acagtggtag aattagcgaa atgggcaaag tcaaacgaaa
      181 cttatagtca agtacttaaa agaattatga gtcaagaaac ttcatttatc tatgagttaa
      241 gcaagtcaaa tttgaaatat acttataatg aagatgaact tgaaatttta gataatccag
      301 aaaattcaca atggattaaa gagttgaaga gagaagactt tattgagaca ctaagaattc
      361 taaaatcgtt atttgatttg aaattagcca aagctaccat catgggaatt gaaagagatt
      421 catattattg ggaggaaatt ggactatatg ctccccagat tgaaggtgaa gataaagaga
      481 aaaggataga caacattatt aactatatta
//
LOCUS       DASJOX010000303          509 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJOX010000303 DASJOX010000000
VERSION     DASJOX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.318"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..425)
                     /gene="spxA"
                     /locus_tag="U4A12_002949"
     CDS             complement(27..425)
                     /gene="spxA"
                     /locus_tag="U4A12_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1775246.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          146 a          104 c           85 g          174 t
ORIGIN      
        1 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
       61 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
      121 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
      181 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
      241 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
      301 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
      361 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
      421 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
      481 taatctccca tctgtttcta ttatacata
//
LOCUS       DASJOX010000304          508 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJOX010000304 DASJOX010000000
VERSION     DASJOX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.319"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DASJOX010000305          484 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJOX010000305 DASJOX010000000
VERSION     DASJOX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.320"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..258)
                     /locus_tag="U4A12_002950"
     CDS             complement(61..258)
                     /locus_tag="U4A12_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1775247.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DASJOX010000306          474 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJOX010000306 DASJOX010000000
VERSION     DASJOX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.321"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..>474)
                     /locus_tag="U4A12_002951"
     CDS             complement(45..>474)
                     /locus_tag="U4A12_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1775248.1"
                     /translation="NNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGK
                     TALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLN
                     KENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          167 a           72 c           24 g          211 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attc
//
LOCUS       DASJOX010000307          473 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJOX010000307 DASJOX010000000
VERSION     DASJOX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.322"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>473
                     /locus_tag="U4A12_002952"
     CDS             <1..>473
                     /locus_tag="U4A12_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775249.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           71 c          112 g           98 t
ORIGIN      
        1 accgcaggag aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc
       61 gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
      121 aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
      181 gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg
      241 actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta
      301 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa
      361 aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt
      421 acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DASJOX010000308          462 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.323, whole genome shotgun sequence.
ACCESSION   DASJOX010000308 DASJOX010000000
VERSION     DASJOX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.323"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="U4A12_002953"
     CDS             complement(<1..430)
                     /locus_tag="U4A12_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1775250.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DASJOX010000309          452 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.324, whole genome shotgun sequence.
ACCESSION   DASJOX010000309 DASJOX010000000
VERSION     DASJOX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.324"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>452
                     /locus_tag="U4A12_002954"
     CDS             <1..>452
                     /locus_tag="U4A12_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775251.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DASJOX010000310          429 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.325, whole genome shotgun sequence.
ACCESSION   DASJOX010000310 DASJOX010000000
VERSION     DASJOX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.325"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>429
                     /locus_tag="U4A12_002955"
     CDS             <1..>429
                     /locus_tag="U4A12_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1775252.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGT"
BASE COUNT          191 a           59 c           70 g          109 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactg
//
LOCUS       DASJOX010000311          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.326, whole genome shotgun sequence.
ACCESSION   DASJOX010000311 DASJOX010000000
VERSION     DASJOX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.326"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="U4A12_002956"
     CDS             complement(<1..339)
                     /locus_tag="U4A12_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1775253.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DASJOX010000312          400 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.327, whole genome shotgun sequence.
ACCESSION   DASJOX010000312 DASJOX010000000
VERSION     DASJOX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.327"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="U4A12_002957"
     CDS             <1..390
                     /locus_tag="U4A12_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1775254.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          156 a           72 c           60 g          112 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt
//
LOCUS       DASJOX010000313          399 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.328, whole genome shotgun sequence.
ACCESSION   DASJOX010000313 DASJOX010000000
VERSION     DASJOX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.328"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DASJOX010000314          378 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.329, whole genome shotgun sequence.
ACCESSION   DASJOX010000314 DASJOX010000000
VERSION     DASJOX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.329"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          133 a           57 c           42 g          146 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat
      361 cataatttgt ctgcacca
//
LOCUS       DASJOX010000315          345 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.331, whole genome shotgun sequence.
ACCESSION   DASJOX010000315 DASJOX010000000
VERSION     DASJOX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.331"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..321)
                     /locus_tag="U4A12_002958"
     CDS             complement(<1..321)
                     /locus_tag="U4A12_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1775255.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DASJOX010000316          338 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.332, whole genome shotgun sequence.
ACCESSION   DASJOX010000316 DASJOX010000000
VERSION     DASJOX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.332"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..338
                     /locus_tag="U4A12_002959"
                     /pseudo
     CDS             1..338
                     /locus_tag="U4A12_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DASJOX010000317          331 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.333, whole genome shotgun sequence.
ACCESSION   DASJOX010000317 DASJOX010000000
VERSION     DASJOX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.333"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>331
                     /locus_tag="U4A12_002960"
     CDS             <1..>331
                     /locus_tag="U4A12_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEN1775256.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DASJOX010000318          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.334, whole genome shotgun sequence.
ACCESSION   DASJOX010000318 DASJOX010000000
VERSION     DASJOX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.334"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4A12_002961"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4A12_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN1775257.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DASJOX010000319          321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.335, whole genome shotgun sequence.
ACCESSION   DASJOX010000319 DASJOX010000000
VERSION     DASJOX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.335"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..321)
                     /locus_tag="U4A12_002962"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="U4A12_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DASJOX010000320          315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.336, whole genome shotgun sequence.
ACCESSION   DASJOX010000320 DASJOX010000000
VERSION     DASJOX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.336"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>315
                     /locus_tag="U4A12_002963"
     CDS             <1..>315
                     /locus_tag="U4A12_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775258.1"
                     /translation="TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIY
                     SSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESG
                     INK"
BASE COUNT          130 a           38 c           49 g           98 t
ORIGIN      
        1 acttattcta atgaacaaat ggaaaataaa cttgaagaat ttgagaattt tgattatgta
       61 cctataggct ataaaaaaga tcaattgctt ttgaaaaacc atcaagttac agaagatggt
      121 caagttattt atagtagctt ttctccaaat aaagatgaaa ttacatttac gtttaaatct
      181 tcaaaaggca aaacaatagt tttacctgta ttccggtata aaggattaac ggttactgtt
      241 aattcaaaaa atgctacagt actaaaacaa ggaggtcctt ttgttacagt aaaaagtgaa
      301 agtggaataa ataaa
//
LOCUS       DASJOX010000321          309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.337, whole genome shotgun sequence.
ACCESSION   DASJOX010000321 DASJOX010000000
VERSION     DASJOX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.337"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..309
                     /locus_tag="U4A12_002964"
                     /pseudo
     CDS             1..309
                     /locus_tag="U4A12_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DASJOX010000322          308 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.338, whole genome shotgun sequence.
ACCESSION   DASJOX010000322 DASJOX010000000
VERSION     DASJOX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.338"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..>308)
                     /locus_tag="U4A12_002965"
     CDS             complement(76..>308)
                     /locus_tag="U4A12_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1775259.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DASJOX010000323          297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.341, whole genome shotgun sequence.
ACCESSION   DASJOX010000323 DASJOX010000000
VERSION     DASJOX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.341"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>297
                     /locus_tag="U4A12_002966"
     rRNA            <1..>297
                     /locus_tag="U4A12_002966"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           92 a           61 c           76 g           68 t
ORIGIN      
        1 tcgggttacc gaattcagat aaactccgaa tgccattcat tcatatccgg gagtcagact
       61 gtgagtgata agatccatag tcgaaaggga aacagcccag accaccagct aaggtcccaa
      121 aatatatgtt aagtggaaaa ggatgtgggg ttgcacagac aactaggatg ttggcttaga
      181 agcagccacc atttaaagag tgcgtaatag ctcactagtc gagtgaccct gcgccgaaaa
      241 tgtaccgggg ctaaacatat taccgaagct gtggagtaca cctttaggtg tattggt
//
LOCUS       DASJOX010000324          282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.342, whole genome shotgun sequence.
ACCESSION   DASJOX010000324 DASJOX010000000
VERSION     DASJOX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.342"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>282
                     /locus_tag="U4A12_002967"
     CDS             <1..>282
                     /locus_tag="U4A12_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEN1775260.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DASJOX010000325          270 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.343, whole genome shotgun sequence.
ACCESSION   DASJOX010000325 DASJOX010000000
VERSION     DASJOX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.343"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..268
                     /locus_tag="U4A12_002968"
     CDS             <1..268
                     /locus_tag="U4A12_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1775261.1"
                     /translation="YQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFY
                     REKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          116 a           28 c           43 g           83 t
ORIGIN      
        1 gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat aacattattc aaagcatgtc
       61 tagaaaagga aattgcttag ataattctgt aatagaaaat ttttttggtg tgcttaaatc
      121 agagtttttt tatcgagaaa aattccgatc aattgagata tttcaaagta aattaaatga
      181 gtatattagg tggtataata acaaaaggat aaaactaaag ttaaatggac tatctccagt
      241 agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DASJOX010000326          265 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.344, whole genome shotgun sequence.
ACCESSION   DASJOX010000326 DASJOX010000000
VERSION     DASJOX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.344"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           94 a           42 c           37 g           92 t
ORIGIN      
        1 ctctcagaag cgttctgaga gcttttgaca ttttcttgta taattttatc ctaacacatt
       61 acaaagcacc taattcgagc ttatatcaag caaatttgct atttattact caatacatat
      121 ctaaaaatta cttgatggaa aattagatac atggtaagca tgaaaagaaa atcaatttga
      181 gcagatgcag tacaatacat agcaatttaa aagctctcag aaggcttctg agagctttta
      241 atatattctt tgataatttg tcttt
//
LOCUS       DASJOX010000327          261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.345, whole genome shotgun sequence.
ACCESSION   DASJOX010000327 DASJOX010000000
VERSION     DASJOX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.345"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>261
                     /locus_tag="U4A12_002969"
     CDS             22..>261
                     /locus_tag="U4A12_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1775262.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           98 a           45 c           49 g           69 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt c
//
LOCUS       DASJOX010000328          252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.denovo.347, whole genome shotgun sequence.
ACCESSION   DASJOX010000328 DASJOX010000000
VERSION     DASJOX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.denovo.347"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..>252)
                     /locus_tag="U4A12_002970"
     CDS             complement(32..>252)
                     /locus_tag="U4A12_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1775263.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DASJOX010000329        20420 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.044, whole genome shotgun sequence.
ACCESSION   DASJOX010000329 DASJOX010000000
VERSION     DASJOX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.044"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            265..1305
                     /locus_tag="U4A12_002971"
     CDS             265..1305
                     /locus_tag="U4A12_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1775264.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1403..1591
                     /locus_tag="U4A12_002972"
     CDS             1403..1591
                     /locus_tag="U4A12_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1775265.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1649..2002)
                     /locus_tag="U4A12_002973"
     CDS             complement(1649..2002)
                     /locus_tag="U4A12_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1775266.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2533..2955
                     /locus_tag="U4A12_002974"
     CDS             2533..2955
                     /locus_tag="U4A12_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1775267.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2952..3182
                     /locus_tag="U4A12_002975"
     CDS             2952..3182
                     /locus_tag="U4A12_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1775268.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3680..3880
                     /locus_tag="U4A12_002976"
     CDS             3680..3880
                     /locus_tag="U4A12_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1775269.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3907..5100
                     /locus_tag="U4A12_002977"
     CDS             3907..5100
                     /locus_tag="U4A12_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1775270.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5168..6733)
                     /gene="guaA"
                     /locus_tag="U4A12_002978"
     CDS             complement(5168..6733)
                     /gene="guaA"
                     /locus_tag="U4A12_002978"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1775271.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6792..7715)
                     /locus_tag="U4A12_002979"
     CDS             complement(6792..7715)
                     /locus_tag="U4A12_002979"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1775272.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7911..8516
                     /locus_tag="U4A12_002980"
     CDS             7911..8516
                     /locus_tag="U4A12_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1775273.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8824..9840
                     /locus_tag="U4A12_002981"
     CDS             8824..9840
                     /locus_tag="U4A12_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1775274.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9981..11282
                     /locus_tag="U4A12_002982"
     CDS             9981..11282
                     /locus_tag="U4A12_002982"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1775275.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11328..11990
                     /gene="deoC"
                     /locus_tag="U4A12_002983"
     CDS             11328..11990
                     /gene="deoC"
                     /locus_tag="U4A12_002983"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1775276.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12011..12409
                     /locus_tag="U4A12_002984"
     CDS             12011..12409
                     /locus_tag="U4A12_002984"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1775277.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12475..13557
                     /locus_tag="U4A12_002985"
     CDS             12475..13557
                     /locus_tag="U4A12_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1775278.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13769..15331
                     /locus_tag="U4A12_002986"
     CDS             13769..15331
                     /locus_tag="U4A12_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1775279.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15324..16457
                     /locus_tag="U4A12_002987"
     CDS             15324..16457
                     /locus_tag="U4A12_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1775280.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16457..17413
                     /locus_tag="U4A12_002988"
     CDS             16457..17413
                     /locus_tag="U4A12_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1775281.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17512..18678
                     /gene="deoB"
                     /locus_tag="U4A12_002989"
     CDS             17512..18678
                     /gene="deoB"
                     /locus_tag="U4A12_002989"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1775282.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18702..19520
                     /locus_tag="U4A12_002990"
     CDS             18702..19520
                     /locus_tag="U4A12_002990"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1775283.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19537..20244
                     /gene="deoD"
                     /locus_tag="U4A12_002991"
     CDS             19537..20244
                     /gene="deoD"
                     /locus_tag="U4A12_002991"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1775284.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6549 a         3655 c         4364 g         5852 t
ORIGIN      
        1 ttttctataa gttatattat cataatacca caaaatcaca aattttcatt caattatcat
       61 tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa
      121 aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt
      181 gcatatatac gcctttatag tggagtacta catttacgag attcggttag agtatcagaa
      241 aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt
      301 gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt
      361 gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta
      421 ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt
      481 ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa
      541 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
      601 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      661 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      721 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      781 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      841 gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta
      901 tactatagcc ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag
      961 caagccttta gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat
     1021 gcaccacagg aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc
     1081 gtaaatactc aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt
     1141 attcaagatt atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca
     1201 gagctaaaag gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat
     1261 agtcggatag ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt
     1321 tatataaata tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta
     1381 aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg
     1441 aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg
     1501 ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa
     1561 agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg
     1621 ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac
     1681 cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt
     1741 ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa
     1801 tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt
     1861 tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac
     1921 gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt
     1981 atttatcttc ttttttacgc atactaaatc acctctatac attttactgt tcctatataa
     2041 ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg
     2101 aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat
     2161 acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga
     2221 gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc
     2281 aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac
     2341 atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg
     2401 tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg
     2461 tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag
     2521 ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat
     2581 atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct agacagacta
     2641 tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact
     2701 gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt
     2761 gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt
     2821 ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat
     2881 cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag
     2941 gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga
     3001 cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa
     3061 aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa
     3121 ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat
     3181 agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt
     3241 tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac
     3301 gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa
     3361 ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc
     3421 tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata
     3481 gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt
     3541 gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt
     3601 gtattattat aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat
     3661 aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata
     3721 tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg
     3781 gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat
     3841 tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta
     3901 aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca
     3961 aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact
     4021 aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt
     4081 gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat
     4141 aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc
     4201 tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa
     4261 ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca
     4321 gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt
     4381 ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa
     4441 gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg
     4501 tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt
     4561 gattttgact caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat
     4621 tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc
     4681 acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt
     4741 gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc
     4801 tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct
     4861 tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa
     4921 gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta
     4981 gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca
     5041 ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa
     5101 aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct
     5161 cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     5221 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     5281 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     5341 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     5401 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     5461 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     5521 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     5581 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     5641 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     5701 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     5761 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     5821 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     5881 cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     5941 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     6001 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
     6061 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
     6121 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
     6181 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
     6241 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
     6301 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
     6361 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
     6421 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
     6481 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
     6541 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
     6601 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
     6661 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
     6721 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
     6781 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
     6841 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
     6901 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
     6961 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
     7021 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
     7081 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
     7141 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
     7201 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
     7261 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
     7321 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
     7381 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
     7441 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
     7501 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
     7561 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
     7621 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
     7681 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
     7741 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
     7801 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
     7861 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
     7921 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
     7981 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
     8041 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
     8101 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
     8161 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
     8221 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
     8281 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
     8341 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
     8401 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
     8461 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
     8521 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
     8581 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
     8641 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
     8701 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
     8761 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
     8821 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
     8881 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
     8941 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
     9001 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
     9061 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
     9121 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
     9181 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
     9241 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
     9301 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
     9361 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
     9421 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
     9481 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
     9541 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
     9601 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
     9661 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
     9721 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
     9781 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
     9841 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
     9901 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
     9961 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
    10021 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
    10081 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
    10141 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
    10201 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
    10261 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
    10321 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
    10381 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
    10441 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
    10501 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
    10561 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
    10621 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
    10681 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
    10741 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
    10801 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
    10861 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
    10921 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
    10981 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
    11041 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
    11101 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
    11161 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
    11221 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
    11281 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
    11341 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    11401 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    11461 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    11521 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    11581 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    11641 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    11701 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    11761 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    11821 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    11881 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    11941 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    12001 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    12061 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    12121 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    12181 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    12241 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    12301 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    12361 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    12421 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    12481 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    12541 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    12601 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    12661 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    12721 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    12781 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    12841 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    12901 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    12961 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    13021 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    13081 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    13141 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    13201 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    13261 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    13321 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    13381 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    13441 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    13501 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    13561 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    13621 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    13681 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    13741 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    13801 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    13861 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    13921 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    13981 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    14041 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    14101 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    14161 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    14221 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    14281 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    14341 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    14401 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    14461 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    14521 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    14581 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    14641 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    14701 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    14761 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    14821 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    14881 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    14941 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    15001 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    15061 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    15121 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    15181 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    15241 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    15301 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    15361 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    15421 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    15481 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    15541 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    15601 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    15661 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    15721 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    15781 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    15841 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    15901 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    15961 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    16021 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    16081 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    16141 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    16201 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    16261 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    16321 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    16381 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    16441 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    16501 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    16561 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    16621 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    16681 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    16741 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    16801 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    16861 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    16921 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    16981 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    17041 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    17101 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    17161 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    17221 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    17281 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    17341 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    17401 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    17461 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    17521 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    17581 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    17641 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    17701 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    17761 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    17821 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    17881 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    17941 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    18001 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    18061 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    18121 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    18181 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    18241 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    18301 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    18361 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    18421 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    18481 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    18541 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    18601 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    18661 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    18721 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    18781 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    18841 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    18901 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    18961 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    19021 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    19081 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    19141 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    19201 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    19261 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    19321 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    19381 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    19441 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    19501 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    19561 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    19621 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    19681 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    19741 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    19801 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    19861 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    19921 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    19981 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    20041 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    20101 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    20161 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    20221 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    20281 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    20341 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    20401 acaaggaaca atttcaaaaa
//
LOCUS       DASJOX010000330         6267 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.134, whole genome shotgun sequence.
ACCESSION   DASJOX010000330 DASJOX010000000
VERSION     DASJOX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.134"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..2406
                     /locus_tag="U4A12_002992"
     CDS             37..2406
                     /locus_tag="U4A12_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1775285.1"
                     /translation="MLTIGVGFALILFAVFGFLKLGFLGILIANGFRIIAGNTYQILC
                     LLLAVLGFWIVIKNTEFSIGKNRRWFGGILFYFGILLLLHAHLFGKLHTGEPNIMGTT
                     WDLLASDIKQSQVDNNVGGGMIGAILYHFTYFLIAQPGSYLAAVLLLAGGAFLFSNLE
                     GYQLLNGIQSIGERVQELLEGDPAKQARKQAAKEERMKQRAEAKEARRLAAKEAAEKE
                     AAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPANLEKPVPDTPK
                     QTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVDQSDEYKKIEK
                     NIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALALAAKD
                     VRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRVQTAD
                     LSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPHL
                     LTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPTL
                     PFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKAN
                     VPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVER
                     VVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRR
                     FRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            2636..3703
                     /gene="rlmN"
                     /locus_tag="U4A12_002993"
     CDS             2636..3703
                     /gene="rlmN"
                     /locus_tag="U4A12_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1775286.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3896..4999)
                     /locus_tag="U4A12_002994"
     CDS             complement(3896..4999)
                     /locus_tag="U4A12_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1775287.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5552..6100
                     /gene="aac(6')"
                     /locus_tag="U4A12_002995"
     CDS             5552..6100
                     /gene="aac(6')"
                     /locus_tag="U4A12_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1775288.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2074 a         1081 c         1352 g         1760 t
ORIGIN      
        1 aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg tgttgggttt
       61 gccttgattt tatttgctgt cttcggcttt ttaaaattag gttttttagg aatattgatt
      121 gcaaacggct ttcggatcat tgcaggaaat acttaccaaa tcctctgtct tttattagcc
      181 gtattaggat tttggattgt cattaagaat acagagtttt caattggtaa aaaccgacgc
      241 tggtttggcg gcatactctt ttatttcggt atcttattat tgttacatgc ccatttattt
      301 ggcaaactcc atactgggga accaaatatc atggggacga catgggattt gttagccagt
      361 gatataaaac aaagtcaagt agacaataac gttggtggtg ggatgattgg agcaatcctt
      421 tatcatttta cttacttctt gattgcacag cctggaagtt atttagcagc agtgctatta
      481 ctggcaggag gagcattcct gttcagtaat ctggaaggct atcagctgtt aaatggtatc
      541 caatcaattg gagaacgagt acaggaatta ttggaaggtg atcctgccaa gcaagctcga
      601 aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc
      661 ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg
      721 agccagcaaa gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag
      781 cagctaagtt ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag
      841 aaaccagtac cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg
      901 ccagaggatg acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac
      961 tatgaattac cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag
     1021 tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc
     1081 gatgccaagg tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg
     1141 gctgtagggg taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta
     1201 gcggcgaaag atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa
     1261 gtaccaaatg gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac
     1321 cacccagata aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg
     1381 gctgatcttt cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca
     1441 gtagcaatta atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa
     1501 ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg
     1561 ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa
     1621 gaaatggaag aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac
     1681 aatgaatttg ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc
     1741 atcgtagtga tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa
     1801 gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct
     1861 actcaacgtc caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga
     1921 atggcatttg cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca
     1981 gaaaagctct tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc
     2041 cgtgtgcaag gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca
     2101 gatcagcaag aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca
     2161 ggtgctccag aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa
     2221 atgcaaacgg caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca
     2281 gcacgtttag tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag
     2341 cctcggaaag tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac
     2401 atgtaaaact tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg
     2461 atgataccaa aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt
     2521 ctttttagtt tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag
     2581 gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga
     2641 aaaacagtcc atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg
     2701 cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa
     2761 tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat
     2821 catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt
     2881 gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt
     2941 gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg
     3001 gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt
     3061 ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg
     3121 gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga
     3181 tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca
     3241 caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc
     3301 gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa
     3361 attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata
     3421 tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt
     3481 aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca
     3541 tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa
     3601 aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg
     3661 acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt
     3721 tttcaattac cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga
     3781 gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc
     3841 aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac
     3901 tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa
     3961 tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg
     4021 ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta
     4081 acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact
     4141 gctgatccat catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc
     4201 cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat
     4261 agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt
     4321 gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag
     4381 tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta
     4441 gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac
     4501 ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta
     4561 caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac
     4621 ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag
     4681 ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca
     4741 ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg
     4801 aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta
     4861 ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat
     4921 attctttgac gattccattt gctttttcat acgcatctgt tccaatggta taatttggta
     4981 agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt
     5041 aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata
     5101 agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac
     5161 aagtacaaaa acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg
     5221 attccctttg cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa
     5281 acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca
     5341 tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga
     5401 ataaagtgtt tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga
     5461 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
     5521 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
     5581 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
     5641 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
     5701 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
     5761 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
     5821 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
     5881 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
     5941 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
     6001 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
     6061 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
     6121 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga
     6181 aattccaact tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa
     6241 aagtcgctta gtttcgagta gtaagaa
//
LOCUS       DASJOX010000331         5843 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.143, whole genome shotgun sequence.
ACCESSION   DASJOX010000331 DASJOX010000000
VERSION     DASJOX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.143"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            362..826
                     /locus_tag="U4A12_002996"
     CDS             362..826
                     /locus_tag="U4A12_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1775289.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            810..1439
                     /locus_tag="U4A12_002997"
     CDS             810..1439
                     /locus_tag="U4A12_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1775290.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1793..3271
                     /gene="msr(C)"
                     /locus_tag="U4A12_002998"
     CDS             1793..3271
                     /gene="msr(C)"
                     /locus_tag="U4A12_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1775291.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3318..3764
                     /locus_tag="U4A12_002999"
     CDS             3318..3764
                     /locus_tag="U4A12_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1775292.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3963..4478)
                     /locus_tag="U4A12_003000"
     CDS             complement(3963..4478)
                     /locus_tag="U4A12_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1775293.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4595..5797
                     /locus_tag="U4A12_003001"
     CDS             4595..5797
                     /locus_tag="U4A12_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1775294.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1811 a          972 c         1235 g         1825 t
ORIGIN      
        1 aaaaaaggtt gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc
       61 caatttctaa tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt
      121 aaaattatct actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt
      181 tttagcgttg tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc
      241 tatacttttc tattgagagc gtttttttta ttacacagct gattctttga cttttttctt
      301 tttttattct attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat
      361 tatgtcttta gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat
      421 cgtccagatc tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag
      481 actgaaagaa gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca
      541 aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag
      601 aaatcctcag aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat
      661 caagcattgt gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga
      721 tgtagaaatg aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat
      781 aacaacagaa acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta
      841 tgactggtag aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa
      901 tgctggaaat gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc
      961 gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca
     1021 atacgagaca aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt
     1081 acgaatatgg cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag
     1141 tcagagatca acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa
     1201 tgaacgatgt agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc
     1261 gtgatcatgg ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg
     1321 catttggttt ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg
     1381 tagaagaagg ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa
     1441 aggttttcag ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat
     1501 aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa
     1561 caactaaata aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat
     1621 tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt
     1681 tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct
     1741 cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa
     1801 tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag aactatctat
     1861 tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg
     1921 taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca
     1981 aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct
     2041 aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg
     2101 gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg
     2161 gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga
     2221 agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga
     2281 tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa
     2341 ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga
     2401 gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc
     2461 ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt
     2521 gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt
     2581 agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg
     2641 ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt
     2701 ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg
     2761 taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat
     2821 ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca
     2881 aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga
     2941 gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt
     3001 gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct
     3061 ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc
     3121 aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc
     3181 tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa
     3241 aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt
     3301 taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga
     3361 tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc
     3421 atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg
     3481 tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa
     3541 gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta
     3601 tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata
     3661 tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc
     3721 cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt
     3781 gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag
     3841 tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt
     3901 gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc
     3961 ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc
     4021 tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct
     4081 agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct
     4141 ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt
     4201 gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca
     4261 agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc
     4321 aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga
     4381 tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat
     4441 aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta
     4501 ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa
     4561 ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga
     4621 tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc
     4681 tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc
     4741 tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt
     4801 cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac
     4861 attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa
     4921 tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc
     4981 tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat
     5041 gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg
     5101 cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat
     5161 agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga
     5221 aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata
     5281 taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga
     5341 attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt
     5401 cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt
     5461 cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc
     5521 gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat
     5581 tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg
     5641 ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga
     5701 aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta
     5761 tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc
     5821 tgtcctgcat caagtcataa gaa
//
LOCUS       DASJOX010000332         5614 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.148, whole genome shotgun sequence.
ACCESSION   DASJOX010000332 DASJOX010000000
VERSION     DASJOX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.148"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..1027)
                     /locus_tag="U4A12_003002"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="U4A12_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="U4A12_003003"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="U4A12_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1775295.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="U4A12_003004"
     CDS             1852..3006
                     /locus_tag="U4A12_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1775296.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="U4A12_003005"
     CDS             3221..4189
                     /locus_tag="U4A12_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1775297.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="U4A12_003006"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="U4A12_003006"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1775298.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="U4A12_003007"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="U4A12_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DASJOX010000333         3554 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.192, whole genome shotgun sequence.
ACCESSION   DASJOX010000333 DASJOX010000000
VERSION     DASJOX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.192"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..737
                     /gene="erm(A)"
                     /locus_tag="U4A12_003008"
     CDS             6..737
                     /gene="erm(A)"
                     /locus_tag="U4A12_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEN1775299.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(863..1645)
                     /locus_tag="U4A12_003009"
     CDS             complement(863..1645)
                     /locus_tag="U4A12_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEN1775300.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1799..2176)
                     /locus_tag="U4A12_003010"
     CDS             complement(1799..2176)
                     /locus_tag="U4A12_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1775301.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(2183..>3554)
                     /locus_tag="U4A12_003011"
     CDS             complement(2183..>3554)
                     /locus_tag="U4A12_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1775302.1"
                     /translation="FYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
BASE COUNT         1090 a          686 c          516 g         1262 t
ORIGIN      
        1 ttataatgaa ccagaaaaac cctaaagaca cgcaaaattt tattacttct aaaaagcatg
       61 taaaagaaat attgaatcac acgaatatca gtaaacaaga caacgtaata gaaatcggat
      121 caggaaaagg acattttacc aaagagctag tcaaaatgag tcgatcagtt actgctatag
      181 aaattgatgg aggcttatgt caagtgacta aagaagcggt aaacccctct gagaatataa
      241 aagtgattca aacggatatt ctaaaatttt ccttcccaaa acatataaac tataagatat
      301 atggtaatat tccttataac atcagtacgg atattgtcaa aagaattacc tttgaaagtc
      361 aggctaaata tagctatctt atcgttgaga agggatttgc gaaaagattg caaaatctgc
      421 aacgagcttt gggtttacta ttaatggtgg agatggatat aaaaatgctc aaaaaagtac
      481 caccactata ttttcatcct aagccaagtg tagactctgt attgattgtt cttgaacgac
      541 atcaaccatt gatttcaaag aaggactaca aaaagtatcg atcttttgtt tataagtggg
      601 taaaccgtga atatcgtgtt cttttcacta aaaaccaatt ccgacaggct ttgaagcatg
      661 caaatgtcac taatattaat aaactatcga aggaacaatt tctttctatt ttcaatagtt
      721 acaaattgtt tcactaaatt aaagtaataa agcgttctct aatttcacaa gaggacgctt
      781 tattcttccc aaaaattgtt caatatttat caataaatca gtagttttaa aagtaagcac
      841 ctgttattgc aataaaatta gcctaattga gagaagtttc tatagaattt ttcatatact
      901 taacgagtgc tttcaccttt gaatatagtc cttcccactt atcatcacac tctccccgat
      961 agccttttct agctatatcc agtaaagtta catgctcttt aggtaaaaga ggtatagccc
     1021 attctgcagc gacatctttc gaggtaattt caccagtagt cactgtttgc cacattcgag
     1081 ctagggttaa aattacatta cgctcatcac cttttatccc ctcaattagt tctggcaaag
     1141 aatccttaat tgctcttcga atatctgtca aaggtacgga gacaagtata cttgaagaat
     1201 caggaccaaa tagagaaata ctattctttc ttgcttgtgc taaaacaata gccaaatcag
     1261 gatcatagct tggttcctga atttgtccat tctcaaattc acccctgagc cactcaccgt
     1321 atataaattc tctttttgga ggatattgcc aagggacaac ttcactccta tttataaccg
     1381 taacttcaag tggtctaaca gaatccgtat ttccaatctt tcctgatata gtcattagtc
     1441 tttctgttag tttttttcga gttaattgag gtaaactatg attcacgacg actagaacat
     1501 ctacatcgct gttaatgcgt aaaccaccat ttactgctga accaaataga tatactccaa
     1561 ctattgaact tccaaataaa tcttttacga tttttaatgt ttgaatcgct tgatttggta
     1621 tttttccgtt aatcaaattg ctcatgattt cacctcgtaa ctgattatgt tcatataaag
     1681 tttatattga tactcaattt acttacccta gattggacat atacctaaat gactgttcaa
     1741 taaagctgac cgttagcgtt taagtacatc ctttcacaat ttgtctacag attaataatt
     1801 attctttatt atacagatct ccatataatt tttgaatttg gttctgtaat tttttatttt
     1861 ctttttctaa ttccattact cttcttttta aggttttaat aaggatttcc tccgaacgag
     1921 aacttttctt gggttttgag actacatttg ctgttatttg acgctcacga agggattcga
     1981 ttctttgcct aatatcgtgt tccttataaa gccatgattt agaaacatta gcttcctttg
     2041 ctattgaatt aaaattaata actttacctt caatcgaaaa tttagaaatc gctttgtcta
     2101 ctttttccct tgtctttttt gatttctgct tcgccaaacg tacaatttct gttgtatttc
     2161 taacttgttt atccattgat aattaccccg tcaaacttcc aatgatttgt tctaaacgct
     2221 ctttaacacg gctattagtc tctacttgtc tttgccattg tttatcctta gctatggcta
     2281 ataactcttc tgtacgctct aactgttctt cgtgctgtgg taagaattgc ttactggtac
     2341 agaagtgagt gcaatctaag catgcattcg catgtggaca accacctgct actactggca
     2401 atctacaata accatttgga agcacttgtg catttatatt tttcttgaac cattgaagct
     2461 ctacatcatc gacttcatta tcttcatcta gatcaagcac atctccatta ttggtaacca
     2521 gtttttcctg aaatttagta aattcatttt ttagagtttc atcaaagata tgagcgtatc
     2581 tgcttgtcat ttctgggctt tcatgcccca aaaatttctg cacaatatgc tggggcatcc
     2641 cgttgttaat cattcttgtt cctactgtat ggcgaaaggc atgggcatgg aatctataaa
     2701 tctcacctga tttatccact atattttgct cataagctaa tttatttaac tcacctctaa
     2761 atgtttcttg ttttaatggc gatccatctt ttcttggaaa gaggtattca ctatctggaa
     2821 attcctctga aactttatct tcccgaactt taataagtaa agctacctct ttagatattg
     2881 gaactatatg ctcctttttc attttccatt gataatactt taaaaagaaa tctccatctt
     2941 tgtcctctaa tagacagcct tttttcaagg tgcacaattc acttatcctc attccacatt
     3001 cttgaacaat catagtcatc gtagctatat attcgggtaa tttatcaaga tgactgttca
     3061 attgctctag gacgaattca tctataaagc gtggttttgc tcttggtatt ttcggatagt
     3121 cctcagaata aattaatatt ttggaaggaa catcatccca ttctagccta agaagggtac
     3181 taaatagtcc ttccaatata gagatcctcc cagttattgt actaggtttt attcccatca
     3241 tgtttagttc acttaaatat gcttcaattt ccactctcgt taattggtgt actctctgaa
     3301 cttgtttaaa tttcatgtcc agaaaattaa agaactcttt aagtctttgg gcaatatcac
     3361 ttacatagga aaagctatcc atgttcaatc tcaacttaca atatcttttt acaagttgtt
     3421 taaaatatgt attccgaaac cctttaaagt taattgtata ttcatattgt gttgggttaa
     3481 ccttatcatc tggcaaaggt aagttacgtc tatcccaaac gtctttatcc cactcctctc
     3541 catcaaaata aaag
//
LOCUS       DASJOX010000334         2449 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.207, whole genome shotgun sequence.
ACCESSION   DASJOX010000334 DASJOX010000000
VERSION     DASJOX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.207"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            251..1753
                     /gene="eat(A)"
                     /locus_tag="U4A12_003012"
     CDS             251..1753
                     /gene="eat(A)"
                     /locus_tag="U4A12_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1775303.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1781..2347
                     /locus_tag="U4A12_003013"
     CDS             1781..2347
                     /locus_tag="U4A12_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1775304.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT          834 a          427 c          536 g          652 t
ORIGIN      
        1 aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
       61 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggaa
      121 gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct ttttgtgtgt
      181 atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag
      241 gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg acagccaagg
      301 cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac taggacttat
      361 cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca aactacctta
      421 tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga caaaagataa
      481 agaacgttta acctattacg tgttaaatga tattacggat tttgagatat gggaaatcga
      541 aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
      601 atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg acactcattt
      661 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac aagtagcggc
      721 ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg gatttatcga
      781 tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa
      841 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga
      901 aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc
      961 tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata ctgaatctag
     1021 acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac gatccaaagc
     1081 aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa aagatatcga
     1141 atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac ttttaagcgt
     1201 agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt ttacaatcga
     1261 gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat ccattatcca
     1321 ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc caaaacatct
     1381 gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg cggaatttgc
     1441 agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc ttgggatgga
     1501 aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga aaaaagtgga
     1561 attggctaaa tctttatcac agccagctga actatataca tgggatgaac cattgaatta
     1621 tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga aacctgccat
     1681 gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga ttatttctct
     1741 tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg ggaactttgc
     1801 caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc
     1861 acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac agatgcaggc
     1921 acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccattc
     1981 ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca gcttgccgct
     2041 gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac tgccaagcaa
     2101 agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa atggggagtc
     2161 agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg aagacgaaga
     2221 aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg cttattttca
     2281 tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat tggtttctta
     2341 tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc ttttgcttag
     2401 cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaac
//
LOCUS       DASJOX010000335         1624 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.238, whole genome shotgun sequence.
ACCESSION   DASJOX010000335 DASJOX010000000
VERSION     DASJOX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.238"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            126..623
                     /gene="dfrG"
                     /locus_tag="U4A12_003014"
     CDS             126..623
                     /gene="dfrG"
                     /locus_tag="U4A12_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1775305.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(695..>1624)
                     /locus_tag="U4A12_003015"
     CDS             complement(695..>1624)
                     /locus_tag="U4A12_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1775306.1"
                     /translation="RKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGK
                     ITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELR
                     LSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLT
                     SHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLN
                     VNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFC
                     RPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          532 a          239 c          303 g          550 t
ORIGIN      
        1 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
       61 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      121 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      181 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      241 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      301 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      361 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      421 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      481 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      541 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      601 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      661 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      721 aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg
      781 ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag
      841 catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta
      901 atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat
      961 ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg
     1021 ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt
     1081 tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat
     1141 gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga
     1201 atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc
     1261 attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc
     1321 ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa
     1381 tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag
     1441 tataaggagc atcaacattt aaaccatcga caaatctact cctattagta attttcccta
     1501 attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat
     1561 aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt
     1621 ttct
//
LOCUS       DASJOX010000336         1504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.243, whole genome shotgun sequence.
ACCESSION   DASJOX010000336 DASJOX010000000
VERSION     DASJOX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.243"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            262..345
                     /locus_tag="U4A12_003016"
                     /pseudo
     CDS             262..345
                     /locus_tag="U4A12_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="U4A12_003017"
     CDS             394..477
                     /locus_tag="U4A12_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEN1775307.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="U4A12_003018"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="U4A12_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1775308.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DASJOX010000337         1425 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.250, whole genome shotgun sequence.
ACCESSION   DASJOX010000337 DASJOX010000000
VERSION     DASJOX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.250"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            61..>1425
                     /gene="aph(2'')-I"
                     /locus_tag="U4A12_003019"
     CDS             61..>1425
                     /gene="aph(2'')-I"
                     /locus_tag="U4A12_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HEN1775309.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          602 a          103 c          245 g          475 t
ORIGIN      
        1 aaaaatgatt ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa
       61 atgaatatag ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg
      121 atgttaaaat ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa
      181 tatacattag aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga
      241 gtaattattg aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat
      301 gagttatata ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa
      361 tttataggag agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt
      421 tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat
      481 aatccaagag caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca
      541 gaacatgaat tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat
      601 gataatgcca caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc
      661 aaagtagata gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat
      721 aatgaataca tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa
      781 aaagcaatat ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt
      841 gaatattcgt atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact
      901 tttttaacac cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga
      961 gatattgcca gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt
     1021 actattgata ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat
     1081 aatgatttaa ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca
     1141 acaacagttt ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta
     1201 ttgttagatg gcaataatag attaactgga ataattgatt ttggagattc tggaattata
     1261 gatgaatatt gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat
     1321 tttggagaag atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat
     1381 caagatatag ttgaagaata ttatcctatt gaaactattg tttat
//
LOCUS       DASJOX010000338         1350 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.255, whole genome shotgun sequence.
ACCESSION   DASJOX010000338 DASJOX010000000
VERSION     DASJOX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.255"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..298
                     /locus_tag="U4A12_003020"
                     /pseudo
     CDS             47..298
                     /locus_tag="U4A12_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="U4A12_003021"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="U4A12_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1775310.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DASJOX010000339         1346 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.256, whole genome shotgun sequence.
ACCESSION   DASJOX010000339 DASJOX010000000
VERSION     DASJOX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.256"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..349)
                     /locus_tag="U4A12_003022"
     CDS             complement(<1..349)
                     /locus_tag="U4A12_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775311.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSI"
     gene            complement(1063..>1346)
                     /locus_tag="U4A12_003023"
     CDS             complement(1063..>1346)
                     /locus_tag="U4A12_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1775312.1"
                     /translation="KYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEVDEG
                     MKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          436 a          260 c          185 g          465 t
ORIGIN      
        1 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca tacttgcaaa
       61 gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc ataaagtgtt
      121 tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc ttataatgtt
      181 ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact acgtcatccg
      241 ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc cctattagta
      301 acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt tttatactcc
      361 ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa
      421 agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat
      481 agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag gacctatgga
      541 ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat attgctttta
      601 aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg
      661 tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt tacgactatg
      721 ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa tcctacaatt
      781 ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc ccccattgag
      841 cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc cctcaaatga
      901 gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat atatagattg
      961 aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc
     1021 aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta ttctttcatc
     1081 ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa ttttgggttt
     1141 ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat aacctgtaac
     1201 gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg aagcaatcaa
     1261 acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt gcaaagtacg
     1321 gatcttatct actgttccat attttt
//
LOCUS       DASJOX010000340         1194 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.270, whole genome shotgun sequence.
ACCESSION   DASJOX010000340 DASJOX010000000
VERSION     DASJOX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.270"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="U4A12_003024"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="U4A12_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN1775313.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DASJOX010000341         1072 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2797
            SAMN38756239-rid20573803.guided.284, whole genome shotgun sequence.
ACCESSION   DASJOX010000341 DASJOX010000000
VERSION     DASJOX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756239
            Sequence Read Archive: SRR27168105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028258.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028258.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:23:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756239-rid20573803.guided.284"
                     /isolate="VE2797"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-11-09"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..147
                     /locus_tag="U4A12_003025"
                     /pseudo
     CDS             1..147
                     /locus_tag="U4A12_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            178..1053
                     /locus_tag="U4A12_003026"
                     /note="aph(2'')-Ia"
     CDS             178..1053
                     /locus_tag="U4A12_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEN1775314.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          457 a           82 c          180 g          353 t
ORIGIN      
        1 gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa
       61 aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa
      121 gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg
      181 aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt
      241 agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt
      301 agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca
      361 aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta
      421 tctatactag gttataaaga aattaaagga acttttttaa caccagaaat ttattctact
      481 atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg
      541 cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta
      601 gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat
      661 tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt
      721 ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact
      781 ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt tatatactta
      841 cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat
      901 ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct
      961 attgaaacta ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga
     1021 aaagaaattt ataaaaggac ttataaagat tgattatata atatatgaaa ag
//
